ASSOCIATED UNIT EEZ(CSIC)-UJI ASSOCIATED UNIT EEZ(CSIC)-UJI RESEARHRESEARH

http://nitropriming.wordpress.com/http://nitropriming.wordpress.com/

Metabolic integration and Cell Signaling Group. Metabolic integration and Cell Signaling Group. Departamento de Ciencias Agrarias Departamento de Ciencias Agrarias

y del Medio Natural (CAMN). y del Medio Natural (CAMN). Universitat Jaume IUniversitat Jaume I

Research Lines:Research Lines:

Induced resistance against pathogens

Development of resistance inducers and priming agents. Chromatography

applied to plant defense.

Nitrogen transport

Role of systemin in the establishment of mycorrhizas and induction of systemic

resistance in tomato

Study of Plant-arthropod interactions

Reactive oxygen species and hormonal metabolism in defense priming

Balances metabólicos y Balances metabólicos y hormonales implicados en hormonales implicados en

la resistencia inducida la resistencia inducida frente a patógenosfrente a patógenos

ACCUMULATION OF TF->FASTER

GENE TRANSCRIPTION

OR PR ACCUMULATION

EPIGENETIC CHANGESHISTONE

ACETYLATION or DNA

METHYLATION

PHOSPHORILATION CONTROL

MAPK CASCADES

2ND BIOTIC STRESS STRONG DEFENCESTRONG DEFENCE

STRESS MEMORY

BASAL DEFENCEBASAL DEFENCE(WEAK AND SLOW)(WEAK AND SLOW)

DIS

EA

SE

D P

LAN

T

DIS

EA

SE

D P

LAN

T

HE

ALT

HY

PLA

NT

HE

ALT

HY

PLA

NT

STRESSPRE-EXPOSUREPRIMING AGENT

METABOLITE METABOLITE CONJUGATIONCONJUGATION

HORMONAL HORMONAL REGULATION REGULATION

ROSROShomeostasishomeostasis

5

Search of hormone relatives using PRECURSOR ION Search of hormone relatives using PRECURSOR ION SCANNING and targeted MRMSCANNING and targeted MRM

PIS

-P

IS- T

arg

eted

Met

abo

lom

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eta

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sDesarrollo de técnicas cromatográficas Desarrollo de técnicas cromatográficas para el estudio de la resistencia inducidapara el estudio de la resistencia inducida

3 different priming stimuli

Common Common metabolomic metabolomic fingerprint of fingerprint of

primingpriming

Non targeted Metabolomic Approaches Non targeted Metabolomic Approaches

PROPUESTA DE COLABORACIÓNPROPUESTA DE COLABORACIÓN

INVESTIGACIÓN A CORTO PLAZO EN FUNCIÓN DEL CONOCIMIENTO DE PARTIDA

POOL OF METABOLITE WITHPRIMING OR INDUCEDRESISTANCE POTENTIAL

Stimuli: resistant genotypes from the germplasm bank; treatments; other related plant species with enhanced resistance

Stimulus 1

Stimulus 2

Stimulus 3

Current Group Members Dr Víctor Flors; TU Dr Miguel Cerezo; TU Dra Paloma Sánchez Bel, RyC Jordi Gamir, PhD student Blas Agut, PhD student

http://nitropriming.wordpress.com/http://nitropriming.wordpress.com/

New coming members:

Dra. Victoria Pastor

Plants can increase their level of resistance is by intensifying the responsiveness of their immune system upon recognition of selected signals from their environment. This so-called priming of defence can provide a memory of resistance, which is based on a faster and/or stronger defence reaction upon pathogen or pest attack.

Priming can target various layers of induced defence. Priming ranges from early responses controlled by adjustments in hormone-dependent signalling pathways and ROS to long lasting events

Pastor, V., Luna, E., Mauch-Mani, B., Ton, J., Flors, V. Primed plants do not forget (2012) Environmental and Experimental Botany.

SA conjugates

Indolic metabolites