Biology Discussion Notes Wednesday 3/4/2015. Goals for Today Be able to name and describe the two...
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Transcript of Biology Discussion Notes Wednesday 3/4/2015. Goals for Today Be able to name and describe the two...
Biology Discussion Notes
Wednesday 3/4/2015
Goals for Today• Be able to name and describe the two major steps of
protein synthesis (how proteins are made)
• Be able to list the molecules & structures involved in Transcription & Translation.
• Be able to describe the differences between DNA & RNA
“Question” of the Day 3/04
• What process makes an mRNA molecule?
• What is needed to produce it?
• What sets mRNA apart from DNA? (Look at QoD from yesterday if you can’t remember)
Messenger RNA - mRNA
DNA’s “recipes” are transcribed by the enzyme ____ ____________ to make a molecule of mRNA.
mRNA is like a different language then DNA
mRNA is the language that the ribosome -(protein building machine) can read.
Steps of Protein Synthesis• The next major process in protein synthesis is known as
“translation” in this process mRNA is translated into protein.
2. Before translation can happen mRNA must move out of the nucleus ( in __________ organisms) and into the cytoplasm. This is a crucial step because RIBOSOMES (our cell’s __________ factories) are only found outside the nucleus.
Steps of Protein SynthesisThe next stage of Protein Synthesis is TRANSLATION
Steps of Protein Synthesis
If someone asked you to “Translate” something what would that mean, what would they be asking you to do?
What “languages” are we using?
Steps of Protein Synthesis
• The next step of Protein Synthesis is TRANSLATION
3. Once the mRNA strand leaves the nucleus and enters
the cytoplasm the mRNA Attaches to a ribosome (our cell’s __________making machine)
– Made of RIBOSOMAL RNA - rRNA
Steps of Protein Synthesis
3. Once the mRNA strand leaves the nucleus and enters the cytoplasm the mRNA Attaches to a ribosome (our cell’s protein making machine)
4. Next a molecule of Transfer RNA (tRNA) attaches to the mRNA strand
Anatomy of a Transfer “t” RNA (tRNA)Draw one in your notes!!!
Steps of Protein Synthesis
4. Next a molecule of Transfer RNA (tRNA) attaches to the mRNA strand
Steps of Protein Synthesis
4. Next a molecule of Transfer RNA (tRNA) attaches to the mRNA strand
5. Amino acids are linked together by the ribosome to form the final protein (which is really just a long chain of amino acids)
Protein Synthesis
Lets put it all together!
Create the mRNA, tRNA and find the Amino Acid coded for on mRNA
Pick a decoder
Create the mRNA, tRNA and find the Amino Acid coded for on mRNA
Create the mRNA, tRNA and find the Amino Acid coded for on mRNA
Important!…..
• The genetic code is REDUNDANT
• but NOT AMBIGUOUS (Unambiguous)
What else besides Amino Acids do you see in your chart?
How do you figure these things out?
• Any ideas how you would start if you don’t know any of this?
Nobel WinnersPhysiology & Medicine 1968
The Nobel Prize in Physiology or Medicine 1968 was awarded jointly to Robert W. Holley, Har Gobind Khorana and Marshall W. Nirenberg "for their interpretation of the genetic code and its function in protein synthesis".
Is this genetic code Universal?
• Do all organisms have DNA?
• Do all organisms have RNA?
• Do all organisms’ RNA code for proteins in the same way (Same codon = same AA)?
The (near) universality of the genetic code is what allows transformation to work.
DNA from one organism can be put into another (AND FUNCTION) in that other organism!!!!
If I insert the gene (code for 1 _______ ) into another organism, what should that organism be able to do with it?
The (near) universality of the genetic code
If I insert the gene (code for 1 _______ ) into another organism, what should that organism be able to do with it?
MAKE PROTEINS CODED FOR
The (near) universality of the genetic code
Transformation Lab
Transformation Lab
The (near) universality of the genetic code is what allows –transformation
to work.
National Geographic Story:
• http://news.nationalgeographic.com/news/2009/05/photogalleries/glowing-animal-pictures/
“With great power comes great responsibility!”
• Next week we will discuss what this technology can do, and debate what we should or shouldn’t do with it.
On 3/04/2015 I _______ ________ learned how to decode the code in all* living things.
*almost all
Let’s simplify before we complicate
Protein Synthesis Story
• Go over page 2 – Questions?
Protein Synthesis Story
• Complete through #5 on page 3 for tomorrow