Analysis of Tween Based Micro Emulsion in the Presence
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Transcript of Analysis of Tween Based Micro Emulsion in the Presence
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` rifampicin has low solubility and bioavailability inboth fixed and single dose formulation
` factors- pH, absorption and metabolismcontributes to low bioavailability; hencetherapeutic activity affected
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` improves solubility and bioavailability of rifampicin
` protect against enzymatic hydrolysis
` enhances absorption due to surfactant inducepermeability and increase mobility
`
SMEDDS (self-microemulsifying drug deliverysystem) contains oil, & increases solubility,dissolution rate and bioavailability of drug
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` have different structures- (o/w, w/o)
` this helps in releasing the drug
` release of drug rapid for hydrophilic drug (o/w)
` diffusion difficult when in w/o trapped in water droplet
` dissolution studies- rifampicin controlled release isexpected from o/w emulsion droplet
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` MATERIALS
` Tween 80
` Rifampicin` Ethanol
` Oleic Acid
` Phosphate Buffer
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` A. Microemulsion Preparation
oleic acid and PB were mixed with Tween 80
ethanol was added
prepared in screw cap glass vials
observations
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` B. Drug incorporation inmicroemulsion
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` C. Microemulsion characterizationi) Optical Tr anspa re ncy
` homogeneity and optical isotropy
` examined by a cross polarizer and visual examination
` precipitation/phase separation
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ii) C e nt r ifugation
` 2 000rpm for 30 min
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iii) pH
` determined at room temperature.
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iv) S olubility S tudi e s
` solubility determinations
` filtration of material/dilution
` absorbance determined
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v) Oil/Buff er pa r tition co- e ffici e nt
` rifampicin dissolved in Oleic Acid
` centrifuged
`
content of rifampicin in aqueous layer assayed
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vi) Dissolution studi e s`
microemulsion containing Rifampicin filled inhard gelatin capsules.` introduced in 50ml PB.` stirred at constant speed of 2 50rpm.` 2 ml withdrawn at
0, 2 ,4,8,10,15, 2 0,30,40,60,80,100,130,160 minintervals.
` same amount of fresh PB added to maintaindissolution volume.
` filtered and drug [ ] determined.
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` study formulate microemulsion of oleicacid/phosphate buffer/tween 80/ethanol
` to investigate its potential as drug delivery system
for rifampicin` microemulsion subjected to several tests` results indicate
i) microemulsion remained
ii) ME-C optimum
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` solubility & partition studies- drug may reside atinterface of oil and aqueous phase
` paticle size analysis- w/o microemusion dropletchanges into o/w emlsion type
` drug enters the pallisade layer situated on the
inner side of droplet- resulting in controlled releaseof drug
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` slow release of rifampicin from formulation
` advantage- can also be used with high drug
concentrations
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` considering the physico- chemical andspectroscopic analysis of microemulsionformulation of rifampicin
` can be used as oral delivery system for rx of TB
` thus, microemulsion systems help in increasing
solubility of highly hydrophobic drug
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` perform different dissolution methods, eg.rotating basket
` patient compliance reduced microemulsiontaken orally & accurate dose cannot bedetermined
` further in vivo studies need to be done todetermine product acceptability
` change in variables- pH & degree of solubilization
` change in temperature- leads to drug optimum