Rational HIV Immunogen Design to Target Specific Germline ...
Antigens/Antibodies · 2019. 10. 25. · Antigen vs. Immunogen •Antigen – Any substance that...
Transcript of Antigens/Antibodies · 2019. 10. 25. · Antigen vs. Immunogen •Antigen – Any substance that...
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Antigens/Antibodies
Chapters 4-6
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Antigen vs. Immunogen • Antigen
– Any substance that can bind to an antibody or T cell receptor
• Immunogen – Any substance that
can elicit an immune response
– All immunogens are antigens
– Not all antigens are immunogens, i.e. haptens
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Immunogenicity of Antigens • Determined by
– Foreignness – Molecular Size – Chemical
Composition – Degradability
• Influenced by – Host genotype – Dose and route of
administration – Presence of
adjuvants
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Epitopes • Discrete sites on immunogens recognized
by antibodies or T cell receptors • Macromolecules contain many epitopes with
different immunogenicities • Immunogenicity can vary between
individuals and populations. • Some epitopes are immunodominant
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• Identification of B-cell epitopes within the HPV16 E7 protein.
• Overlapping peptides were generated and injected into mice.
• The antibody response to each peptide was measured by ELISA.
• Comerford, S.A. et al. J. Virol. 65:4681-4690, 1991
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Epitopes • Overlapping • Non-overlapping
• Allosteric • Linear • Conformational • Neoantigen
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Antibodies
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Figure 4-7
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Secreted vs. Membrane Forms
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Antibody-Mediated Effector Functions
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Antibody Mediated Effector Functions
• Opsonization – IgG
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Antibody Mediated Effector Functions
• Opsonization – IgG
• Complement Activation IgM>IgG
• ADCC – IgG, IgE, IgA
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