Biological Laboratory Safety
description
Transcript of Biological Laboratory Safety
Biological Laboratory Safety
REHSRutgers Environmental Health and Safety
REHS supports Rutgers University by providing comprehensive and professional health, safety and environmental services to the University community.Protect Rutgers employees, students and visitors.REHS ensures compliance with government regulations
Regulatory Agencies The safety standards and practices
described here have been developed and are regulated by government agencies including CDC (Center for Disease Control) NIH (National Institutes of Health)
Agenda Lab Safety Biohazards Biosafety Levels Biosafety
Cabinets
Lab Safety Safe Laboratory
Practices Lab coats Safety glasses Proper footwear Hair back No food or drink in
the laboratory
Biohazard An agent of
biological origin that can cause disease in humans Microorganism Toxin Allergen
Biosafety
The combined use of laboratory practices, laboratory facilities and safety equipment
to work with potentially infectious microorganisms.
Why use biosafety practices?
To protect: Workers/Students Products/Experimental results Environment/Laboratory classroom
Biosafety Levels BL-1: agents are not known to cause
disease BL-2: agents are associated with
human disease BL-3: agents are associated with
human disease and are potentially transmitted as aerosols
BL-4: agents of life threatening nature
Biosafety Level 1 (BL-1)Use BL-1 when working with: Well characterized agents Agents that are not known to cause disease in
health humans Agents that are of minimal hazard to lab
personnel and the environment
Examples of BL-1 Agents: E. coli JM109, DH5a Saccharomyces cerevisiae
Biosafety Level 1 (BL-1)
Standard Work Practices Use mechanical pipetting devices Wash hands frequently Minimize splashes and aerosols Decontaminate work surfaces daily Handle wastes properly Maintain insect and rodent control
program
Biosafety Level 1 (BL-1)Personal Protective
Equipment (PPE) Lab coat or apron Safety glasses or goggles Gloves as needed
Biosafety Level 2 (BL-2)Use BL-2 practices when working with: Agents of moderate potential hazard to
personnel and the environment
Examples of BL-2 agents: Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Retroviral vectors Human cells in cell culture
Biosafety Level 2 (BL-2) Adequate illumination Eyewash facility Negative air pressure Autoclave available Biological safety cabinet Lab must be separated from public
areas
Biosafety Level 2 (BL-2)
Standard Work Practices Use mechanical pipetting devices Wash hands frequently Minimize splashes and aerosols Decontaminate work surfaces daily Handle wastes properly Maintain insect and rodent control
program
Biosafety Level 2 (BL-2)
Special Practices Place used slides and coverslips in sharps
containers, never in any other receptacle. Sharps containers are:
Red in color Marked with the biohazard symbol Puncture resistant Leak proof
Biosafety Level 2 (BL-2)
Personal Protective Equipment (PPE) Lab coat or apron Safety glasses or goggles Gloves Biosafety cabinet
Aerosols or splashes Large volumes High concentrations
Biosafety Cabinets (BSCs) Provide product, personal and
environmental protection. Various classes of BSCs are available
Amount of air exhausted Amount of employee protection
Biosafety Cabinets (BSCs)
Biosafety Cabinets (BSCs) Sterile air is exhausted from BSCs
through a HEPA filter. HEPA filter can trap particles to 0.3 microns Chemical vapors and fumes pass through
HEPA filters
Biosafety Cabinets (BSCs)
Helpful Hints Enter straight into the cabinet,
avoid sweeping motions Don’t place materials on the grill Keep discard pan or bag inside the
cabinet Decon materials before removal
from the cabinet
Decontamination Sterilization: destroy all microbial
life, including spores Disinfection: destroy a majority of
microbial life, but not necessarily spores
Antiseptic: germistat used on skin to inhibit growth of microorganisms
Decontamination
Agent Selection Degree of killing required Nature of item to be treated Ease of use Safety Cost Type and amount of organism
Decontamination
Methods Heat: steam heat, dry heat,
incineration Chemical: bleach, ethanol,
hydrogen peroxide, ethylene oxide, paraformaldehyde
Radiation
Decontamination
Disinfectants do not replace standard microbiological practices and good hygiene!
Biological Waste
Types Cultures, stocks Sharps Pipettes, tips and weigh boats Other materials
Biological WasteBL-1 Waste Solids
Collect and package in clear autoclave bags Autoclave to sterilize Dispose of in regular trash dumpster (as long as no
chemical or radioactive contaminants are present) Liquids
Collect in containers with lids Autoclave or treat with 10% bleach to sterilize Dispose of down the drain (as long as no chemical
or radioactive contaminants are present)
Biological Waste
Sharps (needles, syringes, scalpel blades, slides, blood vials, pasteur pipettes)
Collect in approved sharps container
Autoclave to sterilize Dispose of in medical waste boxes
Biosafety and REHS Protocol Approval
REHS provisionally approves experimental protocols.
All protocols must be approved if they involve: recombinant DNA, non-recombinant human, animal or plant
pathogens, human cell culture, human tissue/blood experiments
Laboratory inspections RU Biosafety Committee provides final
approval to protocols