Austrian Pinot blanc wines: Typicity, wine styles and the influence … · ethyl dodecanoate,...

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BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005 42 nd World Congress of Vine and Wine Austrian Pinot blanc wines: Typicity, wine styles and the influence of different oenological decisions on the volatile profile of wines C. Philipp, S. Sari, P. Eder, E. Patzl-Fischerleitner, and R. Eder ohere Bundeslehranstalt und Bundesamt f ¨ ur Wein- und Obstbau, Wienerstraße 74, 3400 Klosterneuburg, Austria Abstract. Pinot blanc is found in all wine regions of Austria. However, it plays an economically subordinate role compared to the most important white grape variety, Gr¨ uner Veltliner. The concept of wine typicity and terroir is that the geographical origin and oenological practices, along with the grape variety, make an important contribution to the final expression of the finished wine. The purpose of this study was to discuss different styles of Austrian Pinot blanc wines and to discuss the various wine production decisions in terms of wine stylistics and aromatic profile. 131 commercial Austrian wine samples of the Pinot blanc variety of the 2015 to 2017 vintages were collected and the manufacturer’s practices were determined by means of a questionnaire related to the wine styles. Using various GC-SIM-MS methods, more than 100 essential volatile substances were quantified. A trained panel, an expert panel and consumers evaluated the wines based on typicity and quality. 15 typical wines were then assessed on a sensory basis by means of predetermined aroma and wine styles. The four Austrian Pinot blanc wine styles previously discussed and presented by the same authors (citrus (1st style), green apple and green pear (2nd style), ripe yellow apple, quince, cooked pear, bread and honey notes (3rd style), as well as intensive oak notes (4th style)) were able to be partially reproduced by the trained panel. The following three wine styles could be better understood by the consumers: fresh fruity (style A), complex aroma with or without moderate wood (style B) and lots of wood aroma (style C). These styles (A–C) were able to withstand a sensory examination and a discriminant analysis by substance. An oenological guideline could help the producers to achieve the respective style. Austria’s wine-growing area is probably too small and the importance of the Pinot blanc variety for the individual areas still too low to support the terroir concept for this grape variety. 1. Introduction Pinot blanc is an old grape variety with some fans. Interestingly, it is experiencing an unexpected renaissance in Central Europe, especially in Germany, South Tyrol and Austria. In Austria, specialisations are to be found where Pinot blanc has now been approved as DAC (Districtus Austriae Controllatus). Accordingly, the regions around the Leithaberg and also the DAC areas in Styria increasingly deal with this old colour mutant of Pinot noir. In general, the distribution of the variety in Austria is relatively even. This means that Pinot blanc can be found everywhere, but it is not the most important grape variety anywhere and therefore plays an economically subordinate role in comparison with Gr¨ uner Veltliner [1]. However, with a total cultivated area of 1,992 ha hectares, Austria is the third largest Pinot Blanc-producing country in the world (14,834 hectares) [2]. The concept of wine typicity in terms of the terroir is of the opinion that the oenological practices, in addition to the grape variety and the geographical origin, make an important contribution to the final expression in the finished wine [3]. There are only a few scientific publications on the typicity of Pinot blanc wines and no papers that have dealt with the terroir for Pinot blanc production. Wines of this variety have always been considered rich in content and long-lasting. The typical aroma of Pinot blanc is described with pear, apple, quince, banana, apricot, caramel and citrus notes. For mature wines, bread flavours can be recognized. A longer bottle maturation allows the wine to gain in density and structure. The neutral aroma is considered to be less than trendy and the grape variety definitely does not have a youthful image [4]. Nevertheless, the wines tend to be in the upper quality and price range and are in great demand. In one study, 106 people (24 consumers, 34 winegrowers, 48 official wine tasters) in Austria were asked about the colour, flavour and taste characteristics of a typical Austrian Pinot blanc. The results showed that the terms ripe pomaceous fruit and nut flavours are most associated with Austrian Pinot blanc. For some wine categories, such as the reserve, wood and vanilla flavours play a crucial role [5]. Controversially, German Pinot blanc wines are often associated with the term pineapple [6]. Pinot blanc wines from South Tyrol are described with the flavour descriptors apple, pear, citrus notes, green notes, occasionally quince and exotic fruit, and in exceptional cases with spicy and nutty notes [7]. Furthermore, it was found that there is a positive correlation between wine quality, typicity and the perceived pear aroma of the Austrian Pinot blanc wines [8]. The perceived pear aroma is difficult to describe, as the studies [810] show. With the representation of sensory- analytical typograms of the pear aroma, a simple model for the description of the pear aroma has been demonstrated © The Authors, published by EDP Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/).

Transcript of Austrian Pinot blanc wines: Typicity, wine styles and the influence … · ethyl dodecanoate,...

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Austrian Pinot blanc wines: Typicity, wine styles and theinfluence of different oenological decisions on the volatileprofile of wines

    C. Philipp, S. Sari, P. Eder, E. Patzl-Fischerleitner, and R. Eder

    Höhere Bundeslehranstalt und Bundesamt für Wein- und Obstbau, Wienerstraße 74, 3400 Klosterneuburg, Austria

    Abstract. Pinot blanc is found in all wine regions of Austria. However, it plays an economically subordinaterole compared to the most important white grape variety, Grüner Veltliner. The concept of wine typicityand terroir is that the geographical origin and oenological practices, along with the grape variety, make animportant contribution to the final expression of the finished wine. The purpose of this study was to discussdifferent styles of Austrian Pinot blanc wines and to discuss the various wine production decisions in terms ofwine stylistics and aromatic profile. 131 commercial Austrian wine samples of the Pinot blanc variety ofthe 2015 to 2017 vintages were collected and the manufacturer’s practices were determined by means of aquestionnaire related to the wine styles. Using various GC-SIM-MS methods, more than 100 essential volatilesubstances were quantified. A trained panel, an expert panel and consumers evaluated the wines based ontypicity and quality. 15 typical wines were then assessed on a sensory basis by means of predetermined aromaand wine styles. The four Austrian Pinot blanc wine styles previously discussed and presented by the sameauthors (citrus (1st style), green apple and green pear (2nd style), ripe yellow apple, quince, cooked pear, breadand honey notes (3rd style), as well as intensive oak notes (4th style)) were able to be partially reproduced bythe trained panel. The following three wine styles could be better understood by the consumers: fresh fruity(style A), complex aroma with or without moderate wood (style B) and lots of wood aroma (style C). Thesestyles (A–C) were able to withstand a sensory examination and a discriminant analysis by substance. Anoenological guideline could help the producers to achieve the respective style. Austria’s wine-growing areais probably too small and the importance of the Pinot blanc variety for the individual areas still too low tosupport the terroir concept for this grape variety.

    1. Introduction

    Pinot blanc is an old grape variety with some fans.Interestingly, it is experiencing an unexpected renaissancein Central Europe, especially in Germany, South Tyrol andAustria. In Austria, specialisations are to be found wherePinot blanc has now been approved as DAC (DistrictusAustriae Controllatus). Accordingly, the regions aroundthe Leithaberg and also the DAC areas in Styriaincreasingly deal with this old colour mutant of Pinot noir.In general, the distribution of the variety in Austria isrelatively even. This means that Pinot blanc can be foundeverywhere, but it is not the most important grape varietyanywhere and therefore plays an economically subordinaterole in comparison with Grüner Veltliner [1]. However,with a total cultivated area of 1,992 ha hectares, Austriais the third largest Pinot Blanc-producing country in theworld (14,834 hectares) [2].

    The concept of wine typicity in terms of the terroir isof the opinion that the oenological practices, in additionto the grape variety and the geographical origin, makean important contribution to the final expression inthe finished wine [3]. There are only a few scientificpublications on the typicity of Pinot blanc wines andno papers that have dealt with the terroir for Pinotblanc production. Wines of this variety have always beenconsidered rich in content and long-lasting. The typical

    aroma of Pinot blanc is described with pear, apple, quince,banana, apricot, caramel and citrus notes. For maturewines, bread flavours can be recognized. A longer bottlematuration allows the wine to gain in density and structure.The neutral aroma is considered to be less than trendyand the grape variety definitely does not have a youthfulimage [4]. Nevertheless, the wines tend to be in theupper quality and price range and are in great demand.In one study, 106 people (24 consumers, 34 winegrowers,48 official wine tasters) in Austria were asked aboutthe colour, flavour and taste characteristics of a typicalAustrian Pinot blanc. The results showed that the termsripe pomaceous fruit and nut flavours are most associatedwith Austrian Pinot blanc. For some wine categories, suchas the reserve, wood and vanilla flavours play a crucial role[5]. Controversially, German Pinot blanc wines are oftenassociated with the term pineapple [6]. Pinot blanc winesfrom South Tyrol are described with the flavour descriptorsapple, pear, citrus notes, green notes, occasionally quinceand exotic fruit, and in exceptional cases with spicy andnutty notes [7]. Furthermore, it was found that there is apositive correlation between wine quality, typicity and theperceived pear aroma of the Austrian Pinot blanc wines [8].The perceived pear aroma is difficult to describe, as thestudies [8–10] show. With the representation of sensory-analytical typograms of the pear aroma, a simple model forthe description of the pear aroma has been demonstrated

    © The Authors, published by EDP Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0(http://creativecommons.org/licenses/by/4.0/).

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    and various influencing parameters on the composition ofthe typograms discussed [8–10].

    Just as there are few papers on the typicity of Pinotblanc wines, there are even fewer scientific publicationson the theme of Pinot blanc wine styles. Only [11] havediscussed wine styles of typical Pinot blanc wines. The15 most typical wines from a total sample number of46 Pinot blanc of the 2016 vintage and Ausrian originwere evaluated by Napping®- method with 21 trainedexperts, 34 wine producers and 21 wine consumers.The results showed that the 15 wines were divided bythe panel members into 4 wine styles. Pinot blanc withthe descriptors ripe yellow fruit, bread, honey and briochewere grouped together. Furthermore, Pinot blanc clusteredwith green fruit flavours like green apple, green pear.As a third group Pinot blanc with citrus flavours wereclustered in a group. The fourth Pinot blanc style describedwere wines with vanilla, oak and or nut flavours. AKruskal-Wallis test revealed significant differences (p <0.05) between these different styles in perceived overallquality, perceived typicity, total alcohol, ethyl hexanoate,ethyl octanoate, methyl (E) -2,6-octadienoate, methyl andethyl hexanoate content, ethyl decadienoate (isomers),ethyl dodecanoate, methyl vanillate, cis-whisky lactone,1-butanol, (z) -linalool oxide and diacetyl [11].

    The aim of this study was to test whether thesefour wine styles proposed by [11] are sensorially andanalytically reproducible, or whether other to be definedwine styles are better suited for Austrian Pinot blancwines. Furthermore, the oenological decisions made by thecorresponding wine producers to achieve the wine styleswere evaluated. As [12] proposed describing the typicity ofwines both from the perspective of the producers and fromthe field of vision of the consumers, the typicity and styleof the Austrian Pinot blanc wines should be consideredfrom these poles in this paper.

    2. Material and methods2.1. Samples

    131 different Pinot blanc wines from the years 2015to 2017 were collected from a total of 64 wineriesin Austria. The wines came from all relevant wineregions in the federal states of Burgenland, Vienna, LowerAustria and Styria. Depending on the wine production,the Pinot blancs were sourced from the producers afterharvest from April to October and stored at 4 ◦C untilanalysis. The wines were described by the winemakersas typical of the variety and vintage. Metadata about thewines was then collected using an internet questionnaire(www.soscisurvey.de). A total of 13 questions wereasked about sugar content, maceration time, pressfractions, clarification variants, must finings, spontaneousfermentation, pure cultured yeast, fermentation tank,fermentation temperature, maturation choice, months ofyeast contact, barrel storage and filtration.

    2.2. Sensory analyses

    To discuss different wine styles, sensory experiments wereconducted with a consumer panel (n = 15) and a panel ofpeople trained for the specific issues (n = 12). In addition,an expert panel (n = 6) was available.

    The trained taster panel consisted of 12 people aged 24to 46 years. Of these, 5 were female and 7 male, who wereinvolved in wine production, analysis and evaluation in avariety of ways in everyday life and in their profession.The tasters were employees of the Federal College andResearch Center of Viticulture and Pomology, studentsof agricultural science or oenology, employees of theUniversity of Natural Resources and Life Sciences andwinegrowers. The training of the panel was carried out insix units of 1.5 hours, so that the parameters, that wereimportant for the assessment of the given wine styles,were imprinted. Possible Pinot blanc styles were furtherdiscussed in three units of 1.5 hours each.

    The consumer panel consisted of 15 people who wereto correspond to an average wine consumer and were notfurther defined. The expert panel consisted of personsqualified to participate in official wine tasting and havemany years of experience in the sensory evaluation ofwines. They said they had some expertise in evaluation ofPinot blanc too. The consumer panel and the expert panelwere not given training.

    The tasting sessions were held at the Federal Collegeand Research Center of Viticulture and Pomology inKlosterneuburg in accordance with the guidelines of anISO17025 accredited laboratory for quality wine testing.For the evaluation of the quality and typicity of thewines, the expert panel and the trained panel, but not theconsumer panel, were interviewed together. The qualitywas evaluated in five batches (each with a maximum of15 wines, anonymous and randomized) over two tastingsessions per year, according to a 20-point scheme, and theevaluation of the typicity of the wines using unstructuredscales (10 cm) in duplicate. The tastings took place in theautumn of the year following the harvest.

    In the course of this study, only 5 typical wines peryear were supposed to be used for further sensory tests.The trained panel and the consumer had to rate 5 typicalwines of the three vintages (15 wines in total) with regardto the type of wine. This happened in autumn of 2018. Allwines were checked before tasting for any ageing defectsand replaced in case of abnormalities by the next rankedwine. This happened in 3 cases. In the evaluation of thewine styles, the vintages were not evaluated separately dueto their different maturity, but in three rounds, each with10 wines (mixed from the three vintages, also possibledouble nominations, randomized and anonymous). Eachtaster had to evaluate 30 wines. The tasters were invitedto taste one Pinot blanc after the other and then put themtogether in groups. They had to form at least 2 differentgroups but a maximum of 7 groups, whereby one winecould also be a group. Once the group formation had beencompleted, the trained panel had to label the groups createdand describe them using the given terms. The followingflavours were available: apricot, vineyard peach, greenapple, overripe/ripe apple, pear, walnut, almond, hazelnut,grapefruit, lemon, pineapple, banana, mango, figs, applecompote, elderflower, wild flowers, fresh grass, asparagus,vanilla, cinnamon, pepper, coconut, burnt sugar, honey,oak, burnt wood, bacon, leather, sauerkraut, yoghurt,biscuit, honey, fresh bread and chocolate. The consumerpanel only had to divide the groups into fruity and light,fruity and complex and complex with clear wood andvanilla notes.

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  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    2.3. Analytics

    For the determination of the basic parameters of the wines,FT-IR analyses according to OIV/OENO Resolution390/2010 were carried out by means of a FOSS WineScan(FT 120 Reference Manual, Foss, Hamburg, Germany)[13].

    Of the 15 selected wines, a total of 102 flavours ofthe aromatic group, esters, higher alcohols, carboxylicacids, carbonyl compounds, lactones, volatile phenols, freemonoterpenes and free C13-norisoprenoids were analysedin duplicate using 6 different GC-SIM-MS methods. Thecontent of decadienoic acid ethyl ester was determinedaccording to [9] by means of HS-SPME-GC-SIM-MS. Thequantification of the 14 relevant free monoterpenes wascarried out according [14]. In addition, 32 esters weredetermined by Stable Isotope Dilution Assay HeadspaceSolid-Phase Microextraction Gas Chromatography MassSpectroscopy (SIDA-HS-SPME-GC-MS). The methodwas developed by [15] especially for Pinot blancwines. [16] was used to quantify the C13-norisoprenoids(1,1,6-trimethyl-1,2-dihydronaphthalene, and vitispirane).The determination of the volatile phenols and lactoneswas carried out according to [17]. The determinationof the main quantitative flavourings such as relevanthigher alcohols, relevant short and mid-chain carboxylicacids, carbonyl compounds and ester compounds werecarried out by means of a partial SIDA-HS-SPME-GC-MS method. An Agilent Technologies 6890 N NetworkGC system, an Agilent 5975 Inert Mass SelectiveDetector (both Agilent Technologies, Santa Clara USA)and a CTC Analytics autosampler (Zwingen, Switzer-land) with a 65µm, polydimethyl-siloxane/divinylbenzene(PDMS/DVB), fused silica/SS fibre core (SUPLECO, PA,USA) fibre were used. 1 g salt (NaCl) was weighed intoa 20 ml vial and 2 ml milli-Q water added. Then 1 mlof the sample was pipetted to it. 50µl each of the threeinternal standard solutions (acid standard: butanoic acid-d7, hexanoic acid-d12, ester standard: ethylhexanaote-d5,ethyloctanoate-d5 and alcohol standard (butanol-d10 andhexanol-d13); in 2 butanone; nominal concentration 200µg/l) were pipetted to it. The sample was pre-incubated inthe autosampler unit (PAL) for one minute, then stirred atan incubation temperature of 30 ◦C with a magnetic stirrerat 500 rpm and extracted for 30 minutes. Subsequently,the fibre was injected into the gas chromatograph in“splitless mode” and the extracted sample was desorbedfor 35 minutes at 245 ◦C. The GC instrument parameterswere the same as in [18]. The samples were measuredin single-ion mode (SIM). The evaluation was madeusing the relative ratio of the peak area of the sampleto the peak area of the internal standard. The followingstandards were analysed and the m/z ratios used forthe quantification and the retention times are given inthe bracket: 1-propanol (31, RT: 9.312 min), isobutanol(42, RT: 11.487 min), isoamyl alcohol (70, RT: 16.825 min),1-butanol (56, RT: 13.888 min), (Z) -3-hexen-1-ol (82,RT: 24.817) diacetyl (43, RT: 6.932 min), ethyl lactate(45, RT: 22.875 min) ethyl acetate (61, RT: 4.687 min),ethyl propanoate (57, RT: 6.364 min), isovaleric acid(60, RT: 36.433 min), 1-hexanol (69: RT: 23.495 min),propionic acid (74, RT: 33.460 min), butyric acid (60, RT:35.203 min), hexanoic acid (60, RT: 42.868 min), octanoicacid (115, RT: 50.053 min), isobutyric acid (43, RT:

    Table 1. Determined typicity, quality and origin of the wines usedfor the wine style evaluation, ranked according to the typicity.

    No. Vintage Typicity Quality Wine region1 2015 8.4 15.6 Leithaberg2 2016 7.9 17.4 Kamptal7 2016 7.7 16.0 Klosterneuburg

    11 2016 7.5 15,4 Wagram12 2017 7.5 15.6 Kamptal13 2016 7.4 15.9 Wachau8 2015 7.3 17.2 South Styria

    14 2017 7.3 14.7 Leithaberg9 2017 7.2 16.2 Thermenregion6 2015 7.2 15.2 Weinviertel

    10 2016 7.0 15.7 South Styria3 2016 6.9 15.3 Thermenregion

    15 2015 6.9 13.9 South Styria4 2015 6.6 14.8 Weinviertel5 2017 6.4 15.0 Vienna

    32.456 min), decanoic acid (129, RT: 56.551 min), diethylsuccinates (101, RT: 36.679 min) and benzyl alcohol (108,RT: 43.932 min).

    2.4. Statistics

    The statistical evaluation was done with SPSS 22.0(IBM, Armonk, USA). First, the data was subjected to adescriptive analysis and checked for normal distribution.The analytical data was not normally distributed. Themedian value was used as the central value. The flavouranalyses and basic parameters of the wines were subjectedto a common principal component analysis. The scatterand charge diagram were presented in the form of aPCA biplot representation and presents the percent ofthe covered variance of the scattering in the two axes.The diagram was further divided into four quadrants. Allanalytical data was used to calculate the first and secondfactors. The same statistical module was used for theevaluation of the sensory evaluation by the trained paneland the consumer panel as well as the evaluation of theonline questionnaire [19].

    3. Results

    3.1. Typicity and quality of the wines

    In the course of the study, 131 Pinot blanc wines from theyears 2015 to 2017 were assessed by an expert commissionand a trained panel according to their typicity and overallquality. The average typicity of the 131 wines obtainedfrom both commissions varied from 1.83 to 8.41 ona 10 cm scale (0 not typical and 10 very typical) andthe overall quality from 9.73 (defective wine) to 17.44(excellent wine). There was a strong positive correlationbetween the results of the two commissions (r > 0.8),because of they were evaluated together. Table 1 shows thetypicity, quality and origin of the 15 typical wines selectedfor the evaluation of the wines. The alcohol content variedfrom 12.2 to 14.8% vol. The typicity of the wines wasgreater than 6.4 cm, the quality greater than 13.9 points.

    3

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Figure 1. Principal component analysis of the results of theflavour analyses and basic parameters of the 15 evaluated wines(1st quadrant top right, 2nd quadrant top left, 3rd quadrant bottomleft, 4th quadrant bottom right).

    3.2. Results of the aroma analyses

    102 volatile substances and some basic parameterswere analysed in the 15 wines used for the evaluation(Table 2–4). A statistical evaluation of the results wasachieved by means of principal component analysis(Fig. 1). It can be seen that wines 11, 13, 14 and 12cluster together in the first quadrant. These wines showedconcentrations higher than the median values for theamounts of the ethyl esters, methyl esters and isoamylesters. Also, the values for the volatile phenols and oaksubstances are greater than the median concentrations,although not due to high levels of vanillin. Wine 2 differsfrom the other wines in the high concentrations of volatilephenols and oak substances, especially vanillin. But it alsoshows high concentrations of ethyl esters, methyl estersand aromatic esters. Wines 10, 8 and 9 show high levelsof free monoterpenes and cluster together in the secondquadrant. Unlike the cluster in Quadrant 1, the wines inQuadrant 2 are heterogeneous in terms of concentrationsof esters and volatile phenols. Wine 9 has undergone acomplete malolactic fermentation, which is why the ethyllactate concentrations in this wine are very high comparedto wines 10 and 8. The wines of Quadrant 3 show low ethylester concentrations but are heterogeneous as far as thevolatile phenols and some other flavourings are concerned.Wines 3 and 15 show high ethyl acetate and diacetylconcentrations. On the basis of the lactic acid values,wines 5, 7, 4, 1, 3 and 15 show (results not presented) anincipient acid degradation or an acidification with lacticacid. Sample 5 had more residual sugar at 6 g/l than theother samples (

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

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    32.2

    33.9

    25.6

    22.3

    Sum

    free

    mon

    oter

    pene

    s(µ

    g/l)

    41.4

    50.2

    166.

    249

    .758

    .352

    .339

    .223

    6.6

    149.

    318

    3.1

    61.2

    45.1

    65.7

    6471

    .961

    .2H

    ighe

    ral

    coho

    ls(m

    g/l)

    1-Pr

    opan

    ol34

    .35

    37.5

    138

    .81

    34.2

    523

    .44

    27.6

    530

    .29

    29.4

    148

    .83

    44.2

    150

    .84

    31.0

    627

    .48

    49.5

    818

    .23

    34.2

    5Is

    obut

    anol

    22.5

    637

    .92

    25.2

    840

    .16

    35.2

    032

    .49

    24.9

    524

    .97

    19.2

    519

    .99

    33.4

    434

    .85

    31.1

    732

    .16

    32.2

    232

    .16

    Isoa

    myl

    alco

    hol

    145.

    7015

    7.50

    159.

    2017

    9.10

    125.

    6015

    5.00

    101.

    0013

    2.60

    121.

    4014

    8.50

    163.

    2011

    6.70

    147.

    3018

    8.90

    104.

    0014

    7.36

    1-B

    utan

    ol0.

    170.

    110.

    120.

    190.

    140.

    130.

    130.

    160.

    330.

    160.

    360.

    160.

    150.

    380.

    090.

    16(Z

    )-3-

    hexe

    n-1-

    ol0.

    060.

    080.

    100.

    110.

    050.

    080.

    040.

    130.

    050.

    090.

    090.

    110.

    150.

    110.

    170.

    091-

    Hex

    anol

    1.66

    0.88

    1.80

    0.66

    0.44

    2.35

    0.73

    1.54

    1.78

    3.08

    1.00

    1.40

    1.57

    1.29

    0.99

    1.40

    Sum

    high

    eral

    coho

    ls(m

    g/l)

    204.

    523

    422

    5.31

    254.

    4718

    4.87

    217.

    715

    7.14

    188.

    8119

    1.64

    216.

    0324

    8.93

    184.

    2820

    7.82

    272.

    4215

    5.70

    207.

    82Vo

    latil

    efa

    ttyac

    ids

    (mg/

    l)Pr

    opan

    oic

    acid

    1.30

    0.87

    0.80

    2.36

    1.08

    1.47

    1.87

    1.08

    1.96

    1.75

    1.23

    1.20

    1.95

    1.57

    1.99

    1.47

    But

    yric

    acid

    0.96

    1.03

    0.93

    1.02

    0.44

    1.01

    1.90

    1.91

    1.05

    1.06

    1.82

    1.72

    0.23

    2.10

    1.82

    1.05

    Isob

    utyr

    icac

    id1.

    370.

    680.

    920.

    900.

    881.

    171.

    541.

    750.

    960.

    921.

    380.

    961.

    330.

    601.

    830.

    96Is

    oval

    eric

    acid

    0.32

    0.37

    0.48

    0.44

    0.22

    0.40

    0.36

    0.46

    0.34

    0.40

    0.56

    0.29

    0.25

    0.46

    0.14

    0.37

    Hex

    anoi

    cac

    id0.

    291.

    562.

    622.

    291.

    332.

    562.

    533.

    341.

    821.

    781.

    491.

    412.

    021.

    491.

    161.

    78O

    ctan

    oic

    acid

    5.37

    6.29

    3.95

    5.16

    5.41

    3.53

    4.42

    6.06

    3.33

    3.70

    6.13

    5.67

    6.86

    3.84

    6.95

    5.37

    Dec

    anoi

    cac

    id0.

    822.

    316.

    230.

    903.

    041.

    091.

    512.

    861.

    581.

    042.

    041.

    942.

    641.

    852.

    021.

    94Su

    mvo

    lati

    lefa

    tty

    acid

    s(m

    g/l)

    10.4

    313

    .11

    15.9

    313

    .07

    12.4

    11.2

    314

    .13

    17.4

    611

    .04

    10.6

    514

    .65

    13.1

    915

    .28

    11.9

    115

    .91

    13.1

    1E

    thyl

    este

    rs(µ

    g/l)

    Eth

    ylac

    etat

    e(m

    g/l)

    44.1

    035

    .50

    78.4

    051

    .10

    45.5

    055

    .70

    76.2

    037

    .30

    47.6

    041

    .90

    29.1

    037

    .20

    31.5

    032

    .80

    88.9

    037

    .20

    Eth

    ylpr

    opan

    oate

    197.

    070

    .010

    6.0

    84.0

    153.

    084

    .087

    .927

    618

    1.0

    120.

    373

    .725

    2.3

    167.

    923

    2.7

    328.

    915

    3.0

    Eth

    ylbu

    tano

    ate

    397.

    566

    5.6

    247.

    147

    0.9

    498.

    542

    0.0

    349.

    052

    9.9

    257.

    032

    4.6

    389.

    054

    2.8

    523.

    942

    7.9

    231.

    342

    0.0

    Eth

    ylis

    oval

    erat

    e9.

    113

    .310

    .010

    .66.

    613

    .15.

    718

    .512

    .812

    .916

    .512

    .917

    .710

    .010

    .712

    .8E

    thyl

    vale

    rate

    0.9

    0.9

    0.4

    0.6

    0.6

    0.4

    0.6

    1.1

    1.0

    0.8

    0.7

    0.9

    1.0

    1.2

    0.7

    0.8

    Eth

    ylhe

    xano

    ate

    837.

    610

    79.8

    485.

    877

    8.0

    1129

    .360

    8.2

    770.

    783

    5.8

    561.

    656

    6.6

    927.

    277

    8.8

    1079

    .981

    5.3

    550.

    777

    8.8

    Eth

    ylhe

    ptan

    oate

    0.7

    1.4

    0.7

    0.9

    0.7

    0.2

    0.3

    0.6

    0.4

    0.4

    0.8

    0.6

    0.6

    0.8

    0.2

    0.6

    Eth

    yloc

    tano

    ate

    3655

    .439

    76.5

    1673

    .124

    63.9

    1843

    .118

    07.3

    2056

    .719

    32.7

    1908

    .418

    44.7

    2687

    .521

    15.0

    2719

    .425

    68.8

    1550

    .420

    85.9

    Eth

    yldo

    deca

    noat

    e71

    .749

    .119

    .146

    .263

    .110

    6.7

    66.5

    65.8

    90.0

    84.8

    56.2

    76.4

    62.6

    138.

    266

    .166

    .1E

    thyl

    tetr

    adec

    anoa

    te15

    .816

    .815

    .413

    .417

    .014

    .912

    .711

    .521

    .014

    .215

    .815

    .87.

    118

    .323

    .715

    .8E

    thyl

    hexa

    deca

    noat

    e45

    .352

    .124

    .538

    .147

    .714

    .938

    .530

    .463

    .642

    .947

    .833

    .823

    .966

    .150

    .042

    .9E

    thyl

    deca

    noat

    e16

    25.9

    1516

    .361

    1.5

    1239

    .815

    35.9

    1761

    .014

    50.4

    1494

    .823

    98.2

    1616

    .818

    01.3

    1743

    .615

    80.5

    3186

    .115

    18.4

    1580

    .5Su

    met

    hyle

    ster

    sw

    itho

    utet

    hyla

    ceta

    te68

    56.9

    7441

    .831

    93.6

    5146

    .452

    95.5

    4830

    .748

    39.0

    5197

    .154

    95.0

    4629

    .060

    16.5

    5572

    .961

    84.5

    7465

    .443

    31.1

    5295

    .5

    5

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Tabl

    e3.

    Con

    tent

    (mea

    nof

    two

    mea

    sure

    men

    ts)o

    fthe

    met

    hyle

    ster

    s,is

    oam

    yles

    ters

    ,aro

    mat

    ices

    ters

    ,est

    ers

    ofun

    satu

    rate

    dfa

    ttyac

    ids,

    acet

    ates

    ofhi

    gher

    alco

    hols

    ,mix

    edan

    dot

    here

    ster

    sin

    clud

    ing

    med

    ian

    valu

    ein

    the

    15w

    ines

    stud

    ied.

    Met

    hyle

    ster

    s(µ

    g/l)

    1.0

    2.0

    3.0

    4.0

    5.0

    6.0

    7.0

    8.0

    9.0

    10.0

    11.0

    12.0

    13.0

    14.0

    15.0

    Med

    ian

    Met

    hyli

    sova

    lera

    te<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1M

    ethy

    lhex

    anoa

    te1.

    31.

    31.

    20.

    71.

    20.

    60.

    61.

    11.

    00.

    92.

    01.

    52.

    81.

    50.

    71.

    2M

    ethy

    loct

    anoa

    te0.

    90.

    80.

    70.

    60.

    90.

    50.

    81.

    01.

    30.

    91.

    00.

    91.

    70.

    90.

    40.

    9M

    ethy

    ldec

    anoa

    te0.

    80.

    50.

    40.

    40.

    60.

    70.

    81.

    11.

    91.

    41.

    00.

    91.

    51.

    20.

    50.

    8M

    ethy

    ldod

    ecan

    oate

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    Met

    hylt

    etra

    deca

    noat

    e<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1Su

    mm

    ethy

    lest

    ers

    (µg/

    l)3.

    02.

    62.

    31.

    72.

    71.

    82.

    23.

    24.

    23.

    24.

    03.

    36.

    03.

    61.

    63.

    0Is

    oam

    yles

    ters

    (µg/

    l)Is

    oam

    ylac

    etat

    e23

    02.5

    1689

    .583

    0.2

    1147

    .325

    70.9

    1488

    .198

    6.1

    2975

    .321

    16.0

    1634

    .021

    03.7

    1737

    .221

    82.6

    2548

    .944

    1.2

    1737

    .2Is

    oam

    ylbu

    tano

    ate

    0.2

    0.5

    0.5

    0.5

    0.4

    0.4

    0.4

    0.4

    0.4

    0.4

    0.6

    0.6

    0.7

    0.4

    0.3

    0.4

    Isoa

    myl

    isov

    aler

    ate

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    Isoa

    myl

    hexa

    noat

    e2.

    73.

    01.

    42.

    62.

    91.

    61.

    51.

    91.

    81.

    81.

    81.

    52.

    32.

    21.

    01.

    8Is

    oam

    yloc

    tano

    ate

    1.9

    3.5

    1.3

    2.6

    1.9

    2.7

    3.4

    4.0

    2.4

    2.5

    4.9

    5.4

    6.7

    11.1

    3.9

    3.4

    Sum

    isoa

    myl

    este

    rs(µ

    g/l)

    2307

    .316

    96.5

    833.

    411

    5325

    76.1

    1492

    .899

    1.4

    2981

    .621

    20.6

    1638

    .721

    1117

    44.7

    2192

    .325

    62.6

    446.

    417

    44.7

    Aro

    mat

    ices

    ters

    (µg/

    l)E

    thyl

    benz

    oate

    0.2

    0.4

    0.2

    0.2

    0.8

    0.2

    0.2

    0.2

    0.3

    0.4

    0.3

    0.2

    0.3

    0.3

    0.3

    0.3

    Hex

    ylph

    enyl

    acet

    ate

    0.7

    0.4

    2.2

    0.5

    1.0

    0.6

    0.6

    0.7

    0.7

    0.4

    0.2

    0.4

    0.3

    0.3

    0.9

    0.6

    Eth

    ylsa

    licyl

    ate

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    n.d.

    Eth

    ylph

    enyl

    acet

    ate

    0.9

    5.0

    1.7

    2.0

    1.2

    0.6

    0.6

    2.4

    1.2

    1.0

    1.3

    2.0

    2.3

    2.2

    1.3

    1.3

    Sum

    arom

    atic

    este

    rs(µ

    g/l)

    1.8

    5.8

    4.1

    2.7

    3.0

    1.4

    1.4

    3.3

    2.2

    1.8

    1.8

    2.6

    2.9

    2.8

    2.5

    2.6

    Est

    ers

    from

    unsa

    tura

    ted

    acid

    s(µ

    g/l)

    Eth

    yl(E

    )-2-

    dece

    noat

    en.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.n.

    d.E

    thyl

    (E,Z

    )-2,

    4-de

    cadi

    enoa

    te1.

    81.

    61.

    61.

    01.

    61.

    00.

    30.

    80.

    30.

    40.

    10.

    20.

    20.

    20.

    20.

    4H

    ighe

    ral

    coho

    lace

    tate

    s(µ

    g/l)

    Isob

    utyl

    acet

    ate

    113.

    415

    2.8

    47.1

    150.

    711

    7.1

    124.

    710

    0.3

    160.

    311

    3.0

    157.

    710

    2.7

    63.0

    57.3

    77.8

    36.3

    113.

    02-

    Met

    hylb

    utyl

    acet

    ate

    324.

    849

    .323

    .060

    .759

    .611

    9.1

    78.9

    238.

    016

    9.3

    130.

    722

    5.6

    109.

    611

    1.4

    138.

    743

    .311

    1.4

    Hex

    ylac

    etat

    e28

    4.2

    192.

    365

    .626

    1.8

    140.

    917

    7.3

    36.5

    216.

    414

    6.9

    410.

    823

    6.3

    168.

    219

    1.7

    148.

    939

    .817

    7.3

    Oct

    ylac

    etat

    e0.

    51.

    30.

    71.

    00.

    51.

    31.

    21.

    31.

    51.

    71.

    71.

    42.

    11.

    80.

    91.

    3Su

    mhi

    gher

    alco

    hola

    ceta

    tes

    (µg/

    l)72

    2.9

    395.

    713

    6.4

    474.

    231

    8.1

    422.

    421

    6.9

    616

    430.

    770

    0.9

    566.

    334

    2.2

    362.

    536

    7.2

    120.

    339

    5.7

    Mix

    edan

    dot

    her

    este

    rs(µ

    g/l)

    Isob

    utyl

    prop

    iona

    te27

    .063

    .79.

    520

    .46.

    123

    .110

    .029

    .222

    .722

    .815

    .322

    .025

    .117

    .314

    .822

    .0B

    utyl

    isob

    utan

    oate

    0.4

    0.0

    0.0

    0.1

    0.0

    0.3

    0.3

    0.7

    0.8

    0.5

    0.6

    0.3

    0.3

    0.5

    0.3

    0.3

    Pent

    ylbu

    tano

    ate

    0.1

    0.3

    0.1

    0.2

    0.2

    0.2

    0.2

    0.2

    0.3

    0.2

    0.2

    0.3

    0.2

    0.2

    0.3

    0.2

    Hex

    ylis

    obut

    anoa

    te0.

    20.

    20.

    10.

    20.

    30.

    20.

    30.

    30.

    20.

    20.

    40.

    40.

    50.

    30.

    30.

    3Pr

    opyl

    isov

    aler

    ate

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    <0.

    1<

    0.1

    But

    ylbu

    tano

    ate

    15.1

    15.5

    12.0

    17.0

    12.0

    11.8

    11.8

    13.1

    13.2

    12.2

    11.8

    11.8

    12.0

    12.1

    13.0

    12.1

    Prop

    yloc

    tano

    ate

    1.3

    0.8

    0.7

    0.9

    1.5

    0.6

    0.9

    1.2

    2.2

    1.6

    1.7

    1.2

    1.2

    2.1

    0.4

    1.2

    Isob

    utyl

    octa

    noat

    e0.

    30.

    70.

    20.

    40.

    20.

    80.

    71.

    01.

    00.

    60.

    50.

    80.

    71.

    10.

    70.

    7E

    thyl

    lact

    ate

    1496

    5.0

    3428

    6.0

    3438

    9.0

    1798

    9.0

    1419

    6.0

    8610

    .010

    0350

    .096

    10.0

    1720

    40.0

    1026

    0.0

    1295

    5.0

    1477

    5.0

    1189

    0.0

    1354

    5.0

    6968

    0.0

    1419

    6.0

    Die

    thyl

    succ

    inat

    e12

    14.0

    2173

    .014

    33.0

    1127

    .091

    3.0

    2240

    .017

    60.0

    1160

    .019

    60.0

    1640

    .023

    44.0

    2088

    .019

    36.0

    1072

    .046

    08.0

    1760

    .0Su

    mm

    ixed

    and

    othe

    res

    ters

    (µg/

    l)16

    223.

    436

    540.

    235

    844.

    619

    155.

    215

    129.

    310

    887

    1021

    34.2

    1081

    5.7

    1740

    40.4

    1193

    8.1

    1532

    9.5

    1689

    9.8

    1386

    614

    650.

    674

    317.

    815

    992.

    8

    6

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Tabl

    e4.

    Con

    tent

    (mea

    nof

    two

    mea

    sure

    men

    ts)

    ofC

    13-n

    oris

    opre

    noid

    s,ca

    rbon

    ylco

    mpo

    unds

    ,vol

    atile

    phen

    ols

    and

    lact

    ones

    inth

    e15

    win

    esst

    udie

    d.

    C13

    -non

    oris

    opre

    noid

    g/l)

    1.0

    2.0

    3.0

    4.0

    5.0

    6.0

    7.0

    8.0

    9.0

    10.0

    11.0

    12.0

    13.0

    14.0

    15.0

    Med

    ian

    Viti

    spir

    ane

    1.1

    1.6

    3.8

    0.8

    0.5

    0.8

    1.1

    0.4

    0.9

    1.3

    0.8

    2.2

    4.4

    0.8

    0.6

    0.9

    1,1,

    6-T

    rim

    ethy

    l-1,

    2-di

    hydr

    onap

    htha

    lene

    0.4

    0.6

    0.8

    0.3

    0.3

    0.3

    0.5

    0.1

    0.4

    0.4

    0.4

    0.5

    0.9

    0.4

    0.1

    0.4

    Sum

    C13

    -nor

    isop

    reno

    ids

    (µg/

    l)1.

    52.

    24.

    61.

    10.

    81.

    11.

    60.

    51.

    31.

    71.

    22.

    75.

    31.

    20.

    71.

    3C

    arbo

    nylc

    ompo

    unds

    (µg/

    l)D

    iace

    tyl

    780.

    019

    9.0

    754.

    085

    5.0

    257.

    060

    1.0

    616.

    073

    6.0

    404.

    062

    9.0

    319.

    027

    4.0

    596.

    024

    8.0

    927.

    060

    1.0

    Vola

    tile

    phen

    ols

    and

    lact

    ones

    µg/

    l)Fu

    rfur

    al15

    6.2

    511.

    919

    7.6

    80.7

    37.4

    191.

    313

    9.0

    96.9

    141.

    616

    5.3

    355.

    224

    4.7

    613.

    231

    1.1

    80.0

    165.

    35-

    Met

    hylf

    urfu

    ral

    1.2

    8.3

    1.7

    0.4

    0.4

    1.9

    1.4

    1.3

    1.4

    1.7

    1.7

    1.3

    6.7

    3.6

    1.3

    1.4

    o-C

    reso

    l0.

    40.

    80.

    31.

    30.

    50.

    61.

    30.

    50.

    81.

    70.

    60.

    40.

    40.

    30.

    40.

    5m

    /p-C

    reso

    liso

    mer

    1.9

    3.6

    1.2

    1.3

    1.3

    0.9

    2.9

    1.1

    0.3

    1.9

    0.9

    1.1

    1.4

    1.2

    0.7

    1.2

    Gua

    iaco

    l1.

    36.

    80.

    71.

    52.

    24.

    63.

    32.

    62.

    54.

    71.

    71.

    50.

    91.

    22.

    02.

    04-

    Met

    hylg

    uaia

    col

    <0.

    12.

    0<

    0.1

    <0.

    1<

    0.1

    0.2

    0.3

    0.5

    0.2

    0.2

    0.7

    0.1

    0.1

    0.3

    0.0

    0.2

    4-E

    thyl

    guai

    acol

    0.4

    2.7

    0.3

    0.3

    0.3

    0.1

    0.8

    1.0

    0.4

    0.1

    0.2

    0.2

    0.0

    0.1

    0.1

    0.3

    (E)-

    whi

    sky

    lact

    one

    0.2

    1.2

    0.8

    0.1

    0.8

    0.8

    0.8

    1.5

    0.9

    0.7

    2.1

    1.2

    1.3

    1.3

    0.7

    0.8

    5-A

    ceto

    xym

    ethy

    l-2-

    fura

    ldeh

    yde

    30.5

    19.3

    30.4

    11.3

    23.2

    17.6

    10.1

    12.6

    12.0

    17.5

    24.6

    17.1

    27.1

    9.4

    18.5

    17.6

    4-V

    inyl

    guai

    acol

    85.8

    171.

    184

    .214

    7.2

    180.

    314

    6.3

    145.

    611

    8.5

    105.

    219

    7.7

    124.

    816

    4.5

    117.

    676

    .454

    .612

    4.8

    (Z)-

    whi

    sky

    lact

    one

    1.7

    2.0

    2.1

    1.8

    1.0

    2.5

    1.0

    3.3

    2.8

    1.1

    2.8

    0.2

    2.1

    0.5

    0.9

    1.8

    2,6-

    Dim

    etho

    xyph

    enol

    2.0

    23.7

    1.2

    2.5

    3.6

    0.7

    1.4

    0.7

    1.3

    0.6

    4.1

    3.9

    2.4

    2.8

    2.9

    2.4

    Eug

    enol

    0.7

    2.7

    3.8

    1.1

    0.9

    0.8

    0.9

    0.8

    2.3

    1.4

    1.5

    0.7

    1.0

    0.7

    3.2

    1.0

    2-M

    etho

    xy-4

    -pro

    pylp

    heno

    l<

    0.1

    0.3

    <0.

    10.

    3<

    0.1

    0.3

    0.6

    0.5

    0.3

    0.4

    <0.

    10.

    3<

    0.1

    <0.

    1<

    0.1

    0.3

    Van

    illin

    11.7

    136.

    621

    .510

    .111

    .722

    .015

    .919

    .527

    .310

    .527

    .015

    .725

    .053

    .714

    .219

    .5(Z

    )-is

    oeug

    neol

    2.6

    11.1

    7.4

    7.0

    4.8

    5.8

    4.1

    3.3

    5.4

    4.1

    9.1

    13.3

    12.5

    6.5

    7.2

    6.5

    (E)-

    isoe

    ugen

    ol0.

    53.

    31.

    01.

    30.

    80.

    30.

    40.

    30.

    30.

    21.

    10.

    80.

    50.

    80.

    50.

    5E

    thyl

    vani

    llin

    0.2

    0.2

    1.6

    0.2

    0.5

    0.5

    0.5

    0.5

    0.4

    0.7

    1.7

    1.4

    1.3

    1.3

    4.1

    0.5

    Ace

    tova

    nillo

    ne17

    .343

    .628

    .319

    .919

    .818

    .720

    .929

    .115

    .038

    .735

    .733

    .942

    .524

    .912

    .424

    .9D

    elta

    -dec

    alac

    tone

    4.5

    4.4

    3.1

    5.3

    4.9

    5.2

    3.1

    4.0

    2.9

    3.0

    4.8

    6.1

    6.3

    4.9

    2.5

    4.5

    Met

    hylv

    anill

    ate

    9.3

    9.1

    9.9

    9.3

    8.1

    10.2

    6.3

    11.4

    6.7

    22.9

    21.7

    18.1

    20.7

    15.9

    5.9

    9.9

    4-H

    ydro

    xy-3

    -met

    hoxy

    phen

    ylac

    eton

    e1.

    06.

    80.

    60.

    91.

    72.

    81.

    42.

    11.

    31.

    76.

    30.

    70.

    73.

    11.

    11.

    4E

    thyl

    vani

    llate

    7.6

    19.8

    7.5

    7.7

    21.5

    5.4

    5.2

    6.6

    6.5

    10.1

    34.5

    34.0

    31.0

    41.5

    13.7

    10.1

    4-A

    llyl-

    2,6-

    dim

    etho

    xyph

    enol

    0.6

    5.6

    0.9

    1.1

    2.6

    1.0

    0.9

    1.0

    0.5

    0.8

    3.6

    1.5

    2.0

    3.0

    0.9

    1.0

    Syri

    ngal

    dehy

    de12

    1.5

    75.8

    25.8

    36.3

    29.8

    107.

    870

    .023

    .065

    .261

    .514

    1.6

    86.0

    130.

    017

    6.6

    70.3

    70.3

    Ace

    tosy

    ring

    one

    6.0

    36.4

    6.7

    5.7

    5.9

    10.3

    7.0

    6.2

    8.2

    12.7

    28.7

    14.1

    20.7

    22.2

    16.0

    10.3

    Sum

    vola

    tile

    phen

    ols

    and

    lact

    ones

    (µg/

    l)46

    5.1

    1109

    .143

    8.6

    354.

    636

    455

    8.6

    445.

    134

    8.8

    411.

    756

    1.9

    836.

    766

    2.8

    1067

    .476

    3.3

    314.

    146

    5.1

    7

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Figure 2. Principal component analysis of sensory evaluationaccording to aromatic wine style by means of a trained panel(1st quadrant top right, 2nd quadrant top left, 3rd quadrant bottomleft, 4th quadrant bottom right).

    Figure 3. Principal component analysis of sensory evaluationaccording to aromatic wine style by means of a consumer panel.

    with maceration periods between 2 and 6 h and 6.7% ofthe wines had a maceration time of more than 10 h. Only33% of the wines contained press fractions from pressingsabove 1.2 bar. 40% of the operations sedimented the mustswithout enzyme and 20% by flotation. 20% of the mustswere not fined, however, only 6.7% of the musts werefined with combined products. 73.3% of the wines werefermented with pure cultured yeast, but only one winewas completely fermented spontaneously, the remainingwines were vinified combined. A wide variety of standardwhite wine yeasts (20%), special burgundy yeasts (46.7%)and other yeasts were used. No operation used yeasts torelease monoterpenes from sugar-bound precursors (aromayeasts). Only 20% of the wines were cleared quickly afterfermentation, the remaining wines were aged on the yeast,with 46.7% of the wines even having yeast contact timeslonger than 6 months. 33.3% of the wines were aged only

    in stainless steel tanks and only one wine 100% in smalloak barrels. 3 wines were not filtered sterile.

    The wines in the first quadrant generally had highersugar content, the maceration time was longer, and theyeast contact was more intense. Fermentation and storageof the wines took place in stainless steel tanks. The winesin the fourth quadrant were aged in wood. Wine 2 was alsofermented in wood. The vinification of wine 15 differedfrom all other wines in that it fermented spontaneously, andthe fermentation temperature was higher, and the wine wasnot sterilized. In wine 10, a combined fining was carriedout in the must. Wine 9 and wine 2 had a complete aciddecay. For the remaining wines, there were no distinctiveabnormalities. Standard yeasts were used, the fermentationtemperature was moderate, and no wood was traditionallyused in the vinification.

    4. InterpretationIn the course of this study, wines from the Pinot blancvarieties of the 2015 to 2017 vintages were tested tofind out whether the wine styles proposed by [11] (citrus(1st style), green apple and green pear (2nd style), ripeyellow apple, quince, cooked pear, bread and honey notes(3rd style), as well as intense oak notes (4th style)) aresensorially and analytically reproducible. Typicity wasconsidered by consumers, producers and wine experts. Itwas found that the four wine styles proposed by [11]could be partially recreated, but several gradations andtransitions between the styles existed, so that an absolutedistinction, with the exception of strong oak-maturedwines, was even difficult with a trained panel. The resultsof the consumer study showed that a distinction intothree groups fruity and light, fruity and complex, complexwood vanilla was more understood, but some incorrectassignments also happened here. On the other hand, theevaluation of the analytical results and the questionnaireafter the oenological decisions showed that the fruity andcomplex group also made it possible to include somewood aromas (partial ageing in oak barrels). Thus, thethree proposed wine styles, which were also understood byconsumers, could be named as follows: fresh fruity (styleA), complex aroma with or without moderate wood (styleB) and lots of wood aroma (style C). Of course, there werefine gradations between these types of wine. In connectionwith the analytical data and the data of the trained panel,it can thus be stated that wine 2 belongs to the complexaroma with lots of wood group, wines 1, 4, 5, 11, 12, 13,14 to the group complex aroma with or without moderatewood and wines 3, 6, 7, 8, 9, 10, 11 and 15 to freshlyfruity group. These styles (A–C) were able to withstanda discriminant analysis by substance (Fig. 4) and a sensoryexamination (Figs. 5 and 6).

    By interviewing the winegrowers and collectingmetadata for the vinification of the wines, one ofthem wanted to establish a direct connection betweenwinegrowers’ decisions on the aromatic profile of thewines and, finally, membership of a defined wine style.

    Due to the many choices and obviously greatvariability in the oenological processes, this connectionwas only partially successful. Sample 2 was 100%fermented in new oak and stored in oak, which is whymembership of style C, lots of wood aroma is clearlyreproducible. Wine 15 was fermented spontaneously at

    8

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Figure 4. Main component analysis of metadata collection:(sugar graduation (numeric in month); time of skin contact(ordinal: 0 whole-bunch pressing, 1: very short time skin contact,2: short skin contact (less than 2 hours), 3: moderate skin contact(2–5 hours), 4: long skin contact (5–10 hours), press fractions(ordinal: 1: only free run, 2: press fraction to 1.2 bar pressure,3: press fractions more than 1.2 bar included), child of mustclarification (ordinal: 0: no clarification, 1: natural sedimentationwithout addition of enzyme, 2: sedimentation with addition ofenzyme, 3: flotation), must finning (ordinal: 0: no must finning,1: addition of gelatine based products, 2: addition of caseinbased products, 3: combination of different finning products ormixed products); fermentation style (ordinal: 1: use of 100%commercial yeast, 2: part of the 3: after starting spontaneousfermentation commercial yeasts were added, 4: spontaneousfermentation); kind of yeast (ordinal: 1: standard white wineyeast (neutral), 2: special yeasts for Pinot blanc wines, 3: aromayeasts, 4: no yeast was added); fermentation vessel ordinal: 1:stainless steel, 2: combination of oak and stainless steel, 3: oak);fermentation temperature (1: cold fermentation (below 18 ◦C),2: moderate temperature (between 18 ◦C and 21 ◦C), 3: warmfermentation (above 21 ◦C)); vinification style (ordinal: 1: quickclarification after fermentation; 2: storage on the lees; 3: storageon the whole lees); month of sur lie (ordinal: 0: there is no storageon the lees, 1: short contact with lees (less than 2 months), 3:storage on the lees for 3 months, 4: long storage on the lees for 6months, 5: very long storage on the lees (more than 6 months));ageing on oak (ordinal: 0: no oak at all, 1: part of the wine inor on oak, 2: 100% of wine in or on oak); filtration (ordinal: 1:sterile filtration, 2: no sterile filtration).

    higher temperatures. Due to the higher ethyl acetateconcentration (Table 2), the overall fruitiness of the wine islikely to have increased. This connection is ambivalent. Atlow starting concentrations there is a positive correlationwith the quality, if a limit is exceeded (depending onthe type of wine, between 100–200 mg/l), a noticeableacetone smell leads to negative evaluations in the caseof tastings [21]. In contrast, wine 8, also with high ethylacetate concentrations, which was also numbered amongthe fruity wines, but was fermented with commercial whitewine yeasts. What connects the wines of the categoryfruity in general is short to no yeast contact times. Witha few exceptions (samples 11 and 12), the complex aromawith or without moderate wood wines had yeast contactafter fermentation, higher sugar content, longer maceration

    Figure 5. New wine styles (sensory data, consumer evaluation) –green: fresh fruity (style A), black: comples aroma with orwithout moderate wood (style B) and blue: lots of wood aroma(style C), without statistical evaluation of the style boundaries.

    Figure 6. New wine styles (sensory data, trained panelevaluation) – green: fresh fruity (style A: green apple, grapefruit,lemon), black: complex aroma with or without moderate wood(style B: pineapple, ripe apple, apricot, pear, walnut, maybe a bitoak and vanilla) and blue: lots of wood aroma (style C: vanilla,oak, bacon, cinnamon), without statistical evaluation of the styleboundaries.

    times and, as already discussed, in some cases a partialstorage in wooden casks.

    5. ConclusionThe selections of 15 typical Pinot blanc wines fromthe years 2015 to 2017 were grouped along the samelines as their aromatic profile and sensory evaluation inthree wine categories: fresh fruity (style A), complex

    9

  • BIO Web of Conferences 15, 02005 (2019) https://doi.org/10.1051/bioconf/20191502005

    42nd World Congress of Vine and Wine

    Figure 7. New wine styles (analytical data) – green: freshfruity (style A: higher concentration of free monoterpenes, ethylacetate), black: complex aroma with or without moderate wood(style B: higher concentration of middle and long chain ethylesters) and blue: lots of wood aroma (style C: high concen-tration of vanillin), without statistical evaluation of the styleboundaries.

    aroma with or without moderate wood (style B) and lotsof wood aroma (style C). Austria is a small but veryheterogeneous wine region. The terroir concept has alreadybeen implemented differently for some grape varietiessuch as Grüner Veltliner, Riesling and Blaufränkisch insome areas. However, Austria’s wine-growing area isprobably too small and the importance of the Pinot blancvariety for the individual areas still too low to supportthe terroir concept for this grape variety. Which is whythe proposed or another style profiling for the Pinotblanc producers could also be a first step towards bettermarketing and better consumer understanding. It should benoted at this point that the proposals for the style definitionhave been discussed with a relatively small number ofsamples in the course of this academic paper, so furtherstudies and evaluations would be useful. It is necessaryto further research on the question of which flavorscharacterize the respective wine style. An oenologicalguideline can help the producers to achieve the respectivestyle. Further research work should be invested in this tasktoo.

    We would like to thank the many people who took part in thesensory evaluations on a voluntary basis. This is especially truefor the 12 people of the trained tasting panel, who invested a lot oftime. We would also like to thank the wine producers who madetheir wines available for free.

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    10

    1 Introduction2 Material and methods3 Results4 Interpretation5 ConclusionReferences