AVIAN TUMOR

Prepared By

DEWI RATIH AGUNGPRIYONO, DVM, PhD

Division of PathologyDepartment of Veterinary Clinic, Reproduction and Pathologyp y , p gyFaculty of Veterinary Medicine, Bogor Agricultural University

D Diseases causing avian tumor :

1. Marek (MD)( )

2 Avian Leukosis (AL)2. Avian Leukosis (AL)

3 Reticul end theli sis (RE)3. Reticuloendotheliosis (RE)

ETIOLOGYMDV ALV REVMDV ALV REV

α Herpes virus Retrovirusα Retrovirusp RetrovirusSerotype 1 oncogenicSerotype 2 non-

oncogenic A i L k i Vi

Avian Sarcoma Virus

Sub grup A

oncogenicSerotype 3 HVT non-

oncogenic

Avian Leukosis Virus

Sub grup Boncogenic

Sub grup J

DNA Virus RNA Virus RNA VirusDNA Virus RNA Virus RNA Virus

TYPE OF TUMOR CELLSMD ALV REV

Lymphoma LymphomaSarcoma Lymphoma (mononuclear pleiomorphic cells :

LymphomaTumor of blood vessel, tumor of loose connective tissue

uniform lymphoblast p p

lymphocytes, lymphoblast, plasma

connective tissue, epithelial tumor

tumor cells, immature and mature

cell, macrophage) Lymphoma and Leukosis

uniform lymphoblast tumor cells, erythroblast erythroblast, myeloblast, myelocyte

TYPE OF TUMOR CELLSMD ALV REV

TRANSMISSIONMD ALV REV

Horizontal Horizontal, Vertical

Horizontal, Vertical Direct Contact Direct Contact

Indirect : Inhalation

Gross Pathology MD ALV REV

Nerve : enlarged Nerve : enlargedg

Skin : feather follicle Viscera : visceral tumor Viscera : visceral

tumor Viscera : visceral tumorSkin : feather follicle

tumorEye : greyy g y

8 – 10 weeks > 24 weeks > 24 weeks

Not all tumor cases could be observed grossly !

Gross Pathology gy

Grey Eye (right)lymphoid infiltration of iris that causes white discoloration. The pupil is often irregular (as shown here) and does not respond to ) pchanges of light. A normal eye is on the left.

Visceral Tumor (liver)Visceral Tumor (liver)

Gross Pathology

b /111/02patoipb U/111/02

Visceral Tumor(liver and spleen)( p )

Gross Pathology

Visceral Tumor (spleen)

patoipb U/30/01

Thickened proventricular wallGross Pathology

T i l (h ti)Tumor viseral (hati)patoipb U/60/01

Gross Pathology Gross Pathology

patoipb U/30/01

Visceral Tumor (lung)

Gross Pathology

Visceral Tumor (kidney)

Gross Pathology gy

Nerve Tumor (nervus Brachialis)

Gross Pathology

Nerve Tumor (nervus Ischiadicus)

Gross Pathology

The dorsal root ganglia from bird with Marek's disease

Gross Pathology

Skin Tumor / Feather Follicle Tumor

Gross Pathology gy

Skin Tumor / Feather Follicle Tumor

Gross Pathology gy

large subcutaneous lymphomas may be observed throughout the body (shown here in the leg on the left). A normal leg is shown on the right for comparison

Gross Pathology

os sternum tumor

Gross Pathology Gross Pathology

Costae Tumor

G P th l Gross Pathology

B F b i i TBursa Fabricious Tumor

Gross Pathology

Liver Hemangioma

Microscopic Lesion MD ALV REV

Nerve : infiltration Nerve : infiltration of pleiomorph lymphocyte, demyelinization,

mature and immature lymphoblast

Schwann cells proliferationB i i l Brain : perivascular accumulation lymphocyte pleiomorph lymphocyte pleiomorph, spread to neuropil, necrotizing malaciag

Histopathology p gy

N m lNormal

patoipb S/30/02Nerve Tumor

Histopathology p gy

Demyelinization

Histopatology p gy

tumor cells perivascular cuffing

Histopathology p gy

patoipb S/30/02Malacia

Histopathology MD ALV REV

Viscera : interstitial i filt ti f l i h

Visceral : interstitial i filt ti f

Visceral : interstitial i filt ti f infiltration of pleiomorph

lymphocytes tumor cellsinfiltration of lymphoblast tumor cells in many visceral

infiltration of lymphoblast tumor cells in many visceral

Skin : accumulation of pleiomorph lymphocyte

cells in many visceral organ

cells in many visceralorgan

pleiomorph lymphocyte at feather follicle epitheliumBone Marrow : multifocal accumulation l h t l i h

Bone Marrow : diffuse accumulation limfoblast tumor cellslymphocyte pleiomorph limfoblast tumor cells

Histopathology

Liver Tumor

Histopathology

interstitial ovary tumor cells

Histopathology

Feather Follicle Tumor

Histopathology MD ALV REV

Lymphoreticular : Lymphoreticular : lymphoid cells cytolisis → follicle depletion; Bursal interfollikular

Proliferation of lymphoblast tumor cells at spleen (red Bursal interfollikular

pleiomorph lymphocyte tumor cells infiltration

cells at spleen (red pulp) and bursa (intrafollicullar); tumor cells infiltration (intrafollicullar);

Vascular : Atherosclerosis

Vascular : Circulation of proliferated tumor cells (leukosis)cells (leukosis)

Histopathology

Interfollicular Bursa Fabricious Tumor, Lymphoid Follicle Atrophy

Histopathology

Interfollicular Bursa Fabricious Tumor

Intrafollicular Bursa Fabricious Tumor

Histopathology MD ALV REV

antigen Detection of antigen Detection of antigen Detection of gsurface

MATSA 5-40% MATSA negatif MATSA negatif

gsurface

gsurface

Ig M < 5 %Sel B 3-25%Sel T 60-90%

MATSA negatif Ig M 91-99 %Sel B 91-99%Sel T rare

MATSA negatif Ig M ?Sel B rareSel T plentySel T 60 90% Sel T rare Sel T plenty

MATSA : Marek’s Disease Associated Tumor Surface Antigen

Clinical Sign

MD ALV REVTransient Paralysis Sub clinic Sub clinicy

(Late clinical onset) (Late clinical onset)

patoipb U/116/02

ALV Infected Male

PreventionMD ALV REV

Vaccination Biosecurity BiosecurityBiosecurity

yBreeder control

y

(Culling positive parents)Breeder control

(Culling positive parents)

Non-specific Lesion

Egg Yolk Peritonitis

Non-Spesific Lesion

interstitial ovary tumor cells

Non-spesific Lesion

patoipb U/111/02

Necrotic Enteritis

patoipb U/111/02

Necrotic Enteritis

Non Spesific Lesion

T ll ithi i t ti l l i iTumor cells within intestinal lamina propria