ANNUAL REPORT - DEPARTMENT OIi' PHYSIOLOGYUNIVERSIT Y OF KANSAS MEDICAL CENTER

July I, 1965 - June 30, 1966

ACADEMIC STAFF

E. B. Brown, Jr., Ph. D., Professor and ChairmanAlan M. Thompson, Ph. D., Associate ProfessorNorman Loofbourrow, Ph. D., Associate ProfessorLawrence Sullivan, Ph. D., Associate ProfessorDonald Johnson, Ph. D., Associate Professor (Joint appointment, Ob-Gyn)Kenneth Goetz, Ph. D., Assistant ProfessorJohn Trank, Ph. D., Assistant ProfessorA. Stark Wolkoff, M. D., Assistant Professor (Joint appointment, Ob-Gyn)

POSTDOCTORAL FELLOWS

Joseph McMullen, M. D., Department of SurgeryKeith Ashcraft, M. D., Department of SurgeryTakuma Hojo, M. D., Medical Fellow. Dept. of Surgery-PhysiologyDarren Nelson, Ph. D., Ob-Gyn Fellow with Dr. JohnsonTomi-O Hishida, D. Sc., Nagoya University, Fellow with Dr. JohnsonWoo Gyeum Kim, M. D., (International Atomic Energy Agency Fellowship),

Seoul National University, KoreaNorberto Gonzalez, M. D., (Postdoctoral fellowship, Nat. Res. Council,

Argentina)Marjorie Gerbrandt, M. D., CV Training qrant

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Lilly KungRay CookLarry WeUingPaul HensleighRuth McDanielConrad JohansonAaron Davis

Barbara McDougalMartin Kramen

Dennis WaringGary Bond

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KA W VALLEY HEART STUDENTS (The Science Talent Search Program,summer 1965)

Conice LaddDelores Clawson

Annual Report, Dept. Physio!.7/1/65 - 6/30/66Page 2

MEDICAL STUDENTS - (Summer Research Program)

Sam Steele

Bob EndbergHugh DickTed GnauWm. White

Garry WeaverClio RobertsonVictor VorheesBob GibbonsBob StephensonGordon EllerIra CoxRichard MorrisonCleve HowardDavid Sneid (Res. Trainee on CV Research Training Grant)

TEACHING:

The medical school curriculum for freshman students was changed so thatGross Anatomy, Histology, and Neuroanatomy were taught in the fall quarter.Physiology and Biochemistry were taught during the Winter and Spring quarter s,except for the last two weeks of the spring quarter when Endocrinology wastaught. French Reading A was taught for the first time here on this campusbeginning June 13. This is an eight-weeks' course, 4-1/2 qtr. hrs. credit,taught by Miss Devlin two days a week, Monday 9 to 12, VIednesday 9 to 11.

Graduate Courses taught during the past year were as follows:Fall: Course #322, Physiology of the Nervous System (3 cr. ),Winter: Course #310 - Transport and Permeability (2 cr. ) Dr.Spring: Course #315 - Renal Electrolyte Excretion (2 cr. ) Dr.

Dr. Loofbourrow

ThompsonSullivan

Graduate Seminars #351 offered during the year were:Fall: Dr. Sullivan and Mr. Robt. Pendleton offered "Ion Transport Across

Epithelial Cell Layers. 11

Winter: Seminar on Neurophysiology, Dr. Loofbourrow.Winter: Dr. Brown's seminar on Regulation of Pulmonary Ventilation.Spring: Topics in Biophysics - Dr. Trank

The integrated course in "Basic Human Biology" for nurses was againtaught in the summer of 1965 by the combined departments of Biochemistry,Anatomy, and Physiology. Drs. Goetz and Johnson were the Senior Staffmembers from this department participating in this course. Medical studentsStan Mosier, John Hiebert, Robert Alberti, and William George assisted inthe course.

Annual Report, Dept. Physio!..7/1/65 - 6/30/66Page 3

Faculty members participated in correlation course for Freshmenmedical students on Saturday morning. Doctors Brown, Goetz, Loofbourrowand Thompson gave presentations on their research to students and teachersfrom colleges in the Kansas City Regional Council on two Saturdays inFebruary and March.

New additions to this department's staff for the coming year areDrs. Goodridge and Rhodes.

In addition to the senior staff members, Dr. Virginia Tucker was responsiblefor a conference section of one group of students in the medical school physiologycourse. Postdoctoral fellows Kim, Gerbrandt, and Gonzalez also assisted with

teaching this course, together with graduate students D. Ray Cook and Lilly Kung.

Mr. Dale Mayhew, who completed work for the M. A. degree in June 1965,received his degree this year (1966). Miss Lilly Kung completed work for theM. A. \ ~degree in this department on June 3, 1966. Mr. Robert Pendleton com-pleted work for the Ph. D. degree this spring and he received the degree in June1966. Mr. Pendleton was a graduate student in Pharmacology, although his thesiswork was carried out in the Department of Physiology under Dr. L. P. Sullivan.Miss Kung is going home to San Francisco for the summer and will take a 12-weekcourse for work in the Peace Corps and expects to be sent to Ghana with othersof the Peace Corps in September. Dr. Pendleton is now employed by Smith,Kline, and French Laboratories in Philadelphia, Pennsylvania.

Many staff members of other departments here at KUMC gave PhysiologyDepartment Seminars.

VISITORS AND OUTSIDE SPEAKERS:

The following visiting professors presented lectures during the year:

10/18/65 Dr. Ralph Kitchell, Dean of Veterinary Med., Ks. St. Univ."Taste in Dogs. "

10/25/65 Dr. Jerome Yochim, Asst. Prof., Dept. Compo Biochem. andPhysiol. University of Kansas at Lawrence. IIHormona1 Controlof the Rat Uterus During the Progestational Phase. II

. 12/6/65 Dr. Fred Grodins, Prof. of Physio!., Northwestern Univ., Chicago,"Regulation of Pulmonary Ventilation. II

12/20/65

1/10/66

1/17 /66

1/24/66

1/31/66

2/17 /66

2/28/66

4/25/66

5/9/66

5/10 / 66

5/16/66

Annual Report. Dept. Physiol.7/1/65 - 6/30/66Page 4

Dr. Roger Fedde) Dept. of Physiol.. Kansas State University."Mechanisms of Resipratory Muscle Activity. "

Dr. H. W. Shirer. Depts. Electrical Engr. and Compo Biochem.and Physiol.) Lawrence) Kansas (Univ. of Kansas) "Biotelemetry."

Dr. M. J. Oppenheimer. ProL and Chrm.. Dept. of Physio1. .Temple University, Philadelphia, Pennsylvania. "Role ofReflexes in Cardiogenic Shock. "

Dr. Alan Goodridge, Dept. Biological Chern., Harvard Med. Sch..Boston) Massachusetts. 'ILipogenesis in the Pigeon. "

Dr. Otto H. Schmitt, Prof. Physics and Zoology, Univ. of Minnesota.Efforts toward making Vector-Electrocardiography Quantitative. "

Dr. L. T. Rutledge, Dept. Physiology, Univ. of Michigan, Ann Arbor.'IAssociative Cortex: Converging input and integrative functions. "

Dr. Nathan Lifson, Dept. Physiol., Univ. Minnesota, presented twoseminars. "A Simple Diffusive-Convective model for IntestinalAbsorption of Nonelectrolytestr was given as part of Dr. Thompson'sTransport Course, and an open lecture on " Reconstruction of FluidCirculation Theory for Intestinal Absorption. "

Dr. Paul H. Kopper, Chrm. Dept. BioI., Washburn University ofTopeka, Kansas. "Gluconate as an activator of blood clot lysisin vivo. II

Dr. Richard L. Malvin, Dept. of Physiol., Univ. of Michigan."Renal Disease as Simulated by Intra-arterial Injections ofMicrospheres. II

Dr. Malvin also talked on "Effect of Blood and Tubular Fluid FlowRates on the Renal Countercurrent Mechanism. "

Dr. Nancy A. Dahl, Department of Compo Biochem. and Physiol.University of Kansas. Lawrence, Kansas. "A Neuron's Use ofEnergy. "

Annual Report-Dept. Physio1.7/1/65 to 6/30/66Page 5

ACTIVITIES OF STAFF:

Dr. E. B. Brown, Jr., Professor and Chairman

Editor: Proc. Soc. Exp. Biol. Med.Modern Medicine

Member Am. Physio!. Soc. Finance Committee.Kaw Valley Heart Board Member.

Taught a course on Acid-Base Balance (9 lectures) to Dr. James E1am'sgroup in the Department of Anesthesiology, General Hospital, Kansas City,Missouri. Lectured to residents and staff of Department of Anesthesiologyat KUMC (5 lectures). Presented a paper at the xxm InternationalCongress of Physiological Sciences in Tokyo, Japan, September 1965.

Presented a paper at the IIConference on Potassium and the Heartll inLexington, Kentucky, October 1-2, 1965.

Lectured at the invitation of Dr. Daniel Simmons October 24-30, 1965,

in Los Angeles as follows:Mt. Sinai Hospital Staff, IIRespiratory Acidosis. II

Cedars of Lebanon Hospital Renal Conference, IIPotassiumMovement and the Donnan Equi1ibrium~'UCLA Physiology Dept., Cardio-respiratory seminar. IIC02buffering of blood and tissues. II

On 11-17 -65 lectured at Emporia Junior High School to approximately200 high school science students and their teachers. Subject: IIHowthe body gets its oxygen. II

Gave seminar to Biology Department students at Ottawa, University,in December.

Dr. J. D. Nelms from the University College of Rhodesia and Nyasaland,Salisbury, Rhodesia, South Africa, was hosted by Dr. Brown of thisDepartment while Dr. Nelms visited here learning our techniques inteaching physiology (Jan. 25-31, 1966).

Offered an Elective Course #217 on Saturday mornings from 2/19/66 to3/19/66, on IIPhysiology and Biochemistry of Acid-Base Balance. II

Was the lIexternal examinerll for the Ph. D. degree for graduate studentKen Ackles, and lectured to staff and graduate students of the Departmentof Physiology, University of Alberta, Edmonton, Alberta, Canada, inFebruary. Subject: "Potassium Transport and the Donnan Equilibrium. II

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Annual Report.Dept. Physiol.7/1/65 - 6/30/66Page 6

Dr. Brown was part of a site inspection group for NIH Grants Division(Drs. Roggensack of NIH and Hertzman of St. Louis) to visit theUniversity of South Dakota, Department of Physiology (Vermillion)Sioux Falls, South Dakota.

Lecture to Students at Kansas State University, Manhattan, Kansas,on May 4 at the invitation of Dr. Roger Fedde.

To Groton, Connecticut, to attend a Symposium sponsored by MedicalEducation for National Defense. Topic of meeting: '!Beneath the Sea. II

Presented a paper at the 9th Emphysema Conference, Aspen, Colorado.,June 8-12, 1966.

Presented a paper at FASEB meeting April 11-14, 1966, in Atlantic City,New Jersey. Title: Increase in extracellular potassium concentrationfollowing expansion of extracellular volume.

Dr. Alan M. Thompson

Review Editor for papers submitted to Science magazine.Member of KUMC Animal Care Committee.

Coauthor of paper presented by Clio Robertson, 3rd yr. medicalstudent at Fall Meeting of APS in Los Angeles on a rat head perfusiontechnique. Thomas A. Bauer, 3rd yr. student, was also an author.Gave a seminar to the Department of Comparative Biochemistry andPhysiology at Lawrence in January on Barium-soluble tissue CO2'Sponsor of R. Clio Robertson, 3rd year student, for a paper

given at KUMC Research Day, March 23, 1966, on Brain Tissue CO2:Effect of Diamox and Hypercapnia.Attended FASEB meetings Aprilll-l6, 1966, and presented paper on

Brain tissue CO2 studies in the perfused rat head.Lectured on Cerebral Blood Flow to Neurosurgery Course at KUMC.Attended MEND symposium in Groton, Connecticut, in May onunderwater physiology.

Dr. Norman Loofbourrow

Was again active in the Neurology Section of the Department of Medicine,Division of Neurosurgery, and the Basic Science Departments, and gavea seminar on the Limbic System, May 19, 1966.

Principal advisor to Lilly Kung during work on her M. A. degree whichwas completed in June 1966.

Also continues to read manuscripts for Dr. E. Gellhorn, ProfessorEmeritus of Neurophysiology, University of Minnesota.

Annual Report - Dept. Physiol.7/1/65 - 6/30/66Page 7

Dr. L. P. Sullivan

Principal advisor for Robert Pendleton who completed his work for thePh. D. in Pharmacology. In addition, Dale Mayhew who completed hiswork for a M. S. degree in Physiology in June 1965 received his degreethis year.

Edited paper s for Journal of Pharmacology and the American Journalof Physiology.

Seminar speaker on the Lawrence Campus, University of Kansas,Department of Comparative Biochemistry and Physiology, Fall of 1965.

Attended meeting of American Physiological Society in Los Angeles,September 1965. Mr. Robert Pendleton presented a paper at thatmeeting covering work done by Mr. Pendleton and Dr. Sullivan.

Attended meetings of FASEB in April 1966.

Attended NYAS symposium f1Physiology of Diuretic Agents" in March,

Participated in clinical correlation conference for first year students.

Member of KUMC Research Committee

Member of University Committee on physical and chemical biology(one of the science development committees).

Promoted to Associate Professor, July I, 1965.

1966.

Began first year of career development award, July 1, 1965.

Dr. Donald C. Johnson

May 12 to June 1, Dr. Johnson attended meetings in Venice, Italy, andin Milan, Italy. The Venice conference sponsored by the Ford FoundationPopulation Program was on the Physiology of Human Reproduction. andthe 2nd International Congress of Hormonal Steroids was held in Milan.

Presented a lecture to Department of Zoology, Kansas State University,March 24, 1966, on "Role of Hormones in differentiation of physiologicsex."

To Chicago 6/20/66 attending meetings of Endocrinology Society.Editor: Endocrinology

Participated in a joint program in Reproductive Physiology with variousdepartments at Kansas State University and the Lawrence Campus whichstarted in the summer of 1965. In October 1965 Kansas State hosted the

group (Animal and Dairy Science). InFebruary 1966, the Department of

Annual Report-Dept. Physiol.7/1/65 to 6/30/66Page 8

Comparative Biochemistry and Physiology at Lawrence presented aprogram, while in June 1966 KUMC was again host. This programhas been a great success. In each meeting about 40 to 50 studentsand staff have been present for two hours of lectures and discussionas well as a joint luncheon.

Dr. Kenneth L. Goetz

Attended the fall meetings of the American Physiological Society in LosAngeles. California, August 23-27, 1965.

In July and August 1965 Dr. Goetz gave a series of nine lectures tostaff at Osawatomie State Hospital.

Attended the FASEB meetings in Atlantic City. New Jersey in April 1966.

Was the speaker at a meeting of the Cardiovascular Research group onMay 10. 1966. Topic: Recording cardiovascular data from trained,unanesthetized dogs.

Dr. John W. Trank

Joined our staff August 1, 1964.

Gave a talk at Midwest Research Institute at Dr. Goodson's invitationon 7/15/65.

Attended the 18th Annual Conference on Engineering in Medicine andBiology, Philadelphia, Pennsylvania, November 10-12, 1965.

Lectured to Bioinstrumentation course at University of Kansas.Topic: Cardiovascular control. II

Presented seminar at Kansas State University, Departrrent of Physiology,Manhattan, Kansas. Topic: 11Central nervous representation ofcardiova scular control. "

Lectured to Neurosurgeons on If Basic electricity and nerve membraneexcitability. I!

Acted as consultant in Biomedical engineering to NASA and MidwestResearch Institute.

Dr. A. Stark Wolkoff

Lectured to American College of Obstetricians and Gynecologists on"Effects of Anesthesia on the unborn fetus'1 at Gatlinburg, Tennessee.October 6, 1965.

Participant at the Third International Trophoblast Conference,Rochester, New York, November 1-3, 1965.

..",,'

Annual Report-Dept. Physio!.7/1/65 - 6/30/66Page 9

Prepared and conducted program of Obstetrical and GynecologicalTravel Club at the University of Kansas Medical Center, November5, 1965.

Lectured on "Endotoxic shock" to Golden Belt Medical Society,Salina, Kansas, November 18. 1965.

Official guest, South Atlantic Association of Obstetrics and Gynecology,Miami. Florida, January 26-29, 1966.

Attended Society for Gynecologic Investigation, San Francisco,March 24-25, 1966.

Conducted two luncheon meetings on "Current research on the sheepfetus" at the American College of Obstetricians and Gynecologistsmeeting in Chicago. illinois, May 2 -3. 1966.

Lecture on "Fetal physiology'! and lectures on :tDigestion" May 1966.

Supervised animal lab for Group B freshman physiology all dayWednesday during winter and spring quarter s.

Participated in tutorial seminars Tuesdays at 1:00 for Group Bwinter and spring quarter s.

RESEARCHResearch grants which were active during the year were the following:

Intracellular Compensatory Changes in Response to Chronic RespiratoryAcidosis and Alkalosis. Air Force Contract #AF 41 (609)-3005, USAF

School of Aerospace Medicine, Brooks Air Force Base, Texas. PrincipalInvestigator: E. B. Brown. Jr. Jan. 1,1966 to Dec. 31, 1966. $18,662.00

Potassium Metabolism and Acid-Base Balance. USPHS #5 R01 AM 05649.

Principal Investigator: E. B. Brown, Jr. June 1. 1965 to May 31, 1966.Extended without additional funds to November 30, 1966. $13, 500 -Supplement for equipment purchase $13,500.

Tissue CO2 Chemical Forms and Exchange Rate. USPHS #GM-10429.Principal Investigator: Alan M. Thompson. Sept. 1. 1964, - August31. 1965. Extended to August 31, 1966, without additional funds. $14.400.

Control of Collecting Duct Secretion of Hydrogen and Potassium.National Science Foundation #GB-655. Principal Investigator: LawrenceP. Sullivan. Jan. 1. 1965 - Dec. 31, 1965. $16. 900.

Factors Controlling Renal Excretion of Ammonium. Hydrogen andPotassium. National Science Foundation. #GB-4314. PrincipalInvestigator: Lawrence P. Sullivan. Jan. 1, 1966 - Dec. 31. 1967.$35,400 (for 2 years).

Annual Report-Dept. Physiol.7/1/65 - 6/30/66Page 10

Hydrogen Ion Retention in Diabetic Acidosis and Ketosis. USPHS#AM-06570. Principal Investigator: Virginia Tucker. Co-principalInvestigator: E. B. Brown, Jr. Sept. 1, 1965 - Aug. 31, 1966. $10,860.

Regulatory Mechanisms of Blood Flow. USPHS #HE-08609. PrincipalInvestigator: C. F. Kittle. Co-principal Investigator: E. B. Brown, Jr.March 1, 1965 - Feb. 28, 1966, $l8,391. Renewal of above.. March 1, 1966- to be transferred when Dr. Kittle leaves KUMC. $20,280. 00.

Right Atrial Pressure and Heart Rate. USPHS #1 R01 HE-08803.Principal Investigator: K. L. Goetz. June 1, 1965 - May 31, 1966,$13, 320. 00.

Motor Effects of the Arrest Reaction. USPHS #MH 08908. PrincipalInvestigator: G. N. Loofbourrow. June 1, 1965 -May 31, 1966.$13,678.00. Extended to November 31, 1966 without additional funds.

Studies on the Maternal-Fetal Relationship in Sheep. USPHS #HD-00556.Principal Investigator: A. Stark Wolkoff. Jan. 1, 1965 -Dec. 31, 1965.$11,000. Jan. 1, 1966 -Dec. 31, 1966. $13,053.00.

PHS Training Grant. Program Director: E. B. Brown, Jr. #5T1GM1137-02. July 1, 1965 - June 30, 1966. $36,000.00.

General Research Support Grant, allocated to Department of Physiology.Jan. 1, 1965 - Dec. 31, 1965. $3, 000. Inst. #05890450.

Life Insurance Medical Research Fund. KU Endowment Association.#64R1327. Principal Investigator: John W. Trank. $9,000.July 7, 1964 - June 30, 1966.

Fellowship Award: U, S. ~blic Health #1-GI-GM-20, 339..01. PrincipalInvestigator: Alan M. Thompson. Fellow: Robert E. Thurber.June 1, 1963 - depletion of grant. $7,000. 00. Now terminated.

Fellowship Award, Public Health #5-FI-GM-13, 199-04. PrincipalInvestigator: E. B. Brown, Jr. Fellow: Richard L. Clancy. Jan. I,1964 - depletion of grant. $5,700. Now terminated.

Diuretic Agents and Ion Transport in the Toad Bladder. 1ROI AM 10227,USPHS. Principal Investigator: Lawrence P. Sullivan. $15, 948.Jan. 1, 1966 - Dec. 31, 1966.

Annual Report-Dept. Physiol.7/1/65 - 6/30/66Page 11

The Correlation of Electrical Activity with Cardiovascular State. KUMCInstitutional Grant #09589094. Principal Investigator: John W. Trank.$2,920. Oct. 1, 1965 - Sept. 30, 1966.

Kansas Heart Association. In vivo cardiac tissue buffering. PrincipalInvestigator: E. B. Brown. Jr. $5,000.00. July 1, 1965 - June 30, 1966.

Kansas Heart Association. Factors controlling the renal excretion ofhydrogen and potassium. Principal Investigator: L. P. Sullivan.$3,595.00. July 1, 1965 - June 30, 1966.

Career Development Award #5-K3-GM-ll, 898-01. Renal TubularPotassium Transport. Dr. Brown in behalf of L. P. Sullivan. $14,568. 00.

Kansas Heart Association. Coronary blood flow as related to right heartpressure. Principal Investigator: K. L. Goetz. $4,558.00. July 1, 1965 -June 30, 1966.

PUBLICA TIONS

Brown, E. B., Jr. and R. L. Clancy. In vivo and in vitro CO2 bloodbuffer curves. J. Appl. Physiol. 20:No.5, 885-889, 1965.

Brown, E. B., Jr. Blood a.nd tissue buffers. Arch. Intern. Med.116:665-669, 1965. (Symposium on Acid-Base Balance, Nov. 1965).

Brown, E. B., Jr. Detection and quantitation of metabolic acidosisand alkalosis. Annals N. Y. Acad. Sc. 133:118-124,1965.

Brown, E. B., Jr. Acid-Base Debate.Anesthesiology 27:333, 1966.

(Letter to the Editor)

Brown, E. B., Jr., R. L. Clancy, W. G. Kim, and Virginia Tucker.Quantitative aspects of CO2 buffering of tissue. Am. Rev. of Resp.Diseases (In press). (9th Emphysema Conference, Aspen, Colorado,June 8-12, 1966.)

Brown, E. B., Jr., R. L. Clancy, and W. G. Kim. PotassiumMovement and intracellular-extracellular hydrogen ion ratio changes.Proc. xxm International Congress of Physiol. Scs., Tokyo. Japan,September 1-9, 1965. (Abstract)

Brown, E. B., Jr. and W. G. Kim. Increase in extracellular potassiumconcentration following expansion of extracellular volume. Fed. Proc.25:631, 1966. (Abstract)

""

Annual Report-Dept. Physio1.7/1/65-6/30/66Page 12

Robertson. R. C.. T. A. Bauer and A. M. Thompson. A"rat headperfusion technique developed for the study of brain uptake of materials.Physiologist 8:261. 1965.

Pekas. J. C., A. M. Thompson and V. W. Hays. Characteristics ofthe exocrine pancreatic secretion of the young pig. J. Anim. Sci.25:113, 1966.

Thompson, A. M., R. C. Robertson and T. A. Bauer. Brain CO2 studiesin the perfused rat head: Effect of acetazolamide and hypercapnia.Fed. Proc. 25:512., 1966.

Goetz. Kenneth L. Effect of increased pressure within a right heartcul-de-sac upon heart rate in dogs. Am. J. Physiol. 209:507-512. 1965.

Marian C. Kuenzig. Robert W. Hamilton. Jr. and Leonard F. Peltier.Dipa1mitoy11ecithin: studies on surface properties. J. Appl. Physio!.20:779-782.1965.

Dr. G. N. Loofbourrow - Japanese and Russian translations have recentlybecome available of EMOTIONS and EMOTIONAL DISORDERS - a Neuro-Physiological Study (Ernst Gellhorn and G. N. Loofbourrow, Hoeber. 1963.)

Schmidt. Ramon W. and L. P. Sullivan. Effect of Meralluride on distalnephron transport of sodium, potassium and chloride. J. Pharmacol.151:180-188,1966.

Sullivan, L. P. and J. H. Pirch. Effect of Bendroflumethiazide on distalnephron transport of sodium. potassium and chloride. J. Pharmacol.151:168-179.1966.

Sullivan, L. P. Ammonium excretion during stopped flow: a hypotheticalammonium countercurrent system. Am. J. Physio1. 209:273-282.1965.

Pendleton, R. B. and L. P. Sullivan. The effect of Bendroflumethiazideupon sodium transport by the isolated toad bladder. Physiologist.8:250. Aug. 1965. (Abstract)

Johnson. D. C. The use of non-castrate parabiotic rats for the evaluationof plasma gonadotrophins. Acta Endocrino1ogica. 51:(2)269-280, (1966).

Annual Report-Dept. Physiol.7/1/65-6/30/66Page 13

Johnson, D. C., and D. M. Nelson. Temporal aspects of pituitarylutEinizing hormone (LH) synthesis in the rat receiving sheep hypothalamicextract. Physiologist 8:(3),2.01,1965.

Johnson, D. C., and D. M. Nelson. The amount of lutEinizing hormoneactivity in extracts of sheep hypothalamus. Endocrinology 78:901-903(1966).

Foltz, F. M., D. C. Johnson, and D. M. Nelson. Methods for obtainingvenous outflow from the hypothalamus and hypophysis. Proc. Soc. Exp.BioI. Med. 122.:22.3-22.7(1966).

Johnson, D. C.. and D. M. Nelson. Rapid changes in hypophysiallutenizing hormone content in immature pseudopregnant rats. Programof the Forty-eighth meeting of Endocrine Society (Chicago, nl.. June 1966)p. 122.

Morrison, R. L. and D. C. Johnson. The effects of Androgenizationin male rats castrated at birth. J. Endocrin. (1966), 34,117-123.

Lauer, R. M., J. A. Evans, H. Aoki, and C. F. Kittle. Factorscontrolling pulmonary vascular resistance in fetal lambs. J. Pediatrics,67:(4)568-577,1965.

Wolkoff, A. S., Bawden, J. W., Flowers, C. E.. Jr. and McGee, J. A.The effects of anesthesia in the unborn fetus. Am. J. Ob-Gyn. 93:311,1965.

Wolkoff, A. S. and Arnold. P. G. Experimental lung lesions in theunborn fetus. Kansas Med. J., to be published.

Bawden. J. W. a.nd Wolkoff, A. S. Fetal blood calcium responses tomaternal calcium perfusion in sheep. To be published.