Bioactive lipids in milk of human and NZ dairy animals ... · Bioactive lipids in milk of human and...
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Bioactive lipids in milk of human and NZ dairy animals (cow, buffalo, sheep, goat). Kirill Lagutin, Andrew MacKenzie, Stephen Bloor, Dawn Scott and Mikhail Vyssotski
Transcript of Bioactive lipids in milk of human and NZ dairy animals ... · Bioactive lipids in milk of human and...
Bioactive lipids in milk of human and NZ dairy animals (cow,
buffalo, sheep, goat).Kirill Lagutin, Andrew MacKenzie, Stephen Bloor, Dawn Scott and Mikhail Vyssotski
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132 1
World milk production (Food and Agriculture Organization of the UN)
cattle
buffaloes
goats
sheep 0.1 0.33
26
39
0
5
10
15
20
25
30
35
40
45
Oceania Americas Europe Africa Asia
Milk from dairy species other than cow , %
water
fat
lactose
ashprotein
Dairy lipids (fat) analysis:
• Total lipids % (Fat content) – Extraction
• Fatty acids (AA, EPA, DHA, trans-FA) – Gas chromatography
• Phospholipids – 31P-NMR
• Cerebrosides – LC-MS/MS
• Gangliosides – Extraction & LC-MS/MS
• OPO analysis – RP-LC (preparative), Ag+-LC (analytical) Shimadzu LCMS-8040
Agilent GC 7890B
Fat. What is it made of?
Bruker 500 MHz NMR spectrometer
20.5 16.211.7
39.3
0.6 2.9
25.6
147.7
1.17.3
101.5
380.3
8.1 8.1
94.3
273.0
2.3 4.4
31.8
136.8
0.0
50.0
100.0
150.0
200.0
250.0
300.0
350.0
400.0
mg DHA per 100g milk mg AA per 100g milk mg CLA per 100g milk mg trans-FAs per 100g milk
Human milk Cow milk Buffalo milk Sheep milk Goat milk
3.6
4.0
6.5
5.6
3.2
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
Lipids, %
Human milk Cow milk
Buffalo milk Sheep milk
Goat milk
Fatty acids per 100g of milk
New EU regulation requirements for infant formulas (from 2020):• trans fatty acids <3% (of total fat) • erucic acid 22:1n-9 <1% (of total fat)• 18:2n-6 (500-1200mg per 100kcal)• 18:3n-3 (50-100mg per 100kcal)• DHA (20-50mg per 100kcal)• AA <1% (of total fat)• omega6 <2% (of total fat)• EPA ≤ DHA.
max
min
max
maxmax
max
max
max
Human milk Cow milk Buffalo milk Sheep milk Goat milk
10:0
10:0
10:0
4:0
14:0
12:0
3.6
4.0
6.5
5.6
3.2
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
Lipids, %
Human milk Cow milk
Buffalo milk Sheep milk
Goat milk
Medium chain triglycerides & fatty acids mg per 100g of milk
17.8
49.1
569.7
346.4
909.7
386.7
784.9
568.1
514.2
460.8
0.0
100.0
200.0
300.0
400.0
500.0
600.0
700.0
800.0
900.0
1000.0
Medium-chain triglycerides mg per100g milk Medium chain FA mg per 100g milk
Human milk Cow milk Buffalo milk Sheep milk Goat milk
caproic (C6:0)caprylic (C8:0)capric (C10:0)
TG 30:0
TG 30:0
3 MCFA
0 MCFA
1.4
8.7
5.9
10.1
14.6
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
16.0
Medium chain FA g per 100g fat
MCFA g per 100g of fat
Human milk Cow milk Buffalo milk Sheep milk Goat milk
3.6
4.0
6.5
5.6
3.2
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
Lipids, %
Human milk Cow milk
Buffalo milk Sheep milk
Goat milk
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
Humanmilk
Cowmilk
Buffalomilk
Sheepmilk
Goatmilk
PC
PI
PS
EPLAS
PE
SM
DHSM
0.0
10.0
20.0
30.0
40.0
50.0
60.0
mg PL per 100g milk0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
mg EPLAS per 100g milk mg SM per 100g milk
Human milk Cow milk Buffalo milk Sheep milk Goat milk
PhospholipidsNew EU regulation requirements for infant formulas <200mg
Human milk Cow milk Buffalo milk Sheep milk Goat milk
0.57
0.27
2.12
1.0
1.7
1.33 1.34
0.99
0.0
1.3
2.95
4.00
0.74
0.1
0.3
1.85
3.35
0.55
0.01 0.0
2.26
3.52
0.64
0.01 0.00.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
GlcCer mg/100g milk LacCer mg/100g milk GlcCer/LacCer GM3 mg/100g milk GD3 mg/100g milk
Human milk avg Cow milk Buffalo milk Sheep milk Goat milk avg
3.6
4.0
6.5
5.6
3.2
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
Lipids, %
Human milk Cow milk
Buffalo milk Sheep milk
Goat milk
Sphingolipids
!
Gangliosides (GM3, GD3) = GA
Human milk Cow milk Buffalo milk Sheep milk Goat milk
Have all the features
+
Gangliosides (!)
Medium-chain fatty acids
Phospholipids (including EPLAS & SM)
Cerebrosides
Phospholipids
DHA
Cerebrosides
Fat content
Conjugated linoleic acid
Cerebrosides
Gangliosides profile more human-like
Cow Buffalo Sheep Goat
OPO rich lipids (inFat) by AAK
IntegratedBioactive
Technologies
Biotechnology Capability
Fermentation Team
Lipids Team Natural Products Team
Protein Chemistry & Enzymes Team
Protein Science & Engineering Team
Process Engineering Team
Acknowledgements:
• Marita Broadhurst and Linda Samuelsson (AgResearch) – sheep milk samples
• Jaganmohan Billakanti (Callaghan Innovation) – goat, buffalo and human milk sample
• Mikhail Vyssotski (Callaghan Innovation) – cow milk sample
• Andrew MacKenzie (Callaghan Innovation) – goat milk sample
• Peter Dyer (Callaghan Innovation) – human milk sample
• Rashad Syed (Callaghan Innovation) – human milk sample
• Naomi Clements – human milk sample
