BioConfirm B.08.00 Presentation - Agilent · BioConfirm B.08.00 Customer Presentation 5 BioConfirm...
Transcript of BioConfirm B.08.00 Presentation - Agilent · BioConfirm B.08.00 Customer Presentation 5 BioConfirm...
BioConfirm B.08.00 Presentation
17 June 2016
BioConfirm B.08.00 Customer Presentation
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Major Enhancements to Increase Your ProductivityThe Latest Version of our BioConfirm Product, B.08.00
BioConfirm is protein and peptide characterization software
using accurate mass LC/MS and MS/MS data from Agilent
instruments. Major new features are:
• Usability and user experience improvements
• New disulfide bond mapping and scrambling workflow
17 June 2016
BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 Offers the Lab Administrator
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BioConfirm B.08.00 out of the box increases productivity of your lab! No need to create multiple methods or to manage sequence
lists. Spend your time only reviewing samples that need your expert attention.
With Automatic mass range detection
• Never miss proteins ortheir modifications orimpurities
• No need to create aspecial method perBiomolecule or samplecondition.
Standalone Sequence Manager
• Organize your sequencesat one central database
• Cached preprocessingresults makes dataanalysis faster, increasesthroughput
Easy Batch view
• Find the sample that needsreview
• Red/ Green / Yellow flags
• Result review only modefor Biologist can restrictthem from changingmethod parameters
17 June 2016For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 Offers the Biologist
BioConfirm B.08.00 Customer Presentation
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Tell BioConfirm B.08.00 how you prepared your sample and collect your report! No need to understand Mass Spectrometry!
Answer 4 questions about
your sample
Press the green button
BioConfirm will tell you how
well the protein was confirmed,
along with reports and results
17 June 2016For Research Use Only. Not for use in diagnostic procedures.
Simple and Clean UI for both: Lab Admin and Biologist
BioConfirm B.08.00 Customer Presentation
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BioConfirm B.07.00 and previous versions were add on of MassHunter
Qual.
BioConfirm B.08.00 is a separate application which works side by
side with MassHunterQual.
There is also centralized Sequence Management by Sequence Manager
application.
17 June 2016For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 – Disulfide Bond Mapping
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Cysteine disulfide bonds
- assist in maintaining structure of proteins
- incorrect disulfide linkages can generate protein misfolding
- important to identify location of these bonds and confirm that
they are correct
BioConfirm B.08.00 workflow for:
- Disulfide bond mapping
- Disulfide bond scrambling
For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 – Disulfide Bond Mapping
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BioConfirm B.08.00 Customer Presentation
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16 disulfide bonds total
- 4 intra chain (light
chain)
- 8 intra chain (heavy
chain)
- 4 inter chain
Sample prep is very
important for this workflow
as it can cause non-native
scrambling of disulfide
bonds
For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 – Disulfide Bond Mapping
17 June 2016
BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 – Sample Comparison
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BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
BioConfirm B.08.00 – Deconvolution Mirror PlotAllows you to compare biosimilars or lot-to-lot variation
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BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
Sample-based Navigation
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BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
New BioConfirm tab in Acquisition Method Editor
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BioConfirm B.08.00 Customer Presentation
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For Research Use Only. Not for use in diagnostic procedures.
Disulfide Bond Mapping Application NoteP/N 5991-6951EN
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For Research Use Only. Not for use in diagnostic procedures.
ASMS 2016 Poster on Disulfide Bond Mapping
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BioConfirm B.08.00 PS Training June 2016
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For Research Use Only. Not for use in diagnostic procedures.
Summary
Agilent offers a complete workflow for the
characterization of intact proteins and peptide digests:
• Solid, reliable LC/MS hardware
• Dedicated consumables in the AdvanceBio columns line
• Integrated software from data acquisition through reporting
• Open access productivity with MassHunter Walkup that
allows new users to be trained in five minutes
New features have been added to BioConfirm B.08.00
for enhanced usability and disulfide bond mapping
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For Research Use Only. Not for use in diagnostic procedures.
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