Biopesticide seed treatments for improved growth of maize seedlings
20
© The Real IPM Company Thika, Kenya Biopesticide seed treatments for improved growth of maize seedlings Daniel M. Kang`ethe Research and Development Team, The Real IPM Company, (Kenya) Ltd First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25- 27 February 2013
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Presented by Daniel M. Kang`ethe at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013
Transcript of Biopesticide seed treatments for improved growth of maize seedlings
© The Real IPM Company Thika, Kenya
Biopesticide seed treatments for improved growth of maize
seedlings
Daniel M. Kang`etheResearch and Development Team, The Real IPM Company,
(Kenya) Ltd
First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27
February 2013
© The Real IPM Company Thika, Kenya
summary
- Objective
- Background
- Methodology/procedure
- Criteria of evaluating growth promotion.
- Assessment
- Conclusion
© The Real IPM Company Thika, Kenya
Back ground
Consortium of:
Jomo Kenyatta University of Agriculture and Technology (Kenya) (JKUAT)
Alpha Seeds, Tanzania.
The Real IPM Company (K) Ltd, Kenya
© The Real IPM Company Thika, Kenya
Real IPM
Biocontrol company based in Thika, Kenya
Delivery of Biopesticide on the seed.
Many advantages of seed delivery
© The Real IPM Company Thika, Kenya
Research Objective
To assess the growth promoting effect of Biopesticides (T. asperellum, B. subtilis, M. anisopiliae and T. asperellum + M. anisopiliae) as seed treatment on maize.
© The Real IPM Company Thika, Kenya
Procedure
Origin of Biopesticides
Formulated specially for seed coating.
Maize was used as test plant.
Manufactured by Real IPM Kenya.
Design for on farm application
© The Real IPM Company Thika, Kenya
Procedure cont…
© The Real IPM Company Thika, Kenya
Procedure cont..
© The Real IPM Company Thika, Kenya
Procedure cont…
Above plate pictures of (a) Seed coated maize sample and the control and (b) colony growth
© The Real IPM Company Thika, Kenya
Treatments
1. T. asperellum2. M. anisopiliae 3. B. subtilis4. T. asperellum + M. anisopiliae 5. Control
Germination and seedling growth assessments
© The Real IPM Company Thika, Kenya
Parameters
• Germination• Tap root length • Number of feeder roots• Shoot height • Fresh weight of the seedlings
© The Real IPM Company Thika, Kenya
Results - Germination
0
20
40
60
8079.6
54
83.0777.33
83.33
TREATMENTS
GER
MIN
ATIO
N %
© The Real IPM Company Thika, Kenya
Shoot length
BACILLUS CONTROL MET + TRICHO
METARH TRICHO0
1
2
3
4
5
6
7
8
9
10
7.27
4.72
8.31
7.34
8.92
TREATMENTS
SH
OO
T LEN
GTH
(cm
)
© The Real IPM Company Thika, Kenya
Tap root length
BACILLUS CONTROL MET + TRICHO
METARH TRICHO0
5
10
15
20
25
19.21
12.01
21.5320.53
15.59
TA
P R
OO
T L
EN
GTH
(cm
)
© The Real IPM Company Thika, Kenya
Number of feeder roots
BACILLUS CONTROL MET + TRICHO
METARH TRICHO0
1
2
3
4
5
6
7
5.6
4.4
5.27
5.93
5.4
TREATMENTS
NU
MB
ER
OF F
EED
ER
RO
OTS
© The Real IPM Company Thika, Kenya
© The Real IPM Company Thika, Kenya
Assessment
Biopesticides cfu`s/ Maize seed
Trichoderma asperellum 9.2 X10 7 Bacillus subtilis 7.5 X10 6
Metarhizium anisopliae 1.3 X10 6
How many microbes on the seed?
© The Real IPM Company Thika, Kenya
Conclusion
Biopesticides enhance growth
Act as growth promoters
Widely reported effect
Complex mechanism
Other pest and disease benefits(See JKUAT paper)
© The Real IPM Company Thika, Kenya
Conclusion
What next
Cropping trials have begun
Seed company application (as opposed to on farm treatment)
Looking for seed company partners in the region
Please contact Real IPM/me
