ANNEXURE 1 8. i: Facilities under the Odisha Sickle Cell Project (NHM Odisha):

All the services are provided free of cost

Sl Service Facility detail Service detail Availability (time/day)

NODAL CENTRE, VIMSAR BURLA

OPD

OPD registration Clinical evaluation Patient data recording Case sheet update Advice for laboratory tests Prescription of Folic acid & Hydroxyurea

Every individual attending to the sickle cell OPD is assigned a unique registration number. Each individual is clinically evaluated for sign and symptom of sickle cell disease. All laboratory tests adviced are updated in the concerned case sheet /files. Family screening is done wherever indicated. All necessary clinical and laboratory findings are entered as a soft copy of patients data. Appropriate dose of Hydroxyurea is prescribed wherever indicated. Adequate counseling to sickle cell traits and sickle cell disease patients and family member are offered.

All working days from 9:00 AM to 5:00 PM (lunch 1:00 to 2:00)

Diagnostic

Basic screening, hematology Biochemistry Advanced screening & confirmation

Sickling test (mandatory) microscopy, Alkaline electrophoresis on agarose gel pH 8.6 (Mandatory), Cation exchange High Performace Liquid Chromatography (HPLC, BioRad Variant II), complete Blood Count (Sysmex KX21, Transasia), Full panel biochemistry assays (Cobas Integra 400+, Roche India).

All working days from 9:00 AM to 5:00 PM (lunch 1:00 to 2:00)

Molecular evaluation

PCR Gene Sequencing (Whenever necessary)

PCR technology based DNA analysis (ARMS, GAP, RE), RFLP studies wherever required using DNA PCR method, Gel documentation and post PCR facility, DNA genome sequencing facility for selected samples (under collaboration with Anthropological Survey of India, Govt of India). All rqeuierd laboratory set up/equipments/instruments/wares necessary for molecular biology laboratory is available.

All working days from 9:00 AM to 5:00 PM (lunch 1:00 to 2:00)

Others Issuing certificates Certificates to Sickle cell patients are

issued on request for availing benefits provided under different government schemes.

Satelite units (Sickle cell unit at District headquarters hospital):-

12 such units are functioning in district of Sambalpur, Balangir, Bargarh, Jharsuguda,

Sundargarh, Deogarh, Angul,Boudh, Kandhamal,Kalahandi, Nuapada,Sonepur.Each unit is

managed by one Program Associate and one Laboratory Technician.

Various services provided at sickle cell district units incluiding screening for sickle cell

hemoglobinopathy by sickle slide test & Hb electrophoresis ,registration of sickle cell trait and

sickle cell disease,providing counseling and referring to nodal centre.

Health Camps are organized according to targets given by NHM in each year.Last year (2016-17)

residential school screening for sickle cell hemoglobinopathy in 12 project districts were

conducted in collaboration with RBSK programme and 12511 no of children were screened.

ANNEXURE 2

8. ii: Completed projects under Sickle cell Unit: A. Research Projects:

Completed 1. Title: Study of morbidity pattern in sickle cell disease in Western Orissa and its correlation with

fetal hemoglobin concentration and different epistatic factors like malaria. Sponsored by Department of Biotechnology, Government of India, New Delhi Status – Completed (2007-2010)

2. Incidence and severity of vaso occlusive crisis in Sickle Cell Disease patients of Western Orissa and its correlation with various genetic markers like beta globin gene cluster haplotype, alpha thalassemia and XmnI polymorphism. ICMR sponsored Project. No. 45/7/2008-Hae/BMS date. 10/2/2009 (2009-2011). Dr. Ranjeet Singh Mashon, S.R.F.

3. Title: Influence of heterozygous and Homozygous alpha thalassemia on the severity of Plasmodium falciparum malaria in India”. Sponsored by Department of Science and Technology, New Delhi. F. No./SR/SO/HS-140/2007, dated 09.11.2009 Status – Completed (2010-2015).

4. Influence of Sickle gene and alpha thalassaemia on the severity of Plasmodium falciparum malaria in Eastern India. ICMR sponsored project, IRIS ID 2012-13400 (2013-2016). S.R.F.

Ongoing 5. Title: Odisha Sickle Cell Project

Under Odisha Health Sector Plan (OHSP) Sponsored by National Rural Health Mission, Odisha and Odhsia Health Sector Plan, Odisha. Status – Ongoing (2010-)

6. Title: Epinephrine induced alterations in the erythrocyte influence occurrence of frequent vasso occlusive crisis in Sickle Cell Disease. Sponsored by Department of Biotechnology, Govt of India, New Delhi.DBT Project No. – BT/BIO-CARe/075512012, dtd. 21.10.2014. Status – Approved (2015-2018)

Collaboration projects:

A Collaboration project is undertaken between the Anthropological Survey of India, Ministry of Culture, Govt. Of India and Odisha Sickle Cell Project (NHM Odisha), V. S. S. Institute of Medical Sciences And Research, Burla Odisha, titled “Genetic study of inherited haemoglobin disorders in western Odisha under the Odisha Sickle Cell Project (NHM Odisha)” vide sanction letter F No. 18-22/PMI/2011 dated 15.06.2013 of the Anthropological Survey of India for three years. The project focuses on genotyping of Haemoglobinopathy in western Odisha by DNA sequencing of samples under Odisha Sickle Cell Project (NHM Odisha) under the project.

B. Post Graduate Students projects:

Batch-I (6 months): 1. Monalisa Rana, M.Sc. Biotech student, Sambalpur University (Influence of alpha thalassaemia

on the clinical severity of Sickle Cell Disease. From 01.02.2014 to 30.06.2014) 2. Asmita Dash, M.Sc. Biotech student, Sambalpur University (Influence of Xmnl polymorphism

on the clinical severity of Sickle Cell Disease. From 01.02.2014 to 30.06.2014) Batch-II (6 months): 3. Aman Rathod, M.Sc. Biotech student, Sambalpur University (Characterization of

hemoglobinopathies using CE-HPLC and their hematological and biochemical profile. From 04.02.2016 to 30.06.2016)

4. Rasmita Mohanty, M.Sc. Biotech student, Sambalpur University (Reticulocyte count as a hematopoietic stress marker in Sickle Cell Disease. From 19.02.2016 to 30.06.2016)

5. Kaushal Sharma, M.Sc. Biotech student, Sambalpur University (Molecular, hematological and Biochemical profile of Sickle Cell Anaemia and Sickle-β-Thalassemia [IVSI-5 (G→C)] .From 25.02.2016 to 30.06.2016)

Batch-III: (Summer Internship: 10 days) 1. Miss. Sneha George, roll no: 1560110, 1st year B.Tech/M.Tech (Dual degree) in Biotechnology,

KIIT University, Bhubaneswar, Odisha, India, from 20.06.2016 – 30.06.2016 as an observer. 2. Miss. Shivangi Srivastava, roll no: 1560100, 1st year B.Tech/M.Tech (Dual degree) in

Biotechnology, KIIT University, Bhubaneswar, Odisha, India, from 20.06.2016 – 30.06.2016 as an observer.

Batch-IV: (6 months) 1. Miss. Kirtemayee Pradhan, roll No. 15BT08, 2nd year M.Sc. (Biotech) Sambalpur University

(Use of PCR technology in diagnosis of Sickle Cell Disease) from 01.02.2017 to 30.06.2017.

2. Miss. Mamata Ranjit, roll No. 15BT10, 2nd year M.Sc. (Biotech) Sambalpur University (DNA banking in Sickle Cell Disease patients of Sickle Cell Institute, VIMSAR Burla, Odisha) from 01.02.2017 to 30.06.2017.

ANNEXURE 3

9. Publications 2017 1. Pradeep Kumar Mohanty, Satyabrata Meher, Snehadhini Dehury, Subhra Bhattacharya, Kishalaya Das,

Siris Patel, Biswanath Sarkar. Compound heterozygote of HbDIran [HBB: c.67 G>C, 22 (b4) Glu>Gln] with

0 Thalassaemia [cds. 41/42 (-CTTT)] from eastern India. (Communicated)

2016 2. Satyabrata Meher, Snehadhini Dehury, Arpita Jana, Subhra Bhattacharya, Amrita Patel, Kishalaya Das,

Siris Patel, Biswanath Sarkar, Pradeep Kumar Mohanty. First report of Hb Limassol [b8 (A5) Lys-Asn; HBB: c.27G>C] from Odisha, India. Hemoglobin, 2016; 40(6): 422-424.

3. Satyabrata Meher, Snehadhini Dehury, Pradeep Kumar Mohanty, Siris Patel, Chinmayee Pattanayak, Subhra Bhattacharya, Kishalaya Das, Biswanath Sarkar. Hb Tianshui (HBB: c.119A>G) in Compound Heterozygosity with Hb S (HBB: c.20A>T) from Odisha, India. 2016. Hemoglobin, 40(4):270-272.

4. Purohit P, Mohanty PK, Patel S, Dehury S, Meher S, Das K, Rath A, Hati J, Behera AK. Clinical Spectrum of Severe Plasmodium falciparum Malaria in a Tertiary Care Centre of Eastern India. International Journal of Tropical Disease & Health. 2016;12(4): 1-11.

2015 5. Patel D.K., Mashon R.S, Purohit P, Meher S., Dehury S., Marndi C., Das K., Kullu B.K., Patel S., Das P.

Influence of sickle cell gene on the allelic diversity at the msp-1 locus of Plasmodium falciparum in adult patients with severe malaria. Mediterr J Hematol Infect Dis 201 5, 7(1): e2015000, DOI: http://dx.doi.org/ 10.4084/MJHID.2015.000

6. Dehury S, Purohit P, Meher S, Das K, Patel S. Compound heterozygous state of β-thalassemia with IVS1-5 (G→C) mutation and Indian deletion-inversion Gγ(Aγδβ)0-thalassemia in eastern India. Rev Bras Hematol Hemoter. 2015.

7. Maske MM, Meher S, Dora P, Dehury S, Kullu BK, Kumar Patel DK, Mahapatra S, Mohanty PK, Das K, Patel S. Nephrotic syndrome in Sickle Cell Disease of Western Odisha, India: A case report of five cases. International Journal of Pharmaceutical Science Invention. Vol 4(7). 2015. 28-31.

8. Ullatil V, Patel DK, Patel S, Das K, Bag S, Meher S. Hepatitis-B and C in Sickle Cell Hemoglobinopathies of Western Odisha, India. International Journal of Pharmaceutical Science Invention. Vol 4(5). 2015. 21-2.

9. Patel S, Dehury S, Purohit P, Meher S, Das K. Inheritance of Hereditary Persistence of Fetal Haemoglobin (HPFH) in a Family of Western Odisha, India. Journal of Clinical and Diagnostic Research. 2015 Sep, Vol-9(9). 9-10.

10. Dehury S, Purohit P, Patel S, Meher S, Kullu BK, Sahoo LK, Patel NK, Mohapatra AK, Das K, Patel DK. Low and Fixed Dose of Hydroxyurea is Effective and Safe in Patients With HbSβ+ Thalassemia With IVS1-5(G→C) Mutation. Pediatr Blood Cancer 2015: 62(6).1017-1023.

2014 11. Dehury S, Purohit P, Patel S, Meher S, Kullu BK, Sahoo LK, Patel NK, Mohapatra AK, Das K, Patel DK. Low

and fixed dose of hydroxyurea is effective and safe in patients with HbSbeta thalassemia with IVS1-5(G-->C) mutation. Pediatr Blood Cancer 2014.

12. Patel S, Purohit P, Mashon RS, Dehury S, Meher S, Sahoo S, Dash SS, Das K, Das P, Patel DK. The effect of hydroxyurea on compound heterozygotes for sickle cell-hemoglobin D-Punjab-A single centre experience in eastern India. Pediatr Blood Cancer 2014:61:1341–1346.

13. Purohit P, Dehury S, Patel S, Patel DK. Prevalence of deletional alpha thalassemia and sickle gene in a tribal dominated malaria endemic area of eastern India. ISRN Hematology. 2014 (2014), ID 745245, http://dx.doi.org/10.1155/2014/745245.

14. Patel DK, Mohapatra MK, Thomas AG, Patel S, Purohit P. Procalcitonin as a biomarker of Bacterial infection in sickle cell vaso-occlusive crisis. Mediterranean Journal of Hematology and Infectious Diseases. 2014; 6: e2014018.

15. Purohit P, Mashon RS, Patel S, Dehury S, Pattanayak C, Das K, Nair S, Italia K, Bag S, Colah RB, Patel DK. Clinical and molecular characterization of Hb Hofu in eastern India. International Journal of Laboratory Hematology. 2014;36:71-76.

16. Patel S, Meher S, Dehury S, Purohit P, Das K, Patel DK, Mohanty PK. Molecular, Hematological and clinical characterization of sickle cell HbE in Eastern India: The Largest Series in world. Ind J Haemat. Blood Transf. 2014; 30(2): S448-S546. (Abstract)

17. Purohit P, Patel DK, Patel S, Mohanty PK, Dehury S, Meher S, Das K. Alpha-thalassemia protects against severity of P. falciparum malaria in eastern India-A hospital based study. Ind J Haemat. Blood Transf. 2014: 30(2): S448-S546. (Abstract)

18. Meher S, Patel DK, Patel S, Das K, Dehury S, Purohit P, Jana A, Bhattacharya S, Mohanty PK, Sarkar B. HbTianshui (β39; CAG>CGG, Gln>Arg): First report of a rare Hemoglobin variant from India. Ind J Haemat. Blood Transf. 2014: 30(2): S448-S546. (Abstract)

19. Dehury S, Sarkar B, Patel DK, Meher S, Purohit P, Jana A, Bhattacharya S, Das K, Mohanty PK, Patel S. A rare Domonant beta thalassemia mutation in association with HbS: First report of HbWestdale (cd126_131:17 bp deletion) from Odisha, India. Ind J Haemat. Blood Transf. 2014; 30(2): S448-S546. (Abstract)

20. Dehury S, Sarkar B, Patel DK, Meher S, Purohit P, Jana A, Bhattacharya S, Das K, Mohanty PK, Patel S. A rare Domonant beta thalassemia mutation in association with HbS: First report of HbWestdale (cd126_131:17 bp deletion) from Odisha, India. Ind J Haemat. Blood Transf. 2014: 30(2): S448-S546. (Abstract).

2013 21. Patel DK, Purohit P, Dehury S, Das, Dutta A, Meher S, Patel S, Bag S, Mashon R. S, Das K. Fetal

hemoglobin and alpha thalassemia modulate the phenotypic expression of HbSD-Punjab. International Journal of Laboratory Hematology.2013:1-7.

2012 22. Meher S, Patel DK, Patel S, Dehury S, Purohit P, Das K. Epidemiology of Beta Thalassaemia Trait in Eastern

India. Orissa Physicians Journal, 2012: 9; 24-29. 23. Patel DK, Mashon RS, Patel S, Das BS, Purohit P, Biswal S. Low dose hydroxyurea is effective in reducing

the incidence of painful crisis and frequency of blood transfusion in sickle cell anemia patients from eastern India. Hemoglobin. 2012; 36(5):409-420.

24. Purohit P, Patel DK, Patel S, Dehury S, Bishwal SC, Meher S, Padhan B, Das K. Trial of Hydroxyurea in sickle cell haemoglobinopathies patients of eastern India, Ind J Haemat. Blood Transf. 2012; 28(4): 223. (Abstract)

25. Bishwal SC, Patel DK, Patel S, Dehury S, Purohit P, Meher S, Pradhan B, Das K. Clinical, Hematological and Molecular characterization of sickle cell HbDPunjab (HbSD) in eastern India: The largest series in world. Ind J Haemat. Blood Transf. 2012; 28(4):223. (Abstract)

26. Meher S, Patel DK, Patel S, Purohit P, Dehury S, Bishwal SC, Pradhan B, Das K. Epidemiology of beta thalassemia trait in western Odisha. Ind J Haemat. Blood Transf. 2012;28(4):224. (Abstract)

27. Dehury S, Patel DK, Patel S, Purohit P, Bishwal SC, Meher S, Pradhan B, Das K. Clinical and Molecular characterization of 194 cases of Sickle-beta thalassemia in western Odisha and their response to Hydroxyurea therapy. Ind J Haemat. Blood Transf. 2012; 28(4):224. (Abstract)

28. Patel S, Patel DK, Purohit P, Dehury S, Bishwal SC, Meher S, Pradhan B, Das K. The “Odisha Sickle Cell Project”- a new horizon of hope for sickle cell aggrieved patients in Odisha. Ind J Haemat. Blood Transf. 2012; 28(4):224. (Abstract)

2011 29. Basu S, Dash BP, Patel DK, Chakravarty S, Chakravarty A, Banerjee D, Chakrabarti A. F-cell levels are

altered with erythrocyte density in sickle cell disease. Blood Cells, Molecules, and Diseases, 2011; 47: 117–119.

30. Patel DK, Patel S, Mashon RS, Dash PM, Mukherjee MB. Diverse phenotypic expression of sickle cell haemoglobin C disease in an Indian family. Ann Hematol. 2011;90(3):357-358.

31. Patel DK, Purohit P, Jena SK, Patel S & Padhi PK, Clinical profile of leptospirosis in a tertiary care medical center of western Orissa, Orissa physician journals, 2011;7: 13-17.

2010 32. Patel DK, Mashon RS, Patel S, Dash PM, Das BS. β globin gene Haplotypes linked with the HbDPunjab

(β121(GH4)Glu-Gln, GAA>CAA) mutation in eastern India. Hemoglobin, 2010;34(6):530-537. 33. Patel DK, Patel M, Mashon RS, Patel S, Dash PM, Das BS. Clinical and Molecular characterisation of βS &

Gg(Agdb)0-Thalassemia in eastern India. Hemoglobin, 2010; 34(6): 604-609. 2009 34. Mashon RS, Dash PM, Khalko J, Dash L, Mohanty PK, Patel S, Patel RC, Dash BS, Dash UK, Das PK, Patel

PK. Higher fetal hemoglobin concentration in patients with sickle cell disease in eastern India reduces frequency of painful crisis. European Journal of Hematology, 2009; 83(4):383-384.

35. Dash P, Patel DK, Patel S, Mashon RS. Molecular characterization of α α/ - α 3.7 deletion on hematological and clinical heterogeneity of sickle- beta0 thalassemia in western Orissa, India. Ind J Haemat. Blood Transf., 2009, Vol 25, p140. (Abstract)

2008 36. Patel DK, Patel S, Mashon RS, Dash P, Prenatal diagnosis of inherited hemoglobin disorders. Journal of

Community Medicine, Vol. 4, No. 1, Jan 2008, Pp 20-25. 37. Patel DK, Sethy RS, Mohanty PK et al. Chronic myeloid leukemia presenting as extramedullary disease in

form of pseudochloroma of the ovary: a rare case report .The ind.Pract.2008;61(2):104-106. 38. Patel DK, Dash LK,Mohanty PK et al. Bilateral Pedal edema following sideflash lightening injury mimicking

Keraunoparalysis. Jounral of Community Medicine.2008;4(2):45-47 2007 39. Mohanty PK, Patel DK. Observation on Acute accidental inhalational exposure to chlorine gas in adults

.Orissa physicians jounaral.2007;3:7-9. 40. Patel DK, Mashon RS, Padhy PK, Patel S, Mohanty PK et al. Sickle disease- Review and experience in VSS

Medical College, Burla. Orissa Physicians Jounral. 2007;3:26-36. 41. Patel DK, Dash P, Mashon RS, Mohanty PK et al. Molecular diagnostic techniques in hemaglobinapathies

and infectious disease: Current scenario in Western Orissa. Jounral of Community Medicine.2007;3(2):51-58.

42. Patel DK,Mohanty PK,Padhy PK,Mashon RS et el. Eliss-van Creveld syndrome with sickle cell hemoglobinopathy and dyserythropoiesis: a rare combination Jounaral of community Medicine.2007;3(1):41-43.

2006 43. Patel DK, Mohanty PK, Padhi PK, Dash AK; Clinical Profile of Tropical pyomyosities in a Tertiary care

Hospital in western Orissa., Journal of Community Medicine, 2006, Vol. 2; No-2; P 82-84. 44. Padhi PK Patel DK, Mohanty PK, Sahoo SC, Khan S; Factors predicting morbidity & mortality in Idiopathic

Dilated Cardiomyopathy - An in hospital survey. JAPI, 2006; vol-54; P 587-88. 45. Mohanty S, Patel DK, Pati SS, Mishra SK, Adjuvant therapy in cerebral malaria Indian Journal of Medical

Research 2006, 124 (3), 245-60. 46. Mohanty BN, Das BK, Kuanr BK, Mohanty RM, Sarangi CR, Mohanty AC, Patel DK, Mangual R, Panigrahi D,

Mishra SK, Analysing four years of experience in use of Telemedicine in a state level tertiary care medical institution. Abstract book of Global Convention & Expo on Telemedicine & e-Health – 2006. p- 60.

47. Mishra R, Agarwal A, Agarwal V, Lawrence A, Das B, Patel DK, Basnet R, Kapoor L, Mishra SK. Telemedicine as a tool of rheumatology teaching of Post graduate level., Abstract book of Global Convention & Expo on Telemedicine & e-Health – 2006. p.69.

2005 48. Patel DK, Mohanty PK, Pattnaik SR, Padhi PK, Guhathakurta S. Study of glycemic status & pancreatic

pathology in sickle cell disease patients in Western Orissa: Jr. Asso. Phy. India (JAPI). Vol.53, Apr-2005-p.367-368.

2003 49. Patel DK, Patel S, Mohanty PK, Majhi CD, Nanda R. Observation of various clinical status and

complications in sickle cell trait in. Western Orissa: Jr. Asso. Phy. India (JAPI) 2003 Vol. 51 -p-1243. 2002 50. Patel DK, Panda A., Panda R.S. Study of cardiovascular dysfunction in sickle cell hemoglobinopathy in

western Orissa.: Jr. Asso. Phy. India (JAPI), Vol. 50, Jan2002. p.756-57. 2001 51. Patel DK, Panda NC, Panda RS. Study of Vaso-occlusive crisis in sickle cell trait: Jr. Asso. Phy. India (JAPI),

Vol 49, Jan 2001. p. 97. 2000 52. Priapism is rare in sickle cell disease in India. Dash BP, Kar BC. J Ass Phys Ind; 48: 255-256, 2000. 1999 53. Splenectomy in sickle cell disease in Orissa. Kar BC.; Ass Phys Ind, 47: 890. 1999. 54. Osmotic fragility of Normal and sickle hemoglobin containing red blood cells. Dash BP, Mitra A, Kar BC.;

Ind J Physiol and Pharmaco, 43 (II): 267-269, 1999. 1997 55. Panda RS., Patel DK, Rath S. C. Study of Immunological Markers of Hepatitis B Viral Infection in Patients

of Sickle Cell Disease in a Rural Medical College of Orissa: The Indian Practitioner, July, 1997, Vo1.50, No.7, p. 583-586.

56. Cholelythiasis in sickle cell disease in India. Tripathy D, Dash BP, Mohapatra BN, Kar BC,. Ass Phys Ind. 45: 287, 1997.

57. Clinical profile of sickle cell disease in Orissa. Kar BC. Indian J. Pediatrics, 64: 73, 1997. 1996 58. Dash BP, Kar BC. Hemoglobin SD Disease in Muslim boy. Ind J Hematol Blood Transf., 14: 159-160, 1996.

59. Possible gene link of high HbF in I ndian Sickle cell Disease (SS) patients. Dash BP, Kar BC,: Ind J Hematol Blood Transf., 13: 165, 1995.

1995 60. Patel DK, Panda RS. Paul P, Plasma Viscosity in Sickle Cell Hemoglobinopathy.: The Indian Practitioner,

Jan. 1995, Vol. XLVIII, No.1, p. 25-29. 1994 61. Kar BC, Devi S, Pattanaik G, Dash BP, Das RK. HbF Levels do not determine the clinical course of SS

disease. : Ind J Hematol Blood Transf., 12: 225, 1994. 1993 62. Dash BP, Das RK, Pattanaik G, Kar BC. Foetal Hemoglobin in normal, Sickle cell Disease patients in

western Orissa, India. Ind J Hematol Blood Transf 11:232-236, 1993. 63. Mohapatra BN, Dash BP., Kar BC. Serum Immunoglobin in Sickle cell disease.; J Ass Phys., Ind 41: 418-

419, 1993. 1992 64. Pani S, Dash BP, Kar BC. G6PD Deficiency and sickle cell Disease in India.; Ind J Hematol Blood Transf,

10:85-88,1992. 65. Dash BP, Mitra A , Kar BC. A study on the glucose uptake, pyruvate and lactate formation in red blood

cells of normal sickle cell trait and sickle cell patients. Ind J Clin Biochem 7 (II): 134-137, 1992. 1991 66. Kar BC. Sickle cell disease in India (1991) Journal of the Association of Physicians of India 39(12): 954–

960. 67. Kulozik AE, Bail S, Kar B.C., Serjeant BE, Serjeant G.E. Sickle Cell β+ Thalassemia in Orissa state, India; Brit.

J Hematol, 77:215, 1991. 1990 68. Kar BC, Agrawal KC, Panda A. Sickle Hemoglobin, G6PD deficiency and Malaria in Western Orissa; J Ass.

Phys. Ind, 389, 555-557, 1990. 1988 69. Molecular basis of alpha thalassaemia in India. Its interaction with the Sickle Cell Gene. Blood 71: 467,

1988. 1987 70. Kar BC, Devi S, Dash KC, Das M. The Sickle Cell Gene is widespread in India. Trns. Royal Soc. Trop. Med.

Hygiene, 81: 273,1987. 71. Kulozik AE, Kar B.C, Satpathy R.K et al. Fetal Hemoglobin level and βs Globin gene haplotypes in an Indian

population with Sickle Cell diabetes; Blood, 89:1742. 1987. 1986 72. Kar, B.C. and Satpathy R.K et al. Sickle Cell Disease in Orissa State, India. Lancet II: 1199, 1986. 73. Geographical survey of βs Globin gene haplotype, evidence for an independent Asian Origin. Kulozik AE,

Winscoat J.S., Serjeant G.R., Kar B.C.; et al Am J Hum Genet 39:239, 1986.

ANNEXURE 4 13. PG Dissertation for M.D. (General Medicine) degree done on Sickel Cell Hemoglobinopathy

Post Graduate Thesis submitted to VIMSAR, Burla (formerly VSS Medical College, Burla Odisha). 1. Role of fetal hemoglobin to the different complications of sickle cell disease in India. Dr. N. Dora. 2. Level of serum Hemocystine level in Type – 2 DM. Dr. A. K. Behera. 3. Study of sickle cell trait & its manifestation with special reference to vasso-occlusive crisis. Dr.

Rajesh Nanda. 4. Sero-prevalence of hepatitis B virus and hepatitis C virus infection in sickle cell

Haemoglobinopathy. Dr. Prasanta Pradhan. 5. Study of impaired glucose tolerance, diabetes mellitus and pancreatic pathology in sickle cell

Haemoglobinopathy. Dr. Sudeshna Guhathakurta. 6. Evaluation of Iron status in Sickle Cell hemoglobinopathy in Western Orissa. Dr. Biswaranjan

Jena. 7. Moniotoring the acute phase response with estimation of C-reactive protein in Sickle Cell Disease

with vasso occlusive crisis. Dr. S. Singh. 8. Study on prevalence of G6PD deficiency in Plasmodium falciparum malaria patient and correlate

with morbidity & mortality. Dr. D. D. Biswal. 9. Trial of Hydroxyurea in treatment of Sickle Cell Disease in a tertiary care Hospital in Western

Orissa. Dr. Lalit Pradhan. 10. PCR diagnosis & genotyping of Plasmodium infection in a tertiary care medical centre in Western

Orissa. Dr. Sanjeev. 11. Influence of ABO blood group, homozygous & heterozygous alpha thalassemia on the severity of

falciparum malaria in India. Dr. Jagannath Hati (2011). 12. Correlation of LDH as a biomarker of hemolysis and various sickle phenotypes like leg ulceration,

priapism, vaso-occlusive crisis and pulmonary hypertension. Dr Chitralekha Naik. (2012). 13. Influence of Sickle cell haemoglobinapathies on the severity of P. falciparum malaria in Western

Odisha. Dr. P. Shyam Sundar (2012). 14. Study of procalcitonin in sickle cell disease patients in Western Odisha. Dr AG Thomas (2013). 15. Effect of Hydroxyurea on Male Gonadal Function in Sickle Cell Anemia Patients. Dr. Lulup Kumar

Sahu (2013). 16. Hepatitis-B and C in Sickle Cell Hemoglobinopathies of Western Odisha, India- Dr Vinod Ullatil

(2014). 17. Homocysteine levels in sickle cell disease- Dr. Mahendra M Maske (2014). 18. Prevalence and risk factors for pulmonary hypertension in Sickle Cell Disease patients of eastern

India- Dr Debashis Maikap (2015). 19. Response of Hydroxyurea Therapy in Sickle Cell-D Punjab disease- Dr. Santosh Kumar Swain

(2016).

C. M.Phil Thesis awarded on Sickle Cell submitted to Sambalpur University, Odisha

1. Molecular Epidemiology and clinical aspect of sickle cell syndrome of Bargarh District, Odisha, India. Awarded to: Mr. Satyabrata Meher (2012).

D. Ph. D Thesis awarded on Sickle Cell submitted to Sambalpur University, Odisha

1. Study of Genetic Diversity of Sickle Cell Disease and its Phenotypic Expressions in Association with other Inherited Globin Gene Disorders in Western Orissa, India. Awarded to: Dr Preetinanda Manaswini Dash (2011).

2. Beta globin gene cluster haplotype study of Indian population and its correlation with hematological parameters and clinical severity of Sickle Cell Disease. Awarded to: Dr Ranjeet Singh Mashon (2011).

3. Influence of host genetic polymorphisms on the severity of Plasmodium falciparum malaria with special reference to Sickle cell gene and Alpha thalassaemia. Ongoing Ph.D work by Mr Prasanta Purohit.

ANNEXURE 5 14.1. & 14.2: Paper presentation & Conference participation 2017

1. Dr Kishalaya Das. Participation in the 14th Thalassemia user meet & 7th Thalassemia & HbA1c user

meet-Hemoglobin Update Meet" on 8th of July ,Saturday at Hotel Taj Bengal, Kolkata.

2. Pradeep Kumar Mohanty. Activities in Odisha Sickle Cell Project at VIMSAR, Burla. In 3rd Global

Congress on Sickle Cell Disease held at Bhubaneswar, Odisha, India during 21st to 24th of February

2017. Oral Presentation.

3. Siris Patel. Experience with Hydroxyurea in Odisha Sickle Cell Project, VIMSAR, Burla. In 3rd Global

Congress on Sickle Cell Disease held at Bhubaneswar, Odisha, India during 21st to 24th of February

2017. Oral Presentation.

4. Siris Patel. Reproductive outcome of women with Sickle Cell Disease: A retrospective study from

western Odisha, India. In 3rd Global Congress on Sickle Cell Disease held at Bhubaneswar, Odisha,

India during 21st to 24th of February 2017. Oral Presentation.

5. Kishalaya Das. Spectrum of abnormal haemoglobinopathies and sickle cell disease in Odisha: six years

experience of the Odisha Sickle Cell Project (NHM Odisha), VIMSAR, Burla. In 3rd Global Congress on

Sickle Cell Disease held at Bhubaneswar, Odisha, India during 21st to 24th of February 2017. Oral

Presentation.

6. Satyabrata Meher, Pradeep Kumar Mohanty, Siris Patel, Kishalaya Das, Snehadhini Dehury, Sarmila

Sahoo, Punam Dora, Manaranjan Das, Bisnu Prasad Dash. Genotypes and serum Haptoglobin level in

mild and severe Sickle Cell Anemia patients of Odisha. In 3rd Global Congress on Sickle Cell Disease

held at Bhubaneswar, Odisha, India during 21st to 24th of February 2017. Oral Presentation.

7. Satyabrata Meher, Pradeep Kumar Mohanty, Siris Patel, Kishalaya Das, Snehadhini Dehury, Sarmila

Sahoo, Punam Dora, Manaranjan Das. Reticulocyte Count as a hematopoietic stress marker in Sickle

Cell Disease. In 3rd Global Congress on Sickle Cell Disease held at Bhubaneswar, Odisha, India during

21st to 24th of February 2017. ePoster Presentation.

8. Snehadhini Dehury, Pradeep Kumar Mohanty, Siris Patel, Satyabrata Meher, Sarmila Sahoo,Kishalaya

Das,Jagnyeswar Ratha. Clinical and molecular characterization of 472 cases with Sickle--

thalassaemia in Western Odisha. In 3rd Global Congress on Sickle Cell Disease held at Bhubaneswar,

Odisha, India during 21st to 24th of February 2017. ePoster Presentation.

9. Sarmila Sahoo, Pradeep Kumar Mohanty, Siris Patel, Snehadhini Dehury, Satyabrata Meher, Kishalaya

Das. Exchange HPLC as a novel tool for detection of different genotypes of sickle cell disease. In 3rd

Global Congress on Sickle Cell Disease held at Bhubaneswar, Odisha, India during 21st to 24th of

February 2017. ePoster Presentation

2016

10. Satyabrata Meher, Pradeep Kumar Mohanty, Siris Patel, Snehadhini Dehury, Kishalaya Das, Sarmila

Sahoo. “Rare hemoglobin variants from Odisha, India”. at Chandigarh on “Thalassemia and Sickle Cell

Disease” Bilateral Workshop on 5th – 6th November 2016. Poster Presentation.

11. Siris Patel, Pradeep Kumar Mohanty, Satyabrata Meher, Snehadhini Dehury, Prasanta Purohit,

Sarmila Sahoo, Kishalaya Das, Punam Dora, Manoranjan Das. “Screening and Management of

patients with sickle cell disease: An experience of Odisha Sickle Cell Project” at Chandigarh on

“Thalassemia and Sickle Cell Disease” Bilateral Workshop on 5th – 6th November 2016. Poster

Presentation.

12. Snehadhini Dehury, Pradeep Kumar Mohanty,Siris Patel,Prasanta Purohit,Satyabrata Meher,Kishalaya

Das. Influence of alpha thalassemia on hematological and clinical profile of patients with Sβ+

thalassemia. at Chandigarh on “Thalassemia and Sickle Cell Disease” Bilateral Workshop on 5th – 6th

November 2016. Poster Presentation.

2015

13. Satyabrata Meher, Snehadhini Dehury, Kishalaya Das, Pradeep Kumar Mohanty, Prasanta Purohit,

Dilip Kumar Patel, Biswanath Sarkar, Roshan Colah,Siris Patel. “Sickle Cell Disease in Western Odisha”

at National seminar on sickle cell anaemia 2015, Pune, Maharashtra (India) on 26th march 2015.

Poster Presentation

14. Satyabrata Meher, Mahendra M Maske, Snehadhini Dehury, Prasanta Purohit, Pradeep Kumar

Mohanty, Kishalaya Das, Siris Patel “Plasma homocystein level a marker of vaso-occlusive crisis in

Sickle Cell Disease.” 56th Annual meeting of the Indian Society of Haematology and Blood

Transfusion (ISHBT) held at The Lalit Ashok, Bangalore, from 5th -8th September 2015. Poster

Presentation

15. Siris Patel, Satyabrata Meher, Snehadhini Dehury, Kishalaya Das, Pradeep Kumar Mohanty, Prasanta

Purohit, Dilip Kumar Patel. Odisha Sickle Cell Project – The hope for Sickle Cell Disease patient in

Odisha. at National seminar on sickle cell anaemia 2015, Pune, Maharashtra (India) on 26th march

2015. Poster Presentation

16. Satyabrata Meher, Sushil Kumar Sahu, Snehadhini Dehury, Kishalaya Das, Pradeep Kumar Mohanty,

Dilip Kumar Patel, Siris Patel. Association between XmnI Polymorphism (rs7482144) and HbF in Sickle

Cell Disease: Evidence from a Meta-Analysis. Indian Genetic Congress at SRM university Chennai. IGC-

2015.Oral Presentation

17. Prasanta Purohit, Siris Patel, Snehadhini Dehury, Satyabrata Meher, Kishalaya Das, Sonamali Bag,

Dilip Kumar Patel, Pradeep Kumar Mohanty. Genetic determinants and response to hydroxyurea

therapy in patients with sickle cell anemia. Indian Genetic Congress at SRM university Chennai. IGC-

2015. Poster Presentation

18. Satyabrata Meher, Dilip Kumar Patel, Siris Patel, Kishalaya Das, Snehadhini Dehury, Prasanta Purohit,

Arpita Jana, Subhra Bhattacharya, Pradeep Kumar Mohanty, Biswanath Sarkar . A novel β0-

Thalassaemia mutation Cd-15(-T) in Compound Heterozygote first time with Sickle Cell gene from

Eastern India. IJHG. 2015. Poster Presentation

19. Siris Patel, Satyabrata Meher, Dr Pradeep K Mohanty, Snehadhini Dehury, Prasanta Purohit, Dr

Poonam Dora, Dr K Das, Dr Dillip Kumar Patel. Clinical, Hematological, Molecular Characterization

and response to Hydroxyurea treatment of symptomatic 17 HbSE cases in Eastern India:The largest

series in world. IJHG. 2015. Poster Presentation

20. Snehadhini Dehury, Siris Patel, Prasanta Purohit, Satyabrata Meher, Kishalaya Das, Dilip Kumar Patel,

Pradeep Kumar Mohanty. Genotype-phenotype correlation in patients with HbS-thalassaemia in

eastern India. IJHG. 2015. Poster Presentation

21. Prasanta Purohit, Siris Patel, Snehadhini Dehury, Satyabrata Meher, Kishalaya Das Dilip Kumar Patel,

Pradeep Kumar Mohanty. Negative epistatic interaction of sickle cell trait and alpha-thalassemia

against severe P. falciparum malaria. IJHG. 2015. Poster Presentation

2014

22. Meher S, Patel DK, Patel S, Das K, Dehury S, Purohit P, Jana A, Bhattacharya S, Mohanty PK, Sarkar B.

HbTianshui (β39; CAG>CGG, Gln>Arg): First report of a rare Hemoglobin variant from India. Ind J

Haemat. Blood Transf. 2014;30(2): S448-S546. Poster Presentation

23. Patel S, Meher S, Dehury S, Purohit P, Das K, Patel DK, Mohanty PK. Molecular, Hematological and

clinical characterization of sickle cell HbE in Eastern India: The Largest Series in world. Ind J Haemat.

Blood Transf. 2014;30(2): S448-S546. Poster Presentation

24. Purohit P, Patel DK, Patel S, Mohanty PK, Dehury S, Meher S, Das K. Alpha-thalassemia protects

against severity of P. falciparum malaria in eastern India-A hospital based study. Ind J Haemat. Blood

Transf. 2014;30(2): S448-S546. Poster Presentation

25. Dehury S, Sarkar B, Patel DK, Meher S, Purohit P, Jana A, Bhattacharya S, Das K, Mohanty PK, Patel

S. A rare Domonant beta thalassemia mutation in association with HbS: First report of HbWestdale

(cd126_131:17 bp deletion) from Odisha, India. Ind J Haemat. Blood Transf. 2014;30(2): S448-S546.

Poster Presentation

26. Meher S, Patel DK, Patel S, Dehury S, Purohit P, Das K. “Patients with sickle cell -β0 thalassemia (cd

15(-T) mutation: First case in India” in International CME on hematology and 6th Annual meet of

Odisha hematology, Cuttack, Odisha. 2014. Oral Presentation

27. Dehury S, Patel DK, Patel S, Purohit P, Meher S, Das K. “First report on double heterozygous state of

HbS/HbWestdale (cd.126_131:17 bp deletion) from Odisha”. in International CME on hematology

and 6th Annual meet of Odisha hematology, Cuttack, Odisha.2014

28. Purohit P, Patel DK, Patel S, Dehury S, Meher S, Das K. “Haematological and Clinical variability of

double heterozygous state of HbSD-Punjab”. in International CME on hematology and 6th Annual

meet of Odisha hematology, Cuttack, Odisha.2014

2012

29. Meher S, Patel DK, Patel S, Purohit P, Dehury S, Bishwal SC, Pradhan B, Das K. Epidemiology of beta

thalassemia trait in western Odisha. Ind J Haemat. Blood Transf. 2012;28(4):224. Poster Presentation

30. Patel S, Patel DK, Purohit P, Dehury S, Bishwal SC, Meher S, Pradhan B, Das K. The “Odisha Sickle Cell

Project”- a new horizon of hope for sickle cell aggrieved patients in Odisha. Ind J Haemat. Blood

Transf. 2012;28(4):224. Poster Presentation

31. Dehury S, Patel DK, Patel S, Purohit P, Bishwal SC, Meher S, Pradhan B, Das K. Clinical and Molecular

characterization of 194 cases of Sickle-beta thalassemia in western Odisha and their response to

Hydroxyurea therapy. Ind J Haemat. Blood Transf. 2012;28(4):224. Poster Presentation

32. Purohit P, Patel DK, Patel S, Dehury S, Bishwal SC, Meher S, Pradhan B, Das K. Trial of Hydroxyurea in

sickle cell haemoglobinopathies patients of eastern India, Ind J Haemat. Blood Transf. 2012; 28(4):

223. Poster Presentation

33. Bishwal SC, Patel DK, Patel S, Dehury S, Purohit P, Meher S, Pradhan B, Das K. Clinical, Hematological

and Molecular characterization of sickle cell HbDPunjab (HbSD) in eastern India: The largest series in

world. Ind J Haemat. Blood Transf. 2012;28(4):223. Poster Presentation

34. Purohit P, Patel DK, Patel S, Bishwal S, Dehury, Meher S. Prevalence and influence of alpha

thalassemia (-3.7 kb deletion) in Sickle Cell Disease patients of Western Odisha in International

conference on Molecular medicine and gene, genomics and chronic disease (MOLMED-2012). Center

of Bio-separation Technology, VIT University. 19th to 22nd February-2012. Pg. 162-163. Poster

Presentation

35. Purohit P, Patel DK, Patel S, Das K, Basu S, Jain P. Flow cytometric analysis of distribution of HbF in

erythrocytes in sickle cell disease, in 5th Annual meeting of the cytometry Society, India & 13th INDO-

US workshop on Advanced Flow Cytometry Techniques. CRNN, University of Calcutta & IPGMER,

Govt. of West Bengal, 12th to 17th October, 2012. pp-10.

36. Purohit P, Patel DK, Patel S, Bishwal SC, Dehury S. Dash PM. Influence of Alpha Thalassemia (-α/αα) in

Sickle Cell Disease & Sβ-Thalassemia patients of Western Odisha. in International Conference on

Genes, Genetics & Genomics: Today & tomorrow- Human concerns and 37th Annual conference of

The Indian Society of Human Genetics. Punjab University, Chandigarh, India. March-2012:pp-145.

37. Dehury S, Patel DK, Patel S, Bishwal SC, Purohit P. Molecular diagnosis of high HbF determinant in

population of Odisha in Eastern India, in International Conference on Genes, Genetics & Genomics:

Today & tomorrow- Human concerns and 37th Annual conference of The Indian Society of Human

Genetics. Punjab University, Chandigarh, India. March-2012: pp-146.

38. Bishwal SC, Patel DK, Patel S, Dehury S, Purohit P. Clinico-Hematological profile of HbSS and HbSD

patients in a tertiary care Sickle Cell Centre of Western India, in International Conference on Genes,

Genetics & Genomics: Today & tomorrow- Human concerns and 37th Annual conference of The

Indian Society of Human Genetics. Punjab University, Chandigarh, India. March-2012: pp-142.

2011

39. Dilip Kumar Patel, Gobinda Prasad Nayak, Management of sickle cell disease in adults: Experience at

VSS Medical College, Burla-A comprehensive sickle cell center located in eastern India. in CME ON

SICKLE CELL DISEASE & SOUVENIR, 1st Dr. B.C.Kar Memorial International Conference & CME on

sickle cell disease. V.S.S.Medical College, Burla, Sambalpur, Orissa. March 2011: pg 23-27.

40. Dilip Kumar Patel. Epidemiology and clinical aspects of sickle cell disease in India. in CME ON SICKLE

CELL DISEASE & SOUVENIR, 1st Dr. B.C.Kar Memorial International Conference & CME on sickle cell

disease. V.S.S.Medical College, Burla, Sambalpur, Orissa. March 2011: pg 28-38.

2010

41. Dilip Kumar Patel. Manegement of sickle cell disease in adults in India, in 4th International Congress

on Sickle cell disease (Management and prevention of sickle cell disease in developing society),

Raipur (Chhatishgarh), India. November 22nd to 27th 2010.

2009

42. Patel DK, Epidemiology and Clinical aspects of sickle cell disease in India, Lecture at 5th Brazilian

Symposium Sickle Cell Disease and other Hemoglobinopathies held from 3rd to 7th October 2009 at

Belo Horizonte, Brazil.

43. Mashon RS, Patel DK, Patel S, Dash P. Influence of sickle cell gene on Plasmodium falciparum MSP-1

and 2 alleles in symptomatic malaria. Ind J Haemat. Blood Transf., 2009, vol 25, p178.

2008

44. Patel DK, Patel S, Mashon RS, Dash P. Study of beta globin gene cluster haplotype in sickle cell

disease patients of western Orissa and correlation with various hematological parameters.” in

International Conference on Population and Medical Genetics, PGIMER, Chandigarh; Feb. 2008.

2007

45. Patel DK, Patel S , Mashon RS, Dash P, Study of beta globin gene cluster haplotype and XmnI

polymorphism in Sickle Cell Disease patients of Western Orissa (India) – A preliminary Report.”

Abstract, International Conference on Emerging trends in Haematology and Immuno Hematology,

IIH, Mumbai 31st January 2007.

2000

46. Patel DK, Patel S., Kar MK. Clinical Profile of Plasmodium Falciparum Malaria in a Geographical area of

Western Orissa associated with high incidence of sickle cell disease: Proceeding of 87th Session of

Indian Science Congress, Pune. 2000. p.36-37.

1996

47. Panda RS, Patel DK. Sickle Cell Disease with fever An Observation. Paper Presented at 9th Annual

Conference of API, Guwahati: 1996.

ANNEXURE 6 14.6. Extracurricular achievements

Sickle Cell Unit acts as a nodal center for providing service to patients and conduct research in Sickle cell hemoglobinopathy.

Total sample screened: 64279

Total SCD patients registered: 15036

Total SCT registered: 17822

Low dose Hydroxyurea therapy for SCD patients with indications:

Total SCD patients under treatment management with Hydroxyurea therapy at our centre: 8324

Sickle cell unit caters patients coming from all over Odisha and adjacent states of Jharkhand, West Bengal, Chhatishgarh, Andhara Pradesh & Telangana in addition to those of Project district free of cost.

The unit facilitates various research projects/dissertations of MSc students from Universities/ PG students of VIMSAR Burla.

The Sickle Cell project under VIMSAR, Burla has 12 viable sickle cell units at 12 western Odisha districts with higher sickle cell prevalence, which are equipped with required basic screening facilities, counselling for sickle cell traits and referral services for Sickle Cell Disease patients.

Advanced molecular research done at sickle cell institute VIMSAR Burla: The Odisha Sickle Cell Project (NHM Odisha) at Sickle Cell Institute is equipped with PCR, Flow Cytometry, DNA documentation facility, advanced molecular screening using DNA sequencing (in collaboratortion with govt of India). More than 1000 difficult DNA samples have been screened by DNA sequencing. The results in past 80 months shows 28 variable phenotypes. The findings indicate high allelic diversity of haemoglobinopathies in Odisha, many of which hitherto are not reported from India. Most common sickle cell disease genotype is HbSS. Besides this; homozygote HbS, at least nine states of compound heterozygosity of were found sickle cell haemoglobinopathy in Odisha (with S-halassaemia, HbSD, HbSE, HbSC, HbS-Hofu, HbS-Tianshui,HbS-lepore,HbS-QIndia and S-thalassemia). Nineteen non-sickling hemoglobinopathies were also found caused by 8 rare haemoglobin variants (HbD, HbE, Hb-Hofu, Hb-Limassol, Hb-lepore, Hb-QIndia, HbDIran & HbH) and thalassaemias with various allelic combinations. We have confirmed 15 cases of Indian deletion inversion G(A)0-thalassemia and HPFH-3 in several combinations. In addition, 3 rare

0-thalassaemia mutations were also confirmed in our patients. Research outcomes of progressive Odisha Sickle Cell Project (NHM Odisha), VIMSAR Burla with respect to the variation of

haemoglobinopathies in the state are remarkable. Our findings of uncommon 0-thalassaemia mutations and several rare variants strengthens the haemoglobinopathy database of the state.

Publications: Since 2010, the Odisha Sickle Cell Project (NHM Odisha) has 33 publications of research outcomes in various international and national journals of high repute. The scientific unit of the Sickle Cell Institute, VIMSAR Burla has participated in 41 international and national conferences/ seminars/ workshops.

DNA Banking: The Odisha Sickle Cell Project (NHM Odisha) at the Sickle Cell Institute, VIMSAR, Burla is equipped with state of art molecular biology laboratory facilities including DNA banking. All precious DNA samples extracted from sickle cell disease patients with variable clinical presentation and genotypes are being banked as per the guideline. The set up has ultra low freezers for safe and proper banking and retrieval equipped with full time power back up. Above 4500 DNA samples have been catalogued and banked for future utilization in purpose of further research, association studies and family studies.

ANNEXURE 7 14.7. Others

1. The Centre has been earmarked as “centre of Excellence” in the state by the hon’ble Secretary

of Health and Family Welfare, Odisha,vide Ref Letter no 6478,Dt-25.03.2015

2. Reporting of rare hemoglobin variants and rare -thalassaemia mutations

1. Satyabrata Meher, Snehadhini Dehury, Arpita Jana, Subhra Bhattacharya, Amrita Patel,

Kishalaya Das, Siris Patel, Biswanath Sarkar, Pradeep Kumar Mohanty. First report of Hb

Limassol [b8 (A5) Lys-Asn; HBB: c.27G>C] from Odisha, India. Hemoglobin, 2016; 40(6): 422-

424.

2. Satyabrata Meher, Snehadhini Dehury, Pradeep Kumar Mohanty, Siris Patel, Chinmayee

Pattanayak, Subhra Bhattacharya, Kishalaya Das, Biswanath Sarkar. Hb Tianshui (HBB:

c.119A>G) in Compound Heterozygosity with Hb S (HBB: c.20A>T) from Odisha, India. 2016.

Hemoglobin. (Accepted).

3. Purohit P, Mashon RS, Patel S, Dehury S, Pattanayak C, Das K, Nair S, Italia K, Bag S, Colah RB,

Patel DK. Clinical and molecular characterization of Hb Hofu in eastern India. International

Journal of Laboratory Hematology. 2014;36:71-76.

4. Patel DK, Patel S, Mashon RS, Dash PM, Mukherjee MB. Diverse phenotypic expression of

Sickle Cell haemoglobin C disease in an Indian family. Ann Hematol. 2011;90(3):357-358.

5. Dehury S, Sarkar B, Patel DK, Meher S, Purohit P, Jana A, Bhattacharya S, Das K, Mohanty

PK, Patel S. A rare Domonant beta thalassemia mutation in association with HbS: First report

of HbWestdale (cd126_131:17 bp deletion) from Odisha, India. Ind J Haemat. Blood Transf.

2014; 30(2): S448-S546.

6. Meher S, Patel DK, Patel S, Dehury S, Purohit P, Das K. “Patients with Sickle Cell -β0

thalassemia (cd 15(-T) mutation: First case in India” in International CME on hematology and

6th Annual meet of Odisha hematology, Cuttack, Odisha. 2014. Oral Presentation.

3. Honour to Late Dr D K Patel: Invited lecture on sickle cell disease at the 5th Brazilian

Symposium at Belo Horizonte, Brazil 2009

Patel DK, Epidemiology and Clinical aspects of sickle cell disease in India, Lecture at 5th

Brazilian Symposium Sickle Cell Disease and other Hemoglobinopathies held from 3rd to 7th

October 2009 at Belo Horizonte, Brazil.

ANNEXURE 8 15. Way Ahead: Future plans

1

Upgraded centre for mass screening by standardized laboratory methods and Councelling

Basic screening by sickling microscopy, alkaline agarose gel electrophoresis and complete blood count test facility

2 Drug distribution facility (Hydroxyurea) in all the 12 western Odisha districts with high prevalence of the disease : Free drug - Hydroxyurea capsules and folic acid tablet distribution for confirmed Sickle Cell Disease cases & comprehensive Out Patient care at all the District Sickle Cell units of the 12 western Odisha districts.

3 Prenatal Diagnosis

Looking at the alarming incidence of the sickle cell disease in the state, prevention through prenatal diagnosis using advanced molecular biology methods is necessary. Establishment of Prenatal Diagnostic services under PNDT act, GoI in the new Sickle Cell Institute is being planned under the sickle cell institute, VIMSAR Burla.

4 New Born screening for early detection & intervention

To address to high mortality of babies born with sickle cell disease and following up by prophylaxis modality is of high importance for the state. Establishment of Newborn Screening facility for Odisha Sickle Cell Project operational area with follow up prophylactic management for babies with SCD in the state is being planned under the sickle cell institute, VIMSAR Burla.

7

Establishing DNA sequencing/ RNA research unit at the sickle cell institute, VIMSAR Burla

State of art laboratory set up with DNA sequencing facility and research on RNA is being planned under the sickle cell institute, VIMSAR Burla.