Biology 102 - Linn–Benton Community Collegecf.linnbenton.edu/mathsci/bio/millern/upload/Chemistry...
Transcript of Biology 102 - Linn–Benton Community Collegecf.linnbenton.edu/mathsci/bio/millern/upload/Chemistry...
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Biology 102
Instructor: Nate Miller
Email: [email protected]
Phone: 541-754-6352
Office : White Oak Hall 220
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Note Card
•Name
•Goals
•Favorite Scientist
•Science education background.
•Something interesting (hobbies, favorite joke, celebrity you most resemble, etc.) Anything
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Goals
•Gain a better understanding of biological systems
1. Cell structure and function
2. Cell division and inheritance
3. Evolutionary processes
•Understand the scientific method (scientific inquiry)
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Extra Credit Read a chapter of a popular biology book and write a one page summary of it.
Digital format
2%
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Suggestions Genome
Diversity of life
The beak of the finch
The advance of the fungi
Darwin’s dream pond
The botany of desire
The Immortal Life of Henrietta Lacks
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Biology 102 Class Structure
Grading: Percentage of final grade:
10 Labs, drop the lowest one = 10% Other in class Activities = 15% Online Homework = 10% 2 midterm exams = 40% Final Comprehensive exam = 25%
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Expectations
•Respect towards instructor and fellow students
•I respect you
•Interaction and participation with instructor and fellow students
•Sincere interest and enthusiasm for learning
•Effort
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Policies
No Cell Phones
Lap tops are OK for class use
Emergency situations can have exceptions
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Policies
Grading Scheme:
90 - 100% A
80 – 89% B
70 – 79% C
60 – 69% D
59.9 % and below F
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Biology: the study of life
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What is Life?
Definition
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What is Life? •Growth
•Reproduction
•Adaptation
•Metabolism
•Cellular
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Cells
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Tree of Life
BACTERIA ARCHAEA EUKARYA
animals fungi
plants
protists
FIRST CELLS
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The scientific method
The car won't start.
Why won't the car start?
The car won't start because the battery is dead.
Propose a new hypothesis.
IF the hypothesis is correct, THEN the car will start if the battery is replaced.
The battery is replaced. If the car doesn’t start: If the car starts:
The dead battery hypothesis is supported.
Observation
Question
Hypothesis
Prediction
Experiment or Observation
Conclusion
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The experiments of Francesco
Redi
Experimental variable:
gauze prevents the entry of flies
Controlled variables:
time, temperature, place
Results
Control situation Experimental situation
Place meat in each jar
Obtain identical pieces of meat and two identical jars
Flies swarm around and maggots appear
Leave the jar uncovered
Leave exposed for several days
Flies are kept from the meat;
no maggots appear
Cover the jar with gauze
Leave covered for several days
Experiment:
Observation: Flies swarm around meat left in the open; maggots appear on the meat.
Question: Where do maggots on the meat come from?
Hypothesis: Flies produce the maggots.
Prediction: IF the hypothesis is correct, THEN keeping the flies away from the meat will prevent the appearance of maggots.
Conclusion: The experiment supports the hypothesis that flies are the source of maggots and that spontaneous generation of maggots does not occur.
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Luck and observation
A Petri dish contains solid growth medium
Bacteria grow in a pattern created by streaking
A substance from the mold diffuses outward and inhibits the growth of nearby bacteria
A colony of the mold Penicillium
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Chemistry
•Atoms •Protons
•Neutrons
•Electrons
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(a) Hydrogen (H)
electron shell
atomic nucleus
e
p
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(b) Helium (He)
n n
e
p p
e
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Carbon (C) Oxygen (O) Phosphorus (P) Calcium (Ca)
Ca O C P
4e 6e 5e
2e
8e
8e
8e
2e 2e
2e
2e
6p 8p 15p 20p
6n 8n 16n 20n
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heat energy
light
An electron absorbs energy
The energy boosts the electron to a higher-energy shell
The electron drops back into lower-energy shell, releasing energy as light
1 2
3
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Bonds •Covalent •Polar
•Nonplolar
•Ionic
•Hydrogen
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Hydrogen gas (H2)
_ _
(a) Nonpolar covalent bonding in hydrogen
gas (H2)
(hydrogens: uncharged)
+ +
Electrons spend equal time near each nucleus
Same charge on both nuclei
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Water
(b) Polar covalent bonding in water (H2O)
+
(+) (+)
(oxygen: slightly negative)
(hydrogens: slightly positive)
8p+ 8n
+
_ _ _
_ _
_
_ _ _ _
Larger positive charge
Electrons spend more time near the larger nucleus
Smaller positive charge
(–)
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Macromolecules
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Macromolecules
•Carbohydrates
•Lipids
•Proteins
•Nucleic acids
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Monosaccharides
galactose fructose
6
5
4
3 2
1 2
3 4
5
1
6
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Dehydration synthesis
dehydration synthesis
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Hydrolysis
hydrolysis
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Disaccharide
glucose fructose sucrose
dehydration
synthesis
•
•
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Starch polysaccharide
(b) A starch molecule
(a) Potato cells
(c) Detail of a starch molecule
starch grains
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Potato cells
(a) Potato cells
starch grains
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Cellulose
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Wood is mostly cellulose and lignin
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Lignin
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Chitin
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Lipids
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Triglyceride
glycerol
fatty acids
triglyceride
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A fat and an oil
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Phospholipids
polar head glycerol backbone
phosphate group
variable functional
group
(hydrophilic) (hydrophobic)
fatty acid tails
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Steroids
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Protein
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Amino acid structure
amino group
hydrogen
variable group
carboxylic acid group
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Protein synthesis
amino acid
amino group
amino group
carboxylic acid group
amino acid peptide water
peptide bond
•
dehydration
synthesis
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Amino acid diversity
glutamic acid (glu) aspartic acid (asp)
(a) Hydrophilic functional groups (b) Hydrophobic functional groups
(c) Sulfur-containing
functional group
leucine (leu) phenylalanine (phe)
cysteine (cys)
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Sulfur-containing functional group
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The four levels of protein structure (a) Primary structure:
The sequence of amino acids linked by peptide bonds
(c) Tertiary structure:
Folding of the helix results from hydrogen bonds with surrounding water molecules and disulfide bridges between cysteine amino acids
(d) Quaternary structure:
Individual polypeptides are linked to one another by hydrogen bonds or disulfide bridges
(b) Secondary structure:
Usually maintained by hydrogen bonds, which shape this helix
helix
hydrogen bond
heme group
leu
val
lys
lys
gly
his
ala
lys
val
lys
pro
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Nucleic Acids
• Adenine
• Thymine (Uracil)
• Guanine
• Cytosine
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Five-carbon monosaccharides
ribose deoxyribose
5
4
3 2
1
5
4
3 2
1
Note “missing” oxygen atom
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Deoxyribose nucleotide
sugar
phosphate
base
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The energy-carrier molecule adenosine triphosphate (ATP)
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Deoxyribonucleic acid (DNA)
hydrogen bond
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What is a cell? • Smallest unit w/ property of life
– Single-celled organisms
– Make up multicellular organisms
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The Cell Theory
1. All organisms composed of cells
2. Smallest unit of life
3. Division of preexisting cell
4. Possess heritable material (DNA)
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Cytoplasm
• Aqueous material
plasma
membrane
cytoplasm
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mitochondrion
cytoplasmic fluid
flagellum
vesicle
ribosomes on rough ER
centriole
intermediate filaments (cytoskeleton)
Golgi apparatus
cytoplasm
lysosome
exocytosis of material from the cell
polyribosome
nuclear pore
basal body
nuclear envelope
chromatin (DNA) nucleolus
nucleus
plasma membrane
rough endoplasmic reticulum
free ribosome
smooth endoplasmic reticulum
microtubules (cytoskeleton)
microfilaments
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central vacuole
cytoplasmic fluid plastid
vesicle
lysosome
cytoplasm
plasmodesmata
cell wall plasma membrane
intermediate filaments (cytoskeleton)
free ribosome
ribosomes
nucleus
nucleolus
nuclear pore chromatin
nuclear envelope
smooth endoplasmic reticulum
rough endoplasmic reticulum
Golgi apparatus
chloroplast
mitochondrion
microtubules (cytoskeleton)
cell walls of adjoining plant cells
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plasma
membrane
DNA in
nucleoid region
cytoplasm, with
ribosomes
Cell wall outside the
plasma membrane
Prokaryotic Cells
Fig 4.4
Capsule
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Eukaryotic cell NUCLEUS
PLASMA
MEMBRANE
CYTOPLASM
w/
RIBOSOMES
Fig 4.4
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How big are cells? • Most cells can be seen with light microscope
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Cell Membranes
• Fluid
• Composed of phospholipids, proteins, etc.
• Semipermeable
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Triglyceride
glycerol
fatty acids
triglyceride
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A fat and an oil
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Phospholipids
polar head glycerol backbone
phosphate group
variable functional
group
(hydrophilic) (hydrophobic)
fatty acid tails
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phospholipid
hydrophilic heads
hydrophobic tails
hydrophilic heads
extracellular fluid (watery environment)
cytoplasm (watery environment)
bilayer
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Kinks increase fluidity
more fluid less fluid
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Steroids
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lysosome
Food
Damaged component
digestion
Endomembrane System
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Exocytosis
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Chloroplast • Found in plants
• Sugar production
– Photosynthesis
Chloroplast
Central Vacuole
Storage of water and waste
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Mitochondria
• Energy producer of cell (ATP)
• Two membranes – allows for gradient formation
• Where cellular respiration occurs Outer membrane Inner membrane
outer compartment inner
compartment
Mitochondria
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Cells and cell membranes
• Prokaryotic vs. Eukaryotic
• Nucleus and other organelles
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glycoprotein
carbo- hydrate
extracellular fluid (outside)
cytoplasm (inside)
cholesterol
membrane protein channel protein
cytoskeleton
A phospholipid bilayer helps to isolate the cell's contents
Proteins help the cell communicate with its environment
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Diffusion of a dye in water
A drop of dye is placed in water
Dye molecules diffuse into the water; water molecules diffuse into the dye
Both dye molecules and water molecules are evenly dispersed
drop of dye
water molecule
2
1 3
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Diffusion and Osmosis
• Molecules move from high density to low density (diffusion)
• Diffusion across a membrane is Osmosis
• Some molecules can’t cross the cell membrane without active transport.
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Types of diffusion through the plasma membrane
water glucose
carrier protein
aquaporin channel protein
phospho- lipid bilayer
(cytoplasm)
(extracellular fluid)
Cl–
O2
(a) Simple diffusion through
the phospholipid bilayer (b) Facilitated diffusion
through channel proteins (c) Osmosis through
aquaporins or the
phospholipid bilayer
(d) Facilitated diffusion through
carrier proteins
Step 1
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The effect of solute concentration on osmosis
No net flow of water
Water flows out; the balloon shrinks
Water flows in; the balloon expands
(a) A balloon in an
isotonic solution (b) A balloon in a
hypertonic solution (c) A balloon in a
hypotonic solution
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The effects of osmosis on red blood cells
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DNA vs. RNA
• DNA uses deoxyribose and RNA uses ribose
• RNA has the nucleotide urical instead of thymine
• DNA is much more stable than RNA
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DNA to RNA
• DNA is the blueprint
• The code for individual genes gets copied to RNA
• RNA is used as a template to make protein
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RNA to Protein
• Ribosomes
• The code for individual genes gets copied to RNA
• RNA is used as a template to make protein
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mitochondrion
cytoplasmic fluid
flagellum
vesicle
ribosomes on rough ER
centriole
intermediate filaments (cytoskeleton)
Golgi apparatus
cytoplasm
lysosome
exocytosis of material from the cell
polyribosome
nuclear pore
basal body
nuclear envelope
chromatin (DNA) nucleolus
nucleus
plasma membrane
rough endoplasmic reticulum
free ribosome
smooth endoplasmic reticulum
microtubules (cytoskeleton)
microfilaments
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plasma
membrane
DNA in
nucleoid region
cytoplasm, with
ribosomes
Cell wall outside the
plasma membrane
Prokaryotic Cells
Fig 4.4
Capsule
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central vacuole
cytoplasmic fluid plastid
vesicle
lysosome
cytoplasm
plasmodesmata
cell wall plasma membrane
intermediate filaments (cytoskeleton)
free ribosome
ribosomes
nucleus
nucleolus
nuclear pore chromatin
nuclear envelope
smooth endoplasmic reticulum
rough endoplasmic reticulum
Golgi apparatus
chloroplast
mitochondrion
microtubules (cytoskeleton)
cell walls of adjoining plant cells
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Turgur pressure in plant cells cytoplasm
(a) Turgor pressure provides support
central vacuole
cell wall plasma membrane
When water is plentiful, it fills the central vacuole, pushes the cytoplasm against the cell wall, and helps maintain the cell's shape
When water is scarce, the central vacuole shrinks and the cell wall is unsupported
Deprived of the support from water, the plant wilts
Water pressure supports the leaves of this impatiens plant
(b) Loss of turgor pressure causes the plant to wilt