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Announcements
● Tutoring CenterSCI I, 407M 12-3, 5:30-6:30; W 8-9, 5:30-6:30, Th 8-12, 6-7; F 8-9
● MasteringBiology Assignment due Tuesday 5/24●Today’s Lecture: parts of Chapters 11-12
Biotechnology
Biotechnology:Is the manipulation of organisms or their components to make useful products
Biotechnology today means the use of DNA technology, methods for:
•Studying and manipulating genetic material•Modifying specific genes •Moving genes between organisms
BiotechnologyClone – from the Greek “Klon” meaning genetic duplicate
•Reproductive cloning
•Therapeutic cloning
•Recombinant DNA Technology (or DNA cloning)
Reproductive Cloning
(a) The first cloned cat (b) Cloning for medical use
Gray wolfGaurBantengMouflon calfwith mother
Human Cloning–Cloning of animals
●Has heightened speculation about human cloning ●Is very difficult and inefficient
–Critics raise practical and ethical objections to human cloning.
Concept Check: Clicker Question•Nuclear transplantation experiments provide strong evidence for which of the following?
A. Differentiated vertebrate cells still maintain their full complement of DNA.B. Differentiated vertebrate cells do not maintain their full complement of DNA. C. Vertebrate cloning is not feasible.D. Cell differentiation is an irreversible process.
Biotechnology–The purpose of therapeutic cloning is not to produce a viable organism but to produce embryonic stem cells.
–Embryonic stem cells (ES cells)•Are derived from blastocysts •Can give rise to specific types of differentiated cells
BiotechnologyAdult stem cells•Are cells in adult tissues •Generate replacements for nondividing differentiated cells
Adult stemcells in
bone marrow
Culturedembryonic stem cells
Different cultureconditions
Different types ofdifferentiated cells
Heart muscle cells
Nerve cells
Blood cells
Biology and Society●Embryonic stem cells are currently derived from extra human blastocysts that sometimes result from in vitro fertilization techniques. From 2001 to 2009, stem cell research supported by U.S. agencies was restricted to only a small number of stem cell lines. (Meanwhile, other countries were going ahead with embryonic stem cell research.) But in March 2009, President Obama signed an executive order reversing federal opposition to embryonic stem cell research.
•Do you support the current U.S. governmental policy on stem cell research?
StronglyAgree
StronglyDisagreeA. E.C.B. D.
Recombinant DNA technologyDNA isolated from
two sources and cut by the same enzyme
Gene of interest
RecombinantDNA
Plasmid(vector)
Transgenic organisms
Useful products
Gene for pestresistance
Gene fortoxic-cleanup
bacteria
Some uses of proteins:
Protein for“stone-washing”
jeans
Protein fordissolving
clots
Some uses of genes:
Examples:In 1982, the world’s first genetically engineered pharmaceutical product was sold.
Humulin, human insulin:●Was produced by genetically modified bacteria●Was the first recombinant DNA drug approved by the FDA
Genetically Modified (GM) FoodsIn the United States today, roughly one-half of the corn crop and over three-quarters of the soybean and cotton crops are genetically modified.
●Corn has been genetically modified to resist insect
infestation.
Palmiter et al. (1982) Dramatic growth of mice that develop from eggs microinjected with metallothionine-growth hormone fusion genes. Nature 300: 611-615
“Supermice”
Other Examples:
DNA PROFILING AND FORENSIC SCIENCE
DNA profiling:
•Can be used to determine if two samples of genetic material are from a particular individual
•Has rapidly revolutionized the field of forensics, the scientific analysis of evidence from crime scenes
Investigating Murder, Paternity, and Ancient DNA
DNA profiling can be used to:
•Test the guilt of suspected criminals•Identify tissue samples of victims•Resolve paternity cases•Identify contraband animal products•Trace the evolutionary history of organisms
DNA PROFILING AND FORENSIC SCIENCE
DNA isolated
DNA amplified
DNA compared
Crime scene Suspect 1 Suspect 2
●To produce a DNA profile,
scientists compare genetic markers, sequences in the genome that vary
from person to person.
Short Tandem Repeat (STR) AnalysisRepetitive DNA:
•Makes up much of the DNA that lies between genes in humans•Consists of nucleotide sequences that are present in multiple copies in the genome
Crime scene DNA
Suspect’s DNA
Same number ofshort tandem repeats
Different numbers ofshort tandem repeats
STR site 1 STR site 2
AGAT
AGAT GATA
GATA
Amplifiedcrime sceneDNA
Amplifiedsuspect’sDNA
Longerfragments
Shorterfragments
Short Tandem Repeat (STR) Analysis
GENOMICS
Genomics is the science of studying complete sets of genes (genomes).
●The first targets of genomics were bacteria.●As of 2009, the genomes of nearly one thousand species have been published, including:
Baker’s yeastMiceFruit fliesRice
The Human Genome ProjectBegun in 1990, the Human Genome Project was a massive scientific endeavor:
● To determine the nucleotide sequence of all the DNA in the human genome and ● To identify the location and sequence of every gene
At the completion of the project in 2004:●Over 99% of the genome had been determined to 99.999% accuracy● 3.2 billion nucleotide pairs were identified● About 21,000 genes were found● About 98% of the human DNA was identified as noncoding