A.O.T.F Laser control
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Transcript of A.O.T.F Laser control
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A.O.T.F Laser control
• Thermally controlled Tellerium Dioxide (TeO2) crystal
Selected Wavelength
Kr/Ar Laser Light
Transducer
Non DefractedWavelengths
Acoustic Termination
BeamDump
Acousto-Optic-Tunable-Filter Principle
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Specifications
• Polarisation preserving >70:1 extinction
• > 85% transmision efficiency
• blocking > OD 4.0
• Noise = < 5.0% peak RMS (measured in reflection imaging mode as per std. commissioning spec.)
• switching frequency - how quick can you click a mouse button!
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AOTF DELIVERS High quality simultaneous multi label imaging
•A comparison of classic Neutral Density (ND) attenuation versus AOTF laser control
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Spectral separation considerations
AOTF allows more control of the laser intensities to allowbetter assessment of dye co-localisation
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FITC channel (522/32) using ND filter
CY3 channel (HQ598/40) using ND filter
CY5 channel (680/32) using ND filter
The FITC channel is bleeding into the CY3 channel in this set-up
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24 Bit merge of FITC/CY3/CY5 using ND filter
Cross talk between red and green channels
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Fluorogram of FITC & CY3 channels showing FITC bleed through (cross talk)
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FITC channel (522/32) using AOTF control
CY3 channel (HQ598/40) using AOTF control
CY5 channel (680/32) using AOTF control
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24 Bit merge of FITC/CY3/CY5 using AOTF control
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Fluorogram of FITC & CY3 channels with AOTF control - displaying little co-localisation
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The Comparison Again
AOTF imaging ND filter imaging
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