Antibody Production Peptide Synthesis Peptide-Carrier Protein …1].pdf · 2021. 6. 10. ·...

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Antibody Production

Peptide Synthesis

Peptide-Carrier Protein Conjugation

Antibody Purification and Labeling

Catalog Antibodies

Premier Custom

Services

Ordering

Information

Peptide Synthesis

Antibody Production

Assay Development

Peptides

Antibodies

Fluorescent Probes

Assay Kits

Diverse Product

Portfolio

Highest Quality | Best Customer Support

Toll-Free: 800-452-5530Tel: 408-452-5055Fax: 408-452-5059E-mail: [email protected]: www.anaspec.comMail: AnaSpec, Inc.

2149 O'Toole Ave.San Jose, CA 95131USA

Table of Contents

I. Custom ServicesPolyclonal Antibody Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

Monoclonal Antibody Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

Phosphospecific Antibody Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Peptide Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Peptide-Carrier Protein Conjugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Antibody Purification and Labeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

II. Catalog ProductsApoptosis Related Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4

Cancer Research Related Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6

Signal Transduction Related Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8

Miscellaneous Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Secondary Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Whole Cell/Tissue Lysates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Antibody Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

Antibody Related Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

AnaSpec, Inc. 800-452-5530 www.anaspec.com 1

Table of Contents

In Need of Phosphospecific Antibodies?

AnaSpec offers a large selection of phospospecific antibodies including:

AKT IRS pSer SOX

ββ-Catenin JAK pThr STAT

ER P53 pTyr Tau

Phosphospecific antibodies are available in biotinylated and dye-labeled forms, aswell as AP/HRP and APC/B-PE/R-PE conjugates. AnaSpec also provides customphosphospecific antibody production; please contact [email protected] for moreinformation.

I. Custom Services

Polyclonal Antibody Production

AnaSpec provides polyclonal antibody production which includes:Free consultation for selection of epitope5 mg purified (>80% purity, up to 15 amino acids) peptide delivered to researcher for immediate study1 mg conjugated to BSA for ELISA screening; 4 mg conjugated to KLH for antibody productionImmunization of rabbits, mice, or chickens at your preferencePeptide-specific ELISA titration of antibody for all bleedsPre-immune samples of serum, ascites, or eggsShipment of 100 mL anti-serum, 20 mL ascites, or 12 antibody-containing eggs

Note: The rabbits can be maintained on a 3-4 wks cycle of immunization and bleed as required by the project.The results of your testing will determine whether the project should be continued, modified, or terminated

Monoclonal Antibody Production

AnaSpec provides the following hybridoma development program:Free consultation for epitope selectionEvaluation of suitable screening methodsFrequent communications between researcher and AnaSpec during hybridoma development processProject segmentation into different phases to provide researcher flexibility for terminating project at the end of each phaseFull confidentiality assured

Phase I: Immunogen Design and Immunization (9 wks)Design and preparation of protein antigen by researcher or peptide immunogen by AnaSpecSite-directed conjugation for peptide including peptide-KLH as antigen and peptide-BSA as screening reagentImmunization of 6 Balb/C mice, including maintenance and 3 immunizations for a standard 60-day protocolDetermination and qualification of titer of test bleed (e.g., ELISA, WB, IP)Shipment of all 6 mice test bleed at 1:100 dilution (1 mL each)

Phase II: Cell Fusion and Parental Clone Screening (3 wks)Mouse with the highest titer given final boost to establish murine myeloma cell lineFused cell clones screened by ELISA to select positive antibody-secreting parental clonesPotential parental clones expanded into 24-well plates for harvest of tissue culture supernatant for further antibody screening (ELISA, WB, or IHC)Shipment of 5-12 supernatants (2 mL/clone)

Phase III: Monoclonal Selection and Cryogenic Vial Preservation (3 wks)Monoclonal selection performed with 1-5 parental clonesSubclone with high specificity expanded and cryopreserved (4-5 vials each)Shipment of hybridoma cells and up to 10 mL of supernatant from each subclone

Phase IV: Large Scale Antibody Production (3 wks)Production performed either in vivo (ascites) or in vitro (bioreactor, stationary cell culture).

In vivo ascites production performed with Balb/C or Nu/Nu mouseTime frame for ascites production approx. 3 wksShipment of 25 mL of ascites from selected monoclone

AnaSpec, Inc. 800-452-5530 www.anaspec.com2

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In vitro antibody productionBioreactor: up to 10 mL of concentrated tissue culture supernatantStationary cell culture: up to 50 mL of tissue culture supernatant

Note: AnaSpec provides both small- and large-scale monoclonal antibody production.

Phosphospecific Antibody Production

AnaSpec provides phosphospecific antibody production which includes:15 mg purified (>90%, up to 15 amino acids) phosphopeptide synthesis --5 mg used for phosphospecific epitope affinity purification, 4 mg for KLH conjugationused for antibody production, 1 mg for BSA conjugation, and 5 mg delivered toresearcher11 mg purified (>80%, up to 15 amino acids) non-phosphopeptide synthesis --5 mg used for affinity depletion, 1 mg for BSA conjugation, and 5 mg delivered toresearcherImmunization of 2 rabbitsPhosphopeptide and non-phosphopeptide ELISA titration of antibody for all bleedsPre-immune sample of serum (5 mL/each rabbit) and anti-serum (100 mL total)Affinity purification of selected batch of serumDepletion of nonphospecific peptide matrixShipment of purified phosphospecific antibody which does not cross react withnon-phosphopeptides (confirmed by ELISA)

Peptide Synthesis

AnaSpec offers the following high-quality peptide synthesis:Synthesis of peptides with HPLC and MS certificationSynthesis of long peptides (>70 amino acids)Synthesis of high purity peptides (>90%, >95%)Peptide modification (acetylation, phosphorylation, etc.)Peptide labeling (biotin, dye, etc.)Peptide library

Note: If you would like to purchase an antibody package, please add a cysteine at either the -COOH or -NH2terminus for site-directed conjugation, unless you specify the conjugation through a free -COOH or -NH2 group.

Peptide-Carrier Protein Conjugation

AnaSpec offers the following peptide-carrier protein conjugation:Peptide-KLH conjugationPeptide-BSA conjugationPeptide-GGG conjugation

Note: Average coupling efficiency is between 80-95% (HPLC analysis).

Antibody Purification and Labeling

Epitope affinity purificationPreparation of affinity matrixProtein A/G purificationELISA titering of peptide-antibodySDS-PAGE analysis of antibodyAntibody labeling with:

Horseradish Peroxidase (HRP)Alkaline Phosphatase (AP)BiotinFluorochromesAPC, B-PE, R-PE

*Please call for the price and the specifics of each service or contact [email protected].


Custom Services

AAppooppttoossiiss RReellaatteedd AAnnttiibbooddiieess

Anti-Acinus (CP) hu WB 100 µg 28025 $220

Anti-Acinus (CT) hu WB 100 µg 28026 $220

Anti-Acinus (IN) hu WB 100 µg 28027 $220

Anti-Acinus (NP) hu WB 100 µg 28028 $220

Anti-ADAM10/KUZ (CT) hu WB 100 µg 28071 $220

Anti-AIF (CT) hu, ms, rt WB 100 µg 28029 $220

Anti-AIF (IN) hu, ms, rt WB 100 µg 28030 $220

Anti-AIF (NT) hu WB 100 µg 28031 $220

Anti-Apaf-1 (CT) hu WB 100 µg 28054 $240

Anti-Apaf-1 (NT) hu, ms, rt WB 100 µg 28055 $240

Anti-APRIL (ED) hu WB 100 µg 28032 $235

Anti-APRIL (ED2) hu WB 100 µg 28033 $235

Anti-ARC (CT) hu WB 100 µg 28084 $220

Anti-ARC (NT) hu, ms, rt WB 100 µg 28085 $220

Anti-ASC hu WB 100 µg 28096 $210

Anti-ASK (IN) hu, ms WB 100 µg 29680 $210

Anti-ASK1/MAPKKK5 (CT) hu WB 100 µg 28076 $220

Anti-Bad ms, rt WB, IHC 100 µg 29753 $205

Anti-BAFF/BLys/Thank/Tall-1 (CT) hu, ms, rt WB 100 µg 28072 $220

Anti-Bag-1 hu, ms, rt WB 100 µg 29754 $225

Anti-Bax hu, ms, rt WB 100 µg 29670 $220

Anti-Bcl10/CIPER/CLAP (NT) hu WB, IHC 100 µg 28057 $220

Anti-Bcl S/L hu, ms, rt WB, IHC 100 µg 29752 $215

Anti-Bim/BOD (IN) hu, ms, rt WB 100 µg 28058 $220

Anti-Bnip3L/Nix (IN) hu WB 100 µg 28059 $220

Anti-CAD/DFF40 (CT) ms WB 100 µg 28034 $230

Anti-CAD/DFF40 (I17) ms, rt WB 100 µg 28035 $230

Anti-Capase 1 hu WB, IP, IHC 100 µg 29755 $195

Anti-Capase 2 hu, ms, rt WB, IP, IHC 100 µg 29756 $195

Anti-Capase 3 hu WB 100 µg 29757 $195

Anti-Caspase 7 hu WB, IHC 100 µg 29556 $210


Anti-Caspase 8L hu WB 100 µg 29625 $210


Anti-Caspase 9/Mch6/Apaf-3 (IN1) hu WB 100 µg 28047 $240

Anti-Caspase 9/Mch6/Apaf-3 (IN2) hu WB 100 µg 28048 $240

Anti-Caspase 10/FLICE2 (CT) hu, ms, rt WB 100 µg 28049 $240

Anti-Caspase 12 (IN) hu, ms, rt WB, IHC 100 µg 28050 $230

Anti-Caspase 12 (NT) hu, ms, rt WB 100 µg 28051 $230

Anti-Caspase 13/ERICE hu, ms, rt WB 100 µg 28052 $230

Monoclonal Anti-CD1a hu IHC 100 µg 29684 $195

Anti-CD2 hu IHC 100 µg 29685 $195

Anti-CD3 hu, ms IHC 100 µg 29588 $195

Monoclonal Anti-CD4 hu IHC 100 µg 29683 $195

Anti-CD5 hu IHC 100 µg 29589 $195

Anti-CD8 ms IHC 100 µg 29590 $195


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Product Species Application Size Catalog # US$

II. Catalog Products

Monoclonal Anti-CD10 hu, rt IHC 100 µg 29681 $195

Anti-CD20 hu IHC 100 µg 29587 $195

Anti-CD23 hu WB, IHC 100 µg 29682 $195





Anti-CD40 hu, ms, rt WB, IP, IHC 100 µg 29690 $195





Anti-CD79a hu, ms IHC 100 µg 29560 $215

Anti-CD95/Fas hu IHC 100 µg 29561 $215


Anti-CD117/c-kit hu IHC 100 µg 29562 $215

Anti-CIDE-A (CT) hu WB 100 µg 28036 $220

Anti-CIDE-A (CT) ms WB 100 µg 28037 $220

Anti-CIDE-B (CT) ms WB 100 µg 28038 $220

Anti-Cyclin A hu, ms, rt WB, IHC 100 µg 29758 $225

Anti-Cyclin B1 hu WB, IHC 100 µg 29759 $225

Anti-Cyclin D1 hu, ms, rt WB, IHC 100 µg 29760 $225

Anti-Cyclin E hu, ms, rt WB, IHC 100 µg 29761 $215

Anti-D4-GDI hu WB, IHC 100 µg 29563 $195

Anti-DAPK2 hu, ms, rt WB 100 µg 28077 $220

Anti-Daxx (CT) hu, ms, rt WB 100 µg 28097 $220

Anti-DcR1/TRAIL-R3 (ED) hu, ms, rt WB 100 µg 28060 $230

Anti-DcR1/TRAIL-R3 (ED2) hu, ms, rt WB 100 µg 28061 $230

Anti-DcR2/TRAIL-R4 (ID) hu, ms, rt WB 100 µg 28062 $230

Anti-DcR3/TR6 (NT) hu, ms, rt WB 100 µg 28073 $230

Anti-DEFCAP (CT) hu WB 100 µg 28053 $210

Anti-DFF40/CAD (I18) hu, ms, rt WB 100 µg 28039 $230

Anti-DFF40/CAD (IN) hu WB 100 µg 28040 $230

Anti-DFF40/CAD (NT) hu WB 100 µg 28041 $230

Anti-DFF45/ICAD (CT) hu WB 100 µg 28042 $230

Anti-DFF45/ICAD (NT) hu WB, IHC 100 µg 28043 $230

Anti-DNase II (CT) hu WB 100 µg 28044 $220

Anti-DR3/Wsl-1/Apo-3 (CT) hu, ms WB 100 µg 28067 $230

Anti-DR3/Wsl-1/Apo-3 (ED) hu, ms WB 100 µg 28068 $230

Anti-DR3/Wsl-1/Apo-3 (NT) hu, ms WB 100 µg 28069 $230

Anti-DR4/TRAIL-R1 (CT) hu WB 100 µg 28063 $230

Anti-DR4/TRAIL-R1 (NT) hu WB 100 µg 28064 $230

Anti-DR5/TRAIL-R2 (CT) hu, ms WB 100 µg 28065 $230

Anti-DR6 (NT) hu WB 100 µg 28070 $230

Anti-DRAK1 (NT) hu WB 100 µg 28078 $220

Anti-DRAK2 (CT) hu WB 100 µg 28079 $220

Anti-F1A (CT) hu, ms, rt WB 100 µg 28098 $220

Anti-FLASH (CT) hu WB 100 µg 28056 $220


Catalog Products


Anti-FLIP/I-FLICE (CT) ms WB 100 µg 28086 $230

Anti-FLIP/I-FLICE (CT) hu, ms, rt WB 100 µg 28087 $230

Anti-FLIP/I-FLICE (NT) hu, ms, rt WB 100 µg 28088 $230

Anti-FLIPs (CT) hu WB 100 µg 28089 $230

Anti-ICAD/DFF45 (CT) ms WB, IHC 100 µg 28045 $230

Anti-ICAD/DFF45 (NT) ms WB, IHC 100 µg 28046 $230

Anti-ICEBERG (CT) hu WB 100 µg 28090 $210

Anti-mSmac/DIABLO (CT) hu, ms, rt WB, IHC 100 µg 28092 $240

Anti-mSurvivin/TIAP (CT) ms WB, IHC 100 µg 28095 $240

Anti-P16 hu WB, IHC 100 µg 29745 $195

Anti-P21 hu, ms, rt WB, IHC 100 µg 29746 $195

Anti-P27 hu WB 100 µg 29762 $195




Anti-RAIDD/CRADD (CT) hu WB 100 µg 28099 $220

Anti-RAIDD/CRADD (IN) hu WB 100 µg 28100 $220

Anti-Rho hu, ms, rt WB 100 µg 29679 $220

Anti-RICK/RIP2 (CT) hu WB 100 µg 28080 $220

Anti-RICK/RIP2 (NT) hu, ms, rt WB 100 µg 28081 $220

Anti-RIP3 hu, rt WB 100 µg 28082 $210

Anti-Smac/DIABLO (CT) hu, ms, rt WB 100 µg 28091 $220

Anti-SODD (NT) hu, ms, rt WB 100 µg 28074 $220

Anti-Survivin/TIAP (CT) hu WB, IHC 100 µg 28093 $240

Anti-Survivin/TIAP (NT) hu WB, IHC 100 µg 28094 $240

Anti-TACE/adam 17 (CT) hu, ms, rt WB 100 µg 28075 $220

Anti-TRAIL/Apo-2L (CT) hu WB 100 µg 28066 $220

Anti-ZIPK (IN) hu, ms, rt WB 100 µg 28083 $220

CCaanncceerr RReesseeaarrcchh RReellaatteedd AAnnttiibbooddiieess

Anti-Actin, smooth muscle hu IHC 100 µg 29553 $210

Anti-ALK/p80 hu IHC 100 µg 29584 $195

Anti-β-Amyloid hu WB, IHC 100 µg 28182 $240

Anti-BCL-2 (pTyr129), phospho-specific bv, hu, ms, rt WB, IP 100 µg 29658 $210

Anti-BCL-2 (Paired129), non-phospho-specific bv, hu, ms, rt WB, IP 100 µg 29659 $210

Anti-Cadherin pan hu IHC 100 µg 29569 $195

Anti-Calcitonin hu IHC 100 µg 29555 $230

Anti-Calretinin hu IHC 100 µg 29586 $240

Anti-β-Catenin hu WB, IP, IHC 100 µg 29554 $210

Anti-β-Catenin (pSer675), phospho-specific ck, fr, hu, ms, rt WB, IP 100 µg 29619 $210

Anti-β-Catenin (Paired675), non-phospho-specific ck, fr, hu, ms, rt WB, IP 100 µg 29620 $210

Anti-C-erbB-2 hu IHC 100 µg 29559 $195

Anti-Chk2 (NT) hu, ms, rt WB 100 µg 28000 $210

Anti-Chromogranin A hu IHC 100 µg 29591 $195

Monoclonal Anti-c-Myc (pSer62) ca, hu, ms, rt sh WB 100 µg 29527 $210

Anti-Cytochrome C hu IHC 100 µg 28184 $210

Anti-Desmin hu, ms, rt IHC 100 µg 29593 $210

Anti-EGFR hu, ms WB, IHC 100 µg 29564 $195


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Anti-EGFR (phospho) hu, ms, rt WB 100 µg 29615 $195

Anti-ER hu WB, IHC 100 µg 29595 $210

Anti-ER-alpha (pSer167), phospho-specific hu WB, IP 100 µg 28001 $210

Anti-ER-alpha (Paired167), non-phospho-specific hu WB 100 µg 28002 $210

Anti-ER-beta (IN) hu, ms, rt WB, IP, IHC 100 µg 28003 $210

Anti-Heregulin hu WB, IHC 100 µg 29566 $210

Anti-MAGE (IN) hu WB, IHC 100 µg 28004 $195

Anti-MMP-1 hu WB 100 µg 29574 $210

Anti-MMP-2 hu, ms, rt WB 100 µg 29575 $210



Anti-MMP-8 hu WB 100 µg 29578 $205

Anti-MMP-9 hu WB 100 µg 29579 $240

Anti-MMP-10 hu WB 100 µg 29580 $220

Anti-MMP-16 hu WB 100 µg 29581 $220

Anti-MTBP (IN2) hu WB 100 µg 28005 $195

Anti-P53 (CT) hu IHC 100 µg 29594 $235

Anti-P53 (pSer6), phospho-specific hu WB, IP 100 µg 28006 $235

Anti-P53 (Paired6), non-phospho-specific hu WB, IP 100 µg 28007 $235

Anti-P53 (pSer9), phospho-specific hu WB 100 µg 28008 $235

Anti-P53 (Paired9), non-phospho-specific hu WB 100 µg 28009 $235

Anti-P53 (pSer15), phospho-specific hu WB, IP 100 µg 28010 $235

Anti-P53 (Paired15), non-phospho-specific hu WB 100 µg 28011 $235

Anti-P53AIP1 (CT) hu WB 100 µg 28013 $235

Anti-P53R2 (NT) hu, ms, rt WB 100 µg 28012 $235

Anti-PID/MTA2 hu, ms, rt WB 100 µg 28014 $210

Anti-PLAP hu IHC 100 µg 29598 $195

Anti-PR hu IHC 100 µg 29597 $195

Monoclonal Anti-pTEN hu WB, IHC 100 µg 29528 $210

Anti-pTEN (pSer385), phospho-specific hu, ms, rt WB, IP 100 µg 29642 $210

Anti-pTEN (Paired385), non-phospho-specific hu, ms, rt WB, IP 100 µg 29643 $210

Anti-pTEN (CT) hu WB, IP 100 µg 28015 $210

Anti-Retinoblastoma hu WB, IP, IHC 100 µg 29570 $195

Anti-S100 hu IHC 100 µg 29571 $195

Anti-STEAP (NT) hu WB, IP, IHC 100 µg 28016 $195

Anti-Synuclein hu IHC 100 µg 28186 $195

Anti-Tau (Cocktail) hu WB 100 µg 29582 $210

Anti-Tau (pSer202), phospho-specific hu WB, IP 100 µg 28017 $210

Anti-Tau (Paired 202), non-phospho-specific hu WB 100 µg 28018 $210

Anti-Tau (pThr205), phospho-specific hu WB, IP 100 µg 28019 $210


Anti-Tau (pSer396), phospho-specific hu WB, IP 100 µg 28021 $210


Anti-Tau (pSer404), phospho-specific hu WB 100 µg 28023 $210


Anti-VEGF hu WB 100 µg 29573 $195


Catalog Products


SSiiggnnaall TTrraannssdduuccttiioonn RReellaatteedd AAnnttiibbooddiieessAnti-ACTH hu IHC 100 µg 29669 $210Anti-AKT1 (pThr308), phospho-specific hu WB 100 µg 28103 $195 Anti-AKT1 (Paired308), non-phospho-specific hu WB 100 µg 28104 $195 Anti-AKT1 (pSer473), phospho-specific hu WB 100 µg 28101 $195 Anti-AKT1 (Paired473), non-phospho-specific hu WB 100 µg 28102 $195 Anti-AKT2 (CT) hu, ms, rt WB, IP 100 µg 28105 $195 Anti-AKT3 (IN) hu, ms, rt WB, IP 100 µg 28106 $195 Anti-Androgen Receptor (phospho) hu WB 100 µg 29665 $240 Anti-APP (AβNT) hu, ms, rt WB 100 µg 28151 $220 Anti-APP (CT) hu, ms, rt WB 100 µg 28152 $220 Anti-BACE/Asp2 (CT) hu, ms WB 100 µg 28154 $220 Anti-BACE2/Asp1 (CT) hu, ms, rt WB 100 µg 28156 $220 Anti-BACE2/Asp1 (NT) hu, ms, rt WB 100 µg 28155 $220 Anti-Bonzo/STRL33 (CT) hu WB 100 µg 28141 $220 Anti-Bonzo/STRL33 (NT2) hu WB 100 µg 28142 $220 Monoclonal Anti-cAMP hu, ms, rt WB 100 µg 29539 $195 Monoclonal Anti-cAMP, 5-FAM-Labeled hu, ms, rt WB 50 µg 29540 $195 Anti-CCR5 (NT) hu WB 100 µg 28143 $230 Anti-CCR8 (EL) hu WB 100 µg 28144 $230 Anti-CIS-1 (IN) hu WB 100 µg 28137 $195 Anti-CSF-1R (pTyr723), phospho-specific hu, ms WB, IP 100 µg 29646 $195 Anti-CSF-1R (Paired723), non-phospho-specific hu, ms WB, IP 100 µg 29647 $195 Anti-CX3CR1/V28 (EL) hu WB 100 µg 28145 $230 Anti-CX3CR1/V28 (NT) hu, ms, rt WB, FACS 100 µg 28146 $230 Anti-CXCR4 (EL) hu, ms WB 100 µg 28147 $230 Anti-CXCR4 (NT) hu, ms WB, IP, IHC 100 µg 28148 $230 Anti-Cyclin D1 hu IHC 100 µg 29592 $195 Anti-DOK (CT) hu WB, IP 100 µg 28107 $220 Anti-Eotaxin (CT) hu WB 100 µg 28149 $220 Anti-Factor XIIIa hu WB, IHC 100 µg 29565 $195 Monoclonal Anti-FSH hu WB, IHC 100 µg 29530 $195 Anti-GABAa Receptor av, bv, hu, ms, rt WB, IHC 100 µg 29585 $230 Anti-GFR-1/GDNFR (IN) hu, ms, rt WB 100 µg 28157 $220 Anti-GFR-2/GDNFR (IN) hu, ms, rt WB 100 µg 28158 $220 Anti-GFR-3/GDNFR (IN) hu, ms, rt WB 100 µg 28159 $220 Anti-Glucagon hu WB 100 µg 29667 $195 Anti-GPR15/BOB (NT) hu, ms, rt WB 100 µg 28150 $220 Anti-GR (pSer211), phospho-specific hu, ms WB, IP 100 µg 29654 $210 Anti-GR (Paired211), non-phospho-specific hu, ms WB, IP 100 µg 29655 $210 Anti-GR (pSer515), phospho-specific ms, rt WB, IP 100 µg 29656 $210 Anti-GR (Paired515), non-phospho-specific ms, rt WB, IP 100 µg 29657 $210 Anti-GRSF-1 (pThr290), phospho-specific hu, ms, rt WB, IP 100 µg 29660 $195 Anti-GRSF-1 (Paired290), non-phospho-specific hu, ms, rt WB, IP 100 µg 29661 $195 Anti-IκB-α (pSer32/36), phospho-specific hu, ms, rt WB, IP 100 µg 29626 $210 Anti-IκB-α (Paired32/36), non-phospho-specific hu, ms, rt WB, IP 100 µg 29627 $210 Anti-αIKK/IKK-1 (C1) hu WB, IHC 100 µg 28108 $195 Anti-αIKK/IKK-1 (C2) hu WB 100 µg 28109 $195 Anti-αIKK/IKK-1 (C3) hu WB 100 µg 28110 $195


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Anti-βIKK/IKK-2 (C3) hu WB 100 µg 28111 $195 Anti-εIKK/IKKi (CT) hu WB 100 µg 28112 $195 Anti-γIKK/NEMO/FIP3 (NT) hu, ms, rt WB 100 µg 28113 $195 Anti-IL-1RAcP (C1) hu WB 100 µg 28114 $220 Anti-IL-1RAcP (C2) hu WB 100 µg 28115 $220 Anti-IRAK (CT) hu, ms, rt WB, IP 100 µg 28116 $230 Anti-IRAK-2 (C2) hu WB 100 µg 28117 $230 Anti-IRAK-2 (CT) hu WB 100 µg 28118 $230 Anti-IRAK-M hu, ms, rt WB 100 µg 28119 $230 Anti-IRS-1 (pSer312), phospho-specific hu, ms, rt WB, IP 100 µg 28120 $195 Anti-IRS-1 (Paired312), non-phospho-specific hu WB 100 µg 28121 $195 Anti-IRS-2 (pSer731), phospho-specific hu, ms, rt WB, IP 100 µg 28122 $195 Anti-IRS-2 (Paired731), non-phospho-specific hu WB 100 µg 28123 $195 Anti-JAK2 (pTyr1007/1008), phospho-specific hu, ms, rt WB 100 µg 29640 $210 Anti-JAK2 (Paired1007/1008), non-phospho-specific hu, ms, rt WB 100 µg 29641 $210 Anti-Ki-67 hu IHC 100 µg 29567 $195 Anti-KPI-2 hu WB 100 µg 29624 $195 Anti-JAK1 hu, ms, rt WB, IP 100 µg 29763 $215 Anti-JNK hu, ms, rt WB, IP 100 µg 29764 $215 Anti-LSP (pSer252), phospho-specific hu WB, IP 100 µg 28161 $195 Anti-LSP (Paired252), non-phopho-specific hu WB 100 µg 28162 $195 Anti-MADD/DENN (CT) hu, ms, rt WB 100 µg 28124 $195 Anti-MEK-5/MAPKK-5 (phospho) hu, ms, rt WB 100 µg 29770 $225 Anti-MEK-5/MAPKK-5 hu, ms, rt WB 100 µg 29771 $215 Anti-MYD88 (CT) hu WB 100 µg 28125 $220 Anti-MYD88 (IN) hu, ms WB 100 µg 28126 $220 Anti-NAK/TBK1 (CT) hu, ms WB 100 µg 28127 $220 Anti-Neurabin (NT) hu, ms, rt WB 100 µg 29635 $195 Anti-Neurabin (pThr900), phospho-specific hu, ms, rt WB, IP 100 µg 29633 $195 Anti-Neurabin (Paired900), non-phospho-specific hu, ms, rt WB, IP 100 µg 29634 $195 Anti-Neurotensin Receptor hu WB 100 µg 29664 $195 Anti-Neuturin (CT) hu WB 100 µg 28160 $220 Anti-NFκBp50 hu WB, IHC 100 µg 29698 $195 Anti-NFκBp65 hu WB, IHC 100 µg 29697 $195 Anti-NFkBp65 (pSerS276), phospho-specific hu WB 100 µg 29775 $225 Anti-NFkBp65 (PairedS276), non-phospho-specific hu WB 100 µg 29776 $215 Anti-NIK (CT) hu WB 100 µg 28128 $220 Anti-NOS (pSer116), phospho-specific bv WB, IP 100 µg 28129 $210 Anti-NOS (Paired116), non-phospho-specific bv WB 100 µg 28130 $210 Anti-p14ARF hu WB, IP 100 µg 29568 $205 Anti-P70S6K (pSer434), phospho-specific hu, ms, rt WB, IP 100 µg 29621 $195 Anti-P70S6K (Paired434), non-phospho-specific hu, ms, rt WB, IP 100 µg 29622 $195 Anti-PAK (pSer474), phospho-specific hu, ms, rt WB, IP 100 µg 29662 $195 Anti-PAK (Paired474), non-phospho-specific hu, ms, rt WB, IP 100 µg 29663 $195 Anti-PKC (pSer345), phospho-specific hu, ms, rt WB, IP 100 µg 29644 $195 Anti-PKC (Paired345), non-phospho-specific hu, ms, rt WB, IP 100 µg 29645 $195 Monoclonal Anti-pSer hu, ms, rt WB, IP, IHC 100 µg 29524 $240 Monoclonal Anti-pSer, Biotinylated hu, ms, rt WB 50 µg 29534 $195 Monoclonal Anti-pSer, AMCA-X-Labeled hu, ms, rt WB, IHC 50 µg 29536 $240 Monoclonal Anti-pSer, 5-FAM-Labeled hu, ms, rt WB, IHC 50 µg 29532 $240


Catalog Products


Monoclonal Anti-pSer, 5-TAMRA-Labeled hu, ms, rt WB, IHC 50 µg 29535 $240 Anti-pSer all WB, IP, IHC 50 µg 29675 $240 Monoclonal Anti-pThr hu, ms, rt WB 100 µg 29529 $240 Anti-pThr all WB 50 µg 29676 $240 Anti-PTP 1c (pSer591), phospho-specific hu, ms WB, IP 100 µg 29652 $195 Anti-PTP 1c (Paired591), non-phospho-specific hu, ms WB, IP 100 µg 29653 $195 Anti-pTyr all WB 50 µg 29677 $220 Anti-RAGE bv, hu, ms, rt WB 100 µg 29618 $210 Anti-Rhα1GF-1R (phospho) hu, ms, rt WB 100 µg 29772 $225 Anti-Rhα1GF-1R hu, ms, rt WB 100 µg 29773 $215 Anti-Rho-Kinase (pThr396), phospho-specific hu, ms, rt WB 100 µg 29648 $195 Anti-Rho-Kinase (Paired396), non-phospho-specific hu, ms, rt WB 100 µg 29649 $195 Anti-SIRPα/SHPS-1 (CT) hu, ms, rt WB 100 µg 28131 $220 Anti-SOCS-1 (CT) hu WB, IP 100 µg 28132 $195 Anti-SOCS-2 (CT) hu WB, IP 100 µg 28133 $195 Anti-SOCS-3 (CT) hu WB, IP 100 µg 28134 $195 Anti-SOCS-4 (IN) hu WB 100 µg 28135 $195 Anti-SOCS-5 (CT) ms WB 100 µg 28136 $195 Anti-SOX 9 (pSer181), phospho-specific hu WB 100 µg 28138 $195 Anti-SOX 9 (Paired181), non-phospho-specific hu WB 100 µg 28139 $195 Anti-STAT1 (CT) hu, ms, rt WB, IP 100 µg 28140 $210 Anti-STAT1 (pTyr701), phospho-specific ck, hu, ms, rt WB, IP 100 µg 29636 $210 Anti-STAT1 (Paired701), non-phospho-specific ck, hu, ms, rt WB, IP 100 µg 29637 $210 Anti-STAT2 hu WB, IP, IHC 100 µg 29765 $215 Anti-STAT3 hu, ms, rt WB, IP, IHC 100 µg 29699 $210 Anti-STAT4 hu, ms, rt WB, IP, IHC 100 µg 29766 $215 Anti-STAT5 (pTyr694), phospho-specific hu, ms, rt WB, IP 100 µg 29638 $210 Anti-STAT5 (Paired694), non-phospho-specific hu, ms, rt WB, IP 100 µg 29639 $210 Anti-STAT5a hu, ms, rt WB, IP, IHC 100 µg 29767 $215 Anti-STAT5b hu, ms, rt WB, IP, IHC 100 µg 29768 $215 Anti-STAT6 hu WB 100 mg 29769 $215 Anti-Synaptophysin hu IHC 100 µg 29596 $195 Anti-TEF-1 hu, ms WB, IHC 100 µg 29623 $195 Anti-Tgase II hu WB, IP, IHC 100 µg 29572 $195 Anti-Ubiquitin bv, ck, hu, ms, rt WB, IHC 100 µg 29599 $195 Anti-Vasopressin V1b Receptor hu WB 100 µg 29671 $195

MMiisscceellllaanneeoouuss AAnnttiibbooddiieessAnti-C-Myc Tag WB 100 µg 29774 $195 Anti-FLAG WB 100 µg 29674 $95 Monoclonal Anti-GST WB, IHC 100 µg 29531 $150 Anti-GFP WB, IP, IHC 100 µg 29779 $195 Anti-HA Tag WB 100 µg 29629 $150 Anti-His Tag WB, IHC 100 µg 29673 $195 Anti-HSV WB, IP 100 µg 29743 $215 Anti-NSAP1/hnRNP hu WB 100 µg 29678 $220 Anti-SARS (Cocktail) WB 100 µg 29628 $195 Anti-SARS (IN1) WB 100 µg 29630 $195 Anti-SARS (IN2) WB 100 µg 29631 $195 Anti-SARS (IN3) WB 100 µg 29632 $195


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SSeeccoonnddaarryy AAnnttiibbooddiieess

Rabbit Anti-Mouse IgG (H+L), purified 1 mg 28164 $75

Rabbit Anti-Mouse IgG (H+L), HRP-Conjugated 0.5 mg 28165 $70

Rabbit Anti-Mouse IgG (H+L), AP-Conjugated 0.5 mg 28166 $85

Rabbit Anti-Mouse IgG (H+L), Biotinylated 0.5 mg 28167 $70

Rabbit Anti-Goat IgG (H+L), purified 1 mg 28168 $75

Rabbit Anti-Goat IgG (H+L), HRP-Conjugated 0.5 mg 28169 $70

Rabbit Anti-Goat IgG (H+L), AP-Conjugated 0.5 mg 28170 $85

Rabbit Anti-Goat IgG (H+L), Biotinylated 0.5 mg 28171 $70

Goat Anti-Mouse IgG (H+L), purified 1 mg 28172 $65

Goat Anti-Mouse IgG (H+L), HRP-Conjugated 0.5 mg 28173 $60

Goat Anti-Mouse IgG (H+L), AP-Conjugated 0.5 mg 28174 $85

Goat Anti-Mouse IgG (H+L), Biontinylated 0.5 mg 28175 $70

Goat Anti-Rabbit IgG (H+L), purified 1 mg 28176 $65

Goat Anti-Rabbit IgG (H+L), HRP-Conjugated 0.5 mg 28177 $60

Goat Anti-Rabbit IgG (H+L), AP-Conjugated 0.5 mg 28178 $85

Goat Anti-Rabbit IgG (H+L), Biotinylated 0.5 mg 28179 $70

Rabbit Anti-Chicken IgY, purified 1 mg 29709 $75

HRP-Rabbit Anti-Chicken IgY, HRP-Conjugated 0.5 mg 29710 $75

Rabbit Anti-Chicken IgY, AP-Conjugated 0.5 mg 29711 $85

Rabbit anti-Chicken IgY, Biotinylated 0.5 mg 29712 $80

WWhhoollee CCeellll//TTiissssuuee LLyyssaatteess

K562 Whole Cell Lysate 200 µg 29501 $75

Jurkat Whole Cell Lysate 200 µg 29502 $75

HL60 Whole Cell Lysate 200 µg 29503 $75

SW1353 Whole Cell Lysate 200 µg 29504 $75

Mouse Spleen Tissue Lysate 200 µg 29505 $75

Raji Whole Cell Lysate 200 µg 29506 $75

Mouse Brain Tissue Lysate 200 µg 29507 $75

Mouse Muscle Tissue Lysate 200 µg 29508 $75

Mouse Liver Tissue Lysate 200 µg 29509 $75

Mouse Lung Tissue Lysate 200 µg 29510 $75

Mouse Heart Tissue Lysate 200 µg 29511 $75

Mouse Retina Tissue Lysate 200 µg 29512 $75

Mouse Intestine Tissue Lysate 200 µg 29513 $75

A431 Whole Cell Lysate 200 µg 29514 $75

3T3 Whole Cell Lysate 200 µg 29515 $75

Lovo Whole Cell Lysate 200 µg 29516 $75

Hela Whole Cell Lysate 200 µg 29517 $75

THP-1 Whole Cell Lysate 200 µg 29518 $75

MCF-7 Whole Cell Lysate 200 µg 29519 $75

HEK293 Whole Cell Lysate 200 µg 29520 $75

10TY2 Whole Cell Lysate 200 µg 29521 $75

STO Whole Cell Lysate 200 µg 29522 $75


Catalog Products

Product Size Catalog # US$

AAnnttiibbooddyy RReeaaggeennttss

Antibody Diluent (Ready to Use) 125 mL 29704 $50

AnaPrep™ IHC Histain Kit-AEC 15 mL 29702 $215

AnaPrep™ AEC Substrate System 125 mL 29703 $155

AnaPrep™ IHC Histain Kit-DAB 15 mL 29700 $215

AnaPrep™ DAB Substrate System 125 mL 29701 $105

Maleimidyl KLH 5 mg 29551 $120

Maleimidyl BSA 5 mg 29552 $120

AAnnttiibbooddyy RReellaatteedd KKiittss

AnaPrep™ Maleimide Activated KLH Conjugation Kit 1 kit 29722 $265

AnaPrep™ Maleimide Activated BSA Conjugation Kit 1 kit 29723 $250

AnaPrep™ Maleimide Activated Antigen Conjugation Kit 1 kit 29724 $350

AnaPrep™ HA Fusion Protein Purification Kit 1 kit 29717 $285

AnaPrep™ His Fusion Protein Purification Kit 1 kit 29716 $285

AnaTag™ Neuron Staining Kit 1 kit 29715 $285

AnaTag™ AMCA-X Protein Labeling Kit 1 kit 71000 $195 AnaTag™ 5-FAM Protein Labeling Kit 1 kit 71001 $195 AnaTag™ 5-TAMRA Protein Labeling Kit 1 kit 71002 $195 AnaTag™ 5-ROX Protein Labeling Kit 1 kit 71005 $195 AnaTag™ HiLyte Fluor™ TR Protein Labeling Kit 1 kit 71006 $195 AnaTag™ HiLyte Fluor™ 555 Protein Labeling Kit 1 kit 71007 $295 AnaTag™ HiLyte Fluor™ 647 Protein Labeling Kit 1 kit 71008 $295 AnaTag™ Biotin Protein Labeling Kit 1 kit 71003 $195 AnaTag™ AP Protein Labeling Kit 1 kit 71009 $295 AnaTag™ HRP Protein Labeling Kit 1 kit 71010 $295 AnaTag™ APC Protein Labeling Kit 1 kit 71011 $295 AnaTag™ B-PE Protein Labeling Kit 1 kit 71012 $395 AnaTag™ R-PE Protein Labeling Kit 1 kit 71013 $395


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Species: av=avian, bv=bovine, ca=canine, ck=chicken, fr=frog, hu=human, ms=mouse, rt=rat, sh=sheep.Application: WB=Western Blot; IP=Immunoprecipitation; IHC=Immunohistochemistry.*Polyclonal antibodies are generated in rabbits and monoclonal antibodies are generated in mice.

Product Size Catalog # US$

Primary and secondary antibodies are available in biotinylated and dye-labeled forms, as well as AP/HRPand APC/B-PE/R-PE conjugates; please contact [email protected] for more information.

Custom Antibody Production Order FormPlease photocopy this form and fill out the following information, then return the formto us by mail or via fax (408-452-5059). You can also place your order on-line atwww.anaspec.com. If you prefer to receive a price quotation before ordering, pleasecheck here .

Date:_________________________ P.O. #:_________________________Name:_______________________________________________________________Company/Institution:____________________________________________________Phone #:______________________ Fax #:_________________________

Bill to Address: Ship to Address:______________________________ ____________________________________________________________ ____________________________________________________________ ____________________________________________________________ ______________________________

Alanine Ala (A)Arginine Arg (R)Asparagine Asn (N)Aspartic Acid Asp (D)Cysteine Cys (C)Glutamic Acid Glu (E)Glutamine Gln (Q)

Glycine Gly (G)Histidine His (H)Isoleucine Ile (I)Leucine Leu (L)Lysine Lys (K)Methionine Met (M)Phenylalanine Phe (F)

Proline Pro (P)Serine Ser (S)Threonine Thr (T)Tryptophan Trp (W)Tyrosine Tyr (Y)Valine Val (V)

Antibody Reference #:__________________________________________________Sequence:___________________________________________________________________________________________________________________________________________________________________________________________________Requirements:________________________________________________________________________________________________________________________________________________________________________________________________

Polyclonal Antibody ProductionMonoclonal Antibody ProductionPhosphospecific Antibody ProductionAntibody Purification / please specify:____________________________________________Antibody Labeling / please specify:______________________________________________Other / please specify:__________________________________________________________

Choice of Animal for Immunization:

Rabbit Mouse Chicken

AnaSpec, Inc.

2149 O’Toole Ave.

San Jose, CA 95131

800-452-5530

408-452-5055

Fax 408-452-5059

[email protected]

www.anaspec.com

©2004 AnaSpec, Inc.International Version

Antibody Production Peptide Synthesis Peptide-Carrier Protein …1].pdf · 2021. 6. 10. ·...

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Transcript of Antibody Production Peptide Synthesis Peptide-Carrier Protein …1].pdf · 2021. 6. 10. ·...