Balamurugan N. Appakalai, Ph.D. Clinical Islet Cell ... · Balamurugan N. Appakalai, Ph.D. Clinical...

Balamurugan N. Appakalai, Ph.D.

Clinical Islet Cell Laboratory Cardiovascular Innovation Institute

302 East Muhammad Ali Blvd Louisville, KY, 40202

(502) 852-1131 (502) 854-0558

[email protected]

EDUCATION 1990-1993 M.S., Medical Physiology, Madras University, Chennai, TN, India 1993-1998 Ph.D., Islet Cell Transplantation, Christian Medical College, Vellore, TN, India 1999-2000 JSPS Fellowship, Bio-artificial pancreas transplantation, Kyoto University, Kyoto, Japan ACADEMIC APPOINTMENTS 2001-2002 Research Associate Department of Surgery, Thomas E. Starzl Transplantation Institute University of Pittsburgh Pittsburgh, PA 2003-2004 Instructor, Co-Director -Islet Manufacturing Core Department of Surgery, Thomas E. Starzl Transplantation Institute University of Pittsburgh Pittsburgh, PA 2005-2006 Assistant Professor, Co-Director -Islet Manufacturing Core Department of Surgery, Thomas E. Starzl Transplantation Institute University of Pittsburgh Pittsburgh, PA 2006-2007 Assistant Professor, Director -Clinical Islet cGMP Facility Barbara Davis Center for Childhood Diabetes School of Medicine, University of Colorado at Denver Denver, CO 2007-2014 Assistant Professor, Director -Islet Processing Core Department of Surgery, Schulze Diabetes Institute University of Minnesota

Minneapolis, MN 2013-2014 Associate Director -Schulze Diabetes Institute Department of Surgery University of Minnesota

Minneapolis, MN 2014-2014 Associate Professor, Director -Islet Processing Core Department of Surgery, Schulze Diabetes Institute

mailto:[email protected]

Balamurugan N. Appakalai, PhD, September 2016 of 31

University of Minnesota Minneapolis, MN

2014-Present Associate Professor, Director-Clinical Islet Cell Laboratory Department of Surgery, Cardiovascular Innovation Institute

University of Louisville Louisville, KY

OTHER POSITIONS AND EMPLOYMENT N/A CERTIFICATION AND LICENSURE N/A PROFESSIONAL MEMBERSHIPS AND ACTIVITIES 2002- Present Ad hoc Reviewer, Pancreas 2003- Present Ad hoc Reviewer, American Journal of Transplantation 2003- Present Ad hoc Reviewer, Life Sciences 2004- Present Ad hoc Reviewer, Cell Transplantation 2005- Present Ad hoc Reviewer, Xenotransplantation 2008- Present Ad hoc Reviewer, Current Diabetes Reviews 2008- Present Ad hoc Reviewer, Diabetes 2008- Present Ad hoc Reviewer, Diabetologia 2010- Present Ad hoc Reviewer, Hepatobiliary & Pancreatic Diseases International 2012- Present Ad hoc Reviewer, Biotechnology and Applied Biochemistry

HONORS AND AWARDS 1995 R. Srinivasan Prize (Best Research Paper Award)

("Effect of Cyclosporine-A on Xenotransplantation of Monkey Pancreatic Islets into Rats") Association of Physiologists and Pharmacologists of India’s 41st National Conference, December 1995, Belgaum, Karnataka, India.

1996 R. Srinivasan Prize (Best Research Paper Award) ("Insulin Secretory Characteristics of Cultured Monkey Pancreatic Islets")


Association of Physiologists and Pharmacologists of India’s 42nd National Conference, December 1996, Cuttack, Orissa, India.

1997 Dr. N. Padmanabam Memorial Award (Best Research Paper Award)

("Isolation, Insulin Secretion, Culture and Transplantation of Monkey Pancreatic Islets") Indian Association of Biomedical Scientists 18th National Conference, October 1997, Delhi, India.

1999 JSPS Fellowship (“Studies on Islet Biology and Bio-artificial Pancreas Transplantation”) Japan Society for the Promotion of Sciences, Govt. of Japan, Japan 2002 Shelly Patrick Fellowship (“Studies on Human Islet Cell Functional Mass Preservation”)

Jonathan S. Patrick Foundation, Thomas E. Starzl Transplantation, June 2002, Pittsburgh, USA. 2005 Young Investigator Award (Best Research Paper Award)

(“Harmful Delayed Effects of Exogenous Isolation Enzymes on Isolated Human Islets: Relevance to Clinical Transplantation”). 10th World Congress of the International Pancreas and Islet Transplantation Association, May 2005, Geneva, Switzerland.

2013 A. V. Tilak Parvathi Devi Prize (Best paper in Endocrinology) (“Antiapoptotic actions of exendin-4 against hypoxia and cytokines are augmented by CREB”)

Association of Physiologists and Pharmacologists of India – 59th National Conference, November 2013, Bangalore, India.

COMMITTEE ASSIGNMENTS AND ADMINISTRATIVE SERVICES 2006-2008 Member, Cellular Transplantation Committee, American Society of Transplantation (AST). 2007-2013 Chair, NIH sponsored Clinical Islet Transplantation (CIT) Consortium Islet Manufacturing Site

Qualification Committee 2009-Present Member, NIH sponsored Clinical Islet Transplantation (CIT) Consortium Islet Manufacturing publication

Committee 2009-2014 Member, National Institute for Diabetes and Digestive and Kidney Diseases- and Juvenile Diabetes

Research Foundation- sponsored Collaborative Islet Transplant Registry (CITR) Publication Committee 2016- Member, American Diabetes Association's Grant Review Committee INVITED TALKS 2016 Vanderbilt Diabetes Research and Training Center, Vanderbilt University, Nashville, TN; September 29, 2016. Talk: “Strategies to Improve Human Pancreatic Islet Yield and Function”


GEM Hospital (Gastroenterology Medical Centre and Hospital), Coimbatore, TN, India; August 2, 2016. Talk: “Islet cell autotransplantation for the treatment of chronic pancreatitis”.

Chettinad Hospital and Research Institute (Chettinad Academy of Research and Education), Chennai, TN, India; 25th July 2016. Talk: “New Methods to Improve Human Pancreatic Islet Cell Mass and Quality”.

Chettinad Hospital and Research Institute (Chettinad Academy of Research and Education), Chennai, TN, India; 26th July 2016. Talk: Human Pancreatic Islet Cells for the Treatment of Diabetes: Updates of clinical transplantation and islet biology research”.

2016 American Transplant Congress: Luncheon Workshop: Total Pancreatectomy and Islet Autotransplantation, May 11-15, 2016 (Member of the organizing committee). Talk: “How to isolate high islet mass from chronic pancreatitis pancreas with good quality”.

LifeNet Health (Organ Procurement Organization), Virginia Beach, VA, May 6, 2016. Talk: “Recent Advances in Human Islet Isolation Technique and Clinical Transplantation Outcome”.

Department of Physiology, University of Louisville, March 29, 2016. Talk: “Current status of Pancreatic Islet Cell Transplantation for the treatment of Diabetes”. 2015 “Which donor and isolation factors predict successful islet isolations and successful islet transplant outcomes?” 2015 Joint Congress of the International pancreas and islet transplantation association (IPITA-IXA-CTS held in Melbourne, Australia, 15–19 November 2015. Pre-symposium on Islet Isolation and Transplantation: where are we now and where are we going? “Islet cell transplantation therapy for diabetes treatment” INDO-GLOBAL HEALTHCARE SUMMIT & EXPO 2015, Hyderabad, India July 23 to 26, 2015. “Current status of islet Cell auto - allo - and xeno - transplantation for thetreatment of Diabetes” ISLETCON 2015- Diabetes Mellitus, a recent update: organized by the Sree Balaji Medical College and Hospital, Chennai, India, July 27-29, 2015. “How to maximize islet yield from chronic pancreatitis pancreas”2015 American Transplant Congress: Luncheon Workshop: Total Pancreatectomy and Islet Autotransplantation, May 2015 (Member of the organizing committee). “Recent trend in Islet cell auto-, allo-, xeno-transplantation” Surgery Department Grand Rounds in King Faisal Hospital and Research Center, Riyadh, Saudi Arabia, February 2015. “Research in islet cell transplant: What is next? Continuing Medical Education seminar series: King Faisal Hospital and Research Center, Riyadh, Saudi Arabia, February 2015. International Transplantation Society organized Inaugural Webinar Series for International Pancreas and Islet Transplantation Association: "State of the art in islet isolation-Do’s and Don’t", January 2015. 2014


“Recent progress in pancreatic islet cell transplantation” Division of Endocrinology, Metabolism and Diabetes Multidisciplinary Endocrine Conference Grand Rounds, University of Louisville; November 2014 “Updates on islet cell transplantation for the treatment of diabetes” 14th Congress of the Middle East Society for Organ Transplantation, Istanbul, Turkey, September 2014. “Optimizing Islet Isolation from the Diseased Pancreas” NIH-NIDDK organized-Total Pancreatectomy with Islet Auto-Transplantation (TP-IAT): Gaps, Needs, and Opportunities” Workshop, Pittsburgh, Pennsylvania, July 2014. “Maximizing Porcine Islet Yield for Xenotransplantation” 9th Seoul Forum on Xenotransplantation, Seoul, March 2014. “Islet cell transplantation for the treatment of type 1 diabetes” Seoul National University, Graduate School Lecture, March 2014. “Newer Strategies to Improve Islet Yield and Engraftment of Autologous Islets” International Symposium on the Medical & Surgical Treatment of Chronic Pancreatitis, Kiawah Island Golf Resort, South Carolina, February 2014. 2013 “Source of pig, islet manufacturing and releasing criteria” Mature Pig session, First International Conference of Clinical Islet Xenotransplantation, Osaka, Japan, November, 2013. “Technical Aspects of Pig Islet Isolation”. Early morning workshop, The Joint Congress of International Xenotransplantation Association and Organ Transplantation in ABO-incompatible and hyperimmunized recipients, Osaka, Japan, November, 2013. “Present and future perspective of islet transplantation.” Symposium, 13th Congress of the Asian Society of Transplantation (CAST 2013), Kyoto, Japan, September 2013. “Challenges of islet isolation for islet auto transplantation” Pre-Congress Symposium on Islet Isolation: 14th World Congress of the International Pancreas and Islet Transplant Association, September 2013, Monterey, CA. “Unique aspects of islet isolation from pancreas with chronic pancreatitis: Morning Workshop 3: Autologous Islet Transplantation: 14th World Congress of the International Pancreas and Islet Transplant Association, September 2013, Monterey, CA. “Islet cell transplantation: nearing cure for diabetes.” Key note presentation, Hormones 2013 (time for clarity), meeting of Tamilnadu Endocrine Society, Madurai, Tamilnadu, India, June 2013. “Maximizing Islet Yield from Chronic Pancreatitis Glands for Islet Auto-transplantation.” State-of-the-Art Lecture, American Diabetes Association 73rd Scientific Sessions, Chicago, Illinois, June 2013. “Recent developments in islet cell transplantation.” University of Maryland, Transplant surgery seminar, May 2013. “Current progresses in islet cell auto-, allo- and xeno-transplantation.” Cleveland Clinic, Transplant surgery grand rounds, April 2013. 2012


“Islet research: Historical experience in India.” Inaugural Lecture, Indo-US Bilateral workshop on Pancreatic Islets –from isolation to transplantation, Hyderabad, India, September 2012. “Methods to improve islet yield.” Key note presentation, Indo-US Bilateral workshop on Pancreatic Islets –from isolation to transplantation, Hyderabad, India, September 2012. 2011 “Technical aspects of human islet isolation from chronic pancreatitis pancreas for islet autotransplantation.” Workshop on Total Pancreatectomy and Islet Autotransplant for Treatment of Chronic Pancreatitis, 2011 American Pancreatic Association Annual Meeting, Chicago, Illinois, November 2011. “Technical Aspects of Human Islet Isolation for Islet Auto Transplantation.” Early Morning Workshop: Islet auto-transplantation, American Transplantation Congress, Philadelphia, Pennsylvania, May 2011. “Currently available collagenases for human islet isolation-the Minneapolis experience.” Symposium on ongoing challenges of human islet isolation, 13th World Congress of The International Pancreas and Islet Transplantation Association, Prague, Czech Republic, June 2011. “University of Minnesota experience with a new enzyme mixture.” Invited talk, Collaborative Islet Transplantation Registry 2011 annual meeting, Arlington, VA, April 2011. 2010 “Recent progress in islet cell auto, allo and xeno-transplantation for the treatment of type 1 diabetes.” Symposium, Endocrine Society of India 40th annual conference at the Christian Medical College, Vellore, India, December 2010. “Clinical Transplantation of human islets for the treatment of type I diabetes and beta cell biology of human islets for type II diabetes research.” Keynote presentation, Research Society for the study of diabetes in India (RSSDI) 38th annual meeting, Kochi, Kerala, India, November 2010. “Maximizing human pancreatic islet cell yield and quality for clinical transplantation using intact C1 collagenase and alpha-1 antitryspin.” Medical University of South Carolina, Department of Surgery seminar September 2010. “Intact class 1 collagenase and neutral protease combination maximizes human islet yield from deceased & pancreatitis pancreata for transplantation and alpha-1 antitrypsin supplementation improves islet culture recovery by blocking insulin degradation effect of acinar cell proteases.”The DERC-research in progress series, Barbara Davis Center for Childhood Diabetes, University of Colorado, Denver, February 2010. “Successful recovery of human islets from pancreatitis pancreas for islet cell autologous transplantation.” Georgetown University Hospital, Department of Surgery seminar, January 2010. 2009 “High human islet yield with combined use of Clostridium histolyticum neutral protease and high proportion of intact class I collagenase.” Workshop: Collagenase Enzyme Blends for Human Islet Isolation: Efficacy, Useful Additives, Safety and cGMP/GCP-Regulations, Joint meeting of the International Pancreas and Islet Transplantation Association and International Xenotransplantation Association 2009 Meeting, Venice, Italy, October 2009. 2008


“Novel Methods for Islet Isolation.” Early morning workshop: Islet Isolation - Solving Problems, 22nd International Congress of The Transplantation Society, Sydney, Australia, August 2008. 2006 “Human Islets Isolation and Secondary Negative Effects of Isolation Enzymes”. Diabetes Institute for Immunology and Transplantation seminar series, University of Minnesota, July 2006. “Secondary Negative Effects of Isolation Enzyme(s) on Human Islets”. Second Islet Cell Resource Center (ICR) organized Islet Workshop, Minneapolis, Minnesota, August 2006. 2004 “Preservation of Isolated Human Islets Functional Mass.” Key note presentation, Joint Meeting of the 11th International Association of Pancreatology and the 35th Annual Meeting of the Japan Pancreas Society, Sendai, Japan, July 2004. EDUCATIONAL ACTIVITIES

Teaching activities 1993-1993 Lecturer, Department of Physiology, Sri Siddhartha Medical College, Tumkur, Karnataka, India 1996-1999 Lecturer of Physiology, Department of Physiology, Christian Medical College, Vellore, TN, India.

Involved in the teaching programs of the department of Physiology - for Medical students, graduate and Allied Health Science students.

Doctoral Students Advised

2009-2012 Served as one of the research advisors for doctoral thesis committee to Dr. Thomas M. Suszynski (Ph.D. in Biomedical engineering, PhD guide-Dr. Klearchos K. Papas) during the period 2009-2012. Title of the thesis: Tissue-Engineered Islet Graft Design and Assessment.

2007-2012 Served as one of the research advisors for doctoral thesis committee to Mr. William E Scott III (Ph.D. in Biomedical engineering, PhD guide-Dr. Klearchos K. Papas) during the period 2007-2012. Title of the thesis: Application of NMR in the Characterization of Existing and Development of New Methods for Pancreas Preservation Doctoral Committees Served on

2013 Ph.D. thesis examiner- The University of Adelaide, Adelaide, Australia. Reviewed Miss. Amy Hughes Ph.D. thesis on “Optimal Delivery of Therapeutic Genes to Pancreatic Islets” November 2013.

2015 The University of Adelaide, Australia: Thesis of Mariea Dencey Bosco. Topic:, “Investigations into the

Role of Zinc and Zinc Transporters in the Pathogenesis of Type 2 Diabetes in db/db Mice” May 2015 2015 Dr. NTR University of Health Sciences, Hyderabad, India: Thesis of Mr. Pavan Kumar Pondugala

“Characterization of the islet cells and pancreatic progenitors in patients with Chronic Pancreatitis’’ April 2015


Post-doctoral fellows supervised 2006-2008 Yukihide Yonekawa, M.D., Ph.D., Post-doctoral Research Associate, University of Minnesota

Study of human pancreas donors characteristics to obtain high islet yield and dithizone scoring system

2006-2008 Shuichiro Matsumoto, M.D., Ph.D., Visiting Post-doctoral Fellow, University of Minnesota Developing optimal porcine pancreas procurement techniques to enhance islet yield

2007-2009 Takayuki Anazawa, M.D., Ph.D., Post-doctoral Research Associate, University of Minnesota Methods to improve islet yield and purification recovery from chronic pancreatitis pancreases

2009-2010 Takeshi Yuasa, M.D., Ph.D., Research Associate, Schulze Diabetes Institute, University of Minnesota

Improving Porcine Islet Isolation and yields 2007-2013 Mukesh Tiwari, M.D., Research Associate, Schulze Diabetes Institute, University of Minnesota

Improving islet yield and transplant outcome after islet auto transplantation 2007-2014 Gopalakrishnan Loganathan, M.S., Research Fellow, Schulze Diabetes Institute, University of Minnesota

Role of acinar cells on islet isolation outcome.

Clinical Endocrinology Fellow

2014- 2016 Dr. Manikya Kuriti. M.D., Clinical Fellow of Endocrinology, University of Louisville. “Effect of pancreas preservation condition and cold ischemia time on islet yield and function”

Medical School Student

2016- Present Miss. Miranda Schmidt, medical student, University of Louisville School of Medicine. (NIDDK T35

Training Grant) Ductal neogenesis of beta cells

Training activities for International Visitors

2001-2006 Taught the process of “Human Islet Isolation” to international visitors at the Thomas E. Starzl

Transplantation Institute of University of Pittsburgh, Pittsburgh, PA 2006-2014 Trained 25 international visitors on “Autologous islet cell process of chronic pancreatitis pancreas” at

the Schzule Diabetes Institute of University of Minnesota, Minneapolis, MN CLINICAL ACTIVITIES

As the Director of Clinical Islet Cell Laboratory, I isolate islet cells following FDA regulatory guidelines from chronic pancreatitis pancreas in a clean room GMP facility. We test the quality of islets and sterility of the product and infuse the islets to patients. We performed 9 clinical islet cell auto transplantations during the year 2015. As of September 2016, 13 clinical transplantations have been performed.


We have processed more than 45 brain dead donor human pancreases towards the establishment of clinical islet cell allo-transplantation.

Other Key Activities and Accomplishments

1. Organizing Committee: 2016 American Transplant Congress: Luncheon Workshop: Islet Auto-transplantation

following Total Pancreatectomy and Outcomes. Boston, May 11-15, 2016.

2. Co-Chairman: Oral Session: Topics in Experimental and Clinical Islet Transplantation; 2015 Joint Congress of the

International pancreas and islet transplantation association (IPITA-IXA-CTS held in Melbourne, Australia, 15–19 November 2015.

3. 2015 IPITA-IXA-CTS Best Young investigators Scientific award to Mr. Gopalakrishnan Loganathan (my research associate) for our work “Stimulation of human intraislet endothelial cells yields peri-islet capillaries: Role of adipose-derived stromal vascular fraction cells and ECGS solution” (Gopalakrishnan Loganathan, Venkat M Ramakrishnan, Nolan L Boyd, Jason E Beare, Benjamin Tweed, Leigh Kleinert, Michael J Hughes, James B Hoying, Stuart K Williams, Balamurugan AN).

4. Invited Consultant: Australia’s first islet auto-transplantation at the Royal Adelaide Hospital, University of Adelaide, Australia, July 14, 2015

5. Invited Supervisor: First clinical islet auto-transplantation process at the George Town University Hospital, Washington DC. May 27, 2015.

6. Scientific Organizing Committee Member: TRENDO 2015- Emerging Trends in Endocrinology and Diabetes. 3rd Annual Conference of Endocrine Society of Tamilnadu and Puducherry, Kodaikanal, TN, India, July 11-12, 2015.

7. Organizing Committee: 2015 American Transplant Congress: Luncheon Workshop: Total Pancreatectomy and Islet Autotransplantation, Philadelphia, May 2015.

8. Co-Chairman: Stem cell- parallel session: The 14th Congress of the Middle East Society for Organ Transplantation, Istanbul, Turkey, September 2014.

9. Organizing committee member: NIDDK organized “Total Pancreatectomy with Islet Auto-Transplantation (TP-IAT): Gaps, Needs, and Opportunities” Workshop, Pittsburgh, PA, July 2014.

10. Co-Chairman: Oral presentations on Tissue Engineering: The Joint Congress of International Xenotransplantation Association and Organ Transplantation in ABO-incompatible and hyperimmunized recipients, Osaka, Japan, November, 2013.


11. Chairman: Workshop on “Clinical and Basic Research on Pancreas and Islet Transplantation” 13th Congress of the Asian Society of Transplantation (CAST 2013), Kyoto, Japan, September 2013.

12. Chairman: Oral presentation session on islets, Monday, July 8. 12th Congress of the Cell Transplant Society, July 7-11, 2013, Milan, Italy.

13. Moderator: Guided Audio Poster Tour Moderator at the Association’s 73rd Scientific Sessions of American Diabetes Association Meeting, June 21-25, 2013, Chicago, Illinois. Novel Approaches to Islet Engraftment and Survival after Transplantation (Monday, June 24, 2013).

14. Organizing Chairman of USA region: First Indo-US Bilateral workshop on “Pancreatic Islets –from isolation to transplantation” held in Hyderabad, India on September 14-15, 2012.

15. Chairman: Parallel Session1 (Cell Track) Islet; Cell Transplantation Society-International Xenotransplantation Association 2011 Joint Congress, Miami, 23-26, 2011.

16. Chairman: Islet manufacturing committee early morning concurrent session; Clinical Islet Transplant Consortium Steering Committee Face- to- Face Meeting, April 5, 2011, Arlington, VA.

17. Chairman: Joint International Pancreas and Islet Transplantation Association –International Xenotransplantation Association meeting Parallel Session 7. Pancreas procurement/Islet isolation 2. IPITA-IXA Joint Meeting, Venice, Italy; October 13, 2009.

18. Co-Chairman: Session-II, Collagenase: National Islet Cell Resource Center’s 4th annual Islet Workshop, Newport Beach, CA; October 3, 2008.

19. Co-Chairman: Concurrent Oral Session 9: Xenotransplantation - Coagulation/Islets. 22nd International Congress of The Transplantation Society, Sydney, Australia; August 11, 2008.

20. Chairman: State-of-the-Art Symposium Concurrent Session 36: Novel Treatment Paradigms for Islet Transplantation. 22nd International Congress of The Transplantation Society, Sydney, Australia; August 14, 2008.

GRANTS AND CONTRACTS

Current

Ductal Cells of Human Chronic Pancreatitis Pancreas for Islet Beta Cell Neogenesis. Jewish Heritage Fund for Excellence (Balamurugan Appakalai, Principal Investigator) $299,909. (11/1/15-10/31/17)


Establishment of islet cell autotransplantation program in University of Louisville. Jewish Heritage Fund for Excellence (Balamurugan Appakalai, Co-Principal Investigator) $800,000. (6/1/14-5/31/17)

Submitted Grants:

RO1 submitted June 2015: Balamurugan (Principal Investigator), Stimulation of peri-islet capillaries for islet

revascularization

JDRF innovative grant submitted July 2015: Balamurugan (Principal Investigator), Peri-islet vessels formation

from human islets

Past

Alloantigen delivery via ECDI-fixed cells for tolerance to monkey islet grafts, National Institutes of Health, Co-

Investigator (Dr. Bernhard Hering, Principal Investigator), $638,806, 07/01/12 – 06/30/17 (direct cost, annual

project period)

Ex vivo expansion of pancreatic islet beta cells, Juvenile Diabetes Research Foundation Genomic Institute, Co-

Investigator (Dr. Bernhard Hering, Principal Investigator), $159,030, 10/01/12 – 03/31/13 (direct cost, annual

project period)

Transgenic pig islet xenografts in non-human primates, Iacocca Family Foundation, Co-Investigator (Dr.

Bernhard Hering, Principal Investigator), $300,000, 11/01/12 – 10/30/14 (direct cost, annual project period)

Pig to Non-Human Primate Xenografts, National Institute of Allergy and Infectious Diseases, U19 AI067151, Co-

Investigator (Dr. Bernhard Hering, Principal Investigator), 07/01/08 - 06/30/10

Pig to Non-Human Primate Islet Xenografts, National Institute of Allergy and Infectious Diseases, U19 AU067151,

Co-Investigator (Dr. Bernhard Hering, Principal Investigator), 07/01/08 - 06/30/10

JDRF Islet Transplantation Center at the Univ. of Minn, Efalizumab (Raptiva) Combined with Sirolimus in Type 1

diabetic Islet Allograft Recipients, Juvenile Diabetes Research Foundation, 4-2008-386, Co-Investigator (Dr.

Bernhard Hering, Principal Investigator), 02/01/08 - 01/31/10

JDRF Pig to Non-Human Primate Islet Xenotransplantation - Transplantation in Non-Human Primates, Juvenile

Diabetes Research Foundation, 21-2006-88, Co-Investigator (Dr. Bernhard Hering, Principal Investigator),

01/01/07 - 12/31/09

Human Pancreatic Islets Cell Resources Center, National Center for Research Resources, U42 RR016599 ICR

Consortium, Co-Investigator (Dr. Ronald Gill, Principal Investigator), 2006-2007

Human Beta Cell Parameters for Islet Engraftment Success, National Institute of Diabetes and Digestive and

Kidney Diseases, R21 DK068833, Co-Investigator (Dr. Peter Drain, Principal Investigator), 07/01/05 – 06/30/06

Alpha 1,3 GT DKO pigs as donors for xenogeneic islet transplants, Juvenile Diabetes Research Foundation, Co-

Investigator (Dr. Massimo Trucco, Principal Investigator), 2004-2008


Advancing Islet Transplants for Type 1 Diabetes Care, National Institute of Allergy and Infectious Diseases, U01

AI65193, Co-Investigator (Dr. Bernhard Hering, Principal Investigator), 09/30/04 - 07/31/10

hOKT3y1 (Ala Ala), Sirolimus and Low Dose Tacrolimus Therapy in Islet Allograft Recipients, Juvenile Diabetes

Research Foundation, 4-2004-372, Co-Investigator (Dr. Bernhard Hering, Sub-Contract Principal Investigator),

02/01/04 - 01/31/09

Gene modified cloned pigs, National Institute of Diabetes and Digestive and Kidney Diseases, R01 DK064207, Co-

Investigator (Dr. Thomas Starzl, Principal Investigator), 06/01/03 – 03/31/06

Human Pancreatic Islet Cell Resource at the University of Minnesota, National Center for Research Resources,

U42 RR16598, Co-Investigator (Dr. Bernhard Hering, Principal Investigator), 09/01/01 - 07/31/09

Tolerance to Islet Allografts in Type 1 Diabetes, National Institute of Diabetes and Digestive and Kidney Diseases,

R01 DK56963, Co-Investigator (Dr. Bernhard Hering, Principal Investigator), 09/30/00 - 07/31/09

Gene Therapy Approaches to Type I Diabetes, Juvenile Diabetes Research Foundation, Co-Investigator (Dr.

Massimo Trucco, Principal Investigator), 10/01/99 - 09/30/04

Islet Biology and Bio-artificial pancreas transplantation, JSPS post-doctoral fellowship grant, Government of

Japan, Japan (Balamurugan Appakalai, Principal Investigator) 04/01/1999-12/30/200

Culture and transplantation of isolated monkey pancreatic islets, Department of Science and Technology, India. Role: Senior Research Fellow (Dr. S. Gunasekaran, PI) 10/06/1993-10/06/1995.

PATENTS N/A EDITORIAL WORK 2012-2013 Editor-in-Chief, International Journal of Endocrinology:

Special issue on Islet cell biology, regeneration & transplantation 2011- Present Member, Editorial Board, World Journal of Diabetes 2012- Present Member, Editorial Board, World Journal of Gastroenterology 2012- Present Member, Editorial Board, Science Journal of Clinical Medicine 2014- Present Member, Editorial Board, Journal of Diabetes Research & Therapy 2014- Present Member, Editorial Board, J of Transplantation Technologies & Research

ORAL PRESENTATIONS


Peer-reviewed Oral Presentations at Professional Meetings, Conferences, etc.

1. Balamurugan A.N: Improving porcine islet yield with new class 1, class 2 collagenases (mammalian tissue free) in combination with dispase. The Joint Congress of International Xenotransplantation Association and Organ Transplantation in ABO-incompatible and hyperimmunized recipients, Osaka, Japan, November, 2013

2. Balamurugan. A.N. Efficacy of new mammalian tissue free class 1 and class 2 collagenases in combination with dispase in porcine islet processing. Autoislet cell therapy product manufacturing with intact versus truncated collagenase I and II combined with netural protease. Pretreatment of donor pig islets with diet rich in soybean oil increases the yield of isolated porcine islets. Allogenic pancreas for islet donor assessment of high islet product transplant rate and donor pancreas utilization in a Phase III clinical trial. 14th World Congress of the International Pancreas and Islet Transplant Association, September 2013, Monterey, CA.

3. Tiwari M., Wilhelm J., Gopalakrishnan L., Bellin M., Soltani S., Beilman G., Sutherland D., Hering B., Balamurugan A.N. Improved enzyme dosing protocol to increase islet yield in chronic pancreatitis pancreas intended for clinical islet auto-transplantation. 24th International congress of The Transplantation Society, July 2012, Berlin, Germany.

4. Balamurugan A.N., Soltani S., Bellin M., Sutliff R., Loganathan G., Papas K., Hering B., Sutherland D. Naturally pure islets: Islet isolation from chronic pancreatitis pancreases for islet auto-transplantation. 2011 Joint Congress of the Cell Transplantation Society-International Xenotransplantation Association, October 2011, Miami, Florida.

5. Balamurugan A.N., Loganathan G., Bellin M., Wilhelm J., Harmon J., Soltani S., Tiwari M., Papas K., McCarthy R., Sutherland D., Hering B. New enzyme mixture consistently achieves higher islet yield for human islet auto- and allo-transplantations. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

6. Loganathan G., Tian L., Gao J., Guo Z., Sanders M., Dawra R., Saluja A., Sutherland D., Hering B., Balamurugan A.N. Insulin molecule cleavage and delayed human islet function after transplantation due to proteases released from co-transplanted pancreatic acinar cells” 70th Annual American Diabetes Association Meeting, June 2010, Orlando, Florida.

7. Balamurugan A.N., Jing H., Fengli G., Donna S., Suzanne B., Xuehui G., Xinhui G., Massimo T., Bottino. Harmful Delayed Effects of Exogenous Isolation Enzymes on Isolated Human Islets: Relevance to Clinical Transplantation. 10th World Congress of the International Pancreas and Islet Transplantation Association, May 2005, Geneva, Switzerland.

8. Balamurugan A.N., Gu Y., Tabata Y., Miyamoto M., Cui W., Hori H., Satake A., Nagata N.,Wang W., Inoue K. Survival and Function of Rat Islets at Prevascularized Intermuscular Site – Effect of Angiogenesis Induction. XVIII International Congress of the Transplantation Society, August 2000, Rome, Italy.

9. Balamurugan A.N., Nelson E.J., Ramakrishna B., Gunasekaran S. Xenographic Survival and Function of Transplanted Monkey Pancreatic Islets in Rats. 5th Congress of the International Xenotransplantation Association, October 1999, Nagoya, Japan.

10. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Amelioration of Stretozotocin- induced Diabetes in Rats by Xeno Transplantation of Monkey Pancreatic Islets. 43rd National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1997, Lucknow, Utar Pradesh, India.

11. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Isolation, Culture and Transplantation of Monkey Pancreatic Islets. 18th National Annual Conference of the Indian Association of Biomedical Scientists, November 1997, DIPAS, Delhi.


12. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Insulin Secretory Characteristics of Cultured Monkey Pancreatic Islets"– presented this paper at the 42nd National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1996, Cuttack, Orissa, India.

13. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Effect of Cyclosporine-A on Xenotransplantation of Monkey Pancreatic islets into rats. 41st National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1995, Belgaum, Karnataka, India.

14. Balamurugan A.N., Gunasekaran S. Comparison of Three Discontinuous Density Gradients on the Yield of Monkey Pancreatic Islets for Transplantation. 40th National Annual Conference of Association of Physiologists and Pharmacologists of India, November 1994, Kolar, Karnataka, India.

Poster Abstract Presentations at Professional Meetings, Conferences, etc.

1. 2014 World Transplant Congress, San Francisco July 26-31, 2014. Balamurugan AN, G Loganathan, M Tiwari, S Soltani, J Ansite, T Gilmore and Bernhard Hering. Achieving High

Porcine Islet Yield with Class 1, Class 2 Collagenases (Mammalian Tissue Free) in combination with Dispase.

2. Balamurugan AN, ML Green, A Breite, G Loganathan, J Wilhelm, L Vargova, A Lockridge, BJ Hering, FE Dwulet, RC McCarthy. Late breaking abstract: Influence of different ratios of purified class I and class II recombinant C. histolyticum collagenase on human pancreas digestion and islet yields when using with purified P. polymyxa protease enzyme mixtures.

3. Tiwari M, Wilhelm J, Bellin M, Soltani S, Loganathan G, Dunn T, Chinnakotla S, Vickers S, Pruett T, Beilman G, Sutherland D, Hering B, Balamurugan A.N. Predicting Digest Tissue Volume of Chronic Pancreatitis Pancreas Intended for Islet Auto-Transplantation (IAT): Correlations of In Vitro Islet Potency Tests with Clinical Islet Auto-Transplant Outcome:

Intraportal Autoislet Cell Transplantation (IAT): Changes in Portal Pressure and Flow and Associated Clinical

Outcomes

2013 American Transplantation Congress, May 2013, Seattle, WA.

4. Balamurugan A.N.: Pancreatic exocrine tissue mass affects basal insulin accumulation: Studies on islet auto-transplant preparations with Alpha-1 antitrypsin (A1AT) supplementation. Rachmiel Levine Diabetes and Obesity Symposium at The Langham Huntington, Pasadena, March 2013, CA.

5. Tiwari M., Gopalakrishnan L., Soltani S., Bellin M., Wilhelm J., Beilman G., Sutherland D., Hering B., Balamurugan A.N. Islet isolation experience from cystic fibrosis pancreatic lipomatosis case intended for islet auto-transplantation. 2012 Joint meeting of American Pancreatic Association and International Association of Pancreatology, November 2012, Miami, Florida.

6. Wilhelm J., Bellin., Perrault B., Beilman G., Pruett T., Balamurugan A.N., Papas K., Hering B. Oxygen consumption predicts insulin usage in patients undergoing total pancreatectomy and islet autotransplantation (TP-IAT). 2012 Joint meeting of American Pancreatic Association and International Association of Pancreatology, November 2012, Miami, Florida.


7. Gopalakrishnan L., Soltani S., Tiwari M., Bellin M., Wilhelm J., Beilman G., Sutherland D., Hering B., Balamurugan A.N. Islet isolation outcome from patients with chronic pancreatitis (CP) and c-peptide positive diabetes mellitus (DM) undergoing total pancreatectomy for islet auto-transplantation. 2012 Joint Meeting of American Pancreatic Association and International Association of Pancreatology, November 2012, Miami, Florida.

8. Balamurugan A.N. Methods to maximizing islet yield and quality in chronic pancreatitis pancreas intended for islet auto-transplantation utilizing a new enzyme mixture and Alpha-1 antitrypsin. Indo-US bilateral workshop on Pancreatic islets, September 2012, Hyderabad, India.

9. Soltani S., Gopalakrishnan L., Wilhelm J., Tiwari M., Bellin M., James H., Sutherland D., Hering B., Balamurugan A.N.

Human islet isolation for clinical transplantation: University of Minnesota with different islet isolation protocols and

isolation enzymes. 24th International congress of The Transplantation Society, July 2012, Berlin, Germany.

10. Tiwari M., Wilhelm J., Gopalakrishnan L., Soltani S., Bellin M., Beilman G., Sutherland D., Hering B., Balamurugan A.N. Improved enzyme dosing protocol to increase islet yield in chronic pancreatitis pancreas intended for clinical islet auto-transplantation. 24th International congress of The Transplantation Society, July 2012, Berlin, Germany.

11. Gopalakrishnan L., Wilhelm J., Soltani S., Tiwari M., Bellin M., Beilman G., Sutherland D., Hering B., Balamurugan A.N. Beneficial effect of Alpha-1 antitrypsin supplementation to unpurified human islets: Studies on clinical islet auto-transplant preparations. 72nd American Diabetes Association (ADA), June 2012, Philadelphia, Pennsylvania.

12. Bellin M., Chinnakotla S., Beilman G., Sutherland D., Hering B., Balamurugan A.N., Dunn T., Pruett T. Islet autotransplant to prevent diabetes in children undergoing total pancreatectomy for chronic pancreatitis. 72nd American Diabetes Association (ADA), June 2012, Philadelphia, Pennsylvania.

13. Bellin M., Beilman G., Balamurugan A.N. Chinnakotla S., Dunn T., Pruett T., Sutherland D., Hering B. Insulin independence after islet autotransplant is associated with subtle differences in early post-transplant glycemic control. 72nd American Diabetes Association (ADA), June 2012, Philadelphia, Pennsylvania.

14. Balamurugan A.N., Wilhelm J., Gopalakrishnan L., Bellin M., Beilman G., Chinnakotla S., Dunn T., Pruett T., Vickers S., Hering B., Sutherland D. Key approaches to maximizing islet yield from pancreata with chronic pancreatitis for use in islet auto-transplantation. 12th Annual Rachmiel Levine Diabetes and Obesity Symposium, February 2012, Pasadena, California.

15. Balamurugan A.N., Wilhelm J., Gopalakrishnan L., Bellin M., Beilman G., Chinnakotla S., Dunn T., Pruett T., Vickers S., Hering B., Sutherland D. Maximizing Islet Yield from Pancreata with Chronic Pancreatitis for Use in Islet Auto-Transplantation Requires a Modified Strategy from Islet Allograft Preparations. 42nd Annual meeting of the American Pancreatic Association, November 2011, Chicago, Illinois. Poster of Distinction Award.

16. Wilhelm J., Bellin M., Dunn T., Balamurugan A.N., Pruett T., Radosevich D., Chinnakotla S., Hering B., Sutherland D., Beilman G. A Proposed Threshold for Dispersed-Pancreatic-Tissue-Volume (TV) Infused During Intraportal-Islet-Autotransplantation(IAT) after Total-Pancreatectomy(TP) to Treat Chronic-Pancreatitis(CP). 42nd Annual meeting of the American Pancreatic Association, November 2011, Chicago, Illinois.


17. Soltani S., Gopalakrishnan L., Melina B., Papas K., Dunn T., Vickers S., Beilman G., Hering ., Sutherland D., Balamurugan A.N. Naturally pure islets: Islet isolation from chronic pancreatitis pancreases for islet auto-transplantation. 2011 Joint Congress of the Cell Transplantation Society-International Xenotransplantation Association, October 2011, Miami, Florida.

18. Scott W., Weegman B., Balamurugan A.N., Anazawa T., Hammer B., Avgoustiniatos S., Sutherland D., Hering B., Papas K. Shipping of Juvenile Porcine Islets in Silicon Rubber Membrane (SRM) Vessels. 2011 Joint Congress of the Cell Transplantation Society-International Xenotransplantation Association, October 2011, Miami, Florida.

19. Mueller K., Balamurugan A.N., Cline G., Pongratz R., Hooper L., Weegman P., Kitzmann P., Taylor J., Graham M, Schuurman H.,, Papas K. Comparison of glucose-stimulated insulin secretion between isolated human, nonhuman primate and porcine islets. 2011 Joint Congress of the Cell Transplantation Society-International Xenotransplantation Association, October 2011, Miami, Florida.

20. Balamurugan A.N., Gopalakrishnan G., Bellin M., Wilhelm J., Harmon J., Soltani S., Tiwari M., Papas K., McCarthy R., Sutherland D., Hering B. New enzyme mixture consistently achieves higher islet yield for human islet auto- and allo-transplantations. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

21. Abouaish J., Graham M., Pakala P., Gopalakrishnan G., Tiwari M., Yuasa T., Papas K., Sutherland D., McCarthy R., Hering B., Balamurugan A.N. Successful isolation and transplantation of non-human primate islets utilizing collagenase and thermolysin purified enzymes. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

22. Mukesh T., Brian F., Harmon J., Wilhelm J, Gopalakrishnan L., Sutherland D., Hering B., Balamurugan A.N. Factors determining the acceptance of deceased donor pancreases for human islet transplantation. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

23. Soltani S., Gopalakrishnan L., Melina B., Papas K., Dunn T., Vickers S., Beilman G., Hering ., Sutherland D., Balamurugan A.N. Reducing the transplantable tissue volume for human islet auto-transplantation: A high density purification process different from islet allograft purification. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

24. Anazawa T., Graham M., Abouaish J., Gopalakrishnan L., Hering B., Balamurugan A.N. Predicting porcine islet yield by acute C-peptide response to arginine stimulation of donor pigs and pancreas dithizone scoring system. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

25. Janine A., Brien T., Parker J., Graham M., Soltani M, Gopalakrishnan L., Hering B., Balamurugan A.N. Islet area fraction measurements in 236 porcine pancreas biopsies to identify suitable pigs for islet isolation. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.


26. Gopalakrishnan L., Dawra R., Pugazhenthi S., Guo Z., Soltani S., Wiseman A., Sanders M., Papas K., Saluja K., Sutherland D.,. Hering B., Balamurugan A.N. Acinar cell proteases reduce insulin granule in vitro and degrade insulin after transplantation: Preventive effect of Alpha-1 antitrypsin. 13th Congress of the International Pancreas and Islet Transplant Association (IPITA), June 2011, Prague, Czech Republic.

27. Balamurugan A.N., Gopalakrishnan L., Bellin M., Papas K., Dunn T., Vickers S., Beilman G., Hering B., Sutherland D.

High islet yield from pre-teenage pediatric patients undergoing total pancreatectomy and islet autotransplantation for chronic pancreatitis compared to teen-age and adult patients. 71st Annual American Diabetes Association Meeting, June 2011, San Diego, California.

28. Balamurugan A.N., Gopalakrishnan L., Bellin M., Wilhelm J., Yuasa T., Tiwari M., Papas K., Vickers S., Beilman G., Hering B., Sutherland D. Islet Isolation Outcome from Minimal Change Chronic Pancreatitis Is Comparable with Clinical Grade Allograft Pancreases. 12th American Transplant Congress held at the Pennsylvania Convention Center, May 2011, Philadelphia, Pennsylvania.

29. Balamurugan A.N., Gopalakrishnan L., Bellin M., Wilhelm J., Harmon J., Aanazawa T., Radosevich D., Yuasa T., Tiwari M., Papas K., McCarthy R., Sutherland D., Hering B. A New Enzyme Mixture to Consistently Achieve High Human Islet Yield and Improved Allogenic and Autograft Islet Transplantation Outcome. XXIII International Congress of The Transplantation Society, August 2010, Vancouver, Canada.

30. Balamurugan A.N., Brien T., Gopalakrishnan L., Bellin M., Anazawa T., Papas K., Vickers S., Hering B., Sutherland D. Severely fibrotic pancreata from young patients with chronic pancreatitis: Evidence for ductal neogenesis of islets. XXIII International Congress of The Transplantation Society, August 2010, Vancouver, Canada.

31. Loganathan L., Radosevich, D., Anazawa T., Graham M., Papas K., Sutherland D., Hering., Balamurugan A.N. Factors affecting transplant outcome in diabetic nude mice receiving human, porcine, and non-human primate islets: Lessons from 328 transplantations. XXIII International Congress of The Transplantation Society, August 2010, Vancouver, Canada.

32. Gopalakrishnan L., Dawra R., Pugazhenthi S., Guo Z., Soltani S., Wiseman A., Sanders M., Papas K., Saluja K., Sutherland D.,. Hering B., Balamurugan A.N. Insulin molecule cleavage and delayed human islet function after transplantation due to proteases released from co-transplanted pancreatic acinar cells. 70th Annual ADA-American Diabetes Association Meeting, June 2010, Orlando, Florida.

33. Balamurugan A.N., Gopalakrishnan L., Graham M., Pakala., Yuasa T., Papas K., Sutherland D., McCarthy R., Hering B. Successful isolation and transplantation of non-human primate islets utilizing VitaCyte CIzyme™ collagenase MA and

CIzyme thermolysin purified enzymes. 70th Annual ADA-American Diabetes Association Meeting, June 2010, Orlando, Florida.

34. Balamurugan A.N., Gopalakrishnan L., Wilhelm J., Yuasa T., Radosevich D., Papas K., Bellin M.,. Beilman G., McCarthy R., Sutherland D., Hering B. Improved islet yield from diseased pancreases for autotransplantation using a new enzyme mixture. 11th American Transplant Congress, May 2010, San Diego, California.


35. Gopalakrishnan L., Tian L., Gao J., Guo Z., Dawra R., Saluja K., Sutherland D., Hering B., Balamurugan A.N. Degradation of insulin by acinar cell proteases results in a dysfunctional environment for islets before and after transplantation: Beneficial effect of alpha-1 antitrypsin treatment. 10th Annual Rachmiel Levine Diabetes and Obesity Symposium, March 2010, Las Vegas, California.

36. Balamurugan A.N., Gopalakrishnan L., Wilhelm J., Yuasa T., Radosevich D., Papas K., Bellin M.,. Beilman G., McCarthy R., Sutherland D., Hering B. Synergetic effect of purified collagenase and neutral protease combination maximizes human islet yield for transplantation and alpha-1 antitrypsin supplementation improves islet culture recovery. 10th Annual Rachmiel Levine Diabetes and Obesity Symposium, March 2010, Las Vegas, California.

37. Balamurugan A. N., Lewis E. C., Wiseman A. C., Pugazhenthi S., Sarkar S. A., Dinarello C. A., Gill R. G. Effect of in vitro treatment of isolated human islets with human alpha-1 antitrypsin. 22nd International Congress of The Transplantation Society, August 2008, Sydney, Australia.

38. Anazawa T., Matsumoto S., Yonekawa Y., Gopalakrishnan L., Wilhelm J., Papas K., Sutherland D.,Hering B., Balamurugan A.N. Analytical/Continuous Test Gradient System [mini COBE] for prediction of acinar cell density and improved purification of human islets. 9th American Transplant Congress held at June 2008, Boston, Massachusetts.

39. Gopalakrishnan L., Balamurugan A.N., Dawra R., Anazawa T., Wiseman A., Pugazhenthi S., Papas K., Saluja K., Sutherland D., Hering B., New Approach for Impure Human Islet Culture before Transplantation: Effect of alpha-1 antitrypsin and Protandim supplementation. 9th American Transplant Congress held at June 2008, Boston, Massachusetts.

40. Anazawa T., Balamurugan A.N., Bellin M., Zhang H., Matsumoto S., Yonekawa Y., Tanaka T., Papas K., Beilman G., Hering B., Sutherland D. Liberase-HI and Serva-NB1 plus NB enzymes are equally effective for human islet autograft. 8th American Transplant Congress, June 2008, Toronto, Ontario, Canada.

41. Yonekawa Y., Zhang H., Matsumoto S., Tanaka T., Anazawa T., Ansite J., Gilmore T., Balamurugan A.N., Hering B., Sutherland D. Microbial Contamination for Islet Autotransplantation. 8th American Transplant Congress, June 2008, Toronto, Ontario, Canada.

42. Sutherland D., Gruessner A., Carlson A., Balamurugan A.N., Ansite J., Gilmore T., Matsumoto S., Yonekawa Y., Tanaka T., Hering B. Minimum islet mass required to achieve and maintain insulin independence after total pancreatectomy and islet autotransplantation. The Cell Transplantation Society-International Pancreas Islet Transplantation Association-International Xenotransplantation Association-2007 Joint Conference, September 2007, Minneapolis, Minnesota.

43. Yonekawa Y., Balamurugan A.N., Matsumoto S., Tanaka T., Gilmore T., Ansite J., Zhang H., Sutherland D., Hering B. The use of test gradients to determine the bottom layer density for subsequent continuous isopycnic purification of human islets on a COBE 2991 cell processor. The Cell Transplantation Society-International Pancreas Islet Transplantation Association-International Xenotransplantation Association-2007 Joint Conference, September 2007, Minneapolis, Minnesota.

44. Bottino R., Balamurugan, A.N., Hara H., Van der Windt D., Campanile N., Smetanka C., Cooper D., Trucco M.


Metabolic aspects of a pig-to-monkey islet xenotransplantation model: Implications for translation into clinical practice. 67th Annual American Diabetes Association Meeting, June 2007, Chicago, Illinois.

45. Suzanne B., Bottino R., Balamurugan, A.N., Sands A., Knoll C., Ayares D., Cooper D., Trucco M. Xenotransplantation of Alpha 1, 3-Galactosyltransferase Deficient Pig Islets of Langerhans into a Mouse Model. World Transplant Congress, July 2006, Boston, MA.

46. Zhang N., Su D., Qu, S., Tse T., Bottino R., Balamurugan, A.N., Xu J., Bromberg S., Jonathan S., Henry H. Safe Induction of Diabetes in Cynomolgus Monkeys with Zanosar streptozotocin: Efficacy and Response. World Transplant Congress, July 2006, Boston, MA.

47. Zhang N., Su D., Qu, S., Tse T., Bottino R., Balamurugan, A.N., Xu J., Bromberg S., Jonathan S., Henry H. Sirolimus Is

Associated with Reduced Islet Engraftment and Impaired -Cell Function. 66th Annual American Diabetes Association Meeting, June 2006, Washington, DC.

48. Bottino R., Balamurugan A.N., Chang Y., Bertera S., Sands A., Trucco M. Mantle Free Human Islets of Langerhans from the Pancreata of Young Donors. 25th Workshop of the Artificial Insulin Delivery Pancreas and Islet Transplantation (AIDPIT) Study Group, February 2006, Pisa, Italy.

49. Bottino R., Balamurugan A.N., Chang Y., Bertera S., Sands A., Trucco M. Pig donor size affects porcine islet isolation yield and islet survival. The 8th Congress of the International Xenotransplantation Society, IXA2005, September 2005, Göteborg, Sweden.

50. Rood P., Bottino R., Balamurugan A.N., Smetanka C., Ayares D., Groth C., Murase N., Cooper D., Trucco M. Streptozotocin administration in young tethered monkeys – early response, dosage, and side effects. The 8th Congress of the International Xenotransplantation Society, IXA2005, September 2005, Göteborg, Sweden.

51. Xuehui G., Lehong L., Bottino R., Balamurugan A.N., Suzanne B., Erik Densmore1, Anjey S., Yigang C., Trucco M., and Drain P. Sulfonylureas Stimulate Calcium-Dependent Second Phase Insulin Secretion Independent of Plasma Membrane K-ATP Channels. 65th Annual American Diabetes Association Meeting, June 2005, Santiago, CA.

52. Balamurugan A.N., Jing H., Fengli G., Donna S., Suzanne B., Xuehui G., Xinhui G., Massimo T., Bottino. Harmful Delayed Effects of Exogenous Isolation Enzymes on Isolated Human Islets: Relevance to Clinical Transplantation.” 10th World Congress of the International Pancreas and Islet Transplantation Association, May 2005, Geneva, Switzerland.

53. Bottino R., Balamurugan A.N., Chang Y., Bertera S., Sands A., Trucco M. Innovative Approaches to Monitoring Insulin Secretion. International Group on Insulin Secretion, Servier-IGIS Symposia, March 2005, France (St. Jean-Cap-Ferrat).


54. Bottino R., Balamurugan A.N., Bertera S, Pietropaolo M., Trucco M, Piganelli J. Quelling Oxidative Stress during Human Islet Isolation Correlates with Reduced NF-kappa B-Dependent Pro-inflammatory Mediators: Implications for the Development of Footprint Assays for Transplant Success. 64th Annual American Diabetes Association Meeting, June 2004, Orlando, Florida.

55. Rehman K., Bertera S., Bottino B., Balamurugan A. N., Mai J., Mi Z, Trucco M., Robbins P. Efficient Gene Delivery to Human and Rodent Islets with Double Stranded (ds)-AAV2-Based Vectors. 7th Annual Meeting of the American Society of Gene Therapy, June 2004, Minneapolis, Minnesota.

56. Bottino R., Balamurugan A.N., Chang Y., Bertera S., Sands A., Trucco M. Proteomic Scanning For Markers Associated With Successful Islet Isolation and Maintenance. Keystone Symposia: Diabetes Mellitus; Molecular Signaling, Genes and Therapeutics, March 2004, Banff, Alberta, Canada.

57. Rehman K., Bertera S., Bottino B., Balamurugan A. N., Mai J., Mi Z, Trucco M., Robbins P. Prolongation of Allogeneic Islet Allograft Survival in Mice Following Ex Vivo Adenoviral Delivery of Soluble Type 1 TNF Receptor-Ig Fusion. 63rd Annual Scientific American Diabetes Association Meeting, June 2003, New Orleans, Louisiana.

58. Rehman K., Bottino B., Balamurugan A. N., Mai J., Mi Z, Trucco M., Robbins P. Protection of Human Islets from Cytokine-Induced Impairment by Adenoviral VEGF Gene Transfer In Vitro. The American Society of Gene Therapy 6th Annual Meeting, June 2003, Washington DC.

59. Bottino R., Balamurugan A.N., Bertera S, Pietropaolo M., Trucco M, Piganelli J. Preservation of Human Islets After Isolation Using a Metalloporphyrin Based Superoxide Dismutase Mimic. Cell Transplant Society 10th Anniversary Meeting, October 2001, Keystone, Colorado.

60. Gu Y., Tabata Y., Kawakami Y., Balamurugan A.N., Hori H., Nagata N., Satake A., Cui W., Qi M., Misawa Y., Toma M., Miyamoto M., Nozawa M., Inoue K. Development of a Method to Induce Angiogenesis at Subcutaneous Site of Diabetic Rats for Islet Transplantation. XVIII International Congress of the Transplantation Society, August 2000, Rome, Italy.

61. Balamurugan A.N., Gu Y., Tabata Y., Miyamoto M., Cui W., Hori H., Satake A., Nagata N., Wang W., Inoue K. Survival and Function of Rat Islets at Prevascularized Intermuscular Site: Effect of Angiogenesis Induction. XVIII International Congress of the Transplantation Society, August 2000, Rome, Italy.

62. Balamurugan A.N., Nelson E.J., Ramakrishna B., Gunasekaran S. Xenographic Survival and Function of Transplanted Monkey Pancreatic Islets in Rats. 5th Congress of the International Xenotransplantation Association, October 1999, Nagoya, Japan.

63. Gu Y., Tabata Y., Balamurugan A.N., Kawakami Y., Hori H., Nagata N., Cui W. Misawa Y., Toma M., Miyamoto M., Nozawa M., Inoue K. Development of a New Immuno-isolation Method for Xenotransplantation of Porcine Islets in Rats. The Fifth Congress of the International Xenotransplantation Association, October 1999, Nagoya, Japan.


64. Balamurugan A.N., Nelson E.J., Ramakrishna B., Gunasekaran S. Effect of Various Immunosuppressive Drugs on Xenotransplantation of Monkey Pancreatic Islets into Rats. International Congress of the Sixth Asian Society of Transplantation, September 1999, Singapore.

65. Kawakami Y., Iwata H., Gu YJ., Miyamoto M., Murakami Y., Balamurugan A.N., Imamura M., Inoue K. Effect of Neovascularization-inducing Bioartificial Pancreas on Survival of Syngeneic Islet Graft. International Congress of the Sixth Asian Society of Transplantation, September 1999, Singapore.

66. Gu Y., Cui W., Miyamoto M., Kawakami Y., Xu B., Balamurugan A.N., Nagata N., Morimoto Y. Imamura M., Satake A., Iwata H., Nozawa M., Inoue K. Development of a New Bio-artificial Pancreas

Possessing Angiogenesis-inducing Function. International Congress of the Sixth Asian Society of Transplantation,

September 1999, Singapore.

67. Miyamoto M., Morimoto Y., Balamurugan A.N., Kawakami Y., Gu Y., Satake A., Suzuki Y., Tanioka Y., Kuroda Y., Shimizu N., Tohyama T., Nozawa Y., Nozawa M. Inoue K.Effectiveness of Modified Two Layer Preservation Method (PFC / Kyoto solution) in Islet Isolation of Breeder Pigs. International Congress of the Sixth Asian Society of Transplantation, September 1999, Singapore.

68. Mythili D., Balamurugan A.N., Ramakrishna B., Gunasekaran S. Electron Microscopic Studies on Isolated Monkey Pancreatic Islets used for Transplantation. 26th National Annual Conference of Research Society for the study of Diabetes in India, December 1998, Ahmedabad, Gujarat, India.

69. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Amelioration of Stretozotocin- induced Diabetes in Rats by Xeno Transplantation of Monkey Pancreatic Islets. 43rd National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1997, Lucknow, Utar Pradesh, India.

70. Gunasekaran S., Balamurugan A.N., Ramakrishna B. Xenotransplantion of Primate Pancreatic Islets - Possibility of IDDM to cure? National Annual Silver Jubilee Conference of Research Society for the study of Diabetes in India, December 1997, Madras, India.

71. Nelson E.J., Balamurugan A.N., Gunasekaran S. Does Diabetes Mellitus Occur Naturally in Wild Indian Bonnet Monkeys? National Annual Silver Jubilee Conference of Research Society for the study of Diabetes in India, December 1997, Madras, India.

72. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Isolation, Culture and Transplantation of Monkey Pancreatic Islets. 18th National Annual Conference of the Indian Association of Biomedical Scientists, November 1997, DIPAS, Delhi.

73. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Insulin Secretory Characteristics of Cultured Monkey Pancreatic Islets. 42nd National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1996, Cuttack, Orissa, India.


74. Gunasekaran S., Balamurugan A.N.Culture of Monkey Pancreatic Islets. 24th National Annual Conference of Research Society for the study of Diabetes in India, November 1996, Mysore, Karnataka, India.

75. Balamurugan A.N., Nelson E.J., Ramakrishna B., Gunasekaran S. Effect of Cyclosporine-A on Xenotransplantation of Monkey Pancreatic islets into rats. 41st National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1995, Belgaum, Karnataka, India.

76. Gunasekaran S., Balamurugan A.N. Insulin Secretory Characteristics of Monkey Pancreatic Islets Isolated on a Large Scale for Transplantation. 23rd Annual Scientific Meeting of Research Society for the Study of Diabetes in India, September 1995, Goa, India.

77. Balamurugan A.N., Ramakrishna B., Gunasekaran S. Comparison of Three Discontinuous Density Gradients on the Yield of Monkey Pancreatic Islets for Transplantation. 40th National Annual Conference of Association of Physiologists and Pharmacologists of India, November 1994, Kolar, Karnataka, India.

78. Jeevanandam T., Balamurugan A.N. Manonmani T., Namasivayam A. Antidiadetic Effect of Karela (bitter gourd - Momordica Charantia). 38th National Annual Conference of Association of Physiologists and Pharmacologists of India, December 1992, Delhi.

PUBLICATIONS

Articles Published in Peer-Reviewed Journals:

1997 [1] Sathya K, Balamurugan AN, Savithiri S, Gunasekaran S: Intravenous glucose tolerance test in Macaca radiata radiata. Indian journal of physiology and pharmacology 1997, 41:176-8. [2] Gunasekaran S, Balamurugan AN, Senthil M, Gnanaraj L: Xenographic transplantation of monkey pancreatic islets into rats. The Journal of the Association of Physicians of India 1997, Suppl 1:45-7. 1999 [3] Balamurugan AN, Ramakrishna B, Gunasekaran S: Effect of cyclophosphamide pretreatment and culture on insulin secretion by isolated monkey pancreatic islets (Macaca radiata radiata). Transplantation proceedings 1999, 31:2154-6. [4] Balamurugan AN, Ramakrishna B, Gunasekaran S: Optimization of culture conditions for the preservation of monkey pancreatic islets in culture. Pancreas 1999, 19:83-8. [5] Srivastava A, Nair SC, Srivastava VM, Balamurugan AN, Jeyaseelan L, Chandy M, Gunasekaran S: Evaluation of uroprotective efficacy of amifostine against cyclophosphamide induced hemorrhagic cystitis. Bone marrow transplantation 1999, 23:463-7. 2000


[6] Miyamoto M, Morimoto Y, Nozawa Y, Balamurugan AN, Xu B, Inoue K: Establishment of fluorescein diacetate and ethidium bromide (FDAEB) assay for quality assessment of isolated islets. Cell transplantation 2000, 9:681-6. [7] Gu YJ, Cui WX, Miyamoto M, Kawakami Y, Xu BY, Balamurugan AN, Nagata N, Morimoto Y, Imamura M, Satake A, Iwata H, Nozawa M, Inoue K: Development of a new bioartificial pancreas possessing angiogenesis-inducing function. Transplantation proceedings 2000, 32:2475. [8] Miyamoto M, Morimoto Y, Balamurugan AN, Kawakami Y, Gu Y, Satake A, Suzuki Y, Tanioka Y, Kuroda Y, Shimizu N, Tohyama T, Nozawa Y, Nozawa M, Inoue K: Improvement of modified two-layer preservation method (PFC/Kyoto solution) in islet isolation from breeder pigs. Transplantation proceedings 2000, 32:1660-1. [9] Balamurugan AN, Remington EJ, Ramakrishna B, Gunasekaran S: Xenographic survival and function of transplanted monkey pancreatic islets in rats. Transplantation proceedings 2000, 32:1068. 2001 [10] Kawakami Y, Iwata H, Gu YJ, Miyamoto M, Murakami Y, Balamurugan AN, Imamura M, Inoue K: Successful subcutaneous pancreatic islet transplantation using an angiogenic growth factor-releasing device. Pancreas 2001, 23:375-81. [11] Wang W, Gu Y, Miyamoto M, Hori H, Nagata N, Balamurugan AN, Touma M, Sakurai T, Inoue K: Effect of basic fibroblast growth factor on insulin secretion from microencapsulated pancreatic islets: an in vitro study. Cell transplantation 2001, 10:465-71. [12] Hori H, Gu YJ, Nagata N, Balamurugan AN, Satake A, Morimoto Y, Wang WJ, Misawa Y, Nozawa Y, Nembai T, Miyamoto M, Nozawa M, Inoue K: Isolation, culture, and characterization of endocrine cells from 6-month-old porcine pancreas. Cell transplantation 2001, 10:459-64. [13] Gu Y, Tabata Y, Kawakami Y, Balamurugan AN, Hori H, Nagata N, Satake A, Cui W, Qi M, Misawa Y, Toma M, Miyamoto M, Nozawa M, Inoue K: Development of a new method to induce angiogenesis at subcutaneous site of streptozotocin-induced diabetic rats for islet transplantation. Cell transplantation 2001, 10:453-7. [14] Nagata N, Gu Y, Hori H, Balamurugan AN, Touma M, Kawakami Y, Wang W, Baba TT, Satake A, Nozawa M, Tabata Y, Inoue K: Evaluation of insulin secretion of isolated rat islets cultured in extracellular matrix. Cell transplantation 2001, 10:447-51. [15] Xu B, Gu Y, Miyamoto M, Balamurugan AN, Cui W, Imamura M, Iwata H, Inoue K: The influence of the anticomplement synthetic sulfonic polymers on the function of pancreatic islets: an in vitro study. Cell transplantation 2001, 10:413-7. [16] Xu B, Iwata H, Miyamoto M, Balamurugan AN, Murakami Y, Cui W, Imamura M, Inoue K: Functional comparison of the single-layer agarose microbeads and the developed three-layer agarose microbeads as the bioartificial pancreas: an in vitro study. Cell transplantation 2001, 10:403-8. [17] Miyamoto M, Balamurugan AN, Nozawa Y, Sakurai T, Xu B, Yoshimura S, Tanaka T, Tohyama T, Miyakoshi J, Inoue K: Development of a cryopreservation procedure employing a freezer bag for pancreatic islets using a newly developed cryoprotectant. Cell transplantation 2001, 10:363-71.

2002


[18] Bottino R, Balamurugan AN, Bertera S, Pietropaolo M, Trucco M, Piganelli JD: Preservation of human islet cell functional mass by anti-oxidative action of a novel SOD mimic compound. Diabetes 2002, 51:2561-7. [19] Bottino R, Trucco M, Balamurugan AN, Starzl TE: Pancreas and islet cell transplantation. Best practice & research Clinical gastroenterology 2002, 16:457-74. [20] Bottino R, Balamurugan AN, Giannoukakis N, Trucco M: Islet/pancreas transplantation: challenges for pediatrics. Pediatric diabetes 2002, 3:210-23. [21] Wang W, Gu Y, Tabata Y, Miyamoto M, Hori H, Nagata N, Touma M, Balamurugan AN, Kawakami Y, Nozawa M, Inoue K: Reversal of diabetes in mice by xenotransplantation of a bioartificial pancreas in a prevascularized subcutaneous site. Transplantation 2002, 73:122-9. 2003 [22] Bertera S, Geng X, Tawadrous Z, Bottino R, Balamurugan AN, Rudert WA, Drain P, Watkins SC, Trucco M: Body window-enabled in vivo multicolor imaging of transplanted mouse islets expressing an insulin-Timer fusion protein. BioTechniques 2003, 35:718-22. [23] Balamurugan AN, Chang Y, Fung JJ, Trucco M, Bottino R: Flexible management of enzymatic digestion improves human islet isolation outcome from sub-optimal donor pancreata. American Journal of Transplantation 2003, 3:1135-42. [24] Balamurugan AN, Gu Y, Tabata Y, Miyamoto M, Cui W, Hori H, Satake A, Nagata N, Wang W, Inoue K: Bioartificial pancreas transplantation at prevascularized intermuscular space: effect of angiogenesis induction on islet survival. Pancreas 2003, 26:279-85. [25] Rehman KK, Bertera S, Bottino R, Balamurugan AN, Mai JC, Mi Z, Trucco M, Robbins PD: Protection of islets by in situ peptide-mediated transduction of the Ikappa B kinase inhibitor Nemo-binding domain peptide. The Journal of biological chemistry 2003, 278:9862-8. [26] Balamurugan AN, Gu Y, Miyamoto M, Hori H, Inoue K, Tabata Y: Effect of hepatocyte growth factor (HGF) on adult islet function in vitro [corrected]. Pancreas 2003, 26:103-4. (Corresponding Author). [27] Balamurugan AN, Gu Y, Miyamoto M, Wang W, Inoue K, Tabata Y: Isolation, culture, and functional characteristics of diabetic islets [corrected]. Pancreas 2003, 26:102-3. (Corresponding Author). 2004 [28] Bottino R, Balamurugan AN, Tse H, Thirunavukkarasu C, Ge X, Profozich J, Milton M, Ziegenfuss A, Trucco M, Piganelli JD: Response of human islets to isolation stress and the effect of antioxidant treatment. Diabetes 2004, 53:2559-68. [29] Balamurugan AN, Ramakrishna B, Gunasekaran S: Insulin secretory characteristics of monkey pancreatic islets: a simple method of islet isolation and the effect of various density gradients on separation. Diabetes research and clinical practice 2004, 66:13-21. (Corresponding Author). [30] Machen J, Bertera S, Chang Y, Bottino R, Balamurugan AN, Robbins PD, Trucco M, Giannoukakis N: Prolongation of islet allograft survival following ex vivo transduction with adenovirus encoding a soluble type 1 TNF receptor-Ig fusion decoy. Gene therapy 2004, 11:1506-14.


[31] Cozar-Castellano I, Takane KK, Bottino R, Balamurugan AN, Stewart AF: Induction of beta-cell proliferation and retinoblastoma protein phosphorylation in rat and human islets using adenovirus-mediated transfer of cyclin-dependent kinase-4 and cyclin D1. Diabetes 2004, 53:149-59. 2005 [32] Balamurugan AN, He J, Guo F, Stolz DB, Bertera S, Geng X, Ge X, Trucco M, Bottino R: Harmful delayed effects of exogenous isolation enzymes on isolated human islets: relevance to clinical transplantation. American Journal of Transplantation2005, 5:2671-81. [33] Rehman KK, Wang Z, Bottino R, Balamurugan AN, Trucco M, Li J, Xiao X, Robbins PD: Efficient gene delivery to human and rodent islets with double-stranded (ds) AAV-based vectors. Gene therapy 2005, 12:1313-23. 2006 [34] Rood PP, Bottino R, Balamurugan AN, Smetanka C, Ezzelarab M, Busch J, Hara H, Trucco M, Cooper DK: Induction of diabetes in cynomolgus monkeys with high-dose streptozotocin: adverse effects and early responses. Pancreas 2006, 33:287-92. [35] Zhang N, Su D, Qu S, Tse T, Bottino R, Balamurugan AN, Xu J, Bromberg JS, Dong HH: Sirolimus is associated with reduced islet engraftment and impaired beta-cell function. Diabetes 2006, 55:2429-36. [36] Balamurugan AN, Chang Y, Bertera S, Sands A, Shankar V, Trucco M, Bottino R: Suitability of human juvenile pancreatic islets for clinical use. Diabetologia 2006, 49:1845-54. [37] Balamurugan AN, Bottino R, Giannoukakis N, Smetanka C: Prospective and challenges of islet transplantation for the therapy of autoimmune diabetes. Pancreas 2006, 32:231-43. (Corresponding Author). [38] Rood PP, Bottino R, Balamurugan AN, Fan Y, Cooper DK, Trucco M: Facilitating physiologic self-regeneration: a step beyond islet cell replacement. Pharmaceutical research 2006, 23:227-42. [39] D'Anneo A, Rood P, Bottino R, Balamurugan AN, He J, Giannoukakis N: Gene therapy for type 1 diabetes: is it ready for the clinic? Immunologic research 2006, 36:83-9. 2007 [40] Balamurugan AN, Nelson EJ, Ramakrishna B, Gunasekaran S: Effect of various immunosuppressive monotherapies on survival and histopathology of monkey islet xenografts in rats. Xenotransplantation 2007, 14:316-22. (Corresponding Author). [41] Geng X, Li L, Bottino R, Balamurugan AN, Bertera S, Densmore E, Su A, Chang Y, Trucco M, Drain P: Antidiabetic sulfonylurea stimulates insulin secretion independently of plasma membrane KATP channels. American journal of physiology Endocrinology and metabolism 2007, 293:E293-301. [42] Rood PP, Bottino R, Balamurugan AN, Smetanka C, Ayares D, Groth CG, Murase N, Cooper DK, Trucco M: Reduction of early graft loss after intraportal porcine islet transplantation in monkeys. Transplantation 2007, 83:202-10. [43] Bottino R, Balamurugan AN, Smetanka C, Bertera S, He J, Rood PP, Cooper DK, Trucco M: Isolation outcome and functional characteristics of young and adult pig pancreatic islets for transplantation studies. Xenotransplantation 2007, 14:74-82. 2008


[44] Sutherland DE, Gruessner AC, Carlson AM, Blondet JJ, Balamurugan AN, Reigstad KF, Beilman GJ, Bellin MD, Hering BJ: Islet autotransplant outcomes after total pancreatectomy: a contrast to islet allograft outcomes. Transplantation 2008, 86:1799-802. [45] Bellin MD, Kandaswamy R, Parkey J, Zhang HJ, Liu B, Ihm SH, Ansite JD, Witson J, Bansal-Pakala P, Balamurugan AN, Papas KK, Sutherland DE, Moran A, Hering BJ: Prolonged insulin independence after islet allotransplants in recipients with type 1 diabetes. American Journal of Transplantation2008, 8:2463-70. [46] Avgoustiniatos ES, Hering BJ, Rozak PR, Wilson JR, Tempelman LA, Balamurugan AN, Welch DP, Weegman BP, Suszynski TM, Papas KK: Commercially available gas-permeable cell culture bags may not prevent anoxia in cultured or shipped islets. Transplantation proceedings 2008, 40:395-400. [47] Hara H, Lin YJ, Zhu X, Tai HC, Ezzelarab M, Balamurugan AN, Bottino R, Houser SL, Cooper DK: Safe induction of diabetes by high-dose streptozotocin in pigs. Pancreas 2008, 36:31-8. [48] Casu A, Bottino R, Balamurugan AN, Hara H, van der Windt DJ, Campanile N, Smetanka C, Cooper DK, Trucco M: Metabolic aspects of pig-to-monkey (Macaca fascicularis) islet transplantation: implications for translation into clinical practice. Diabetologia 2008, 51:120-9. 2009 [49] Anazawa T, Balamurugan AN, Bellin M, Zhang HJ, Matsumoto S, Yonekawa Y, Tanaka T, Loganathan G, Papas KK, Beilman GJ, Hering BJ, Sutherland DE: Human islet isolation for autologous transplantation: comparison of yield and function using SERVA/Nordmark versus Roche enzymes. American Journal of Transplantation 2009, 9:2383-91. (Corresponding Author). [50] Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S: Induction of antioxidant enzymes by curcumin and its analogues in human islets: implications in transplantation. Pancreas 2009, 38:454-60. 2010 [51] Loganathan G, Dawra RK, Pugazhenthi S, Wiseman AC, Sanders MA, Saluja AK, Sutherland DE, Hering BJ, Balamurugan AN: Culture of impure human islet fractions in the presence of alpha-1 antitrypsin prevents insulin cleavage and improves islet recovery. Transplantation proceedings 2010, 42:2055-7. (Corresponding Author). [52] Anazawa T, Balamurugan AN, Matsumoto S, Lafreniere SA, O'Brien TD, Sutherland DE, Hering BJ: Rapid quantitative assessment of the pig pancreas biopsy predicts islet yield. Transplantation proceedings 2010, 42:2036-9. (Corresponding Author). [53] Anazawa T, Balamurugan AN, Papas KK, Avgoustiniatos ES, Ferrer J, Matsumoto S, Sutherland DE, Hering BJ: Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome. Transplantation proceedings 2010, 42:2032-5. [54] Scott WE, 3rd, Weegman BP, Ferrer-Fabrega J, Stein SA, Anazawa T, Kirchner VA, Rizzari MD, Stone J, Matsumoto S, Hammer BE, Balamurugan AN, Kidder LS, Suszynski TM, Avgoustiniatos ES, Stone SG, Tempelman LA, Sutherland DE, Hering BJ, Papas KK: Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance. Transplantation proceedings 2010, 42:2011-5. [55] Balamurugan AN, Breite AG, Anazawa T, Loganathan G, Wilhelm JJ, Papas KK, Dwulet FE, McCarthy RC, Hering BJ: Successful human islet isolation and transplantation indicating the importance of class 1 collagenase and collagen degradation activity assay. Transplantation 2010, 89:954-61.


[56] Bellin MD, Blondet JJ, Beilman GJ, Dunn TB, Balamurugan AN, Thomas W, Sutherland DE, Moran A: Predicting islet yield in pediatric patients undergoing pancreatectomy and autoislet transplantation for chronic pancreatitis. Pediatric diabetes 2010, 11:227-34.

2011 [57] Bellin MD, Freeman ML, Schwarzenberg SJ, Dunn TB, Beilman GJ, Vickers SM, Chinnakotla S, Balamurugan AN, Hering BJ, Radosevich DM, Moran A, Sutherland DE: Quality of life improves for pediatric patients after total pancreatectomy and islet autotransplant for chronic pancreatitis. Clinical gastroenterology and hepatology 2011, 9:793-9. [58] Bellin MD, Sutherland DE, Beilman GJ, Hong-McAtee I, Balamurugan AN, Hering BJ, Moran A: Similar islet function in islet allotransplant and autotransplant recipients, despite lower islet mass in autotransplants. Transplantation 2011, 91:367-72. [59] Anazawa T, Matsumoto S, Yonekawa Y, Loganathan G, Wilhelm JJ, Soltani SM, Papas KK, Sutherland DE, Hering BJ, Balamurugan AN: Prediction of pancreatic tissue densities by an analytical test gradient system before purification maximizes human islet recovery for islet autotransplantation/allotransplantation. Transplantation 2011, 91:508-14. (Corresponding Author). [60] Loganathan G, Dawra RK, Pugazhenthi S, Guo Z, Soltani SM, Wiseman A, Sanders MA, Papas KK, Velayutham K, Saluja AK, Sutherland DE, Hering BJ, Balamurugan AN: Insulin degradation by acinar cell proteases creates a dysfunctional environment for human islets before/after transplantation: benefits of alpha-1 antitrypsin treatment. Transplantation 2011, 92:1222-30. (Corresponding Author). [61] Abouaish J, Graham M, Bansal-Pakala P, Loganathan G, Soltani SM, Tiwari M, Yuasa T, Papas KK, Sutherland DE, McCarthy RC, Hering BJ, Balamurugan AN: Successful isolation and transplantation of nonhuman primate islets using a novel purified enzyme blend. Transplantation 2011, 92:e40-2. (Corresponding Author). 2012 [62] Avgoustiniatos ES, Scott WE, 3rd, Suszynski TM, Schuurman HJ, Nelson RA, Rozak PR, Mueller KR, Balamurugan AN, Ansite JD, Fraga DW, Friberg AS, Wildey GM, Tanaka T, Lyons CA, Sutherland DE, Hering BJ, Papas KK: Supplements in human islet culture: human serum albumin is inferior to fetal bovine serum. Cell transplantation 2012, 21:2805-14. [63] Bellin MD, Balamurugan AN, Pruett TL, Sutherland DE: No islets left behind: islet autotransplantation for surgery-induced diabetes. Current diabetes reports 2012, 12:580-6. [64] Bellin MD, Barton FB, Heitman A, Harmon JV, Kandaswamy R, Balamurugan AN, Sutherland DE, Alejandro R, Hering BJ: Potent induction immunotherapy promotes long-term insulin independence after islet transplantation in type 1 diabetes. American Journal of Transplantation 2012, 12:1576-83. [65] Sutherland DE, Radosevich DM, Bellin MD, Hering BJ, Beilman GJ, Dunn TB, Chinnakotla S, Vickers SM, Bland B, Balamurugan AN, Freeman ML, Pruett TL: Total pancreatectomy and islet autotransplantation for chronic pancreatitis. Journal of the American College of Surgeons 2012, 214:409-24; discussion 24-6. [66] Balamurugan AN, Loganathan G, Bellin MD, Wilhelm JJ, Harmon J, Anazawa T, Soltani SM, Radosevich DM, Yuasa T, Tiwari M, Papas KK, McCarthy R, Sutherland DE, Hering BJ: A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products. Transplantation 2012, 93:693-702. (Corresponding Author).


[67] Velmurugan K, Balamurugan AN, Loganathan G, Ahmad A, Hering BJ, Pugazhenthi S: Antiapoptotic actions of exendin-4 against hypoxia and cytokines are augmented by CREB. Endocrinology 2012, 153:1116-28. [68] Balamurugan AN, Kumaravel V, Pugazhenthi S, Naziruddin B: Islet cell biology, regeneration, and transplantation. International journal of endocrinology 2012, 2012:139787. (Corresponding Author). [69] Bertera S, Balamurugan AN, Bottino R, He J, Trucco M: Increased yield and improved transplantation outcome of mouse islets with bovine serum albumin. Journal of transplantation 2012, 2012:856386.

2013 [70] Wilhelm JJ, Bellin MD, Dunn TB, Balamurugan AN, Pruett TL, Radosevich DM, Chinnakotla S, Schwarzenberg SJ, Freeman ML, Hering BJ, Sutherland DE, Beilman GJ: Proposed thresholds for pancreatic tissue volume for safe intraportal islet autotransplantation after total pancreatectomy. American Journal of Transplantation 2013, 13:3183-91. [71] Lundberg R, Beilman GJ, Dunn TB, Pruett TL, Chinnakotla SC, Radosevich DM, Robertson RP, Ptacek P, Balamurugan AN, Wilhelm JJ, Hering BJ, Sutherland DE, Moran A, Bellin MD: Metabolic assessment prior to total pancreatectomy and islet autotransplant: utility, limitations and potential. American Journal of Transplantation 2013, 13:2664-71. [72] Loganathan G, Graham ML, Radosevich DM, Soltani SM, Tiwari M, Anazawa T, Papas KK, Sutherland DE, Hering BJ, Balamurugan AN: Factors affecting transplant outcomes in diabetic nude mice receiving human, porcine, and nonhuman primate islets: analysis of 335 transplantations. Transplantation 2013, 95:1439-47. (Corresponding Author). [73] Balamurugan AN, Pruett TL: Trying to prevent the clogged drain: optimizing the yield and function of portal vein-infused islets. Digestive diseases and sciences 2013, 58:1170-2. (Corresponding Author). [74] Mueller KR, Balamurugan AN, Cline GW, Pongratz RL, Hooper RL, Weegman BP, Kitzmann JP, Taylor MJ, Graham ML, Schuurman HJ, Papas KK: Differences in glucose-stimulated insulin secretion in vitro of islets from human, nonhuman primate, and porcine origin. Xenotransplantation 2013, 20:75-81. [75] Bellin MD, Beilman GJ, Dunn TB, Pruett TL, Chinnakotla S, Wilhelm JJ, Ngo A, Radosevich DM, Freeman ML, Schwarzenberg SJ, Balamurugan AN, Hering BJ, Sutherland DE: Islet autotransplantation to preserve beta cell mass in selected patients with chronic pancreatitis and diabetes mellitus undergoing total pancreatectomy. Pancreas 2013, 42:317-21. [76] Soltani SM, O'Brien TD, Loganathan G, Bellin MD, Anazawa T, Tiwari M, Papas KK, Vickers SM, Kumaravel V, Hering BJ, Sutherland DE, Balamurugan AN: Severely fibrotic pancreases from young patients with chronic pancreatitis: evidence for a ductal origin of islet neogenesis. Acta diabetologica 2013, 50:807-14. (Corresponding Author)

2014 [77] Balamurugan AN, Naziruddin B, Lockridge A, Tiwari M, Loganathan G, Takita M, Matsumoto S, Papas K, Trieger M, Rainis H, Kin T, Kay TW, Wease S, Messinger S, Ricordi C, Alejandro R, Markmann J, Kerr-Conti J, Rickels MR, Liu C, Zhang X, Witkowski P, Posselt A, Maffi P, Secchi A, Berney T, O’Connell PJ, Hering BJ and Barton FB: Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010. American Journal of Transplantation 2014 Nov;14(11):2595-606. (Corresponding Author). [78] Kitzmann JP, Karatzas T, Mueller KR, Avgoustiniatos ES, Gruessner AC, Balamurugan AN, Bellin MD, Hering BJ, Papas KK: Islet preparation purity is overestimated, and less pure fractions have lower post-culture viability before clinical allotransplantation. Transplantation proceedings 2014, 46:1953-5.


[79] Loganathan G, Graham ML, Spizzo T, Tiwari M, Lockridge AD, Soltani S, Wilhelm JJ, Balamurugan AN, Hering BJ: Pretreatment of donor pigs with a diet rich in soybean oil increases the yield of isolated islets. Transplantation proceedings 2014, 46:1945-9. (Corresponding Author). [80] Bellin MD, Parazzoli S, Oseid E, Bogachus LD, Schuetz C, Patti ME, Dunn T, Pruett T, Balamurugan AN, Hering B, Beilman G, Sutherland DE, Robertson RP: Defective glucagon secretion during hypoglycemia after intrahepatic but not nonhepatic islet autotransplantation. American Journal of Transplantation 2014, 14:1880-6. [81] Scott WE, 3rd, Weegman BP, Balamurugan AN, Ferrer-Fabrega J, Anazawa T, Karatzas T, Jie T, Hammer BE, Matsumoto S, Avgoustiniatos ES, Maynard KS, Sutherland DE, Hering BJ, Papas KK: Magnetic resonance imaging: a tool to monitor and optimize enzyme distribution during porcine pancreas distention for islet isolation. Xenotransplantation 2014, 21:473-9. [82] Kumaravel V, Shriraam V, Anitharani M, Mahadevan S, Balamurugan AN, Sathiyasekaran BW: Are the current Indian growth charts really representative? Analysis of anthropometric assessment of school children in a South Indian district. Indian journal of endocrinology and metabolism 2014, 18:56-62. [83] Chinnakotla S, Radosevich DM, Dunn TB, Bellin MD, Freeman ML, Schwarzenberg SJ, Balamurugan AN, Wilhelm J, Bland B, Vickers SM, Beilman GJ, Sutherland DE, Pruett TL: Long-term outcomes of total pancreatectomy and islet auto transplantation for hereditary/genetic pancreatitis. Journal of the American College of Surgeons 2014, 218:530-43. [84] Suszynski TM, Wilhelm JJ, Radosevich DM, Balamurugan AN, Sutherland DE, Beilman GJ, Dunn TB, Chinnakotla S, Pruett TL, Vickers SM, Hering BJ, Papas KK, Bellin MD: Islet size index as a predictor of outcomes in clinical islet autotransplantation. Transplantation 2014, 97:1286-91. [85] Chinnakotla S, Bellin MD, Schwarzenberg SJ, Radosevich DM, Cook M, Dunn TB, Beilman GJ, Freeman ML, Balamurugan AN, Wilhelm J, Bland B, Jimenez-Vega JM, Hering BJ, Vickers SM, Pruett TL, Sutherland DE: Total pancreatectomy and islet autotransplantation in children for chronic pancreatitis: indication, surgical techniques, postoperative management, and long-term outcomes. Annals of surgery 2014, 260:56-64.

2015 [86] Balamurugan AN , ML Green, AG Breite, G Loganathan,, JJ Wilhelm, B Tweed, L Vargova, A Lockridge, Hughes M, SK Williams, BJ Hering, FE Dwulet, RC McCarthy. Identifying effective enzyme activity targets for recombinant class I and class II collagenase for successful human islet isolation. Transplantation Direct (Published online 23 December 2015). (Corresponding Author). [87] Srinivasan P, Thrower EC, Loganathan G, Balamurugan AN, Subramanian VS, Gorelick FS, and Said HM. Chronic nicotine exposure in vivo and in vitro inhibits vitamin B1 (thiamin) uptake by pancreatic acinar cells. PLoSOne 2015 Dec 3;10(12):e0143575. [88] Colling KP, Blondet JJ, Balamurugan AN, Wilhelm JJ, Dunn T, Pruett TL, Sutherland DE, Chinnakotla S, Bellin M, Beilman GJ. Positive sterility cultures of transplant solutions during pancreatic islet autotransplantation are associated infrequently with clinical infection. Surg Infect (Larchmt). 2015 Apr;16(2):115-23. [89] Papas KK, Bellin MD, Sutherland DE, Suszynski TM, Kitzmann JP, Avgoustiniatos ES, Gruessner AC, Mueller KR, Beilman GJ, Balamurugan AN, Loganathan G, Colton CK, Koulmanda M, Weir GC, Wilhelm JJ, Qian D, Niland JC, Hering BJ. Islet Oxygen Consumption Rate (OCR) Dose Predicts Insulin Independence in Clinical Islet Autotransplantation. PLoS One. 2015 Aug 10;10(8):e0134428.


[90] Robertson RP, Bogachus LD, Oseid E, Parazzoli S, Patti ME, Rickels MR, Schuetz C, Dunn T, Pruett T, Balamurugan AN, Sutherland DE, Beilman G, Bellin MD: Assessment of beta-cell mass and alpha- and beta-cell survival and function by arginine stimulation in human autologous islet recipients. Diabetes 2015 Feb;64(2):565-72.

2016 [91] Camillo Ricordi, Julia Goldstein, Balamurugan AN, multi-authors. NIH-sponsored Clinical Islet Transplantation

Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing Facilities. Diabetes (in press).

[92] Wang LJ, Kin T, O'Gorman D, Shapiro AM, Naziruddin B, Takita M, Levy MF, Posselt AM, Szot GL, Savari O, Barbaro B, McGarrigle J, Yeh CC, Oberholzer J, Lei J, Chen T, Lian M, Markmann JF, Alvarez A, Linetsky E, Ricordi C, Balamurugan AN, Loganathan G, Wilhelm JJ, Hering BJ, Bottino R, Trucco M, Liu C, Min Z, Li Y, Naji A, Fernandez LA, Ziemelis M, Danobeitia JS, Millis JM, Witkowski P. A: Multicenter Study: North American Islet Donor Score in Donor Pancreas Selection for Human Islet Isolation for Transplantation. Cell Transplantion. 2016 Feb 26. Articles in a Book, Pamphlet or Bulletin:

Bottino R., Trucco M., Balamurugan AN, Starzl T.E. (2002). Pancreas and Islet Cell Transplantation. In Best Practice Research Clinical Gastroenterology 2002;16(3):457-74 Bottino R., Roop P., Balamurugan AN, Trucco M. (2005). Alternative, Non-human, Islet Sources. In Insulin Free Times Magazine (IFT), e-magazine. Balamurugan AN, Gopalakrishnan Loganathan, Amber Lockridge, Sajjad M. Soltani, Joshua J. Wilhelm, Gregory J. Beilman, Bernhard J. Hering, and David E. R. Sutherland (2015) Islet Isolation from Pancreatitis Pancreas for Islet Autotransplantation: Chapter 42: M.S. Islam (ed.), Islets of Langerhans (Springer Science Business Media Dordrecht 2015).

Balamurugan AN, Gopalakrishnan Loganathan, Benjamin Tweed, Michael G. Hughes, and Stuart K. Williams. Islet Cell Transplantation. In Organ and Tissue Transplantation. Contemporary Pancreas Transplantation, edited by James W Lim. (in press)

Book, Pamphlet or Bulletin:

Sutherland D.E., Melena B., Blondet J.J., Beilman G.J., Dunn T., Chinnakotla S., Pruett T.L., Freeman M., Balamurgan AN, Bland B., Radosevich D., Hering B.J. (2013) .The Role of Total Pancreatectomy and Islet Autotransplantation for Chronic Pancreatitis. In Chronic Pancreatitis.

CONFERENCES/SEMINARS/SYMPOSIUMS/WORKSHOPS PARTICIPATED (1) XI Annual conference of Indian Association of Biomedical Scientists held in Bombay, India; December 1990. (2) The Seminar on "Recent Trends in Developmental Biology" in the Department of Anatomy, Dr.A.L.M.PG.IBMS, held at the University of Madras in Madras, India; March 1991. (3) XII Annual Conference of Indian Association of Biomedical Scientist held at Madras Medical College, Madras, India; October 1991. (4) XXXVII Annual National Conference of The Association of Physiologists and Pharmacologists of India held at Bangalore; December 1991.


(5) Workshop on "Common Laboratory Equipments, Their Theory, Practice and Maintenance" in the Department of Physiology, DR.A.L.M.PG.IBMS, University of Madras, Madras; March 1992. (6) Satellite Symposium on Sleep and Wakefulness" held at Madras Medical college, Madras; September 1992. (7) XIII Annual Conferences of Indian Association of Biomedical scientists, held in Trivandrum; September 1992. (8) Workshop on "Recent Trends in Teaching of Physiology and Pharmacology" organized by the Vellore chapter of Association of Physiologists and Pharmacologists of India held at Christian Medical College, Vellore; January 1993. (9) XIII Annual Conference of Indian Academy of Neurosciences held at Christian Medical College, Vellore; February 1995. (10) "ROSE 95" Tamil Nadu chapter of the Association of Physiologists and Pharmacologists of India held at Madras Medical College, Madras; October 1995. (11) XVII Annual Conference of Indian Association of Biomedical Scientists held at JIPMER, Pondicherry; October 1996.

(12) International Workshop cum Seminar on Ethics in Science & Medicine held at VMKV Medical College, Salem; January 1998. (13) XIX International Congress of The Transplantation Society held in Miami, FL; August 25-30, 2002. (14) Human Islet Isolation and Transplantation Techniques 2nd Training Workshop held at Banff, Alberta, Canada; September 9-11, 2002, organized by Clinical Islet Program, University of Alberta, Edmonton. (15) 9th International Congress of the “International Pancreas and Islet Transplant Association” held at Royal College of Surgeons, Dublin, Ireland; July 9-11, 2003. (16) 3rd Annual Symposium of “The Clinical and Basic Science of Islet Transplantation” held at Palm Springs, CA, USA; December 4-6, 2003. (17) The Arthur M. Sackler Colloquia of the National Academy of Sciences “Therapeutic Vaccines - Realities of Today and Hopes for Tomorrow” held at National Academy of Sciences, Washington, D.C.; April 1-3, 2004. (18) Golden APPICON- 50th Annual National Conference of The Association of Physiologists and Pharmacologists of India held at Bangalore; December 2004. (19) World Transplant Congress held in Boston, MA; July 22-27, 2006. (20) Cell Transplantation Society-International Pancreas Islet Transplantation Association-International Xenotransplantation Assocation-2007 Joint Conference held in Minneapolis, MN; September 15-20, 2007.

(21) National Islet Cell Resource Center’s 3rd annual Islet Workshop held at the University of Illinois at Chicago, October 18, 2007.

(22) National Islet Cell Resource Center’s 4th annual Islet Workshop held in Newport Beach, CA; October 3, 2008.

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