Bioreactor Fluid Perfusion System
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Transcript of Bioreactor Fluid Perfusion System
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BIOREACTOR FLUID PERFUSION SYSTEM
Mechanical Engineering Capstone - 2010
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Project Sponsors Dr. Sean S. Kohles PSU Reparative
Bioengineering Lab
In collaboration with the National Institute of Standards and Technology (NIST), with grants from the National Institutes of Health (NIH).
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Background Millions suffer joint
tissue damage annually Bone Tendons Cartilage
If the damaged area is too large, it won’t heal
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Background Synthetic scaffold
materials Support cells Allow applied
loads to stress cells
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Background
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Interfacing EquipmentHigh Precision Linear Actuator
Displacement Guide “Hat”
Force Application Pistons with Load Cells (5)
Camera
Ultrasonic Transducer
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Interfacing Equipment Incubator – Used
to maintain temperature, humidity, O2 and CO2 levels.
Limits size of bioreactor equipment to inner chamber envelope (21”w x 17”d x 27”h)
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What’s the Need? Manual Refresh of
Solution Time consuming Requires
disassembly of the apparatus
Disrupts the equilibrium of the cell environment
Requires lab workers to be available at all hours
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What’s the Need? Automated Fluid Perfusion System
Circulate nutrient solution Interfaces with the existing system Works within the fully assembled system Adjustable timing to allow it to run
unattended for extended periods, “set it and forget it”
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Design – Requirements Environment
Temperatures up to 50°C Relative humidity up to 95% O2 and CO2 levels up to 20%
Function Up to 7 days unattended run time Run intervals adjustable from continuous to
72 hours Mininum of 4 mL/min flow rate
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Design – Requirements Users
Easy setup and takedown Fast replacement of parts to minimize
disruption to tissue samples Fabrication
Total budget of $1k Must be manufactured with PSU’s machine
shop
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Design – Conception
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Design – Conception “Dovetail” Advantages
Tool free assembly and disassembly
Simplified design to minimize manufacturing time and costs
Reduction in material usage
Allows future use of different size/shape sleeves with same mounting clip
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Design – Conception
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Design – Conception
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Design – Refinement Solution provides
nutrients, removes waste
Must not be allowed to stagnate
Initial design suffered from recirculation problems.
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Design – Refinement
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Design – Refinement Stress Analysis
Stress concentration points at neck
FEA Modeling of sample sleeve and tray
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Design – Realization
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Design – Realization
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Challenges What didn’t go as expected What was learned from those
challenges?
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ANY QUESTIONS?