Annual Meeting ISEV2016 - cdn.ymaws.com · Poster Session 1 - Cell Biology of EVs: Biogenesis and...

Annual Meeting — ISEV2016Rotterdam, The Netherlands

4–7 May 2016

www.isev.org

Annual Meeting — ISEV2016 | Rotterdam, The Netherlands | 4–7 May 2016 2

Annual Meeting — ISEV2016 | Rotterdam, The Netherlands | 4–7 May 20163

1st ProgramNovel Developments of Isolation and Characterization of Extracellular Vesicles

8:15 AM Registration9:00 AM Welcome9:05 AM Massive Online Open Course MOOC - Basics of extracellular vesicles

Speaker: Cecilia Lässer

Introduction to EVs and EV Isolation9:15 AM Heterogeneity and preanalytical factors

Speaker: Jan Lötvall 9:35 AM Isolation techniques - comparative study

Speaker: An Hendrix9:55 AM Summary of the results of the ISEV survey

Speaker: Chris Gardiner

EV Imaging10:10 AM Transmission electron microscopy of EVs

Speaker: Guillaume van Niel 10:25 AM Cryo electron microscopy of EVs

Speaker: Alain Brisson 10:40 AM Visualization and tracking of EVs

Speaker: Charles Lai 10:55 AM Break

High Throughput Analysis of EVs11:10 AM NTA analysis of EVs

Speaker: Chris Gardiner11:25 AM Tunable resistive pulse sensing analysis of EVs

Speaker: Edwin van der Pol11:40 AM Flow cytometry of EVs

Speaker: Marca Wauben11:55 AM Comparison of NTA/qNano/high resolution flow cytometry

Speaker: Esther Nolte-‘t Hoen12:10 PM Lunch

Novel Developments in EV Isolation1:10 PM Aqueous two phase system

Speaker: Jaesung Park1:25 PM Heparin affinity isolation of EVs

Speaker: Leonora Balaj1:40 PM Synthetic peptide-based affinity isolation of EVs

Speaker: Anirban Ghosh

Novel Developments of EV Analysis1:55 PM Surface plasmon resonance analysis of EVs

Speaker: Fredrik Höök 2:10 PM Raman tweezers nanospectroscopy of EVs

Speaker: Sergei Kruglik 2:25 PM Break

OMICS Technologies2:55 PM Transcriptomics of EVs

Speaker: Andrew Hill3:10 PM Integrative proteogenomics and shotgun lipidomics of EVs

Speaker: Suresh Mathivanan3:25 PM Metabolomics of EVs

Speaker: Juan Manuel Falcon-Perez

EV Enrichment in Clinical Settings3:40 PM Lipid determination of EV preparations

Speaker: Edit I Buzás3:55 PM Size exclusion chromatography of EVs

Speaker: Rienk Nieuwland4:10 PM Purification of EVs using ultrafiltration and subsequent size

exclusion chromatographySpeaker: Fiona Lee

4:25 PM Hydrostatic dialysis of EVsSpeaker: Harry Holthofer

4:40 PM Concluding Remarks: María Yáñez-Mó

TUESDAY, 3 MAY 2016PRE-MEETING EDUCATION DAY PROGRAM

2nd ProgramExtracellular Vesicle Therapeutics

8:15 AM Registration9:00 AM Welcome9:05 AM Massive Online Open Course MOOC - Basics of extracellular vesicles

Speaker: Yong Song Gho

Introduction to EV Therapeutics9:15 AM EV-based therapeutics; general aspects and perspectives

Speaker: Bernd Giebel9:45 AM Manufacturing, safety and regulatory requirements

Speaker: Eva Rohde

EV-based Immunomodulation10:15 AM Immunomodulation by immune cell-derived EVs

Speaker: Paul D Robbins10:30 AM Immunomodulation by tumor cell-derived EVs

Speaker: Michael Graner 10:45 AM Immunomodulation by stem cell-derived EVs

Speaker: Giovanni Camussi11:00 AM Immunomodulation by milk EVs

Speaker: Susanne Gabrielsson11:15 AM Break

Design of EV-based or EV-inspired Carriers11:30 AM Bioinspired exosome-mimetic nanovesicles

Speaker: Yong Song Gho11:45 AM Considerations regarding membrane curvature and targeting

Speaker: Hang Hubert Yin12:00 PM Vexosomes

Speaker: Casey Maguire12:15 PM Artificial bioinspired EVs

Speaker: Raymond Schiffelers12:30 PM Lunch

EV vaccines in Infectious Diseases1:30 PM Challenges of anti-viral EV vaccine development

Speaker: Ken Witwer1:45 PM OMV-based vaccination

Speaker: Oh Youn Kim2:00 PM Vaccination with parasite derived EVs

Speaker: Hernando del Portillo

EV Therapeutics in Clinic2:15 PM EV therapy in cancer

Speaker: Clotilde Théry 2:35 PM EV therapy in neurological diseases

Speaker: Lawrence Rajendran 2:55 PM MSC-derived EV therapy

Speaker: Sai Kiang Lim 3:15 PM Break

EVs in Preclinical Models3:30 PM Cardiac repair after myocardial infarction

Speaker: Susmita Sahoo

Plant EV therapeutics3:45 PM Therapeutic applications of edible plant EVs

Speaker: Riccardo Alessandro4:00 PM Concluding Remarks: Lorraine O’Driscoll


WEDNESDAY, 4 MAY 2016

7:00am – 6:00pm Registration

8:30 – 9:00am Welcome Coffee

9:00 – 9:20am Opening SessionsRoom: Willem Burger

9:20 – 10:30am

Plenary Session 1 – EVs in CancerSpeakers: Klaus Pantel (Germany) and David Lyden (USA)

Chairs: Clotilde Théry and Pascale ZimmermannRoom: Willem Burger

10:30 – 11:00am Networking Coffee

11:00 – 12:30pmSymposium Session 2

EV Protemics and LipidomicsRoom: van Weelde

Symposium Session 1EVs and Implications to Cancer Therapy

Room: Willem Burger

Symposium Session 3Cell Biology and EVs I

Room: Jurriaanse

12:30 – 1:30pm Lunch

1:30 – 2:15pmOral with Poster Session 2

EVs, Immunity and More Room: van Weelde

Oral with Poster Session 1EV Isolation, Characterization and Stem Cells

Room: Willem Burger

Oral with Poster Session 3EVs in Cancer and Other Diseases

Room: Jurriaanse


EVs of the CNS Room: van Weelde

Symposium Session 6EVs in Cancer Transcriptomics

Room: Willem Burger

Symposium Session 5EV Isolation

Room: Jurriaanse

3:00 – 3:30pm Networking Coffee

3:30 – 4:00pm

Biotech Sponsored Session 1 (3:30-3:45pm)

Caris Life SciencesRoom: van Weelde Symposium Session 8

(3:30 – 5:00pm)EVs and Tumor Environment I

Room: Willem Burger

Biotech Sponsored Session 2 (3:30-4:00pm)

IZONRoom: Jurriaanse

Symposium Session 7(3:45 – 5:00pm)

EVs in Metabolic Syndrome, Fatty Liver Disease and Cancer

Room: van Weelde

Symposium Session 9(4:00 – 5:00pm)

EV Biomarkers for Cancer IRoom: Jurriaanse

5:00 – 8:00pm

Poster Session 1 - Cell Biology of EVs: Biogenesis and Transfer Poster Session 2 - EVs in Tumor MetastasisPoster Session 3 - Analysis of EVs in Body Fluids Poster Session 4 - EVs as Drug Delivery System for Antitumoral Therapies & VaccinationPoster Session 5 - EVs in Acute and Chronic InflammationPoster Session 6 - EV Proteomics & LipidomicsPoster Session 7 - EV IsolationPoster Session 8 - EVs in Prostate Cancer

6:30 – 8:00pmSatellite

Meet the National and International SocietiesRoom: Jurriaanse


THURSDAY, 5 MAY 2016


8:00 – 8:45am

Meet the Experts Session 3Extracellular Vesicles in

Infectious DiseaseRoom: van Weelde

Meet the Experts Session 1Extracellular Vesicle Heterogeneity: Can we Overcome the Confusion?

Room: Willem Burger

Meet the Experts Session 2Extracellular Vesicles in Regenerative Medicine

Room: Jurriaanse

9:00 – 10:15amSymposium Session 12

EVs in Cardiovascular DiseaseRoom: van Weelde

Symposium Session 11EV Characterization: Flow Cytometry

Room: Willem Burger

Symposium Session 10EVs in Cross-species Communication

Room: Jurriaanse


10:45 – 12:30pm

Plenary Session 2 – Learning from the Best: Viruses and EVsSpeakers: Robert Gallo and Leonid Margolis

Chairs and introduction: Esther Nolte-'t Hoen and Jennifer JonesRoom: Willem Burger

12:30 – 1:30pm Lunch


EVs in Remodeling and RepairRoom: van Weelde

Symposium Session 15EVs and Tumor Environment II

Room: Willem Burger

Symposium Session 14Engineering EVs

Room: Jurriaanse



New Techniques for EV CharacterizationRoom: van Weelde

Symposium Session 18EVs in NeuropathiesRoom: Willem Burger

Symposium Session 17EV Biomarkers for Cancer II

Room: Jurriaanse

5:00 – 8:00pm

Poster Session 1 - EVs, Microbes & Cross-species Communication

Poster Session 2 - EVs in Cardiovascular Disease

Poster Session 3 - EVs in Cardiovascular Disease and Coagulation

Poster Session 4 - EVs in Stem Cells

Poster Session 5 - EVs as Cancer Biomarkers

Poster Session 6 - EVs in the CNS

Poster Session 7 - EV Characterization from Different Sources or Different Subtypes

Poster Session 8 - Late Breaking Poster Session 1

6:30 – 8:00pmSatellite Event

Satellite meeting ISEV-ISAC-ISTH EV flow cytometry work groupRoom: Jurriaanse

FRIDAY, 6 MAY 2016


8:00 – 8:45amMeet the Experts Session 6

Extracellular RNAs: EVs or not EVsRoom: van Weelde

Meet the Experts Session 4Extracellular Vesicles as Modulators of Drug Resistance and Tumor Metastasis

Room: Willem Burger

Meet the Experts Session 5Extracellular Vesicles-Associated Versus

Soluble Functional Molecules Room: Jurriaanse

9:00 – 10:15amSymposium Session 21

EVs in Reproduction and PregnancyRoom: van Weelde

Symposium Session 20EVs in Cancer Metastasis I

Room: Willem Burger

Symposium Session 19EVs in the Immune System

Room: Jurriaanse


5


10:45 – 11:25am

Plenary Session 3 – EVs in Cell BiologyFrancisco Sanchez-Madrid (Spain)

Chairs: Clotilde Théry and Dirk Michiel PegtelRoom: Willem Burger

11:25 – 12:30pm ISEV General AssemblyRoom: Willem Burger

12:30 – 1:30pm Lunch JEV Editorial Board MeetingRoom: Schadee


EVs in Acute and Chronic Inflammatory DiseaseRoom: van Weelde

Symposium Session 24EVs in Immune Modulation in Bacterial

and Parasitic InfectionsRoom: Willem Burger

Symposium Session 23EVs in Cancer Metastasis II

Room: Jurriaanse


3:15 – 4:15pmDiamond Sponsor Session

Caris Life ServicesRoom: Willem Burger


EVs in Cellular Differentiation and DevelopmentRoom: van Weelde

Symposium Session 27Cell Biology of EVs IIRoom: Willem Burger

Symposium Session 25EVs, Viruses and Viral Infections

Room: Jurriaanse

5:00 – 7:30pm

Poster Session 1 - Non-cancer Biomarkers

Poster Session 2 - EVs and Cancer

Poster Session 3 - EVs and the Immune System

Poster Session 4 - EVs in the Tumor Microenvironment

Poster Session 5 - MSCs & Tissue Regeneration and Morphogenesis

Poster Session 6 - Novel Technical Developments in EV Characterization

Poster Session 7 - Late Breaking Poster Session 2

7:30pm Networking Event (registration required)Location: Laurens Church

SATURDAY, 7 MAY 2016


8:00 – 9:00amExperts Meet Session 2

Biofluids: MilkRoom: van Weelde

Experts Meet Session 1Biofluids: Blood

Room: Willem Burger

Experts Meet Session 3Biofluids: Urine

Room: Ruys, van Rijckevorsel, Plate Zaals

9:00 – 10:00am Late Breaking Sessions 2Room: van Weelde

Late Breaking Sessions 1Room: Willem Burger

Late Breaking Sessions 3Room: Ruys, van Rijckevorsel, Plate Zaals


10:30 – 11:50am

Plenary Session 4 with Featured Abstracts ISEV2016Cecilia Lässer: Introduction of the MOOC “Basics of Extracellular Vesicles”

Featured abstract presenters: Benjamin Scicluna, Hefin Jones and Katharina BeerIntroductions: Guillaume Van Niel, Edit I Buzás, and María Yáñez-Mó

Room: Willem Burger

11:50am – 12:35pm Wrap-Up Sessions, Special Achievement Award and Oral and Poster AwardsRoom: Willem Burger

12:35 - 12:45pm Closing Remarks


Welcome!

Dear Colleagues,

On behalf of the International Organizing Committee it is with great pleasure that we invite you to be part of the 5th Annual Meeting of the International Society for Extracellular Vesicles in Rotterdam, the Netherlands, from May 4th to the 7th 2016. It is our honor to organize this meeting where scientists from around the globe share the latest information on extracellular vesicles.

The International Society for Extracellular Vesicles (ISEV) was funded in 2011 as the authoritative international community for extracellular vesicle (EV) research. It focuses on all aspects of extracellular vesicle investigations. Over the past few years, ISEV meetings have received an increasing number of abstracts due to the outstanding quality of these events.

The scientific program of ISEV2016 includes a broad range of educational and scientific sessions covering the entire spectrum of topics relevant to extracellular vesicle research. For the first time, we have expanded our Pre-Meeting Education day program to parallel sessions on two different topics (“New developments of EV isolation and analysis” and “EV therapeutics”).

ISEV is proud to announce prominent plenary speakers highlighting important aspects of EVs and cancer (Klaus Pantel and David C Lyden), EVs and viruses (Leonid Margolis and Robert C Gallo) and EVs in cell biology (Francisco Sanchez Madrid). Parallel symposium sessions, posters and short oral poster presentations will provide platforms to present the newest developments in the field. Between oral and poster presentations, there will be sufficient time for networking and exhibition visits. In keeping with ISEV tradition, we will have “Meet the experts” sessions to get overviews and discussions on hot topics. The novel interactive ”Experts meet” session on three important body fluids (blood, urine and breast milk) will enable community members to discuss body fluid-specific questions of extracellular vesicle isolation and detection. ISEV2016 will also have an attractive Networking event in St. Lauren Church.

By providing an insight into the most recent unpublished data of extracellular vesicle research, ISEV meetings create unique competitive advantage for both academic and commercial partner attendees. In the De Doelen Conference Center of Rotterdam, during lunches and coffee breaks, there will be ample opportunities for scientific interactions.

ISEV annual meetings are not only characterized by an outstandingly high scientific interest by the attendees in the program but also by a very supportive, friendly atmosphere of the discussions. ISEV annual meetings offer an excellent opportunity for researchers to share their recent findings with the scientific community and also provide an unmatched learning opportunity for those who are new to the field.

These days the use electronic knowledge dissemination and communication offer a historically unique opportunity for rapid development of novel fields such as the one of extracellular vesicles. However, there is also an unprecedented responsibility of our community to control the scientific quality of extracellular vesicle-related data, and maintain high standards in extracellular vesicle research. By giving excellent learning opportunities and hosting constructive discussions of current challenges with peers, ISEV2016 facilitates the development of EV methods, provides visibility of new breakthroughs, introduces novel possibilities and gives boost to further research.

The ISEV community is open to other fields. If you have colleagues from other research areas interested in extracellular vesicles, please ask them to join you and visit ISEV2016.

We are committed to creating a most memorable scientific congress. Through your participation, interest and support, you will highly contribute to the success of the meeting and the advancement of the extracellular vesicle field. We hope to meet you in Rotterdam!

On behalf of the International Organizing Committee,

Edit I Buzás


INTERNATIONAL ORGANIZING COMMITTTEE

Edit I Buzás

Aled Clayton Dolores Di Vizio Juan ManuelFalcon-Perez

Lorraine O’DriscollGuido Jenster Yong Song Gho

Marjolein van Driel Hans van Leeuwen Guillaume van Niel Kenneth W WitwerMarca HM Wauben María Yáñez-Mo

8


TUESDAY, 3 MAY 2016PARALLEL SESSIONS

EDUCATION DAY 1ST SESSIONSNOVEL DEVELOPMENTS OF ISOLATION AND CHARACTERIZATION OF EXTRACELLULAR VESICLES

8:15AMREGISTRATION

9:00AMWELCOME

9:05AMMassive Online Open Course MOOC Speaker: CECILIA LASSER

9:15AMINTRODUCTION TO EVS AND EV ISOLATIONHeterogeneity and preanalytical factors Speaker: JAN LÖTVALL

Isolation techniques - comparative study Speaker: AN HENDRIX

Summary of the results of the ISEV survey Speaker: CHRIS GARDINER

10:10AMEV IMAGINGTransmission electron microscopy of EVs Speaker: GUILLAUME VAN NIEL

Cryo electron microscopy of EVsSpeaker: ALAIN BRISSON

Visualization and tracking of EVs Speaker: CHARLES LAI

10:55AMBREAK

11:10AMHIGH THROUGHPUT ANALYSIS OF EVSNTA analysis of EVsSpeaker: CHRIS GARDINER

Tunable resistive pulse sensing analysis of EVsSpeaker: EDWIN VAN DER POL

Flow cytometry of EVsSpeaker: MARCA WAUBEN

Comparison of NTA/qNano/high resolution flow cytometrySpeaker: ESTER NOLTE-‘T HOEN

12:10PMLUNCH

1:10PMNOVEL DEVELOPMENTS IN EV ISOLATIONAqueous two phase systemSpeaker: JAESUNG PARK

Heparin affinity isolation of EVsSpeaker: LEONORA BALAJ

Synthetic peptide-based affinity isolation of EVsSpeaker: ANIRBAN GHOSH

1:55PMNOVEL DEVELOPMENTS OF EV ANALYSISSurface plasmon resonance analysis of EVsSpeaker: FREDRIK HÖÖK

Raman tweezer spectroscopy of EVsSpeaker: SERGEI KRUGLIK

2:25PMBREAK

2:55PMOMICS TECHNOLOGIESTranscriptomics of EVsSpeaker: ANDREW HILL

Integrative proteogenomics and shotgun lipidomics of EVsSpeaker: SURESH MATHIVANAN

Metabolomics of EVsSpeaker: JUAN MANUEL FALCON-PEREZ

PRE-MEETING EDUCATION DAYThe Pre-meeting Education Day is a successful tradition of the annual ISEV meetings. Focusing on particular topics within the extracellular vesicle field, select experts provide deep insight into certain basic aspects of extracellular vesicles. These educational presentations collectively illuminate burning questions and provide a comprehensive insight into the topic.

9


3:40PMEV ENRICHMENT IN CLINICAL SETTINGSLipid determination of EV preparationsSpeaker: EDIT I BUZÁS

Size exclusion chromatography of EVsSpeaker: RIENK NIEUWLAND

Purification of EVs using ultrafiltration and subsequent size exclusion chromatographySpeaker: FIONA LEE

Hydrostatic dialysis of EVsSpeaker: HARRY HOLTHOFER

4:40PMCONCLUDING REMARKSSpeaker: MARÍA YÁÑEZ-MÓ

EDUCATION DAY 2ND SESSIONSEXTRACELLULAR VESICLE THERAPEUTICS

8:15AMREGISTRATION

9:00AM WELCOME

9:05AM Massive Online Open Course (MOOC) - basics of extracellular vesiclesSpeaker: YONG SONG GHO

9:15AMINTRODUCTION TO EV THERAPEUTICSEV-based therapeutics; general aspects and perspectivesSpeaker: BERND GIEBEL

Manufacturing, safety and regulatory requirementsSpeaker: EVA ROHDE

10:15AMEV-BASED IMMUNOMODULATIONImmunomodulation by immune cell-derived EVsSpeaker: PAUL D ROBBINS

Immunomodulation by tumor EVsSpeaker: MICHAEL GRANER

Immunomodulation by stem cell EVsSpeaker: GIOVANNI CAMUSSI

Immunomodulation by milk EVsSpeaker: SUSANNE GABRIELSSON

11:15AMBREAK

11:30AMDESIGN OF EV-BASED OR EV-INSPIRED CARRIERSBioinspired exosome-mimetic nanovesiclesSpeaker: YONG SONG GHO

Considerations regarding membrane curvature and targetingSpeaker: HANG HUBERT YIN

VexosomesSpeaker: CASEY MAGUIRE

Artificial bioinspired EVsSpeaker: RAYMOND SCHIFFELERS

12:30PMLUNCH

1:30PMEV VACCINES IN INFECTIOUS DISEASESChallenges of anti-viral EV vaccine developmentSpeaker: KEN WITWER

OMV-based vaccinationSpeaker: OH YOUN KIM

Vaccination with parasite derived EVsSpeaker: HERNANDO DEL PORTILLO

2:15PMEV THERAPEUTICS IN CLINICEV therapy in cancerSpeaker: CLOTILDE THÉRY

EV therapy in neurological diseasesSpeaker: LAWRENCE RAJENDRAN

MSC-derived EV therapySpeaker: SAI KIANG LIM

3:15PMBREAK

3:30PMEVS IN PRECLINICAL MODELSCardiac repair after myocardial infarctionSpeaker: SUSMITA SAHOO

3:45PMPLANT EV THERAPEUTICSTherapeutic applications of edible plant EVsSpeaker: RICCARDO ALESSANDRO

4:00PMCONCLUDING REMARKSSpeaker: LORRAINE O’DRISCOLL


7:00AM – 6:00PM REGISTRATION

8:30 – 9:00AM WELCOME COFFEE

9:00 – 9:20AM OPENING SESSIONS

9:20 – 10:30AM PLENARY SESSION 1 Room: Willem Burger EVs and Cancer

Speakers: KLAUS PANTEL M.D. is professor and director of the Department of Tumor Biology at the University Medical Center Hamburg-Eppendorf (UKE) – Liquid Biopsy in Cancer Patients

DAVID C LYDEN M.D. PH.D. is professor at Weill Cornell Medical College – The Systemic Effects of Exosome-Mediated Metastasis

Chairs: Clotilde Théry, Pascale Zimmermann

10:30 – 11:00AM NETWORKING COFFEE

11:00 – 12:30PM ORAL SESSIONSRoom: Willem Burger SYMPOSIUM SESSION 1 EVs and Implications to Cancer Therapy

Speakers: KRISTINA P. FRIIS – To Catch a Ride: Packaging and Delivery of Retrotransposons Through Extracellular Vesicles

RAMESH GUPTA – Milk-Derived Exosomes – a Platform Nanocarrier to Enhance Anti-proliferative, Anti-inflammatory and Anti-cancer Activities of Small Drug Molecules Against Multiple Human Cancers

STEPHANIE BOUKOURIS – Exosomes Transfer Chemotherapeutic Drug Resistance Between Colorectal Cancer Cells

DORIVAL RODRIGUES – JUNIOR – Extracellular Vesicles Regulate Gefitinib Sensitivity in Head and Neck Cancer Cells

REKA HARASZTI – Lipid and Protein Composition Distinguishes Stem Cell EVs From Cancer Cell EVs and Affect Efficiency of EV-Mediated Small RNA Cellular Transfer

PETER QUESENBERRY – Extracellular Vesicle Mediated Reversal of Castrate Resistant Prostate Cancer

Chairs: Takahiro Ochiya, Hans van Leeuwen

Room: van Weelde SYMPOSIUM SESSION 2 EV Protemics and Lipidomics

Speakers: MASOOD KAMALI-MOGHADDAM – Advanced Molecular Tools for Proteomic Analyses and Characterization of Microvesicles

ALLAN STENSBALLE – Proteomics Driven Design of Endothelial Stress Based Protein Array for Disease Prognostics – Applied to Plasma and CSF

BIRKE BENEDIKTER – Proteomic Analysis of Extracellular Vesicles Isolated from Airway Epithelial Cells Using Ultrafiltration Followed by Size Exclusion Chromatography

MARTIJN VAN HERWIJNEN – In-depth Proteomics Analysis of Human Breast Milk-derived Extracellular Vesicles Reveals a Novel Functional Proteome Distinct From Other Milk Components

KWANG PYO KIM – Characterization of Extracellular Vesicles Derived From Hepatitis B Virus Producing Liver Cells

IVANA MILIC – Apoptotic Cell-derived Extracellular Vesicles (ACdEV) – Towards Defining a Structure- function Relationship

Chairs: Aled Clayton, Alissa Weaver

Room: Jurriaanse SYMPOSIUM SESSION 3 Cell Biology and EVs I

Speakers: ANTONELLA BONGIOVANNI – The Role of S-palmitoylation in the Biogenesis of Skeletal Muscle-derived Extracellular Vesicles (EVs) and the Structural Organization of Their Membrane

SARAH STEWART – Unexpected Cytosolic Protein Localisation on the Surface of Extracellular Vesicles

BALÁZS BARTOS – Investigation of the Molecular Mechanism of Cargo Editing in Human PMN derived EVs

MARC RUIZ – YKT6 Regulation of Exosome Release is Mediated by MicroRNAs and Impacts Prognosis in Non-small-cell Lung Cancer

DIONYSIOS C. WATSON – Scavenger Receptor A Blockade Leads to Tumor Accumulation of Systemically Administered Extracellular Vesicles Bearing Interleukin-15

UMA ARASU – Binding of EVs is Regulated by CD44-hyaluronan Interactions and Induce EMT-like Changes in Target Cells

Chairs: Michael Graner, Matias Ostrowski

12:30 – 1:30PM LUNCH

ORAL WITH POSTER SESSIONS

ISEV2016 ANNUAL MEETING

11

WEDNESDAY, 4 MAY 2016


1:30 – 2:15PM ORAL WITH POSTER SESSIONRoom: Willem Burger EV Isolation, Characterization and Stem Cells

Speakers: RONG XU – A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration

L. DE ROND – Label-free Detection of Extracellular Vesicles in Human Breast Milk Compared to Infant Formula

VASILIS TOXAVIDIS – Characterization of EVs by Flow Cytometry

NATASA ZAROVNI – Comparative Analysis of Urine Storage, Exosomes/EV Purification, and Normalization Methods for the Selection of Most Appropriate Solutions for Biomarker Discovery, Clinical Diagnostics and Biobanking

JENNIFER LEUNG – Quick Screening for Microparticle Content in Platelet-rich Plasma of Cardiovascular Patients

FONS VAN DE LOO – Effect of Ageing on Pro-inflammatory Micro-RNAs Contained in MSC-derived Extracellular Vesicles

MARTA ADAMIAK – Extracellular Vesicles Released by Induced Pluripotent Stem Cells Modulate Cellular Function of Recipient Cardiac Endothelial Cells

CARLA TRIPISCIANO – Pro-coagulant Activity of Platelet-derived Extracellular Vesicles

Chairs: Kenneth W. Witwer, Andrew Devitt

Room: van Weelde ORAL WITH POSTER SESSION 2 EVs, Immunity and More

Speakers: SARAH RENAUD – Induction of Tolerogenic Dendritic cells by Nasopharyngeal Carcinoma- Derived Exosomes

TOM DRIEDONKS – Immunogenic and Tolerogenic Dendritic Cells Release Extracellular Vesicles that Differ in Small Noncoding RNA Content

SIM TUNG – Exosomes Released by Regulatory T Cells Modulates T Cell Polarisation

FLORENCE TOTI – Impact of Leukocyte-derived Procoagulant Extracellular Vesicles on Endothelial Cells: Significance in Blood-mediated Pancreatic Islets Graft Reaction

JAVIER PEREZ-HERNANDEZ – Lupus Nephritis was Associated With a High Increase in the Urinary Amount of miR-146a via Exosomes Compared to Microvesicles or Cellular Pellet

INGRID GOMEZ – Neutrophil-derived-Microvesicles

Could Play a Role in the Early Stages of Atherosclerosis

NICHOLAS YOUNG – Novel Therapeutic Inhibitor Cocktail Suppresses Extracellular Vesicle-mediated Inflammation Induced by Micro-RNA in a Humanized Mouse Model of Lupus

CHERIE BLENKIRON – Characterization of Mycobacterial Membrane Vesicles

Chairs: Erzsébet Ligeti, Susanne Gabrielsson

Room: Jurriaanse ORAL WITH POSTER SESSION 3 EVs in Cancer and Other Diseases

Speakers: OCTAVIA RAMAYANTI – Circulating RNAs as Biomarkers in Epstein Barr Virus-Associated Nasopharyngeal Carcinoma

SARA ROCHA – Gastric Cancer Cell-derived Extracellular Vesicles Modulate E-cadherin Expression, Invasion and Migration of Normal Cells From the Tumor Microenvironment

GOZDE GUCLULER – D-type CpG Oligonucleotide Encapsulating and Antigen Harboring Exosomes Improve Therapy of Tumors

SOPHIE A. DUSOSWA – Repurposing Glioblastoma Exosomes as Personalized Multi-antigenic Anti-tumor Vaccine

NAO NISHIDA – Suppression of Breast Cancer Metastasis by Targeting Cancer-derived Extracellular Vesicles with Antibodies

ANNIE REBIBO-SABBAH – The Involvment of Microvesicle miRNA in Gestational Vascular Complications

DAVIDE ZOCCO – Exosome DNA: a New Promising Liquid Biopsy-based Diagnostic Tool for Personalized Management of Aggressive Prostate Cancer (PCa) Patients

Chairs: María Yáñez-Mó, Marjolein van Driel

ORAL SESSIONS2:15 – 3:00PM SYMPOSIUM SESSION 4Room: van Weelde EVs of the CNS

Speakers: EVA-MARIA KRÄMER-ALBERS – Glia-derived Exosomes Mediate Neuroprotection and Promote Axonal Transport

JOANNE WELTON – Cerebrospinal Fluid Derived Extracellular Vesicles as a Novel Source of Potential Biomarkers for Multiple Sclerosis



HONGQI XIN – Secondary Release of Exosomes From Astrocytes Contribute to Neural Plasticity and improvement of Functional Recovery After Stroke in Rats Treated with IA Administration of Exosomes Harvested From miR-133b Enriched MSCs

Chairs: Shilpa Buch, Lawrence Rajendran

Room: Jurriaanse SYMPOSIUM SESSION 5 EV Isolation

Speakers: ANIRBAN GHOSH – Novel methods of Polysaccharide- based EV Isolation: a New Opportunity for EV-based Diagnostics and Therapeutics

HYUNWOO SHIN – Prostate Cancer Diagnosis using Extracellular Vesicles Isolated by Aqueous Two-phase Systems

CARLEY ROSS – Flow Cytometric Detection and Sorting of EVs: Analysis and Characterization of Background Noise Sources That May Impact EV Fluorescence or Scatter Detection

Chairs: Jaesung Park, Andreas Möller

Room: Willem Burger SYMPOSIUM SESSION 6 EVs in Cancer Transcriptomics

Speakers: HIDETOSHI TAHARA – NGS analysis of EVs RNA in Aging and Cancer

TARAL LUNAVAT – BRAF Inhibition Regulates miR-211 Expression in Melanoma Extracellular Vesicle Subsets with Functional Consequences

CHRISTIAN PREUSSER – Selective Release of Circular RNAs in Platelet-derived Extracellular Vesicles

Chairs: Guido Jenster, Irene Bijnsdorp

3:00 – 3:30PM NETWORKING COFFEE

BIOTECH SPONSORED SESSIONS3:30 – 3:45PM BIOTECH SPONSORED SESSION 1 Room: van Weelde Caris Life Sciences

Speaker: MARK R. MIGLARESE – Adaptive Dynamic ArtificialPoly-ligand Targeting (ADAPT): A Novel Platform for Profiling Exosomes from Prostate Cancer Cells

Chairs: Juan Manuel Falcon-Perez, Gyongyi Szabo

3:30 – 4:00PM BIOTECH SPONSORED SESSION 2 Room: Jurriaanse IZON

Speaker: JULIEN MUZARD – Integrated methodology for purification, detailed biophysical characterization and phenotyping of extracellular vesicles from biological fluids

Chairs: David Lyden, Stefania Raimondo

ORAL SESSIONS3:45– 5:00PM SYMPOSIUM SESSION 7 Room: van Weelde EVs in Metabolic Syndrome, Fatty Liver Disease and Cancer

Speakers: JUSTYNA MLECZKO – Hepatocytes-derived Extracellular Vesicles From Metabolic Syndrome Models Influence Adipocytes Metabolism

SOAZIG LE LAY – Proteomic Profiling of Extracellular Vesicles Derived From Adipocytes Reveal the Presence of Key Metabolic Proteins Involved in Obesity-associated Metabolic Dysfunctions

SADHBH O'NEILL – Extracellular Vesicles, Extracellular Vesicles-contained miRNAs and Total miRNAs as Plasma-Based Biomarkers for Metabolic Syndrome

JOSHUA WELSH – Leukocyte Microvesicle as Fibrosis Biomarkers in Nonalcoholic Fatty Liver Disease

MICHAEL PLEBANEK – High-density Lipoprotein- like Nanoparticles Target SR-B1 and Inhibit Melanoma Cell-derived Exosome Uptake to Block the Pre-metastatic Niche

Chairs: Juan Manuel Falcon-Perez, Gyongyi Szabo

3:30-5:00PM SYMPOSIUM SESSION 8 Room: Willem Burger EVs and Tumor Environment I

Speakers: DOLORES DI VIZIO – Immunomodulatory Properties of Large Oncosomes From Prostate Cancer Cells

EDUARD WILLMS – Characterization and Functional Evaluation of Distinct Exosome Subpopulations

MICHAEL LIEM – Extracellular Vesicles Regulate Soluble Secreted Factors and Epigenetic Profile of the Tumor Microenvironment

YVES DECLERCK – Tumor-derived Extracellular Vesicles Captured by Mesenchymal Stromal Cells Induce an ERK1/2-dependent Pro-tumorigenic Inflammatory Reaction

MARIANA REIS-SOBREIRO – Disruption of the LINC Complex in Cancer Cells Drives the Genesis of Extracellular Vesicles with Nuclear Content

ANATOLIY SAMOYLENKO – Extracellular Vesicles as a Signaling System Involved in Kidney Morphogenesis and Disease

Chairs: Irina Nazarenko, Riccardo Alessandro

4:00-5:00PM SYMPOSIUM SESSION 9 Room: Jurriaanse EV Biomarkers for Cancer I

Speakers: FRANCESCO LOZUPONE – TM9SF4 Expression in Tumor Tissue and in Circulating Exosomes: a Novel Diagnostic Biomarker for Gastrointestinal Tumors


13


AFRODITI NANOU – Circulating Tumor Cells, Tumor Extracellular Vesicles and Serum Cytokeratins in Castration Resistant Prostate Cancer

KERSTIN MENCK – Detection and Functional Implications of Tumor-Derived Microvesicles in Ccancer Patients

EUN JU IM – Identification of Del-1 on Circulating Exosomes as a Liquid Biopsy to Detect Early Breast Cancer

Chairs: David Lyden, Stefania Raimondo

5:00 – 8:00PM POSTER SESSIONSRoom: Willem Burger Foyer/Van Der Mandele and Arcadis Zaals

Room: Willem Burger Foyer POSTER SESSION 1 Cell Biology of EVs : Biogenesis and Transfer

CORNELIA WILSON – New insights into sortilin regulation in lung cancer

MATT JOHANSEN – Biogenesis of exosomes derived from macrophages exposed to Mycobacterium avium subsp. paratuberculosis is altered in relation to infection dosage and time of exposure

APOLONIJA BEDINA-ZAVEC – Extracellular vesicles in pharmaceutical bioprocess – comparison of two cell lines

UCHINI KOSGODAGE – Inhibitors of Microvesicle Release with the Potential to Enhance Effectivity of Cancer Chemotherapy

BEATE VESTAD – Extracellular vesicles derived from SW480 cancer colon cells are internalized by human primary monocytes but not by lymphocytes

KIERAN O'DEA – Preferential uptake of circulating extracellular vesicles by lung-marginated Ly6C-high monocytes during systemic inflammation

MEA HOLM – Nogo/RTN4 as an Extracellular Vesicle-Associated Ligand

HOPE ROBERTS-DALTON – Thiol-Based Labeling of Prostate-Derived Exosomes for Analysis of Cellular Uptake and Intracellular Traffic

ALESSANDRA CIULLO – New molecular approaches to quantitatively assess exosome uptake by specific target cells

DONG SOO LEE – Cell-non-autonomous neurogenesis by convective exosomal transport of microRNA

ANNIE REBIBO-SABBAH – The involvment of microvesicle miRNA in gestational vascular complications

HONG NGUYEN – Endometrial extracellular vesicles are important for maternal-embryo communications

Chairs: Pascale Zimmermann, Charles Lai

Room: Willem Burger Foyer POSTER SESSION 2 EVs in Tumor Metastasis

SANDER STEENBEEK – Identifying RNA and protein cargo that is responsible for EV-mediated transfer of malignant properties in vivo

AINSLEY UNDERHILL – Developing high-throughput tools to evaluate exosome-induced motility in breast cancer cells

RAFAL SZATANEK – Autologous tumor-derived extracellular vesicles affect tumor cells

CATHIE GARNIS – Selective packaging of certain microRNAs into tumor microvesicles impacts tumor cell as well as adjacent stroma via TGFBR1

LINDA RIKKERT – A model to study the role of extracellular vesicles in the interaction between ovarian tumor cells and the peritoneum

MARTA RODRÍGUEZ – Exosomes enriched in stemness/metastatic-related mRNAs promote oncogenic potential in breast cancer. Specific prognostic exosome signature

AKIRA YOKOI – Malignant extracellular vesicles derived from ovarian cancer cells promote peritoneal metastasis by the breakdown of peritoneum

MAARTEN BEBELMAN – Live imaging of exosomal chemokine receptor release

TOMOHIRO UMEZU – Toward exosome-based cancer therapy: rejuvenation of exosomes derived from normal bone marrow stromal cells

YOHEI YAMAMOTO – MicroRNA-containing exosomes derived from human gallbladder cancer cell inhibit HUVEC tube formation

EVAN TAN – Jagged1 extracellular vesicles influences endothelial cell behavior by downregulating VEGFR2

FRANCESCA PERUT – Role of osteosarcoma-derived exosomes in tumor angiogenesis in vitro

SARAH RENAUD – Induction of Tolerogenic Dendritic cells by Nasopharyngeal Carcinoma-Derived Exosomes

ANASTASIA MALEK – Breast cancer cell migration is induced by exosomes via surface interaction and through the activation of FAK signalling

VERA REBMANN – Clinical and functional aspects of HLA-G positive extracellular vesicles in breast cancer

Chairs: Jameel Inal, Mercedes Tkach

Room: Willem Burger Foyer POSTER SESSION 3 Analysis of EVs in Body Fluids

ZUZANA KRUPOVA – An improved method to isolate milk-derived extracellular vesicles to study the impact of a null allele at the CSN1S1 locus on their secretion, size distribution and molecular content



KARI BENTE FOSS HAUG – CD9-positive EVs isolated from size exclusion chromatography (SEC)-precleared plasma from patients and healthy individuals reveal microRNA (miR) patterns

BIRGIT FENDL – Influence of anticoagulation on microvesicle release in whole blood

TATIANA ALTADILL – Enabling Metabolomics Based Biomarker Discovery Studies using Molecular Phenotyping of Exosome like vesicles

REMI RICHARD – Advances in EV isolation from human plasma using the Vn96 synthetic peptide

ELISA GIACOMINI – Getting insight of uterine fluid-derived extracellular vesicles

MUSANTE LUCA – Characterization of Residual Urinary Extracellular Vesicles in Ultracentrifugation Supernatants

LEI ZHENG – Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum

FELIX ROYO – Comparison and miRNA profiling of extracellular vesicles isolated from human urine with five different methods compatible with clinical settings

ERIOMINA SHAHAJ – Detection of MicroRNA in plasma extracellular vesicles (EV)

ROCCO LUCERO – ExRNA Virtual Biorepository

SANG KYUNG KIM – A highly multiplexed real-time PCR demonstrated with quantitative profiling of miRNAs and DNAs from extracelluar vesicles.

DOROTA TATARUCH-WEINERT – Urinary extracellular vesicles: RNA isolation shows gender related yield profiles

DILLON MUTH – Influence of Menstrual Cycle and Retrovriral Infection on Cervicovaginal Extracellular Vesicles in Primates

Chairs: Martijn van Herwijnen, Sergei Kruglik

Room: Willem Burger Foyer POSTER SESSION 4 EVs as Drug Delivery System for Antitumoral Therapies & Vaccination

SU CHUL JANG – siRNA Delivery by Exosome-Mimetic Nanovesicles – Implications for Targeting c-Myc in Cancer

GIULIA CORSO – Comparing the loading efficiency of exosomal protein chimeras

TAKUMA YAMASHITA – Effect of exosome isolation methods on their yield, physicochemical and pharmacokinetic properties

MAX PIFFOUX – Extracellular vesicles as biological theranostic nano-vectors for tumor therapy: comparison of production and purification methods

SANDER KOOIJMANS – Recombinant phosphatidylserine-binding proteins for targeting of extracellular vesicles to tumor cells: A plug-and-play approach

NAO NISHIDA – Suppression of breast cancer metastasis by targeting cancer-derived extracellular vesicles with antibodies

CHRISTINA SCHINDLER – Exosomal Doxorubicin demonstrates improved tumour cell potency, but reduced cardiotoxicity compared to other Doxorubicin formulations

SOPHIE A. DUSOSWA – Repurposing glioblastoma exosomes as personalized multi-antigenic anti-tumor vaccine

STEFANIE HILTBRUNNER – Exosome based cancer immunotherapy is independent of exosomal MHCI

STEFANIA RAIMONDO – Inhibition of in vitro and in vivo Chronic Myeloid Leukemia cell growth by IL3R-targeted exosomes

MASAKI MORISHITA – Induction of potent antitumor immune response by development of CpG DNA-conjugated tumor cell-derived exosomes

KATHRIN GÄRTNER – Recombinant exosomes carrying CD40L and herpesviral proteins redirect antiviral immune responses towards malignant B-CLL cells: An immunotherapeutic approach

MATTHIAS GERRITZEN – Meningococcal outer membrane vesicles as vaccine platform candidate

FABIO ANTENUCCI – Role of degS and nlpI in outer membrane vesicle production and cell-to-cell interactions in Actinobacillus pleuropneumoniae

NICHOLAS YOUNG – Novel therapeutic inhibitor cocktail suppresses extracellular vesicle-mediated inflammation induced by micro-RNA in a humanized mouse model of lupus

Chairs: David Carter, Veronica Hubert

Room: Willem Burger Foyer POSTER SESSION 5 EVs in Acute and Chronic Inflammation

KATALIN SZABÓ-TAYLOR – Redox state of exofacial molecules on leukocyte-derived EVs in inflammation

ROBERTA TASSO – Mesenchymal stem cell-derived microvesicles exposed to normoxic and hypoxic culture conditions are involved in macrophage polarization

CSABA I TIMAR – Distinguishing PMN derived activated EVs from apoptotic vesicles

KHALED ALGHAREEB – Investigating the Apoptotic cell-derived extracellular vesicles with ICAM3 on apoptotic cell clearance

GANESH SHELKE – DNA on the extracellular vesicle surface

SILVIA CONSALVI – Exosomes mediate the cross-talk between cell populations in muscle that contribute to Duchenne Muscular Dystrophy progression

ABHAY KUMAR – Immuno-modulatory role of exosomes in Tuberculosis



E. MARION SCHNEIDER – Microparticles derived from cultured macrophages and cerebrospinal fluid of patients with schizophrenic and affective disorders

PIERRE HARDY – The miRNAs are selectively enriched in retinal pigment epithelium cell-derived microparticles and mediate oxidative stress-induced retinal cells dysfunction

LACHLAN PEARSON – Identification and Proteomic Profiling of Extracellular Vesicles in Peritoneal Dialysis Fluid

LUCIA TERLECKI-ZANIEWICZ – The extravesicular miRNome of senescent human fibroblasts and its impact on keratinocyte functionality

JAVIER PEREZ-HERNANDEZ – Lupus nephritis was associated with a high increase in the urinary amount of miR-146a via exosomes compared to microvesicles or cellular pellet

ANTJE R. WESELER – Exposure of lung epithelial cells to man-made mineral fibers elicits the release of extracellular vesicles with pro-inflammatory properties

GYONGYI SZABO – MicroRNA cargo of extracellular vesicles from alcohol-exposed monocytes signals naïve monocytes to differentiate into M2 macrophages

REBECCA M. WADEY – Hypoxia and TNFa co-treatment affects the number and size of adipocyte-derived EV

MALENE MOELLER JØRGENSEN – Can blood-brain barrier disruption and active inflammation in multiple sclerosis be monitored by EVs in plasma?

MUTHUVEL JAYACHANDRAN – Inflammation- and thrombin- induced endothelium-derived microvesicles and modulation of endothelial barrier function in human brain microvasculature

Chairs: Vicky Yang, Paola de Candia

Room: Van Der Mandele POSTER SESSION 6 and Arcadis Zaals EV Proteomics & Lipidomics

CONNIE JIMENEZ – Peptide-mediated ‘miniprep’ isolation of extracellular vesicles for high-throughput proteomics; method evaluation and application in colon cancer

ZHIGANG SUI – Simple and effective isolation of exosomes by polyethylene glycol from cell culture supernatant for in-depth proteome profiling

JOANNA KASPRZYK – Simplified method for proteome analysis of platelet induced membrane vesicles

JOSHUA BRZOZOWSKI – Lipid profiling of normal and tumourigenic cell-derived extracellular vesicles reveals differences in composition

ALEKSANDER CVJETKOVIC – Proteomics of subpopulations of extracellular vesicles: Clues of subcellular origin

ALEMAYEHU GODANA BIRHANU – Proteomic Analysis of Mycobacterium tuberculosis Membrane Vesicles

HERNANDO A. DEL PORTILLO – Proteomic analysis of human reticulocyte-derived exosomes

YI XIN FIONA LEE – Proteomics profiling of mouse pluripotent stem cells and their extracellular vesicles

ADRIANA PAES LEME - Cell confluence affects the proteome of extracellular vesicles from oral cancer cells

TAKASHI SHIROMIZU – Targeted proteomic analysis for biomarker discovery of colorectal cancer metastasis from plasma EVs

SHONA PEDERSEN – Proteomic characterization of Circulating Extracellular Vesicles in Multiple Myeloma

ROBERTA FREEZOR – Erythrocyte cells and HRV16 infected HeLa cells derived microvesicles protease profiling

LAKSHMI KRISHNAMOORTHY – Proteomic analysis of redox signaling in extracellular vesicles

ERJA KERKELÄ – Phospholipid profiles of red blood cells and their extracellular vesicles are not significantly changed during storage of red cell concentrates

JULIA COSTA – Extracellular vesicles from tumor cells display specific glycosignatures

Chairs: Kwang Po Kim, Amal Al-Dossary

Room: Van Der Mandele POSTER SESSION 7 and Arcadis Zaals EV Isolation

ELENA KHOMYAKOVA – Minimizing the loss of exosomes during their storage at 4°C.

RONG XU – A protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration

VILDAN CANER – An ultracentrifugation-based protocol for exosome isolation in diffuse large B-cell lymphoma

ELIZABETH GRAY – Methodological comparison of CSF extracellular vesicle extraction as a novel source of biomarkers in amyotrophic lateral sclerosis

MAIJA PUHKA – Comparison of a fast column-based EV isolation method with ultracentrifugation and size-exclusion

RUZENA STRANSKA – Comparison of exoEasy Maxi kit (Qiagen) with size-exclusion chromatography qEV columns (Izon Science) for isolation of extracellular vesicles from human plasma

VERENA BÖRGER – Comparison of isolation methods for EVs from fresh and frozen serum and plasma samples

ANA GÁMEZ-VALERO – Impact of SEC, PEG and PROSPR isolation methods on Extracellular Vesicles: A Comparative Study

NATASA ZAROVNI – Comparative analysis of urine storage, exosomes/EV purification, and normalization methods for the selection of most appropriate solutions for biomarker discovery, clinical diagnostics and biobanking



SEBASTIAN BOROSCH – Isolating Exosomes from cell culture supernatant: A comparison of commonly used protocols

ROMAIN LINARES – Isolation of extracellular vesicles from plasma using magnetic nanoparticles functionalized with Annexin-A5

HEIKKI SAARI – Improved large-scale EV production using a two-compartment bioreactor

NICHOLAS ANGELONI – Rapid detection and enrichment of tumor-derived exosomes using targeted high-density lipoprotein-like gold nanoparticles

FEDERICA DE LISE – Isolation and purification of extracellular nanostructures from N.sp.PP1Y: a novel example of Outer Membrane Vesicles?

JOHN CADWELL – Large scale production of extracellular vesicles in a hollow fiber bioreactor

CHIHCHEN CHEN – Development of an Automated Microfluidic Platform for the Isolation of Extracellular Vesicles

Chairs: Cecilia Lässer, Anirban Banerjee

Room: Van Der Mandele POSTER SESSION 8 and Arcadis Zaals EVs in Prostate Cancer

MIREIA OLIVAN – Identification of novel biomarkers by comprehensive proteomics in urinary exosomes for prostate cancer detection

IRENE BIJNSDORP – Identification of prostate cancer protein biomarkers by proteomics of urinary extracellular vesicles

ROSETTA MARTINIELLO-WILKS – The potential of urinary exosomal miRNAs as biomarkers for prostate cancer

REMI RICHARD – A novel urinary EV-based prostate cancer assay that correctly predicts cancer grade as identified in the biopsy sample

ALICIA LLORENTE – Exosomal proteins as prostate cancer biomarkers in urine: From mass spectrometry discovery to antibody-based validation

CAROLINA SOEKMADJI - Identifying extracellular vesicles-derived miRNAs in advanced prostate cancer

PEDRO FONSECA – Prostate Cancer Exosomes As Molecular Predictors Of Response To Abiraterone Acetate

VINCENT YEUNG – Identifying components that regulate the secretion of stroma-activating exosomes in prostate cancer

JOSEPHINE SALIMU – Immunosuppressive effects of tumour exosomes on dendritic cells cross-presenting tumour antigens

DAVIDE ZOCCO - Exosome DNA: a new promising liquid biopsy-based diagnostic tool for personalized management of aggressive Prostate Cancer (PCa) patients

DESMOND PINK – Development of a biomarker-based extracellular vesicle assay for prostate cancer prognosis

ALEXANDRA SHEPHARD – Can exosome-associated heparan sulphate proteoglycans serve as potential new markers for the detection of prostate cancer?

IHSAN EL SAYED – CRIPTO 1 is Associated with Tumor Aggressiveness in Prostate Cancer

IRENE TATISCHEFF – Innovative approach of prostate cancer by the way of tumor cell-derived extracellular vesicles (EVs)

Chairs: Rubina Baglio, Martin Van Royen

6:30-8:30PM SATELLITE EVENT Room: Jurriaanse Meet the National and International Societies

This event will provide an opportunity for scientific networks and consortia focusing on extracellular vesicles to introduce themselves to the ISEV community.

Chair: Lorraine O’Driscoll, María Yáñez-Mó

AUSTRALASIA EXTRACELLULAR VESICLES Representative: Prof. Andy Hill

GEIVEX Representative: Prof. Hernando A. del Portillo

H2020 COST ACTION MICROVESICLE & EXOSOMES IN HEALTH AND DISEASE (ME-HAD) Representative: Prof. Lorraine O'Driscoll

JAPANESE SOCIETY FOR EXTRACELLULAR VESICLES (JSEV) Representative: Prof. Takahiro Ochiya

KOREAN SOCIETY FOR EXTRACELLULAR VESICLES (KSEV) Representative: Prof. Yong Song Gho

REGIONAL RESEARCH NETWORK ON EXTRACELLULAR VESICLES (RRNEV) Representative: Prof. Reidun Ovstebo

SOCRATES Representative: Prof. Tan Kok Hian

UKEV FORUM Representative: Dr. Dave Carter

A FRENCH EV SOCIETY Representatives: Dr Guillaume van Niel and Clotilde Théry




8:00 – 8:45AM MEET THE EXPERTS During the 2015 annual meeting, ISEV launched the new "Meet the Expert" sessions. These sessions were extremely successful and well received. This year ISEV decided to further develop these interactive conversations with the delegates on the state-of-the-art and future goals of extracellular vesicle research.

Room: Willem Burger MEET THE EXPERTS SESSION 1 EV Heterogeneity: Can we Overcome the Confusion?

Speakers: Clotilde Théry, Dolores Di Vizio

Moderator: Andrew Hill

Room Jurriaanse MEET THE EXPERTS SESSION 2 EVs in Regenerative Medicine

Speakers: Giovanni Camussi, Sai Kiang Lim

Moderator: Eva Rohde

Room: van Weelde MEET THE EXPERTS SESSION 3 EVs in Infections Disease

Speakers: Ana Claudia Torrecilhas, Yong Song Gho

Moderator: Hernando Del Portillo

9:00 – 10:15AM ORAL SESSIONSRoom: Jurriaanse SYMPOSIUM SESSION 10 EVs in Cross-species Communication

Speakers: VANESSA DINIZ ATAYDE – Non-enveloped Leishmania RNA Virus 1 (LRV1) is released by Leishmania parasites within exosome-like vesicles

FONS A.J. VAN DE LOO – Extracellular vesicles from milk accelerate osteoblastogenesis but impair bone matrix formation

CHERIE BLENKIRON – Assessment of the transcriptional response of human bladder cells to uropathogenic Escherichia coli membrane vesicles

FRANKLIN WANG-NGAI CHOW – Investigation of extracellular small RNAs of Heligmosomoides polygyrus: Scope for RNAi mediated cross-species communication

REGIS STENTZ – Gut bacteria generated extracellular vesicles mediate cross-kingdom dialog in the gut

Chairs: Yong Song Gho, Ana Claudia Torrecilhas

Room: Willem Burger SYMPOSIUM SESSION 11 EV Characterization: Flow Cytometry

Speakers: ANDRÉ GÖRGENS – Optimization of Imaging Flow Cytometry for Exosome Analysis using a Biological Calibrator

AIZEA MORALES-KASTRESANA – Optimization of bulk extracellular vesicle labels for nanoFACS

JOHN NOLAN - Diversity of Extracellular Vesicles in Biofluids Measured by Vesicle Flow Cytometry

STEN LIBREGTS – Towards EV-based biomarker detection using high-resolution flow cytometric analysis of rare events

ELMAR GOOL – Surface plasmon resonance imaging detects lower antigen densities than flow cytometry

Chairs: Marca Wauben, Alain Brisson

Room: van Weelde SYMPOSIUM SESSION 12 EVs in Cardiovascular Disease

Speakers: INGRID BANK – An extracellular vesicle protein signature as a new diagnostic biomarker for stable coronary artery disease

MUTHUVEL JAYACHANDRAN – Coronary artery calcification associates with specific cell-derived blood-borne microvesicles in middle aged women with and without history of preeclampsia

PETER QUESENBERRY – Exosomes induce and reverse monocrotaline-induced pulmonary hypertension in mice

MARIA FELICE BRIZZI – Trafficking of STAT5 via endothelial cell-derived extracellular vesicles regulates IL-3 paracrine action

ANNA SHARROCK – Human endothelial microvesicle shedding in response to simulated blast trauma

Chairs: Ramaroson Andriantsitohaina, Susmita Sahoo


10:45 – 12:30PM PLENARY SESSION 2 Room: Willem Burger Learning from the Best: Viruses and EVs

Speakers: ROBERT C. GALLO M.D. is professor and director of the Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland and Scientific Director of the Global Virus Network - Pathogenic Retroviruses: What they can do and What we can do about them


THURSDAY, 5 MAY 2016


LEONID MARGOLIS PH.D. DSC. is an Adjunct Scientist of MSU and the Ilia University of the Republic of Georgia. - Extracellular vesicles and viruses: cousins in complicated relations

Chairs and Introduction to the speakers: Esther Nolte-‘t Hoen and Jennifer Jones

12:30 – 1:30PM LUNCH

1:30 – 3:00PM ORAL SESSIONS

Room: van Weelde SYMPOSIUM SESSION 13 EVs in Remodeling and Repair

Speakers: FELIX ROYO – Hepatocyte derived extracellular vesicles enzymatic activity modify serum metabolome

STEVEN JAY – Controlled Enhancement of EV Vascularization Bioactivity by Ethanol Conditioning

THOMAS RITTER – Human mesenchymal stem cell-derived extracellular vesicles ameliorate eye disease by reducing inflammation and neovascularisation in a corneal alkali burn rat model after topical administration

ELGA BANDEIRA – Treatment with extracellular vesicles from adipose tissue derived mesenchymal stromal cells in a murine model of silicosis

ENRICO RAGNI – Mesenchymal stromal cell-derived extracellular vesicles as vehicle of therapeutic mRNA: evidences for Walker-Warburg muscular dystrophy treatment

REBECCA LIM – Characterisation of exosomes from human amnion epithelial cells

Chairs: Peter Quesenberry, Fabiana Geraci

Room: Jurriaanse SYMPOSIUM SESSION 14 Engineering EVs

Speakers: OH YOUN KIM – Genetically engineered bacterial protoplast-derived nanovesicles that mimic extracellular vesicles as targeted drug delivery system

CHRISTINA SCHINDLER – High affinity single-chain fragment variable (scFv) are novel specific targeting motifs for extracellular vesicles

IKUHIKO NAKASE – Effective cellular uptake of biofunctional peptide-modified exosomes

CHULHEE CHOI – Efficient and rapid cellular delivery of bioactive proteins using EXPLOR: exosomes engineered for protein loading via optically reversible protein–protein interaction

MARIE DIDIOT – Exosome-mediated delivery of hydrophobically modified siRNA for Huntingtin mRNA silencing

AMANDA SILVA – Customizing extracellular vesicles for therapy and imaging

Chairs: Hubert Yin, Raymond Schiffelers

Room: Willem Burger SYMPOSIUM SESSION 15 EVs and Tumor Environment II

Speakers: OLGA VOLPERT – Exosomes from non-metastatic melanoma recruit patrolling monocytes for immune clearance of metastasis

VERONICA HUBER – Exploitation of exosomes as delivery system of functional pro-apoptotic membrane TRAIL

OLIVIER MORALES – In vitro and In vivo Evaluation of EBV latency II-derived peptides as Tumor Vaccine for Immunotherapy of Nasopharyngeal Carcinoma

HINRICH HANSEN – The shuttle of extracellular vesicles between cancer and bystander cells allows dual targeting of malignant and supporter cells of the tumor microenvironment by antibody drug conjugates

VALENTINA R MINCIACCHI – Large oncosomes reprogram prostate fibroblasts toward an angiogenic phenotype

IRINA NAZARENKO – Molecular Mechanisms of the Mesenchymal-Epithelial Transition and Exosome-Driven Signaling in Breast Cancer Metastases

Chairs: Lorraine O’Driscoll, Hector Peinado


3:30 – 4:30PM SYMPOSIUM SESSION 16Room: van Weelde New Techniques for EV Characterization

Speakers: MARTA BALLY – A nanofluidic device to study of extracellular vesicles on a single vesicle basis

EDWIN VAN DER POL – Size and refractive index determination of extracellular vesicles smaller than 70 nm in a nano-fluidic optical fiber

BAHARAK HOSSEINKHANI – A Biosensor study of Extracellular Vesicles derived from inflammation-triggered endothelial cells.

THOMAS HARTJES – EVQuant: A novel extracellular vesicle quantification and characterization assay for scientific and diagnostic use

Chairs: Martijn van Herwijnen, Frank Coumans



Room: Jurriaanse SYMPOSIUM SESSION 17 EV Biomarkers for Cancer II

Speakers: THØGER NIELSEN – EV-mediated procoagulant activity in multiple myeloma and its precursor monoclonal gammopathy of undetermined significance (MGUS)

ANNE FLOU KRISTENSEN – Extracellular Vesicles mediate a hypercoagulable profile in Small Cell Lung Carcinoma patients

CLARK CHEN – Cerebrospinal fluid microRNA (CSF miRNA) signature as a biomarker for glioblastoma detection

STEPHAN STREMERSCH – Identification of individual exosome like vesicles by surface enhanced Raman spectroscopy

Chairs: Hidetoshi Tahara, Leonora Balaj

Room: Willem Burger SYMPOSIUM SESSION 18 EVs in Neuropathies

Speakers: SARAH FERNANDO – Exosomes and microvesicles from ALS CNS tissues contain misfolded SOD1 and transmit intracellular propagated misfolding

LYDIA ALVAREZ-ERVITI – Influence of Parkinson's pathology upon exosomal alpha-synuclein

ALEXANDER HARTMANN – PrPC on exosomes drives fibrillization of amyloid beta and ameliorates amyloid beta-mediated neurotoxicity

MICHAEL GRANER – Glioblastoma extracellular vesicles drive normal astrocytes toward a tumor-enhancing phenotype

Chairs: Andrew Hill, Eva-Maria Krämer-Albers


Room: Van Der Mandele POSTER SESSION 1 and Arcadis Zaals EVs, Microbes & Cross-species Communication

ELINE PALM HANSEN – Identification of extracellular vesicles in three parasitic nematodes of veterinary importance

VICTORIA GILLAN – Examining the exosome cargo of Theileria annulata infected cells

RAYA SORKIN – Mechanics of extracellular vesicles derived from malaria parasite-infected Red Blood Cells

ANDRÉ CRONEMBERGER ANDRADE – The immunomodulatory role of human macrophages exomes during Trypanosoma cruzi infection

ROSANA PUCCIA – Characterization of extracellular vesicles from stressed pathogenic fungal cells

BEN PEACOCK – Characterisation of extracellular vesicles secreted by HPV+ and HPV- oropharyngeal carcinoma cells

CORINNA HÜLS – Functional analysis of packaged viral RNAs from Epstein-Barr-Virus (EBV) by using recombinant exosomes

MEGHAN TRAVERS – Cellular Survival and Response to HIV Infection in the Absence of Extracellular Vesicles

RAEDEEN RUSSELL – The Role of Exosomes in the Development of Chlamydia-induced Upper Genital Tract Pathology

HEA YOON KWON – Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi

JE CHUL LEE – Cytotoxicity of host cells induced by Staphylococcus aureus membrane vesicles is dependent on proteomes of membrane vesicles

FORUGH VAZIRISANI – Extracellular vesicles from gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis induce a strong inflammatory response and cell death in vitro

CHARLOTTE VOLGERS – Membrane vesicles released during macrophage-infection with airway pathogens modulate innate immune responses

DENIS SIMONOV – Bacterial membrane vesicle RNA in host-pathogen interactions: on the road towards specific functional studies

FONS VAN DE LOO – Extracellular vesicles from milk promote the formation of small osteoclasts but reduce bone resorption

KATE TIMMS – Cross-kingdom uptake and transfer of exosomal watermelon microRNAs in human intestinal epithelial cells

Chairs: Leonid Margolis, Oh Youn Kim

Room: Van Der Mandele POSTER SESSION 2 and Arcadis Zaals EVs in Cardiovascular Disease

RAMAROSON ANDRIANTSITOHAINA – Obligatory role of mitochondrial cross-talk with endoplasmic reticulum in the regulation of oxidative stress leading to endothelial dysfunction by human microparticles

SIHEM SADOUDI – An Imbalance Between Circulating Microparticles and Annexin-A5 May Promote Vascular Injury During Sickle Cell Disease

GAIA PAPINI – Exosomes released from sulforaphane-treated fibroblasts protect the cardiomyocytes from angiotensin II-induced hypertrophy

SANDRA KRAEMER – Preconditioning with isoflurane and hypoxia: a possible role of exosomes?



LIN CUI – Matrix Vesicle-like Particles Are Associated With Aortic Valve Calcification

GOSIA FURMANIK – Endoplasmic Reticulum Stress in Vascular Smooth Muscle Cells Regulates Exosome Release and Results in Calcification

LAURA DOWNIE – Extracellular vesicles isolated from conditioned media from cells over-expressing osteopontin promote an increase in cardiomyoblast cell size

BEN WARD – Neutrophil microvesicles preferentially adhere to endothelium exposed to disturbed flow

DANIELSON KIRSTY – A functional role for extracellular vesicles in heart failure and cellular electrical remodeling

VICKY YANG – Exosomal miRNA as liquid biomarker for canine arrhythmogenic right ventricular cardiomyopathy (ARVC) in Boxer Dogs

IDA-LIISA KOLARI – Processed small hairpin RNAs are secreted from transduced cells to surrounding tissue in vivo in mouse myocardial infarction model

VICKY YANG – Valve interstitial cell (VIC) miRNA profile and phenotype are affected by canine Wharton’s Jelly mesenchymal stem cell conditioned media (WJ-MSC CM) in canine myxomatous mitral valve disease (MMVD)

RAMAROSON ANDRIANTSITOHAINA –Tumour necrosis factor-a carried by microparticles from apoptotic RAW 264.7 macrophage cells triggers deleterious effects on cardiomyocytes from adult mice

PETER QUESENBERRY – Bone marrow endothelial progenitor cells are the cellular mediators of pulmonary hypertension in the murine monocrotaline injury model

AINA MARTIN – Non-canonical WNT signaling is mediated by extracellular vesicles in pulmonary fibrosis

Chairs: Pia Siljander, Bas van Balkom

Room: Van Der Mandele POSTER SESSION 3 and Arcadis Zaals EVs in Cardiovascular Disease and Coagulation

INGRID GOMEZ – Neutrophil-derived-microvesicles could play a role in the early stages of atherosclerosis

XAVIER LOYER – Endogenous cardiac extracellular vesicles are transiently released during experimental myocardial infarction in mice

ADELINE WANNEZ – Role of extracellular vesicles in thrombosis in paroxysmal nocturnal hemoglobinuria patients

RIENK NIEUWLAN – Platelet-derived extracellular vesicles exposing fibrinogen may be a potential marker for ongoing thrombus formation

SALEH NASIRI – Flow cytometric measurement of CD41/CD61 and CD42b major platelet receptors of infusible platelet membrane as a platelet substitute

ANNALISA RADEGHIERI – Exploring the phenotypic assessment of Antithrombin deficiency by means of exosomes

AIGLI EVRYVIADOU – A novel thrombo-inflammatory pathway for monocyte recruitment mediated by platelet-derived vesicles

RENÉ WEISS – Citrate concentration influences platelet activation and microvesicle generation during lipid apheresis

CARINA LEVIN – Extracellular vesicles characterization and possible pathogenic role in ß-Thalassemia Major

CARLA TRIPISCIANO – Pro-coagulant activity of platelet-derived extracellular vesicles

FLORENCE TOTI – Impact of Leukocyte-derived procoagulant extracellular vesicles on endothelial cells: significance in blood-mediated pancreatic islets graft reaction

NIKOL SULLO – Microvescicles and Microvescicles associated miRNA as biomarker of acute kidney injury in a paediatric population following cardiac surgery.

MITJA DRAB – Decrease of concentration of extracellular vesicles in blood isolates of 27 athletes one day after long distance running

BAREND W. FLORIJN – Altered distribution of HDL- and extracellular vesicle associated circulating microRNAs in diabetic nephropathy and systemic microvascular damage

JENNIFER LEUNG – Quick screening for microparticle content in platelet-rich plasma of cardiovascular patients

JACO BOTHA – Levels of circulating microvesicles are associated with plasma lipids and ectopic fat accumulation in type 2 diabetic men with low testosterone and are unchanged after testosterone replacement therapy

Chairs: Rienk Nieuwland, Saara Laitinen

Room: Willem Burger Foyer POSTER SESSION 4 EVs in Stem Cells

DANIELA CESSELLI – Extracellular vesicles produced by the microenvironment of low-grade gliomas at different risk of anaplastic progression differ in terms of miRNA content

DANIELA CESSELLI – Extracellular vesicles released by Glioma Stem Cells activate astrocytes and induce the acquisition of neural stem-like potential by astrocytes.

DANIELA CESSELLI – Extracellular vesicles released by stem cells isolated from the glioma microenvironment enhance the in vivo aggressiveness of glioblastoma cells.

SARAH HEILER – The contribution of the cancer initiating cell markers EpCAM and claudin7 to metastatic progression



ÁGOTA TUZESI – Paediatric brain cancer stem cells and their exosomal miRNA content

CAROLINA BALBI – The regenerative potential of the amniotic fluid stem cell microvesicles

SOAZIG LE LAY – Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway

JAE MIN CHA – Augmented production of mesenchymal stem cell-derived therapeutic extracellular vesicles via 3D-bioprocess

MARTIN-DUQUE PILAR – Exosomes from placental MSCs as vehicles for the Na/I symporter (hNIS): A new antitumoral therapy.

FONS VAN DE LOO – Effect of ageing on pro-inflammatory micro-RNAs contained in MSC-derived extracellular vesicles

ULRIKE ERB – Cancer stem cell markers and exosomes

VASILIKI MACHAIRAKI – Human iPSC-derived extracellular vesicles: unique properties and biological activities in vitro and in vivo

MARTA TAPPARO – Extracellular vesicles released by renal cancer stem cells inhibit immune response

CHIARA GAI – Role of argonaute 2-Alix interaction in miRNA enrichment in extracellular vesicle released by human liver stem cells

GARETH WILLIS – Regulation of macrophage polarization by mesenchymal stem cell derived-exosomes

Chairs: Ewa Stepien, Steven Jay

Room: Willem Burger Foyer POSTER SESSION 5 EVs as Cancer Biomarkers

RIKKE BAEK – Extensive phenotyping of plasma EVs from Small Cell Lung Cancer patients undergoing treatment

MALENE MOELLER JØRGENSEN – Potentials of plasma EVs in relation to diagnostics and prognostics in lung cancers

FRANCO GRIMOLIZZI – miR126 is mainly delivered through exosomes in early-stage NSCLC patients

JAE YOUNG HUR – Tissue-compatible EGFR genotyping in tumor-derived extracellular vesicles from malignant pleural effusions

OCTAVIA RAMAYANTI – Circulating RNAs as biomarkers in Epstein Barr virus-associated nasopharyngeal carcinoma

IOULIA VARDAKI – Molecular profiling of metastatic versus non-metastatic breast cancer cell derived exosomes

NATASA ZAROVNI – Development of blood exosomes based immunoassays and PCR tests for active surveillance of neurofiboromatosis patients and early detection of malignant peripheral nerve sheet tumors (MPNST)

ANGELA DI GIANNATALE – Characterization of microRNAs and proteomic cargo in exosomes secreted by neuroblastoma

YUSUKE YOSHIOKA – Circulating cancer-associated extracellular vesicles as early detection biomarkers for pancreatic ductal adenocarcinoma

FEDERICA RICCARDO – Plasma Derived Exosomes as a Reservoir of Biomarkers for Leukemia Patients Stratification

CONNIE JIMENEZ – Exosomes secreted by apoptosis-resistant AML blasts harbor regulatory network proteins potentially involved in antagonism of apoptosis

MONIQUE AJ VAN EIJNDHOVEN – Plasma EVs as a liquid biopsy source for RNAseq-based detection of cancer-associated microRNAs

CHRISTINE COTICCHIA – Depletion of normal blood cell-derived exosomes from plasma to enhance a tumor-associated exosomal mRNA signature

TONJE BJØRNETRØ – Exosomes from hypoxic colorectal cancer (CRC) cells – miRNA cargo as indicator of adverse CRC biology?

RANDY CARNEY – Targeting tumor-associated exosomes with novel integrin binding peptides

JUAN HIDALGO DE QUINTANA – High-throughput, Purification-free, Multiplexed Profiling of Circulating miRNA for Discovery, Validation, and Diagnostics

MAIJA PUHKA – Metabolomics of urinary EVs: main pathways, normalization and potential for biomarker studies

Chairs: Casey Maquire, Carolina Soekmadji

Room: Willem Burger Foyer POSTER SESSION 6 EVs in the CNS

RITA PERFEITO – Autophagy activation prevents alpha-synuclein secretion in exosomes

AARON BRADSHAW – Investigation of the role exosomes play in the propagation of tau pathology

DAISY SPROVIERO – Microvesicles in amyotrophic lateral sclerosis: potential biomarkers for disease propagation and therapeutic targets

ANNA GHARIBYAN – Exosomes as a source of new biomarkers and candidate therapeutic targets in Parkinson’s disease

MANDY PEREIRA – Salivary vesicles expression in traumatic brain injury

JULIE SAUGSTAD – MicroRNAs in human cerebrospinal fluid as biomarkers for Alzheimers disease

MICHAEL BUCKLAND – Serum exosomal RNA as a biomarker in multiple sclerosis

SANDRA DEN HOEDT – ApoE-containing particles excreted by astrocytes and neurons: Exosomes or lipoproteins?

TANINA ARAB – Proteomics characterization and functional assays of leech microglia extracellular vesicles



MUTHUVEL JAYACHANDRAN – Microvesicles derived from specific cells of the neurovascular unit associate with white matter hyperintensities in brains of middle aged women with and without history of preeclampsia

GIORGIO BERGAMINI – Extracellular vesicles as mediators of periphery to brain communication: relevance for stress-induced behavioural disorders

Chairs: Roosmarijn Vandenbroucke, Julie Saugstad

Room: Willem Burger Foyer POSTER SESSION 7 EV Characterization From Different Sources or Different Subtypes

EDUARDA GUERREIRO – Extracellular vesicles from oral squamous cell carcinoma cells in culture: isolation and characterization

JONATHAN DEGOSSERIE – Role of extracellular vesicles in epithelial-endothelial communication during thyroid development

JUNJI HAMURO – Concomitant Evaluation of a panel of exosome proteins and miRs for qualification of cultured human corneal endothelial cells

SYLWIA KONIUSZ – Visualization and characterization of extracellular vesicles derived from human bone marrow mesenchymal stem cells using different staining analysis

KENTARO JINGUSHI – Analysis of extracellular vesicles obtained from clear cell renal cell carcinoma tissue

JASON MIGHTY – Characterization of Retinal Progenitor Cell Microvesicle Content

CHRISTINE TUCHER – Differential characterization of microvesicles and exosomes from activated and apoptotic human lymphocytes

CHERIE BLENKIRON – Characterization of Mycobacterial Membrane Vesicles

STEFAN WILD – Diversity of extracellular vesicles addressed by immunoaffinity beads

MORAYMA TEMOCHE-DIAZ – Fractionation of discrete extracellular vesicle sub-populations reveals distinct protein and RNA profiles

RONNE YEO – MSC secretes at least three EV types each with a unique permutation of membrane lipid, protein and RNA

ELISA LAZARO-IBAÑEZ - Presence of DNA in subsets of exosomes-like extracellular vesicles

ANNA SAVELYEVA – Transcriptome of different types of human blood circulating complexes

FABIO ALEXIS LEFEBVRE – Comparative transcriptomic analysis of human and Drosophila extracellular vesicles reveals extensive conservation

SU CHUL JANG – Membrane proteomics of extracellular vesicles

Chairs: Masood Kamali-Moghaddam, Takahori Ichiki

Room: Willem Burger Foyer POSTER SESSION 8 Late Breaking Poster Session I

ELENA KHOMYAKOVA – Surface markers profiling of prostate cancer exosomes

XABIER OSTEIKOETXEA - Detection and characterization of extracellular vesicles in pancreatic juice and blood of patients with pancreatic cancer

YE-RIN KO – Breast cancer cell-derived exosome secretion is regulated by intracellular calcium concentration

MYRON BEST – Tumor-education of platelets by pre-mRNA splicing and alternative RNA processing enables blood-based detection of cancer

HIROKO TADOKORO – Metabolomic profiling of breast cancer-derived extracellular vesicles

PAOLO GUAZZI – EXO-DNA applications: a novel EV-based method for detection of actionable mutations from the blood of cancer patients

THEOCHARIS PANARETAKIS – Energy-requiring uptake of prostasomes and PC3 cell-derived exosomes into non-malignant and malignant cells

RAHUL BHOME – Exosomes and microparticles: extracellular vesicles with functionally different roles in colorectal cancer progression.

YOSHIYUKI KOYAMA – "Artificial neoepitope"- bearing exosomes derived from tumor cells being genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy

CUTRONE ROCHELLE – Extracellular vesicles are involved in MAPC® promotion of oligodendrocyte differentiation in vitro

ANDREIA SILVA – Dendritic cell exosomes are rich in chemotactic mediators and recruit mesenchymal stem/ stromal cells

KIM VARMING – Female patients with suspected adverse effects of vaccination against human papilloma virus, have a different composition of extracellular vesicles in plasma compared with a healthy female control group

SARAH CRAIN – Canine Wharton’s Jelly mesenchymal stem cell suppression of T helper cell division is mediated by exosomes

ROSSELLA CRESCITELLI – Extracellular vesicle miRNA 371-5p is a biomarker candidate in isolated hepatic perfusion from patients with metastatic uveal melanoma

Chairs: Margot Zöller, Malene M. Jørgensen




7:45 – 8:00AM MEET THE EXPERTS

Room: Willem Burger MEET THE EXPERTS SESSION 4 EVs as Modulators of Drug Resistance and Tumor Metastasis

Speakers: Janusz Rak, Alissa Weaver

Moderator: Lorraine O'Driscoll

Room: Jurriaanse MEET THE EXPERTS SESSION 5 EVs – Associated Versus Soluble Functional Molecules

Speakers: Aled Clayton, Guillaume van Niel

Moderator: María Yáñez-Mó

Room: van Weelde MEET THE EXPERTS SESSION 6 Extracellular RNAs: EVs or Not EVs

Speakers: Andrey Turchinovich, D. Michiel Pegtel

Moderator: Jan Lötvall

ORAL SESSIONS

9:00 – 10:15AM SYMPOSIUM SESSION 19Room: Jurriaanse EVs in the Immune System

Speakers: SONI SANOOJ – Macrophages preferentially package TNF within microvesicles in response to ATP

TOM GROOT KORMELINK – Extracellular vesicles rapidly and massively released from degranulating mast cells are enriched in distinct phospholipid species and contain active carboxypeptidase

ANDREW DEVITT – Apoptotic Cell-derived Extracellular Vesicles (ACdEV) – a matter of life and death.

SUSANNE VAN DER GREIN – Vitamin D3-tolerized dendritic cells release extracellular vesicles that suppress proinflammatory cytokine production

MARTA MONGUIÓ-TORTAJADA – Polyclonal T cell proliferation is inhibited by Umbilical Cord MSC-derived EVs but not by MSC conditioned media

Chairs: Jan Lotvall, Esther Nolte-‘t Hoen

Room: Willem Burger SYMPOSIUM SESSION 20 EVs in Cancer Metastasis I

Speakers: MARJOLEIN VAN DRIEL – Positive effects of vitamin D in bone metastases: role for extracellular vesicles?

EMERENCE CROMPOT – Role of bone marrow-mesenchymal stromal cell derived extracellular vesicles on Chronic Lymphocytic Leukemia B cells defined by functional studies and microarray analysis

MARGOT ZOELLER – Pancreatic cancer initiating cell and exosome CD44v6 affects additional stem cell marker expression and cancer stem cell activities

CAROLINA F RUIVO – Exosomes mediate intra-tumor communication in pancreatic cancer

AIJA LINE – Hypoxic cancer-derived EVs contain specific miRNA cargo and mediate intercellular communication among hypoxic cancer cells

Chairs: Dolores Di Vizio, Suresh Mathivanan

Room: van Weelde SYMPOSIUM SESSION 21 EVs in Reproduction and Pregnancy

ISEV2016 ANNUAL MEETING6:30-8:30PM SATELLITE MEETING Room: Jurriaanse ISEV-ISAC-ISTH EV flow cytometry work group

This satellite event will provide insight into the scientific interaction of three big international societies, and will also give opportunity for young researchers to present the first results of this collaboration.

Chairs: Joanne Lannigan, Marca Wauben

6:30-6:35: MARCA WAUBEN – Introduction of the ISEV- ISAC-ISTH EV flow cytometry work group

6:35-6:45: LEONIE DE ROND – Size and refractive index determination to standardize vesicle flow cytometry

6:45-6:55: STEN LIBREGTS – Interference of non- fluorescent particles in fluorescence EV measurements:

6:55-7:10: RIENK NIEUWLAND – Update on ISTH EV flow cytometry workgroup studies (scatter-based measurements)

7:10-7:35: JOHN NOLAN – Standards and calibration in flow cytometry & workgroup Spring 2016 activities

7:35-7:45: JOANNE LANNIGAN – Update on the ISAC MV IDG workshop

7:45-8:30: Discussion about next steps

FRIDAY, 6 MAY 2016


Speakers: NAVIND JAYASOORIAH – Extracellular vesicles – the next small thing in epigenetic inheritance

JULIANO DA SILVEIRA – Extracellular vesicles contents alter RNA profile after in vitro treatment of bovine cumulus-oocyte-complex

AMAL AL-DOSSARY – Functional Impact of Delivery of Oviductosomal PMCA4 to Pmca4 Null Sperm in Vitro

BETH HOLDER – Exosome trafficking from immune cells to the placenta: a novel mode of maternal-placental messaging

ANIRBAN BANERJEE – Extracellular vesicles produced by group B streptococcus disrupts feto-placental barrier and leads to preterm birth

Chairs: Chris Gardiner, Paul Harrison


10:45 – 11:25AM PLENARY SESSION 3Room: Willem Burger EVs in Cell Biology

Speaker: FRANCISCO SÁNCHEZ-MADRID PH.D. is Professor at the Universidad Autónoma de Madrid and is Head of the Immunology Department in the La Princesa Hospital in Madrid, Spain. - Immune Cell-to-Cell communication: Mechanisms of microRNA and proteins sorting into exosomesChairs: Clotilde Thery, Dirk Michiel Pegtel

11:25 – 12:30PM ISEV GENERAL ASSEMBLYRoom: Willem Burger

12:30 – 1:30PM LUNCH

Room: Schadee JEV EDITORIAL BOARD MEETING

1:30 – 2:45PM ORAL SESSIONSRoom: van Weelde SYMPOSIUM SESSION 22 EVs in Acute and Chronic Inflammatory Disease

Speakers: NIALA MASOUMI – Circulating EBV-modified exosomes in SLE patients target the renal tubular epithelium delivering inflammatory EBER1 and EBV-microRNAs

HEEGAARD NIELS H. H. – Molecular pathology of circulating extracellular vesicles in systemic lupus erythematosus (luposomes) provides novel insight into disease mechanisms

TSUKASA KADOTA – Suppression of autophagy by extracellular vesicles promotes myofibroblast differentiation in COPD pathogenesis

GYONGYI SZABO – Extracellular vesicles and their microRNA cargos are novel biomarkers and intercellular communicators in alcoholic liver disease

MERCEDES TKACH – Subtypes of extracellular vesicles released by dendritic cells promote differential CD4+T cell responses

Chairs: Philip Askenase, Muthuvel Jayachandran

Room: Jurriaanse SYMPOSIUM SESSION 23 EVs in Cancer Metastasis II

Speakers: SERENA RUBINA BAGLIO – Osteosarcoma exosomes establish a prometastatic inflammatory loop by engaging mesenchymal stem cells

PEINADO HECTOR – Role of tumor-seceted exosomes reprogramming the lymph node microenvironment during metastasis

SCHILLACI ODESSA – Metastatic colon cancer extracellular vesicles spread malignant properties in tumor microenvironment affecting the behavior of both tumor and endothelial cells

JIE HU – Exosome-mediated delivery of miR-26a suppresses liver cancer growth in vitro and in vivo

NAOHIRO SEO – CD8+ T cells interrupt tumor progression including invasion and metastasis by exosome-mediated depletion of mesenchymal tumor stromal cells

Chairs: Richard Simpson, Hector Peinado

Room: Willem Burger SYMPOSIUM SESSION 24 EVs in Immune Modulation in Bacterial and Parasitic Infections

Speakers: LORENA MARTIN-JAULAR – Spleen-dependent immune protection elicited by reticulocyte-derived exosomes from malaria infection revealed by T cell population changes

NETA REGEV-RUDZKI – Malaria parasites regulate secretion of exosomes carrying distinct cargo

GILLIAN COAKLEY – Immunomodulation of anti-parasitic responses via Heligmosomoides polygyrus secreted exosomes

JE CHUL LEE – Worsening of atopic dermatitis by Staphylococcus aureus-derived membrane vesicles

ANNA LENA JUNG – Legionella pneumophila outer membrane vesicles are potent pro-inflammatory stimulators for macrophages

Chairs: Hernando del Portillo, Cherie Blenkiron




3:15 – 4:15PM DIAMOND SPONSOR SESSION: Room: Willem Burger Caris Life Sciences

Speaker: MARK R. MIGLARESE – Adaptive Dynamic Artificial Poly-ligand Targeting (ADAPT) of Plasma Exosomes: a Highly Multiplexed Non-Invasive Diagnostic Discovery Platform

Chairs: Aled Clayton, Marca Wauben

4:15 – 5:00PM ORAL SESSIONSRoom: Jurriaanse SYMPOSIUM SESSION 25 EVs Viruses and Viral Infections

Speakers: SHILPA BUCH – MiR-9 mediated synaptodendritic alterations in HAND: Role of extracellular vesicles

DIRK MICHIEL PEGTEL – Sensing of latent EBV infection through exosomal transfer of 5’pppRNA

ANDREA D. LIPINSKA – Exosomal incorporation but not interaction with major histocompatibility class II (MHC II) molecules is a conserved feature of alphaherpesvirus glycoprotein B

Chairs: Jennifer Jones, Matias Ostrowski

Room: van Weelde SYMPOSIUM SESSION 26 EVs in Cellular Differentiation and Development

Speakers: XIAOQIN WANG – Osteogenic differentiation of human mesenchymal stem cells using exosomes derived from mesenchymal stem cells undergoing osteogenic differentiation

NISA RENAULT – Human iPS-derived cardiac progenitors secrete cardioprotective extracellular vesicles

MARIJKE ZONNEVELD – Breast milk-derived extracellular vesicles influence the development of the epithelial and immunological gastrointestinal barrier

Chairs: Eva Rohde, Marjolein van Driel

Room: Willem Burger SYMPOSIUM SESSION 27 Cell Biology of EVs II

Speakers: ALISSA WEAVER – KRAS signaling regulates Argonaute 2 sorting into exosomes

PASCALE ZIMMERMANN – Syntenin exosomes mediate SRC function in cell-cell communication

NICOLE MEISNER-KOBER – Exosomes surf on filopodia to enter cells at endocytic hot spots, and shuttle with endosomes to scan the endoplasmic reticulum – a highway to the cell.

Chairs: Bernd Giebel, Guillaume van Niel


Room: Willem Burger Foyer POSTER SESSION 1 Non-cancer Biomarkers

AGNIESZKA KAMINSKA – Urinary extracellular vesicles number and diameter as biomarkers of renal disease in diabetic patients

JOHAN SKOG – Discovery of a Urinary exosome mRNA signature for the diagnosis of human kidney transplant rejection

CIRO TETTA – The content of microRNA is modulated in plasma extracellular vesicles in patients with end-stage chronic kidney disease

BAS VAN BALKOM – RNA profiles in exosomes from kidney transplantation preservation fluid are associated with post transplantation kidney function

HERNANDO A. DEL PORTILLO – Detection of Plasmodium vivax, Trypanosoma cruzi and Fasciola hepatica proteins by extensive proteomic analysis in plasma-derived exosomes

INGRID BANK – A plasma extracellular vesicle protein signature as a biomarker for diagnosis of unstable angina

EREZ EITAN – Impact of Age on Plasma Extracellular Vesicle Concentration and Protein Content in Humans

KRISZTIAN KVELL – Combined use of exosome miRNA, TREC and thymulin values as circulating biomarkers of immune senescence

BENEDIKT KIRCHNER – Exosomal isoforms of microRNAs as a novel group of tissue-specific biomarkers

JOERI LAMBRECHT – Comparison of circulating exosomal miRNAs during the process of liver fibrosis by chronic hepatitis B and C infection

JAMES BOWNESS – The utility of exosomes as a biomarker of N-methyl-D-aspartate receptor encephalitis

MORTEN HJULER NIELSEN – Skeletal muscle-derived microvesicles expressing fatty acid transporters are increased by acute exercise

GESIELE VERISSIMO – Extracellular vesicles from lung epithelial cells as early indicator of paraquat exposure

Chairs: Malene Jørgensen, Joanne Welton

Room: Willem Burger Foyer POSTER SESSION 2 EVs and Cancer

MARIA HARMATI – Induced cell damage altered proteome and miRNA profile of melanoma exosomes and interactions with tumor stroma cells

RADHIKA POCHAMPALLY – Extracellular vesicles from Mesenchymal stromal/stem cells transfer miRNAs 205 and 31 and inhibit breast cancer metastasis by regulating UBE2N/Ubc13



LUANA LUGINI – Exosomes from human colorectal cancer induce a tumour-like behavior in colonic mesenchymal stromal cells

MATEJA MANCEK KEBER – EV transfer of the proinflammatory signal from cells carrying lymphoma-associated MyD88 mutation

DAVE CARTER – The role of extracellular vesicles in the intercellular response to stress

DOMINIK BUSCHMANN – The volatile anesthetic agent sevoflurane influences cancer-related miRNAs in circulating extracellular nanovesicles

CELINE BOUVY – Transfer of multidrug resistance between leukemia cells via extracellular vesicles

GIULIA CESI – Detection, characterization and function of extracellular vesicles in resistant melanoma

GOZDE GUCLULER – D-type CpG oligonucleotide encapsulating and antigen harboring exosomes improve therapy of tumors

FARRUKH AQIL – Oral delivery of the chemotherapeutic drug, paclitaxel via bovine milk exosomes

Chairs: Janusz Rak, Valentina Minciacchi

Room: Willem Burger Foyer POSTER SESSION 3 EVs and the Immune System

TOM DRIEDONKS – Immunogenic and tolerogenic dendritic cells release extracellular vesicles that differ in small noncoding RNA content

SIM TUNG – Exosomes released by regulatory T cells modulates T cell polarisation

PHIL ASKENASE – An Antigen Specific Exosome Cascade Leads to Final Antigen Presenting Cell-Derived Peptide-MHC-Specific Exosomes That Suppress the Effector T cells

KRISZTINA V. VUKMAN – Immunomodulatory effect of mast cell-derived extracellular vesicles

GABRIEL DOSTERT – Evaluation of the CD73 transfer into NK cells by exosomes derived from endothelial cells and its immunomodulatory effect

ANNE LOUISE SCHACHT REVENFELD – Contact-dependent and –independent co-cultures of allogeneic human immune cells yield heterogeneous vesicle phenotype but conserved cellular phenotype

STEFFI BOSCH – Immune-stimulatory properties of serum exosomes from (pre-) diabetic mice

LOTTE PUGHOLM – Phenotyping of leukocytes and leukocyte-derived extracellular vesicles

ÁRPÁD FERENC KOVÁCS – Impact of preeclampsia-associated extracellular vesicles on the monocyte-macrophage system: an in vitro analysis

CHEUK-LUN LEE – Placenta-derived extracellular vesicles induce differentiation of monocyte into decidual macrophage-like phenotype

Chairs: Paul Robbins, Tom Groot Kormelink

Room: Willem Burger Foyer POSTER SESSION 4 EVs in the Tumor Microenvironment

SARA ROCHA – Gastric cancer cell-derived extracellular vesicles modulate E-cadherin expression, invasion and migration of normal cells from the tumor microenvironment

YUTAKA NAITO – Functional analysis of extracellular vesicles in scirrhous type gastric cancer microenvironment

DIMITRIOS CHIOUREAS – The role of anaplastic large cell lymphoma-derived exosomes in the tumor microenvironment

PAULINA PODSZYWALOW-BARTNICKA – Stress response signaling in leukemia cells modifies composition of proteases in extracellular vesicles what supports invasion

ANNIE REBIBO-SABBAH – Role of Extracellular Vesicles in Acute Myeloid Leukemia evolvement

ANAT AHARON – The Role of Microvesicles in Multiple Myeloma Progression

WEI-LUN HUANG – The role of lung cancer derived extracellular vesicles in tumor progression

GABRIELLA DOBRA – Stressors alter intercellular communication and exosome profile in nasopharyngeal carcinoma cells

MARIANNA DI FRANCESCO – EVs from human ovarian cancer drive the normal fibroblasts shift to “cancer associated fibroblasts”-like phenotype

CHRISTIAN BRØGGER STOLBERG – GBM Extracellular Vesicles: Mediators of Invasion and Brain Stromal Modulation

SOPHIE BAUMGART – Exosomal miRNAs play an important role in cell-cell communication between bladder cancer cells and tumor microenvironment

ALIREZA LABANI MOTLAGH – Differential Expression of ligands for NKG2D and DNAM-1 receptors by epithelial ovarian cancer-derived exosomes and their influence on NK cell cytotoxicity

MARK OFIELD – The role of extracellular vesicle-mediated signalling in the oral cancer microenvironment

FEDERICA CALAPA – Modulation of exosomes secretion during NaBu induced differentiation of HT29 colon cancer cells, and effects of exosomes administration on normal and cancer cells



KAI HÄRKÖNEN – HAS3 overexpression induces secretion of diverse EVs by tumor cells

Chairs: Pilar Martin-Duque, Jason Webber

Room: Van Der Mandele POSTER SESSION 5 and Arcadis Zaals MSCs & Tissue Regeneration and Morphogenesis

SICHENG WEN – Biological Effects of Different Extracellular Vesicles Population on Reversal of Marrow Cells Radiation Damage

MAGDALENA LORENOWICZ – MSC-derived extracellular vesicles- towards a novel therapy for osteoarthritis

BERND GIEBEL – Immunomodulatory Impact of Mesenchymal Stem Cell-derived Extracellular Vesicles on Peripheral Blood Cells in vitro

KIRSI RILLA – Hyaluronan-coated extracellular vesicles are secreted by human mesenchymal stem cells

FABIANA GERACI – Paracrine effect of membrane vesicles released by mouse mesoangioblast stem cells

SUKHBIR KAUR – CD47 Regulates the Reprogramming of Endothelial Cells by Breast Cancer Stem Cell-Derived Extracellular Vesicles

MARTA ADAMIAK – Extracellular vesicles released by induced pluripotent stem cells modulate cellular function of recipient cardiac endothelial cells

THOMAS DRAEBING – Extracellular vesicle-delivered BMP: A novel paracrine mechanism during heart development

SOPHIE ROME – Depleting Extracellular vesicles from fetal bovine serum affects muscle cell proliferation and differentiation

CHRISTIEN BEEZ – Extracellular vesicles (EVs) from human cardiac-derived adherent proliferating cells (CardAPs) – a comparative characterization

JUNHO KIM – Hematopoietic differentiation of embryonic stem cells by stromal cell-derived nanovesicles

WONJU JO – The Effect of Exosome-Mimetic-Nanovesicles on Mesenchymal Stem Cell Proliferation

KARIN PACHLER – RNA profiling of naive and differentiated MSC-derived EV: Towards miRNA-based release criteria for future therapeutic application of EV in clinical trials

Chairs: Magdalena Lorenowicz, Eva Rohde

Room: Van Der Mandele POSTER SESSION 3 and Arcadis Zaals Novel Technical Developments in EV Characterization

VASILIS TOXAVIDIS – Characterization of EVs by Flow Cytometry

ELMAR GOOL – Optimized staining protocol for extracellular vesicle characterization by dedicated flow cytometry

JENS B. SIMONSEN – Flow cytometry for determining size distribution of microvesicles and variation in the lipid composition of individual liposomes

MARÍA YÁÑEZ-MÓ – Development of a bead-assisted flow cytometry method for the semi-quantitative analysis of EVs

L DE ROND – Label-free detection of extracellular vesicles in human breast milk compared to infant formula

PAULINE CARNELL-MORRIS – Using F-NTA for characterizing exosomes

GYLLION LOOZEN – Simulation study of optical trapping of vesicles with a dual-waveguide trap

MARTIN E.M. PARSONS – Optimal EV measurement in complex biological samples using nanoparticle tracking analysis

DEBORAH L. M. RUPERT – Physical characterization of extracellular vesicles using surface-based sensing

SILVIA PICCIOLINI – Use of Surface Plasmon Resonance and Raman Spectroscopy for the detection and characterization of exosomes

AGNES REINER – Combined lipid and protein analysis of EVs with surface plasmon-enhanced fluorescence spectroscopy

TAKANORI ICHIKI – A microfluidic chip system for profiling individual extracellular vesicles

ROCCO LUCERO – Cloud-hosted tools for the analysis of extracellular RNA and the construction of the exRNA Atlas

ROZOWSKY JOEL – Developing Quality Control Metrics for Extracellular RNA Communications Consortium Data

EDWIN VAN DER POL – Size and refractive index determination by flow cytometry to discriminate between extracellular vesicles and lipoprotein particles in human plasma

SAMI VALKONEN – Biological reference materials to standardize measurements of extracellular vesicles

Chairs: Uta Erdbrugger, Sten Libregts

Room: Van Der Mandele POSTER SESSION 7 and Arcadis Zaals Late breaking Poster Session 2

KSENIA MAXIMOVA – On-chip biodetection, analysis and nanometrology of platelet-derived vesicles

JUNHONG YAN – Surface protein profiling on exosomes by proximity barcoding assay

UTA ERDBRUEGGER – Search for the optimal dye for EV characterization

CASEY MAGUIRE – Rapid isolation of intact exosomes and extracellular vesicles




8:00 – 9:00AM EXPERTS MEETNew to this year, ISEV introduces the "Experts Meet" sessions during which the interactive discussions will focus on specific biofluids.

Room: Willem Burger EXPERTS MEET SESSION 1 Biofluids: Blood Moderator: Kenneth W. Witwer

Room: van Weelde EXPERTS MEET SESSION 2 Biofluids: Milk Moderator: Marca Wauben

Room: Ruys, EXPERTS MEET SESSION 3 van Rijckevorsel, - Biofluids: Urine Plate Zaals Moderator: Guido Jenster

9:00 – 10:00AM LATE BREAKING ORAL SESSIONS

Room: Willem Burger LATE BREAKING SESSION 1

Speakers: LINDA RIKKERT– Cancer-ID: strategies to identify rare extracellular vesicles in human body fluids

JORDI BERENGUER – Chemokine receptor targeting inhibits EV uptake and EV-induced phenotype

ERZSÉBET LIGETI - Central role of the beta2 integrin Mac1 (CD18) in activated EV-generation in murine neutrophilic granulocytes

TOM KEULERS – GABARAPL1 is required for hypoxia-induced extracellular vesicle secretion

Chairs: Lydia Alvarez-Erviti, Theocharis Panaretakis

Room: van Weelde LATE BREAKING SESSION 2

Speakers: JAN VAN DEUN – EV-TRAQ: Tracking transparent reporting and quality to boost standardized extracellular vesicle research

GLENN VERGAUWEN – A combination of size-exclusion chromatography with density gradient centrifugation to purify EVs from plasma

ISEV2016 ANNUAL MEETINGYUHUA FARNELL – Comparative analyses of differential miRNA expression profiles between extracellular and intracellular components of SCN2.2 cells

JANNEKE BOERE – Increased recovery of CD44+ extracellular vesicles from synovial fluid of healthy joints by incubation with hyaluronidase prior to isolation

MARIA STENUM HANSEN – Biological properties of extracellular vesicles in bovine milk

JIWON HONG – First proteomic profiling of exosomes in rodent intestinal lymph

KOSGODAGE UCHINI – Non-phagocytic epithelial cells take up microvesicles by macropinocytosis

MANUELA BASSO – Characterization of RNA contained in extracellular vesicles released by primary astrocyte cultures

XUEWEI ZHAO - Proteomics and miRNA Analysis of Exosomes Derived from Differentiating Neuronal Cells

FUMIYASU MOMOSE – Cytotoxic miRNAs against the cultured mesenchymal stem cells are included in human T cell-derived exosomes

BOAH VANG – Automated extracellular vesicle production and concentration from bone marrow-derived mesenchymal stem cells

PIERRE-YVES MANTEL – Extracellular vesicles in the regulation of host-pathogen interactions during malaria

EWA BIELSKA – The role of extracellular vesicles in pathogenesis of the human pathogen Cryptococcus gattii

MUHAMMAD NAWAZ – Profiling and characterization of embryo and seminal fluid extracellular vesicle-associated microRNAs in Sus scrofa: a possible role in differentiation and developmental communication processes

Chairs: Theoharis Panaretakis, Alicia Llorente

7:30PM NETWORKING EVENT Registration required

SATURDAY, 7 MAY 2016


SERGEI G. KRUGLIK – Raman tweezers microspectroscopy of extracellular vesicles: a pilot study on nucleic acids detection and characterization

BARBORA KONECNÁ – A Mathematical Approach to Understand the Generation of Multivesicular Bodies

Chairs: Yves DeClerck, Felix Royo

Room: Ruys, van Rijckevorsel,Plate Zaals LATE BREAKING SESSION 3

Speakers: KYRA DEFOURNY – Assessing the role of extracellular vesicle enclosure as non-lytic egress route for mengovirus

OHAD YOGEV – Kaposi's sarcoma-associated herpesvirus manipulates exosomes secretion to regulate its microenvironment metabolism

YIJUN YANG – Exosome from Plasma of Plasmodium-infected Host Expressing MicroRNAs Inhibit Angiogenesis in A Murine Lewis Lung Cancer Model

PHIL ASKENASE – Spinal Cord Injury Recovery Mediated by Exosomes from Mesenchymal Stem Cells (MSC)

Chairs: David Carter, Jason Webber


FEATURED ABSTRACTS

10:30 – 11:50AM PLENARY SESSION 4Room: Willem Burger ISEV2016 Featured Abstracts

CECILIA LÄSSER – The First Massive Open Online Course (MOOC) on EVs

Featured abstract presenters:

BENJAMIN SCICLUNA – Tracking exosomal miRNA biomarkers in prion disease

HEFIN JONES – Neutrophil-derived microvesicles enter cartilage and protect the joint in inflammatory arthritis

KATHARINA BEER – PAD-1 activates TAT-5 to prevent extracellular vesicle release in C. elegans embryos

Chairs: Guillaume Van Niel, Edit I Buzás and María Yáñez-Mó

11:50 – 12:35PM CLOSING SESSIONSRoom: Willem Burger

POSTER AWARDS

ORAL AWARDS

SPECIAL ACHIEVEMENT AWARD

SCIENCE WRAP-UP SESSION: ALICIA WEAVER

CLINICAL WRAP-UP SESSION: PETER QUESENBERRY

12:35 – 12:45PM ISEV2017 ANNOUNCEMENT

CLOSING REMARKS



NETWORKING EVENT

Friday, 6 May 2016 • 7:30 pmRegistration required

THE GROTE OF SINT-LAURENSKERT (LAURENS CHURCH)

The ISEV2016 Networking Event will be held in the relaxing ambiance of a beautiful church, with a buffet dinner and dancing. This event provides a great opportunity to meet the leaders and young researchers in the field of Extracellular Vesicles while both strengthening existing and initiating new collaborations. Expand your circle of colleagues and discover the latest news and insights into the research of your peers!

Within easy walking distance of the De Doelen Convention Centre, this Rotterdam Heritage Site, built between 1449 and 1525, is the only late-Gothic monument from the Middle Ages in the city.

31


ROTTERDAM, THE NETHERLANDS

Rotterdam is the second-largest city in the Netherlands with over 600,000 and currently one of the largest ports in the world. As a result of international trade, Rotterdam has an intercultural, cosmopolitan character with over 170 nationalities. It is a young, dynamic, international city with a passionately beating heart. The city keeps reinventing itself at a rapid pace while continuously redefining and expanding its already impressive skyline. Rotterdam is simply bursting with architecture, design, art and culture, but also boasts a wide and varied range of shops, attractions, festivals, restaurants and parks.

VENUE

The de Doelen International Congress CentreRotterdam, The Netherlands

The de Doelen International Congress Centre is located at the very

heart of the dynamic city of Rotterdam and its special appeal lies in its dual function as international concert hall and conference centre. This is underscored by the building’s design: the remarkable 1960’s functionalist architecture of the original concert hall contrasts beautifully with the venue’s more dynamic and modern design.

ACCOMMODATIONS

We have reserved a number of rooms in various hotels that have been carefully selected for your convenience. The majority of the hotels are located in the center of Rotterdam, near the congress venue de Doelen, or at a location from which the congress venue can easily be reached by public transportation.

Reservations can be made online with the individual hotels.

Hotel Category Address Distance to de DoelenThe Manhattan Hotel

Rotterdam5 Weena 686

3012 CN Rotterdam3 minute walk

Hilton Rotterdam 5 Weena 10 3012 CM Rotterdam

7 minute walk

NHow Rotterdam 4 Wilhelminakade 137 3072 AP Rotterdam

15 minutes by public transport

NH Atlanta Rotterdam 4 Coolsingel/Aert van Nesstraat 4 3012 CA Rotterdam

6 minute walk

Holiday Inn Rotterdam – Central Station

3 Weena 121 3013 CK Rotterdam

7 minute walk

SEE YOU IN ROTTERDAM!

www.isev.org

Annual Meeting ISEV2016 - cdn.ymaws.com · Poster Session 1 - Cell Biology of EVs: Biogenesis and...

Documents

Transcript of Annual Meeting ISEV2016 - cdn.ymaws.com · Poster Session 1 - Cell Biology of EVs: Biogenesis and...