BalanCD HEK293 System - Irvine Scientific€¦ · 72h 72h 48h 24h Figure 1. HEK293T cells were...
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BalanCD HEK293 System BalanCD HEK293 is a scalable system designed to support growth and transfection of HEK293 cell lines in suspension cultures. Comprised of BalanCD HEK293 medium, BalanCD HEK293 Feed and Anti-Clumping Supplement, this highly versatile system supports a range of applications including production of viral vectors for gene therapy, transient protein expression, and recombinant protein production. BalanCD HEK293 is part of the scalable BalanCD media and supplements platform designed to provide the optimal balance between growth and production to maximize productivity from mammalian cell cultures. Use the same growth medium at every step and every scale Maximize productivity in suspension cultures Versatile formulation supports transfection and production including: • Rapid, scalable production of viral vectors • Use the same system for a wide range of HEK293 cell lines • Increased productivity in transient protein expression Fulfills regulatory requirements: • Chemically-defined, animal component-free • Drug Master File (DMF) filed with US FDA Scalable protocol for use in flasks, spinner flasks, and bioreactors Maintain cell stocks Seeding Transfection Production Transient expression and protein production Viral vector production BalanCD HEK293 (Anti-Clumping Supplement optional) BalanCD HEK293 (Anti-Clumping Supplement optional) BalanCD HEK293 BalanCD HEK293 Feed (Anti-Clumping Supplement optional) BalanCD HEK293 BalanCD HEK293
Transcript of BalanCD HEK293 System - Irvine Scientific€¦ · 72h 72h 48h 24h Figure 1. HEK293T cells were...
BalanCD HEK293 System
BalanCD HEK293 is a scalable system designed to support growth and transfection of HEK293 cell lines in suspension cultures. Comprised of BalanCD HEK293 medium, BalanCD HEK293 Feed and Anti-Clumping Supplement, this highly versatile system supports a range of applications including production of viral vectors for gene therapy, transient protein expression, and recombinant protein production. BalanCD HEK293 is part of the scalable BalanCD media and supplements platform designed to provide the optimal balance between growth and production to maximize productivity from mammalian cell cultures.
Use the same growth medium at every step and every scale
Maximize productivity in suspension cultures Versatile formulation supports transfection and production including:
• Rapid, scalable production of viral vectors
• Use the same system for a wide range of HEK293 cell lines
• Increased productivity in transient protein expression
Fulfills regulatory requirements:
• Chemically-defined, animal component-free
• Drug Master File (DMF) filed with US FDA
Scalable protocol for use in flasks, spinner flasks, and bioreactors
Maintain cell stocks
Seeding Transfection Production
Transient expression and protein production
Viral vector production
BalanCD HEK293 (Anti-Clumping Supplement optional)
BalanCD HEK293 (Anti-Clumping
Supplement optional)
BalanCD HEK293 BalanCD HEK293 Feed
(Anti-Clumping Supplement optional)
BalanCD HEK293
BalanCD HEK293
With you at every step From development through to large-scale production, success is highly dependent on providing the optimal culture conditions. The BalanCD platform of cell-specific growth media and feeds has been developed to avoid many of the challenges faced during cell line development through process optimization and into commercial production. These serum-free, chemically-defined media and their supplements provide the optimal balance between growth and production to maximize productivity at any scale.
Media optimization and customizationBeginning with the most suitable BalanCD medium reduces development and optimization times necessary to achieve the required yield and quality of end-product. For those requiring customization to meet a specific need, experienced FUJIFILM Irvine Scientific professionals can provide efficient, cost-effective and time-saving assistance.
Rapid prototypingNormally provided within 10 working days, a rapid prototyping service offers flexible, small-scale, non-GMP media production of liquid and powder formulations. By using the same raw materials sourced and quality-controlled as for our large-scale GMP manufacturing, this approach greatly facilitates the step from
research to process development activities.
A smooth transition into commercial productionFUJIFILM Irvine Scientific products and services are developed according to the highest medical standards. Every BalanCD product is subject to a stringent Quality System unrivalled in the industry. To fulfill quality and reliability requirements, proprietary and customized media are manufactured under fully cGMP-compliant conditions in dedicated animal component- free, state-of-the-art facilities replicated in California and Japan. Comprehensive documentation, including information from Supply Chain Management through to Drug Master Files, is available to help minimize the regulatory burden.
• FDA-regulated
• cGMP compliant manufacture
• ISO13485,
EN 13485:2016 certified
• Drug Master Files
To discuss your requirements, contact us at [email protected] or visit our website at www.irvinesci.com/contact-us
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Figure 1. HEK293T cells were cultured in BalanCD HEK293 medium in a 2 L working volume bioreactor and passaged every 3 –4 days. PEI-mediated transfection of 4 plasmids was performed 3 days after seeding at a DNA:PEI ratio of 1:1.5. Viable cell density was determined. Lentivirus titer was measured at 24, 48, and 72 hours post transfection.
Figure 2. HEK293VC cells were cultured in a 2 L working volume bioreactor. PEI-mediated transfection of 3 plasmids was performed 3 days after seeding at a DNA:PEI ratio of 1:1.5. Cell diameter were determined. AAV titer was measured at 48, 72, and 96 hours post-transfection.
Rapid, scalable production of viral vectors
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Improved productivity of Adeno-Associated Virus (AAV) to a commercially-available medium
Simple, scalable protocol supports cell growth and increases yield of lentiviral vector
• Achieve optimal production yields within 48 hours
• Use the same medium for transfection and production
• Add Anti-Clumping Supplement to prevent aggregation
Bioreactor data courtesy of Généthon, Evry Cedex, France.
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Figure 3. HEK293-6E cells were cultured in duplicate 125 mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection. Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures were terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.
Increase productivity in transient protein expression
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MallardPMS 5405
CMYK 68-35-17-49
RGB 68-105-125
HEX #44697d
Sea GreenPMS 7486
CMYK 28-0-54-0
RGB 185-224-156
HEX #b9e09c
SandPMS 5807
CMYK 19-11-36-0
RGB 208-209-175
HEX #d0d1af
TealPMS 7472
CMYK 63-7-33-0
RGB 83-184-179
HEX #53b8b3
MauvePMS 7654
CMYK 34-58-22-1
RGB 175-122-151
HEX #af7a97
Cool GrayPMS 7543
CMYK 43-29-25-0
RGB 151-164-174
HEX #97a4ae
SkyPMS 2905
CMYK 45-14-3-0
RGB 134-188-219
HEX #86bcdb
CharcoalPMS Cool Gray 10
CMYK 62-53-47-19
RGB 99-101-105
HEX #636569
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ColorThe foundational colors comprise the blue from
FUJIFILM Irvine Scienti�c and Fuji�lm Advanced Green.
These colors are very bold and should be used with
care so as to maintain elegance and balance in the
designs. A range of eight additional colors is provided
to support design options and complement the primary
brand colors.
FISI BluePMS 300
CMYK 100-44-0-0
RGB 0-122-194
HEX #007ac2
Fujifilm Advanced GreenPMS 340
CMYK 90-0-70-0
RGB 1-145-109
HEX #00916d
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Two-fold increase in titer compared to other commercially-available media
• Achieve gram-scale yields of proteins using a simple scalable protocol
• Grow, transfect, and produce in BalanCD HEK293 medium
• Maximize yield by adding BalanCD HEK293 Feed and Anti-Clumping Supplement post-transfection
Titer post-transfection
Figure 4. Three different commercially available HEK293 cell lines were cultured in 125 mL shaker flasks, at a working volume of 20–25%. Cells were seeded at 3 x105 cells/mL, in duplicate, BalanCD HEK293 medium, supplemented with Anti-Clumping Supplement, and fed on days 3, 4, 5, and 6, at 5% v/v. Data shown represents the average of two duplicate cultures.
Use the same system for a wide range of HEK293 cell lines
Comparison with other commercially-available media
• Achieve higher cell densities while maintaining viability
• Increase culture longevity and maximize growth by adding BalanCD HEK 293 Feed
• Add Anti-Clumping Supplement to prevent aggregation
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Supplier A-2
Supplier A-3
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MallardPMS 5405
CMYK 68-35-17-49
RGB 68-105-125
HEX #44697d
Sea GreenPMS 7486
CMYK 28-0-54-0
RGB 185-224-156
HEX #b9e09c
SandPMS 5807
CMYK 19-11-36-0
RGB 208-209-175
HEX #d0d1af
TealPMS 7472
CMYK 63-7-33-0
RGB 83-184-179
HEX #53b8b3
MauvePMS 7654
CMYK 34-58-22-1
RGB 175-122-151
HEX #af7a97
Cool GrayPMS 7543
CMYK 43-29-25-0
RGB 151-164-174
HEX #97a4ae
SkyPMS 2905
CMYK 45-14-3-0
RGB 134-188-219
HEX #86bcdb
CharcoalPMS Cool Gray 10
CMYK 62-53-47-19
RGB 99-101-105
HEX #636569
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ColorThe foundational colors comprise the blue from
FUJIFILM Irvine Scienti�c and Fuji�lm Advanced Green.
These colors are very bold and should be used with
care so as to maintain elegance and balance in the
designs. A range of eight additional colors is provided
to support design options and complement the primary
brand colors.
FISI BluePMS 300
CMYK 100-44-0-0
RGB 0-122-194
HEX #007ac2
Fujifilm Advanced GreenPMS 340
CMYK 90-0-70-0
RGB 1-145-109
HEX #00916d
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Supplier A-1
Supplier A-2
Supplier A-3
Supplier B
MallardPMS 5405
CMYK 68-35-17-49
RGB 68-105-125
HEX #44697d
Sea GreenPMS 7486
CMYK 28-0-54-0
RGB 185-224-156
HEX #b9e09c
SandPMS 5807
CMYK 19-11-36-0
RGB 208-209-175
HEX #d0d1af
TealPMS 7472
CMYK 63-7-33-0
RGB 83-184-179
HEX #53b8b3
MauvePMS 7654
CMYK 34-58-22-1
RGB 175-122-151
HEX #af7a97
Cool GrayPMS 7543
CMYK 43-29-25-0
RGB 151-164-174
HEX #97a4ae
SkyPMS 2905
CMYK 45-14-3-0
RGB 134-188-219
HEX #86bcdb
CharcoalPMS Cool Gray 10
CMYK 62-53-47-19
RGB 99-101-105
HEX #636569
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ColorThe foundational colors comprise the blue from
FUJIFILM Irvine Scienti�c and Fuji�lm Advanced Green.
These colors are very bold and should be used with
care so as to maintain elegance and balance in the
designs. A range of eight additional colors is provided
to support design options and complement the primary
brand colors.
FISI BluePMS 300
CMYK 100-44-0-0
RGB 0-122-194
HEX #007ac2
Fujifilm Advanced GreenPMS 340
CMYK 90-0-70-0
RGB 1-145-109
HEX #00916d
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Days in culture
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BalanCD HEK293 System
Supplier A-1
Supplier A-2
Supplier A-3
Supplier B
MallardPMS 5405
CMYK 68-35-17-49
RGB 68-105-125
HEX #44697d
Sea GreenPMS 7486
CMYK 28-0-54-0
RGB 185-224-156
HEX #b9e09c
SandPMS 5807
CMYK 19-11-36-0
RGB 208-209-175
HEX #d0d1af
TealPMS 7472
CMYK 63-7-33-0
RGB 83-184-179
HEX #53b8b3
MauvePMS 7654
CMYK 34-58-22-1
RGB 175-122-151
HEX #af7a97
Cool GrayPMS 7543
CMYK 43-29-25-0
RGB 151-164-174
HEX #97a4ae
SkyPMS 2905
CMYK 45-14-3-0
RGB 134-188-219
HEX #86bcdb
CharcoalPMS Cool Gray 10
CMYK 62-53-47-19
RGB 99-101-105
HEX #636569
13
ColorThe foundational colors comprise the blue from
FUJIFILM Irvine Scienti�c and Fuji�lm Advanced Green.
These colors are very bold and should be used with
care so as to maintain elegance and balance in the
designs. A range of eight additional colors is provided
to support design options and complement the primary
brand colors.
FISI BluePMS 300
CMYK 100-44-0-0
RGB 0-122-194
HEX #007ac2
Fujifilm Advanced GreenPMS 340
CMYK 90-0-70-0
RGB 1-145-109
HEX #00916d
12
HEK293.2SUS
EXPI293-F
Days in culture
FreeStyle and Expi293-F are trademarks of ThermoFisher Scientific.; HEK292.2sus from ATCC
FREESTYLE 293-F
FUJIFILM Irvine Scientific and its logo, and BalanCD are registered trademarks of FUJIFILM Irvine Scientific, Inc. in various jurisdictions. All other trademarks are the property of their respective owners. ©2020 FUJIFILM Irvine Scientific. P/N 003441Rev.01
www.irvinesci.com
Ordering InformationItem Catalog # Size*
BalanCD HEK293, liquid 91165-1 L 1 L
BalanCD HEK293, powder 94137-10 L 10 L
BalanCD HEK293 Feed, liquid 91166-500 mL 500 mL
Anti-Clumping Supplement, liquid 91150-50 mL 50 mL Animal component-free formula
*Custom sizes and packaging available on request.
Additional Information
With over 45 years of industry experience, FUJIFILM Irvine Scientific is focused on providing the highest quality cell culture media. We are dedicated to bringing life to products, and ultimately, to the patients who benefit from the resulting therapeutic advances. World-renowned for our unwavering commitment to full-spectrum partnership, FUJIFILM Irvine Scientific has defined the industry standard in support, innovation, and best-in-class turnaround time.
BalanCD HEK293, Anti-Clumping Supplement, BalanCD HEK293 Feed
Chemically-defined, animal component-free formula
