Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College -...
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Transcript of Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College -...
![Page 1: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/1.jpg)
Basic science as it applies to forensic analysis
Rebecca Hailey, Florida State College - Jacksonville Chemistry
Yousef Hijji, Morgan State U ChemistryRachel Mohr, WVU Forensic Science
Martin Overly, WVU Forensic ScienceElizabeth Pollock, Richard Stockton College of NJ,
ChemistryJacqueline Speir, WVU Forensic Science
Shuo Wei, WVU Biology
![Page 2: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/2.jpg)
Target audience
• Introductory biology• Second semester general chemistry• Second semester general, organic, biological
chemistry• Sophomore level forensic science students
![Page 3: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/3.jpg)
Learning goals and outcomes
Learning Goals• Students will learn about
enzyme binding and specificity
• Will understand the role of inhibitors
• Apply knowledge to novel situation
Learning Outcomes• Will be able to predict reactions
between given enzymes and substrates
• Will be able to interpret enzyme assay test results
• Will be able to predict the consequences of the addition of an inhibitor
• Will be able to relate the theoretical understanding of enzymes to real life applications
![Page 4: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/4.jpg)
What do we need to know to solve this case?
What do we know about saliva?
How is it different from other body fluids?
![Page 5: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/5.jpg)
Which of the following components of saliva should we target for detection?
A. WaterB. Salts C. CarbohydratesD. Food particlesE. Salivary enzymes = amylase
![Page 6: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/6.jpg)
Demonstration of Phadebas test for the presence of amylase
![Page 7: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/7.jpg)
Which of the following is a positive test for saliva?
![Page 8: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/8.jpg)
How enzymes workIdentify the components a-d
enzyme substrateactive site product
![Page 9: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/9.jpg)
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrateactive site product
![Page 10: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/10.jpg)
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
Substrate (e.g. starch)
active site product
![Page 11: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/11.jpg)
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrate (e.g. starch)
active site
product
![Page 12: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/12.jpg)
How enzymes workIdentify the components a-d
enzyme (e.g. amylase)
substrate (e.g. starch)
active site
product
animation
![Page 13: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/13.jpg)
Which one(s) of the following contains molecules which can serve as
substrates for amylase?
A. ButterB. Dietary fiber/celluloseC. Jolly RanchersD. Egg whitesE. Potato chips
![Page 14: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/14.jpg)
Has the suspect been cleared, or looking increasingly guilty?
![Page 15: Basic science as it applies to forensic analysis Rebecca Hailey, Florida State College - Jacksonville Chemistry Yousef Hijji, Morgan State U Chemistry.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649e585503460f94b51a98/html5/thumbnails/15.jpg)
Pharmaceutical case study
You are a scientist at a pharmaceutical company and you are tasked with coming up with a pill that will mimic the Atkins diet (but, still allowing dieters to consume carbohydrates). What do you need to know to do your job?