Biodynes® 03 Bioactive (Patent Pending) Protect Skin Against Environmental Aggressors

Our modern urban environment is filled with chemicals and physical particles that have a harmful effect on our skin. One of those components that have been identified as potentially having a detrimental effect on skin is ozone (O3).1-9 Some of the effects ozone can have is to instantly react with important antioxidant reservoirs in the skin, such as vitamin C and vitamin E, and can convert essential skin lipids into lipid peroxidases which can ultimately lead to skin degradation. The resulting effect could lead to a loss of skin elasticity, premature aging, and overall lackluster skin.

Key Product Benefits – Protects from environmental aggressors – Reduces appearance of wrinkles and fine lines – Mitigates the visible effects of premature aging

Key Consumer Benefits – Lipid Barrier Improvement – Ozone Protection Factor™

Claim Ideas – Skin Smoothing and Firming – Environmental Protection – Urban Defense – Enhanced Barrier for Better Moisture Retention

INCI Name Saccharomyces Ferment Lysate Filtrate

Use Levels1.0–5.0 %

Suggested Formulations

– BB and CC Creams – Anti-Aging Serums – Moisturizing Essences – Daytime SPF Moisturizers

Personal Care

www.lonza.com www.lonza.com/personalcare

Personal Care – Biodynes® 03 – 03/16

USALonza Consumer Care70 Tyler PlaceSouth Plainfield, NJ 07080Tel +1 908 561 5200

SwitzerlandLonza LtdMuenchensteinerstrasse 384002 BaselTel +41 61 316 81 11

Review and follow all product safety instructions. All product information corresponds to Lonza’s knowledge on the subject at the date of publication, but Lonza makes no warranty as to its accuracy or completeness and Lonza assumes no obligation to update it. Product information is intended for use by recipients experienced and knowledgeable in the field, who are capable of and responsible for independently determining the suitability of ingredients for intended uses and to ensure their compli-ance with applicable law. Proper use of this information is the sole responsibility of the recipient. This information relates solely to the product as an ingredient. It may not be applicable, complete or suitable for the recipient’s finished product or application; therefore republication of such information or related statements is prohibited. Information provided by Lonza is not intended and should not be construed as a license to operate under or a recommendation to infringe any patent or other intellectual property right. No claims are made herein for any specific intermediate or end-use application.

© 2016 Lonza

In Vitro Efficacy Results

In Vitro Ozone Damage Protection Study

100

90

80

70

60

50

40

30

20

10

0

Treatment Percentage of Biodynes® 03

Perc

enta

ge o

f Pro

tect

ion

1 2 3 4 5

low

medium

high

Fig. 1

– MatTek™ full thickness tissue exposed to ozone (1.2 ppm) for 1 hour [equivalent to 8 hours exposure of skin on a highly polluted day]

– Biodynes® O3 bioactive effectively offers Ozone Damage Protec-tion against oxidative stress, as shown by decreasing levels of 8-oxoguanine

Dermatological Assessment of Facial Skin Firmness

80

70

60

50

40

30

20

10

0

% Im

prov

emen

t

5% Biodynes® O3

Placebo

3% Biodynes® O3

% Improvement in Skin Firmness

30 60 90

Time in Days

Fig. 2

– 90-Day Study conducted on 60 volunteers – 3% Biodynes® O3 bioactive offered a 61% improvement – 5% Biodynes® O3 bioactive showed a 72% improvement – Study conducted in São Paulo, Brazil during the summer months,

when ozone levels are at their highest

Dermatological Assessment of Overall Skin Appearance

Before: Panelist 1: 5% Biodynes® O3 After: Panelist 1: 5% Biodynes® O3

Fig. 3

After a 90-day treatment with 5% Biodynes® O3 bioactive, a statistically significant anti-wrinkle effect was observed in 34% of the participants compared to placebo-treated controls.

References1. Thiele JJ, Traber MG, Polefka TG, Cross CE, Packer L. OzoneExposure Depletes Vitamin E and Induces Lipid Peroxidation

in Murine Stratum Corneum. J Invest Dermatol 108; 753-757, 1997.2. Thiele JJ, Traber MG, Tsang K, Cross CE, Packer L. In Vivo Exposureto Ozone Depletes Vitamins C and E and Induces Lipid

Peroxidation in Epidermal Layers of Murine Skin. Free Rad Biol Med 23; 385-391, 1997.3. Weber SU, Jothi WS, Thiele JJ. High Pressure Liquid Chromatographic Analysis of Ozone-Induced Depletion of Hydrophilic

and Lipophilic Antioxidants in Murine Skin. Method Enzymol 319; 536-546, 2000.4. Weber SU, Han N, Packer L. Ozone: An Emerging Oxidative Stressor to Skin. Curr Probl Dermatol 29; 52-61, 2001.5. Cotovio J, Onno L, Justine P, Lamure S, Catroux P. Generationof Oxidative Stress in Human Cutaneous Models Following

In Vitro Ozone Exposure. Toxicol in Vitro 15; 357-362, 2001.6. Packer L, Valacchi G. Antioxidants and the Response of Skin toOxidative Stress: Vitamin E as a Key Indicator. Skin

Pharmacol Appl Skin Physiol 15; 282-290, 2002.7. Thiele JJ, Dreher F, Maibach HI, Packer L. Impact of Ultraviolet Radiation and Ozone on the Transepidermal Water Loss as

a Function of Skin Temperature in Hairless Mice. Skin Pharmacol Appl Skin Physiol 16; 283-290, 2003.8. Valacchi G, Pagnin E, Corbacho AM, Olano E, Davis PA, Packer L. In Vivo Ozone Exposure Induces Antioxidant/Stress

Related Responses in Murine Lung and Skin. Free Radic Biol Med 36; 673-681, 2004.9. Tavakkol A, Nabi Z, Cardona S, Soliman N, Polefka T. Tropospheric Ozone Induces Lipid Hydroperoxides and Depletes

Vitamin E in Human Skin In Vivo with no Detectable Clinical or Biophysical Changes. J Invest Dermatol 114; 844, 2000.