Appendix I COMPOSITION OF MEDIA USED Composition of...
Transcript of Appendix I COMPOSITION OF MEDIA USED Composition of...
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Appendix I
COMPOSITION OF MEDIA USED
Composition of Nutrient broth
Ingredients g/L
Peptic digest of animal tissue 10.00
Sodium chloride 5.00
Beef extract 5.00
Distilled water 1 liter
pH 7.4 ± 0.2
Composition of Nutrient agar: 1.5 per cent agar added to Nutrient broth
Composition of Pikovskaya’s medium
Ingredients g/L
Glucose 10.0 g
Tricalcium phosphate 5.0 g
Ammonium sulphate 0.5 g
Potassium chloride 0.2 g
Magnesium sulphate 0.1 g
Manganese sulphate Trace
Ferrous sulphate Trace
Yeast extract 0.5 g
Agar 15.0 g
PH 7.0 ± 0.2
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Composition of King’s B agar
Ingredients g/L
Proteose peptone No.3 20.0
Dipotassium hydrogen phosphate 1.50
Magnesium sulfate heptahydrate 1.50
Agar 20
pH 7.2 + 0.2
Composition of Jensen’s medium
Ingredients g/L
Sucrose 20.0
Dipotassium hydrogen phosphate 1.0
Magnesium sulphate 0.5
Sodium chloride 0.5
Ferrous sulphate 0.1
Sodium molybdate 0.005
Calcium carbonate 2.0
Distilled water 1 litre
Composition of Jensen’s agar medium: 1.5 per cent agar added to Jensen’s medium
Composition of NBRIP broth
Ingredients g/L
Glucose 10
Tricalcium phosphate 5
Magnesium chloride hexahydrate 5
Magnesium sulfate heptahydrate 0.25
Potassium chloride 0.2
Ammonium sulfate 0.1
Double distilled water 1.0
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Composition of Peptone water
Ingredients g/L
Peptic digest of animal tissue 10.00
Sodium chloride 5.00
Distilled water 1 litre
pH 7.5 ± 0.2
Composition of Potato Dextrose Agar (PDA)
Ingredients g/L
Potatoes infusion 200.0
Dextrose 20.0
Agar 15.0
pH 5.6 ± 0.2
Composition of Trypticase soya broth
Ingredients g/L
Pancreatic digest of casein 17.0
Papaic digest of soyabean meal 3.0
Sodium chloride 5.0
Dipotassium hydrogen phosphate 2.5
Dextrose 2.5
pH 7.3 ± 0.2
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Composition of Succinate Broth
Ingredients g/L
Dipotassium Hydrogen orthophosphate 6.00
Potassium dihydrogen orthophosphate 3.00
Magnesium sulphate heptahydrate 0.2
Ammonium sulphate
Succinic acid
Distilled water
pH
1.0
4.0
1 litre
7.0
Composition of Trypticase Soya Agar
Ingredient g/L
Tryptone 17.00
Peptone 3.00
Sodium chloride 5.00
Glucose 2.50
Dipotassium phosphate
Agar
Distilled water
pH
2.50
20
1 litre
7.2±0.2
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Compostion of Anaerobic agar (Brewer)
Ingredient g/L
Proteose peptone 10.000
Casein enzymic hydrolysate 5.0
Yeast extract 5.0
Dextrose 10.0
Sodium chloride 5.0
Sodium thioglycollate 2.0
Sodium formaldehyde sulphoxylate 1.0
Resazurin 0.002
Agar 15.0
pH 7.2±0.2
Composition of Actinomycetes Isolation Agar
Ingredient g/L
Sodium caseinate 2.0
L-Asparagine 0.1
Sodium propionate 4.0
Dipotassium phosphate 0.5
Magnesium sulphate 0.1
Ferrous sulphate 0.001
Agar 15.0
pH 8.1±0.2
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Composition of Carboxy Methyl Cellulose (CMC) medium
Ingredient g/L
Carboxymethyl cellulose 2.0
Sodium nitrate 1.0
Dipotassium phosphate 1.0
Potassium chloride 1.0
Magnesium sulphate 0.5
Ferrous sulphate 0.01
Yeast extract 5.0
pH 7.2±0.2
Composition of Carboxy Methyl Cellulose (CMC) agar medium: 1.5 per cent agar
added to carboxymethyl cellulose (CMC) medium
Composition of Colloidal Chitin medium
Ingredient g/L
Colloidal chitin 5
Magnesium sulphate 0.5
Dipotassium hydrogen phosphate 0.7
Dihydrogen potassium phosphate 0.3
Ferrous sulphate 0.01
Mangenese chloride 0.001
Sodium chloride 0.3
Yeast extract 0.2
Agar 15.0
pH 7.0±0.2
Composition of Colloidal Chitin agar medium: 1.5 per cent agar added to colloidal
chitin medium
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Composition of Tributyrin agar medium
Ingredient g/L
Peptic digest of animal tissue 5.0
Yeast extract 3.0
Agar 15.0
pH 7.0±0.2
After dissolving the media in 990 mL of distilled water, 10 mL of tributyrin was added
Composition of Aleksandrov agar medium
Ingredient g/L
Glucose 10
Yeast extract 5
Magnesium sulphate 0.005
Ferric chloride 0.1
Calcium carbonate 0.1
Calcium phosphate 2
Potassium aluminium silicate 5
Agar 15.0
pH 7.0±0.2
All the above media were sterilized in autoclave at 121º C at 15lb/inch2 for 20 min
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Appendix II
Reagents
1.) Barton’s reagent
For the preparation of Barton’s reagent following two solutions were prepared:
I. Solution A: 25 g ammonium molybdate was dissolved in 400 mL ddw.
II. Solution B: 1.25 g ammonium metavanadate was dissolved in 300 Ml of boiling
water, cooled and then 250 mL concentrated HNO3 was added to it.
Then solution A and B were mixed and volume made upto one litre.
2.) Chrome Azurol Sulphonate (CAS) reagent
CAS assay solution:
a) Solution A (2 mM CAS stock solution): 0.121 g of CAS was dissolved in 100
mL distilled water.
b) Solution B (1 mM Fe stock solution): 1 mM solution of FeCl3.6H2O was made
in 10 mM HCl.
c) Solution C (Piperazine buffer): 4.307 g Piperazine was dissolved in 30 mL
distilled water. The pH of the solution was adjusted to 5.6 by adding concentrated
HCl to the solution
d) Solution D (HDTMA solution): 0.0219 g HDTMA was dissolved in 50mL
distilled water.
1.5 mL of solution B was mixed with 7.5 mL of solution A and this solution was added to
solution D in a measuring cylinder. Solution C was then added to it and the final volume
was made up to 100mL with distilled water.
Shuttle solution: 0.2 M 5-sulfosalicylic acid solution was prepared and stored in the
darkness.
3.) Salkowski reagent: 1 mL of 0.5 M FeCl3 dissolved in 49 mL of 35% perchloric
acid.
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4.) Nessler’s Reagent: 100 g Mercuric iodide and 70 g potassium iodide were
dissolved in a small quantity of distilled water and this mixture was added slowly,
with stirring, to a 500 mL cooled solution of sodium hyroxide (160 g sodium
hyroxide dissolved in 500 mL distilled water) and finally, this solution was diluted
to 1 litre with distilled water. Reagent was stored in rubber-stoppered borosilicate
glassware in dark to maintain its stability.
5.) DNS reagent: 2 g dinitrosalicyclic acid, 0.4 g phenol, 0.1 g sodium sulphite and 40
g Rochelle salt were dissolved in 100 mL of 2 % (w/v) NaOH solution and diluted
to 200 ml with distilled water
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Appendix III
16S rRNA gene sequences of potential bacterial isolates submitted to
GenBank, National Center for Biotechnology Information (NCBI), USA
>CS4B1(KJ78117)
CTGGCACCAAAAATGTGCGGCCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGG
TAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAAC
ATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGG
CGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGC
AGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGC
TCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACT
ACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTT
CTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGA
GTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAA
CTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTA
AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTG
AAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC
AGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCG
TCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGG
CACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGC
TACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTT
CGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTT
CCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACACCCTGAGCGAG
CCCGAGCCGGGGACCG
> CS4B2(KJ78118)
GACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACGGGGGCTAATACCGGATAACAT
TTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGG
TGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCC
CAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATG
AAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTA
ACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCG
TAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAG
ACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGA
AGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCA
CGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTG
GGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA
AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTG
ACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTT
AGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCAT
CATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAAT
CTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCA
GCATGCCGCGGTGAATACGTTCCCGGGCCCTTTGTTACCTAACT
> CS4B3(KJ78119)
ACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATT
TTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGT
GAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCC
AGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA
AGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAA
CCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGT
AAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGA
CTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAA
GGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCAC
GCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGG
GGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAA
GCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGA
CAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTA
GTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATC
ATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTAATC
TCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAG
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CATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGT
GGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATC
> CS4B4(KJ78120)
TGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGA
TGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATA
CCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCAT
TAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACT
GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCG
CGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTT
GACCGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGA
ATTATTGGGCGTAAAGGGCTCGCAGGCGGTTCCTTAAGTCTGATGTGAAAGCCCCCGGATCAACCGGGGAGGGTCATTG
GAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAAC
ACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACC
CTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAG
CACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTG
GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCG
GGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA
CCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGA
CGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCAAAACCGCGAG
GTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTA
ATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACA
CCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGT
AGCCGTATCGGAAGGTGCGGCTGGATCA
> CS4B5(KJ78121)
CAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAA
CTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACT
TACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAG
GGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACG
AAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTAC
CGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACG
TAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCG
GCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAA
ATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGG
GGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTA
GTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCC
CGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCC
TAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG
TTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACA
AACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAA
CAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTG
CGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGT
CACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAAATTATCTTCCAGG
> CS4B6(KJ78122)
GGGGCGTGCTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACG
TGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTC
AAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACC
AAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGG
CAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTA
AAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCT
AACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCG
GTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAG
GAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCT
GTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT
GCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAG
ACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCT
TACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTT
GTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGT
TGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCT
GGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTC
AGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATA
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CGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGG
AGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAA
> CS3B5(KJ78123)
CGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGGAGTAACACGTGGGTAACCTGCCTGTAAGA
CTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCG
GCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCG
ACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCG
CAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAG
AACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGC
GGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGA
AAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGT
AGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCG
AAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTC
CGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTG
ACGGGGGCCCGCACCAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTC
TGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGA
GATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTG
CCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAA
TGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCA
ACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACAC
ACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGG
> CS3B6(KJ78124)
GGGGGGGGGGCCGCCGTTCTATACTGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGT
GAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAAC
CGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTA
ACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTC
CTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTT
TCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAA
AGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGG
CTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAG
TGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGAC
TCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTA
AACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTA
CGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACG
CGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTG
GTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCC
AGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCC
CTTATACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAA
TCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCG
CGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAA
CCTTTTAGGAGCCAGCCGCTGAAAGGGTGACCCGGGGA
> CS2B6(KJ78125)
TAATTTGGGTATCTCCAGCGGACAGAAGGGCTGGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACC
TGCCTGTAAGACTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATGGTTCAAGGATGAA
AGACGGTTTCGGCTGTCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACG
ATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAG
GGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGT
TGTTAGGGAAGAACAAGTGCAAGAGTAACTGCTTGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGC
CAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAG
TCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAA
TTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACG
CTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT
AGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTC
AAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT
TGACATCCTCTGACAACCCTAGAGATAGGGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCT
CGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCT
AAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACA
CGTGCTACAATGGACAGAACAAAGGGCTGCGAGACCGCAAGGTTTAGCCAATCCCACAAATCTGTTCTCAGTTCGGATC
GCAGTCTGCAACTCGACTGCGTGAAGCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGC
CTTGTACACACCGCCCGTCACACCACGAGAGTTTTTCTATTTTATTTCCGT
189
189
> CS4A1(KJ78126)
GAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGG
AAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGAT
GGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATC
GGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCT
GACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGA
ATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGG
CAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCCGCTCAAC
CGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTA
GAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGA
ACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGC
AGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAA
GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGAT
AGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGA
GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGG
CAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAG
CTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA
CGAGAGTTTGTAACACCCGAAGTCGGT
> CS4A2(KJ78127)
TTATTGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTGAG
AGCTTGCTCTCAAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGG
AAACCGGGGCTAATACCGGATAAYATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGAT
GGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATC
GGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCT
GACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGA
ATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGG
CAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAAC
CGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTA
GAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAA
ACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGA
AGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAA
GCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGAT
AGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGA
GGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAG
CTGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGTAG
CTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA
CGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTG
AAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCT
> CS4A3(KJ78128)
CAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTGAGAGCTTGCTCTCAAGAAGTTAGCGG
CGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAAT
ATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTT
GGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGG
CCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGA
TGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACC
TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGG
CGTAAAGCGCGCGCAGGTGGTTTCTTAACTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGG
AGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGTAATGCGTAGAGATATGGAGGAACACCAGTGGC
GAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTC
CACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCC
TGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTC
GAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAG
TGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATC
TTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATC
ATCATGCCCCTTATGACCTGGGCTACATACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGAGGTGGAGCTA
ATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGAT
CAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTC
GGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATC
GGAAGGTGCGGCTGT
190
190
> CS4A4(KJ78129)
CAAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGGGGC
TAATACCGGATAACATTTTGAACTGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATGGACCCGCGT
CGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTG
GGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAA
CGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGC
ACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTAT
CCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGT
CATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGA
GGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAG
ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCA
TTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGC
ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTC
CTTCGGGAGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAG
CGCAACCCTTGATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGG
GATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACC
GCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGC
TAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTG
TAACACCCGAAGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAAC
AAGGTAGCCGTATC
191
191
Appendix IV
Table 1: Percent inhibition in the growth of F. oxysporum by different concentration of cell-free
extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of F. oxysporum by different
concentrations (v/v) of bacterial supernatants
100% 75% 50%
CS4B1 71 42 17.23
CS4B2 64.33 32.33 12.23
CS4B3 54.2 31.67 12
CS4B4 51.1 24.22 0
CS4B5 32.33 0 0
CS4B6 43.67 13.33 0
CS3B5 69 27.67 9.56
CS3B6 20.67 0 0
MTCC 7612 69.22 35 13
Table 2: Percent inhibition in the growth of S.rolfsii by different concentrations of cell-free
extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of S.rolfsii by different
concentrations of bacterial supernatants
100% 75% 50%
CS4B1 70.67 54.23 20.67
CS4B2 59.33 37.22 19.87
CS4B3 62.41 25.23 9
CS4B4 33.74 15.6 0
CS4B5 38.67 12.3 0
CS4B6 28.11 11 0
CS3B5 53.22 25.67 11
CS3B6 25.67 7.33 0
MTCC 7612 64 44 12
Table 3: Percent inhibition in the growth of P.aphanidermatum by different concentrations of cell-free extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of P.aphanidermatum by different
concentrations of bacterial supernatants
100% 75% 50% 25%
CS4B1 70.67 54.23 20.67 80.23
CS4B2 59.33 37.22 19.87 83.67
CS4B3 62.41 25.23 9 82.29
CS4B4 33.74 15.6 0 68.67
CS4B5 38.67 12.3 0 62.21
CS4B6 28.11 11 0 65.04
CS3B5 53.22 25.67 11 80.22
CS3B6 25.67 7.33 0 68.66
MTCC 7612 64 44 12 81
192
192
Table 4: Percent inhibition in the growth of C.truncatum by different concentrations of cell-free
extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of C.truncatum by different
concentrations of bacterial supernatants
100% 75% 50%
CS4B1 71.3 43.2 16.62
CS4B2 68.33 28.65 6.72
CS4B3 69.67 30.33 0
CS4B4 54.33 26 0
CS4B5 24.11 0 0
CS4B6 30.26 17 0
CS3B5 63.57 38.67 11.67
CS3B6 46.33 15 0
MTCC 7612 67 37 9
Table 5: Percent inhibition in the growth of R.solani by different concentrations of cell-free extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of R.solani by different
concentrations of bacterial supernatants
100% 75%
CS4B1 50.23 24.23
CS4B2 40.35 16.67
CS4B3 38.62 12.67
CS4B4 38.66 10.33
CS4B5 29.12 0
CS4B6 26 0
CS3B5 47.33 20.22
CS3B6 21.67 0
MTCC 7612 41 14
Table 6: Percent inhibition in the growth of A.solani by different concentrations of cell-free
extracts of bacterial supernatants
Isolates Per cent inhibition in the growth of A.solani by different
concentrations of bacterial supernatants
100% 75%
CS4B1 39.33 16.33
CS4B2 29.67 13.23
CS4B3 20.33 0
CS4B4 24.67 0
CS4B5 11.33 0
CS4B6 18.33 0
CS3B5 31.23 11.67
CS3B6 6 0
MTCC 7612 30 0
193
193
Table 7: Percent inhibition by volatile metabolites in the growth of F. oxysporum at different
stages of bacterial growth
Isolates Percent inhibition in the growth of F. oxysporum at different stages of
bacterial growth
0h 24h 48h 72h
CS4B1 56.52 68.37 65.78 30.96
CS4B2 46.52 64.3 57.63 15.78
CS4B3 48.74 63.19 54.3 13.34
CS4B4 42.81 61.33 54.63 26.33
CS4B5 46.52 55.41 51.7 0
CS4B6 52.07 64.67 58 0
CS3B5 50.59 65.41 59.11 15.41
CS3B6 31.33 47.63 34.59 0
MTCC 7612 48 66 63 27
Table 8: Percent inhibition by volatile metabolites in the growth of S.rolfsii at different stages of
bacterial growth
Isolates Percent inhibition in the growth of S.rolfsii at different stages of bacterial
growth
0h 24h 48h 72h
CS4B1 51.85 68.15 61.11 43.7
CS4B2 47.78 62.96 54.81 30.37
CS4B3 49.26 58.15 55.56 21
CS4B4 44.81 56.3 55.19 11.45
CS4B5 37.41 50 37.78 0
CS4B6 32.96 55.19 40.37 20.65
CS3B5 51.11 61.85 59.63 23.7
CS3B6 8.15 33.33 21.48 0
MTCC 7612 47 60 51 16
Table 9: Percent inhibition by volatile metabolites in the growth of P.aphanidermatum at different stages of bacterial growth
Isolates Percent inhibition the growth of P.aphanidermatum at different stages of
bacterial growth
0h 24h 48h 72h
CS4B1 70.96 73.7 72.59 44.44
CS4B2 67.41 70.37 67.04 31.85
CS4B3 54.44 67.41 62.22 37.04
CS4B4 57.41 71.85 71.85 26.67
CS4B5 55.56 70.74 71.48 36.3
CS4B6 61.48 71.85 71.11 38.89
CS3B5 54.07 68.15 67.04 47.41
CS3B6 51.48 65.93 63.33 18.65
MTCC 7612 68 71 65 31
194
194
Table 10: Percent inhibition by volatile metabolites in the growth of C.truncatum at different
stages of bacterial growth
Isolates Percent inhibition in the growth of C.truncatum at different stages of
bacterial growth
0h 24h 48h 72h
CS4B1 43.75 68.33 55 25
CS4B2 43.75 62.08 50 19.67
CS4B3 43.75 63.33 44.17 10.33
CS4B4 36.25 58.75 41.25 16.56
CS4B5 30.83 55 37.08 0
CS4B6 45.42 64.17 50 0
CS3B5 46.67 61.25 52.92 17.08
CS3B6 14.58 37.08 20.83 0
MTCC 7612 45 65 41 14
Table 11: Percent inhibition by volatile metabolites in the growth of R. solani at different stages
of bacterial growth
Isolates Percent inhibition in the growth of R. solani at different stages of
bacterial growth
0h 24h 48h 72h
CS4B1 43.7 58.15 48.89 23.35
CS4B2 37.78 54.81 46.67 21.67
CS4B3 39.63 57.41 50.74 25.74
CS4B4 42.59 48.89 45.93 0
CS4B5 32.22 45.93 37.78 0
CS4B6 28.89 52.96 41.11 0
CS3B5 46.3 56.67 51.85 0
CS3B6 15.56 34.44 22.59 0
MTCC 7612 40 54 42 17
Table 12: Percent inhibition by volatile metabolites in the growth of A. solani at different stages
of bacterial growth
Isolates Percent inhibition in the growth of A. solani at different stages of
bacterial growth
0h 24h 48h 72h
CS4B1 28.63 39.61 33.33 0
CS4B2 22.35 35.69 30.2 0
CS4B3 28.24 35.69 32.16 0
CS4B4 21.96 27.45 20.78 0
CS4B5 18.82 34.12 18.43 0
CS4B6 26.27 36.86 31.37 0
CS3B5 25.88 40.78 35.29 0
CS3B6 5 13.73 1.57 0
MTCC 7612 11 31 28 0