Automation in Blood Processing and Improvement in Quality of the
Automation-Ready Purification Chemistries · 2020-03-13 · In-Lab Automation . Support Available ....
Transcript of Automation-Ready Purification Chemistries · 2020-03-13 · In-Lab Automation . Support Available ....
SV 96 Vacuum-Based Chemistries
ReliaPrep™, MagneSil®, simplyRNA & MagneHis™ Paramagnetic Particle-based Chemistries
Plasmid DNA Wizard® MagneSil™ Plasmid Purification System Wizard MagneSil Tfx™ System Wizard® SV 96 Plasmid DNA Purification System
Genomic DNA ReliaPrep™ 96 gDNA Miniprep HT System ReliaPrep™ Large Volume HT gDNA Isolation System Wizard® SV 96 Genomic DNA Purification System Wizard® Magnetic 96 DNA Plant System MagneSil® Blood Genomic, Max Yield System
Total RNA
ReliaPrep™ simplyRNA HT System, Custom MagneSil® Total RNA mini-Isolation System
DNA Fragments
Wizard® SV 96 PCR Clean-Up System
Recombinant Protein
MagneHis™ Protein Purification System
Automation-Ready Purification Chemistries
Product Cat. # Size
Wizard® MagneSil® Plasmid Purification System
A1630 4 x 96 preps
A1631 8 x 96 preps
Wizard® MagneSil® Plasmid Purification System, HTP1
A1635 100 x 96 preps
Each catalog item contains sufficient material for indicated number of preps: • Cell Resuspension Solution • Cell Lysis Solution
•Neutralization Solution •MagneSil® BLUE •MagneSil® RED
• Elution Buffer • Collection Plates
MagneBot, MagneSil and Wizard are trademarks of Promega Corporation. Products may be covered by existing or
pending patents. Please visit www.promega.com for more information.
Wizard® MagneSil® Plasmid Purification System Provides a simple and reliable method for rapid isolation of plasmid DNA using silica-coated paramagnetic particles in a 96-well, high-throughput format without centrifugation or vacuum manifolds.
• Automation Friendly: Methods available for liquid handlers including Beckman Coulter and Tecan. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full plate in ~1 hour (96-well head) to ~1½ hours (8-channel).
Isolated plasmid DNA ready for use:
•Sequencing •PCR •Transformation
Custom & Bulk
Available
In-Lab Automation
Support Available
Plasmid DNA
96-well
Produce Cleared Lysate with
MagneSil® Blue
Recover Plasmid
Capture Plasmid with
Magnesil® Red
Capture & release washes with 80% ethanol
•Probe Generation •Restriction Digestion •Cloning
Product Cat. # Size
Wizard MagneSil Tfx™ System A2380 4 x 96 preps
Contains sufficient material for indicated number of preps: • Cell Resuspension Solution • Cell Lysis Solution
•Neutralization Solution •MagneSil® BLUE • Endotoxin Removal Resin
•MagneSil® RED • 4/40 Wash Solution •Nuclease-Free Water
MagneBot is a trademark and Wizard MagneSil Tfx is a trademark of Promega Corporation. Products may be covered by
existing or pending patents. Please visit www.promega.com for more information.
Wizard MagneSil Tfx™ System Provides a simple and reliable method for rapid isolation of transfection-quality plasmid DNA using silica-coated paramagnetic particles in a 96-well, high-throughput format without centrifugation or vacuum manifolds.
• Automation Friendly: Methods available for liquid handlers including Beckman Coulter. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full plate in ~1 hour (96-well head) to ~1½ hours (8-channel).
Custom & Bulk
Available
In-Lab Automation
Support Available
Plasmid DNA
96-well
Produce Cleared Lysate with
MagneSil® Blue
Recover Plasmid
Capture Plasmid with
Magnesil® Red
Capture & release washes with 80% ethanol
Treat cleared lysate with Endotoxin Removal Resin
Capture & release wash with 4/40 Wash Solution
Isolated plasmid DNA ready for use:
•Transfection •Sequencing •PCR •Transformation
•In vitro transcription •Probe Generation •Restriction Digestion •Cloning
Wizard® SV 96 Plasmid DNA Purification System
Product Cat. # Size
Wizard® SV 96 Plasmid DNA Purification System
A2255 5 x 96 preps
Wizard® SV 9600 Plasmid DNA Purification System
A2258 100 x 96
preps
Vac-Man® 96 Vacuum Manifold A2291 Each
Provides a simple and reliable method for rapid isolation of plasmid DNA using silica membranes in a 96-well, high-throughput format.
• Automation Friendly: Methods available for liquid handlers that can use vacuum methods including Beckman Coulter, Caliper, Eppendorf, PerkinElmer and Tecan. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full plate in 60 minutes or less
Crude Lysate
filter
cleared lysate
Binding Membrane
waste
Neutralize Wash
Apply Vacuum
Apply Vacuum
Apply Vacuum
Apply Vacuum
Dry Elute
Apply Vacuum
Elution plate
.
.
. . .
.
. . . . . . .
. .
. . .
2X
Lysate Clearing
Plate
Binding Plate
Reagents supplied to make crude lysate
Cat. #A2255 contains all items listed below. Cat. #A2258 contains only the underlined items. •Deep-Well Plate (for culturing bacteria) • Cell Resuspension Solution
• Cell Lysis Solution •Neutralization Solution • Lysate Clearing Plates
• Binding Plates •Wash Solution •Nuclease-Free Water
• Elution Plates • Plate Sealers •Alkaline Protease Solution (optional use)
Wizard and Vac-Man are trademarks of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
Custom & Bulk
Available
In-Lab Automation
Support Available
Plasmid DNA
Manual or
Automated
96-well
Isolated plasmid DNA ready for use: •Sequencing •PCR •Transformation •Probe Generation •Restriction Digestion •Cloning
ReliaPrep™ 96 gDNA Miniprep HT System
Product Cat. # Size
ReliaPrep™ 96 gDNA Miniprep HT System
A2670 1 x 96 preps
A2671 4 x 96 preps
Isolate genomic DNA (gDNA) from whole blood or saliva collected in Oragene®DISCOVER or Oragene®DNA collection devices. The system has been automated on third-party liquid-handlers, allowing walkaway purification of genomic DNA.
• Automation Ready: Methods available for multiple liquid handlers. Learn more at www.promega.com/automethods
• Rapid, Safe Results: Recover gDNA from 96 samples in only 2.5 hours minutes without the use of hazardous chemicals like phenol and chloroform. Ready for a variety of applications including PCR, qPCR, microarrays and next-gen sequencing.
Contains sufficient material for indicated number of preps: • Proteinase K Solution • Cell Lysis Buffer
• ReliaPrep™ Magnetic Resin • Binding Buffer •Wash Buffer
• 25mM Tris-HCl (pH 8.0) • 10mM EDTA (pH 8.0) • RNase A Solution (4mg/ml)
ReliaPrep is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit
www.promega.com for more information. Oragene is a registered trademark of DNA Genotek Inc.
Custom & Bulk
Available
Genomic DNA
Manual or
Automated
In-Lab Automation
Support Available
Capture gDNA with ReliaPrep™ Magnetic
Resin & Binding Buffer
Blood or saliva samples are lysed in Lysis Buffer plus
Proteinase K
Capture and release washes in Wash Buffer and then 50% ethanol
Recover gDNA in 25mM Tris-HCL Buffer
Isolated genomic DNA ready for use: •PCR & qPCR •Microarrays •Next-gen sequencing
96-well Ask about platforms
& configurations
ReliaPrep™ Large Volume HT gDNA Isolation System
Product Cat. # Size
ReliaPrep™ Large Volume HT gDNA Isolation System
A1751 96 x 10ml preps
ReliaPrep™ LV 32 HSM Instrument*
A1715 Each
Tecan Freedom EVO® – HSM Workstation
XAT1000 Each
Isolate genomic DNA (gDNA) from whole blood, regardless of sample storage or shipping conditions. The system has been automated on the Tecan Freedom EVO® Workstation, allowing walkaway purification of genomic DNA.
• Walkaway Automation: Load the instrument with samples, add the reagents, start the protocol, pure gDNA from up to 32 samples in less than 4 hours. No manual intervention.
• Reagent & Time Savings: Each sample can be a different volume between 1 and 10ml. Instrument detects blood volume in each sample and scales reagents accordingly.
Contains sufficient material for indicated number of preps: • Cell Lysis Buffer • Binding Buffer
• ReliaPrep™ Resin •Wash Buffer •Alkaline Protease
• Proteinase K Solution •Nuclease-Free Water
Service agreements available. ReliaPrep is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit www.promega.com for more information. Freedom EVO is a registered trademark of Tecan Group Ltd.
Custom & Bulk
Available
Genomic DNA
Automated
In-Lab Automation
Support Available
1-32 X
1 to 10ml Blood
Blood volume detected
Lysis Binding to Particles
Capture & release washes
Elute pure DNA
Tecan Freedom EVO®– HSM Workstation
Wizard® SV 96 Genomic DNA Purification System
Product Cat. # Size
Wizard® SV 96 Genomic DNA Purification System
A2371 4 x 96 preps
Vac-Man® 96 Vacuum Manifold A2291 Each
Proteinase K V3021 100mg
Provides a simple and reliable method for rapid isolation of genomic DNA from cultured cells or tissue lysates including rodent tail using silica membranes in a 96-well, high-throughput format.
• Automation Friendly: Methods available for liquid handlers that can use vacuum methods including Beckman Coulter, Caliper, Eppendorf, PerkinElmer and Tecan. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full plate in 30 minutes from cells or 45-60 minutes from tissue lysates digested with proteinase K.
Catalog #A2371 contains sufficient material for indicated number of preps: •Wizard® SV Lysis Buffer • Binding Plates
•Wizard® SV Wash Solution •Nuclease-Free Water • 96-well Deep Well Plates
• Tissue Digest Reagents (Nuclei Lysis Solution, RNase A Solution, 0.5M EDTA. Proteinase K available separately.)
Wizard and Vac-Man are trademarks of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
Apply Vacuum
Dry Elute
Apply Vacuum
Elution plate
Transfer
Apply Vacuum
. .
Add Wizard® SV Lysis Buffer.
Mix by pipetting
PBS washed
cell monolayer
Culture plate
Wash
Apply Vacuum
. .
Binding Plate
waste
3X
Custom & Bulk
Available
Genomic DNA
Manual or
Automated
96-well
In-Lab Automation
Support Available
Isolated genomic DNA ready for use: •qPCR •PCR
96-well
Custom & Bulk
Available
Genomic DNA
Manual or
Automated
In-Lab Automation
Support Available
Wizard® Magnetic 96 DNA Plant System
Product Cat. # Size
Wizard® Magnetic 96 DNA Plant System
FF3760 2 x 96 preps
FF3761 4 x 96 preps
Provides a simple and reliable method for rapid isolation of genomic DNA from leaf tissue or seeds using silica-coated paramagnetic particles in a 96-well, high-throughput format . Procedure requires solubilization of sample with a bead beater or grinding apparatus.
• Automation Friendly: Methods available for liquid handlers including Beckman Coulter Biomek® FX. Learn more at www.promega.com/automethods
• Rapid, Safe Results: Recover gDNA in 40 minutes (96-well head) without lengthy incubations or hazardous chemicals like phenol and chloroform. Ready for amplification-based applications.
Contains sufficient material for indicated number of preps: • Lysis Buffer A • Lysis Buffer B
•Wash Buffer, Plant •MagneSil® Paramagnetic Particles • Collection Plate (4-pack) Wizard and MagneSil are registered trademarks of Promega Corporation. Products may be covered by existing or pending
patents. Please visit www.promega.com for more information. Biomek is a registered trademark of Beckman Coulter.
Capture gDNA with MagneSil®/Lysis
Buffer B
Plant leaf tissue or seeds homogenized
in Lysis Buffer A
Capture and release washes in
Wash Buffer, Plant
Recover gDNA
Isolated genomic DNA ready for use: •qPCR •PCR •RAPD
96-well
Custom & Bulk
Available
Genomic DNA
Automated
In-Lab Automation
Support Available
MagneSil® Blood Genomic, Max Yield System
Product Cat. # Size
MagneSil® Blood Genomic, Max Yield System
MD1360 1 x 96 preps
Provides a consistent and simple method for rapid isolation of high purity genomic DNA (gDNA) from 200µl of blood and other sample types. This system is particularly well suited to gDNA isolation from small numbers of cells.
• Automation Ready: Methods available for liquid handlers including Beckman Coulter Biomek® FX. Learn more at www.promega.com/automethods
• Rapid, Safe Results: Recover gDNA in 90 minutes without lengthy incubations or hazardous chemicals like phenol and chloroform. Ready for amplification-based applications.
Contains sufficient material for indicated number of preps: • Lysis Buffer, Blood • Salt Wash, Blood
•MagneSil® Paramagnetic Particles •Alcohol Wash, Blood • Elution Buffer, Blood
•Anti-Foam Reagent MagneSil is a registered trademarks of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
Capture gDNA with MagneSil® PMPs/ Lysis Buffer, Blood
Blood, cells, tissues, or bacterial samples are lysed
in Lysis Buffer, Blood
Capture and release washes in Lysis, Salt & Alcohol Washes, Blood
Recover gDNA in Elution
Buffer, Blood
Isolated genomic DNA ready for use: •PCR •qPCR •Multiplex PCR •SNP genotyping
ReliaPrep™ simplyRNA HT System, Custom
Product Cat. # Size
ReliaPrep™ simplyRNA HT System, Custom
X9601 4 x 96 preps
Provides a method for adapting the Maxwell® 16 simplyRNA chemistry to 96 well plate format on third party liquid handlers. Produces high purity, concentrated total RNA samples ready for a variety of downstream applications.
• Automation Ready: Current method available for the Beckman Coulter Biomek® FX liquid handler. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full 96 well plate in only 75 minutes.
Each catalog item contains sufficient material for indicated number of preps: • RNA Resin ( Paramagnetic Particles, PMPs) • Binding Solution 1
• RNA DNase Wash • Binding Solution 2 •Wash Solution
•Homogenization Solution • Cell Lysis Buffer (CLD) •DNase I
• 1-Thioglycerol •Nuclease-Free Water • Plus other components
Maxwell is a registered trademark of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
Custom & Bulk
Available
Total RNA
Automated
In-Lab Automation
Support Available
Isolated total RNA ready for use: •RT-qPCR •Microarray •RT-PCR •1st Strand cDNA
Recover Total RNA
Capture RNA on simplyRNA PMPs
Capture & release washes
Cells or mammalian/ plant tissue in
Homogenization Buffer and then Lysis Buffer
Resuspend PMPs in DNase I Solution
Capture & release washes
Add DNase Stop Solution
96-well
Product Cat. # Size
MagneSil® Total RNA mini-Isolation System
Z3351 4 plates
Contains sufficient material for indicated number of plates whether 96- or 384-well format: •MagneSil® RNA Paramagnetic Particles • RNA Lysis Buffer
•DNase I (lyophilized) • Yellow Core Buffer (DNase I resuspension) •MnCl2 Solution (DNase I activation)
•DNase Stop Solution •Nuclease-Free Water
MagneSil is a registered trademark of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
MagneSil® Total RNA mini-Isolation System Provides a simple and reliable method for rapid isolation of total RNA from cultured cells, whole blood or tissue lysates using silica-coated paramagnetic particles in a 96-well or 384-well, high-throughput format without centrifugation or vacuum manifolds.
• Automation Friendly: Methods available for liquid handlers including Beckman Coulter. Learn more at www.promega.com/automethods.
• Rapid Results: Process a 96-well plate in 50 minutes (96-well head) to 80 minutes (8-channel). Process a 384-well plate in 90 minutes (96-well head).
96- & 384-well
Recover Total RNA
Capture RNA on MagneSil® RNA PMPs
Capture & release wash with
90% ethanol
Cells, Blood or Tissue homogenates in RNA Lysis Buffer
Resuspend PMPs in DNase I Solution
Isolated Total RNA ready for use:
•RT-qPCR •RT-PCR •1st Strand cDNA
Capture & release washes with 90% ethanol
Add DNase Stop Solution
Total RNA
Custom & Bulk
Available
In-Lab Automation
Support Available
Wizard® SV 96 PCR Clean-Up System
Product Cat. # Size
Wizard® SV 96 PCR Clean-Up System
A9341 4 x 96 preps
A9342 8 x 96 preps
A9345 100 x 96 preps
Vac-Man® 96 Vacuum Manifold
A2291 Each
Provides a simple and reliable method for rapid purification of 100bp to 10kb PCR product away from primers, primer dimers, and reactants using silica membranes in a 96-well, high-throughput format.
• Automation Friendly: Methods available for liquid handlers that can use vacuum methods including Beckman Coulter, Caliper, Eppendorf, PerkinElmer and Tecan. Learn more at www.promega.com/automethods.
• Rapid Results: Process a full plate in 20 minutes with 90% recovery.
Catalog # A9341, A9342 and A9345 contains sufficient material for indicated number of preps:
•Membrane Binding Solution • Binding Plates •Nuclease-Free Water
• Collection Plates
Wizard and Vac-Man are trademarks of Promega Corporation. Products may be covered by existing or pending patents.
Please visit www.promega.com for more information.
Transfer Wash
Apply Vacuum
Apply Vacuum
Apply Vacuum
Dry Elute
Apply Vacuum
Elution plate
. .
. . .
3X Binding
Plate
Add equal volume
Membrane Binding Solution to PCR reaction
PCR Plate . .
waste
Custom & Bulk
Available
DNA Fragments
Manual or
Automated
96-well
In-Lab Automation
Support Available
Isolated PCR DNA ready for use: •Sequencing •Labeling •Restriction Digestion •Cloning •Microarray Analysis
MagneHis™ Protein Purification System
Product Cat. # Size
MagneHis™ Protein Purification System
V8550 325 preps
MagneHis™ Ni-Particles V8565 10ml
The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate in an automated procedure • Automation Friendly: Methods available for liquid handlers like Beckman-Coulter and
Tecan. Learn more at www.promega.com/automethods.
• Rapid Purification & High Recovery: Process a full plate in ~1 hour (96-well head) and MagneHis™ Ni-Particle bind ~1mg protein/ml.
Catalog # V8550 contains sufficient material for indicated number of preps:
•MagneHis™ Ni-Particles •MagneHis™ Binding/Wash
Solution •MagneHis™ Elution Buffer • FastBreak™ Cell Lysis Reagent
•DNase I (lyophilized) MagneHis is a trademark of Promega Corporation. Products may be covered by existing or pending patents. Please visit
www.promega.com for more information.
Custom & Bulk
Available
Recombinant Protein
Manual or
Automated
In-Lab Automation
Support Available
96-well
Capture polyhistidine protein with
MagneHis™ Ni-Particles
Bacterial, Insect, or Mammalian cells lysed with FastBreak™ Lysis
Buffer
Capture & release washes
Elute Recover polyhistidine
protein
www.promega.com/automethods
In-Lab Automation
Support Available
Verified methods for: • Nucleic acid purification • Recombinant protein purification • Cell-based assays
Variety of platforms*: • Aurora Discovery • Beckman Coulter • Caliper Life Sciences • CyBio • Deerac Fluidics • Eppendorf • PerkinElmer • Tecan
* Promega chemistries may not have automethods for every platform listed.
Screen capture of www.promega.com/automethods, November 2011.
Download ready-to-use automated methods for your instrument.
Additional Field Support Services
Training (Cat. # S3001, S3002, S3003) Customized training assists you with implementation of Promega technologies in your laboratory. Training is available online (S3001), in your laboratory (S3002), or at a Promega facility (S3003). Integration Support (Cat. # S3004) Integration Support assists you in combining Promega technologies, such as those outlined here, with laboratory instrumentation, customizing protocols for new sample types, as well as improving laboratory efficiency. Applications Development (Cat. # S3005) Applications Development adapts Promega technologies to meet your new need such as the development of modified protocols that provide a solution for a previously unsupported application.
Scientists at Your Service
You’ll be surprised what we’ll do to help you succeed.
Have questions about automating Promega chemistries on or not on the www.promega.com/automethods list?
More on Integration Support
Are you currently running/investigating Promega chemistries & need further help
optimizing or implementing?
Need a custom automation method developed specific to
your research needs?
Don’t have the time or know-how to implement
Promega chemistries?
“What if…?”
…greater than 10 years of industry experience integrating chemistries and instruments in
high-throughput environments
Kevin Kershner 608-219-5126
Bob Ray 610-427-9338
Field Support Scientists
Custom & Bulk Ordering
Need it Customized?
We can help! • Custom products
• Custom dispensing
• Bulk quantities
• OEM solutions, too
Ask your Promega Representative
or visit
www.promega.com/custom