Antimicrobial activity of Ficus
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International Research Journal of Plant Science (ISSN: 2141-5447) Vol. 2(11) pp. 332-337, November 2011Available online http://www.interesjournals.org/IRJPSCopyright © 2011 International Research Journals
Full length Research Paper
Antimicrobial activity nutritional profile and quantitativestudy of different fractions of ficus palmata
Sarla Saklani1 and Subhash Chandra1*
1Department of Pharmaceutical Science, H. N. B. G. U. (Central University), Srinagar Garhwal Uttarakhand India
Accepted 30 August, 2011
The in vitro antibacterial and antifungal activities of petroleum ether, chloroform, ethyl acetate, acetone,methanolic, ethanolic and water extracts of Ficus palmata were tested against ten bacterial strains andthree fungal strains by disc diffusion method. The ethanolic bark extracts of Ficus palmata showedsignificant activity (18 mm) against Staphylococcus aureus. The medicinal plant fruit contain ash value,
(total ash) moisture; crude fat and crude fiber, extractive values were studied fresh part weight. Thepreliminary phytochemical analysis test showed the presence of carbohydrates and glycosides,alkaloid, flavonoids, saponins, tannins, unsaturated triterpenoids and sterol, resin.
Keywords: Antibacterial, antifungal, nutritional value, and phytochemical screening.
INTRODUCTION
Herbal medicine is the oldest form of health care knownto mankind. Herbs have been used by all culturesthroughout the history and they constitute an integral partof the development of modern civilization. Medicinal and
aromatic plants and their derived are rich in antibacterialcompounds which could be an alternate way to combatbacterial diseases even against some bacteria which arebecoming resistant to certain synthetic medicines(Ahmad et al., 1998; Aswal et al., 1996). In recent years,multiple drug/chemical resistance in both human andplant pathogenic microorganisms have been developeddue to indiscriminate use of commercial antimicrobialdrugs/chemical commonly used in the treatment ofinfectious diseases. This situation has forced scientists tosearch new antimicrobial substances in various sources(Kumar et al., 2006). The present study aimed atevaluating the antimicrobial activity of plant Ficus palmata
extracts against Gram-positive and Gram-negativebacterial strains isolated from human infections. Furtheracquaintance with different ethnic groups has
*Corresponding Author E-mail: [email protected]
ABBREVIATIONS:
MIC: Minimum Inhibitory Concentration.
contributed to the development of research on naturaproducts, to the increase in knowledge about the closerelationship between the antimicrobial activity of a certaincompound and its biological properties, In terms of using
plant materials for traditional medicinal plants, which formthe backbone of traditional medicine, have in the last fewdecades been the subject for very intensepharmacological studies (Anonymous et al., 1994; Aroraet al., 1997). For these reasons, medicinal plants areimportant substances for the study of their traditionauses through the verification of pharmacological effectsand can be natural composite sources that act as newanti-infectious agents.
MATERIAL AND METHODS
Plant Material
The fresh parts of fruit, bark, root and leaf of Ficuspalmata, was collected from adjoining area of Ghat city(Dist- Chamoli, Uttarakhand) in the month of August. Theplant was authenticated by botanist Dr. R. D. GuarDepartment of Botany; H. N. B. G. U. Srinagar Garhwal.
Preparation of plant Extract
The plant material was separated into its selected parts(bark, leaf, root and fruit) air dried ground to moderately
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fine powder and Soxhlet extracted with increasing polaritysolvent (Petroleum ether, chloroform, ethyl acetate,acetone, methanolic, ethanolic and water) (LIN et al.,1999). Each extract was evaporated to dryness underreduce pressure using rotary evaporator. The coarsepowder of fruit bark and root was subjected to successivehot continuous extraction with various solvent each time
before extracting with next solvent the powdered materialwill be air dried (weight of crude extract 100gm). Thevarious concentrated extracts were stored in air tightcontainer for further studies.
Media
Nutrient broth, Nutrient agar, Muller Hinton agar, Maltextract broth and Sabouraud dextrose agar, Alcohol,Hydrochloric acid, alcohol, and sulphuric acid, Distilledwater etc all product of Himedia Laboratories Mumbai(India) were used in this study.
Bacterial Strains
Ten bacterial strains were used namely Escherichia coli,Klebsiella pneumoniae, Enterobacter gergoviae,salmonella entericatyphim, shigella flexneri, Staphyloccusaureus, staphyloccus epidermidis, streptococcuspyogenes, and Bacillus cereus, The bacterial strainswere supplied by the Microbial Type Culture Collectionand Gene Bank, Institute of Microbial Technology,Chandigarh, India. (Customer no. 3921)
Fungal Strains
Three fungal strains were used namely Candida albicans,Aspergillus flavus and Aspergillus parasiticus, Thefungal strains were supplied by the Microbial TypeCulture Collection and Gene Bank, Institute of MicrobialTechnology, Chandigarh, India.
Antibacterial assay
The disc diffusion assay methods were used to determinethe growth inhibition of bacteria by plant extracts(Iennette et al., 1985, Rosoanaivo and Ratsimanaga1993). Diluted bacterial culture (100µl) was spread overnutrient agar plates with a sterile glass L-rod. 10mg/ml
and 50mg/ml of the each extracts were applied to eachfilter paper disc (Whatman No. 1, 5 mm diam.) andallowed to dry before being placed on the agar plate.Each extract was tested in triplicate (3 discs/ plate) andthe plates were inoculated at 37°C for 24 h. Afterincubation, the diameter of inhibition zones wasmeasured with a caliper.
Antifungal assay
The antifungal activity was tested by disc diffusion
Saklani and Chandra 333
method (Taylo et al., 1995; Espinel Ingroff, 2002). TheSabouraud dextrose agar plates were each similarlyseeded with each fungal strain The 24 hrs. broth cultureof each bacterium and 7 days inoculated fungus culturewere used to seed sterile Sabouraud dextrose agar a45°C respectively, and fungal plates were incubated at25-28°C for 7 days after which diameter of zones of
inhibition were measured. Each disc filled with extract.
Nutritional and Mineral assay
The number of water molecule is contain % of moisturePt. ether and hexane soluble part is called crude fat andthe non soluble part of acid- base medium is called crudefibre (cellulose and lignin), and mineral estimated byflame photometry (Gaur et al 1999, and Badoni 1994).
RESULT AND DISCUSSION
Plants are important source of potentially usefu
structures for the development of new chemotherapeuticagents. The first step towards this goal is the in vitroantimicrobial activity assay (Tona et al., 1998). Theresults of antibacterial, antifungal, nutritional value andphytochemical screening activity, table 1, 2, 3, and 4reveals that antibacterial, antifungal, nutritional, andphytochemical screening activity of bark and fruiexplants of Ficus palmata was evaluated against tenbacterial and three fungal pathogenic strains.
CONCLUSION
In conclusion, the results of this investigation revealedthat antimicrobial and antifungal activity against selectedbacterial and fungal strains. The differentiating activitiesagainst variety of microorganisms of these five fractionencourage developing a novel broad spectrumantimicrobial formulation in future. Now our research wilbe directed to develop a broad spectrum antimicrobiaherbal formulation with this plant. Even at lowconcentrations, these species showed high antimicrobiaand antifungal activity nearly equal to that of thecommercial fungicide used as a positive control. Furthestudies are needed to determine the chemical identity ofthe bioactive compounds responsible for the observedantimicrobial and antifungal activity. Natural plant-derived
fungicides may be a source of new alternative activecompounds, they can be used in the treatment oinfectious diseases caused by resistant microbes.
ACKNOWLEDGMENTS
The authors are grateful to the UCOST, Dehradun fofinancial support and the institute of microbial technologysector 39-A, Chandigarh India, for providing bacterial andfungal chemicals.
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334 Int. Res. J. Plant Sci.
Table1. Antibacterial activity of ten bacterial strains against Ficus palmata plant extract. Disc size, 5 mm, Inhibitory zone size±1 mm, mm means (millimetres) and – indicate (NIZ) No inhibitory zone.
Bacterial Name
Petroleumether Extract
Chloroform
Extract
Ethyl acetate
Extract
Acetone
Extract
Methanol
Extract
Ethanol
Extract
Water
Extract
Genus/Species/Subspe.
MTCC
(Code)
10
Mg/
ml
50
Mg/
ml
10
Mg
/ml
50
Mg
/ml
10
Mg/
Ml
50
Mg/
ml
10
Mg/
ml
50
Mg/ml
10
Mg/ml
50
Mg/ml
10
Mg/ml
50
Mg/ml
10
Mg/ml
50
Mg/ml
Bacillus cereus 1272 - - - 9 - 11 - - - 11 6 16 - 9
Escherichia coli 729 - - - 8 - 11 - 8 - 11 7 10 - 9
Enterobactergergoviae
621 - - - 7 - 14 7 - - 12 8 13 - 8
Klebsiellapneumonia
432 - - - 8 - 14 - - 9 11 6 12 - 7
Salmonellaentericatyphim
98 - - - 8 - 11 - 7 - 12 8 14 - 9
Shigella flexneri 1457 - 7 - 10 - 11 - 7 - 12 7 13 - 8
Staphyloccusaureus
902 - - 7 9 6 16 - - - 17 8 18 7 9
Staphyloccusepidermidis
435 - - 7 9 - 14 - - - 16 7 11 - 7
Streptococcuspyogenes
1925 - - 8 8 - 11 7 - - 14 6 12 - 8
Escherichia coli 443 - - 10 9 - 13 - - - 14 6 15 - 9
Table 2. Fungal activity of three fungal strains against Ficus palmata plant extract. Disc size, 5 Mm, Inhibitory zonesize ±1 Mm, Mm means (millimetres) and – indicate (NIZ) No inhibitory zone.
Fungal Name
PetroleumetherExtract
Chloroform
Extract
Ethylacetate
Extract
Acetone
Extract
Methanol
Extract
Ethanol
Extract
Water
Extract
Genus/Species/Subspe.
MTCC
(Code)
10
Mg/
ml
50
Mg/ml
10
Mg
/ml
50
Mg
/ml
10
Mg/
ml
50
Mg/
Ml
10
Mg/ml
50
Mg/
ml
10
Mg/
ml
50
Mg/ml
10
Mg/ml
50
Mg/
ml
10
Mg/ml
50
Mg/ml
Candidaalbicans
3017 - - - - - - - - - 8 - 8 - -
Aspergillusflavus
2798 - - - - - - - - - 7 - 7 - -
Aspergillusparasiticus
2796 - - - - - - - - - 7 - 8 - -
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Saklani and Chandra 335
Table 3. Nutritional value of Ficus palmata fruit.
Nutrients Value
Moisture (%) 48.20 ± 0.15
Ash (%) 4.06 ± 0.08
Total nitrogen (%) 0.73 ± 0.07
Total protein (%) 4.06 ± 0.04
Crude fat (%) 4.71 ± 0.25
Crude fibre (%) 17.65 ± 0.09
Carbohydrate 20.78± 0.16
Organic matter 95.90± 0.22
Ascorbic acid 0.83± 0.15
Energy value K Cal 107.37± 0.15
N (Mg/100gm) 0.73± 0.12
Ca (Mg/100gm) 1.54 ± 0.13
Mg (Mg/100gm) 0.92± 0.15
K (Mg/100gm) 1.58± 0.25
P (Mg/100gm) 1.88 ± 0.20
Fe (Mg/100gm) 0.018 ± 0.02
Table 4. Phytochemical screening of wild edible fruits F. P – Ficus palmata, (F – Fruit, B – Bark, R – Root,) (+) – Present, (-) – Absent,
Test FPF FPB FPRCarbohydrates/ glycosides
Molish test
Fehling test
Benedict test
(+)(+)(+)
(+)(+)(+)
(+)(+)(+)
Alkaloid
Mayer’s test
Dragondroff test
(-)
(+)
(+)(+)
(-)
(-)
Flavonoids (+) (+) (-)
Saponins (-) (+) (-)
Tannins
Pyrogoll & catechol
Gallic acid
(-)
(-)
(+)
(+)
(+)(+)
Unsaturated sterol/triterpenes
Liebermann Burchard test
Salkowiskis test
(+)(-)
(+)(+)
(+)(+)
Resin (-) (+) (+)
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336 Int. Res. J. Plant Sci.
Figure 1 and 2. Antimicrobial activity of ten bacterial strains and three fungal strainsAgainst Ficus palmata plant extract
Figure 3. Comparison of per day intake of nutrients by Adults with the nutrients present inthe fruits of Ficus palmata.
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Saklani and Chandra 337
Figure 4. Comparison of per day intake of minerals by Adults with the mineral present in thefruits of Ficus palmata.
REFERENCES
Ahmad I, Mehmood Z, Mehmood I (1998). Screening of some Indianmedicinal plants for their antimicrobial properties. J. Ethnopharmacol.62: 183- 193.
Anonymous (1994). Ethno-botany in India- A status report. All Indiacoordinated research project in Ethno -botany, Ministry ofEnvironment and Forests, Govt. of India, New Delhi.
Arora RK (1997). Ethno- botany and its role in the conservation and
use of plant resources in India. Ethno- botany, 9: 6-15.Aswal BS, Goel AK, Patneik GK (1996). Screening of Indian medicinal
plants for biological activity. Indian J. Exp. Biol., 34: 444- 467.Kumar RS, Sivakumar T, Sundaram RS, Sivakumar P, Nethaji R, Gupta
M, Mazumdar UK (2006). Antimicrobial and Antioxidant Activities ofCareya arborea Roxb. Stem Bark. Iranian J. pharmacol. Therapeut.5:35-41.
Lin J, Opak War, Geheeb-Keller M (1999). Preliminary screening ofsome traditional Zulu medicinal plants for anti-inflammatory andantimicrobial activities. J Ethnopharmacol. 68: 267–274.
Iennette EH (1985). Manual of clinical microbiology, 978–987. 4thedition. American Association for Microbiology, Washington.
Rosoanaivo , Ratsimanaga U (1993). Biological evaluation of plantswith reference to the Malagasy flora. Monograph for the IFsNAPRECA Workshop on Bioassays.
Gaur RD (1999). Flora of the district Garhwal North West Himalaya, 1sEd. Transmedia Srinagar Garhwal, July-1999,
Badoni S, Rawat MSM, Negi YS (1994). Nutritional Composition osome Berberis species, J. Ind. J. of Hort. Pub. Provide page
Tona L, Kambu K, Ngimbi N, Cimanga K, Vlietinck AJ (1998)Antiamoebic and phytochemical screening of some Congolesemedicinal plants. J. Ethnopharmacol. 61: 57- 65.
Taylor RSL, Manandhar NP, Hudson JB, Towers GHN (1995)Screening of selected medicinal plants of Nepal for antimicrobiaactivities. J. Ethnopharmacol., 546: 153-159.
Espinel Ingroff A, Fothergill A, Peter J, Rinaldi MG, Walsh TJ (2002)Testing conditions for determination of minimum fungicidaconcentrations of new and established antifungal agents foAspergillus spp.: NCCLS Collaborative Study. J. Clin. Microbiol40:3204-3208.