Bioinformatics
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Bioinformatics Lab Non-duplicate Unigenes Selection Protocol Large-scale EST assembly using phrap Contigs Unassembled ESTs Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs Rearray selected ESTs to 384-well plates Plasmid DNA preps 5’ sequencing 3’ sequencing PCR amplification of inserts Unigenes Sequence processing EST validation and duplicates removal Probe delivery Bioinformatics Lab
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Non-duplicate Unigenes Selection Protocol. Large-scale EST assembly using phrap. Contigs. Unassembled ESTs. Unigenes. Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs. Rearray selected ESTs to 384-well plates. - PowerPoint PPT Presentation
Transcript of Bioinformatics
Bioinformatics
Lab
Non-duplicate Unigenes Selection Protocol
Large-scale EST assembly using phrap
Contigs Unassembled ESTs
Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs
Rearray selected ESTs to 384-well plates
Plasmid DNA preps
5’ sequencing
3’ sequencing PCR amplification of inserts
Unigenes
Sequence processing
EST validation and duplicates removal
Probe delivery
Bioinformatics
Lab