Bioinformatics for Metabolomics and Fluxomics E’ ? ? ? ? ? ? A?C? E ABCD E v1v1 v2v2 v3v3 v4v4...
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Transcript of Bioinformatics for Metabolomics and Fluxomics E’ ? ? ? ? ? ? A?C? E ABCD E v1v1 v2v2 v3v3 v4v4...
![Page 1: Bioinformatics for Metabolomics and Fluxomics E’ ? ? ? ? ? ? A?C? E ABCD E v1v1 v2v2 v3v3 v4v4 v5v5 v6v6 v7v7 ABCD E RL 1; metabolite identification RL.](https://reader035.fdocuments.in/reader035/viewer/2022062620/551a74535503463e778b6120/html5/thumbnails/1.jpg)
Bioinformatics for Metabolomics and Fluxomics
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RL 1; metabolite identification
RL 1 & 2; pathway reconstruction
RL 2; metabolic flux analysis
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Metabolites and Metabolic Fluxes Play Key Roles in Organisms
First Example Application Domain : 200,000 metabolites in plants
Metabolomics: (large scale) measurements of metabolites and their levels
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Metabolites and Metabolic Fluxes Play Key Roles in Organisms
Second Example : metabolic flux analysis inmicro-organisms
Fluxomics: (large scale) measurements of metabolic fluxes
Metabolic flux analysis of E. coli strain grown in chemostat culture
2.32.3 2.32.30.61.0 0.61.0
1.71.3 1.71.3 0.20.3 0.20.3
0.10.3 0.10.3
2.01.9 2.01.9
0.20.3 0.20.3
4.24.1 4.24.1
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4.13.9 4.13.9
4.44.8 4.44.8
5.04.5 5.04.5
0.60.9 0.60.9
0.81.1 0.81.1
1.00.2 1.00.2
2.62.5 2.62.5
2.01.5 2.01.5
2.82.7 2.82.7
3.63.9 3.63.9
0.80.1 0.80.1
1.21.0 1.21.0
2.01.5 2.01.5
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Metabolites and Metabolic Fluxes Play Key Roles in Organisms
Third Example : Human and Animal Brain Neurotransmitter Cycling
Fluxomics: (large scale) measurements of metabolic fluxes
from: Metabolic Engineering (2004)
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Goals Project
develop bioinformatics methods for
• metabolite and pathway identification • quantification of metabolite levels and isotopic
composition • analysis of dynamic metabolic experiments • quantification of metabolic fluxes
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Two Connected Research Lines
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Expertise in the Netherlands BundledKey Participants
Roeland C.H.J. van Ham, Raoul J. Bino, Centre for BioSystems Genomics / Plant Research International, Wageningen
Wouter A. van Winden, Joseph J. Heijnen, Kluyver Centre / Delft University of Technology, Dept. of Biotechnology
Johannes H.G.M. van Beek, Centre for Medical Systems Biology / VU University medical centre, Amsterdam
Ivo H.M. van Stokkum, Centre for Medical Systems Biology / Applied Computer Science, Vrije Universiteit, Amsterdam
Further participants / consultants / collaborators :on the one hand computer science/database (Bakker/Kok, Bal), signal analysis (Verheijen, Van Ormondt/De Beer) and bioinformatics (a.o. Heringa) expertise.
On the other hand many scientists with metabolic research expertise and interests.
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RL1: Metabolite Identification
Develop platform for identification of metabolites from high-throughput metabolome data
algorithms for compound identification from (LC-) mass spectrometry and NMR spectroscopy
databases for raw and processed information; retrieving matching spectra of known chemical composition
standardized and automated procedure for metabolite identification, in particular from LC-MS/MS (liquid chromatography coupled to tandem MS)
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Metabolite Identification
Bino et al. New Phytologist (2005) 166 : 427–438
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Metabolite Identification
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RL2: Metabolic Flux Analysis
Develop platform for flux analysis, derived from stable isotope incorporation measured with NMR and mass spectrometry
a problem solving environment for simulation and analysis of metabolic flux models and experimental design
optimization algorithms for flux quantification new metabolic pathway modules
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100%
1-13C1-glucose
100% 13C2-ethanol
NH4+
S. cerevisiaeD=0.1 h-1
air
Rapid samplingofbiomass
0.003
LC-MS
Extraction of glycolytic, PPP, TCA intermediatesfrom biomass
m/z
13C-experiment for metabolic flux analysis in micro-organisms
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Detection of mass isotopomer fractions of glucose-6-phosphate with LC-MS
=M+0(g6p)
elution time
M+2(g6p)
=12C-atom=13C-atom
=M+2(g6p)
=M+1(g6p)
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Fit to NMR multiplets of the
4-carbon of glutamate from a
biopsy from porcine heart
Frequency (ppm)
Flux Quantification in vivo Animal Experiment
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acetyl-CoA
acetate
5 C
tra ns po rt
6 C
4 C
g lu tam a teasp a rta te
une n rich ed su bs tra te s
In Vivo Metabolic Rates Estimated from 13C NMR Spectrum
TCA cycle flux =7.7 ± 3.0 µmol/g/min
Anaplerosis 16 ± 12 %of TCA cycle flux
glutamate content24.6 µmol/g
Transport time29.8 ± 11.6 sec
58 ± 23 % acetyl CoAfrom infused acetate
Transamination 17.4 ± 6.0 µmol/g/min
TCAcycle
Flux Quantification in Vivo Animal Experiment
Oxygen consumption by NMR(µmol/min/g dry)
Oxy
gen
cons
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ion
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bloo
d ((
µm
ol/m
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dry
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0 5 10 15 20 25 30 35
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Myocardial Metabolism
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Integrated Problem Solving Environment
Integrated PSE (Problem Solving Environment) for metabolic flux experiment analysis
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Large Data Sets Analysed
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Summary
Bioinformatics tools and problem solving environ-ments are developed for
• metabolite identification and quantification• analysis of dynamic experiments and
quantification of metabolic fluxes• expertise in the Netherlands is bundled• collaboration of bioinformaticians, computer
scientists and domain experts
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