Atlas of Light-CT images (biology and plants)
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Transcript of Atlas of Light-CT images (biology and plants)
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1 LLTECH 2011
LLTech
Light-CT Scanner
Atlas of images
Research applications
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2 LLTECH 2011
TABLE OF CONTENTS
COSMETOLOGY Page 3
CELLS Page 8
DEVELOPMENTAL BIOLOGY Page 13
PLANTS Page 25
RESEARCH APPLICATIONS
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3 LLTECH 2011
- In-vivo layer thickness measurement, cell count,epidermis/dermis junction analysis, for ageing
and hydration evaluation
- Skin model analysis
Back to Table of Contents
COSMETOLOGY
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4 LLTECH 2011
Stratum corneum Stratum spinosum
Stratum basale Reticular region
Epidermis
Dermis
En-face skin slicing shows structural and cellular details
Melanin
papillary caps
Keratinocytenuclei
Blood vessels
Collagen
Corneocytes
100 m 100 m
100 m100 m
LLTech 2012 Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland
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5 LLTECH 2011
Vertical reconstructions from en-face slicing:
Layer count and measurement
50 m
Stratum corneum
Stratum spinosum
Dermis
Human skin (in-vivo)
Skin model
Dalimier and Salomon Dermatology 224, 84-92 (2012)
Stack of images
-> vertical sections
Keratinocyte nuclei
Melanin caps at the
epidermis/dermis
junction
Blood vessel50 m
Evaluation of stratum
corneum thickness:
10 m
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Naevus imaging
500 microns
Dermal papillae
Hair follicule
100 m
50 m
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3D views
Wrinkle
Stratum corneum Stratum spinosum
Epidermis (separated by sodium bromide)
50 m
Reconstructed vertical slice
Dalimier and Salomon Dermatology 224, 84-92 (2012)
100 m
Corneocytes
Fingerprints (in-vivo)
Sweat duct
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- Cell count in native tissues and scaffolds
- Volume and shape analysis
CELL IMAGING
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Adipocytes in human tissue
9 m depth 27 m depth 60 m depth
Reconstructed
depth slice
200 m
50 m
200 m 200 m
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Effect of fibrosis on adipocyte shape
Reconstructed vertical views of adipocytes in gel 3D modelling of adipocyte
No fibrosis
With fibrosis
50 m
50 m
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Cell scaffold
Scaffold en-face view
Scaffold reconstructed depth slice
100 m
100 m
Courtesy of University of Toronto, Canada
Hepatocytes nuclei are visible
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Individual cells
100 m
Courtesy of Universit de Reims, France
Isolate collagen cells
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- Time series analysis
- Reconstructed depth slices and 3D imaging
DEVELOPMENTAL BIOLOGY
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In vivo imaging of drosophila melanogaster:
automated time series over 72 hours
Prepupa (0-2 h)Transition to
pupal stage (24 h)
Pupal stage (48 h)Advanced pupal phase
before eclosion (72 h)
Courtesy of ESPCI, Paris, France
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100 um
In vivo imaging of drosophila melanogaster
4 days
Courtesy of ESPCI, Paris, France
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Otic vesicle
Cartilage
Xenopus Laevis
100 um
Pigmented cells
Courtesy of Muse National dHistoire Naturelle, Paris, France
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Xenopus Laevis: back/tail
100 um
30 m depth 60 m depth
MyoseptumEpithelial
cells
Neural
cells
Courtesy of Muse National dHistoire Naturelle, Paris, France
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Xenopus Laevis
50 um
Reconstructed vertical slices from stacks of images
Courtesy of Muse National dHistoire Naturelle, Paris, France
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Xenopus Laevis eye: en face slices
Surface 10 m depth 20 m depth 30 m depth
40 m depth 50 m depth 60 m depth 70 m depth
80 m depth200 um
230 m depth140 m depth 180 m depth
Courtesy of Muse National dHistoire Naturelle, Paris, France
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Copepod
100 um
Courtesy of Laboratoire de Rgulation des Signaux de Division, EA4479, University of Lille
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Zebra fish eye
100 m
100 m
En face slices
Reconstructed depth slice
Courtesy of Inserm, Paris, France
10 m depth 20 m depth 45 m depth
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Zebrafish brain female, 1 year old
50 um
Courtesy of Laboratoire Hubert Curien, Saint Etienne
Reconstructed view from stack of images
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30 m depth
ovocytes
intestine
100 m
C. Elegans Fast identification of anatomy
Body wall - cuticle
gonad arm
uterus100 m
15 m depth
LLTech 2012 Courtesy of ENS, Paris, France
3D reconstruction
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- High resolution reconstructed depth slicesand 3D imaging
PLANTS
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Leaf veins fresh leaf sample
Fibers
Veins
100 m
500 m
Courtesy of ESPCI, Paris, France
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Apple
100 m
Wax
Skin
Flesh
500 m
Water
Courtesy of ESPCI, Paris, France
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Sorghum : cross section
Wide field en face
14 mm x 15 mm
Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging
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800 m x 800 m en face field
Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV)Montpellier RIO Imaging
Sorghum cross section: native field view
Reconstructed
depth slices
500 m sample
thickness
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Rice: hypocotyle
Wide field en face
5mm x 2mm
Reconstructed depth slice, 200um penetration depth
Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging
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Contacts
LLTech, Inc.
103 Carnegie Center Drive
Suite 300
Princeton, NJ 08540, USA
Phone: +1 609 995 3506
LLTech
Ppinire Paris Sant Cochin
29 rue du Faubourg Saint Jacques
75014 Paris
Phone: +33 1 82 72 61 25
www.lltechimaging.com