Bacterial Growing1

8/9/2019 Bacterial Growing1

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Nui cy vi sinh vt

Lecture 3


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Pht trin vi sinh vt linquan ti tng s t bo , khng lin quan ti ln ca t bo

Cc i hi cho s pht trinVt l

Ho hc


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Cc i hi vt lNhit

-a lnh (psychrophiles) (cold lovingmicrobes )

Di nhit 0oC - 20 oC -a m (mesophiles) (moderate temp.

loving microbes)Di nhit 20oC - 40 oC

Chi nhi t (thermophiles) (heat lovingmicrobes)

Di nhit 40oC - 100 oC


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pHHu ht vi sinh vt pht trin trong pH6.5 - pH 7.5

Rt t c th pht trin d-i pH 4.0 Nhiu thc phm, nh- sauerkraut, d-a mui, v

pho mt -c bo qun khi s h- hng do acid -c to ra trong qu trnh ln men


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p sut thm thuHu ht vi sinh vt nhn -c tt c

cc cht dinh d-ng trong dung dch tmi tr-ng n-c xung quanhTnh c-ng (Tonicity)

Tnh c-ng va (isotonic)

Tnh c-ng nhiu (hypertonic) Tnh c-ng t (hypotonic)


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Cells


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Cc i hi ho hc Macro & M icro Eleme nts


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Oxyg enVi sinh vt cn phi -c phn loi

da trn cc i hi oxygen


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1. Vi sinh vt hiu kh (obligate Aerobe)


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2. Vi sinh vt k kh (obligate Anaerobe)


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3. Vi sinh vt hiu kh khng btbuc & k kh khng bt buc(Facultative Aerobe &Facultative

Anaerobes)


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4. Vi sinh vt cn rt t kh(Microaerophilic)


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Oxy gen lm cht mt s vi sinh v

Tt c vi sinh vt sinh ra superoxide ( O2-)

Superoxide c cho t bo (ly i cc in t)

Superoxide cn phi -c trung ho


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Superoxide dismutase

O2- + O2- + 2 H+ -------> H2O2 + O2

Hydrogen peroxide cng l c t cho tbo v cn phi trung ho n


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C atalase

2 H2O2 --------> 2 H2O + O2

Vi sinh vt hiu kh bt buc khuyt:Superoxide dismutase ( SOD )

Catalase


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C c k thut nui c y c bit

1. Vi khun k kh (AnaerobicBacteria)

a. Gim bt mi tr-ng (Reducing Media)

b. Bung k kh (AnaerobicContainer)

c. Agar Stab

d. Agar Shake (AGAR OVERLAY TECHNIQUE)


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C c k thut nui c y c bit 2. Vi khun t hiu kh

(Microaerophilic Bacteria)Pht trin tt nht O2 gim bt v tng

mc

Khng kh bnh th-ng 21 % O20 .3 n 0.03 %

CO2


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Vi khun t k kh

(Microaerophilic Bacteria) A. Bnh nn (Candle Jar)

16 % O2

4 % C O2


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Agar Stab


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M i tr-ng chn lc c ch s ph t trin ca mt s vi khuntrong khi li chn lc s pht trin chocc vi khun khcV d:

Brilliant Green Agar Mu c ch s pht trin vi khun Gram (+)Chn lc cho vi khun Gram (-)Hu ht c c nhim khun -ng rut gy ra do

vi khun Gram (-)


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M i tr-ng chn lc EMB (Eosin Methylene Blue)

Mu c ch vi khun Gram (+)

Chn lc cho vi khun Gram (-)

Hu ht c c nhim khun -ng rutgy ra do vi khun Gram (-)


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M i tr-ng khc bit

S khc bit gia c c sinh vt kh c nhaupht trin trn cng mt a

V d:a thch mu (TSA c 5% m u cu)

S dng s khc nhau phn bit cc kiuS treptococci


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A lpha Hemo lytic Streptococci

Inco mple te lysis of R B Cs


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B eta Hemo lytic Streptococci

Co mple te lysis of R B Cs


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Ga mm a Hemo lytic Streptococci

No lysis of R B Cs


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M i tr-ng chn lc v khc bit

Mannitol Salt Agar S dng phn bitS taphy lococcus aureus

Mannitol Salt Agar Nng mui cao (7.5%) c ch hu ht c c

vi khun

-ng MannitolCh th pH (Chuyn mu vng khi acid)


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M i tr-ng chn lc v khc bit MacConkey s Agar

S dng phn bitS almonella

MacConkey s Agar Bile salts v crystal violet (c ch vi

khun Gram (+))lactose

Ch th pHMa ny Gra m (-) enter ic no n- pa tho genic bac ter ia ca n f er mentlac to se, Salmonella ca n n o t


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S sinh sn nhn i - uncheckeE. coli thi gian ti to l 20 pht.20 th h (khong 7 gi.)

1 triu t bo

30 th h (khong 10 gi.)1 t t bo

72 th h (khong 24 gi.)1 x 1021

1,000,000,000,000,000,000,000 t bo


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C c y u t hn ch trong mi tr-n

Thiu thc n, n-c hoc chtdinh d-ng

Khng giantch t c c cht thi trao i

cht

Thiu oxygenThay i pH

Nhit


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C c pha pht trin

1. Pha Lag

2. Pha Log

3. Pha Stationary

4. Pha Death


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1. Pha Lag Vi khun u tin -c -a vo mi

tr-ng ni cyVi khun checking out mi tr-ng

xung quanhCc t bo hot ng trao i cht

rt tch ccS l-ng t bo thay i rt t1 gi n vi ngy


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2 . Pha Log

T bo ph t trin nhanh (exponentialgrowth)

Qun th tng gp i sau mi th hVi sinh vt rt nhy cm vi cc iu kin

bt liantibioticsanti-microbial agents


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4 .Pha Death

T l cht > t l ti to

Do c c yu t hn ch trong mi tr-ng


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m vi khun

c mi tr-ng - 10 triu t bo vi khuntrn ml

Pha long hng lot (Serial Dilution)

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