SEMINAR ON ANTIOXIDANT ACTIVITY OF INDIAN PROPOLIS

AND ITS CHEMICAL CONSTITUENTS

By

Abu SayeedCHM11021

DEPT OF CHEMISTRY ALIAH UNIVERSITY

INDEX

What is the aim of my project ? What is Antioxidant? What is Indian propolis? History of indian propolis ? Source of propolis in different geogrhaphical region in india ? Characteristics of propolis ? What the chemical constituents of Indian propolis? Structure of Active constituents of indian propolis? Difference between two active component of indian propolis? What is the advantages of Indian propolis? What is Use of Indian propolis?

WHAT IS THE AIM OF MY PROJET : Study Antioxidant activity of indian propolis and

its chemical constituent and pharmacological effect .

WHAT IS ANTIOXIDANT? An antioxidant is a molecule that inhibit oxidation of

other molecules. Oxidation is a chemical reaction involve loss of

electrons or increase oxidation state . In cellular system oxidation is a natural process. free radicals are generated which prompts chain

reactions. chain reactions cause cellular damage. eventually cause cell death or diseases .

WHAT IS ANTIOXIDANT?. Antioxidant terminated these chain reaction by

involving free radical intermediate , inhibit oxidation .

WHAT IS INDIAN PROPOLIS?

Propolis is a natural resinous mixture produced by honeybees from substances collected from parts of plants. Propolis, also known as ‘bee glue’, is the most important and interesting ‘chemical weapon’ of honey bees.

Fig:indian propolis

WHAT IS INDIAN PROPOLIS?

Propolis is having a composition more than 300 phytocompounds . Etymologically the word propolis derived from the Greek pro (for ‘in front of’, ‘at the entrance to’) and polis (for ‘community’ or ‘city’), meaning that this natural product is used for hive defense. Another name of propolis is bee glue. Nowadays, propolis is a natural remedy found in many health food stores in different forms for topical use.

HISTORY OF INDIAN PROPOLISPropolis has a long history of medicinal use, many

years  back to 350 B.C., the time of Aristotle. Greeks have used propolis for ulcer disease ; Assyrians have used it for healing wounds and tumors; and Egyptians have used it for mummification. It still has many medicinal uses today, although its effectiveness has only been shown for a couple of them.

PROPOLIS IN THE HIVE

(Kulincevica & Gacica, 1991)

DIFFERENT GEOGRHAPHICAL REGION

Propolis composition varies according to different geographical region. Difference shows due to

climate and temperature variati in india.

TABLE 2: GEOGRAPHIC(INDIAN ) ORIGIN AND ACTIVITY AND SOLVENT IN EXTRACTION METHOD :SL NO Geographic Region Activity Solvent used in

extraction1 Karnataka Antibacterial Petroleum ether,

chloroform, ethanol, methanol and 40% methanol

2 West Bengal Anti-oxidant Ethanol and water

3 Gujarat Antioxidant, antimicrobial.

Ethanol, water, petroleum ether, chloroform

4 Madhya Pradesh Anti-microbial, Ethanol

5 Maharashtra Anti-microbialanti-bacterial

Ethanol, Mthanol

SOURCE OF INDIAN PROPOLIS STATE WISE .

souce of indian propolis

Madhya PradeshwestbengalGujaratKarnataka

CHARACTERISTICS OF PROPOLIS Propolis is lipophilic in nature, Hard and brittle material when cold. when temperature rises it becomes soft. It is flexible, gummy and very sticky. It possesses a characteristic and pleasant aromatic

smell and varies in color from yellow green, to red ,black and to dark brown depending on its source .

MELTING POINT :

At temperatures of 25°C to 45°C propolis is a soft, flexible and very sticky substance. Particularly when frozen or at near freezing, it becomes hard and brittle. It will remain brittle after such treatmengt even at higher temperatures. Above 45°C it will become increasingly sticky and gummy. Propolis will become liquid at 60°C to 70°C, but for some samples the melting point may be as high as 100°C.

SOLUBILITY OF PROPOLIS

The most common solvents used for commercial extraction are as follows water, methanol, ethanol, chloroform, dichloromethane, ether, and acetone. Many of the bactericidal components are soluble in water or alcohol. These solvents remove the inert material and preserve the desired compounds.

CHEMICAL CONSTITUENTS OF INDIAN PROPOLIS

INDIAN PROPOLIS

galanginpinocembrin

STRUCTURE OF PINOCEMBRIN AND GALANGIN

FIG 1 : pinocembrin Fig 2 galangin

DIFFERENCE BETWEEN PINOCEMBRIN AND GALANGIN

1) Chemical formula :C15H12O42) CHEMICAL NAME :5,7

DIHYDROXYFLAVANON 3) MOLAR

MASS:256.25GM/MOL4) DENSITY :1.386 gm /ml

1) CHEMICAL FORMULA:C15H10O5

2) CHEMICAL NAME :3,5,7 TRIHYDROXY FLAVANONE

3) MOLAR MASS:272.25gm/mol4) DENSITY :1.597 gm/ml

ADVANTAGES OF INDIAN PROPOLIS: Over the last few decades, worldwide increase in

the use of natural products for pharmacological purposes has been observed.

1) Nowadays, propolis is a natural remedy found in many health food stores in different forms for topical use.

2)It is also used in cosmetics or as popular alternative medicine for self-treatment of various diseases.

3) Current applications of propolis include formulations for cold syndrome.

4) As well as dermatological preparations useful in wound healing, treatment of burns neurodermatitis.

5) Additionally, propolis is used in mouthwashes and toothpastes.

6) It is widely used in cosmetics and in health foods and beverages.

7) It is commercially available in the form of capsules (either pure or in extracts), mouthwash solutions (combined with lemon balm, sage, mallow), creams, throat lozenges, powder and also in many purified products from which the wax was removed .

8) it is widely used in human and veterinary medicine, pharmacology and cosmetics.

USE OF INDIAN PROPOLIS Propolis has been used in traditional medicines for

thousands of years.

Antimicrobial: Preliminary scientific studies show some types of

propolis have in vitro antimicrobial activity with active constituents including flavonoid like galangin and hydrocinamic acids like caffic acid. 

ANTI-VIRAL ACTIVITY Viral entry of HIV-1 was inhibited in CD4 lymphocytes. Effectiveness of the reverse transcriptase inhibitor,

zidovudine, was increased.

PROPOLIS DECREASED VIRAL EXPRESSION IN CD4 CELLS

(Gekker, Hu, Spivak, Lokensgard, & Peterson, 2005)

USE : IT has anti cancer activity It has antifungal activity . It is also used in cosmetics. Current applications of propolis include

formulations for cold syndrome.

USE: In food:.. Propolis is used by some chewing gum

manufacturers to make propolis gum.

Car wax: Propolis is used to bring about a chemical reaction

to convert fats and oils into automobile wax during application.

ACKNOWLEDGEMENT

DONE BY :Rajibul Arif Laskar, Ismail Sk, Nayan Roy, Naznin

Ara Begum, Bio-Organic Chemistry Lab, Department of

Chemistry, Visva-Bharati University, Santiniketan 731 235, India.

Supervisor Dr. H. Mir Head, Dept. of Chemistry All the faculty, Dept. of Chemistry,Aliah

university . My class mates…

THANK YOU FOR YOUR KIND ATTENTION