Application of EoNPV Against Tea Looper Ectopis

obliqua in China

Tea Research Institute Chinese Academy of Agricultural Sciences

Qiang Xiao26-10-2010

• One of the major pests of tea bushes in China• Occurs in most tea plantations• Zhejiang, Jiangsu, Hunan, Anhui Provinces

Ectropis obliqua Prout

The Life Cycle

• Feed on tea leaf

• Make tea bushes brown and bare

Anniversary Occurrence of Ectropis obliqua

0500

100015002000250030003500400045005000

3 4 5 6 7 8 9 10 11 12

month

amou

nt /m

u

• Main natural enemies ——parasitic wasp & parasitic fungi

• parasitic wasp • parasitic fungi

• One of the major natural enemies• Very effective in the field

Ectropis obliquaNucleopolyhedrovirus (EoNPV)

• Found in 1978

• Determined morphological and biological characters

• Analyzed gene sequence

• Researched to use in the field

EoNPV

Control effect of EoNPV against tea looper at different concentration

0

20

40

60

80

100

15 75 150 300

Usage doses (108PIB/ha)

Mor

talit

y (%

)15 days after spraying20 days after spraying

Control effect of EoNPV against the tea looper larvae of different instars

1 2 3 4

0

20

40

60

80

100M

orta

lity

(%)

Instars

Control effect of EoNPV against tea looper at different temperatures in the field

0

20

40

60

80

100

19 21 25 29

Average Temperature (℃)

Mor

talit

y (%

)

Morality of tea looper fed with two types of EoNPV formulation

0

20

40

60

80

100

4 6 8 10 12 14Days after feeding Eo NPV (d)

Mor

talit

y (%

)

EoNPVEoNPV-BT

• Established the better way of reproducing virus

• Found the commercial formulation

• Using methods in the field

• Be registered as bio-pesticide agent

Applications of EoNPV

0

500

1000

1500

2000

2500

3000

appl

ied

acre

age

(ha)

1990 1999 2001 2003 2005 2007 2009

year

Virulence of EoNPV in different geographic populations of tea looper

geographic populations

0

12

34

56

78

9

HangZhou

YiXing

QuZhou

YangZ

hou

WuHan

XiangFan

GaoQiao

700×

log

LC

50

conclusion

• EoNPV against tea looper is effective

• EoNPV as a microbial biocontrol agent has be registered

• EoNPV agent is be using to control tea looper rapidly

• Research should focus on the field application