BIOLASETHE INNOVATIVE, RESEARCHED

AND PATENTED

IMMUNO-MODULATOR

FOR ALL MONOGASTRICS

BIOLASE

THE SYMBIOTIC

INTESTINAL MICROBIAL FLORA

(MONO GASTRIC)

GRAM POSITIVESSTREPTOCOCCUS

LACTOBACILLUS

BIFIDOBACTERIUM

EUBACTERIUM

PEPTO-STREPTOCOCCUS

GRAM NEGATIVESCLOSTRIDIUM

VEILLONELLA

ENTEROBACTERIACEE

YEASTS (less than 0.1%)

STREPTOCOCCUS faecium

LACTOBACILLUS acidophilus, fermentum, salivarius, animalis, reuteri

BIFIDOBACTERIUM Globosum, pseudologum, animalis, thermophilum, boum, magnum, pullorum, suis, cuniculi, choerium.

(Sneath P.H.A., et all. 1986)

INTESTINAL MICROBIAL FLORA

INTERACTION MICROORGANISM / SURFACE

INTERACTIONMICROORGANISM/ RESISTANCE

2 PRIMARY FUNCTIONS:

Interaction MICROORGANISM/

SURFACE

A. PROTEOLYTIC ACTION

B. UROLYTIC ACTION

Interaction MICROORGANISM/RESISTAN

CEA. ORGANIC ACID

PRODUCTION (Acetic and Lactic, mostly)

- Antibacterial effect- Lower ammonia absorption- Lower amines absorption

Interaction MICROORGANISM/RESISTAN

CEB. Immunological activation

C. Production of Vitamins and Enzymes

D. N-nitrosamines Degradation

E. Reduction of Ammonia and Hydrogen Sulphide.

F. Production of bactericidal substances(self regulation)

INTESTINAL ECO-SYSTEM

STABILITY BALANCE

LIVE LACTOBACILLI/STREPTOCOCCI ADMINISTRATION

• Sensitive to chemical & physical stress (heat, pH,…)

• Slow growth (6-8 hours)• BIO-TYPE

BIFIDOBACTERIUM ORAL ADMINISTRTATION IMPOSSIBLE:

ANAEROBES

LIVE YEASTS

Naturally:• Acid resistant• pH resistant• Antibiotic resistant

LIVE YEAST• LIVE ORGANISM

(ANIMAL)

- CONSUMES

- PRODUCES

CARBOHYDRATES / SUGARS

ALCOHOL – CARBON DIOXIDE(BREWERY) ( BAKERY)

DEAD YEAST

• BY-PRODUCT OF BREWERY INDUSTRY

• SOURCE OF PROTEIN

• ALL NUTRIENTS ALREADY USED UP

• DOSAGE 5-10 Kg/Ton

- DRIED-UP YEAST-

BIOLASE

• DOES NOT FERMENT• DOES NOT REPRODUCE• DOES NOT PRODUCE ALCOHOL• DOES NOT PRODUCE CARBON DIOXIDE

NO MORE ALIVE

THE CELL IS WHOLE• VITAMINS• ENZYMES• NUCLEOSIDES• NUCLEOTIDES

BIOLASE cells at electronic microscope

BIOLASE cells at electronic microscope

BIOLASE cells after spray-drying

BIOLASE BIO-ACTIVITY CAN BE MEASURED

CAPACITY TO MAKE A LACTOBACILLI&BIFIDA POPULATION GROW

MORE THAN A CONTROL GROUP

BIOLASE BIO-ACTIVITY: MIN. 4.5

IN VITRO BIO-ACTIVITY TEST (INCUBATION TIME: 8 hours – temperature:

37°C)

366

10

612

0 5 10 15

TH

LT

LY

LT+LY

TH+LT

SP

SP+LT

SP+LT+TH

GROWTH

BIOLASE CONTROL

31 2 4 5 6 70

10

20

30Growth curves of 2 lactobacilli coltures, incubated at 37°C. After 5 hours you have BIOLASE highest bioactivity , as expression of the ratio between the two coltures growth levels (measured as Optical Density)

INCUBATION (HOURS)

OPTIC

AL D

EN

SIT

Y

BIOLASE BIOACTIVITY

BifidaBIOLASE

cells“digesting”

Lactobacilli

a BIOLASE cell

“eating”

Bifida BIOLASE cells

“digesting”

Tadpoles

The one on the right has been fed BIOLASE

Lactobacilli Population (white) attacked by pathogenes (black)

BIOLASE Cells(brown) super feeding

Lactobacilli&Bifida

Lactobacilli&Bifida population has grown stronger and larger,

thanks to BIOLASE

BIOLASE is the ideal nutrient for

Lactobacilli&Bifida growth