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BioDeep Meeting V: Marseilles, 13-15 th April 2002 (1 st report) Workpackage 5 : Microbial metabolic...
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BioDeep Meeting V: Marseilles, 13-15 th April 2002 (1 st report) Workpackage 5 : Microbial metabolic activities Armand Bianchi, Jean Garcin, Christian Tamburini Proof for the existence of bacterial life in extreme and selective environments (high pressure, anoxia, hypersalinity…) ep – WP5 – Marseilles, 13-15th April 2002
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Transcript of BioDeep Meeting V: Marseilles, 13-15 th April 2002 (1 st report) Workpackage 5 : Microbial metabolic...
BioDeep Meeting V: Marseilles, 13-15th April 2002 (1st report)Workpackage 5 : Microbial metabolic activities
Armand Bianchi, Jean Garcin, Christian Tamburini
Proof for the existence of bacterial life in extreme and selective environments (high
pressure, anoxia, hypersalinity…)
1 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Sampling?
Interface brine-sediments andsediments with multicorrer
Brines with Niskin and HP5L fitted on the Scipack
Seawater-Brine interface with Niskinfitted on the Scipack
Normal seawater with HPSS
2 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Bacterial production
Respiration(CO2)
prokariotic
Organic polymers
Uptake of compounds < 600 Da
organic monomers
Topics and measurements realized
ectoenzymatic activities
3 - BioDeep – WP5 – Marseilles, 13-15th April 2002
pmol L-1
0 5000 10000 15000
EAA
PA
Glutamate uptake (pmol L-1)
0
500
1000
1500
2000
2500
3000
3500
0 200 400 600 800
Dep
th (
m)
0 5 10
Bacterial production (ng C L-1)
GRBP
GA
References for ectoenzymatic activity, bacterial production and glutamic acid uptake in the whole
“normal” water column
GA: glutamic acid assimilationGR: glutamic acid respirationBP: bacterial biomassEAA: aminopeptidase activityPA: phosphatase activity
4 - BioDeep – WP5 – Marseilles, 13-15th April 2002
L’Atalante basin: an exemple of vertical profile of aminopeptidase rates
0.0 0.5 1.0 1.5 2.0 2.5 3.0nmol L-1 h-1
"Normal" sea-water3000m-depth
Seawater – brine interface
Body brine
Brine – sediment interface
0.0 0.5 1.0 1.5 2.0 2.5 3.0µmol L-1 h-1
Sediment 0-2 cm
Sediment 2-4 cm
5
Seawater – brine interface: differences between rates for the four basins
D: Discovery
A: L’Atalante
U: Urania
B: Bannock
DW: Deep-water
Aminopeptidase
pM h
-1
D A U B DW0
500
1000
1500
2000
2500
3000
3500
4000
Phosphatase
D A U B DW
Glutamate Assimilation
pM h
-1
Glutamate respiration Bacterial Production
ng C
L-1 h
-1D A U B DW D A U B DW D A U B DW
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0
5
10
15
20
25
30
35
40
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
n.d. n.d. n.d. n.d. n.d.
n.d.: not determined
6
Body brine: differences between rates for the four basins
Aminopeptidase
pM h
-1
Phosphatase
Glutamate Assimilation
pM h
-1
Glutamate respiration Bacterial Production
ng C
L-1 h
-1
D: Discovery
A: L’Atalante
U: Urania
B: Bannock
DW: Deep-water
D A U B DW D A U B DW
D A U B DW D A U B DW
0
200
400
600
800
1000
1200
1400
1600
0
1000
2000
3000
4000
5000
6000
7000
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
D A U B DW0
5
10
15
20
25
30
35
40
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
n.d. n.d. n.d.
n.d.: not determined
7
Body brine: differences between rates for the four basins
Aminopeptidase
pM h
-1
Phosphatase
D: Discovery
A: L’Atalante
U: Urania
B: Bannock
DW: Deep-water
D A U B DW D A U B DW0
200
400
600
800
1000
1200
1400
1600
0
1000
2000
3000
4000
5000
6000
7000
n.d.: not determined
Glutamate Assimilation
pM h
-1
Glutamate respiration Bacterial Production
ng C
L-1 h
-1
D A U B DW D A U B DW0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
D A U B DW0
5
10
15
20
25
30
35
40
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
n.d. n.d. n.d.
7
Brine – sediment interface : differences between rates for the four basins
Aminopeptidase Phosphatase
Glutamate respiration
n.d.
Glutamate Assimilation
n.d.
0
1000
2000
3000
4000
5000
6000
pM h
-1
n.d. n.d.
0
50
100
150
200
250
300
350
400
pM h
-1
1353522167
D: Discovery
A: L’Atalante
U: Urania
B: Bannock
SW: Sediments outside basinD A U B SW D A U B SW
D A U B SWD A U B SW
n.d.: not determined
8
Sediments: differences between rates for the four basins
Aminopeptidase Phosphatase
Glutamate respiration
0
20
40
60
80
100
120
140
µM
h-1
0
500
1000
1500
2000
2500
3000
3500
4000
nM h
-1D: Discovery
A: L’Atalante
U: Urania
B: Bannock
SW: Sediments outside brineD A U B SW D A U B SW
D A U B SW
l.d. l.d.
l.d.: lower than detectable
l.d.
DISCOVERY sediments collected with box correr
(Biodeep cruise – August 2000)
10 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Sediments: differences between rates for the four basins
Aminopeptidase Phosphatase
Glutamate respiration
0
20
40
60
80
100
120
140
µM
h-1
0
500
1000
1500
2000
2500
3000
3500
4000
nM h
-1D: Discovery
A: L’Atalante
U: Urania
B: Bannock
SW: Sediments outside brineD A U B SW D A U B SW
D A U B SW
l.d. l.d.
l.d.: lower than detectable
l.d.
9 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Are DHABs’ bacteria adapted to these extreme environmental conditions?
- HP: samples maintained in ambient condition(anoxia, high pressure, ionic equilibrium…)+ guarantee for aseptic condition
-DEC: samples decompressed (change in pressure, ionic equilibrium,anoxia?…)
10 - BioDeep – WP5 – Marseilles, 13-15th April 2002
High Pressure Bottle
Are DHABs’ bacteria adapted to these extreme environmental conditions?
Average = 10.3n = 13
Pe NSW
Average = 11,5n = 7
Pe brine
Pe =HP
DEC rates measured on samples decompressedrates measured in ambient condition
=11
DHABs EAA PA GA GR BP nMol h-1 nMol h-1 pMol C h-1 pMol C h-1 ng Cl-1 h-1
L'ATALANTE Brine
decompressed 0.07
Brine ambient pressure
0.12
BANNOCK Brine
decompressed 0.13 1.46 0.07 3.42 0.36
Brine ambient pressure
0.44 2.28 3.98 15.02 1.24
Conclusion Bacterial activities were detected within all niches sampled: seawater – brine interface, brine, brine – sediment interface and sediment of all DHABs
Distribution of studies activities within the different niches differ for each basin
Aminopeptidase activity was higher in brines comparatively to "normal" deep-sea water
Glutamic acid uptake in DHABs sediment was lower than in "normal" deep sediments
Brine-living bacteria appear to be adapted to these special ambient conditions (high pressure, high salinity, anoxia,…)
It’s the first demonstration of bacterial activities using an experimental strategy which avoided pressure, oxic, …, stresses during retrieval, incubation and sub-sampling.
12 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Discussion…
Interface or brine sampling?
12 - BioDeep – WP5 – Marseilles, 13-15th April 2002
Bacteria adapted? But also bacteria originated of the seawater column
We pointed out life and activities with bacteria adapted
We need result. Is it possible to have safe samples in interface like within the body brine?We have seen the difficulties to compare results obtained at the interface.Maybe, it is more reasonnable to intensify sampling within the bodin brine…
Thanks for your attention…
