Biomedical Engineering Society FY10 Financial Budgeting Process
43
“What I did Last Summer” REU & Internship Experiences November 23, 2009 6pm, SL 104
Transcript of Biomedical Engineering Society FY10 Financial Budgeting Process
“What I did Last Summer”REU & Internship Experiences
November 23, 2009
6pm, SL 104
Introducing…
REU’s, Internships, Research Experiences by…
• Carol Wood
• Deepti Kalluri
• Funmi Adebayo
• Greg Lucini
• Angelica DeMartino
• Lydia Bakalova
• Liz Tuite
Interdisciplinary Materials REU
Selective Interactions of
Charged Nanoparticles
1.2
0.8
0.4
0.0
Ab
so
rban
ce (
AU
)
750500
Wavelength (nm)
Before Filtration After Filtration
1.8
1.6
1.4
1.2
1.0
0.8
0.6
0.4
0.2
Ab
so
rba
nce
(A
U)
12080400
Time (hours)
Control enTMOS
Benefits
1. Awesome
2. Shenanigans
3. Networking
4. Science
5. Dolla’ Dolla’ Billz
6. Friends!
7. Fun!
University of Texas Health Science
Center at San Antonio
Physiology Undergraduate Research
Experience (PURE)
Background
• Synaptic connections are being continuously
remodeled
– Responsible for learning and memory
• Loss of connections leads to neurodegenerative
diseases
My Project
• Role of Darf2 in Synapse Growth and Protein
Localization
• 2 domains
• Which domain is responsible for loss of synaptic
connections?
– Decreased growth
– Altered protein localization
Results
Mutant 1 Mutant 2
• Domain 1 is responsible for normal synaptic function
Wild Type
What I got out of it
• Opportunity to present my poster at a poster
session
• Learned a lot about genetics/ neuroscience/
physiology
• Journal clubs & luncheon sessions with graduate
students and faculty
How I got it
• http://people.rit.edu/~gtfsbi/Symp/summer.htm#skip
• Volunteering in a lab
• Application, personal statement, recommendations,
transcript
WPI Bioengineering REU
WPI Bioengineering REU
•10-week Program
•Housing was provided
•$4000 Stipend
Research
•Tissue Rings
•Vascular Tissue
Engineering
•Cell-Derived Rings vs.
Biopolymer Gel rings
•Mechanical Testing,
Histology
Other Knowledge
• How to write an abstract
• PowerPoint Presentations
• How to write a resume
• Applying to graduate schools
BioDiscovery
BioDiscovery
• One-week program
• Mentored middle-school girls in
Biomedical Engineering research
• So much fun!!!
Tons O’ Fun!
BMES Conference – Pittsburgh PA
Mass Life Sciences Center Internship
Challenge- Bristol-Myers Squibb
• How did I get the job?
– MLSC Internship Challenge
– Government encouraged biotech companies to hire
interns
– Paid internship 8 weeks for $4800 ($15/hour)
• ^^^ pretty decent
What I did last Summer?
Lydia Bakalova
November 23rd, 2009
The company
• Located in Raynham, MA
• Have 4 divisions of DePuy
– Orthopaedics – hip, knee, shoulder implants
– Mitek – sports medicine (tendons, ligaments,
anchors)
– Spine – spinal care
– Codman – neurosurgical therapies
DePuy Orthopaedics
• Manufacture knee, hip and
shoulder implants
• Cleaning process
• Passivation (if applicable)
• Package
• Send for sterilization
http://www.kneereplacement.com/binary/org/DEPUY/images/joint/knee/product/Sigma_rpf_LargeImage.jpg
http://www.biomedcentral.com/content/figures/1471-2474-9-147-2-l.jpg
What I did?
• Join the Manufacturing Division
– Clean & Pack team
• Co-op May-Dec. 2008
• Intern May-Aug. 2009
http://www.globalpackagegallery.com/main.php?g2_view=core.DownloadItem&g2_itemId=47298&g2_serialNumber=3
Co-op Responsibilities
Responsibilities
Validation of new packaging
configurations
Creating and executing DOEs
(Design of Experiments)
Validate and document any
repairs or changes
Ensure the equipment meets the company and
FDA requirements
Intern Responsibilities
• Involved in LEAN manufacturing project
– Video-tape manufacturing process
– Brake-down tasks
– Doing time analysis
– Create new plan for time and error reduction
– Validate the concept
– Rearrange the work stations
– Train the operatiors
What are the benefits?
Benefits
NETWORKING!
Meet with many people
Make impression
Possibility for long term
job offer
Industry exposure
Work independent and in team
Communication
Different departments
Different educational/
hierarchy levels
Operators, maintenance technicians, managers
Management, engineering,
sales
How I found the opportunity?
• Co-op:
– Applied on the JnJ website
– Received a call for co-op interview
– Got the job
• Intern:
– Made good impression
– Networking
– Received e-mail from previous supervisor
– Got the job without interview
Questions?
Words of Advice
• Apply Early!!
• Apply to as many REU’s as you can
• Ask your professors for advice
• They can help you!!
• You will meet new people anywhere you go
•Make the best of your experience
How did I get involved in this program?
• Submitted resume online to MLSC
– Really easy, just upload resume
– No extra stuff to complete
• Waited
– I found out about MLSC really late.
– Kept waiting
• Mid-June
– Phone call!
– Asked to setup phone interview
– I had a job offer within four days of initial contact
What did I do at BMS?
• Worked in Devens, MA
• BMS’s brand-new under construction large scale
cell culture facility
• Produced biologics
– Primarily Orencia, a protein-based drug used to treat
rheumatoid arthritis.
BMS? That sounds like Chem Eng?
• Manufacturing Engineering/Technical Services
– Maintained and operated the bioreactor equipment
– Learned a lot about chem eng stuff too!
• Corporate Environment
• Regulated Environment
– Document Control, Quality Control, Validation testing,
startup testing
– Strict government regulations on pharmaceutical
companies, had to adhere to them
Women & Infant’s
Molecular Biology Lab
Kilguss Research Institute
Summer 2009
Testing novel drugs for ovarian cancer
Cells:
• SKOV-3 Cells
Drugs:
• PACs
• MT19C
– Vitamin D Derivative
Culturing Cells
Bradford Assay
• Determining Protein Concentration
Western Blotting
What Does
This Mean??
DAPI/TUNEL Assay
Benefits of Doing an Internship
• Gained valuable lab experience
• Learned about writing grant proposals and
manuscripts
• Worked with people from different backgrounds
Intern at Myomo, Inc.
• How did I get the internship?
Intern at Myomo, Inc.
• What did I do? Quality
testing!
User-interface
testing!
SBIR Grant!
Buying!
Interact
with
patients!
BenefitsStart-up Company Experience
Worked directly w/ CEO & DOE
Made some $$
Contacts & references
Met Shawn Thornton @ the gym in my office complex
