Biochemistry preastation
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Transcript of Biochemistry preastation
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BIOSYNTHESIS OF LIGNIN
Course teacher :- Dr.uma ProfessorDr . Govindraju professor
Presented by :- Kale Ravindra
Ramrao
09-607-05
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INTRODUCTION• Lignin is a cross-linked phenolic polymer that interferes with
the degradation of plant cell walls.
• It find in vascular tissues cell wall as well as in cell walls of pith, root, fruit
.• Not uniformly distributed in call wall
• Protect microfibrils from chemical, physical , and biological attack.
• give hydroxycinnamyl alcohols or "monolignols", the monomeric precursors of lignin .
• Strengthens the cell wall
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Enzymes involved in lignin biosynthesis
• cinnamyl-alcohol dehydrogenase (CAD)• phenylalanine ammonia-lyase(PAL)• caffeoyl-CoA 3-O-methyltransferase
(CCOMT)• Cinnamate 4-hydroxylase (C₄H)• 4-coumarate CoA –ligase(4CL)• Ferulate-5-hydroxylase(F5H)• Cinnayml-coA reductase(CCR)
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• Oxidation of lignin with nitrobenzene in alkaline medium to find structure of lignin
• Gymnosperm lignin = vanillin + p-hydroxy benzaldihyde
• Dicot lignin = vanillin + syringaldehyde
• Monocot lignin = all three components
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In vitro synthesis of lignin like polymer
• Done by Fruedenerg in 1950 by using phenol oxidase enzyme which later on identified as laccase.
• Laccase carry out conversation of coniferyl alcohol to lignin like polymer.
• These were isolated and their structure determined.
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• Polymerization process involved the random non-enzymatic reaction of free radicals.
• Licot lignin is formed from coniferyl alcohols and sinapyl alcohols.
• Monocot lignin is formed from coniferyl, sinapyl and p-coumaryl alcohol
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• Symplastic cinnamylalochol glucosyltransferase storage (vacuole)
transportase apoplastic
cinnamyl glucosidesApoplastic cinnamyl alcohol β-glucosidase
lignin
Storage , transportation , and degeneration of Monolignol glucosids
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DISCUSSION
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THANK YOU