Biomolecular and Cellular Engineering
8
Biomolecular and Cellular Engineering Microbes Enzymes Biomolecules Metabolic Engineering Biopolymer Antibody Nano-Devices, Sensors, Environmental, Biosynthetic and Biomedical Applications Synthetic Biology Protein Engineering Directed Evolution
description
Biomolecular and Cellular Engineering. Microbes Enzymes Biomolecules. Metabolic Engineering . Synthetic Biology . Protein Engineering . Directed Evolution . Biopolymer Antibody. - PowerPoint PPT Presentation
Transcript of Biomolecular and Cellular Engineering
Biomolecular and Cellular Engineering
Microbes Enzymes Biomolecules
Metabolic Engineering
Biopolymer Antibody
Nano-Devices, Sensors, Environmental, Biosynthetic and Biomedical Applications
Synthetic Biology
Protein Engineering
Directed Evolution
Research Interest
Biomolecular Tools
Artificial Cellulosomes
1. Found in anaerobic microbes 2. Hydrolyze cellulose up to 50-fold faster due to synergy3. Interaction between dockerin to cohesin is species specific
Bayer et al., 2006
Synthetic Yeast Consortia
7000
8000
9000
10000
11000
0 20 40 60 80
Rem
aini
ng S
ugar
s (m
g/L)
Time (hrs)
Control
Free Enzymes
Cellulosome
0
200
400
600
800
1000
1200
0 20 40 60 80
Etha
nol (
mg/
L)
Time (hrs)
Control
Free Enzymes
Cellulosome
5
10
15
20
25
0 20 40 60 80
Cell N
umbe
rs (1
06 /m
L)
Time (hrs)
Control
Free Enzymes
Cellulosome
YEASTYEAST
YEASTYEAST
YEASTYEAST
His6 Tag
His6 Tag
His6 Tag
YEAST
AGα 1CBDC T F
YEAST
AGα 1CBDC T F
AGα 1CBDC T F
Virus detection and drug discovery
Influenza
In situ Monitoring of Virus Replication Using Molecular Beacons
Nucleus
UncoatingTranslation
RNA replication
Fixation with para-formaldehyde and permeabilization Triton X-100
In vivo detection of Coxsackie B6 virus in BGMK cells
PlasDIC (t = 0 min)
PlasDIC (t = 18 hr)
Cells infected with 103 PFU, M.O.I.≈ 0.5 (15 min to 18 hr)
Yeh et al. PNAS, 17522-17525, 2008
Acknowledgement
9 Graduate Students and 1 Postdoc
