ASC Zerreciklerinden Yeni...
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ASC Zerreciklerinden Yeni Nesil
COVID 19 Aşı Teknolojisi
Nesrin Özören, Ph.D. Boğaziçi Üniversitesi
Moleküler Biyoloji ve Genetik Bölümü
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ASC Zerrecikleri Nasıl Oluşur
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PYD CARD
ASC Proteininin NMR Yapısı
De Alba ve ark., 2006
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ASC speck
ASC specks are produced in
HEK293T cells by
transfection of pEGFP-ASC
plasmid.
Blue: Nucleus
Red: Cytoplasm
ASC Zerreciklerinin Taşıyıcı Olarak Patentlenmesi
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wt L68A(PYD) R160A(CARD) L68A+R160A
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ASC Zerreciklerinin Taşıyıcı Olarak Patentlenmesi
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Sahillioglu A. C. & Ozoren N. 2015
Sahillioğlu A.C et. al, 2014
ASC
ASC Zerrecik Oluşum Mekanizması
(Hoss et al., 2016)
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ASC Zerrcikleri Canlıda- in vivo
Sagoo et. al. 2016
Nature Medicine
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ASC specks
produced in 293T
cells (red)
Macrophages
(green)
after endocytosis
of
ASC specks (red)
=
A B C
ASC Zerrecikleri Makrofajlar Tarafından Yutuluyor
ASC specks
extracted from
293T cells
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macrophages and
dendritic cells engulf and
degrade pathogens
T cell
macrophages and dendritic
cells process and display
antigens
macrophage
ASC Zerrecikleri Makrofajlar Tarafından Yutuluyor
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A B
C D
right after
purification
after 30 days
37°C
incubation
E F G
ASC Zerrecikleri Yüksek Isıda Dayanıklı
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Saflaştırılmış ASC Zerrecikleri
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Visualization of ASC specks (mcherry tagged) injected into mice i.p. injected i.v. injected
after 1 day
s.c. injected
untreated
-control
after 4hrs
50ug injection
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50ug mcherry-ASC vaccination is done i.v. and i.p.
i.v.
in
jectio
ns
i.p
. in
jectio
ns
At least stayed 2 weeks inside the body!
In both i.v. and i.p. ASC specks go through spleen, co-localized all together,
passed through lymph nodes in the gut.
After 3 weeks ASC specks disappear.
19.02.15 23.02.15 04.03.15
17.02.15 23.02.15 04.03.15
3. Stability of ASC specks inside mice body is identified (Preliminary study)
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Removed spleens are imaged via IVIS tech.
mcherry-ASC
injected mice
spleens
mcherry-
ASC+Freund’s
adj. injected
mice spleens
OVA-ASC
injected mice
spleens
PBS injected
mice spleens
After 2 weeks; ASC localize in the spleen!
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Saf tOVA-ASC ve mCherry-ASC Zerrecikleri
Purified mCherry-ASC specks
Purified tOVA-ASC specks
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Patentlerimiz
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Thank You Ali Can!
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19
Apoptosis and Cancer Immunology Laboratory
2007-2017
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Teşşekürler Lab üyelerimiz Aybüke Garipcan, M.Sc. Ceren Saygı, M.Sc. Açelya Yılmazer Mesut Berber Aylin Alkan Ozan Otaş Olay Gizem Artık
Eski lab üyelerimiz Serkan Uğurlu, M.D, Ph.D. Elif Eren, Ph.D.
Ali Can Sahillioğlu, M. Sc. Seda Yaşa, M. Sc. Yetiş Gültekin, M. Sc. Duygu Demiröz, M. Sc. Mustafa Yalçınkaya, M. Sc. Ulaş Özkurede, M. Sc. Metehan Çifdalöz, M. Sc. Burcu Sümer, M. Sc. Damla Erdoğan, M. Sc. Xara Xarsou, M. Sc.
Hulusi Onur Kuzucu, M. Sc.
Cihan Aydın, Ph.D.
Şahru Yüksel, Ph.D.
Ortaklar Türkan Haliloğlu, Ph. D. Fidan Sümbül, Ph. D. Özel Teşekkürler Stefan Fuss, Ph.D. Arzu Çelik, Ph.D. Batu Erman, Ph.D. Amitav Sanyal, Ph.D. İbrahim Taştekin, M.Sc. Xalid Bayramlı, Ph.D. Arzu Temizyürek, DVM İhsan Gürsel, Ph. D. Mayda Gürsel, Ph. D.
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22
COVID-19 Takımı