ANTI-BACTERIAL POPERTIES OF CAJANUS CAJAN LEAF

ABOUT THE PLANT

Cajanus cajan, also called Pigeon Pea plant

Short-term perennial shrub Belongs to the Fabaceae family of the

Dicot group. Its rich in proteins, carbohydrates and

minerals Medicinal applications

IMPORTANT INFORMATION

Excellent source of anti-bacterial materials

Possible antibacterial component inside the leaf: Cajanuslactone

STREPTOCOCCUS MUTANS

Inhibits the human oral cavity Makes 36% of the total bacteria

inhibiting human teeth Heterotrophic organism Reproduce asexually Facultative anaerobe

OBJECTIVE

Prove if Cajanus cajan contain anti-bacterial properties against S.mutans and if filtrating or not the extract will affect in any way the antibacterial potential of the extract.

Hypotesis The Cajanus cajan leaf extract will inhibit the

growth of S.mutans and the filtered extract will have a better inhibitory effect than the unfiltered extract.

MATHERIALS AND METHODS

Grind the leaves.Obtain extract by adding water and processing it in a blender

Vacuum filtration of the extract.

Carry out aseptic techniques.

Make a bacterial lawn with S. mutans

Place the steril filter paper disc.Incubate the Petri dishes.

RESULTS

Measurements in mm

Trial 1 Trial 2 Trial 3

Cajanus cajan leaf extract (unfiltered)

0.0 0.0 0.0

Cajanus cajan leaf extract (filtered)

1.3 1.8 4.3

Control 0.0 0.9 0.0

Table 1: Inhibition zone measurements

Figure 1: Inhibition zone measurements

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

Trial 1 Trial 2 Trial 3

Size

of

inhi

biti

on z

ones

(mm

)

Experimentation Trails

Leaf extract (unfiltered)

Leaf extract (filtered)

Control

REFERENCES BioWeb [Internet]. 2002. (WI):U.S.A. University of

Wisconsin System universities and centers. [cited Oct 15 2011]. Available in: http://bioweb.uwlax.edu/bio203/s2007/allmann_ambe/

Ezeifeka, G.O., Orji, M.U., Mbata, T.I., Patrick, A.O. 2004. Antimicrobial Activities of Cajanus cajan , Garcinia Kola and Xylopia aethiopica on Patogenic Microorganism. Biotechnology [Internet]. [cited 2011 Oct 15]; 3(1): 41-43. Available in. Google Academics.

Kong, Y., Fu, Y., Zu, Y., Chang, F., Chen, Y., Liu, X., Stelten, J., Schiebel, H. 2010. Cajanuslactone, a new coumarin with anti-bacterial activity from pigeon pea [Cajanus cajan (L.) Millsp.] leaves. Food Chem [Internet]. [cited 2011 Oct 15]; 121(4): 1150-1155. Available in: Science Direct.

Raintree Nutrition [Internet]. 2005. Carson City (NV): Raintree Nutrition, Inc. [updated 2010 Mar 20; cited 2011 Oct 15]. Available in: http://www.rain-tree.com/guandu.htm

  United States Department of Agriculture [Internet]. 2011.

Washington (D.C): Natural Resources Conservation Services.[ 2011 Oct 15]. Available in: http://plants.usda.gov/java/profile?symbol=CACA27

   Wu, N., Kong, Y., Fu, Y., Zu, Y., Yang, Z., Yand, M., Peng, X.,

Efferth, T. 2010. In Vitro Antioxidant Properties, DNA Damage Protective Activity, and Xanthine Oxidase Inhibitory Effect of Cajaninstilbene Acid, a Stilbene Compound Derived from Pigeon Pea [Cajanus cajan (L.) Millsp.] Leaves. J. Agric. Food Chem. [Internet]. [cited 2011 Oct 15]; 59(1): 437-443. Available in: ACS Chemistry for Life.

Zu, Y., Liu, X., Fu, Y., Wu, N., Kong, Y., Wink, M. 2010. Chemical composition of the SFE-CO2 extracts from Cajanus cajan (L.) Huth and their antimicrobial activity in vitro and in vivo. Phytomedicine [Internet]. [cited 2011 Oct 15]; 17(14): 1095-1101. Available in: Science Direct.