BIOFILM DESTRUCTIVE SYSTEM Nkele Davis

Partners: Deborah Ohiani-Jegede, Nick Xydis

Biofilms

Created by free-floating bacteria that adhere to a surface

Form a complex and layered community with extracellular matrix

Biofilms then create more free-floating bacteria

Biofilm Resistance

Resistant to: antibiotics immune

response

Typical response to biofilm growth is to dispose of the infected product

Biofilms and Health Care

Formation on catheters (urinary, heart, respiratory, intravenous etc.)

Prosthetic valves, artificial hips, teeth, contact lenses

CDC reports biofilms are responsible for 65% of infections

Biofilms and Contact Lenses

Improper cleaning methods by users can lead to biofilms forming on contact lens cases

Rates of contamination on contact lens cases vary from 24% to 81%

Contact lens contamination can lead to microbial keratitis, conjunctivitis, staphylococci

Project Scope

Design a product that can destroy biofilms on contact lens cases

Focus on biofilms that cause microbial keratitis

Portable, handheld device Reasonable application time (<1hr) Biofilm inactivation rate of at least 95%

Existing Solutions

Case cleaning happens as a consequence of lens cleaning Heat Hydrogen Peroxide

solutions Multipurpose solutions

Silver impregnated cases to dissuade biofilm formation

Existing Solutions

Purilens Magnetic pedal

on a flexible arm is used to agitate the saline solution and loosen deposits from the lenses

UV light (253.7 nm) is used to disinfect the lenses, case, and saline solution

15 minute application period

Novelens Two chamber

case that keeps lens used solution (containing deposits) separate from fresh solution during the disinfection process to discourage biofilm formation

QuickPure Battery-powered

device which generates ozone as needed from the oxygen in air and bubbles it into barrel to clean and disinfect the lenses and the case

3 minute application period

Existing Solutions

Johnson & Johnson Vision Care

A patent for a lens storage case that uses disinfecting UV radiation to clean lenses and the case

The Compliance Case Co

A patent for a limited use, self destructive contact lens case

case leaks in a pre-determined fashion to alert user of end point of safe utilization

Design Specifications/Requirements

Usability Within 3” x 5” Weight: < 5 lb Operating time: < 1

hours Visual timer display Set up Time: < 30

seconds Sound during

application: < 40 dB Autonomous system,

turns on and off automatically

Waterproof

Economics Cost: < $200 (prescribed

by doctor) Power Draw: < .25kWhr

per application Effectiveness

95% biofilm destruction biofilm growth

prevention No damage to lens case

and lens Durability

meets all other specification requirements after 5 years

Preliminary Analysis

CFU = Colony Forming Unit

Power Draw: ~.25kW

The product will cost $9.03 per year Sound during application <40dB

Sound in a quiet room

Design Schedule

September 30th – Preliminary PresentationOctober 2nd – Preliminary Report Due 5th – Complete User Interviews 7th – Website Operational 9th – DesignSafe Report 11th – Brainstorm Application Types 13th – Brainstorm User Practices 18th – Evaluate/Improve Design Ideas 30th – Progress Presentation/Report

Design Schedule

November 2nd – Narrow Physical Design 8th – Contact Manufacturers 10th – CAD model of Design 15th – List of Materials 16th – Design Verification 22nd – Final Paper Draft 29th – Final Paper RevisionDecember 2nd – Final Presentation 4th – Final Report Due

Organization of Team

Nkele Consumer Liaison, Lead Ergonomics Designer

Deborah Materials Expert, Lead Mechanical Designer

Nick Time Management, Lead Electrical Designer

Shared Client Contact System Integration Informational Research Risk Management Website Upkeep

Questions??