Bio-imprinting The advance of recent work
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Bio-imprinting The advance of recent
work
Mu yangyang
The ENZYME ENGINEERING
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The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
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Abstract
• Based on Vinyl Acetate
• Based on (+)-Ethyl Mandelate
Template : vinyl acetate + R-(-)-mandelic acid
a. Template : (+)-Ethyl Mandelate b. Template : (+)-Ethyl Mandelate + 1-phenylethanol
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Project
Template : Vinyl Acetate + R-(-)-Mandelic Acid
Conditions: Detecting per 2h
Results: No products were detected after 12h.
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a. Template : (+)-Ethyl Mandelate
Conditions: Detecting per 2h
Results: The promising results were abserved.
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Project
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a. Template : (+)-Ethyl Mandelate
Conditions: Detecting per 2h
Results: The promising results were abserved.
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Project
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Hot Tip
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Results: R-(+)-1-phenylethanol reduced obviously, while the S-(-)-1-phenylethanol barely reacted.
The conversion of the substrate was 17.2% and the eep>99%.
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Project
b. Template : (+)-Ethyl Mandelate + (R,S)-1-phenylethanol
Results: The results were also inspiring. The conversion of the substrate was 22.7% and the eep>99%.
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Comparison
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Template substrate
eep (%) Conversion (%)
EP
None 85 2.3 13(+)-ethyl
mandelate>99 17.2 1372
Imprinting with (+)-ethyl mandelate and 1-
Phenylethanol>99 22.7 5049
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The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
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Further work
To verify the hypothesis of bio-imprinting process in accordance with compulsory-order mechanism, the following work should be done.
Imprinted with R-(+)-1-phenylethanol
Imprinted with S-(-)-1-phenylethanol
Imprinted with Vinyl Acetate and R-(+)-1-phenylethanol / S-(-)-1-phenylethanol
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The ENZYME ENGINEERING
Contents
Recent developments1
Further plan2
Paper sharing3
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Paper
The STYLE of your expression in the paper was quite essential.
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The E values didn’t depend on the catalytic constants involved in the acylation step.
The sensitivity of the enantioselectivity was related to the enantiomeric form of the leaving alcohol, indicate that the imprinting effect involves modifications near the active site.
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Paper
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Asp and Glu with acidic residues were modified with EDC.
Chemical modification did not have a direct effect on chemical groups related to the enzyme activity, but may be altering the percentage or the shape of the open form of the lipase.
Combining the immobilization and amination seems to be a very useful tool in the combinatorial biocatalysis, which can change the characteristics of the enzyme like specific activity and enantioselectivity.
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