Bess Ward Department of Geosciences Princeton University.
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Transcript of Bess Ward Department of Geosciences Princeton University.
![Page 1: Bess Ward Department of Geosciences Princeton University.](https://reader036.fdocuments.in/reader036/viewer/2022062519/56649cb45503460f94977db2/html5/thumbnails/1.jpg)
Bess WardDepartment of Geosciences
Princeton University
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Weddell Sea
Ross Sea
Palmer Peninsula
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Pine Island Glacier breaks away from the Palmer Peninsula -- global warming at work?
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Cooling Trend in Antarctica -- warmer in polar seas
Doran et al., 2002
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Doran et al., 2002
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Weddell Sea
Ross Sea
Palmer Peninsula
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Doran et al., 2002
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Doran et al., 2002
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Antarctic Continental Cooling
Climate indicators Reduced T, decreased wind,
increased sunshine Reduced river flow, thicker ice
Ecosystem effects Less primary production Fewer nematodes
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Conflicting Signals of Climate Change in Antarctica
Indications of global scale change? Feedback effects of global climate? Ecological consequences?
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The Mystery of the Dysfunctional Nitrogen Cycle
Taylor Glacier
South Pole
West LB
East Lake Bonney
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West LB
East Lake Bonney
East Lake Bonney
STRANGE…
N cycle non functional:
Way too much nitrous oxide (N2O) and nitrate (NO3)
West Lake Bonney
NORMAL
N cycle functional:
Very little nitrous oxide (N2O) and nitrate (NO3)
One lake, two lobes connected at the surface
WHY????WHY????
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N2O = Greenhouse gas
UV radiation
Ozone layer
N2O involved in Catalytic ozone destruction
NO3
bacteria
Nitrous oxide (N2O):
intermediate in denitrification
N2
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Nitrogen Concentrations (µM)
NORMAL
Dep
th (
m)
West Lobe
ammonium
nitrate
East Lobe
STRANGE
ammonium
nitrate
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Nitrogen Concentrations (µM)
East Lobe
STRANGE
ammonium
nitrate
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Nitrogen Concentrations (µM)
East Lobe
STRANGE
ammonium
nitrate
(Priscu et al., 1996; LTER)
nitrous oxide
Dep
th (
m)
East Lobe
REALLY STRANGE
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First, drill a hole….
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Weather port, West Lobe Lake Bonney
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Sampling Hole through the Ice Cover
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“Lab” inside the weather port
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Isolate denitrifying bacteria and detect them in the lake
Species-specific immunofluorescence
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Distribution of strain ELB17 (Marinobacter) in Lake Bonney
(counted by immunofluorescence) East Lobe
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Optimal growth conditions for strain ELB17
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Nitrite reductase (nirS) genes in Lake Bonney
Central enzyme in denitrificaiton encoded by nirS
nirS genes found in both lobes
Paracoccus pantotrophusParacoccus denitrificans
Roseobacter denitrificans
NogalesAJ440476NogalesAJ440483
Ralstonia eutrophaNogalesAJ440492
NogalesAJ440487Alcaligenes faecalis
Pseudomonas stutzeri 14405Pseudomonas stutzeri JM300
Pseudomonas fluorescens
Pseudomonas aeruginosa[WLB16-24]
NogalesAJ440469NogalesAJ440478
NogalesAJ440471
NogalesAJ440473NogalesAJ440480
NogalesAJ440486NogalesAJ440475
NogalesAJ440489NogalesAJ440470
Braker pA12Braker pB66Braker pA4
Braker pB46Braker pC56
Braker pA90Braker pA29Braker pB76
Braker pA32Braker pA25
Braker pA98Braker pA94
Braker pB4Braker pB49Braker pA50
Braker pB20[WLB16-29]
Braker pA5Braker wF16
Braker wA15
NogalesAJ440477[ELB16-20][ELB16-35][ELB19-20][ELB19-1][ELB19-28][ELB19-3][ELB16-30][ELB16-33][ELB16-40][ELB19-38][ELB16-16][ELB19-24][ELB19-6][ELB19-36][ELB19-30][ELB16-32][ELB16-24][ELB19-16][ELB19-40][ELB19-25][ELB16-25][ELB16-9][ELB19-11][ELB16-4]
[ELB19-14][ELB19-15]
[ELB16-7][ELB16-28][ELB16-27][ELB16-26][ELB16-5]
[ELB19-33][ELB19-8][ELB19-4][ELB19-17][ELB19-35][ELB16-36][ELB16-31]
[ELB19-32][ELB19-26]
[ELB19-39][ELB16-39][ELB16-6][ELB19-18][ELB19-5][ELB16-34][ELB16-38][ELB16-11][ELB16-1][ELB19-42]
Halomonas variablis B9-12[ELB25-5]Marinobacter sp. C10-1
Marinobacter sp. D4-14[WLB16-4]
[WLB16-94][WLB16-58][WLB16-5]
[WLB16-35][WLB16-53][ELB25-43][WLB16-51][WLB16-74]
[WLB16-90][WLB16-93][WLB16-30][WLB16-40][WLB16-47]
[ELB25-7][ELB25-75][ELB25-19]
[ELB25-14][ELB25-55]
[ELB25-9][WLB16-10][WLB16-89][ELB25-56][ELB25-50][WLB16-7][WLB16-1][ELB25-78][WLB16-16][WLB16-82][WLB16-73][ELB25-31]
[WLB16-70][ELB25-59]
[ELB25-23][ELB25-41][ELB25-27][ELB25-39][ELB25-3][ELB25-2][ELB25-52][ELB25-76][ELB25-11][ELB25-62][ELB25-74][ELB25-18][ELB25-57][ELB25-79][ELB25-10][ELB25-13][ELB25-84][ELB25-34]
5%
100
10084
95
88
100
100
72
10062
70
100
85
78
62 100
100
77
66
86
95
99
96
99
72
62
93
100
64
75
90
63
100
91
90
Chris Francis
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Sharon Harris: Teacher Experiencing Antarctica
Measuring denitrification rates based on N2O production
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Dep
th (
m)
West Lobe
Denitrification rate
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Dep
th (
m)
East Lobe
Denitrification rate
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Denitrification does not occur in the East Lobe…but all the
ingredients are present
Denitrifiers can be isolated from the lake The strains survive under in situ conditions Denitrifying cells are detected in the lake Denitrifying genes are detected in the lake Denitrification happens in the bags, not in the
lake
So what is the #?@@*#&^&*$ problem??
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Nitrogen Concentrations (µM)
East Lobe
STRANGE
ammonium
nitrate
(Priscu et al., 1996; LTER)
nitrous oxide
Dep
th (
m)
East Lobe
REALLY STRANGE
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East West
Copper
low hi
SilverSilver hi low
Mark Wells
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Nitrite Reductase, Nitrous Oxide Reductase
NO3 NO2 NO N2O N2
Inducible, conserved, gas metabolism
(Cu, heme) (Cu)
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The Copper Hypothesis
CuCu is required for denitrification AgAg substitutes for CuCu in denitrification
enzymes, and makes them nonfunctional
AgAg toxicity or competition with CuCu inhibits denitrification in the East
But how did the two lobes end up with such different AgAg / CuCu ratios?
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LTEREast Lobe
West Lobe
Taylor Glacier
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Blood Falls, West Lake Bonney
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Blood Falls, West Lake Bonney
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Cross section of Lake Bonney today
W E
Taylor Glacier
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Lake Bonney 3000 years ago
“cold-dry” period
W E
Taylor Glacier
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W E
Taylor Glacier
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W E
Taylor Glacier
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W E
Taylor Glacier
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W E
Taylor Glacier
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Lake Bonney 1200 years ago
Hypersaline pool in the East Lobe
W E
Taylor Glacier
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W E
Taylor Glacier
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W E
Taylor Glacier
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Cross section of Lake Bonney today
W E
Taylor Glacier
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Geochemical Evidence
West and East Lobes have different histories
History reflected in chemical composition of the water
Modern biogeochemistry depends on past climate change
Future climate change depends on current biogeochemistry
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Bonney Hoppers
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Sunrise at McMurdo, 11 July 2001
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