A novel strategy for rapid detection of bacteria in water by

the combination of three-dimensional surface-enhanced

Raman scattering (3D SERS) and laser induced breakdown

spectroscopy (LIBS)

Wenlong Liao, ‡a Qingyu Lin, ‡b Shicheng Xie, b Yi He, c Yonghui Tian,b Yixiang Duan a,b*

a School of Chemical Engineering, Sichuan University, Chengdu 610065, P.R.China

b Research Center of Analytical Instrumentation, Key Laboratory of Bio-resource and

Eco-environment, Ministry of Education, College of Life Sciences, Sichuan

University, Chengdu 610065, P.R.China

c Analytical & Testing Center, Sichuan University, Chengdu 610065, P.R.China

Fig. S1 The corresponding time evolution of Raman intensities of S.aureus (C) and E.coli

25922 (D) at 1356 and 1517 cm-1.

Fig.S2 The SERS spectra of four different bacterial species

Fig.S3 The SERS spectra of different concentrations of S.aureus.

Fig.S4 (A) Raman intensity of S.aureus at 1517 cm-1 vs. logarithm of the corresponding

concentration. (B) Linear fitting logarithm of Raman intensity of S.aureus at 1517 cm-1 vs.

concentration. Error bars represent the standard deviation of five independent measurements.

Fig. S5 (A) Photograph of the dried sample droplet after LIBS measurement, (B) SEM image of

the laser ablated crater in the dried sample droplet.

Fig. S6 Fitted peak areas vs. logarithm of the corresponding concentrations.