NAME : VIJENDREN KRISHNAN

SUPERVISOR : PROF. DR. YOSHIMITSU UEMURA

CO-SUPERVISOR : DR. NURLIDIA MANSOR

DATE : 22 SEPT 2014

BIODIESEL PRODUCTION BY

SIMULTANEOUS EXTRACTION AND

CONVERSION OF TOTAL LIPIDS FROM

MICROALGAE, CYANOBACTERIA, AND

WILD MIXED-CULTURES

PAPER REVIEW

Biodiesel: Renewable fuel produced from biological

oils.

Microalgae are known to accumulate 20-50%

triglycerides (DW).

Transesterification

Oil + Alcohol Fatty acid alkyl ester (FAME)

Catalyst (Acid/ Base)

INTRODUCTION

Type Strains Media Source

Marine microalgae Chaetoceros gracilis Artificial seawater UTEX*

Marine microalgae Phaedactylum tricornutum Artificial seawater UTEX*

Marine microalgae Tetraselmis suecica Artificial seawater UTEX*

Freshwater

microalgaeNeochloris oleoabundans Bristol Media UTEX*

Freshwater

microalgaeChlorella sorokiniana Bristol Media UTEX*

Cyanobacteria Synechocystis sp pcc 6803 BG -11 Hu et al., 2000

Cyanobacteria Synechocystis elongatus BG-11 Hu et al., 2000

EXPERIMENTAL STRAINS

* Culture collection of algae from the University of Texas, Austin.

Lipid extracted using organic solvent

Solvents removed by distillation

TG transesterified

CONVENTIONAL OIL

EXTRACTION

DIRECT TRANSESTERIFICATION

100mg lyophilized biomass

Reacted with MeOH + H2SO4

Reaction stopped by adding chloroform

Phase separation by adding water 5mL , centrifuged

Residual biomass recovered with 2mL chloroform

TOTAL LIPID ANALYSIS

TAG CONTENT DETERMINATION BY GAS

CHROMATOGRAPHY

FAME CONTENT ANALYSIS BY HAS

CHROMATOGRAPHY

ANALYSES

RESULTS AND DISCUSSION

RESULTS AND DISCUSSION

RESULTS AND DISCUSSION

Direct transesterification is applicable to

diatoms, green algae, cyanobacteria, and a

wild mixed culture

High biodiesel yield.

CONCLUSION

THE END