Biacore Molecular Interactions
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Transcript of Biacore Molecular Interactions
Biacore Molecular Interactions Shared Resource (BMISR)
Aykut Uren, MD, [email protected]
BMISR
• Have you ever wondered if two molecules directly interact with each other ?
• If they do, how strong is that interaction?
• Were you frustrated because you didn’t have the necessary technology or expertise to make it happen?
What can you use?
• Proteins
• DNA
• RNA
• Small molecules
What can you do?
DetectBinding Yes/No
IdentifySpecificity
Binding Partners
CharacterizeAffinityKinetics
ConcentrationThermodynamics
Biacore T-100
Integrated MicrofluidicsGold Sensor Chip
SPR Detection System
Biacore T-100
Real TimeSee binding as it happens
•Unique binding information (on- and off-rates) for any size of biomolecule•Study weak or fast interactions
Label Free•No labels required•Measure binding properties of unmodified substances •Reduce time and workload
Contract Free•Non-invasive•Measure opaque or colored samples
Sample Applications
(Chang)
Protein DNA interactions:• DNA repair enzymes binding to DNA• Transcription factors binding to DNA
Small Molecule Protein Interactions:• Small molecule inhibitors of enzymatic activities• Small molecule inhibitors of protein-protein interactions
Protein peptide interactions
Protein RNA interactions
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01
In your publications, remember to mention support provided from the GHUCCTS.