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MONOCLONAL ANTIBODY For research use only. Not for clinical diagnosis. Catalog No. TSS-M01 Anti CRF21 BACKGROUND This protein is encoded by the gene (C7orf24) existent in open reading frame24 of the 7 th chromosome and is called CRF21 (cytochrome c-releasing factor), γ-glutamylcyclotransferase (an essential enzyme for the γ-glutamyl cycle), or U7 (highly expressed in a variety of carcinoma cells). Product type Monoclonal antibodies Host Mouse Source Purified Ascites Form Liquid Volume Concentration 100 ug 1 mg/ml Specificity human C7orf24 protein Antigen Recombinant human C7orf24 protein Isotype IgG1 Clone:6-1E Application notes WB, IHC e s u d e d n e m m o c e R s n o i t u l i d d e d n e m m o c e R Western blotting: 0.2 ug/ml Immunohistochemistry: 40 ug/ml Optimal dilutions/concentrations should be determined by the end user. Cross reactivity React with human. Do not cross react with mouse. Not test for other species. Storage Store at 4°C References 1) Masuda Y, et.al. (2006) A novel 21-kDa cytochrome c-releasing factor is generated upon treatment of human leukemia U937 cells with geranylgeraniol. Biochem Biophys Res Commun. 346: 454-60. PMID: 16765912 2) Kageyama S, et al. (2007) A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma. Proteomics Clin Appl. 1: 192-9. PMID: 21136669 3) Oakley AJ, et al. (2008) The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma- glutamyl cycle. J Biol Chem. 283: 22031-42. PMID: 18515354 Anti CRF21, Cat# TSS-M01 1/2 Version# 2-190903

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  • MONOCLONAL ANTIBODY For research use only. Not for clinical diagnosis.Catalog No. TSS-M01

    Anti CRF21

    BACKGROUND This protein is encoded by the gene (C7orf24) existent in open reading frame24 of the 7th chromosome and is called CRF21 (cytochrome c-releasing factor), γ-glutamylcyclotransferase (an essential enzyme for the γ-glutamyl cycle), or U7 (highly expressed in a variety of carcinoma cells).

    Product type Monoclonal antibodies Host Mouse Source Purified Ascites Form Liquid Volume Concentration

    100 ug 1 mg/ml

    Specificity human C7orf24 protein Antigen Recombinant human C7orf24 protein Isotype IgG1 Clone:6-1E

    Application notes WB, IHC

    esu dednemmoceR

    snoitulid dednemmoceR Western blotting: 0.2 ug/ml Immunohistochemistry: 40 ug/ml Optimal dilutions/concentrations should be determined by the end user.

    Cross reactivity React with human. Do not cross react with mouse. Not test for other species.

    Storage Store at 4°C

    References

    1) Masuda Y, et.al. (2006) A novel 21-kDa cytochrome c-releasing factor is generated upon treatment of human leukemia U937 cells with geranylgeraniol. Biochem Biophys Res Commun. 346: 454-60. PMID: 16765912

    2) Kageyama S, et al. (2007) A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma. Proteomics Clin Appl. 1: 192-9. PMID: 21136669

    3) Oakley AJ, et al. (2008) The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma- glutamyl cycle. J Biol Chem. 283: 22031-42. PMID: 18515354

    Anti CRF21, Cat# TSS-M01 1/2 Version# 2-190903

    https://www.ncbi.nlm.nih.gov/pubmed/?term=16765912https://www.ncbi.nlm.nih.gov/pubmed/?term=21136669https://www.ncbi.nlm.nih.gov/pubmed/?term=18515354

  • For research use only. Not for clinical diagnosis.

    Clone 6.1E

    20

    30

    40

    50

    60

    kDa

    HE stain

    Example Assay Data

    Anti CRF21, Cat# TSS-M01 2/2 Version# 2-190903

    Fig.2 Immunohistochemical analysis of human breast cancer using anti CRF21 (Clone 6.1E) and HE stain.

    Fig.1 Western Blot analysisSample : Bladder cancer tissue lysate with anti CRF21 antibody.

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