BioN OCTM II Carriers - wakenbtech.co.jp · Cell Culture Matrix BioN OC II Manufactured by 100% PET...
Transcript of BioN OCTM II Carriers - wakenbtech.co.jp · Cell Culture Matrix BioN OC II Manufactured by 100% PET...
BioN OCTM II Carriers
PUREPURELIGHTERLIGHTERHIGHER S/HIGHER S/VVCOST EFFECTIVECOST EFFECTIVE
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Cell Culture MatrixCell Culture Matrix
BioNBioNOOC IIC II
Manufactured by 100% PET non-woven fabrics
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Features� PURE, 100% pure PET material
� LIGHT WEIGHT, 1 gram of carriers occupy 15 ml space
� High Surface to Volume Ratio , S/V ~ 150 cm -1
� High Capacity , 1 gram carriers provide space for over 1x10 9 cells
to propagate
� Special surface treatment to enhance biocompatibi lity.
Hydrophilicity keeps stable for at least two years.
� Special geometric design to enhance mixing and supp ort load-
bearing capacity in packed-bed.
� Render autoclave, gamma- irradiation, and EO sterili zation
treatment
� Non-pyrogenic, non-cytotoxic
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Quality
�100% Pure Material; regulation friendly.
�Raw material passed FDA approval .
� Provide complete resource documentation for .
�Provide Certificate of Quality document for each
purchase
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
BioNBioNOOC IIC II
120 cm 2/ml300 cm 2/mlHigher S/VHigher S/V
0.8 mg/cm 20.2 mg/cm 2LighterLighter
50%PET/50%PP100% PETPurerPurer
CompetitorBioNOC II
and Cost effectiveand Cost effective ……
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Geometric Design
�Increase load-bearing capability to support packed-bed structure.
�Increase void fraction and reduce diffusion limitation.
�45o Angle generates microscopic eddy for mixing.
45o
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Applications� fit fixed bed cell culture system .
�BelloCellTM culture device
�TideCellTM culture device
�any commercial packed bed culture device
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Comparison DataComparison DataCommercial disks and BioNOC II
®
carriers
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Test Systems
• BelloCell 500 BioNOC II ® Commercial Disks
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Microcarrier Characters
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
BioNOC II ® Commercial Disks
Material 100% PET PET/PP
Dimension 5 mm X 10 mm strip Disk diameter 6 mm
Specific Surface area 2,400 1,200
Packed volume (ml/g) 15 10
S/V 160 120
Autoclavable OK OK
Gamma irradiation OK
Endotoxin tested (EU/ml) <0.25 < 0.5
Bioburden (CFU/gram) < 1 < 0.36
Cytotoxicity tested serum and serum-free culture test
Yes
Regulation guideline USP <87><88>, ISO10993-5 USP <87><88>, ISO10993-5
Test Conditions (1)
BioNOCII ®
Cell Line::::HEK 293
Medium:::: ααααMEM+10%FBS
Medium volume: 500 ml
Seeding : 1.3××××108 cells
Culture Mode: Fed-Batch
Commercial Disks
Cell Line::::HEK 293
Medium:::: ααααMEM+10%FBS
Medium volume: 500 ml
Seeding : 1.3××××108 cells
Culture Mode: Fed-Batch
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Growth Curve total cell No.
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
-48 -24 0 24 48 72 96 120144168192216240264288312336360384
0.0
5.0x108
1.0x109
1.5x109
2.0x109
2.5x109
3.0x109
3.5x109
4.0x109
Cel
l Pop
ulat
ion
(cel
ls/b
ottle
)
Culture Age (hours)
BioNOC II, 6.5 g FibraCel, 10 g
Growth Curve cell No. per gram carriers
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
-48 -24 0 24 48 72 96 120144168192216240264288312336360384
0
1x108
2x108
3x108
4x108
5x108
6x108
cell
popu
latio
n pe
r gr
am c
arrie
rs
Culture Time (hours)
BioNOC II FibraCel
BioNOC II provide up to 40% higher cell density compared with Fibra-Cel disks.
Cell Mass Production
CESCO Bioengineering Co., Ltd. www.cescobio.com.tw
Total cells
Cells/gram carrier
0.05.0x108
1.0x109
1.5x109
2.0x109
2.5x109
3.0x109
3.5x109
4.0x109
Fin
al C
ell P
opul
atio
n (c
ells
/bot
tle)
BioNOC II carriers FibraCel disks
0
1x108
2x108
3x108
4x108
5x108
6x108
Cel
l den
sity
(ce
lls/g
car
rers
)