Beer Haze and Colloidal Stabilization MBA… · Beer Haze and Colloidal Stabilization! Karl J....
Transcript of Beer Haze and Colloidal Stabilization MBA… · Beer Haze and Colloidal Stabilization! Karl J....
Beer Haze and Colloidal Stabilization!
Karl J. Siebert!
Food Science !& !
CORNELL UNIVERSITY GENEVA!Technology!
The Phenomenon of Haze!When insoluble particles are suspended in a liquid they scatter light, which makes a sample appear turbid or hazy.!Colloidal particle suspensions are indefinitely stable because collisions with the solvent molecules (caused by Brownian motion) keep the particles in suspension. !Larger or less buoyant particles tend to settle out.!
light source!
filter or mono-
chromator!
sample cell!
light detector!
Photometer!
With a photometer, some light that is scattered (as well as that absorbed) fails to reach the detector and results in reduced transmission. !
light source!
filter or lens! sample
cell!
light detector!
Turbidimeter!A turbidimeter responds to light that is scattered or deflected by interaction with particles. Turbidimeter detectors often are placed either at a narrow angle (typically 11 - 25º) or at a 90º angle to the incident light beam.!
Light absorption (in the visible range) makes samples appear colored.!
Light scattering makes samples appear cloudy or turbid.!
Haze Perception!The unaided human eye cannot actually see colloidal size particles, but can readily detect scattered light.!Particles scatter light at various angles depending on the wavelength of the light and the size and shape of the particle. !
There are quite a few reports in the brewing literature stating that narrow angle scattering is more sensitive for large particles (such as yeast, which has a mean particle diameter on the order of 10 µm) and that 90º scattering is more sensitive for small particles, such as chill haze (generally thought to be on the order of 0.25 µm or smaller).!Gales (JASBC 2000) pointed out that this is actually not correct, and is an artifact due to size differences between particles used for calibration and those actually present.!
Sources of Haze and Sediment in Beer!Foreign matter!
!adsorbents!!filter aids!
Crystalline material!!oxalates!
Microbes!!yeast!!bacteria!
Polysaccharides!!starch, pentosan, β-glucan!
Protein-Polyphenol complexes!
Foreign matter is normally only seen when process failures occur.!
That is also true for oxalate hazes (assuming calcium addition is done normally).!
Hazes produced by bacterial contamination (either by direct scattering or through formation of haze as a result of bacterial metabolism) are typically seen only if filtration or pasteurization fails for some reason.!
Yeast cell wall fragments arise when yeast is subjected to a shearing force (in a disc centrifuge or during agitation). !Disc centrifugation was shown to produce yeast cell wall fragments under some conditions; these were particularly resistant to sedimentation and harmful to filterability (Siebert et al., 1987). !Haze material was released from agitated yeast; this occurred to a greater extent at pH 2 or 8 than at pH 4 (Lewis & Poerwantaro, 1991).!Both the haze and the pH of a yeast suspension in beer increased slightly with increasing sheer severity (Stoupis & Stewart, 2003).!
Carbohydrate Hazes!Relatively pure carbohydrate hazes in beer are known (starch crystals, pentosans, etc.) but infrequently encountered.!Hazes isolated from beer are typically found to contain 70% - 80% carbohydrate, about 20% protein and a small amount of polyphenol (Belleau & Dadic, 1980, 1981; Siebert et al. 1981). However, it is well known that stabilization can be achieved by removing polyphenol or protein or both. So the carbohydrate must simply be entrained when the protein-polyphenol haze particles form.!
Main Haze Formation Mechanism!
Hazes in beer can occur from a number of causes, but most often arise from protein-polyphenol interaction. !
protein !+ !
polyphenols!
protein-polyphenol!complexes!
haze!
sediment!
Sensitive (haze-active) !Protein Test!
Tannic acid solution!
hold!
beverage sample!
polyphenol- protein haze !
measure light
scattering!
Thompson & Forward, J. Inst. Brew. 75: 37-42, 1969.!
Haze Forming Capacity of Various Proteins and Peptides Combined with Catechin and Heated!
Data from: Asano et al. J. Amer. Soc. Brew. Chem. 40: 147-154, 1982.!
B B
B
B B
B
B
B 0!
20!
40!
60!
80!
100!
120!
0! 20! 40! 60! 80! 100!
Haz
e-Fo
rmin
g C
apac
ity!
Mole % Proline!
We know from Asano’s work (JASBC 1982) that the beer haze-active protein is derived from barley hordein.!
Gliadin is the wheat protein that is analogous to barley hordein.!
Both gliadin and hordein are prolamins (alcohol-soluble, proline-rich proteins).!
Both have high contents of the amino acids proline and glutamine.!
Gliadin is commercially available.!
Amino Acid Sequence of Barley Hordein!(haze active protein in beer)!
Partial Sequence of Barley Hordein!Q Q Q P F P Q Q P I P Q Q P Q P Y P Q- Q P Q P Y P Q Q P F P P Q Q P F P Q Q- P V P Q Q P Q P Y P Q Q P F P P Q Q P- F P Q Q P P F W Q Q K P F P Q Q P P F- G L Q Q P I L S Q Q Q P C T P Q Q T P L- P Q - !
P = proline Q = glutamine!
-100!
-75!
-50!
-25!
0!
0! 1! 2! 3! 4! 5!
Free
Ene
rgy
(kJ
/mol
)!
ligand molality (10!2! mol / kg)!
O!H!O!H!
O!H!
O!H!
O!H!O!H!O!H!
Polyphenol Association with BSA at pH 6.5!
McManus et al., J. Chem. Soc. Perkin Trans. II: 1429-1438, 1985.!
O!
O!H!O!H!
O!H!
H!O!
O!H!catechin!
O!
O!H!O!H!
O!H!
H!O!
O!H!epicatechin!
O!
O!H!O!H!
O!H!
H!O!
O!H!OH!
gallocatechin!
O!
O!H!O!H!
O!H!
H!O!
O!H!O!H!
epigallocatechin!
Proanthocyanidin Building Blocks!
B!
B!
B!
0!
4!
8!
12!
16!
0! 1! 2! 3! 4!
Haz
e (E
BC)!
Degree of Polymerization!
monomer!
dimer!
trimer!
Effect of Proanthocyanidin (Catechin) Polymerization!
Mulkay & Jerumanis, Cerevisia 8: 29-35, 1983.!
Prominent Beer Proanthocyanidin “Dimers”!
Prodelphinidin B3! Procyanidin B3!
O!HO!
OH!OH!
OH!OH!
OH!OH!
OH!OH!
HO! O!
OH! O!HO!
OH!OH!
OH!OH!
OH!OH!
OH!OH!
HO! O!
McMurrough et al., J. Am. Soc. Brew. Chem. 54: 141-148, 1996!
B!
B!B!
B!
B!
B!B!B!B!B!B!B!B!J!J! J!
J!
J!
J!J!J!J!J!J!J!J!0!
1!
2!
3!
0! 1! 2! 3! 4! 5! 6! 7! 8!
Chi
ll H
aze
(EBC
)!
Added to Over-Stabilized Lager (mg/L)!
Prodelphinidin B3!
Procyanidin B3!
Effect of Dimeric Proanthocyanidins on Beer Haze!
C!
HO!OH!
OH!
O!
C!
O!OH!
OH!
O!O!
C!
OH!
OH!
O! O!
C!HO!
HO!
O! C!
OH!
O!
O!O!
OH!H!
H!2!C!
O!O!
H!H!
H!
H!
OH!
O!
O!
HO!OH!
C!
O!
HO! O!
OH!
C!
OH!
O!
HO!OH!
OH!
C!
C!
HO!
HO!
HO!O!
O!
One form of tannic acid!
Gallic acid!
Gallotannins!
H!O!O!H!H!O!
H!O! O!
Because protein-polyphenol haze can be induced by cooling and dispelled by warming we know it is not caused by covalent bonding.!
That leaves non-covalent interactions, which could be due to hydrogen bonding, hydrophobic bonding or ionic bonding. !
Expected Effects of Added Substances!N,N-dimethyl formamide (DMF) - a hydrogen bond
acceptor; this should compete with and reduce hydrogen bonding between proteins and polyphenols.!
!!Dioxane - a non-polar, but water miscible solvent; this
should compete with and reduce hydrophobic bonding between proteins and polyphenols.!
!!NaCl solution - highly ionic; this should compete with and
reduce ionic bonding between proteins and polyphenols.!
N
O
O
O
Added: !Haze Inhibition!No addition !-!25% DMF !+!25% Dioxane !+!5% NaCl !-!
Asano et al. JASBC 40: 147-154, 1982.!
Conclusion: protein-polyphenol interaction involves hydrogen and hydrophobic bonding, but not ionic bonding. !
Data from Siebert et al. J. Agric. Food Chem. 44: 80-85, 1996.!
The indicated substance was added to pre-formed gliadin-catechin in haze in buffer!
J!J!
J!
J!
B!B! B!
B!H!
H!H! H!
F!
F! F! F!0!100!200!300!400!500!
0! 5! 10! 15! 20!
Turb
idity
(NTU
)!
% Added!
J!20% NaCl!B!water!H!dioxane!F!DMF!
proportional to dilution!
Oh et al. (J. Agric. Food Chem. 28: 394-398, 1 9 8 0 ) c a r r i e d o u t m o d e l s y s t e m experiments in which tannins were combined with either gelatin or polyproline. !
They found that if either ionic strength or temperature increased, the haze increased. They pointed out that both phenomena are characteristic features of hydrophobic bonding.!
Work with buffer model systems showed that protein concentration (gliadin), polyphenol concentration (tannic acid), pH, and alcohol all affect haze intensity.!
A statistical experiment design was used to collect data that was used to construct a response surface model. !
!Siebert et al., J. Agric. Food Chem. 44: 1997-2005, 1996!
Behavior of Gliadin - Tannic Acid Model System!
100
200300
400500
20
40
6080
100
20
40
60
80
100
120
140
160
Tannic acid (mg/L)
Haze(NTU)
Gliadin (mg/L)
Siebert et al., J. Agric. Food Chem. 44: 1997-2005, 1996!
Polyphenol ≈ Protein!
Polyphenol >> Protein!Polyphenol << Protein!
Concept of Protein-Polyphenol Interactions!
Polyphenol molecule!
Protein molecule with fixed!number of phenol binding sites!
Siebert et al., J. Agric. Food Chem. 44: 80-85, 1996.!
Laser Diffraction Particle Size Analyzer!
laser! Photodiode arrays!
lenses!sample!
high angle scatter!
low angle scatter!
computer!
Particle size distribution plots for 40 mg/L TA at pH 4.5!
0.1! 0.618! 3.816! 23.58! 145.6!0!2!4!6!8!
10!12!14!16!
Num
ber %!
Particle diameter (µm)!
100!200!300!400!500!
Gliadin (mg/L)!
0.43!
2.7!
Siebert & Lynn, J. Amer. Soc. Brew. Chem. 58: 117-123, 2000.!
Particle size distribution plots for 200 mg/L Gliadin at pH 4.5!
0.1! 0.618! 3.816! 23.58! 145.6!0!2!4!6!8!
10!12!14!16!18!
Num
ber %!
Particle diameter (µm)!
40!60!80!100!120!140!
TA (mg/L)!
2.7!
0.41!
0.17!
Siebert & Lynn, J. Amer. Soc. Brew. Chem. 58: 117-123, 2000.!
Basically the results confirmed the mechanistic concept proposed earlier. The results showed differences in particle size with different gliadin/TA proportions. Larger particles were seen with intermediate ratios of protein/ polyphenol than with either lower or higher ratios.!
The particle sizes are amazingly quantized. Instead of gradual shifts of a monomodal distribution, particles of distinctly different particle size were seen under different conditions.!
Behavior of Gliadin - Tannic Acid Model System!
0246810
123.0
3.5
4.0
4.5
20
40
60
80
100
120H
aze
(N
TU
)
alcohol(%v/v) pH
Siebert et al., J. Agric. Food Chem. 44: 1997-2005, 1996!
haze (NTU)!
The effect of pH on haze intensity is striking. About 7 times as much haze was produced with the same amounts of protein and polyphenol when pH increased from 3 to slightly above 4. Further increases in pH resulted in declines in haze intensity. !!Additional studies showed the decline continued until at least pH 6.5.!
Sensitive (haze-active) !Protein Test!
Tannic acid solution!
hold!
Sample with haze-active
protein!polyphenol- protein haze !
measure light
scattering!
Haze (NTU) Produced by Addition of Various Amounts of Tannic Acid (TA) to Beverages!
! !!0.00 !16 !0 !56 !4 !2!!0.50 !18 !2 !62 !2357 !1811!!1.25 !19 !2 !68 !4174 !3393!!2.50 !23 !3 !71 !5302 !4244!
! ! Apple ! Apple Unstab.!!TA (g/L) !Juice 1 !Juice 2!Juice !Beer 1 !Beer 2!
Siebert et al., J. Agric. Food Chem. 44: 1997-2005, 1996!
So beer has a lot of haze-active (HA) protein while apple (and most other fruit) juice has very little.!
Grape juice and wine also have very little.!
Concept of Haze-Sensitive !Polyphenol Test!
haze-active peptide!solution!
hold!
sample with haze-active polyphenol!
haze-active peptide -
polyphenol haze!
measure light scattering !
Haze (NTU) Produced by Addition of Various Amounts of Gelatin to Beverages!
! !!0 !17 !2 !93 !4 !2!
!100 !254 !62 !168 !5 !2!!200 !307 !24 !243 !5 !2!!400 !329 !11 !289 !5 !3!
!gelatin ! Apple ! Apple ! Unstab. ! !!(mg/L) !Juice 1 !Juice 2! Juice! Beer 1 Beer 2!
Siebert et al., J. Agric. Food Chem. 44: 1997-2005, 1996!
Time Course of Protein-Polyphenol Haze Development !
Siebert, J. Agric. Food Chem. 47: 353-362, 1999!
J!J!J!J! J!
J!
J!
J!
0!20!40!60!80!
100!120!140!
0! 20! 40! 60! 80!100!120!
Haz
e (N
TU)!
Time (days)!
McMurrough et al., J. Amer. Soc. Brew. Chem. 50: 67-76,1992.!
Haze Development in Beer Treated With PVPP/SHG!
J! J! J!J! J!
J!J! J!
J!J!J! J!
J!
B! B! B! B! B!B! B!
B!B! B!
B!B!B!
H! H! H! H! H! H! H! H!H! H! H!
F!F!F!F!F!F!F!F!F!F! F! F! F!0!
1!
2!
3!
4!
5!
0! 3! 6! 9! 12! 15! 18!
Tota
l Haz
e (E
BC)!
Storage time at 37°C (weeks)!
untreated!
1X PVPP!
2X PVPP!
2X PVPP + 2X SHG!
McMurrough et al. J. Amer. Soc. Brew. Chem. 50: 67-76, 1992.!
Haze Development Model for Beer!
B
B
B
B
B B B B B 0!
0.1!
0.2!
0.3!
0.4!
0.5!
0! 5! 10! 15! 20! 25!
Haz
e Fo
rmat
ion
Rat
e !
Sensitive Proteins x Proantho. dimers!
Implications of relationship!Δ(haze)/time = [HA protein]·[dimeric proanthos]!
Stabilization can be achieved by reducing HA protein, HA polyphenol (dimeric proanthocyan-idins), or both.!Reducing HA protein by 50% should be equal in effect to reducing HA polyphenol by an equal percentage.!
Beer is usually stabilized in some way to delay the onset of protein-polyphenol haze formation beyond the intended product shelf-life.!
Methods of Colloidal Stabilization!Proteolytic Enzymes!
Papain, bromelin, etc.!Proline specific protease!
Cold Sedimentation!Fining agents!
Proteins (gelatin, isinglass)!Tannins (tannic acid)!
Adsorbents!Protein adsorbents (bentonite, silica gels)!Polyphenol adsorbents (polyvinylpolypyrrolidone)!
Withdrawing energy from a system by chilling, either during maturation or in the package, can result in greater precipitation of larger and denser particles.!It can also result in loss of solubility of some marginally soluble material, forming more colloidal matter.!During maturation the prolonged chilling tends to favor precipitation of particles. This leads to turbidity decreases and filterability increases. Both are related to removal of colloidal particles through sedimentation.!
B!B!
B
B!B!B!B!B! B!J!J
J J!
J!J!J!
J!J!
J!
0!
100!
200!
300!
400!
0!
50!
100!
150!
200!
250!
0! 2! 4! 6! 8! 10!12!14!
Haz
e (n
ephe
los)!
Filte
rabi
lity
(g)!
Storage Time (days)!
Siebert et al., MBAA TQ 24: 1-8, 1987.!
Ideally, brewers would like to settle colloidal material out during cold maturation.!This spares the load on filters, leads to more efficient operation and longer filter runs.!However, this requires long times at low temperatures, which is uneconomical.!Fining agents can speed up this process. Fining agents are generally either haze-active (HA) proteins (gelatin, isinglass) or HA polyphenols (tannic acid).!
Foam is an important property of beer that, like haze, involves protein. The protein involved in foam has a different composition than that involved in haze.!
However, methods of stabilization that non-selectively remove protein are destructive to foam and so unacceptable.!
It has been observed that the response of a commonly used protein assay (Bradford Method, based on Coomassie blue dye binding) gives a response in beer that correlates with foam performance. !
Siebert & Knudson, MBAA Tech. Quart. 26: 139-146, 1989.!
Hea
d R
eten
tion
Valu
e (R
udin
)!
High Molecular Weight Protein (Bradford Coomassie Blue)!6!0! 8!0! 1!0!0! 1!2!0! 1!4!0! 1!6!0! 1!8!0! 2!0!0!
1!1!5!
1!1!0!
1!0!5!
1!0!0!
9!5!
9!0!
8!5!
8!0!
F
F
F F F
F F F
F
F
F F
F
F F
F
F
F F
F F F F F F
F
F
F F F F F
F F
F F
F F F F F F F F
F F F F
F F F F F F F F F F F F
AMINO ACID!AMINO ACID!ARG!TYR!TRY!HIS!PHE!LYS!
0!
1!
2!
3!
4!
5!
MALT HORDEIN!
% O
F TO
TAL!
0!
1!
2!
3!
4!
5!MALT ALBUMIN &
GLOBULIN!
ARG! TRY!HIS!PHE!LYS!TYR!
% O
F TO
TAL!
0!
1!
2!
3!
4!
5!BEER FOAMING
PROTEINS!
% O
F TO
TAL!
COOMASSIE BLUE RESPONSE TO
HOMOPOLYMERS!Compton & Jones, 1985!
40!
0!
10!
20!
30!
ABSO
RBA
NC
E!
In beer, Coomassie blue dye binding (as used in the Bradford method for protein measurement) virtually ignores the large amount of haze-active hordein and responds to the smaller amount of foam-active albumins and globulins.!
Coomassie blue can then be used as a direct assay of foam-active protein in beer.!
!Siebert & Knudson, The relationship of beer high molecular
weight protein and foam, MBAA Tech. Quart. 26: 139-146, 1989.!
Unchillproofed beer was treated with different amounts of each of several adsorbents (bentonite, silica, PVPP).!
The haze-active protein, foam-active protein (by Coomassie blue dye binding) and haze-active polyphenol levels were estimated.!
The Effects of Bentonite on Haze-Active (HA) and Foam Active (FA) Beer Constituents!
FA protein (B)!HA protein (F)!HA polyphe (J)!
B!
B!B!
B!B!
J!
J!J!
J!
J!
F!
F!F!
F!F!0!
40!
80!
120!
160!
0!
20!
40!
60!
80!
100!
0! 1! 2! 3! 4! 5!
Foam
-act
ive
prot
ein
(mg/
L)!
HA
prot
ein
or p
olyp
heno
l!
Bentonite added (g/L)! Siebert & Lynn. J. Amer. Soc. Brew. Chem. 55: 73-78, 1997.!
The Effects of Silica Gel on Haze-Active (HA) and Foam Active (FA) Beer Constituents!
FA protein (B)!HA protein (F)!HA polyphe (J)!
B! B!B! B!
B!
J!J!J!
J! J!
F!
F!F!
F! F!
0!
40!
80!
120!
160!
0!
20!
40!
60!
80!
100!
0! 1! 2! 3! 4! 5!
Foam
-act
ive
prot
ein
(mg/
L)!
HA
prot
ein
or p
olyp
heno
l!
Silica gel added (g/L)! Siebert & Lynn. J. Amer. Soc. Brew. Chem. 55: 73-78, 1997.!
Possible Protein Adsorbent Action!
Polyphenol molecule!
Protein molecule with fixed!number of polyphenol
binding sites!
Protein molecule with no polyphenol binding sites (i.e. foam protein)!
A!d!s!A!d!s!
A!d!s!A!d!s!
Effect of Silica Gel on the Haze Forming Activities and Concentrations of Polyproline and Polyglutamine!
B!
B!B! B!
B!
J! J! J! J! J!
0!5!
10!15!20!25!30!35!
0! 1! 2! 3! 4! 5!
BCA
Prot
ein
(mg/
L)!
Silica gel treatment (g/L)!
B
B B
B
B
J J J J 0!
40!
80!
120!
160!
0! 1! 2! 3! 4! 5!
Cat
echi
n In
duce
d H
aze
(NTU
)!
Silica gel treatment (g/L)!
B Polypro!J Polygln!
J
Siebert & Lynn. J. Amer. Soc. Brew. Chem. 55: 73-78, 1997.!
Bentonite indiscriminately removes protein including both haze-active and foam-active protein.!
Silica specifically removes most of the beer haze-active protein with little effect on foam-active protein at typical treatment levels.!
Silica selectivity is due to chemical interaction, and not pore or particle sizes other than their effects on accessible surface area.!
B B B B B
J
J
J
J J
F
F
F F
F
0!
40!
80!
120!
160!
0!
20!
40!
60!
80!
100!
0! 1! 2! 3! 4! 5!
FA p
rote
in (m
g/L)!
HA
prot
ein
or p
olyp
heno
l!PVPP added (g/L)!
The Effect of PVPP on Haze-Active (HA) and Foam-Active (FA) Beer Constituents!
FA protein (B)!HA protein (F)!HA polyphe (J)!
Siebert & Lynn. J. Amer. Soc. Brew. Chem. 55: 73-78, 1997.!
Possible Polyphenol Adsorbent Action!
Polyphenol molecule!
Protein molecule with fixed!number of polyphenol
binding sites!
Protein molecule with no polyphenol binding sites (i.e. foam protein)!
A!d!s! A!d!s!
A!d!s!
Haze particle size affects!Sedimentation!
Cold storage!Centrifugation!
Filtration!Mash separation!Final filtration!
Polyphenol ≈ Protein!
Polyphenol >> Protein!Polyphenol << Protein!
Larger Particle Size!
Smaller Particle Size!
A more detailed study was carried out in pH 4.5 buffer model systems (0% ethanol) in which each of 5 concentrations of gliadin were combined with each of 6 levels of tannic acid. The haze intensities were measured and particle size distribution patterns were estimated with a laser diffraction particle size analyzer. !!Siebert & Lynn, Effect of protein/polyphenol ratio on the size of haze particles, J. Amer. Soc. Brew. Chem. 58: 117-123, 2000.!
50!50!
50!
100!
100!
100!
150!
150!
100!
200!
300!
400!
500!
40! 60! 80! 100! 120! 140!G
liadi
n (m
g/L)!
Tannic acid (mg/L)!
Haze (NTU)! 5:1!
2:1!
Haze intensity at various gliadin and TA levels at pH 4.5!
Siebert & Lynn, J. Amer. Soc. Brew. Chem. 58: 117-123, 2000.!
180!180!
60!
20!
60!
100! 100!
140! 140!
20!
Haz
e (N
TU)!
500!400!
300!200!Gliadin
(mg/L)!
140!120!100!
80!60!40! Tannic acid (mg/L)!
Summary!Protein-polyphenol haze intensity is strongly
affected by protein/polyphenol ratio and pH.!Haze particle size is also affected by protein/
polyphenol ratio, with the largest size particles at intermediate ratios. Changes are more in the proportions of particles of discrete sizes than of gradual shifts of a monomodal population.!
Particle size should impact sedimentation and filtration performance. !
Summary (continued)!Beer HA protein and HA polyphenol interact
strongly (causing the greatest haze intensity with fixed amounts of protein and polyphenol) near pH 4.2. The amount of haze formed declines sharply at both higher and lower pH.!
This appears to affect haze removal during beer maturation, fining activity and the effectiveness of silica and PVPP.!
Summary (continued)!The beer proteins that are involved in haze
formation are rich in proline and derived from barley hordein.!
The beer polyphenols involved in haze bridge proteins together to form complexes. These are primarily ‘dimeric’ proanthocyanidins. They originate mainly from malt but hops can also contribute.!
Summary (continued)!The time course of haze formation has an
initial lag followed by an essentially linear increase. The latter is a function of the product of HA protein and HA polyphenol concentrations.!
As a result, reducing either HA protein or HA polyphenol by a similar percentage should have a similar stabilizing effect.!
Summary (continued)!Silica attaches to the same sites in HA proteins
to which polyphenols attach. This makes it specific for HA protein and largely spares the foam-active protein.!
PVPP resembles the proline sites in HA proteins and competes for the HA polyphenol. It is at most able to remove about half of the beer HA polyphenol because most of that is already attached to beer HA proteins. !
Acknowledgments!This research was carried out with major support
f rom Suntory Ltd. , Osaka, Japan and International Specialty Products, Wayne, NJ.!
Unchillproofed beer was donated by Genesee Brewing Company, Rochester, NY.!
Karl J. Siebert!Professor of Biochemistry!
Dept. of Food Science!Cornell University!
Geneva, NY [email protected]!
http://web.foodscience.cornell.edu/siebert!
Review Articles on Haze!K.J. Siebert, Haze in beverages. In Advances in Food and Nutrition Research 57: 53-86, 2009.!K.J. Siebert, Haze formation in beverages. LWT - Food Science and Technology, 39: 987-994, 2006.!K.J. Siebert, Effects of protein-polyphenol interactions on beverage haze, stabilization, and analysis. Journal of Agricultural and Food Chemistry, 47: 353-362, 1999.!