APPLICATION NOTE PENTRA 400 1-ANTITRYPSIN SD (AUT-KIT)§ão-de-amostra.pdf · Factor 1 20.0 POST...

APTEC DIAGNOSTICS 23-04-2007

APPLICATION NOTE PENTRA 400

1-ANTITRYPSIN SD (AUT-KIT)

1. Reagent preparation Sample: Ready for use Reagent 1: Buffer, ready for use Reagent 2: Antiserum, ready for use Calibrator: Ready for use

2. Instrument setting

GENERAL PARAMETERS CALIBRATION PARAMETERS

TEST NAME AAT PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 20.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 20.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -

CORRELATION CALIBRATION

Slope 1.00000 Calibration Mode Logit/Log5

Intercept 0.00000 Level 6 Calibration Factor - Run (s) 2 VALIDITY On Request

ANALYSIS PARAMETERS CALCULATION PARAMETERS

ANALYSIS SEQUENCE CALCULATION TYPE End Point SAMPLE CYCLE 1 First Reading Cycle 8 VOLUME 6.0 µL Last Reading Cycle 40 DILUENT 1 NAME H2O VOLUME 10.0 µL

REAGENT R1 CYCLE 1 UNITS PARAMETERS

VOLUME 160 µL Unit mg/dL REAGENT R2 CYCLE 9 Conversion Factor 1.000000 VOLUME 20.0 µL DILUENT R2 NAME H2O VOLUME 10.0 µL WAVELENGTH Primary Wavelength 340 Secondary Wavelength - MIXING SPEED 40

CALIBRATION

TARGET VALUE ENTRY

Test AAT

Main Standard ** ** Standard value multiplied by dilution factor

3. Ordering information AAT/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



1- ACID GLYCOPROTEIN SD (AUT-KIT)




TEST NAME AGP PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test AGP


3. Ordering information AGP/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STL-001 Protein Standard Low, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



COMPLEMENT C3 SD (AUT-KIT)




TEST NAME C3C PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test C3C


3. Ordering information C3C/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



CERULOPLASMIN SD (AUT-KIT)




TEST NAME CER PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test CER


3. Ordering information CER/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



HAPTOGLOBIN SD (AUT-KIT)




TEST NAME HAP PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test HAP


3. Ordering information HAP/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL

APTEC DIAGNOSTICS 17.10.2008


IgA KIT 2

nd GENERATION




TEST NAME IgA2 PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 20.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 20.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgA2


3. Ordering information 104C002 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



IgG KIT 2nd

Generation




TEST NAME IgG PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 20.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 20.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgG


3. Ordering information 105C003 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High , 1 mL MPS/STS-5X1 Protein Standard Set, 5 x 1 mL

139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



IgM KIT 2

nd GENERATION




TEST NAME IgM2 PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 15.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 15.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgM2





PREALBUMIN SD (AUT-KIT)




TEST NAME PAL PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10.0 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test PAL


3. Ordering information PAL/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



TRF SD (SEL-KIT)




TEST NAME TRF PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 10.0 POST DILUTION 5.0 Factor 2 2.0 POST CONCENTRATION 2.0 Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name Saline Factor 6 0.0 Factor 10 Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test TRF


3. Ordering information TRF/SEL-000 1 x 5 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein standard high, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL

APTEC DIAGNOSTICS 23.04.2007 APPROVED APPLICATION


1-ANTITRYPSIN N-DIL (AUT-KIT)

1. Reagent preparation Sample: Ready for use. Reagent 1: Buffer, ready for use. Reagent 2: Antiserum, ready for use. Calibrator: Ready for use.



TEST NAME AAT PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 1.0 POST DILUTION 2.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 0.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test AAT

Main Standard ** ** Standard value

3. Ordering information AAT/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



1- ACID GLYCOPROTEIN N-DIL (AUT-KIT)

1. Reagent preparation Sample: Ready for use. Reagent 1: Buffer, ready for use. Reagent 2: Antiserum, ready for use. Calibrator: Ready for use.



TEST NAME AGP PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 0.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) - Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test AGP


3. Ordering information AGP/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



ASL(O) N-DIL (AUT-KIT) LATEX

1. Reagent preparation Sample: Ready for use Reagent 1: Buffer, ready for use Reagent 2: Latex, ready for use Calibrator: Ready for use



TEST NAME ASL PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 3.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -







VOLUME 140 µL Unit IU/mL REAGENT R2 CYCLE 7 Conversion Factor 1.000000 VOLUME 30.0 µL DILUENT R2 NAME H2O VOLUME 10.0 µL WAVELENGTH Primary Wavelength 600 Secondary Wavelength - MIXING SPEED 40

CALIBRATION

TARGET VALUE ENTRY

Test ASL


3. Ordering information ASL/AUT-000 1 x 10 mL Latex 5 x 25 mL Buffer ASL/STH-001 ASL Standard High, 1 mL ASL/CON-001 ASL Control, 1mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



COMPLEMENT C3 N-DIL (AUT-KIT)




TEST NAME C3C PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION 2.0 Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test C3C





COMPLEMENT C4 N-DIL (AUT-KIT)




TEST NAME C4C PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test C4C





CERULOPLASMIN N-DIL (AUT-KIT)




TEST NAME CER PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test CER


3. Ordering information CER/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



CRP N-DIL (AUT-KIT)




TEST NAME CRP PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test CRP


3. Ordering information CRP/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer CRP/STH-001 CRP Standard High, 1 mL CRP/COL-001 CRP Control Low, 1 mL CRP/COH-001 CRP Control High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



HAPTOGLOBIN N-DIL (AUT-KIT)




TEST NAME HAP PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test HAP


3. Ordering information HAP/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



IgA KIT 2

nd GENERATION




TEST NAME IgA2 PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 1.0 POST DILUTION 5.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 0.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgA2





IgG N-DIL (AUT-KIT)




TEST NAME IgG PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 5.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgG


3. Ordering information IGG/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



IgM KIT 2

nd GENERATION




TEST NAME IgM2 PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main Indirect NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 1.0 POST DILUTION 5.0 Factor 2 2.0 POST CONCENTRATION - Factor 3 2.0 Factor 4 2.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 0.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test IgM





MAL N-DIL (AUT-KIT) 2

nd generation

1. Reagent preparation Sample: Centrifuged urine, ready for use Reagent 1: Buffer, ready for use Reagent 2: Antiserum, ready for use Calibrator: Ready for use



TEST NAME MAL 2nd PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 3.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -







VOLUME 170 µL Unit mg/L REAGENT R2 CYCLE 10 Conversion Factor 1.000000 VOLUME 30.0 µL DILUENT R2 NAME H2O VOLUME 10.0 µL WAVELENGTH Primary Wavelength 340 Secondary Wavelength - MIXING SPEED 40

CALIBRATION

TARGET VALUE ENTRY

Test MAL 2nd


3. Ordering information 102C002 1 x 10 mL Antiserum 5 x 25 mL Buffer MAL/STD-001 Microalbumin Standard, 1 mL MAL/CON-001 Microalbumin Control, 1 mL MAL/CON-005 Microalbumin Control, 5 mL



PREALBUMIN N-DIL (AUT-KIT)




TEST NAME PAL PRE-DILUTION SAMPLE TYPE Serum/Plasma Type Main direct NUMBER OF REAGENTS Reagent 2 Calibrator Diluent Saline Factor 1 150.0 POST DILUTION 2.0 Factor 2 16.0 POST CONCENTRATION - Factor 3 8.0 Factor 4 4.0 PRE-DILUTION Factor 5 2.0 Diluent Name - Factor 6 1.0 Factor - Factor 7 - Incubation time (in cycles) 1 Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test PAL


3. Ordering information PAL/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL



TRANSFERRIN N-DIL (AUT-KIT)

1. Reagent preparation Sample: Ready for use Reagent 1: Buffer, ready for use Reagent 2: Antiserum, ready for use Calibrator: TRF standard set. Use saline 9g/L as zero point.



TEST NAME TRF PRE-DILUTION SAMPLE TYPE Serum/Plasma Type - NUMBER OF REAGENTS Reagent 2 Calibrator Diluent - Factor 1 - POST DILUTION - Factor 2 - POST CONCENTRATION - Factor 3 - Factor 4 - PRE-DILUTION Factor 5 - Diluent Name - Factor 6 - Factor - Factor 7 - Incubation time (in cycles) - Factor 8 -








CALIBRATION

TARGET VALUE ENTRY

Test TRF


3. Ordering information TRF/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer TRF/STS-5X1 Transferrin Standard Set, 5 x 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL

APPLICATION NOTE PENTRA 400 1-ANTITRYPSIN SD (AUT-KIT)§ão-de-amostra.pdf · Factor 1 20.0 POST...

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