Analysis - Cromlab Analysis_TR.pdf · Steric selectivity, the ability to separate molecules which...

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Analysis - HPLC - Interchim technologyInterchim manufacturing capabilities

Interchim : Core manufacturing competance and family valuesInterchim has over 40 years of manufacturing experience. Our competence has been built upon an unique technological understanding established through the product supply of many of the recognized manufacturers in the chromatographic field for two generations. The family ethos that runs through Interchim forms the cornerstone for the retention of core understanding of market requirement both within the company organization and through long standing partnerships.

Interchims proprietary range of chromatographic solutions are recognized as providing this sector with cutting edge technological advances relative to the current and progessive requirements of the analyst.

InterFine Chemicals, Chemistry Division :• Custom synthesis from mg to Kgs. Innovative and commercially known compounds• Building blocks & Intermediates, heterocycles, boronic acids, screening compounds • Process optimization• Scale up• Flash Chromatography purification • Traditional techniques for purification• Custom made packaging for combi-chem

InterBiotec, BioScience Innovations Division :• Antibody production & labelling• Fluorescent & enzymatic labelling• Micro-plate custom coating service• Specialty development kits• Specialty biochemical reagents

InterBiochrom, Biochromatography Group :

• Antibody immobilization & purification• Affinity & LC purification under client protocols• HPLC & MPLC analysis

Interchrom, Chromatography Division :• HPLC & GC custom column Mfg.• HPLC & GC development method & optimization• Contract laboratory• Buffer Mfg.• Custom SPE column & plate Mfg.

Historical development of Interchim’s chromatography technologies :

2007 StrategyTM 1.7 µm and 2.2 µm Stationary phase ultra fast analysis & high throughput Modulo-cart™ XS HPLC hardware technology for ultra high pressure

2006 RodeoTM

HPLC method optimization through column coupling technology

2005 Uptisphere® StrategyTM

Latest generation of Silica 100 Å – 425 m2/g

2003 UptiBond™ Premium Multi GC applications capillary columns Atoll™ Polymeric sorbent for Solid Phase Extraction (SPE)

2002 Upti-Select kit HPLC method development tool (in collaboration with LETIAM-IUT laboratory in Orsay) Upti-Clean™ 1st ultra pure spherical based silica for SPE PuriFlash™ Flash Chromatography columns & cartridges

2000 Modulo-cart™ Quick Seal State-of-the art HPLC hardware

1997 Upti-prep®

Interchim’s ultra pure based silica Uptisphere®

Third generation HPLC columns & stationary phases

1995 Modulo-cart™ (version 1)

1994 0.5 mm i.d micro columns (in collaboration with Rodhia's MAN service)

1992 Chirachrom™ chiral columns (in collaboration with CNAM’s general chemistry lab)

1985 HPLC Interchrom columns

1975 Glass & Stainless Steel GC packed columns

Please contact your local distributor.com -

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Analysis - HPLC - Interchim technologyHPLC method development

Carotenoid isomers subcritical chromatographic test for C18 HPLC column classification

A test has been developed at LETIAM (IUT Orsay), within the analytical chemistry laboratory, Paris Sud. that discriminates more than ten groups of reverse stationary phases, from an analysis of carotenoid pigments. Stationary phase properties can then be broadly differentiated between each other. Major features can then be compared for over 160 commercial C18 silica based HPLC columns. From mainstay generation phases such as Licrosorb, Kromasil & Partisil to more current developments, such as Uptisphere and Zorbax.

The test demonstrates, in a single analysis, three primary features of the stationary phase.

Hydrophobicity, overall phase retention capacity, reliant on bonding rate and specific surface area. Steric selectivity, the ability to separate molecules which carry differing 3D structures (isomers). Accessibility to residual silanols, relevant for basic compounds.

Contrary to chemometric approaches, this test presents, simply & concisely, through two successive diagrams (highlighted on the following page), the classification of statio-nary phases that exhibit similar chromatographic behavior relative to phases with different chemical properties.This test is performed in subcritical fluid chromatography, a rapid and effective technique offering an inherent value. The important eluting force of the modified subcritical fluid (carbon dioxide & methanol mixture) and its weak viscosity, lead to a reduced molecular kinetic dispersion (narrow peaks) and rapid analysis from high mobile phase flow rate capabilities. Results obtained with this methodology are correlated with those obtained using hydro-organic mobile phases.

Test conditions :1. Injection of a15 μl diluted mixture of partially isomerizated beta-carotene, containing all-trans, 9-cis, 13-cis (main isomer cis ), 15-cis, and zeaxanthin (di-hydroxylated beta-carotene)

2. The selected mobile phase is a mixture of methanol-carbon dioxide (15 : 85, v/v), at 25°C, outlet pressure 15MPa, flow rate : 3ml /min & detection wavelength at 440 nm.

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ResultsStationary phase classification is achieved in a two step process. Step one provides a primary classification through shape recognition and accessibility to polar sites, defi-ning groups in which the stationary phase are placed. Step two examines the impact of hydrophobic characteristics, of a given phase, for a specified group.

Step 1:Primary classification is established from the comparison of the selectivity value of shape recognition & polar accessibility, for each stationary phase.

Chart placement a. X-axisThe selectivity, between the 13-cis isomer (bent form) and the all-trans (linear form) of the beta-carotene, is plotted on X-axis.The cis/trans isomer separation of the beta-carotene is achieved through a 3D discrimination or shape recognition, by the stationary phases of the linear molecule vs the bent molecule. The variation in the tri-dimensional geometry of the two molecules directly results in differing interactions within the stationary phase.

A comparison of the results obtained through these studies, with other recognized tests of known stationary phases (such as NIST 869), provides a comprehensive organization of phases analyzed :

Monomeric phases (mono-layer bonded silica) with low bonding density for cis/trans selectivity from 1 to 1.1 Monomeric phases with high bonding density or partially polymeric ( polymolecular layers bonded silica) from 1.1 to 1.2 Polymeric phases above 1.2

Monomeric phases can be manufactured through bonding mono, di or tri-functional silanes upon a silica without water content. Polymeric phases are obtained from tri-func-tional silanes containing trace water. Typically, polymeric phases show a capacity for the separation of PAH (Polycyclic Aromatic Hydrocarbons) and rigid isomers of different stereochemistries.

b. Y-axis Selectivity plotted on the Y-axis is given by the selectivity of all trans beta-carotene and zeaxanthin. Polar interactions are exhibited between hydroxyls of the zeaxanthin and the residual silanols of the stationary phase and /or additional accessible polar sites eg. (silane for polyfunctional phases, amide, urea, ether-oxide, quaternary am-monium or carbamates for polar embedded phases also called shielded). The higher the selectivity value, the lower this type of interaction is exhibited within the stationary phase. Therefore the less polar groups contained within the phase, the weaker the retention of zeaxanthin.

This selectivity ranges from 0.3 for phases exhibiting polar linked groups, to almost 20 for the most protected phases. Polar site accessibility is significant from 0.3 to 1, moderate from 1 to 5, low from 5 to 10, and very low above 10. Increased selectivity is, in the majority of instances, relati-ve to multiple end-capping treatments (single or combined), providing an indication of residual silanol coverage. Other non end-capped phases, with high bonding density, display high selectivity suggesting a reduced accessibility to the polar sites available on the stationary phase.


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Step 2 :The first step groups columns that will achieve similar separations. Further classification for each group is achieved (4 5,7 & 8 are displayed) from further examination of silanol accessibility relative to hydrophobicity. For each group a separation will be similar but analysis times will differ .This provides a further indication for the optimization of a method, for a given separation.

Group 4

(The hydrophobicity value cannot be represented in this 2-D diagram)

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Group 5

Group 7

Group 8



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Test consideration for polar linked alkyl & polar end-capped phases

Polar linked alkyl and polar end-capped phases are designed to analyze basic compounds. The polar linked alkyl phase achieves sharp peak definition whilst the polar end-capped phase can be used with a 100% aqueous mobile phase. A major difference between polar linked alkyl and hydrophilic end-capped phases is a weaker hydrophobicity of the polar embedded phases. The hydrophobicity of the hydrophilic end-capped or sulfonamide phases compares to classical C18. In supercritical chromatography, the lack of water within the mobile phase allows for the polar linked groups and the hydrophilic end-capping to display strong interaction with hydroxyl groups of the zeaxanthin analyte. The strength of these interactions can therefore be evaluated.

Selectivity less than 1 on the X axis indicates an inversion of retention between zeaxanthin and the b-carotene, this is a direct result of strong interaction of the two hydroxyls contained within the zeaxanthin molecule. Amide chemistry display a stronger interaction and subsequent peak inversion than the interaction shown by carbamate functiona-lity. These are subsequently stronger than phases carrying ether chemisty.Polar linked phases interact with water of a hydro-organic mobile phase forming a hydrophilic barrier, stopping basic compounds accessing the residual silanols.

Step 2 representation of hydrophobicity /silanol accessibility places phases in distinct groups. Amide phases form a cluster (group A). Group B displays Carbamate phases. Group C classifies two polar end-capped and the sulfonamide phase. Finally, group D covers ether phase technology.

A few phases exhibit distinctive characteristics. Uptisphere® StrategyTM RP is one of these unique phases.


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Upti-selectTM KitA tool to achieve consistent, reliable method development through a simple 2-step processInterchim's C18 stationary phases have been developed to reflect the criteria outlined by the E. Lesellier et al. Tchapla test. Uptisphere® and Uptisphere® StrategyTM are the brand names for Interchim's proprietary HPLC technology, our stationary phases fall into strategic areas of the primary groups within the classification.

The Interchim Upti-selectTM kit contains a range of 5 proprietary columns, across the classification, for the initiation of column selection for analytical method development.

Upti-selectTM kit : Provides a method to predict and select the optimal column for analysis Eliminates column selection uncertainty Enhances the process toward column optimization Reduces development costs

Place Stationary Phase Group

20 Uptisphere HDO G1027 Uptisphere NEC G351 Uptisphere ODB G464 Uptisphere HSC G5116 Uptisphere TF G13138 Uptisphere PLP G1155 IUptisphere BioP II G4156 Uptisphere BioP I G4157 Uptisphere WOD G4158 Uptisphere WRP G4159 Strategy Pro G3160 Strategy RP G10161 Strategy C18-2 G8162 Strategy C18-3 G5



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Step 1 :Inject sample for analysis and the proprietary kit reference mixture onto the Uptisphere® 5 µm ODB packed column. Determine : - The retention time of a Reference Peak (RP) from the analysis sample. - The retention time of the five probes from the Reference Mixture (RM) - [calibrate the system]

Choose a retention factor for the reference peak (RP) : K’RPAn Excel spread sheet indicates the percentage of methanol and /or acetonitrile required, within the mobile phase, to reach the expected retention factor K’RP on each of the five columns.

Step 2 :Prepare the mobile phase according to step 1. Inject the sample of interest onto the five columns (including ODB column for verification). Choose the column highlighting the most suitable separation. Start column optimization.

Upti-selectTM Kit and Upti-selectTM Kit Pack components

Upti-selectTM kit consists of 5 columns featuring identical dimensions and including all necessary hardware. i.e.

- A Reference column packed with the Uptisphere® 5 µm ODB - Four additional columns with complementary selectivity based upon classification chart. e.g Uptisphere® 5 µm HDO Uptisphere® 5 µm NEC Uptisphere® 5 µm HSC Uptisphere® 5 µm TF - Column connectors, (# CH953820)

Upti-selectTM kit Pack also includes:

- Proprietary Reference Mixture (RM) containing five probes for test calibration - CD-ROM (includes : excel spread sheet for automated organic percentage calculation). - Instruction booklet

Description Sizes P/N

Upti-select Kit™ 50 x 1.0 mm S64060Upti-select Kit™ 50 x 2.0 mm S57950Upti-select Kit™ 150 x 2.0 mm T07670Upti-select Kit™ 250 x 2.0 mm T80410Upti-select Kit™ 50 x 3.0 mm S64050Upti-select Kit™ 150 x 3.0 mm T77680Upti-select Kit™ 50 x 4.6 mm S57930Upti-select Kit™ 100 x 4.6 mm S64100Upti-select Kit™ 150 x 4.6 mm S57920Upti-select Kit™ 250 x 4.6 mm S57900 Upti-select Kit™ Pack 50 x 1.0 mm BB2280Upti-select Kit™ Pack 50 x 2.0 mm BB2270Upti-select Kit™ Pack 150 x 2.0 mm BB2260Upti-select Kit™ Pack 250 x 2.0 mm BB2250Upti-select Kit™ Pack 50 x 3.0 mm BB2240Upti-select Kit™ Pack 150 x 3.0 mm BB2230Upti-select Kit™ Pack 50 x 4.6 mm BB2210Upti-select Kit™ Pack 100 x 4.6 mm BB2200Upti-select Kit™ Pack 150 x 4.6 mm BB2190Upti-select Kit™ Pack 250 x 4.6 mm BB2140 Reference Mixture (RM) 5 ml BB2290 Upti-select Kit™ Upgrade BB2310(includes : Reference Mixture (RM) + CD-rom + instruction booklet)


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Upti-selectTM Kit methodology example : Anti-UV agentsStep 1 :a) The anti-UV agent sample contained the following components :

A : 2-Ethylhexyl-2-Cyano-3.3-Diphenylacrylate B : 2-Ethylhexyl-3-[4-Methoxyphenyl]-2-Propenoate C : 4-t-Butyl-4’-Methoxy-Dibenzoyl-Methane D : 2-Ethylhexyl Salicylate

The sample was injected onto the Uptisphere® 5 µm ODB, 250 x 4.6 mm. Conditions : MeOH-H2O (80/20) - 1ml/mn - 25°C - UV : 238 nm.

Peaks B and C co-eluted and the analysis time was more than 60 mnPeak D was thus chosen as the reference peak (RP)

The retention of peak D and the retention time of the five probes (RM) were introduced into the excel spread sheet.The retention factor of peak D was fixed at 8.The calculation table provided the percentage values of methanol and acetonitrile for each mobile phase for each column to achieve K’RP.

Step 2 : The mobile phase were prepared according to Step 1. The sample of interest was injected onto the 5 kit columns according to the fixed conditions to obtain K’RP = 8 for peak D.

b) The Reference Mixture (RM) was injected under the same conditions onto the same column.



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Chromatograms w/Methanol

Chromatograms w/Acetonitrile

Final validated methodSeparation was optimized using Inter-chim's RodeoTM concept.

A running time of 6.88 mm was esta-blished for the last peak and a resolution of 3.02 for the critical pair (B, D).

Conclusion Uptisphere® 5 µm TF and a methanol phase was identified as the most appropriate column for this application.

ObservationsResults show K’RP very close to 8 for each of the five Uptisphere® columns. [This verifies our log P calculation and column transposition equations are relevant]

- The A, B, C, D elution order is preserved on the HSC, ODB, HDO, and NEC columns. - C and D are inverted on the TF column. - A better separation is observed on Uptisphere® NEC and TF. - The B,C pair achieve a better separation when the column features higher silanol accessibility. - Co-elution occurs on the ODB, HDO, and NEC, columns.


A BC

D

AB

C

D

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Hierarchical Classification Ascendant (HCA)Column Bonding Chemistry ManufacturerZorbax C18 Bidentate AgilentDiscovery RP Amide C16 Polar SupelcoNautilus C18 Polar Macherey NagelSupelcosil ABZ+ C16 Polar SupelcoStability BS C23 C16 Polar CIL-CluzeauPerformance C18 Monolithic MerckSpeedRod C18 Monolithic MerckXterra C18 Polar (Hybrid) WatersNucleosil HD C18 High density Macherey NagelEclipse XDB C18 High density AgilentLuna C18 High density PhenomenexNucleodur C18 C18 (ultra pure) Macherey NagelAcclaim C18 C18 DionexNucléosil AB C18 C18 Macherey NagelNucléodur Pyramid C18 C18 endcapped Hydr Macherey NagelSymmetry Shield C18 Polar WatersUptisphere ODB C18 C18 endcapped InterchimUptisphere TF C18 C18 endcapped polyf. InterchimUptisphere HDO C18 C18 mixed-endcapped InterchimUptisphere HSC C18 C18 endcapped InterchimUptisphere NEC C18 C18 non-endcapped Interchim

Stationary Phase characterization for basic compound analysis

The Laboratory of Pharmaceutical Analytical Chemistry – University of Geneva, Switzerland (LCAP) has established a global reputation for separative method development. Their mandate is to evaluate the fundamental aspects that influence the current drives in HPLC.

'Hot topics' such as monolithic sorbents, sub-2 micron particles and high temperature HPLC ( T°> 60°C) are some of their recent considerations and further information on these studies are available upon request.

LCAP basic compound test

Chemometric tools such as Principal Components Analysis (PCA) or Hierarchical Classification Ascendant (HCA), are used to visualize and subsequently classify column performance relative to similar behavior patterns. Column function is clas-sified, within the LCAP test, by the hydrophobic power and the residual interaction with basic compounds. The classification obtained with the LCAP test is correlated with recognized tests published in the scientific literature. LCAP utilizes compounds similar in structure to those found in the pharmaceutical industry and applies mobile phase conditions used in this environment. This establishes the LCAP test as providing relevant information for column suitability for basic compound analysis and discriminates chromatographic sorbents with similar properties.

A number of stationary phases from different manufacturers were tested with the 7 compound test mix at pH 3 & 7.

Retention, efficiency, asymmetry values were measured and the phases studied were represented according to their similarity with PCA and HCA chemometric tools.

Phases were plotted, within a 2 dimensional setting, relative to residual silanol activity at pH : 7 for the principal components within the mix.

HCA computation subsequently groups stationary phases displaying similar chromatographic behavior whilst retaining the “non visible” information within the PCA representation.

LCAP has developed and established chromatographic tests since 1993 to classify silica based HPLC columns for basic compound separation. Such separations have historically been a complicated process using traditional bonded silica based HPLC sorbent generally due to potential ion exchange interactions that can occur within the conventional pH range. For nearly two decades, manufacturers have attempted to overcome problems associateed with basic compound separation by offering new, chemi-cally modified, chromatographic phases. LCAP's current evaluations revolve around the analysis of a mixture containing 7 basic complementary compounds, that allow the rapid discrimination of phase behavior in working conditions (hydro organic buffered mobile phases at pH 3 and 7) .



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Silanol activity Hydrophobic properties


Ascending Hierarchical Classification

Ascending Hierarchical Classification

ACP 2D - AHC95 % variability

ACP 2D - AHC95 % variability

6 main components 6 main components

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Analysis - HPLC - Interchim TechnologyHPLC Method Optimization- RODEOTM

The RODEOTM conceptThe RODEOTM concept provides a novel approach for HPLC method optimization. It takes advantage of the ability to effectively couple multiple Uptisphere HPLC columns together with couplers from the Uptisphere hardware range (see Uptisphere Modulo-Cart hardware details later in this HPLC section).

The chromatograms on the following page demonstrate, and additional studies have shown, that when working under the same analytical conditions and evaluating at the same stage of analysis, pressure (P), efficiency (N), asymmetry (As), resolution (Rs) and retention factor (k’) are extremely similar between a Modulo-Cart Quick Seal 250mm column and five Modulo-Cart Quick Seal 50 mm columns coupled together irrespective of the column internal diameter.

RODEOTM therefore provides a very simple procedure for the optimization of required column dimension, mobile phase, and appropriate temperature & is discussed further on the following pages.

Method Development - to - Method Optimization Interchim's general guide to selecting the most appropriate stationary phase can be represented as follows :

- For samples primarily containing basic compounds : initiate evaluation using the 3 column Upti-select basic compound development kit.

- Sample containing a mixture of basic, neutral and acidic compounds. Intitiate testing with the broad application Uptisphere Strategy column (5 µm C18-2, 250 x 4.6 mm), conditions : ACN - H2O (70/30) & (30/70). If unsuccessful, apply Upti-select KitTM methodology with standardized phase diversity and/ or apply other selectivities such as cyano, amino, ion exchange.

The appropriate column can then be optimized. Interchim have established the RODEOTM concept highlighted below to address column optimization.

Modulo-cart Quick seal 250 mm

RodeoTM 5 x 50 mm

[Modulo-Cart Quick seal technology exhibits 15% increased efficiency compared to other HPLC hardware. It is a real hand-tight fitting and the carbon doped PEEK on stainless steel guarantees long column lifetime].


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Quick seal UP5HDO 250 x 4.6 mm RodeoTM UP5HDO (5 x 50) x 4.6 mm ACN/H2O 85/151mL/min T° : 23CPesticides : DDOH, Endosulfane, Heptachlor, ppDDT, 44DDMU, op DDT, Aldrin.

Dimension to k’ P As N Rs

250 mm 2.35 6.88 61 1.17 21148 8.585 x 50 mm 2.4 6.8 63 0.99 22574 8.56

Dimension to k’ P As N Rs

250 mm 2.44 1.12 92 1.18 15875 3.75 x 50 mm 2.47 1.13 107 1.2 16200 3.9

Quick seal UP5TF 250 x 2.1 mm RodeoTM UP5TF (5 x 50) x 2.1 mm

ACN/H2O 70/30 0.2mL/min - T° : 23CTest interne : Uracil, toluene, naphtalene.

Analysis - HPLC - Interchim TechnologyHPLC Method Optimization - RODEOTM

Exploring the difference between a 250 mm Modulo-cart column and five 50 mm columns coupled together with Modulo-cart couplers.

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RODEOTM column length optimization

The selection of a HPLC stationary phase is typically determined in the laboratory through the initial evaluation of a 5 µm, 250 mm column. The RodeoTM concept provides a means to quickly select the correct column length and reach the required optimisation. Optimization sets to reduce analysis time whilst retaining sufficient resolution for peaks displaying the poorest separations. The principal objective is to shorten run time, re-equilibration time, and identify the simplest compatible mobile phase. A way in reaching this objective is to adopt the appropriate column length.

Example 1 displays this process for RodeoTM [partical size reduction is then considered as a subsequent step]. The three subsequent chromatograms show the affect when a column is removed (de-coupled) for the same separation. In this instance it shows that by decreasing the column length by 40%, analysis time is reduced by 42.5% whilst sufficient resolution is retained for the critical pair separation.

RodeoTM UP5HDO (5 x 50) x 4.6 mm

Name Tr As N Rs

DDOH 4.87 1,08 23050 0Endosulfane 9.11 1.01 22608 22.93Heptachlor 12.32 1.01 23561 11.39ppDDT 12.91 0.99 23692 1.8144DDMU 13.55 1 24027 1.84op DDT 14.85 0.99 22455 3.5Aldrin 18.74 0.99 22574 8.68

Analysis - HPLC - Interchim technologyHPLC Method Optimization - RODEOTM


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Name Tr As N Rs

DDOH 3.85 1.09 17499 0Endosulfane 7.23 1.03 18122 20.43Heptachlor 9.78 1.02 17585 10ppDDT 10.27 1 17624 1.6244DDMU 10.8 1.02 18611 1.7op DDT 11.84 1 17732 3.08Aldrin 14.95 1 17986 7.76


Name Tr As N Rs

DDOH 2.8 1.08 12906 0Endosulfane 5.23 1.02 13392 17.44Heptachlor 7.06 1.04 13824 8.69ppDDT 7.39 1.01 15796 1.3744DDMU 7.76 1.01 13864 1.48op DDT 8.52 1.01 13345 2.73Aldrin 10.77 0.99 13398 6.75


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Mobile phase & RODEOTM column length optimization

Example 2. UP5HDO 50 x 4.6 mm :

fig2. UP5HDO 50 x 4.6 mm : ACN /H2O (75/25)

Name Tr As N Rs

DDOH 1.29 1.07 4509 0Endosulfane 3.06 1.04 4929 14.05Heptachlor 4.58 1.04 4728 6.9144DDMU 5.19 0.94 3899 2.04op DDT 5.79 1.01 4699 1.8Aldrin 7.33 1 4764 4.03

The following studies show that an optimization step through a modification of mobile phase will have less impact than compared to optimization through selection of the appropriate column length. It is particularly significant when eluting conditions are fixed or predetermined.

fig1. Exhibits a separation of a mixture of pesticides from an Uptisphere 5 μm C18-HDO, 50 x 4.6 mm column. The mobile phase set as ACN /H2O (95/5) leads to Heptachlor, 44DDMU & opDDT colelution.

fig2. Shows results when we increase the percentage of water within the mobile phase. All peaks are separated with a run time of 7.33 min

fig3. Shows results when we increase the length of the column. Peaks exhibit a sufficient resolution (> 1.5) and the final run time is 5.14 min

fig1.


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fig3. UP5HDO 3 x 50mm (coupled) x 4.6 mm : ACN /H2O (95/5)

Name Tr As N Rs

DDOH 2.22 1.05 12845 0Endosulfane 3.12 1.01 11895 9.27Heptachlor 3.68 1.01 12631 4.5744DDMU 3.9 1 12050 1.67op DDT 4.2 0.96 11682 1.97Aldrin 5.14 0.98 12712 5.58

Description Dimension P/N

RODEOTM Strategy 5 C18-2 5 x 50 x 4.6 mm US1740RODEOTM Uptisphere 5 ODB 5 x 50 x 4.6 mm BZ9130RODEOTM Uptisphere 5 HDO 5 x 50 x 4.6 mm BZ9150RODEOTM Uptisphere 5 NEC 5 x 50 x 4.6 mm BZ9170RODEOTM Uptisphere 5 HSC 5 x 50 x 4.6 mm BZ9210RODEOTM Uptisphere 5 TF 5 x 50 x 4.6 mm BZ9190 RODEOTM Strategy 5 C18-2 5 x 50 x 2.0 mm US1750RODEOTM Uptisphere 5 ODB 5 x 50 x 2.0 mm BZ9140RODEOTM Uptisphere 5 HDO 5 x 50 x 2.0 mm BZ9160RODEOTM Uptisphere 5 NEC 5 x 50 x 2.0 mm BZ9180RODEOTM Uptisphere 5 HSC 5 x 50 x 2.0 mm BZ9220RODEOTM Uptisphere 5 TF 5 x 50 x 2.0 mm BZ9200

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Analysis - HPLC - Interchim technologyHPLC Method Optimization

Further considerations Mobile phase, temperature and pH optimization are explored below through an optimization of the anti-UV agent separation originally presented earlier in the method development section utilizing the Upti-select KitTM. In this example Uptisphere® TF was identified as the preferred column. Final peak retention time was 24 mn with 1.35 of resolution for the critical pair (B,D).

[It is also worth paying attention to simple HPLC system checks for your application type. i.e. dead volumes such as tubings, fittings etc ; check the detection cell volume, the injection loop volume and adapt the detector response time].

A modification of the organic concentration and temperature, finally achieves a run time of 6.88 min with a resolution of 3.02 for the critical pair (fig1).

Original Separation of anti-UV agent by Uptisphere® TF

Followed by a subsequent variation in % methanol in the mobile phase...


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Analysis - HPLC - Interchim technologyHPLC Method Optimization

and a final variation in temperature (fig1.) ...

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Analysis - HPLC - Interchim technologyHPLC Method Optimization - OSIRIS™

OSIRIS™ - Software for HPLC method development & optimizationOSIRIS™ software is designed for the development and optimization of the analysis conditions in liquid chromatography and can generate a robust method from just a few preliminary analysis. OSIRIS™ allows, within isocratic or gradient elution mode, to optimize the conditions of elution (mobile phase content, temperature, pH). The latest version 4.0 of the software provides a new easy to use interface and a step by step guide through the optimization process.

OSIRIS™ provides efficient, accurate optimization through management of the analytical variables i.e.

Mobile phase composition (isocratic, binary, ternary or quaternary, linear and multi-linear gradients), pH and /or temperature Multidimensional optimization ; isocratic composition (binary or ternary) / temperature - binary isocratic composition /pH - gradient /temperature - gradient /pH

Optimize

OSIRIS™ takes account of your main criteria and enhances method and subsequent performance.

Establish your own requirement criteria in terms of separation quality (resolution), analysis time, and/or analysis condition robustness and identify the sensitivity of method to small variations in working parameters. OSIRIS™ also allows you to focus on the separation of specific compounds within a mixture.

ValidateOSIRIS™ is an excellent tool for validation and transfer of HPLC method taking account of the analytical variables. Physical properties of columns can also be studied with this software. The OSIRIS™ simulation table provides a means to compare chromatograms in different conditions of analysis.

Description P/N

OSIRIS™ , HPLC optimization software CC9260


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Analysis - HPLC - Interchim technologyHPLC Method development

Additional Uptipshere kits

Method DevelopmentThe kit consists of 3 HPLC columns from the Uptipshere® and Uptisphere® StrategyTM range that adhere to your considered requirements. Column phases need to be speci-fied on the order.

Description Micron Dimension P/N

Method development kit 3 µm 150 x 4.6 mm DEV031546Method development kit 5 µm 250 x 4.6 mm DEV052546Method development kit 3 µm 150 x 3.0 mm DEV031530Method development kit 5 µm 250 x 3.0 mm DEV052530Method development kit 3 µm 150 x 2.0 mm DEV031520Method development kit 5 µm 250 x 2.0 mm DEV052520

Method Validation

The kit consists of 3 HPLC columns with the same dimensions from the Uptipshere® and Uptisphere® StrategyTM range that adhere to your considered requirements. Column phases need to be specified on the order. Every column is manufactured with a different stationary phase batch.

Description Micron Dimension P/N

Method validation kit 3 µm 150 x 4.6 mm VAL031546Method validation kit 5 µm 250 x 4.6 mm VAL052546

Analytical to Preparative DevelopmentThe kit consists of your chosen preparative column and an analytical column of same length with 4.6 mm internal diameter packed with the same phase batch. This kit is manufactured upon request. Contact your local distributor or Interchim's technical center for further information.

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Analysis - HPLC - Interchim technologyUpti-prep® silica technology

Upti-prep® is Interchim's proprietary silica technology that is utilized within our range of HPLC, prep LC, SPE and Flash Chromatography products, the Upti-prep® technology has been developed around rigorous, innovative manufacturing processes from the highest quality raw material. Upti-prep® based silica are manufactured in ceramic reactors from particles exempt of metal trace content. Every step of each synthesis is strictly controlled establishing particles that display extreme mechanical stability. Particle size, porosity & specific surface area exhibit reproducibility and clear definition, achieving a perfectly hydroxylated surface and cylindrical pores.

Upti-prep® technology establishes major advantages that subsequently assist the separation process:

1. A weaker surface energy compared to first generation silicas (A type) and a number of second generation silicas (B type). This ultimately facilitates narrow peaks with basic compounds.2. A very high quantity of free silanols combined with excellent accessibility leads to homogeneous & dense bonding chemistry. This establishes very high loading capacities and stability of the subsequent stationary phase under aggressive mobile phase conditions such as basic buffers.

Upti-clean™, Solid Phase Extraction

Uptisphere®, Uptisphere Strategy™Analytical and prep HPLC

puriFlash™, Flash Chromatography purification

Silica : Ultra pure (99,995 %)

Shape : spherical

Particle size : 1.7 µm [+/- 0,1] 2.2 µm [+/- 0,15] 3 µm [+/- 0,2] 5 µm [+/- 0,3] 10 µm [+/- 1,0] 15 µm [+/- 2,0] 50 µm [+/- 5,0]

Surface area / Pore size: 60 Å [+/- 10] / 500 m2/g [+/- 50] 100 Å [+/- 15] / 425 m2/g [+/- 40] 120 Å [+/- 15] / 320 m2/g [+/- 40] 300 Å [+/- 40] / 100 m2/g [+/- 20] 500 Å [+/- 55] / 60 m2/g [+/- 10]

Metal content : Uptisphere® < 20 ppm (Fe < 1 ppm) Strategy™ < 10 ppm

pH stability : 1.5 to 7.5(Some of our stationary phases are stable between pH 1 to 12)


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Analysis - HPLC - Interchim technologyUpti-prep® Silica technology

2. Guaiacol evaluation

1.0 < Pyridine Symmetry (10%) < 1.15

1.0 < Guaiacol Symmetry (10%) < 1.15

Suggested applications :

Analytical Services• Sample preparation• Method developmemnt and validation• Stability studies• QA/QC• Biological Monitoring

Drug Discovery LabChemical Development LabMédicinal Chemistry LabPurification – Process• Identification• Purity testing• Purification

Characteristics

As a second pre-cautionary measure, an independent company has evaluated the features of our silica under different conditions of use. It was demonstrated that our range of silica can endure multiple packing & unpacking without damaging the sorbent integrity - a primary preparative requirement.

Pyridine and guaiacol (zeolithe content) are the principal probes Interchim utilize to test for inertness and control each and every silica batch we produce

1. Pyridine evaluation

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Analysis - HPLC - Interchim technologyColumn Hardware - Modulo-cart

Modulo-cart® - Interchim's hardware of choice for their HPLC rangeInterchim recognize that HPLC hardware is an integral component of a high quality HPLC column.

Extensive time and resources have been dedicated to develop our series of Modulo-cart HPLC column hardware for the Uptisphere® HPLC column range. Each series within the range are precision engineered and provide distinct advantages compared to alterative hardware systems found on the market in terms of ease of use and column performance.

Modulo-cart developments are progressively driven by the application demands of the market. An overview of the hardware range is highlighted on the following page.

The Modulo-cart Quick-Seal (QS) system is the central system within the Uptisphere® hardware range and is utilized in the majority of the Uptisphere® columns for analytical requirement. The distinct engineering attributes of the QS series are explored later in this section.

A 'K' designation on a series of column hardware highlights that a range of guard columns is available within the series. For example : Within the Modulo-cart Quick-Seal series, columns supplied with designation 'QK' consist of the analytical column with an integrated guard holder + one packed guard cartridge.

Modulo-cart XS

Modulo-cart Nano LC

Modulo-cart QS

Modulo-cart QK

Modulo-cart Prep


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Analysis - HPLC - Interchim technologyColumn Hardware - Modulo-cart Quick Seal

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Analysis - HPLC - Interchim technologyColumn Hardware - Modulo-cart Quick Seal

Modulo-cart Quick Seal HardwareThe Modulo-cart Quick Seal (QS) hardware system provides up to 15% gains in column efficiency compared to competitor systems. QS systems can operate at pressures greater than 800 bar and are compatible for use in super critical fluid chromatography. The extensive attention to detail of this cartridge based system and the precision engineering is highlighted in the pictures below.

Column i.d. (mm) Tubing i.d. (inches) Flow rate (ml/min)

1.0 - 2.1 0.005 0.05 - 0.22.1 - 3.0 0.005 - 0.007 0.20 - 0.53.0 - 3.9 - 4.0 - 4.6 0.007 - 0.010 0.50 - 1.04.6 - 6.0 - 7.8 - 9.4 - 10 0.010 2.0 - 1010.0 - 21.2 0.010 - 0.020 10 - 2550 0.020 - 0.045 50 - 100

Capillary port

Thread

Modulo-cart Quick Seal Competitor

Modulo-cart Quick Seal Competitor

Additional benefits :

Hand-tight fitting Direct connection between the guard cartridge and the analytical column Encapsulated frits are easy to remove No drying during storage time The carbon spiked PEEK on stainless steel guarantees a long life time

The Modulo-Cart Quick Seal is available in lengths ranging from 33 -to- 300mm and internal diameters from 1.0 -to- 4.6mm.


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Analysis - HPLC - Interchim technologyModulo-cart Quick seal

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Description P/N Qty

QS Column connectors CH953820 2 / pk

QS Column coupler CH882060 1

LC /MS SurefitTM connector50 µm i.d. T80590 1

Guard Cartridge holderDirect connection for Modulo-cart QS (10 mm) CH797540 1

1/16”- 1/16” standard CH980740 1

Column accessoriesHighlighted below are a list of the most common accessories required for the Modulo-cart hardware.

Analysis - HPLC - Interchim technologyModulo-cart Quick Seal Accessories

Description P/N Qty

Guard cartridges 10 x 1.0 mm Q95961 310 x 1.0 mm Q95960 1010 x 2.0 mm CH980671 310 x 2.0 mm CH980670 1010 x 4.0 mm CH979521 310 x 4.0 mm CH979520 10

Caps/frits 2µm for 1.0 mm i.d CH980600 5for 2.0 mm i.d CH881940 5for 3.0 mm i.d CH881950 5for 3.9 mm i.d CH881960 5for 4.0 mm i.d CH881960 5for 4.6 mm i.d CH881970 5

Frit replacement tool CH347520 1

On-line filter0.5 µm T50270 102.0 µm R21281 10

C-clamps (Easy Modulo-Cart tighten /untightening) Column i.d. 1.0 & 2.0 mm T94280 13.0 - 4.0 - 4.6 mm T49870 1


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Analysis - HPLC - Interchim technologyC18 Uptisphere® stationary phases & QS columns

Uptisphere® and Uptisphere® StrategyTM C18 stationary phases

Interchim manufactured their first Uptisphere® stationary phase almost 15 years ago. The development was seen as a natural progression as Interchim had established an unique technological understanding of HPLC requirement through the product supply of many of the recognized HPLC brands both within manufacturers hardware and within Interchim's Interchrom HPLC range that utilizes the benefits associated with Interchim packing expertize and Modulo-cart hardware.

Uptisphere® ODB and HDO were Interchim's first stationary phases. They are referenced in a multitude of HPLC analytical methods throughout the pharmaceutical sector and beyond. Interchim's initial manufacturing directive was to establish consistent column to column reproducibility - through an intensive management of stationary phase production, the packing process and column hardware.

Our long standing on-going working relationship with the Molecular Analysis Instruments and Technique studies laboratory (LETIAM), a part of the Paris Sud analytical che-mistry group located at the Orsay IUT, has heavily influenced our thoughts on subsequent C18 stationary phase development.

The LETIAM are globally cited as providing HPLC expertize, particularly relative to silica sorbent characterization, developing a simple, rapid test from carotenoid pigment analysis to compare three principle criteria of stationary phase i.e.

Hydrophobicity (related to the carbon content) Shape recognition (dependent on bonding density, bonding type & specific surface area) Polar interaction site accessibility (relative to the base silica, end-capping, or the type of bonded C18)

Uptisphere® and Uptisphere® StrategyTM bonded C18 stationary phases have been manufactured to compliment the criteria defined by this test. This is discussed in greater detail within the 'HPLC method development' section at the beginning of this chapter. Additional collaborations with other scientific laboratories have assisted in the tailoring of our phase development and improve the accuracy and capabilities of our chosen chemistries.

Stationary Phase

Uptisphere® stationary phases are manufactured from Upti-prep® 120Å silica. The more recently developed Uptisphere® StrategyTM phases utilize Upti-prep® 100Å silica.

Synthesis

Interchim adopt four principal processes for the synthesis of their range of stationary phase i.e :

Mono functional + 'one step' end-capping Mono functional + 'multi step' end-capping Mono functional + 'mixed' end-capping Poly functional + 'one step' end-capping

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Uptisphere® & Uptisphere® StrategyTM are a complimentary family of C18 phases manufactured to provide the analyst with a diverse phase selection that target a broad spectra of applications. Manufacture has been guided by method development studies highlighted earlier in this chapter and exhibit tailored hydrophobicity and silanol acces-sibility within a given parameter of phase selectivity.

Uptisphere® C18-ODBHistorically our C18-phase of reference for method development. Serves a broad-ship of analytical requirements for separating mid to non polar compounds.

Uptisphere® C18-HDOThe final steps of the HDO manufacturing process creates a selectivity for mid-polar compounds in 100% aqueous mobile phase. C18-HDO has no hydrophilic end-capping and is not a PLAP type phase.

Uptisphere® C18-NECDisplays characteristics similar to ODB without the end-capping. NEC strongly retains the polar and mid-polar compounds from the solvent front. Today most pharmaceutical compounds contains chains and /or carbon cycles combined with numerous polar groups and are generally more basic in character. Such compounds create a tailing effect on classical C18 phases, C18-NEC can overcome this characteristic in the majority of cases.

Uptisphere® C18-HSCHSC displays excellent selectivity for non-polar compounds thanks to Interchim's proprietary end-capping process.

Uptisphere® C18-TFTF provides an alternative selectivity to classical existing phases and has been adopted for challenging separations. C18-TF is a polyfunctional end-capped C18, and is recommended for - but not limited to - aromatic, polyphenol, PAHs etc.

Uptisphere® StrategyTM C18-2Seen by Interchim as a progessive development to Uptisphere® ODB, this utility phase serves many pharmaceutical applications. StrategyTM C18-2 has a 425 m2/g surface area providing excellent loading capacity capabilities. Uptisphere® StrategyTM C18-3The high bonding density of C18-3 facilitiates a stronger separation of non polar compounds. Multi step bonding technology guarantees a fully end-capped phase, stable under basic pH conditions. C18-3 is an excellent phase for the integral separation of basic drugs up to pH : 12.

Uptisphere® StrategyTM RPStrategyTM RP mixed type proprietary end-capping establishes this phase as suitable for extreme polar compound separation. RP shows excellent mechanical stability under 100% aqueous mobile phase condition.


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Phase Bonding Pore size Å Surface area m2/g % C end-capping USP code

Strategy C18-2 C18 100 425 19 multi step L1Strategy C18-3 C18 100 425 22 multi step L1Strategy RP C18 100 425 16 mixed L1Uptisphere ODB C18 120 320 18 one step L1Uptisphere HDO C18 120 320 17 mixed L1Uptisphere NEC C18 120 320 16 non L1Uptisphere HSC C18 120 320 20 multi step L1Uptisphere TF t C18 120 320 14 one step L1

1. Each phase batch undergoes strict quality control.2. Every Modulo-Cart Quick Seal column is individually tested and delivered with its own chromatogram certificate.3. Every Modulo-Cart Quick Seal produced fits the companies stringent standards of production4. Every Modulo-Cart Quick Seal is shipped within 24 working hours of receipt of order

Interchim column guarantee

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Phase Particle size Dimension Modulo-Cart QS Modulo-Cart QK

Strategy C18-2 3 µm 100 x 2.0 mm US0730 US0930Strategy C18-2 3 µm 150 x 2.0 mm US0740 US0940Strategy C18-2 5 µm 100 x 2.0 mm US0750 US0950Strategy C18-2 5 µm 150 x 2.0 mm US0760 US0960Strategy C18-2 5 µm 250 x 2.0 mm US0770 US0970Strategy C18-2 3 µm 150 x 3.0 mm US0700 US0900Strategy C18-2 5 µm 150 x 3.0 mm US0710 US0910Strategy C18-2 5 µm 250 x 3.0 mm US0720 US0920Strategy C18-2 3 µm 125 x 4.0 mm US0640 US0840Strategy C18-2 3 µm 150 x 4.0 mm US0650 US0850Strategy C18-2 5 µm 125 x 4.0 mm US0660 US0860Strategy C18-2 5 µm 150 x 4.0 mm US0670 US0870Strategy C18-2 5 µm 250 x 4.0 mm US0680 US0880Strategy C18-2 10 µm 250 x 4.0 mm US0690 US0890Strategy C18-2 3 µm 100 x 4.6 mm US0580 US0780Strategy C18-2 3 µm 150 x 4.6 mm US0590 US0790Strategy C18-2 5 µm 100 x 4.6 mm US0600 US0800Strategy C18-2 5 µm 150 x 4.6 mm US0610 US0810Strategy C18-2 5 µm 250 x 4.6 mm US0620 US0820Strategy C18-2 10 µm 250 x 4.6 mm US0630 US0830

Strategy C18-3 3 µm 100 x 2.0 mm US1970 US2170Strategy C18-3 3 µm 150 x 2.0 mm US1980 US2180Strategy C18-3 5 µm 100 x 2.0 mm US1990 US2190Strategy C18-3 5 µm 150 x 2.0 mm US2000 US2200Strategy C18-3 5 µm 250 x 2.0 mm US2010 US2210Strategy C18-3 3 µm 150 x 3.0 mm US1940 US2140Strategy C18-3 5 µm 150 x 3.0 mm US1950 US2150Strategy C18-3 5 µm 250 x 3.0 mm US1960 US2160Strategy C18-3 3 µm 125 x 4.0 mm US1880 US2080Strategy C18-3 3 µm 150 x 4.0 mm US1890 US2090Strategy C18-3 5 µm 125 x 4.0 mm US1900 US2100Strategy C18-3 5 µm 150 x 4.0 mm US1910 US2110Strategy C18-3 5 µm 250 x 4.0 mm US1920 US2120Strategy C18-3 10 µm 250 x 4.0 mm US1930 US2130Strategy C18-3 3 µm 100 x 4.6 mm US1820 US2020Strategy C18-3 3 µm 150 x 4.6 mm US1830 US2030Strategy C18-3 5 µm 100 x 4.6 mm US1840 US2040Strategy C18-3 5 µm 150 x 4.6 mm US1850 US2050Strategy C18-3 5 µm 250 x 4.6 mm US1860 US2060Strategy C18-3 10 µm 250 x 4.6 mm US1870 US2070

Uptisphere® StrategyTM

Octadecyl - USP code L1100 Å - 425 m2

%C : 19mono functional + "multi-step" end capping

Selectivity 13 cis/b-carotene all trans : 1.11Selectivity b-carotene/Zeaxanthine : 11Hydrophobicity b-carotene all trans : 11.4

Octadecyl - USP code L1100 Å - 425 m2/g%C : 22mono functional + "multi-step" end-capping

Selectivity 13 cis/b-carotene all trans : 1.07Selectivity b-carotene/Zeaxanthine : 12.66Hydrophobicity b-carotene all trans : 21.15

Modulo-cart informationpage B.26 - B.30

Uptisphere® HPLC Columns


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Strategy RP 3 µm 100 x 2.0 mm US0260 US0400Strategy RP 3 µm 150 x 2.0 mm US0270 US0410Strategy RP 5 µm 100 x 2.0 mm US0060 US0130Strategy RP 5 µm 150 x 2.0 mm US0050 US0120Strategy RP 5 µm 250 x 2.0 mm US0040 US0110Strategy RP 3 µm 150 x 3.0 mm US0230 US0370Strategy RP 5 µm 150 x 3.0 mm US0240 US0380Strategy RP 5 µm 250 x 3.0 mm US0250 US0390Strategy RP 3 µm 125 x 4.0 mm US0170 US0310Strategy RP 3 µm 150 x 4.0 mm US0180 US0320Strategy RP 5 µm 125 x 4.0 mm US0190 US0330Strategy RP 5 µm 150 x 4.0 mm US0200 US0340Strategy RP 5 µm 250 x 4.0 mm US0210 US0350Strategy RP 10 µm 250 x 4.0 mm US0220 US0360Strategy RP 3 µm 100 x 4.6 mm US0140 US0280Strategy RP 3 µm 150 x 4.6 mm US0150 US0290Strategy RP 5 µm 100 x 4.6 mm US0020 US0100Strategy RP 5 µm 150 x 4.6 mm US0010 US0090Strategy RP 5 µm 250 x 4.6 mm US0000 US0080Strategy RP 10 µm 250 x 4.6 mm US0160 US0300 ODB 3 µm 100 x 2.0 mm UP3ODB#10QS UP3ODB#10QKODB 3 µm 125 x 2.0 mm UP3ODB#12QS UP3ODB#12QKODB 3 µm 150 x 2.0 mm UP3ODB#15QS UP3ODB#15QKODB 5 µm 100 x 2.0 mm UP5ODB#10QS UP5ODB#10QKODB 5 µm 125 x 2.0 mm UP5ODB#12QS UP5ODB#12QKODB 5 µm 150 x 2.0 mm UP5ODB#15QS UP5ODB#15QKODB 5 µm 250 x 2.0 mm UP5ODB#25QS UP5ODB#25QKODB 3 µm 100 x 3.0 mm UP3ODB$10QS UP3ODB$10QKODB 3 µm 125 x 3.0 mm UP3ODB$12QS UP3ODB$12QKODB 3 µm 150 x 3.0 mm UP3ODB$15QS UP3ODB$15QKODB 5 µm 100 x 3.0 mm UP5ODB$10QS UP5ODB$10QKODB 5 µm 125 x 3.0 mm UP5ODB$12QS UP5ODB$12QKODB 5 µm 150 x 3.0 mm UP5ODB$15QS UP5ODB$15QKODB 5 µm 250 x 3.0 mm UP5ODB$25QS UP5ODB$25QKODB 3 µm 100 x 4.0 mm UP3ODB*10QS UP3ODB*10QKODB 3 µm 125 x 4.0 mm UP3ODB*12QS UP3ODB*12QKODB 3 µm 150 x 4.0 mm UP3ODB*15QS UP3ODB*15QKODB 5 µm 100 x 4.0 mm UP5ODB*10QS UP5ODB*10QKODB 5 µm 125 x 4.0 mm UP5ODB*12QS UP5ODB*12QKODB 5 µm 150 x 4.0 mm UP5ODB*15QS UP5ODB*15QKODB 5 µm 250 x 4.0 mm UP5ODB*25QS UP5ODB*25QKODB 5 µm 300 x 4.0 mm UP5ODB*30QS UP5ODB*30QKODB 10 µm 250 x 4.0 mm UP10ODB*25QS UP10ODB*25QKODB 10 µm 300 x 4.0 mm UP10ODB*30QS UP10ODB*30QKODB 3 µm 100 x 4.6 mm UP3ODB-10QS UP3ODB-10QKODB 3 µm 125 x 4.6 mm UP3ODB-12QS UP3ODB-12QKODB 5 µm 150 x 4.6 mm UP3ODB-15QS UP3ODB-15QKODB 5 µm 100 x 4.6 mm UP5ODB-10QS UP5ODB-10QKODB 5 µm 125 x 4.6 mm UP5ODB-12QS UP5ODB-12QKODB 5 µm 150 x 4.6 mm UP5ODB-15QS UP5ODB-15QKODB 5 µm 200 x 4.6 mm UP5ODB-20QS UP5ODB-20QKODB 5 µm 250 x 4.6 mm UP5ODB-25QS UP5ODB-25QKODB 10 µm 250 x 4.6 mm UP10ODB-25QS UP10ODB-25QK



Octadecyl - USP code L1120Å - 320 m2/g%C : 18mono functional + "one step" end-capping

Octadecyl - USP code L1100Å - 425 m2/g%C : 16mono functional + "mixed" end-capping

Selectivity 13 cis/b-carotene all trans : 1Selectivity b-carotene/Zeaxanthine : 8.3Hydrophobicity b-carotene all trans : 23.66


Uptisphere®

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HDO 3 µm 100 x 2.0 mm UP3HDO#10QS UP3HDO#10QKHDO 3 µm 125 x 2.0 mm UP3HDO#12QS UP3HDO#12QKHDO 3 µm 150 x 2.0 mm UP3HDO#15QS UP3HDO#15QKHDO 5 µm 100 x 2.0 mm UP5HDO#10QS UP5HDO#10QKHDO 5 µm 125 x 2.0 mm UP5HDO#12QS UP5HDO#12QKHDO 5 µm 150 x 2.0 mm UP5HDO#15QS UP5HDO#15QKHDO 5 µm 250 x 2.0 mm UP5HDO#25QS UP5HDO#25QKHDO 3 µm 100 x 3.0 mm UP3HDO$10QS UP3HDO$10QKHDO 3 µm 125 x 3.0 mm UP3HDO$12QS UP3HDO$12QKHDO 3 µm 150 x 3.0 mm UP3HDO$15QS UP3HDO$15QKHDO 5 µm 100 x 3.0 mm UP5HDO$10QS UP5HDO$10QKHDO 5 µm 125 x 3.0 mm UP5HDO$12QS UP5HDO$12QKHDO 5 µm 150 x 3.0 mm UP5HDO$15QS UP5HDO$15QKHDO 5 µm 250 x 3.0 mm UP5HDO$25QS UP5HDO$25QKHDO 3 µm 100 x 4.0 mm UP3HDO*10QS UP3HDO*10QKHDO 3 µm 125 x 4.0 mm UP3HDO*12QS UP3HDO*12QKHDO 3 µm 150 x 4.0 mm UP3HDO*15QS UP3HDO*15QKHDO 5 µm 100 x 4.0 mm UP5HDO*10QS UP5HDO*10QKHDO 5 µm 125 x 4.0 mm UP5HDO*12QS UP5HDO*12QKHDO 5 µm 150 x 4.0 mm UP5HDO*15QS UP5HDO*15QKHDO 5 µm 250 x 4.0 mm UP5HDO*25QS UP5HDO*25QKHDO 5 µm 300 x 4.0 mm UP5HDO*30QS UP5HDO*30QKHDO 10 µm 250 x 4.0 mm UP10HDO*25QS UP10HDO*25QKHDO 10 µm 300 x 4.0 mm UP10HDO*30QS UP10HDO*30QKHDO 3 µm 100 x 4.6 mm UP3HDO-10QS UP3HDO-10QKHDO 3 µm 125 x 4.6 mm UP3HDO-12QS UP3HDO-12QKHDO 3 µm 150 x 4.6 mm UP3HDO-15QS UP3HDO-15QKHDO 5 µm 100 x 4.6 mm UP5HDO-10QS UP5HDO-10QKHDO 5 µm 125 x 4.6 mm UP5HDO-12QS UP5HDO-12QKHDO 5 µm 150 x 4.6 mm UP5HDO-15QS UP5HDO-15QKHDO 5 µm 200 x 4.6 mm UP5HDO-20QS UP5HDO-20QKHDO 5 µm 250 x 4.6 mm UP5HDO-25QS UP5HDO-25QKHDO 10 µm 250 x 4.6 mm UP10HDO-25QS UP10HDO-25QK


Uptisphere®



Uptisphere® HPLC Columns cont.



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NEC 3 µm 100 x 2.0 mm UP3NEC#10QS UP3NEC#10QKNEC 3 µm 125 x 2.0 mm UP3NEC#12QS UP3NEC#12QKNEC 3 µm 150 x 2.0 mm UP3NEC#15QS UP3NEC#15QKNEC 5 µm 100 x 2.0 mm UP5NEC#10QS UP5NEC#10QKNEC 5 µm 125 x 2.0 mm UP5NEC#12QS UP5NEC#12QKNEC 5 µm 150 x 2.0 mm UP5NEC#15QS UP5NEC#15QKNEC 5 µm 250 x 2.0 mm UP5NEC#25QS UP5NEC#25QKNEC 3 µm 100 x 3.0 mm UP3NEC$10QS UP3NEC$10QKNEC 3 µm 125 x 3.0 mm UP3NEC$12QS UP3NEC$12QKNEC 3 µm 150 x 3.0 mm UP3NEC$15QS UP3NEC$15QKNEC 5 µm 100 x 3.0 mm UP5NEC$10QS UP5NEC$10QKNEC 5 µm 125 x 3.0 mm UP5NEC$12QS UP5NEC$12QKNEC 5 µm 150 x 3.0 mm UP5NEC$15QS UP5NEC$15QKNEC 5 µm 250 x 3.0 mm UP5NEC$25QS UP5NEC$25QKNEC 3 µm 100 x 4.0 mm UP3NEC*10QS UP3NEC*10QKNEC 3 µm 125 x 4.0 mm UP3NEC*12QS UP3NEC*12QKNEC 3 µm 150 x 4.0 mm UP3NEC*15QS UP3NEC*15QKNEC 5 µm 100 x 4.0 mm UP5NEC*10QS UP5NEC*10QKNEC 5 µm 125 x 4.0 mm UP5NEC*12QS UP5NEC*12QKNEC 5 µm 150 x 4.0 mm UP5NEC*15QS UP5NEC*15QKNEC 5 µm 250 x 4.0 mm UP5NEC*25QS UP5NEC*25QKNEC 5 µm 300 x 4.0 mm UP5NEC*30QS UP5NEC*30QKNEC 3 µm 100 x 4.6 mm UP3NEC-10QS UP3NEC-10QKNEC 3 µm 125 x 4.6 mm UP3NEC-12QS UP3NEC-12QKNEC 3 µm 150 x 4.6 mm UP3NEC-15QS UP3NEC-15QKNEC 5 µm 100 x 4.6 mm UP5NEC-10QS UP5NEC-10QKNEC 5 µm 125 x 4.6 mm UP5NEC-12QS UP5NEC-12QKNEC 5 µm 150 x 4.6 mm UP5NEC-15QS UP5NEC-15QKNEC 5 µm 200 x 4.6 mm UP5NEC-20QS UP5NEC-20QKNEC 5 µm 250 x 4.6 mm UP5NEC-25QS UP5NEC-25QK


Uptisphere®

Octadecyl - USP code L1120Å - 320 m2/g%C : 16mono functional + non end-capped


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HSC 3 µm 100 x 2.0 mm UP3HSC#10QS UP3HSC#10QKHSC 3 µm 125 x 2.0 mm UP3HSC#12QS UP3HSC#12QKHSC 3 µm 150 x 2.0 mm UP3HSC#15QS UP3HSC#15QKHSC 5 µm 100 x 2.0 mm UP5HSC#10QS UP5HSC#10QKHSC 5 µm 125 x 2.0 mm UP5HSC#12QS UP5HSC#12QKHSC 5 µm 150 x 2.0 mm UP5HSC#15QS UP5HSC#15QKHSC 5 µm 250 x 2.0 mm UP5HSC#25QS UP5HSC#25QKHSC 3 µm 100 x 3.0 mm UP3HSC$10QS UP3HSC$10QKHSC 3 µm 125 x 3.0 mm UP3HSC$12QS UP3HSC$12QKHSC 3 µm 150 x 3.0 mm UP3HSC$15QS UP3HSC$15QKHSC 5 µm 100 x 3.0 mm UP5HSC$10QS UP5HSC$10QKHSC 5 µm 125 x 3.0 mm UP5HSC$12QS UP5HSC$12QKHSC 5 µm 150 x 3.0 mm UP5HSC$15QS UP5HSC$15QKHSC 5 µm 250 x 3.0 mm UP5HSC$25QS UP5HSC$25QKHSC 3 µm 100 x 4.0 mm UP3HSC*10QS UP3HSC*10QKHSC 3 µm 125 x 4.0 mm UP3HSC*12QS UP3HSC*12QKHSC 3 µm 150 x 4.0 mm UP3HSC*15QS UP3HSC*15QKHSC 5 µm 100 x 4.0 mm UP5HSC*10QS UP5HSC*10QKHSC 5 µm 125 x 4.0 mm UP5HSC*12QS UP5HSC*12QKHSC 5 µm 150 x 4.0 mm UP5HSC*15QS UP5HSC*15QKHSC 5 µm 250 x 4.0 mm UP5HSC*25QS UP5HSC*25QKHSC 5 µm 300 x 4.0 mm UP5HSC*30QS UP5HSC*30QKHSC 3 µm 100 x 4.6 mm UP3HSC-10QS UP3HSC-10QKHSC 3 µm 125 x 4.6 mm UP3HSC-12QS UP3HSC-12QKHSC 3 µm 150 x 4.6 mm UP3HSC-15QS UP3HSC-15QKHSC 5 µm 100 x 4.6 mm UP5HSC-10QS UP5HSC-10QKHSC 5 µm 125 x 4.6 mm UP5HSC-12QS UP5HSC-12QKHSC 5 µm 150 x 4.6 mm UP5HSC-15QS UP5HSC-15QKHSC 5 µm 200 x 4.6 mm UP5HSC-20QS UP5HSC-20QKHSC 5 µm 250 x 4.6 mm UP5HSC-25QS UP5HSC-25QK TF 5 µm 100 x 2.0 mm UP5TF#10QS UP5TF#10QKTF 5 µm 125 x 2.0 mm UP5TF#12QS UP5TF#12QKTF 5 µm 150 x 2.0 mm UP5TF#15QS UP5TF#15QKTF 5 µm 250 x 2.0 mm UP5TF#25QS UP5TF#25QKTF 5 µm 100 x 3.0 mm UP5TF$10QS UP5TF$10QKTF 5 µm 125 x 3.0 mm UP5TF$12QS UP5TF$12QKTF 5 µm 150 x 3.0 mm UP5TF$15QS UP5TF$15QKTF 5 µm 250 x 3.0 mm UP5TF$25QS UP5TF$25QKTF 5 µm 100 x 4.0 mm UP5TF*10QS UP5TF*10QKTF 5 µm 125 x 4.0 mm UP5TF*12QS UP5TF*12QKTF 5 µm 150 x 4.0 mm UP5TF*15QS UP5TF*15QKTF 5 µm 250 x 4.0 mm UP5TF*25QS UP5TF*25QKTF 5 µm 100 x 4.6 mm UP5TF-10QS UP5TF-10QKTF 5 µm 125 x 4.6 mm UP5TF-12QS UP5TF-12QKTF 5 µm 150 x 4.6 mm UP5TF-15QS UP5TF-15QKTF 5 µm 200 x 4.6 mm UP5TF-20QS UP5TF-20QKTF 5 µm 250 x 4.6 mm UP5TF-25QS UP5TF-25QK

Uptisphere®

Octadecyl - USP code L1120Å - 320 m2/g%C : 20mono functional + "multi step" end-capping


Octadecyl - USP code L1%C : 14poly functional + "one step" end-capping

Selectivity 13 cis/b-carotene all trans : 1.205Selectivity b-carotene/Zeaxanthine : 2.5Hydrophobicity b-carotene all trans : 9




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Analysis - HPLC - Interchim technologyPLP Uptisphere® stationary phases & columns

Uptisphere® PLP,Reverse phase with polar linked groupThe Uptisphere® PLP stationary phase has a polar group embedded within a hydrophobic chain.

PLP exhibits :

100% compatibility with aqueous mobile phases Excellent peak symmetry with basic compounds Very good retention of polar compounds

Uptisphere® PLP is available in 5 µm and is stable between pH 2.5-to-7.5

Phase Particle size Dimension Modulo-Cart HS

PLP 5 µm 20 x 4.6 mm UP5PLP-2HS PLP 5 µm 33 x 4.6 mm UP5PLP-3HS PLP 5 µm 50 x 4.6 mm UP5PLP-5HS PLP 5 µm 75 x 4.6 mm UP5PLP-7HS PLP 5 µm 33 x 4.0 mm UP5PLP*3HS PLP 5 µm 50 x 3.0 mm UP5PLP$5HS PLP 5 µm 20 x 2.0 mm UP5PLP#2HS PLP 5 µm 33 x 2.0 mm UP5PLP#3HS PLP 5 µm 50 x 2.0 mm AUP5PLP#5HS Phase Particle size Dimension Modulo-Cart QS Modulo-Cart QK

PLP 5 µm 100 x 2.0 mm UP5PLP#10QS UP5PLP#10QKPLP 5 µm 125 x 2.0 mm UP5PLP#12QS UP5PLP#12QKPLP 5 µm 150 x 2.0 mm UP5PLP#15QS UP5PLP#15QKPLP 5 µm 250 x 2.0 mm UP5PLP#25QS UP5PLP#25QKPLP 5 µm 100 x 3.0 mm UP5PLP$10QS UP5PLP$10QKPLP 5 µm 125 x 3.0 mm UP5PLP$12QS UP5PLP$12QKPLP 5 µm 150 x 3.0 mm UP5PLP$15QS UP5PLP$15QKPLP 5 µm 250 x 3.0 mm UP5PLP$25QS UP5PLP$25QKPLP 5 µm 100 x 4.0 mm UP5PLP*10QS UP5PLP*10QKPLP 5 µm 125 x 4.0 mm UP5PLP*12QS UP5PLP*12QKPLP 5 µm 150 x 4.0 mm UP5PLP*15QS UP5PLP*15QKPLP 5 µm 250 x 4.0 mm UP5PLP*25QS UP5PLP*25QKPLP 5 µm 100 x 4.6 mm UP5PLP-10QS UP5PLP-10QKPLP 5 µm 125 x 4.6 mm UP5PLP-12QS UP5PLP-12QKPLP 5 µm 150 x 4.6 mm UP5PLP-15QS UP5PLP-15QKPLP 5 µm 200 x 4.6 mm UP5PLP-20QS UP5PLP-20QKPLP 5 µm 250 x 4.6 mm UP5PLP-25QS UP5PLP-25QK

Uptisphere 5 PLP, 250 x 4.6 mmMobile Phase : (15/85) MeOH/H2O [50mM] phosphate (pH2.31) Flow rate : 1mL/min Temperature : 25 °CDetect.: UV (254nm)

Compound k' As

1 Barbituric acid 0.06 1.1 2 Codeine 0.33 0,93 Atropine 0.40 #4 Papaverine 1.77 1.015 Noscapine 2.1 1.016 Diphenylhydramine 1.34 1.13

Uptisphere® PLP

Polar linked reverse phaseUSP code L1%C : 14poly functional + end-capping "one step"


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Separation of strong basic drugs on Uptisphere® StrategyTM Hilic5 µm, 250 x 4.6 mm

MeOH - Buffer pH : 6.3 (70/30)

tr As

Amitryptilin 6.55 1.15Propanolol 9.05 1.16

Analysis - HPLC - Interchim technologyUptisphere® StrategyTM Hilic stationary phases & columns

Upti-prep® technology establishes major advantages that subsequently assist the separation process:

1. A weaker surface energy compared to first generation silicas (A type) and a number of second generation silicas (B type). This ultimately facilitates narrow peaks with basic compounds.2. A very high quantity of free silanols combined with excellent accessibility leads to homogeneous & dense bonding chemistry. This establishes very high loading capacities and stability of the subsequent stationary phase under aggressive mobile phase conditions such as basic buffers.

An optimized version - 100Å - 450 m2/g - has been developed for Hilic separation

Columns are shipped under 100% Ethanol

Mobile phase should contain at least 40% organic and 5% polar solvent.pH stability : 1 to 7.5


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Dimensions P/N

Modulo-cart HS StrategyTM 5 µm Hilic SI 30 x 4.6 mm US5540 50 x 4.6 mm US5550 50 x 3.0 mm US5560 33 x 2.0 mm US5570 50 x 2.0 mm US5580

Modulo-cart QS StrategyTM 5 µm Hilic SI 100 x 4.6 mm US5380 150 x 4.6 mm US5390 250 x 4.6 mm US5400 150 x 3.0 mm US5410 250 x 3.0 mm US5420 100 x 2.0 mm US5430 150 x 2.0 mm US5440 250 x 2.0 mm US5450

Modulo-cart QK StrategyTM 5 µm Hilic SI 100 x 4.6 mm US5460 150 x 4.6 mm US5470 250 x 4.6 mm US5480 150 x 3.0 mm US5490 250 x 3.0 mm US5500 100 x 2.0 mm US5510 150 x 2.0 mm US5520 250 x 2.0 mm US5530

Analysis - HPLC - Interchim technologyUptisphere® StrategyTM Hilic stationary phases & columns

QS QK


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Analysis - HPLC - Interchim technologyAdditional Uptisphere® stationary phases & QS columns

Additional Uptisphere® and Uptisphere® StrategyTM stationary phases

Fifteen additional chemstries are offered within the Uptisphere® range to complement the C18 Uptisphere® offering. They support a variety of analyte to stationary phase interaction.

All Uptisphere® phases are manufactured using Upti-prepTM silica technology as detailed earlier in this chapter. Uptisphere® stationary phases are made using Upti-prepTM 120Ǻ silica; whilst Uptisphere® StrategyTM phases are manufactured on Upti-prepTM 100Ǻ silica.

Available chemistries are detailed in the table below.

Phase Bonding Pore size Å Surface area m2/g % C end-capping USP code

Strategy C8-2 C8 100 425 14 one step L7Strategy SI OH 100 425 non L4Uptisphere C8 C8 120 320 11 one step L7Uptisphere C8U C8 120 320 6 non L7Uptisphere C4 C4 120 320 7 one step L26Uptisphere CN Cyano 120 320 8 one step L10Uptisphere PH phenyl 120 320 9 one step L11Uptisphere NH2 amino 120 320 11 non L8Uptisphere SCX Exchange cations 120 320 non L50Uptisphere MM1 C8/SCX 120 320 non L44Uptisphere SAX Exchange anions 120 320 non L14Uptisphere MM3 C8/SAX 120 320 non L28Uptisphere SI OH 120 320 non L4Uptisphere OH diol 120 320 non L20Uptisphere DNAP DNAP 120 320 non


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C8-2 3 µm 100 x 2.0 mm US2550 US2750C8-2 3 µm 150 x 2.0 mm US2560 US2760C8-2 5 µm 100 x 2.0 mm US2570 US2770C8-2 5 µm 150 x 2.0 mm US2580 US2780C8-2 5 µm 250 x 2.0 mm US2590 US2790C8-2 3 µm 150 x 3.0 mm US2520 US2720C8-2 5 µm 150 x 3.0 mm US2530 US2730C8-2 5 µm 250 x 3.0 mm US2540 US2740C8-2 3 µm 125 x 4.0 mm US2460 US2660C8-2 3 µm 150 x 4.0 mm US2470 US2670C8-2 5 µm 125 x 4.0 mm US2480 US2680C8-2 5 µm 150 x 4.0 mm US2490 US2690C8-2 5 µm 250 x 4.0 mm US2500 US2700C8-2 10 µm 250 x 4.0 mm US2510 US2710C8-2 3 µm 100 x 4.6 mm US2400 US2600C8-2 3 µm 150 x 4.6 mm US2410 US2610C8-2 5 µm 100 x 4.6 mm US2420 US2620C8-2 5 µm 150 x 4.6 mm US2430 US2630C8-2 5 µm 250 x 4.6 mm US2440 US2640C8-2 10 µm 250 x 4.6 mm US2450 US2650 SI 5 µm 100 x 2.0 mm US1550 US1680SI 5 µm 150 x 2.0 mm US1560 US1690SI 5 µm 250 x 2.0 mm US1570 US1700SI 5 µm 150 x 3.0 mm US1530 US1660SI 5 µm 250 x 3.0 mm US1540 US1670SI 5 µm 125 x 4.0 mm US1490 US1620SI 5 µm 150 x 4.0 mm US1500 US1630SI 5 µm 250 x 4.0 mm US1510 US1640SI 10 µm 250 x 4.0 mm US1520 US1650SI 5 µm 100 x 4.6 mm US1450 US1580SI 5 µm 150 x 4.6 mm US1460 US1590SI 5 µm 250 x 4.6 mm US1470 US1600SI 10 µm 250 x 4.6 mm US1380 US1610


Octyl - (USP code L7)100Å - 425 m2/g%C : 14mono functional + "one step" end-capping

tr As NTriterbutylbenzene 3.05 1.24 19105Diethylphtalate 4.92 1.07 20437

Silica - (USP code L4)100Å - 425 m2/g


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C8 3 µm 100 x 2.0 mm UP3C8#10QS UP3C8#10QKC8 3 µm 125 x 2.0 mm UP3C8#12QS UP3C8#12QKC8 3 µm 150 x 2.0 mm UP3C8#15QS UP3C8#15QKC8 5 µm 100 x 2.0 mm UP5C8#10QS UP5C8#10QKC8 5 µm 125 x 2.0 mm UP5C8#12QS UP5C8#12QKC8 5 µm 150 x 2.0 mm UP5C8#15QS UP5C8#15QKC8 5 µm 250 x 2.0 mm UP5C8#25QS UP5C8#25QKC8 3 µm 100 x 3.0 mm UP3C8$10QS UP3C8$10QKC8 3 µm 125 x 3.0 mm UP3C8$12QS UP3C8$12QKC8 3 µm 150 x 3.0 mm UP3C8$15QS UP3C8$15QKC8 5 µm 100 x 3.0 mm UP5C8$10QS UP5C8$10QKC8 5 µm 125 x 3.0 mm UP5C8$12QS UP5C8$12QKC8 5 µm 150 x 3.0 mm UP5C8$15QS UP5C8$15QKC8 5 µm 250 x 3.0 mm UP5C8$25QS UP5C8$25QKC8 3 µm 100 x 4.0 mm UP3C8*10QS UP3C8*10QKC8 3 µm 125 x 4.0 mm UP3C8*12QS UP3C8*12QKC8 3 µm 150 x 4.0 mm UP3C8*15QS UP3C8*15QKC8 5 µm 100 x 4.0 mm UP5C8*10QS UP5C8*10QKC8 5 µm 125 x 4.0 mm UP5C8*12QS UP5C8*12QKC8 5 µm 150 x 4.0 mm UP5C8*15QS UP5C8*15QKC8 5 µm 250 x 4.0 mm UP5C8*25QS UP5C8*25QKC8 10 µm 250 x 4.0 mm UP10C8*25QS UP10C8*25QKC8 3 µm 100 x 4.6 mm UP3C8-10QS UP3C8-10QKC8 3 µm 125 x 4.6 mm UP3C8-12QS UP3C8-12QKC8 3 µm 150 x 4.6 mm UP3C8-15QS UP3C8-15QKC8 5 µm 100 x 4.6 mm UP5C8-10QS UP5C8-10QKC8 5 µm 125 x 4.6 mm UP5C8-12QS UP5C8-12QKC8 5 µm 150 x 4.6 mm UP5C8-15QS UP5C8-15QKC8 5 µm 200 x 4.6 mm UP5C8-20QS UP5C8-20QKC8 5 µm 250 x 4.6 mm UP5C8-25QS UP5C8-25QKC8 10 µm 250 x 4.6 mm UP10C8-25QS UP10C8-25QK

Uptisphere®

Octyl - (USP code L7)120Å - 320 m2/g%C : 11mono functional + "one step" end-capping



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C8U 5 µm 100 x 2.0 mm UP5C8U#10QS UP5C8U#10QKC8U 5 µm 125 x 2.0 mm UP5C8U#12QS UP5C8U#12QKC8U 5 µm 150 x 2.0 mm UP5C8U#15QS UP5C8U#15QKC8U 5 µm 250 x 2.0 mm UP5C8U#25QS UP5C8U#25QKC8U 5 µm 100 x 3.0 mm UP5C8U$10QS UP5C8U$10QKC8U 5 µm 125 x 3.0 mm UP5C8U$12QS UP5C8U$12QKC8U 5 µm 150 x 3.0 mm UP5C8U$15QS UP5C8U$15QKC8U 5 µm 250 x 3.0 mm UP5C8U$25QS UP5C8U$25QKC8U 5 µm 100 x 4.0 mm UP5C8U*10QS UP5C8U*10QKC8U 5 µm 125 x 4.0 mm UP5C8U*12QS UP5C8U*12QKC8U 5 µm 150 x 4.0 mm UP5C8U*15QS UP5C8U*15QKC8U 5 µm 250 x 4.0 mm UP5C8U*25QS UP5C8U*25QKC8U 5 µm 100 x 4.6 mm UP5C8U-10QS UP5C8U-10QKC8U 5 µm 125 x 4.6 mm UP5C8U-12QS UP5C8U-12QKC8U 5 µm 150 x 4.6 mm UP5C8U-15QS UP5C8U-15QKC8U 5 µm 200 x 4.6 mm UP5C8U-20QS UP5C8U-20QKC8U 5 µm 250 x 4.6 mm UP5C8U-25QS UP5C8U-25QK C4 3 µm 100 x 2.0 mm UP3C4#10QS UP3C4#10QKC4 3 µm 125 x 2.0 mm UP3C4#12QS UP3C4#12QKC4 3 µm 150 x 2.0 mm UP3C4#15QS UP3C4#15QKC4 5 µm 100 x 2.0 mm UP5C4#10QS UP5C4#10QKC4 5 µm 125 x 2.0 mm UP5C4#12QS UP5C4#12QKC4 5 µm 150 x 2.0 mm UP5C4#15QS UP5C4#15QKC4 5 µm 250 x 2.0 mm UP5C4#25QS UP5C4#25QKC4 3 µm 100 x 3.0 mm UP3C4$10QS UP3C4$10QKC4 3 µm 125 x 3.0 mm UP3C4$12QS UP3C4$12QKC4 3 µm 150 x 3.0 mm UP3C4$15QS UP3C4$15QKC4 5 µm 100 x 3.0 mm UP5C4$10QS UP5C4$10QKC4 5 µm 125 x 3.0 mm UP5C4$12QS UP5C4$12QKC4 5 µm 150 x 3.0 mm UP5C4$15QS UP5C4$15QKC4 5 µm 250 x 3.0 mm UP5C4$25QS UP5C4$25QKC4 3 µm 100 x 4.0 mm UP3C4*10QS UP3C4*10QKC4 3 µm 125 x 4.0 mm UP3C4*12QS UP3C4*12QKC4 3 µm 150 x 4.0 mm UP3C4*15QS UP3C4*15QKC4 5 µm 100 x 4.0 mm UP5C4*10QS UP5C4*10QKC4 5 µm 125 x 4.0 mm UP5C4*12QS UP5C4*12QKC4 5 µm 150 x 4.0 mm UP5C4*15QS UP5C4*15QKC4 5 µm 250 x 4.0 mm UP5C4*25QS UP5C4*25QKC4 3 µm 100 x 4.6 mm UP3C4-10QS UP3C4-10QKC4 3 µm 125 x 4.6 mm UP3C4-12QS UP3C4-12QKC4 3 µm 150 x 4.6 mm UP3C4-15QS UP3C4-15QKC4 5 µm 100 x 4.6 mm UP5C4-10QS UP5C4-10QKC4 5 µm 125 x 4.6 mm UP5C4-12QS UP5C4-12QKC4 5 µm 150 x 4.6 mm UP5C4-15QS UP5C4-15QKC4 5 µm 200 x 4.6 mm UP5C4-20QS UP5C4-20QKC4 5 µm 250 x 4.6 mm UP5C4-25QS UP5C4-25QK

Uptisphere®

Octyl - (USP code L7)120Å - 320 m2/g%C : 6mono functional + non end-capped

Butyl - (USP code L26)120Å - 320 m2/g%C : 7mono functional + "one step" end-capping


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CN 3 µm 100 x 2.0 mm UP3CN#10QS UP3CN#10QKCN 3 µm 125 x 2.0 mm UP3CN#12QS UP3CN#12QKCN 3 µm 150 x 2.0 mm UP3CN#15QS UP3CN#15QKCN 5 µm 100 x 2.0 mm UP5CN#10QS UP5CN#10QKCN 5 µm 125 x 2.0 mm UP5CN#12QS UP5CN#12QKCN 5 µm 150 x 2.0 mm UP5CN#15QS UP5CN#15QKCN 5 µm 250 x 2.0 mm UP5CN#25QS UP5CN#25QKCN 3 µm 100 x 3.0 mm UP3CN$10QS UP3CN$10QKCN 3 µm 125 x 3.0 mm UP3CN$12QS UP3CN$12QKCN 3 µm 150 x 3.0 mm UP3CN$15QS UP3CN$15QKCN 5 µm 100 x 3.0 mm UP5CN$10QS UP5CN$10QKCN 5 µm 125 x 3.0 mm UP5CN$12QS UP5CN$12QKCN 5 µm 150 x 3.0 mm UP5CN$15QS UP5CN$15QKCN 5 µm 250 x 3.0 mm UP5CN$25QS UP5CN$25QKCN 3 µm 100 x 4.0 mm UP3CN*10QS UP3CN*10QKCN 3 µm 125 x 4.0 mm UP3CN*12QS UP3CN*12QKCN 3 µm 150 x 4.0 mm UP3CN*15QS UP3CN*15QKCN 5 µm 100 x 4.0 mm UP5CN*10QS UP5CN*10QKCN 5 µm 125 x 4.0 mm UP5CN*12QS UP5CN*12QKCN 5 µm 150 x 4.0 mm UP5CN*15QS UP5CN*15QKCN 5 µm 250 x 4.0 mm UP5CN*25QS UP5CN*25QKCN 10 µm 250 x 4.0 mm UP10CN*25QS UP10CN*25QKCN 3 µm 100 x 4.6 mm UP3CN-10QS UP3CN-10QKCN 3 µm 125 x 4.6 mm UP3CN-12QS UP3CN-12QKCN 3 µm 150 x 4.6 mm UP3CN-15QS UP3CN-15QKCN 5 µm 100 x 4.6 mm UP5CN-10QS UP5CN-10QKCN 5 µm 125 x 4.6 mm UP5CN-12QS UP5CN-12QKCN 5 µm 150 x 4.6 mm UP5CN-15QS UP5CN-15QKCN 5 µm 200 x 4.6 mm UP5CN-20QS UP5CN-20QKCN 5 µm 250 x 4.6 mm UP5CN-25QS UP5CN-25QKCN 10 µm 250 x 4.6 mm UP10CN-25QS UP10CN-25QK PH 5 µm 100 x 2.0 mm UP5PH#10QS UP5PH#10QKPH 5 µm 125 x 2.0 mm UP5PH#12QS UP5PH#12QKPH 5 µm 150 x 2.0 mm UP5PH#15QS UP5PH#15QKPH 5 µm 250 x 2.0 mm UP5PH#25QS UP5PH#25QKPH 5 µm 100 x 3.0 mm UP5PH$10QS UP5PH$10QKPH 5 µm 125 x 3.0 mm UP5PH$12QS UP5PH$12QKPH 5 µm 150 x 3.0 mm UP5PH$15QS UP5PH$15QKPH 5 µm 250 x 3.0 mm UP5PH$25QS UP5PH$25QKPH 5 µm 100 x 4.0 mm UP5PH*10QS UP5PH*10QKPH 5 µm 125 x 4.0 mm UP5PH*12QS UP5PH*12QKPH 5 µm 150 x 4.0 mm UP5PH*15QS UP5PH*15QKPH 5 µm 250 x 4.0 mm UP5PH*25QS UP5PH*25QKPH 5 µm 100 x 4.6 mm UP5PH-10QS UP5PH-10QKPH 5 µm 125 x 4.6 mm UP5PH-12QS UP5PH-12QKPH 5 µm 150 x 4.6 mm UP5PH-15QS UP5PH-15QKPH 5 µm 200 x 4.6 mm UP5PH-20QS UP5PH-20QKPH 5 µm 250 x 4.6 mm UP5PH-25QS UP5PH-25QK


Uptisphere®

Cyano - (USP code L10)120Å - 320 m2/g%C : 8mono functional + "one step" end-capping

Phényl - (USP code L11)120Å - 320 m2/g%C : 9mono functional + "one step" end-capping


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NH2 5 µm 150 x 2.0 mm UP5NH2#15QS UP5NH2#15QKNH2 5 µm 250 x 2.0 mm UP5NH2#25QS UP5NH2#25QKNH2 5 µm 150 x 3.0 mm UP5NH2$15QS UP5NH2$15QKNH2 5 µm 250 x 3.0 mm UP5NH2$25QS UP5NH2$25QKNH2 5 µm 150 x 4.0 mm UP5NH2*15QS UP5NH2*15QKNH2 5 µm 250 x 4.0 mm UP5NH2*25QS UP5NH2*25QKNH2 5 µm 150 x 4.6 mm UP5NH2-15QS UP5NH2-15QKNH2 5 µm 250 x 4.6 mm UP5NH2-25QS UP5NH2-25QK SCX 5 µm 250 x 4.0 mm UP5SCX*25QS UP5SCX*25QKSCX 10 µm 250 x 4.0 mm UP10SCX*25QS UP10SCX*25QKSCX 5 µm 250 x 4.6 mm UP5SCX-25QS UP5SCX-25QKSCX 10 µm 250 x 4.6 mm UP10SCX-25QS UP10SCX-25QK MM1 5 µm 250 x 4.6 mm UP5MM1-25QS UP5MM1-25QKMM1 5 µm 250 x 4.0 mm UP5MM1*25QS UP5MM1*25QK SAX 5 µm 250 x 4.0 mm UP5SAX*25QS UP5SAX*25QKSAX 10µm 250 x 4.0 mm UP10SAX*25QS UP10SAX*25QKSAX 5 µm 250 x 4.6 mm UP5SAX-25QS UP5SAX-25QKSAX 10 µm 250 x 4.6 mm UP10SAX-25QS UP10SAX-25QK MM3 5 µm 250 x 4.6 mm UP5MM3-25QS UP5MM3-25QKMM3 5 µm 250 x 4.0 mm UP5MM3*25QS UP5MM3*25QK

Uptisphere®

Amino - (USP code L8)120Å - 320 m2/g%C : 5mono functional non end-capped

Strong Cation exchanger(USP code L50) - 120Å - 320 m2/g

Octyl / Strong Cation exchanger (USP code L44) - 120Å - 320 m2/g

Strong Anion exchanger (USP code L14) - 120Å - 320 m2/g

Octyl / Strong Anion exchanger(USP code L28) - 120Å - 320 m2/g



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SI 3 µm 100 x 2.0 mm UP3SI#10QS UP3SI#10QKSI 3 µm 125 x 2.0 mm UP3SI#12QS UP3SI#12QKSI 3 µm 150 x 2.0 mm UP3SI#15QS UP3SI#15QKSI 5 µm 100 x 2.0 mm UP5SI#10QS UP5SI#10QKSI 5 µm 125 x 2.0 mm UP5SI#12QS UP5SI#12QKSI 5 µm 150 x 2.0 mm UP5SI#15QS UP5SI#15QKSI 5 µm 250 x 2.0 mm UP5SI#25QS UP5SI#25QKSI 3 µm 100 x 3.0 mm UP3SI$10QS UP3SI$10QKSI 3 µm 125 x 3.0 mm UP3SI$12QS UP3SI$12QKSI 3 µm 150 x 3.0 mm UP3SI$15QS UP3SI$15QKSI 5 µm 100 x 3.0 mm UP5SI$10QS UP5SI$10QKSI 5 µm 125 x 3.0 mm UP5SI$12QS UP5SI$12QKSI 5 µm 150 x 3.0 mm UP5SI$15QS UP5SI$15QKSI 5 µm 250 x 3.0 mm UP5SI$25QS UP5SI$25QKSI 3 µm 100 x 4.0 mm UP3SI*10QS UP3SI*10QKSI 3 µm 125 x 4.0 mm UP3SI*12QS UP3SI*12QKSI 3 µm 150 x 4.0 mm UP3SI*15QS UP3SI*15QKSI 5 µm 100 x 4.0 mm UP5SI*10QS UP5SI*10QKSI 5 µm 125 x 4.0 mm UP5SI*12QS UP5SI*12QKSI 5 µm 150 x 4.0 mm UP5SI*15QS UP5SI*15QKSI 5 µm 250 x 4.0 mm UP5SI*25QS UP5SI*25QKSI 5 µm 300 x 4.0 mm UP5SI*30QS UP5SI*30QKSI 10 µm 250 x 4.0 mm UP10SI*25QS UP10SI*25QKSI 10 µm 300 x 4.0 mm UP10SI*30QS UP10SI*30QKSI 3 µm 100 x 4.6 mm UP3SI-10QS UP3SI-10QKSI 3 µm 125 x 4.6 mm UP3SI-12QS UP3SI-12QKSI 3 µm 150 x 4.6 mm UP3SI-15QS UP3SI-15QKSI 5 µm 100 x 4.6 mm UP5SI-10QS UP5SI-10QKSI 5 µm 125 x 4.6 mm UP5SI-12QS UP5SI-12QKSI 5 µm 150 x 4.6 mm UP5SI-15QS UP5SI-15QKSI 5 µm 200 x 4.6 mm UP5SI-20QS UP5SI-20QKSI 5 µm 250 x 4.6 mm UP5SI-25QS UP5SI-25QKSI 10 µm 250 x 4.6 mm UP10SI-25QS UP10SI-25QK OH 6 µm 150 x 2.0 mm UP6OH#15QS UP6OH#15QKOH 6 µm 250 x 2.0 mm UP6OH#25QS UP6OH#25QKOH 6 µm 150 x 3.0 mm UP6OH$15QS UP6OH$15QKOH 6 µm 250 x 3.0 mm UP6OH$25QS UP6OH$25QKOH 6 µm 150 x 4.0 mm UP6OH*15QS UP6OH*15QKOH 6 µm 250 x 4.0 mm UP6OH*25QS UP6OH*25QKOH 6 µm 150 x 4.6 mm UP6OH-15QS UP6OH-15QKOH 6 µm 250 x 4.6 mm UP6OH-25QS UP6OH-25QK DNAP 5 µm 250 x 4.6 mm UP5DNAP-25QS UP5DNAP-25QK


Uptisphere®


Diol - (USP code L20)120Å - 320 m2/g%C : 6

Dinitroanilidopropyl120Å - 320 m2/g




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Analysis - HPLC - Interchim technologyUptisphere® stationary phases & HS columns

Modulo-Cart HSReduce analysis time whilst maintaining resolution

Modulo-Cart HS columns are designed to achieve, within a smaller length format, rapid and efficient analysis.Interchim have established tailored packing processes for their 3 µm and 5 µm Uptisphere® and Uptisphere® StrategyTM stationary phases within short column lengths to maximize subsequent short column performance and lifetime. The HS range is available in 1.0, 2.0 and 4.6mm i.d and 20, 25, 33, 50 and 75 mm lengths.

Dedicated to rapid analysis Ideal for rapid gradients and LC/MS Zero dead volume

Considerations toward enhancing column performanceCare should be taken to remove the additional column dead volumes to maximize the performance of Modulo-cart HS columns . The tube connection between column and the injector must have a 0.005” diameter. It is also important to reduce the rotor valve volume of the injector and to use a nano volume cell within the detector.

5 µm, 250 x 4.6 mm 3 µm, 20 x 4.6 mm

1,5 min



Modulo-cart HS need to be used w/ QS connectors CH953820

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C18-2 3 µm 20 x 2.0 mm US1100C18-2 3 µm 33 x 2.0 mm US1110C18-2 3 µm 50 x 2.0 mm US1120C18-2 5 µm 20 x 2.0 mm US1130C18-2 5 µm 33 x 2.0 mm US1140C18-2 5 µm 50 x 2.0 mm US1150C18-2 3 µm 50 x 3.0 mm US1080C18-2 5 µm 50 x 3.0 mm US1090C18-2 3 µm 33 x 4.0 mm US1060C18-2 5 µm 33 x 4.0 mm US1070C18-2 3 µm 20 x 4.6 mm US0980C18-2 3 µm 33 x 4.6 mm US0990C18-2 3 µm 50 x 4.6 mm US1000C18-2 3 µm 75 x 4.6 mm US1010C18-2 5 µm 20 x 4.6 mm US1020C18-2 5 µm 33 x 4.6 mm US1030C18-2 5 µm 50 x 4.6 mm US1040C18-2 5 µm 75 x 4.6 mm US1050 C18-3 3 µm 20 x 2.0 mm US2340C18-3 3 µm 33 x 2.0 mm US2350C18-3 3 µm 50 x 2.0 mm US2360C18-3 5 µm 20 x 2.0 mm US2370C18-3 5 µm 33 x 2.0 mm US2380C18-3 5 µm 50 x 2.0 mm US2390C18-3 3 µm 50 x 3.0 mm US2320C18-3 5 µm 50 x 3.0 mm US2330C18-3 3 µm 33 x 4.0 mm US2300C18-3 5 µm 33 x 4.0 mm US2310C18-3 3 µm 20 x 4.6 mm US2220C18-3 3 µm 33 x 4.6 mm US2230C18-3 3 µm 50 x 4.6 mm US2240C18-3 3 µm 75 x 4.6 mm US2250C18-3 5 µm 20 x 4.6 mm US2260C18-3 5 µm 33 x 4.6 mm US2270C18-3 5 µm 50 x 4.6 mm US2280C18-3 5 µm 75 x 4.6 mm US2290





Octadecyl - USP code L1100Å - 425 m2/g ; %C : 19 mono functional + "multi step" end-capping


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RP 3 µm 20 x 2.0 mm US0530RP 3 µm 33 x 2.0 mm US0540RP 3 µm 50 x 2.0 mm US0550RP 5 µm 20 x 2.0 mm US0560RP 5 µm 33 x 2.0 mm US0570RP 5 µm 50 x 2.0 mm US0070RP 3 µm 50 x 3.0 mm US0510RP 5 µm 50 x 3.0 mm US0520RP 3 µm 33 x 4.0 mm US0490RP 5 µm 33 x 4.0 mm US0500RP 3 µm 20 x 4.6 mm US0420RP 3 µm 33 x 4.6 mm US0430RP 3 µm 50 x 4.6 mm US0440RP 3 µm 75 x 4.6 mm US0450RP 5 µm 20 x 4.6 mm US0460RP 5 µm 33 x 4.6 mm US0470RP 5 µm 50 x 4.6 mm US0030RP 5 µm 75 x 4.6 mm US0480 C8-2 3 µm 20 x 2.0 mm US2920C8-2 3 µm 33 x 2.0 mm US2930C8-2 3 µm 50 x 2.0 mm US2940C8-2 5 µm 20 x 2.0 mm US2950C8-2 5 µm 33 x 2.0 mm US2960C8-2 5 µm 50 x 2.0 mm US2970C8-2 3 µm 50 x 3.0 mm US2900C8-2 5 µm 50 x 3.0 mm US2910C8-2 3 µm 33 x 4.0 mm US2880C8-2 5 µm 33 x 4.0 mm US2890C8-2 3 µm 20 x 4.6 mm US2800C8-2 3 µm 33 x 4.6 mm US2810C8-2 3 µm 50 x 4.6 mm US2820C8-2 3 µm 75 x 4.6 mm US2830C8-2 5 µm 20 x 4.6 mm US2840C8-2 5 µm 33 x 4.6 mm US2850C8-2 5 µm 50 x 4.6 mm US2860C8-2 5 µm 75 x 4.6 mm US2870




Octyl - USP code L7100Å - 425 m2/g%C : 14mono functional + "one step" end-capping

Selectivity 13 cis/b-carotene all trans : 1Selectivity b-carotene/Zeaxanthine : 8.3Hydrophobicity b-carotene all trans : 23.66


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ODB 3 µm 20 x 2.0 mm UP3ODB#2HSODB 3 µm 33 x 2.0 mm UP3ODB#3HSODB 3 µm 50 x 2.0 mm UP3ODB#5HSODB 5 µm 33 x 2.0 mm UP5ODB#3HSODB 5 µm 50 x 2.0 mm UP5ODB#5HSODB 3 µm 50 x 3.0 mm UP3ODB$5HSODB 5 µm 50 x 3.0 mm UP5ODB$5HSODB 3 µm 33 x 4.0 mm UP3ODB*3HSODB 5 µm 33 x 4.0 mm UP5ODB*3HSODB 3 µm 20 x 4.6 mm UP3ODB-2HSODB 3 µm 33 x 4.6 mm UP3ODB-3HSODB 3 µm 50 x 4.6 mm UP3ODB-5HSODB 3 µm 75 x 4.6 mm UP3ODB-7HSODB 5 µm 33 x 4.6 mm UP5ODB-3HSODB 5 µm 50 x 4.6 mm UP5ODB-5HSODB 5 µm 75 x 4.6 mm UP5ODB-7HS HDO 3 µm 20 x 2.0 mm UP3HDO#2HSHDO 3 µm 33 x 2.0 mm UP3HDO#3HSHDO 3 µm 50 x 2.0 mm UP3HDO#5HSHDO 5 µm 33 x 2.0 mm UP5HDO#3HSHDO 5 µm 50 x 2.0 mm UP5HDO#5HSHDO 3 µm 50 x 3.0 mm UP3HDO$5HSHDO 5 µm 50 x 3.0 mm UP5HDO$5HSHDO 3 µm 33 x 4.0 mm UP3HDO*3HSHDO 5 µm 33 x 4.0 mm UP5HDO*3HSHDO 3 µm 20 x 4.6 mm UP3HDO-2HSHDO 3 µm 33 x 4.6 mm UP3HDO-3HSHDO 3 µm 50 x 4.6 mm UP3HDO-5HSHDO 3 µm 75 x 4.6 mm UP3HDO-7HSHDO 5 µm 33 x 4.6 mm UP5HDO-3HSHDO 5 µm 50 x 4.6 mm UP5HDO-5HSHDO 5 µm 75 x 4.6 mm UP5HDO-7HS


Uptisphere®

Octadecyl - USP code L1120Å - 320 m2/g%C : 17mono functional + + "mixed" end-capping





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NEC 3 µm 20 x 2.0 mm UP3NEC#2HSNEC 3 µm 33 x 2.0 mm UP3NEC#3HSNEC 3 µm 50 x 2.0 mm UP3NEC#5HSNEC 5 µm 33 x 2.0 mm UP5NEC#3HSNEC 5 µm 50 x 2.0 mm UP5NEC#5HSNEC 3 µm 50 x 3.0 mm UP3NEC$5HSNEC 5 µm 50 x 3.0 mm UP5NEC$5HSNEC 3 µm 33 x 4.0 mm UP3NEC*3HSNEC 5 µm 33 x 4.0 mm UP5NEC*3HSNEC 3 µm 20 x 4.6 mm UP3NEC-2HSNEC 3 µm 33 x 4.6 mm UP3NEC-3HSNEC 3 µm 50 x 4.6 mm UP3NEC-5HSNEC 3 µm 75 x 4.6 mm UP3NEC-7HSNEC 5 µm 33 x 4.6 mm UP5NEC-3HSNEC 5 µm 50 x 4.6 mm UP5NEC-5HSNEC 5 µm 75 x 4.6 mm UP5NEC-7HS HSC 3 µm 20 x 2.0 mm UP3HSC#2HSHSC 3 µm 33 x 2.0 mm UP3HSC#3HSHSC 3 µm 50 x 2.0 mm UP3HSC#5HSHSC 5 µm 33 x 2.0 mm UP5HSC#3HSHSC 5 µm 50 x 2.0 mm UP5HSC#5HSHSC 3 µm 50 x 3.0 mm UP3HSC$5HSHSC 5 µm 50 x 3.0 mm UP5HSC$5HSHSC 3 µm 33 x 4.0 mm UP3HSC*3HSHSC 5 µm 33 x 4.0 mm UP5HSC*3HSHSC 3 µm 20 x 4.6 mm UP3HSC-2HSHSC 3 µm 33 x 4.6 mm UP3HSC-3HSHSC 3 µm 50 x 4.6 mm UP3HSC-5HSHSC 3 µm 75 x 4.6 mm UP3HSC-7HSHSC 5 µm 33 x 4.6 mm UP5HSC-3HSHSC 5 µm 50 x 4.6 mm UP5HSC-5HSHSC 5 µm 75 x 4.6 mm UP5HSC-7HS

TF 5 µm 33 x 2.0 mm UP5TF#3HSTF 5 µm 50 x 2.0 mm UP5TF#5HSTF 5 µm 50 x 3.0 mm UP5TF$5HSTF 5 µm 33 x 4.0 mm UP5TF*3HSTF 5 µm 33 x 4.6 mm UP5TF-3HSTF 5 µm 50 x 4.6 mm UP5TF-5HSTF 5 µm 75 x 4.6 mm UP5TF-7HS


Uptisphere®








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C8 3 µm 20 x 2.0 mm UP3C8#2HSC8 3 µm 33 x 2.0 mm UP3C8#3HSC8 3 µm 50 x 2.0 mm UP3C8#5HSC8 5 µm 33 x 2.0 mm UP5C8#3HSC8 5 µm 50 x 2.0 mm UP5C8#5HSC8 3 µm 50 x 3.0 mm UP3C8$5HSC8 5 µm 50 x 3.0 mm UP5C8$5HSC8 3 µm 33 x 4.0 mm UP3C8*3HSC8 5 µm 33 x 4.0 mm UP5C8*3HSC8 3 µm 20 x 4.6 mm UP3C8-2HSC8 3 µm 33 x 4.6 mm UP3C8-3HSC8 3 µm 50 x 4.6 mm UP3C8-5HSC8 3 µm 75 x 4.6 mm UP3C8-7HSC8 5 µm 33 x 4.6 mm UP5C8-3HSC8 5 µm 50 x 4.6 mm UP5C8-5HSC8 5 µm 75 x 4.6 mm UP5C8-7HS C4 3 µm 20 x 2.0 mm UP3C4#2HSC4 3 µm 33 x 2.0 mm UP3C4#3HSC4 3 µm 50 x 2.0 mm UP3C4#5HSC4 5 µm 33 x 2.0 mm UP5C4#3HSC4 5 µm 50 x 2.0 mm UP5C4#5HSC4 3 µm 50 x 3.0 mm UP3C4$5HSC4 5 µm 50 x 3.0 mm UP5C4$5HSC4 3 µm 33 x 4.0 mm UP3C4*3HSC4 5 µm 33 x 4.0 mm UP5C4*3HSC4 3 µm 20 x 4.6 mm UP3C4-2HSC4 3 µm 33 x 4.6 mm UP3C4-3HSC4 3 µm 50 x 4.6 mm UP3C4-5HSC4 3 µm 75 x 4.6 mm UP3C4-7HSC4 5 µm 33 x 4.6 mm UP5C4-3HSC4 5 µm 50 x 4.6 mm UP5C4-5HSC4 5 µm 75 x 4.6 mm UP5C4-7HS CN 3 µm 20 x 2.0 mm UP3CN#2HSCN 3 µm 33 x 2.0 mm UP3CN#3HSCN 3 µm 50 x 2.0 mm UP3CN#5HSCN 5 µm 33 x 2.0 mm UP5CN#3HSCN 5 µm 50 x 2.0 mm UP5CN#5HSCN 3 µm 50 x 3.0 mm UP3CN$5HSCN 5 µm 50 x 3.0 mm UP5CN$5HSCN 3 µm 33 x 4.0 mm UP3CN*3HSCN 5 µm 33 x 4.0 mm UP5CN*3HSCN 3 µm 20 x 4.6 mm UP3CN-2HSCN 3 µm 33 x 4.6 mm UP3CN-3HSCN 3 µm 50 x 4.6 mm UP3CN-5HSCN 3 µm 75 x 4.6 mm UP3CN-7HSCN 5 µm 33 x 4.6 mm UP5CN-3HSCN 5 µm 50 x 4.6 mm UP5CN-5HSCN 5 µm 75 x 4.6 mm UP5CN-7HS


Uptisphere®

Butyl - USP code L26120Å - 320 m2/g%C : 7mono functional + "one step" end-capping

Cyano - USP code L10120Å - 320 m2/g%C : 8mono functional + "one step" end-capping




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Analysis - HPLC - Interchim technologyUptisphere® StrategyTM 1.7 - 2.2 µm & XS columns

HT/HR - High throughput, rapid analysis and high resolution HPLC market trends demand analytical tools that can achieve a high throughput of sample within ever reducing time frames. Interchim's 1.7 & 2.2 µm Uptisphere® StrategyTM stationary phases facilitate faster analyses, with high efficiency, whilst retaining sample resolution. They achieve unique separation and performance benefits through a combination of phase features, Interchim's proprietary packing processes, and the specifically designed Modulo-Cart XS hardware.

Interchim’s new 1.7 and 2.2 micron StrategyTM stationary phase. StrategyTM stationary phase has been developed upon 100 Å, 425m2/g & 450m2/g Upti-prepTM silica technology and is suitable for a diverse range of polar, mid-polar and non-polar compounds within the pH scale 1 –to- 10. A large quantity of accessible silanol groups are available for bonding due to Upti-prep's perfectly hydroxylated surface & cylindrical pores maximizing the loading capacity. The low surface energy ensures a perfect peak symmetry for basic compounds. The resultant Uptisphere® Strategy™ displays a higher loading capacity and excellent stability under aggressive mobile phase conditions such as basic buffers (pH: 1 to 10).

Uptisphere® Strategy™ 1.7 and 2.2 µm columns push the boundaries even further for high throughput of sample, maintaining high efficiencies and providing significant reductions in back pressure due to excellent permeability.

Modulo-cart XS

Modulo Cart XS Hardware

The Modulo-Cart XS hardware is an innovative, all-metal column that is extremely resistant to tearing and stable up to 3500 bars. Stringent manufacturing processes ensure that the high pressure demands, typically placed upon a 2 micron stationary phase, are comfortably managed.

Modulo-Cart XS is available in 2.1, 3.0 and 4.6 mm i.d and lengths of 20, 25, 30, 50, 100 and 150 mm. Product details are found on subsequent pages.

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Chromatographic considerationRetaining the same column length whilst moving to a smaller particle size provides an increase in sample resolution, working flow rate, the chromatographic cycle and decreases runtime. A reduction in column length provides the additional benefit of extensive savings in solvent usage.

Direct transposition with same resolutionSeparation of two compounds with a resolution of 2.0 on a 5µm, 250 x 4.6 column at a flow 1.0 ml /min

Particle size Dimension Ň/column Flow rate ml/min Retention time min Rs

5µm 250 x 4.6 mm 20 000 1.0 20 2.003µm 150 x 4.6 mm 18 500 1.0 12.6 1.90

2.2µm 100 x 4.6 mm 15 500 1.0 8 1.742.2µm 100 x 4.6 mm 16 500 2.0 4 1.80

1.7µm 100 x 4.6 mm 18 500 1.0 8 1.901.7µm 100 x 4.6 mm 20 000 2.5 3.2 1.97

1.7µm 50 x 4.6 mm 9250 1.0 4 1.341.7µm 50 x 4.6 mm 10 000 2.5 1.6 1.40

Particle size Dimension Flow rate ml/min Estimated cost

5µm 250 x 4.6 mm 1.0 1003µm 150 x 4.6 mm 1.0 64

2.2µm 100 x 4.6 mm 1.0 422.2µm 100 x 4.6 mm 2.0 24

1.7µm 100 x 4.6 mm 1.0 421.7µm 100 x 4.6 mm 2.5 21

1.7µm 50 x 4.6 mm 1.0 231.7µm 50 x 4.6 mm 2.5 6

Principle advantages of using 1.7 & 2.2 µm columns

Economic considerationThe table below highlights the significant economic benefit when comparing sample analysis using a classical 5µm column compared to a 2µm column. The evaluation considers the analysis of 1000 samples and the calculation is based upon an indice 100 for a 5µm, 250 x 4.6 mm column including column cost, solvent, accessories, transposition and labor. The study shows a 15 fold financial saving and the runtime of the separation drops down a factor of 10 and thus the dramatic gains both financially and in terms of productivity are self evident.

Separation of two compounds with a resolution of 2.0 on a 5µm, 250 x 4.6 column at a flow 1.0 ml /min


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Unrivalled quality

Uptisphere®StrategyTM 1.7 µm C18-2

Conditions : ACN /H2O (70/30)0.2 ml /minUV : 254 nm - 22°C

25 x 2.0 mmP/N : US5040

50 x 2.0 mmP/N : US3170

100 x 2.0 mmP/N : US3180

150 x 2.0 mmP/N : US3190

Pressure : 72 bars

FluoreneTr : 1.28 minAs : 1.28Plates : 4734

Pressure : 124 bars


Pressure : 221 bars


Pressure : 307 bars


25 x 2.0 mmP/N : US5060

50 x 2.0 mmP/N : US3060

100 x 2.0 mmP/N : US3070

150 x 2.0 mmP/N : US4900

Pressure : 42 bars


Pressure : 66 bars


Pressure : 157 bars


Pressure : 117 bars


Uptisphere®StrategyTM 2.2 µm C18-2

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Unrivalled quality

Uptisphere® StrategyTM 1.7 µm, 2.2 µm and 5 µm display perfect harmony in selectivity irrespective of column length or i.d.

Conditions : ACN /H2O (70/30) 0.2 ml /min - UV : 254 nm - 22°C

Uracil Toluene Fluorene K'1 K'2 α

25 x 2.1 mm 1.7 µm 0.23 0.74 1.28 2.22 4.57 0.49 2.2 µm 0.21 0.74 1.32 2.52 5.29 0.48

50 x 2.1 mm 1.7 µm 0.46 1.47 2.69 2.20 4.85 0.45 2.2 µm 0.43 1.49 2.65 2.47 5.16 0.48

100 x 2.1 mm 1.7 µm 0.96 3.04 5.4 2.17 4.63 0.47 2.2 µm 0.9 3.06 5.48 2.40 5.09 0.47

150 x 2.1 mm 1.7 µm 1.53 4.72 8.38 2.08 4.48 0.46 2.2 µm 1.42 4.7 8.39 2.31 4.91 0.47

100 x 2.0 mm

1.7 µm

2.2 µm


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Efficiency and pressure relative to flow ratefor a 50 x 2.0 mm column

Uptisphere® StrategyTM 1.7 µm and 2.2 µm exhibit high permeability that allow higher flow rates compared to competitor columns utilizing similar particle sizes. High pressure benefits are retained and therefore analysis can be performed at high speed without compromising the separation. E.g. up to 215 000 plates /m at 0.6 ml /min for a 1.7 µm. Phase permeability and innovate packing processes establishes the additional benefit of an extensive column lifetime.

Flow rate ml/min Pressure bars Symmetry Efficiency

StrategyTM 1.7 µm C18-2 0.2 121 1.18 8937US3170 0.3 172 1.18 10203 0.4 204 1.20 10272 0.5 214 1.19 10396 0.6 331 1.21 10754 0.7 390 1.25 10257 0.8 430 1.28 9697 0.9 450 1.37 8840 1 510

ACN - H2O (70/30) - UV : 254 nm - 22 C - compound : Fluorene

Flow rate ml/min Pressure bars Symmetry Efficiency

StrategyTM 2.2 µm C18-2 0.2 66 1.23 6570US3060 0.3 97 1.2 6869 0.4 124 1.2 6986 0.5 154 1.2 7115 0.6 180 1.2 7006 0.7 200 1.15 6215 0.8 217 1.17 7025 0.9 262 1.12 5697 1 293 1.09 5173

Lifetime QC of an Uptisphere® StrategyTM C18-2 at 720 bars

0

20000

40000

60000

80000

100000

120000

140000

160000

180000

0 1000 2000 3000 4000 5000 6000

A- purge : MeOH/H2O (80/20) at 720 barsB- control of Fluorene efficiency under ACN/H2O ( 70/30) 1,0 ml/min

Efficiency

Column volume

Maximum efficiency depends upon the nature of organic solvent within the mobile phase. i.e. Uptisphere® Strategy™ 2.2 µm C18-2 - maximum efficiency is obtained between 0.5 to 2 ml/min with Methanol and 1.75 to 3 ml/min using Acetonitrile. Temperature increases improve the mass transfer and should be considered for a given analysis to enhance separation whilst decreasing back pressure.

Care should be taken with optimization of the chromatography instrument to maximize benefit. This is particularly relevant with rapid analysis utilizing smaller stationary phase particles such as the Uptisphere® Strategy™ 1.7 and 2.2 µm columns. Tubing, detector acquisition, injection and pump must be carefully checked to adhere to the demands of small particle technology.

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Analysis - HPLC - Interchim technologyUptisphere® StrategyTM 1.7 & 2.2 µm & XS columns

Phase Particle size Dimension Modulo-Cart XS

C18-2 1.7 µm 20 x 4.6 mm US3080C18-2 1.7 µm 25 x 4.6 mm US5050C18-2 1.7 µm 30 x 4.6 mm US3090C18-2 1.7 µm 50 x 4.6 mm US3100C18-2 1.7 µm 100 x 4.6 mm US3110C18-2 1.7 µm 150 x 4.6 mm US3120C18-2 1.7 µm 50 x 3.0 mm US3130C18-2 1.7 µm 100 x 3.0 mm US3140C18-2 1.7 µm 20 x 2.0 mm US3150C18-2 1.7 µm 25 x 2.0 mm US5040C18-2 1.7 µm 30 x 2.0 mm US3160C18-2 1.7 µm 50 x 2.0 mm US3170C18-2 1.7 µm 100 x 2.0 mm US3180C18-2 1.7 µm 150 x 2.0 mm US3190C18-2 2.2 µm 20 x 4.6 mm US2980C18-2 2.2 µm 25 x 4.6 mm US5070C18-2 2.2 µm 30 x 4.6 mm US2990C18-2 2.2 µm 50 x 4.6 mm US3000C18-2 2.2 µm 100 x 4.6 mm US3010C18-2 2.2 µm 150 x 4.6 mm US5150C18-2 2.2 µm 50 x 3.0 mm US3020C18-2 2.2 µm 100 x 3.0 mm US3030C18-2 2.2 µm 20 x 2.0 mm US3040C18-2 2.2 µm 25 x 2.0 mm US5060C18-2 2.2 µm 30 x 2.0 mm US3050C18-2 2.2 µm 50 x 2.0 mm US3060C18-2 2.2 µm 100 x 2.0 mm US3070C18-2 2.2 µm 150 x 2.0 mm US4900 C18-HT 2.2 µm 20 x 4.6 mm US4800C18-HT 2.2 µm 25 x 4.6 mm US5090C18-HT 2.2 µm 30 x 4.6 mm US4810C18-HT 2.2 µm 50 x 4.6 mm US4820C18-HT 2.2 µm 100 x 4.6 mm US4830C18-HT 2.2 µm 150 x 4.6 mm US5290C18-HT 2.2 µm 50 x 3.0 mm US4780C18-HT 2.2 µm 100 x 3.0 mm US4790C18-HT 2.2 µm 20 x 2.0 mm US4740C18-HT 2.2 µm 25 x 2.0 mm US5080C18-HT 2.2 µm 30 x 2.0 mm US4750C18-HT 2.2 µm 50 x 2.0 mm US4760C18-HT 2.2 µm 100 x 2.0 mm US4770C18-HT 2.2 µm 150 x 2.0 mm US5300





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HILIC SI 2.2 µm 20 x 4.6 mm US5590HILIC SI 2.2 µm 25 x 4.6 mm US5600HILIC SI 2.2 µm 30 x 4.6 mm US5610HILIC SI 2.2 µm 50 x 4.6 mm US5620HILIC SI 2.2 µm 100 x 4.6 mm US5630HILIC SI 2.2 µm 150 x 4.6 mm US5640HILIC SI 2.2 µm 50 x 3.0 mm US5650HILIC SI 2.2 µm 100 x 3.0 mm US5660HILIC SI 2.2 µm 20 x 2.0 mm US5670HILIC SI 2.2 µm 25 x 2.0 mm US5680HILIC SI 2.2 µm 30 x 2.0 mm US5690HILIC SI 2.2 µm 50 x 2.0 mm US5700HILIC SI 2.2 µm 100 x 2.0 mm US5710HILIC SI 2.2 µm 150 x 2.0 mm US5720 SI 2.2 µm 20 x 4.6 mm US5160SI 2.2 µm 25 x 4.6 mm US5170SI 2.2 µm 30 x 4.6 mm US5180SI 2.2 µm 50 x 4.6 mm US5190SI 2.2 µm 100 x 4.6 mm US5200SI 2.2 µm 150 x 4.6 mm US5210SI 2.2 µm 50 x 3.0 mm US5220SI 2.2 µm 100 x 3.0 mm US5230SI 2.2 µm 20 x 2.0 mm US5240SI 2.2 µm 25 x 2.0 mm US5250SI 2.2 µm 30 x 2.0 mm US5260SI 2.2 µm 50 x 2.0 mm US5270SI 2.2 µm 100 x 2.0 mm US4960SI 2.2 µm 150 x 2.0 mm US5280




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Analysis - HPLC - Interchim technologyUptisphere® StrategyTM 2.2 µm for purification


C18-2 2.2 µm 50 x 10.0 mm US5870C18-2 2.2 µm 50 x 21.2 mm US5860

SI 2.2 µm 50 x 10.0 mm US5890SI 2.2 µm 50 x 21.2 mm US5880



2.2 µm for prep LC and LC/MS prepThe combination of Upti-prepTM silica technology with Interchim's silane synthesis process ensures a perfect particle size continuity and facilitates effortless chromatographic transposition from analytical development to prep LC or LC/MS purification.

LC/MS Prep application

Uracil Toluene Fluorene K'1 K'2 α

50 x 2.0 mm 1.7 µm 0.46 1.47 2.69 2.2 4.85 0.45 2.2 µm 0.43 1.49 2.65 2.47 5.16 0.48

50 x 10.0 mm 2.2 µm 0.41 1.41 2.54 2.44 5.19 0.47


ACN /H2O (70/30) UV : 254 nm - 22°C50 x 2.0 mm : 0.2 ml /min - P : 66 bars50 x 10.0 mm : 5 ml /min - P : 69 bars

50 x 10.0 mm50 x 2.0 mm


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Analysis - HPLC - Interchim technologyUptisphere® stationary phase & MC columns

1.0 mm internal diameter for high sensitivity

The Modulo-Cart MC range is a state-of the-art engineered 1.0 mm internal diameter hardware providing optimal sensitivity. A 20-fold increase in sensitivity has been de-monstrated in Interchim's laboratories in comparison to a 4.6 mm i.d. column. Modulo-Cart MC has proven value for trace analysis, LC/MS & LC/NMR applications, and in the bio-analytical market where there is limited availability of sample volume.

working flow range : 20 µl to 60 µl/mn. Optimal : 40 µl/mn maximum volume of injection is approximately 2 µl for a maximum sample mass of approximately 50 µg (fion k' of compounds) working flow range : 20 µl to 60 µl/mn. Optimal : 40 µl/mn MC available column length : 20, 50,100 &150 mm

Modulo-Cart MCK Delivered with a pre-installed guard cartridge of 1 mm length that is packed with the statio-nary phase of your own requirement.

Replacement guard cartridges are available i.e. Q95960, pack of 10 Q95961, pack of 3

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Phase Particle size Dimension Modulo-Cart MC Modulo-Cart MCK

C18-2 3 µm 20 x 1.0 mm US3670 C18-2 3 µm 50 x 1.0 mm US3680 US4010C18-2 3 µm 150 x 1.0 mm US3690 US4020C18-2 5 µm 50 x 1.0 mm US3700 US4030C18-2 5 µm 150 x 1.0 mm US3710 US4040 C18-3 3 µm 20 x 1.0 mm US3720 C18-3 3 µm 50 x 1.0 mm US3730 US4050C18-3 3 µm 150 x 1.0 mm US3740 US4060C18-3 5 µm 50 x 1.0 mm US3750 US4070C18-3 5 µm 150 x 1.0 mm US3760 US4080


ODB 3 µm 20 x 1.0 mm UP3ODBD2MC ODB 3 µm 50 x 1.0 mm UP3ODBD5MC UP3ODBD5MCKODB 3 µm 150 x 1.0 mm UP3ODBD15MC UP3ODBD15MCKODB 5 µm 20 x 1.0 mm UP5ODBD2MC ODB 5 µm 50 x 1.0 mm UP5ODBD5MC UP5ODBD5MCKODB 5 µm 150 x 1.0 mm UP5ODBD15MC UP5ODBD15MCK HDO 3 µm 20 x 1.0 mm UP3HDOD2MC HDO 3 µm 50 x 1.0 mm UP3HDOD5MC UP3HDOD5MCKHDO 3 µm 150 x 1.0 mm UP3HDOD15MC UP3HDOD15MCKHDO 5 µm 20 x 1.0 mm UP5HDOD2MC HDO 5 µm 50 x 1.0 mm UP5HDOD5MC UP5HDOD5MCKHDO 5 µm 150 x 1.0 mm UP5HDOD15MC UP5HDOD15MCK NEC 3 µm 20 x 1.0 mm UP3NECD2MC NEC 3 µm 50 x 1.0 mm UP3NECD5MC UP3NECD5MCKNEC 3 µm 150 x 1.0 mm UP3NECD15MC UP3NECD15MCKNEC 5 µm 20 x 1.0 mm UP5NECD2MC NEC 5 µm 50 x 1.0 mm UP5NECD5MC UP5NECD5MCKNEC 5 µm 150 x 1.0 mm UP5NECD15MC UP5NECD15MCK TF 5 µm 20 x 1.0 mm UP5TFD2MC TF 5 µm 50 x 1.0 mm UP5TFD5MC UP5TFD5MCKTF 5 µm 150 x 1.0 mm UP5TFD15MC UP5TFD15MCK C8 3 µm 20 x 1.0 mm UP3C8D2MC C8 3 µm 50 x 1.0 mm UP3C8D5MC UP3C8D5MCKC8 3 µm 150 x 1.0 mm UP3C8D15MC UP3C8D15MCKC8 5 µm 20 x 1.0 mm UP5C8D2MC C8 5 µm 50 x 1.0 mm UP5C8D5MC UP5C8D5MCKC8 5 µm 150 x 1.0 mm UP5C8D15MC UP5C8D15MCK


Octadecyl - USP code L1100Å - 425 m2/g%C : 22mono functional + "multi-step" end-capping


Uptisphere®



Octadecyl - USP code L1%C : 14poly functional + "one-step" end-capping

Octyl - USP code L7120Å - 320 m2/g%C : 11mono functional + "one-step" end-capping

Octadecyl - USP code L1120Å - 320 m2/g%C : 18mono functional + "one-step" end-capping



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Optimized columns for LC/MS applications

The evolution of packing technology facilitates development of specific protocols for a number of applications. Modulo-Cart MS has been optimized for LC/MS coupling, improving efficiency and sensitivity. Available in 100, 150 & 250 mm lengths with different i.d. For applications requiring shorter length columns the Modulo-Cart HS range, highlighted earlier in this section, should be considered.

Note :The choice of internal column diameter is relative to the MS interface and application.

Column protection with a guard cartridge requires the use of a specific guard cartridge holder. P/N : CH797540Guard cartridges are available in packs of 3 or 10. P/N are highlighted in the column protection section later in this chapter.

The LC/MS SurefitTM connector allows a quick and easy column connection to the MS interface. A 50 µm i.d. ensures a minimum dead volume. P/N : T80590

Interface Interface flow rate Optimal column flow rate

2.1 mm i.d. Electrospray 5 µl – 1 ml/min 0.2 ml/min3.0 mm i.d. Electrospray /APCI 5 µl – 1 ml/min 0.35 ml/min4.6 mm i.d. APCI 0.2 – 1 ml/min 0.8 ml/min

LC/MS Surefit™

non-polar polar

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Analysis - HPLC - Interchim technologyUptisphere® stationary phase & MS columns

Phase Particle size Dimension Modulo-Cart MS

C18-2 3 µm 100 x 2.0 mm US3300C18-2 3 µm 150 x 2.0 mm US3310C18-2 5 µm 100 x 2.0 mm US3320C18-2 5 µm 150 x 2.0 mm US3330C18-2 5 µm 250 x 2.0 mm US3340C18-2 3 µm 100 x 3.0 mm US3250C18-2 3 µm 150 x 3.0 mm US3260C18-2 5 µm 100 x 3.0 mm US3270C18-2 5 µm 150 x 3.0 mm US3280C18-2 5 µm 250 x 3.0 mm US3290C18-2 3 µm 100 x 4.6 mm US3200C18-2 3 µm 150 x 4.6 mm US3210C18-2 5 µm 100 x 4.6 mm US3220C18-2 5 µm 150 x 4.6 mm US3230C18-2 5 µm 250 x 4.6 mm US3240 C18-3 3 µm 100 x 2.0 mm US3450C18-3 3 µm 150 x 2.0 mm US3460C18-3 5 µm 100 x 2.0 mm US3470C18-3 5 µm 150 x 2.0 mm US3480C18-3 5 µm 250 x 2.0 mm US3490C18-3 3 µm 100 x 3.0 mm US3400C18-3 3 µm 150 x 3.0 mm US3410C18-3 5 µm 100 x 3.0 mm US3420C18-3 5 µm 150 x 3.0 mm US3430C18-3 5 µm 250 x 3.0 mm US3440C18-3 3 µm 100 x 4.6 mm US3350C18-3 3 µm 150 x 4.6 mm US3360C18-3 5 µm 100 x 4.6 mm US3370C18-3 5 µm 150 x 4.6 mm US3380C18-3 5 µm 250 x 4.6 mm US3390







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ODB 3 µm 100 x 4.6 mm UP3ODB-10MSODB 3 µm 150 x 4.6 mm UP3ODB-15MSODB 5 µm 100 x 4.6 mm UP5ODB-10MSODB 5 µm 150 x 4.6 mm UP5ODB-15MSODB 5 µm 250 x 4.6 mm UP5ODB-25MSODB 3 µm 100 x 3.0 mm UP3ODB$10MSODB 3 µm 150 x 3.0 mm UP3ODB$15MSODB 5 µm 100 x 3.0 mm UP5ODB$10MSODB 5 µm 150 x 3.0 mm UP5ODB$15MSODB 5 µm 250 x 3.0 mm UP5ODB$25MSODB 3 µm 100 x 2.0 mm UP3ODB#10MSODB 3 µm 150 x 2.0 mm UP3ODB#15MSODB 5 µm 100 x 2.0 mm UP5ODB#10MSODB 5 µm 150 x 2.0 mm UP5ODB#15MSODB 5 µm 250 x 2.0 mm UP5ODB#25MS HDO 3 µm 100 x 4.6 mm UP3HDO-10MSHDO 3 µm 150 x 4.6 mm UP3HDO-15MSHDO 5 µm 100 x 4.6 mm UP5HDO-10MSHDO 5 µm 150 x 4.6 mm UP5HDO-15MSHDO 5 µm 250 x 4.6 mm UP5HDO-25MSHDO 3 µm 100 x 3.0 mm UP3HDO$10MSHDO 3 µm 150 x 3.0 mm UP3HDO$15MSHDO 5 µm 100 x 3.0 mm UP5HDO$10MSHDO 5 µm 150 x 3.0 mm UP5HDO$15MSHDO 5 µm 250 x 3.0 mm UP5HDO$25MSHDO 3 µm 100 x 2.0 mm UP3HDO#10MSHDO 3 µm 150 x 2.0 mm UP3HDO#15MSHDO 5 µm 100 x 2.0 mm UP5HDO#10MSHDO 5 µm 150 x 2.0 mm UP5HDO#15MSHDO 5 µm 250 x 2.0 mm UP5HDO#25MS

NEC 3 µm 100 x 4.6 mm UP3NEC-10MSNEC 3 µm 150 x 4.6 mm UP3NEC-15MSNEC 5 µm 100 x 4.6 mm UP5NEC-10MSNEC 5 µm 150 x 4.6 mm UP5NEC-15MSNEC 5 µm 250 x 4.6 mm UP5NEC-25MSNEC 3 µm 100 x 3.0 mm UP3NEC$10MSNEC 3 µm 150 x 3.0 mm UP3NEC$15MSNEC 5 µm 100 x 3.0 mm UP5NEC$10MSNEC 5 µm 150 x 3.0 mm UP5NEC$15MSNEC 5 µm 250 x 3.0 mm UP5NEC$25MSNEC 3 µm 100 x 2.0 mm UP3NEC#10MSNEC 3 µm 150 x 2.0 mm UP3NEC#15MSNEC 5 µm 100 x 2.0 mm UP5NEC#10MSNEC 5 µm 150 x 2.0 mm UP5NEC#15MSNEC 5 µm 250 x 2.0 mm UP5NEC#25MS


Uptisphere®


Octadecyl - USP code L1120Å - 320 m2/g%C : 17mono functional + "mixed"end-capping



Selectivity 13 cis/b-carotene all trans : 1,0Selectivity b-carotene/Zeaxanthine : 8.81Hydrophobicity b-carotene all trans : 21.54


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TF 5 µm 100 x 4.6 mm UP5TF-10MSTF 5 µm 150 x 4.6 mm UP5TF-15MSTF 5 µm 250 x 4.6 mm UP5TF-25MSTF 5 µm 100 x 3.0 mm UP5TF$10MSTF 5 µm 150 x 3.0 mm UP5TF$15MSTF 5 µm 250 x 3.0 mm UP5TF$25MSTF 5 µm 100 x 2.0 mm UP5TF#10MSTF 5 µm 150 x 2.0 mm UP5TF#15MSTF 5 µm 250 x 2.0 mm UP5TF#25MS C8 3 µm 100 x 4.6 mm UP3C8-10MSC8 3 µm 150 x 4.6 mm UP3C8-15MSC8 5 µm 100 x 4.6 mm UP5C8-10MSC8 5 µm 150 x 4.6 mm UP5C8-15MSC8 5 µm 250 x 4.6 mm UP5C8-25MSC8 3 µm 100 x 3.0 mm UP3C8$10MSC8 3 µm 150 x 3.0 mm UP3C8$15MSC8 5 µm 100 x 3.0 mm UP5C8$10MSC8 5 µm 150 x 3.0 mm UP5C8$15MSC8 5 µm 250 x 3.0 mm UP5C8$25MSC8 3 µm 100 x 2.0 mm UP3C8#10MSC8 3 µm 150 x 2.0 mm UP3C8#15MSC8 5 µm 100 x 2.0 mm UP5C8#10MSC8 5 µm 150 x 2.0 mm UP5C8#15MSC8 5 µm 250 x 2.0 mm UP5C8#25MS


Uptisphere®


Selectivity 13 cis/b-carotene all trans : 1,205Selectivity b-carotene/Zeaxanthine : 2,5Hydrophobicity b-carotene all trans : 9



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Analysis - HPLC - Interchim technologyUptisphere® stationary phase & nano LC columns

Optimal sensitivity through novel hardware solutionsThe Modulo-Cart Nano LC is a state-of the-art engineered column hardware with a 250 µm & 500 µm internal diameter providing optimal sensitivity. A 50-100 fold increase in sensitivity has been demonstrated in Interchim's laboratories in comparison to a 4.6 mm i.d. column.

Modulo-Cart Nano has proven value for trace analysis and in the bio-analytical market where there is limited availability of valuable sample.

State-of-the art column engineering Frit minimizes sample interactions and hold up volume. Internal diameters 250 µm & 500 µm LC Nano available column length : 50,100 &150 mm

i.d. Flow rate range Optimal flow rate loading capacity

500 µm 4 to 12 µl 9 µl 0.1 µg250 µm 1.2 to 3 µl 2.2 µl 0.001 µg

Modulo-cart Nano LC

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Phase Particle size Dimension Modulo-Cart Nano

C18-2 3 µm 50 x 0.25 mm US3830C18-2 3 µm 100 x 0.25 mm US3840C18-2 3 µm 150 x 0.25 mm US3850C18-2 5 µm 50 x 0.25 mm US3860C18-2 5 µm 100 x 0.25 mm US3870C18-2 5 µm 150 x 0.25 mm US3880C18-2 3 µm 50 x 0.5 mm US3770C18-2 3 µm 100 x 0.5 mm US3780C18-2 3 µm 150 x 0.5 mm US3790C18-2 5 µm 50 x 0.5 mm US3800C18-2 5 µm 100 x 0.5 mm US3810C18-2 5 µm 150 x 0.5 mm US3820 C18-3 3 µm 50 x 0.25 mm US3950C18-3 3 µm 100 x 0.25 mm US3960C18-3 3 µm 150 x 0.25 mm US3970C18-3 5 µm 50 x 0.25 mm US3980C18-3 5 µm 100 x 0.25 mm US3990C18-3 5 µm 150 x 0.25 mm US4000C18-3 3 µm 50 x 0.5 mm US3890C18-3 3 µm 100 x 0.5 mm US3900C18-3 3 µm 150 x 0.5 mm US3910C18-3 5 µm 50 x 0.5 mm US3920C18-3 5 µm 100 x 0.5 mm US3930C18-3 5 µm 150 x 0.5 mm US3940

Uptisphere® Strategy™Octadecyl - USP code L1100Å - 425 m2/g%C : 19mono functional + "multi-step" end-capping





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Phase Particle size Dimension Modulo-Cart Nano

ODB 3 µm 50 x 0.5 mm UP3ODBB5ODB 3 µm 100 x 0.5 mm UP3ODBB10ODB 3 µm 150 x 0.5 mm UP3ODBB15ODB 5 µm 50 x 0.5 mm UP5ODBB5ODB 5 µm 100 x 0.5 mm UP5ODBB10ODB 5 µm 150 x 0.5 mm UP5ODBB15ODB 3 µm 50 x 0.25 mm UP3ODBA5ODB 3 µm 100 x 0.25 mm UP3ODBA10ODB 3 µm 150 x 0.25 mm UP3ODBA15ODB 5 µm 50 x 0.25 mm UP5ODBA5ODB 5 µm 100 x 0.25 mm UP5ODBA10ODB 5 µm 150 x 0.25 mm UP5ODBA15 NEC 3 µm 50 x 0.5 mm UP3NECB5NEC 3 µm 100 x 0.5 mm UP3NECB10NEC 3 µm 150 x 0.5 mm UP3NECB15NEC 5 µm 50 x 0.5 mm UP5NECB5NEC 5 µm 100 x 0.5 mm UP5NECB10NEC 5 µm 150 x 0.5 mm UP5NECB15NEC 3 µm 50 x 0.25 mm UP3NECA5NEC 3 µm 100 x 0.25 mm UP3NECA10NEC 3 µm 150 x 0.25 mm UP3NECA15NEC 5 µm 50 x 0.25 mm UP5NECA5NEC 5 µm 100 x 0.25 mm UP5NECA10NEC 5 µm 150 x 0.25 mm UP5NECA15 TF 5 µm 50 x 0.5 mm UP5TFB5TF 5 µm 100 x 0.5 mm UP5TFB10TF 5 µm 150 x 0.5 mm UP5TFB15TF 5 µm 50 x 0.25 mm UP5TFA5TF 5 µm 100 x 0.25 mm UP5TFA10TF 5 µm 150 x 0.25 mm UP5TFA15


Uptisphere®







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Analysis - HPLC - Interchim technologyUptisphere® BioP I & BioP II stationary phase & columns

Optimized for bio analysis

Uptisphere® BioP I & BioP II are Interchim's proprietary stationary phases to serve bio-analysis /DMPK Lab requirement. They achieve excellent analysis for crude samples without the risk of contaminating the column head or clogging and subsequently maintain a longer column life. Based upon a C18 chemistry, BioP I and BioP II display similar separation selectivity to Uptisphere® ODB and HDO. Uptisphere® BioP I & BioP II are available in a range of Modulo-Cart hardware relative to separation requirements.

Phase Bonding Pore size Surface area % C end-capping USP code

Uptisphere BioP I C18 n.c. n.c. 18 one step L1Uptisphere BioP II C18 n.c. n.c. 17 mixed L1

Modulo-cart BioP

A sample preparation pre-step should be performed, if feasible, with an appropriate SPE sorbent, prior to HPLC injection.


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BP1 3 µm 20 x 1.0 mm UP3BP1D2MC BP1 3 µm 50 x 1.0 mm UP3BP1D5MC UP3BP1D5MCKBP1 3 µm 150 x 1.0 mm UP3BP1D15MC UP3BP1D15MCKBP1 5 µm 20 x 1.0 mm UP5BP1D2MC BP1 5 µm 50 x 1.0 mm UP5BP1D5MC UP5BP1D5MCKBP1 5 µm 150 x 1.0 mm UP5BP1D15MC UP5BP1D15MCK Phase Particle size Dimension Modulo-Cart HS

BP1 3 µm 20 x 4.6 mm UP3BP1-2HS BP1 3 µm 33 x 4.6 mm UP3BP1-3HS BP1 3 µm 50 x 4.6 mm UP3BP1-5HS BP1 3 µm 75 x 4.6 mm UP3BP1-7HS BP1 5 µm 20 x 4.6 mm UP5BP1-2HS BP1 5 µm 33 x 4.6 mm UP5BP1-3HS BP1 5 µm 50 x 4.6 mm UP5BP1-5HS BP1 5 µm 75 x 4.6 mm UP5BP1-7HS BP1 3 µm 33 x 4.0 mm UP3BP1*3HS BP1 5 µm 33 x 4.0 mm UP5BP1*3HS BP1 3 µm 50 x 3.0 mm UP3BP1$5HS BP1 5 µm 50 x 3.0 mm UP5BP1$5HS BP1 3 µm 20 x 2.0 mm UP3BP1#2HS BP1 3 µm 33 x 2.0 mm UP3BP1#3HS BP1 3 µm 50 x 2.0 mm UP3BP1#5HS BP1 5 µm 20 x 2.0 mm UP5BP1#2HS BP1 5 µm 33 x 2.0 mm UP5BP1#3HS BP1 5 µm 50 x 2.0 mm UP5BP1#5HS Phase Particle size Dimension Modulo-Cart QS Modulo-Cart QK

BP1 5 µm 100 x 2.0 mm UP5BP1#10QS UP5BP1#10QKBP1 5 µm 125 x 2.0 mm UP5BP1#12QS UP5BP1#12QKBP1 5 µm 150 x 2.0 mm UP5BP1#15QS UP5BP1#15QKBP1 5 µm 100 x 3.0 mm UP5BP1$10QS UP5BP1$10QKBP1 5 µm 125 x 3.0 mm UP5BP1$12QS UP5BP1$12QKBP1 5 µm 150 x 3.0 mm UP5BP1$15QS UP5BP1$15QKBP1 5 µm 100 x 4.0 mm UP5BP1*10QS UP5BP1*10QKBP1 5 µm 125 x 4.0 mm UP5BP1*12QS UP5BP1*12QKBP1 5 µm 150 x 4.0 mm UP5BP1*15QS UP5BP1*15QKBP1 5 µm 100 x 4.6 mm UP5BP1-10QS UP5BP1-10QKBP1 5 µm 125 x 4.6 mm UP5BP1-12QS UP5BP1-12QKBP1 5 µm 150 x 4.6 mm UP5BP1-15QS UP5BP1-15QK


Uptisphere®

Octadecyl - USP code L1%C : 18mono functional + "one step"end-capping


QS QK

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B.74



BP2 3 µm 20 x 1.0 mm UP3BP2D2MC BP2 3 µm 50 x 1.0 mm UP3BP2D5MC UP3BP2D5MCKBP2 3 µm 150 x 1.0 mm UP3BP2D15MC UP3BP2D15MCKBP2 5 µm 20 x 1.0 mm UP5BP2D2MC BP2 5 µm 50 x 1.0 mm UP5BP2D5MC UP5BP2D5MCKBP2 5 µm 150 x 1.0 mm UP5BP2D15MC UP5BP2D15MCK Phase Particle size Dimension Modulo-Cart HS

BP2 3 µm 20 x 4.6 mm UP3BP2-2HS BP2 3 µm 33 x 4.6 mm UP3BP2-3HS BP2 3 µm 50 x 4.6 mm UP3BP2-5HS BP2 3 µm 75 x 4.6 mm UP3BP2-7HS BP2 5 µm 20 x 4.6 mm UP5BP2-2HS BP2 5 µm 33 x 4.6 mm UP5BP2-3HS BP2 5 µm 50 x 4.6 mm UP5BP2-5HS BP2 5 µm 75 x 4.6 mm UP5BP2-7HS BP2 3 µm 33 x 4.0 mm UP3BP2*3HS BP2 5 µm 33 x 4.0 mm UP5BP2*3HS BP2 3 µm 50 x 3.0 mm UP3BP2$5HS BP2 5 µm 50 x 3.0 mm UP5BP2$5HS BP2 3 µm 20 x 2.0 mm UP3BP2#2HS BP2 3 µm 33 x 2.0 mm UP3BP2#3HS BP2 3 µm 50 x 2.0 mm UP3BP2#5HS BP2 5 µm 20 x 2.0 mm UP5BP2#2HS BP2 5 µm 33 x 2.0 mm UP5BP2#3HS BP2 5 µm 50 x 2.0 mm UP5BP2#5HS Phase Particle size Dimension Modulo-Cart QS Modulo-Cart QK

BP2 5 µm 100 x 2.0 mm UP5BP2#10QS UP5BP2#10QKBP2 5 µm 125 x 2.0 mm UP5BP2#12QS UP5BP2#12QKBP2 5 µm 150 x 2.0 mm UP5BP2#15QS UP5BP2#15QKBP2 5 µm 100 x 3.0 mm UP5BP2$10QS UP5BP2$10QKBP2 5 µm 125 x 3.0 mm UP5BP2$12QS UP5BP2$12QKBP2 5 µm 150 x 3.0 mm UP5BP2$15QS UP5BP2$15QKBP2 5 µm 100 x 4.0 mm UP5BP2*10QS UP5BP2*10QKBP2 5 µm 125 x 4.0 mm UP5BP2*12QS UP5BP2*12QKBP2 5 µm 150 x 4.0 mm UP5BP2*15QS UP5BP2*15QKBP2 5 µm 100 x 4.6 mm UP5BP2-10QS UP5BP2-10QKBP2 5 µm 125 x 4.6 mm UP5BP2-12QS UP5BP2-12QKBP2 5 µm 150 x 4.6 mm UP5BP2-15QS UP5BP2-15QK

Uptisphere®

Octadecyl%C : 17mono functional + "mixed" end-capping


QS QK


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B.75

Analysis - HPLC - Interchim technologyUpti-trapTM sample preparation columns

On-line extractionThe Modulo-cart Upti-trapTM provides an easy on-line extraction and /or sample pre concentration prior to HPLC analysis. It prevents clogging and potential damage to the analytical column. Easily automated, Upti-trapTM are available in two sizes.

Upti-trap has proven valuable as a pre-concentration tool for environmental samples (PAH analysis, PCB, phenyl-ureas, triazines, carbamate etc) and for biological fluid analysis.

2.Elution modeThe compounds of interest are eluted by the mobile phase via the second pump (mobile phase B) and passed into the HPLC column.

Name Dimension Loading CapacityUpti-trap 15 x 2.1mm 1.5 -to- 3mgUpti-trap 15 x 4.0mm 8 -to- 12mg

1. Extraction modeThe compounds of interest are trapped onto the sorbent while the residual sample is removed through the waste port with the washing solvent (mobile phase A). Two HPLC pumps are necessary, one for extraction the other for elution.

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A 10 port, 2 position valve simultaneously facilitates sample extraction and second sample analysis, improving productivity.

Adsorbant µm 15 x 4.0 mm 15 x 2.1 mm

Upti-clean™ C18 end-capped 50 U25750 U25840Upti-clean™ C8 end-capped 50 BG6090 CE0580Upti-clean™ RPAQ C18 100% hydrophilic 75 U25760 U25850Upti-clean™ NH2 50 BV3700 BV3720Upti-clean™ SCX strong cation exchanger 60 U25770 U25860Upti-clean™ SAX strong anion exchanger 60 BB8650 BG6950Upti-clean™ MM1 RP / strong cation exchanger 60 U25780 U25870 Atoll XC Xtrem Capacity 30 U70480 U70500Atoll ATH hydrophilic 30 CE0560 CE0590Atoll AEV hydrophilic /hydrophobic 75 U25820 U25910Atoll ATL hydrophobic 75 U25800 U25890Atoll XWP wide pore Xtrem Capacity 90 BU5550 BU5560


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Direct injection of complex matrix

The Uptisphere® RP.AQ stationary phase is a mixed hydrophilic /hydrophobic polarity phase that allows direct drug injection from biological matrices. A selection valve is used to initially remove the matrix. Drugs are directed either to the MS detector or, in the case of a drug mix, a C18-based analytical column prior to detection.

Phase Dimension P/N

Uptisphere® RP.AQ 50 x 1.0 mm CE9010Uptisphere® RP.AQ 50 x 2.1 mm CE9020

Chromatographic profile

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Analysis - HPLC - Interchim technologyUptisphere® stationary phase & Modulo-cart US columns

Compound identification and pre-purificationModulo-cart US is designed to facilitate rapid sample identification prior to the purification step. They are generally used as a control on purity for compounds that have just been synthesized. This format is particularly suited to the organic chemist working in drug discovery, medchem, chemical development and HTS services.

The Uptisphere® StrategyTM C18-2 and C18-3 phases provide optimal efficiency and high loading capacity. Interchim's proprietary packing processes optimize the number of samples that can be analyzed with high mobile phase flow rates.

Phase Particle size Dimension Modulo-Cart US

C18-2 3 µm 33 x 4.6 mm US3500C18-2 3 µm 50 x 4.6 mm US3510C18-2 3 µm 75 x 4.6 mm US3520C18-2 3 µm 100 x 4.6 mm US3530C18-2 5 µm 33 x 4.6 mm US3540C18-2 5 µm 50 x 4.6 mm US3560C18-2 5 µm 75 x 4.6 mm US3570C18-2 5 µm 100 x 4.6 mm US3580 C18-3 3 µm 33 x 4.6 mm US3590C18-3 3 µm 50 x 4.6 mm US3600C18-3 3 µm 75 x 4.6 mm US3610C18-3 3 µm 100 x 4.6 mm US3620C18-3 5 µm 33 x 4.6 mm US3630C18-3 5 µm 50 x 4.6 mm US3640C18-3 5 µm 75 x 4.6 mm US3650C18-3 5 µm 100 x 4.6 mm US3660





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B.79

Analysis - HPLC - Interchim technologyUptisphere® stationary phase & Modulo-cart US columns

Phase Particle size Dimension Modulo-CartUS

ODB 3 µm 33 x 4.6 mm UP3ODB-3USODB 3 µm 50 x 4.6 mm UP3ODB-5USODB 3 µm 75 x 4,6 mm UP3ODB-7USODB 3 µm 100 x 4.6 mm UP3ODB-10USODB 5 µm 33 x 4.6 mm UP5ODB-3USODB 5 µm 50 x 4.6 mm UP5ODB-5USODB 5 µm 75 x 4.6 mm UP5ODB-7USODB 5 µm 100 x 4.6 mm UP5ODB-10US NEC 3 µm 33 x 4.6 mm UP3NEC-3USNEC 3 µm 50 x 4.6 mm UP3NEC-5USNEC 3 µm 75 x 4.6 mm UP3NEC-7USNEC 3 µm 100 x 4.6 mm UP3NEC-10USNEC 5 µm 33 x 4.6 mm UP5NEC-3USNEC 5 µm 50 x 4.6 mm UP5NEC-5USNEC 5 µm 75 x 4.6 mm UP5NEC-7USNEC 5 µm 100 x 4.6 mm UP5NEC-10US TF 5 µm 33 x 4.6 mm UP5TF-3USTF 5 µm 50 x 4.6 mm UP5TF-5USTF 5 µm 75 x 4.6 mm UP5TF-7USTF 5 µm 100 x 4.6 mm UP5TF-10US C8 3 µm 33 x 4.6 mm UP3C8-3USC8 3 µm 50 x 4.6 mm UP3C8-5USC8 3 µm 75 x 4.6 mm UP3C8-7USC8 3 µm 100 x 4.6 mm UP3C8-10USC8 5 µm 33 x 4.6 mm UP5C8-3USC8 5 µm 50 x 4.6 mm UP5C8-5USC8 5 µm 75 x 4.6 mm UP5C8-7USC8 5 µm 100 x 4.6 mm UP5C8-10US

Uptisphere®


Octadecyl - USP code L1120Å - 320 m2/g%C : 16mono functional non end-capped


Octyl - USP code L7120Å - 320 m2/g%C : 11mono functional + "one-step" end-capping



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B.80

Analysis - HPLC - Interchim technologyUptisphere® stationary phase & Modulo-cart Prep columns

Purification capacity for a 250 mm length column relative to internal diameter Internal diameter Purification capacity*

4.6 mm 0.5 to 10 mg 10.0 mm 2 to 50 mg 21.2 mm 20 to 200 mg 28.0 mm 50 to 400 mg 50.8 mm 100 mg to 1 g

*Capacity depends on the ratio : peak of interest Resolution (Rs) /impurities to purify

Preparative HPLCInterchim Preparative columns range from 4.6 to 50.8 mm i.d and are for the purification of samples ranging from 0.5 mg to 1 gram. Preparative HPLC places specific demands on the components that form a preparative column. Interchim's Modulo-cart preparative columns pay particular attention to both the component and collective manufacturing processes.

Column tubing & column packing The tube polishing value (Ra) has a fundamental importance in preparative chromatography. A primary reason for broadening peaks and low efficiency is the utilization of a poorer quality tubing. Molecules in the center of the mobile phase stream can move more rapidly than the molecules closer to the side due to friction against the tubing sur-face. The lower the Ra value, the smoother the surface is, and the less "drag" the tubing will place upon a given separation. Modulo-cart preparative columns pay particular attention to this potential negative phenomenon. All columns have extremely smooth internal surfaces (typically 8 µ inch of Ra) to considerably reduce issues of drag and maintain column efficiency. Efficiency is also managed through Interchim's state-of-the art proprietary packing processes - Modulo-cart Prep withstand packing pressures up to 550 bars contributing strongly to a good bed stability and column life time.

Sample dispersionThe loading of sample onto a preparative column requires stringent management to establish quality separations. Column overloading results in a poor retention of pure fraction and therefore particular attention needs to be placed upon selecting the appropriate column dimension and the properties of the stationary phase. In addition, a careful control of the introduction of sample to the column is necessary to establish a homogeneous sample dispersion through the sorbent bead head. Sample typically enters a preparative column through a 1/16” fitting; poor sample loading will lead to overloading certain areas of the stationary phase whilst other areas will be underloaded.E.g. For a 50 mm i.d column with a 500 µm i.d capillary fitting - sample introduced to the column (without any sample distributor) will only interact with 0.01% of the surface column head. As well as a dramatic loss in capacity there will also be a high potential for the column head to prematurely clog, rapidly reducing column life times. To prevent this problem Interchim's Modulo-cart Preparative columns are outfitted with a sample distributor. The sample distributor design maximizes the efficiency of sample volume dispersion and the sample mass introduced to the surface of the column head.

Stationary phaseUpti-prep® technology not only provides extreme mechanical stablity for all Uptisphere® stationary phases, stability is also assured for all particle sizes for a given stationary phase. Interchim’s proprietary Uptisphere® ( 320 m2/g) and Uptisphere® StrategyTM (425 m2/g) stationary phases guarantee perfect batch to batch reproducibility and feature high loading capacities in a range of different selectivities. The seamless retention of quality from analytical phases with sub 2, 3 and 5 µm silica particles to preparative 10 and 15 µm particles place Interchim’s Modulo-cart preparative range at the forefront of preparative HPLC.


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Analysis - HPLC - Interchim technologyUptisphere® stationary phase & Modulo-cart Prep columns

Uptisphere® Strategy™ 100 ÅI.D. Length Particle size C18-3 C18-2 RP C8 Silica

10.0 mm 50 mm 5 µm US4120 US4270 US4350 US4470 US4620 250 mm 5 µm US4130 US1300 US4360 US4480 US4630 250 mm 10 µm US4140 US1770 US4370 US4490 US1760 21.2 mm 50 mm 5 µm US4150 US1180 US4380 US4500 US4640 250 mm 5 µm US4160 US1210 US4390 US4510 US4650 250 mm 10 µm US4170 US1190 US4400 US4520 US4660 250 mm 15 µm US4180 US4280 US4410 US4530 US4670 28.0 mm 50 mm 5 µm US4190 US4290 US4420 US4540 US4680 250 mm 5 µm US4210 US1270 US1780 US4550 US4690 250 mm 10 µm US4220 US4300 US1790 US4560 US4700 250 mm 15 µm US4230 US4310 US4430 US4570 US4710 50.8 mm 50 mm 5 µm 250 mm 10 µm US4250 US4330 US4450 US4600 US1350 250 mm 15 µm US4260 US4340 US4590 US4610 US4730

Uptisphere® 120 ÅI.D. Length Particle size ODB TF C8 SI

10.0 mm 50 mm 5 µm UP5ODB.5M UP5TF.5M UP5C8.5M UP5SI.5M 250 mm 5 µm UP5ODB.25M UP5TF.25M UP5C8.25M UP5SI.25M 250 mm 10 µm UP10ODB.25M UP10C8.25M UP10.25M 21.2 mm 50 mm 5 µm UP5ODB/5M UP5TF/5M UP5C8/5M UP5SI/5M 250 mm 5 µm UP5ODB/25M UP5TF/25M UP5C8/25M UP5SI/25M 250 mm 10 µm UP10ODB/25M UP10C8/25M UP10/25M 250 mm 15 µm UP15ODB/25M UP15C8/25M UP15/25M 28.0 mm 50 mm 5 µm UP5ODB&5M UP5TF&5M UP5C8&5M UP5SI&5M 250 mm 5 µm UP5ODB&25M UP5TF&25M UP5C8&25M UP5SI&25M 250 mm 10 µm UP10ODB&25M UP10C8&25M UP10&25M 250 mm 15 µm UP15ODB&25M UP15C8&25M UP15&25M 50.8 mm 50 mm 5 µm UP5ODB@5M UP5TF@5M UP5C8@5M UP5SI@5M 250 mm 10 µm UP10ODB@25M UP10C8@25M UP10@25M 250 mm 15 µm UP15ODB@25M UP15C8@25M UP15@25M

Interchim offer kits to provide a seamless transposition from the analytical to the preparative process.

Kits contain the analytical and prep column packed with the same batch of stationary phase .Kits are available upon request.

Analytical to Preparative transposition

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Analysis - HPLC - Interchim technologyApplications

Hydrocortisone butyrateUptisphere 120 Å, 3µ C18-HDO, 150 x 3.0 mm

ACN H2O (44/56)UV : 232 nm Courtesy of VDS Optilabs (Courtesy of VDS Optilabs)

1 - Hydrocortisone2 - Hydrocortisone 21-butyrat3 - Hydrocortisone 17-butyrat

1 - Hydrocortisone2 - Hydrocortisone 21-butyrat3 - Hydrocortisone 17-butyrat

UP3HDO$15QS UP3HDO$15QS

CatecholaminesUptisphere 120 Å, 3 µ C18-ODB, 125 x 3.9 mm

Catecholamines from CD1 Mouse Brain StructureUptisphere 120 Å ,3 µ C18-ODB, 150 x 4.6 mmStriatum + cortex + sea-horse

Catechol derivatives, Indol & SalicylateUptisphere 120 Å, 3 µ C18-ODB, 150 x 4.6 mmfrom Sprague-Dawley Rats Brains Dialysis

Guaiacol probe for ZeoliteUptisphere 120 Å, 3 µ C18-HDO, 150 x 3.0 mm

Flow rate : 0.60 ml/minInjection : 10 µl

(Na2HPO4 50 mmoles/I + EDTA 2.69 mmoles/I + Pic B7 1.25 mmoles/I), MeOH 13 % v/v - pH 3.95 Flow rate : 0.9 ml/min - Inj. 40 µl at 8°C - Column temperature : 24 °C - Electrochemical detection (E1 :100 mV - E2 : + 450 mV)

(Na2HPO4 50 mmoles/l + EDTA 2.69 mmoles/l + Pic B7 1.25 mmoles/l)MeOH 13% v/v - pH : 3.15 - Flow rate : 1 ml/min - Inj : 10 µl at 8 °CColumn temperature : 40 °C - Electrochemical detection (E1 : - 100mV ; E2 : + 180 mV)

MeOH H2O (80/20)Flow rate : 1 ml/minUV : 254 nm

1 - 2,2’-bipyridil2 - Guajakol3 - 2,3-dihydroxynaphthalene

6.04 Noradrenaline7.78 Adrenaline9.36 Internal Std.

5.13 3.4 DBHA (internal Std)6.56 DA8.30 DOPAC12.72 3-MT14.68 5-HIAA15.96 5-HT17.88 HVA

3.39 DOPA4.80 3,4 DHBA (Internal Std)6.44 DA7.53 2,5 DHBA8.60 DOPAC10.42 2,3 DHBA13.60 5-HIAA14.48 5-HT21.27 HVA


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BioanalysisUptisphere 120 Å, 3 µ C18-ODB, 30 x 2.0 mmPure product

Flow rate : 0,150 ml/minA : H2O + 0.1% Formic acid - B : Acetonitrile + 0.1% Formic acidGradient : 10% B in 1.5 min; 3 min a 10% B ; 0% phase B en 0.5 min2 min stabilisation - Run time : 6 min

Injection quantity : 30 µl266 > 152 4 ng ISTD278 > 152 0.04 ng

Detection : MS/MS Electrospray + CID Argon. Micromass QuattroLCZ

Injection quantity : 278 > 152 5 pg 266 > 152 4 ng

Injection 30 µl 266 > 152 4 ng ISTD 278 > 152 0.04 ng

Detection : MS/MS Electrospray+ CID Argon. Micromass QuattroLCZ

Uptisphere 120 Å, 3 µ C18-ODB, 30 x 2.0 mmLimit of Quantification

Uptisphere 120 Å, 3 µ C18-ODB, 30 x 2.0 mmExtract : 500 µl of plasma after SPE C18 extraction

(Courtesy of Dpt Bioanalyse /PRI /MAP Bristol-Myers Squibb Saint-Nazaire France)

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MalondialdehydeUptisphere 3 µ BioP 2, 100 x 2.0 mm

Anti-paludeen drugsUptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

NH4 15 mM, pH : 2 11 % Methanol 11 % ACNFlow rate : 190 µl /minTemp : 55°C Detection : MS

ACN 28%MeOH 20% - HClO4 30 mM - (pH : 4) 52% - 0,6 ml/min

1- Malondialdehyde2- IS Risperidone


Courtesy of Dr Steghens Biochimie C sce Pr Collombel HEH

1- Didesethylchloroquine2- Monodesethylchloroquine3- Chloroquine4- Chlorophenylquanil5- Cycloquanyl6- Roquanil

Syrup (Pharmaceutical + preservatives, without sugar)Uptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

83 % KH2PO4 0,05 M including 15 % heptane sulfonate Na17 % ACNFlow : 1.5 ml/minUV : 280 nm


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Basic DrugsUptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

Kawalactone from piper methysticum FORSTUptisphere 120 Å, 5 µ ODB, 250 x 3.9 mm

27% MeOH73% Acetate buffer pH : 5.73Flow rate : 1 ml/min

ACN 10% - Buffer (pH : 4,4) 90%1.5 ml/min - 45°C - UV 262 nm

HexaneDioxane1.5 ml/minUV 240 nm

5,03 Nicotine6,17 Tranhydroxycotinin10,84 Cotinin16,38 Caffeine

12,85 Desmethoxyyangonine16,55 Dihydrokawaine19,50 Yangonine23,06 Kawaine26,91 Dihydromethysticine38,03 Methysticine

(Courtesy of Pr Ch Bartomeuf phD Clermont-FD University)

Cotinine (urinary pool std 100µ/lUptisphere 120 Å 5 µ C18-ODB, 250 x 4.6 mm

Urine extract (cotinine 52 µ/l)Uptisphere 120 Å 5 µ ODB, 250 x 4.6 mm

ACN 10% - Buffer (pH : 4.4) 90%1.5 ml/min - 45°C - UV 262 nm

UP5ODB-25QS

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UV 295 nm

Flavors & FragrancesUptisphere 120 Å, 5 µ HDO, 150 x 3.9 mm


5.63 5-MeFurfural OH15.09 Me-5-Furfural16.95 Vanilic acid21.05 Syringic acid

Amine oxidesUptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

Organic AcidsUptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

N-Methylcarbamates in waterUptisphere 120 Å, 5 µ C18-HDO, 150 x 4.6 mm

MeOH 72% - ACN 9%H2O 19%1 ml/min40°C

H2O-ACN (95/5) + 0.1 % TFA1 ml/min120 barsUV 210

H2O-MeOH (t = 0 (80/20) ; t = 40 (45/55) ; t = 50 (0/100) during 5 min) 1 ml/minOPA derivatization : ex 330 nm ; em 466 nm

4.84 Aldicarb sulfoxide5.62 Aldicarb sulfon5.92 Oxamyl7.30 Methomyl14.60 Carbofuran 3-hydroxide20.86 Aldicarb28.24 Propoxur29.09 Carbofuran32.30 Carbaryl45.70 Methiocarb46.57 Internal std BDMC

1- Formic acid2- Acetic acid


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HerbicidesUptisphere 120 Å 5 µ ODB, 250 x 4.6 mm

UV 220 nm21,25 Mevinphos28,80 Dicamba31,06 Bromoxynil31,44 2.4-D34,46 MCPA34,90 2.4.5-T44,16 Coumaphos

UV 240 nm29,31 Chlorotoluron30,50 Isoproturon30,95 Diuron34,22 Ioxynil35,97 Linuron

UV 270 nm39,17 Diflubenzuron43,64 Dinoterbe

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20 µl - C : 175 mg/ml ofUptisphere 120 Å 3 µ C18-ODB, 50 x 4.6 mm

500 µl - C : 175 mg/ml ofUptisphere 120 Å, 3 µ C18-ODB, 100 x 10 mm

Oil cuts separation on DNAP (C17-C19 n-alcanes range)

Gradient ACN-H2O1 ml/minUVRed trace 254 nm

Gradient ACN-H2O1 ml/minUVBlue trace 220 nm Red trace 254 nm

7-10 min : monoaromatics10-18 min : C3/C4 naphtalenes18-25 min : C1 fluorenes dibenzothiophenes25-28 min : phenanthrene

Arjuna (terrestrial o.m.) Boscan (marine o.m)

0-11 min : hexane 0,5 ml/min ; 11-15 min : hexane 1 ml/min15-25 min : 1 ml/min gradient hexane /DCM 80/20 ; 25-35 min : 1 ml/min gradient DCM 100 UV detection 254 nm (GC/MS identification)

Courtesy of Dr Filoche R&D dept of RP Vitry - France

Courtesy of Mme Vandenbroucke IFP


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4,5 B1,NO35,8 C7,6 PP11,3 B6,HCl13,8 B517 B2 base

Hydrosoluble vitaminsUptisphere 120 Å, 5 µ C18-HDO, 250 x 4.6 mm

Vitamins from serum extractUptisphere 120 Å, 5 µ C18-ODB, 250 x 4.6 mm

OPA Amino acidsUptisphere 120 Å 5 µ C18-HDO, 250 x 4.6 mm

Hydrosoluble vitaminsUptisphere 120 Å 5 µ C18-ODB, 150 x 4.6 mm

MeOH 100%1 ml/minUV 295 nm

A : (50 mM Acetate Na pH : 5,7) ; 3% THFB : 100% MeOH ; 0.05 THFFlow : 1 ml/min ; 37 °C - inj : 250 pmole of each AAFluo : ex : 346 nm . em : 455 nm

1- Riboflavin2- Nicotinamide3- Pyridoxine

1-L-cysteic acid2-ASP3-Glu4-ASN5-His6-Ser7-Gln8-ARG9-Gly10-THR 11-Taurine12-Ala13-Tyr14-Met15-Trp16-Val17-Phe18-Ile19-Leu20-Lys

A : ACNB : 0,05M Buffer (pH : 2,6)0 to 20 min : 0% A20 to 23 min : 40% A23 to 40 min : 0% A

UV 268 nm 0 to 6 minUV 292 nm from 6 to 11 minUV 205 nm from 11 to 14 minUV 268 after 14 min

5,65 Vitamin A7,61 Ac retinol15,93 Vitamin E

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Amino acidsUptisphere 120 Å 5 µ C18-HDO, 250 x 4.6 mm

Amino acidsUptisphere 120 Å 3 µ C18-HDO, 150 x 4.6 mm

Molbile Phase : CH3CN/H2O - 75/25Temp. : 35°CFlow rate : 1.5 ml /minInjection : 1 µlDetector : RI

Gains :• same resolution• increased productivity• less solvent consumption

Analysis time for the last peak : b- Lys : 56.55 min Analysis time for the last peak : b- Lys : 31.58 min

Sugars in strong alcoholUptisphere 5 µ NH2, 200 x 4.6 mm


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PAH's mixture (610)Uptisphere 5 µ TF, 250 x 4.6 mm

Solvent A :ACN:H2O, 50:50, v/vSolvent B : ACNFlow rate : 1.0 ml/minColumn temp. : 30°CGradient : Min %A0.0 80.05.0 80.030.0 1.035.0 1.036.0 80.040.0 80.0Detection : FL ex 270 nm, em 380 nm

Mobile Phase : 999/1 ACN/MeOHFlow rate : 0.5 ml/mmTemp. 30°CInjection 200 µl (~ 40 ng of D3)

Vitamin D3Uptisphere 5 µ TF, 250 x 4.6 mm

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DipyridoneUptisphere 5 µ HDO, 100 x 4.6 mm

CatecholamineStrategy™ 5 µ C18-2, 250 x 4.6 mm

Sample tr As N Rs

4-Hydroxy-3-methoxymandelic acid 3.59 0.93 13611.09 0DL-threodihydroxy-phenyl serine 7.99 1.08 14845.64 22.842-Acetamidophenol 9.28 1.02 17871.29 4.81DL-noradrenaline 15.45 1.02 18212.13 16.75adrenaline 18.36 1.01 16933.1 5.71DL-octapamin e 20.75 1.01 18519.6 4.07dopamine 25.33 0.99 18129.81 6.72

ACN/H2O 20/80pH 2 + 30mM sodiumn-octylsulfonic acid1mL/min220nm


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Analysis - HPLC - Interchim technologyReferences

Selection of publications with Uptisphere® columns

Uptisphere® 5 µm C18-ODBMarjorie Chèze and all, Laboratoire TOXLAB, 7 rue Jacques Cartier, F-75018 Paris, France ; Science Direct, Forensic Science International 153 (2005) 3–10 ; Hair analysis by liquid chromatography–tandem mass spectrometry in toxicological investigation of drug-facilitated crimes: Report of 128 cases over the period June 2003–May 2004 in metropolitan Paris.

Uptisphere® 5 µm C18-ODBHend Bejaoui and all, Laboratoire de Génie Chimique UMR5503 (CNRS-INPT-UPS), Département: Bioprocédés et Systèmes Microbiens, ENSAT/INPT : 1, Auzeville-Tolo-zane, Castanet-Tolosan, France ; FEMS Microbiol Lett 255 (2006) 203–208, Biodegradation of ochratoxin A by Aspergillussection Nigri Species isolated from French grapes : a potential means of ochratoxin A decontamination in grape juices and musts.

Uptisphere® 5 µm C18 BERTRAND GAKIÈRE, LAURENCE DENIS, MICHEL DROUX, STEPHANE RAVANEL, DOMINIQUE JOB; Laboratoire mixte CNRS-RPA (UMR1932), 14-20 rue Pierre Baizet,69263 Lyon Cedex 9, France ; METHIONINE SYNTHESIS IN HIGHER PLANTS: SENSE STRATEGY APPLIED TO CYSTATHIONINE γ-SYNTHASE AND CYS-TATHIONINE β-LYASE IN ARABIDOPSIS THALIANA

Uptisphere® 5 µm C18-ODB, C18-HDO, C18-NEC, C18-TF, C18-HSCCinzia Stella (a,b), Serge Rudaz (a,b), Jean-Yves Gauvrit (c), Pierre Lantéri (c), Alban Huteau (d), Alain Tchapla (d), Jean-Luc Veuthey (a), (a)Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, University of Geneva, Switzerland, (b)Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, University of Lausanne, Switzerland, (c)Laboratory of Chemometrics, ESPCE Lyon, University Claude Bernard Lyon I, Villeurbanne, France, (d)Groupe de Chimie, Analytique de Paris Sud, EA 33-43 LETIAM IUT d’Orsay, Université Paris XI, Orsay, France ; Science Direct, Journal of Pharmaceutical and Biomedical Analysis, xxx (2006) xxx-xxx, Characterization and comparison of the chromatographic performance of different types of reversed-phase stationary phases.

Uptisphere® 5 µm C18-ODB, C18-HDO, C18-NEC, C18-TF, C18-HSC Lesellier , Alain Tchapla Groupe de Chimie Analytique de Paris Sud, LETIAM IUT d’Orsay EA 3343, Plateau du Moulon, F-91400 Orsay, France Received 9 May 2005; recei-ved in revised form 5 September 2005; accepted 9 September 2005. A simple subcritical chromatographic test for an extended ODS high performance liquid chromatography column classification

Uptisphere® 5 µm C18-NECSouleiman El Balkh and all, Laboratoire de Toxicologie Biologique, Hopital Lariboisiere, 2 rue Ambroise Paré, 75475 Paris, France ; Journal of Analytical Toxicology, Vol. 29,May / June 2005, Determination of Ethylene Thiourea in Urine by HPLC-DAD

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Analysis - HPLC - Interchim technologyInterchrom columns

Interchrom HPLC columnsInterchrom HPLC columns combine the benefits associated with Modulo-Cart hardware (see P. A28) with a range of original proprietary stationary phases from different manufacturers, highlighted on the following page.

The Interchrom range has been in production at Interchim's facilities for over 30 years. Each column is supplied with a report similar to that shown to guarantee column integrity. Column specific reports detail :

Reference & Column number Stationary phase profile Column dimensions Verification of column performance, under stated test conditions Column storage solvent

Our whole range of Interchrom columns are shipped within 24 hours of ordering.

Interchrom columns may be available with the stationary phase /column dimensions of your choice. Please enquire.

Interchrom HPLC column guarantee

1. Interchrom columns always utilize the original manufacturer stationary phase relative to the patented brand owner.2. Each phase batch undergoes strict quality control.3. Every Modulo-Cart Quick Seal column is individually tested and delivered with its own chromatogram certificate.4. Every Modulo-Cart Quick Seal produced fits the companies stringent standards of production.5. Every Modulo-Cart Quick Seal is shipped within 24 working hours of receipt of order.


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Modulo-cart QS & QK Ø int. : 4.6 mm Ø int. : 4.0 mm Ø int. : 3.0 mm Ø int. : 2.0 mm 250 mm 150 mm 100 mm 50 mm 250 mm 150 mm 125 mm 250 mm 150 mm 100 mm 50 mm 250 mm 150 mm 100 mm 50 mm

Develosil x x x x x x x x x xInertsil series II x x x x x x x x x x xInertsil series III x x x x x x x x x x xKromasil x x x x x x x x x x x x x x xLichrosorb x x x x x Lichrospher x x x x x x Nucléosil x x x x x x x x x x Partisil x x x x Siligel x x x x x x x x x x Superspher x x x x x Yperspher x x x x x x x x x Zorbax x x x x

Column specifications

QS QK

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Octadecyl C18 3 µm 50 x 2.0 mm DV3C18#3QS DV3C18#3QK100Å - 350 m2/g C18 3 µm 100 x 2.0 mm DV3C18#10QS DV3C18#10QK%C : 20 C18 3 µm 150 x 2.0 mm DV3C18#13QS DV3C18#13QKend-capped C18 3 µm 150 x 3.0 mm DV3C18$13QS DV3C18$13QK3.1 µmol/m2 C18 3 µm 50 x 4.6 mm DV3C18-3QS DV3C18-3QK C18 3 µm 100 x 4.6 mm DV3C18-10QS DV3C18-10QK C18 3 µm 150 x 4.6 mm DV3C18-13QS DV3C18-13QK C18 5 µm 50 x 2.0 mm DV5C18#5QS DV5C18#5QK C18 5 µm 100 x 2.0 mm DV5C18#10QS DV5C18#10QK C18 5 µm 150 x 2.0 mm DV5C18#15QS DV5C18#15QK C18 5 µm 250 x 2.0 mm DV5C18#25QS DV5C18#25QK C18 5 µm 150 x 3.0 mm DV5C18$15QS DV5C18$15QK C18 5 µm 250 x 3.0 mm DV5C18$25QS DV5C18$25QK C18 5 µm 50 x 4.6 mm DV5C18-5QS DV5C18-5QK C18 5 µm 100 x 4.6 mm DV5C18-10QS DV5C18-10QK C18 5 µm 150 x 4.6 mm DV5C18-15QS DV5C18-15QK C18 5 µm 250 x 4.6 mm DV5C18-25QS DV5C18-25QK Octyl C8 5 µm 50 x 2.0 mm DV5C8#5QS DV5C8#5QK100Å - 350 m2/g C8 5 µm 100 x 2.0 mm DV5C8#10QS DV5C8#10QK%C : 13 C8 5 µm 150 x 2.0 mm DV5C8#15QS DV5C8#15QKend-capped C8 5 µm 250 x 2.0 mm DV5C8#25QS DV5C8#25QK3.4 µmol/m2 C8 5 µm 150 x 3.0 mm DV5C8$15QS DV5C8$15QK C8 5 µm 250 x 3.0 mm DV5C8$25QS DV5C8$25QK C8 5 µm 50 x 4.6 mm DV5C8-5QS DV5C8-5QK C8 5 µm 100 x 4.6 mm DV5C8-10QS DV5C8-10QK C8 5 µm 150 x 4.6 mm DV5C8-15QS DV5C8-15QK C8 5 µm 250 x 4.6 mm DV5C8-25QS DV5C8-25QK Methyl C1 5 µm 50 x 2.0 mm DV5C1#5QS DV5C1#5QK100Å - 350 m2/g C1 5 µm 100 x 2.0 mm DV5C1#10QS DV5C1#10QK%C : 5 C1 5 µm 150 x 2.0 mm DV5C1#15QS DV5C1#15QK4.2 µmol/m2 C1 5 µm 250 x 2.0 mm DV5C1#25QS DV5C1#25QK C1 5 µm 150 x 3.0 mm DV5C1$15QS DV5C1$15QK C1 5 µm 250 x 3.0 mm DV5C1$25QS DV5C1$25QK C1 5 µm 50 x 4.6 mm DV5C1-5QS DV5C1-5QK C1 5 µm 100 x 4.6 mm DV5C1-10QS DV5C1-10QK C1 5 µm 150 x 4.6 mm DV5C1-15QS DV5C1-15QK C1 5 µm 250 x 4.6 mm DV5C1-25QS DV5C1-25QK

Silica manufacturer : Nomura Chemical

Standard silica type A Spherical Surface area : 350 m2/g pH stability: 2 < pH < 7,5

Develosil® 100 Å

QS QK


A.97

B.97



Cyano CN 5 µm 50 x 2.0 mm DV5CN#5QS DV5CN#5QK100Å - 350 m2/g CN 5 µm 100 x 2.0 mm DV5CN#10QS DV5CN#10QK CN 5 µm 150 x 2.0 mm DV5CN#15QS DV5CN#15QK CN 5 µm 250 x 2.0 mm DV5CN#25QS DV5CN#25QK CN 5 µm 150 x 3.0 mm DV5CN$15QS DV5CN$15QK CN 5 µm 250 x 3.0 mm DV5CN$25QS DV5CN$25QK CN 5 µm 50 x 4.6 mm DV5CN-5QS DV5CN-5QK CN 5 µm 100 x 4.6 mm DV5CN-10QS DV5CN-10QK CN 5 µm 150 x 4.6 mm DV5CN-15QS DV5CN-15QK CN 5 µm 250 x 4.6 mm DV5CN-25QS DV5CN-25QK Amino NH2 5 µm 50 x 2.0 mm DV5NH2#5QS DV5NH2#5QK100Å - 350 m2/g NH2 5 µm 100 x 2.0 mm DV5NH2#10QS DV5NH2#10QK%C : 3 NH2 5 µm 150 x 2.0 mm DV5NH2#15QS DV5NH2#15QKnon end-capped NH2 5 µm 250 x 2.0 mm DV5NH2#25QS DV5NH2#25QK2.6 µmol/m2 NH2 5 µm 150 x 3.0 mm DV5NH2$15QS DV5NH2$15QK NH2 5 µm 250 x 3.0 mm DV5NH2$25QS DV5NH2$25QK NH2 5 µm 50 x 4.6 mm DV5NH2-5QS DV5NH2-5QK NH2 5 µm 100 x 4.6 mm DV5NH2-10QS DV5NH2-10QK NH2 5 µm 150 x 4.6 mm DV5NH2-15QS DV5NH2-15QK NH2 5 µm 250 x 4.6 mm DV5NH2-25QS DV5NH2-25QK Phenyl PH 5 µm 50 x 2.0 mm DV5PH#5QS DV5PH#5QK100Å - 350 m2/g PH 5 µm 100 x 2.0 mm DV5PH#10QS DV5PH#10QK%C : 10 PH 5 µm 150 x 2.0 mm DV5PH#15QS DV5PH#15QKend-capped PH 5 µm 250 x 2.0 mm DV5PH#25QS DV5PH#25QK3.1 µmol/m2 PH 5 µm 150 x 3.0 mm DV5PH$15QS DV5PH$15QK PH 5 µm 250 x 3.0 mm DV5PH$25QS DV5PH$25QK PH 5 µm 50 x 4.6 mm DV5PH-5QS DV5PH-5QK PH 5 µm 100 x 4.6 mm DV5PH-10QS DV5PH-10QK PH 5 µm 150 x 4.6 mm DV5PH-15QS DV5PH-15QK PH 5 µm 250 x 4.6 mm DV5PH-25QS DV5PH-25QK Silica SI 5 µm 50 x 2.0 mm DV5#5QS DV5#5QK100Å - 350 m2/g SI 5 µm 100 x 2.0 mm DV5#10QS DV5#10QK SI 5 µm 150 x 2.0 mm DV5#15QS DV5#15QK SI 5 µm 250 x 2.0 mm DV5#25QS DV5#25QK SI 5 µm 150 x 3.0 mm DV5$15QS DV5$15QK SI 5 µm 250 x 3.0 mm DV5$25QS DV5$25QK SI 5 µm 50 x 4.6 mm DV5-5QS DV5-5QK SI 5 µm 100 x 4.6 mm DV5-10QS DV5-10QK SI 5 µm 150 x 4.6 mm DV5-15QS DV5-15QK SI 5 µm 250 x 4.6 mm DV5-25QS DV5-25QK

Develosil® 100 Å

Column : Develosil ODS-5 (4,6 I.D. x 250 mm)Solvent : 70 % CH3OH - 30 % H2OFlow rate : 1.0 ml/minTemperature : 30°CDetection : UV254 nm

1. Methyl P-Hydroxy-benzoate2. Ethyl P-Hydroxyben-zoate3. Benzene4 . Toluene5. Naphthalene6. Ethylbenzene

Silica manufacturer : Nomura Chemical

Standard silica type A Spherical Surface area : 350 m2/g pH stability: 2 < pH < 7,5

A.98

B.98



Octadecyl OD2 5 µm 50 x 2.0 mm IN5OD2#5QS IN5OD2#5QK150Å - 320 m2/g OD2 5 µm 100 x 2.0 mm IN5OD2#10QS IN5OD2#10QK%C : 18,5 OD2 5 µm 150 x 2.0 mm IN5OD2#15QS IN5OD2#15QKend-capped OD2 5 µm 250 x 2.0 mm IN5OD2#25QS IN5OD2#25QK3.22 µmol/m2 OD2 5 µm 50 x 3.0 mm IN5OD2$5QS IN5OD2$5QK OD2 5 µm 150 x 3.0 mm IN5OD2$15QS IN5OD2$15QK OD2 5 µm 250 x 3.0 mm IN5OD2$25QS IN5OD2$25QK OD2 5 µm 50 x 4.6 mm IN5OD2-5QS IN5OD2-5QK OD2 5 µm 100 x 4.6 mm IN5OD2-10QS IN5OD2-10QK OD2 5 µm 150 x 4.6 mm IN5OD2-15QS IN5OD2-15QK OD2 5 µm 250 x 4.6 mm IN5OD2-25QS IN5OD2-25QK Octyl C8 5 µm 50 x 2.0 mm IN5C8#5QS IN5C8#5QK150Å - 320 m2/g C8 5 µm 100 x 2.0 mm IN5C8#10QS IN5C8#10QK%C : 10,5 C8 5 µm 150 x 2.0 mm IN5C8#15QS IN5C8#15QKend-capped C8 5 µm 250 x 2.0 mm IN5C8#25QS IN5C8#25QK3.26 µmol/m2 C8 5 µm 50 x 3.0 mm IN5C8$5QS IN5C8$5QK C8 5 µm 150 x 3.0 mm IN5C8$15QS IN5C8$15QK C8 5 µm 250 x 3.0 mm IN5C8$25QS IN5C8$25QK C8 5 µm 50 x 4.6 mm IN5C8-5QS IN5C8-5QK C8 5 µm 100 x 4.6 mm IN5C8-10QS IN5C8-10QK C8 5 µm 150 x 4.6 mm IN5C8-15QS IN5C8-15QK C8 5 µm 250 x 4.6 mm IN5C8-25QS IN5C8-25QK Butyl C4 5 µm 50 x 2.0 mm IN5C4#5QS IN5C4#5QK150Å - 320 m2/g C4 5 µm 100 x 2.0 mm IN5C4#10QS IN5C4#10QK%C : 7,5 C4 5 µm 150 x 2.0 mm IN5C4#15QS IN5C4#15QKend-capped C4 5 µm 250 x 2.0 mm IN5C4#25QS IN5C4#25QK3.76 µmol/m2 C4 5 µm 50 x 3.0 mm IN5C4$5QS IN5C4$5QK C4 5 µm 150 x 3.0 mm IN5C4$15QS IN5C4$15QK C4 5 µm 250 x 3.0 mm IN5C4$25QS IN5C4$25QK C4 5 µm 50 x 4.6 mm IN5C4-5QS IN5C4-5QK C4 5 µm 100 x 4.6 mm IN5C4-10QS IN5C4-10QK C4 5 µm 150 x 4.6 mm IN5C4-15QS IN5C4-15QK C4 5 µm 250 x 4.6 mm IN5C4-25QS IN5C4-25QK

Inertsil® 150 Å

QS QK

Silica manufacturer : GL Sciences Inc.

Ultra pure silica 99.999 % Spherical Surface area : 320 m2/g pH stability : 2 < pH < 9


A.99

B.99



Phenyl PH 5 µm 50 x 2.0 mm IN5PH#5QS IN5PH#5QK150Å - 320 m2/g PH 5 µm 100 x 2.0 mm IN5PH#10QS IN5PH#10QK%C : 10 PH 5 µm 150 x 2.0 mm IN5PH#15QS IN5PH#15QKend-capped PH 5 µm 250 x 2.0 mm IN5PH#25QS IN5PH#25QK2.77 µmol/m2 PH 5 µm 50 x 3.0 mm IN5PH$5QS IN5PH$5QK PH 5 µm 150 x 3.0 mm IN5PH$15QS IN5PH$15QK PH 5 µm 250 x 3.0 mm IN5PH$25QS IN5PH$25QK PH 5 µm 50 x 4.6 mm IN5PH-5QS IN5PH-5QK PH 5 µm 100 x 4.6 mm IN5PH-10QS IN5PH-10QK PH 5 µm 150 x 4.6 mm IN5PH-15QS IN5PH-15QK PH 5 µm 250 x 4.6 mm IN5PH-25QS IN5PH-25QK Silica SI 5 µm 50 x 2.0 mm IN5#5QS IN5#5QK150Å - 320 m2/g SI 5 µm 100 x 2.0 mm IN5#10QS IN5#10QK SI 5 µm 150 x 2.0 mm IN5#15QS IN5#15QK SI 5 µm 250 x 2.0 mm IN5#25QS IN5#25QK SI 5 µm 50 x 3.0 mm IN5$5QS IN5$5QK SI 5 µm 150 x 3.0 mm IN5$15QS IN5$15QK SI 5 µm 250 x 3.0 mm IN5$25QS IN5$25QK SI 5 µm 50 x 4.6 mm IN5-5QS IN5-5QK SI 5 µm 100 x 4.6 mm IN5-10QS IN5-10QK SI 5 µm 150 x 4.6 mm IN5-15QS IN5-15QK SI 5 µm 250 x 4.6 mm IN5-25QS IN5-25QK

Inertsil® 150 Å

QS QK


Ultra pure silica 99.999 % Spherical Surface area : 320 m2/g pH stability : 2 < pH < 9

A.100

B.100



Octadecyl ODS-3 3 µm 50 x 2.0 mm IN3OD3#5QS IN3OD3#5QK100Å - 450 m2/g ODS-3 3 µm 100 x 2.0 mm IN3OD3#10QS IN3OD3#10QK%C : 15 ODS-3 3 µm 150 x 2.0 mm IN3OD3#15QS IN3OD3#15QKend-capped ODS-3 3 µm 50 x 3.0 mm IN3OD3$5QS IN3OD3$5QK µmol/m2 ODS-3 3 µm 150 x 3.0 mm IN3OD3$15QS IN3OD3$15QK ODS-3 3 µm 50 x 4.6 mm IN3OD3-5QS IN3OD3-5QK ODS-3 3 µm 100 x 4.6 mm IN3OD3-10QS IN3OD3-10QK ODS-3 3 µm 150 x 4.6 mm IN3OD3-15QS IN3OD3-15QK ODS-3 3 µm 250 x 4.6 mm IN3OD3-25QS IN3OD3-25QK ODS-3 5 µm 50 x 2.0 mm IN5OD3#5QS IN5OD3#5QK ODS-3 5 µm 100 x 2.0 mm IN5ODS3#10QS IN5ODS3#10QK ODS-3 5 µm 150 x 2.0 mm IN5OD3#15QS IN5OD3#15QK ODS-3 5 µm 250 x 2.0 mm IN5OD3#25QS IN5OD3#25QK ODS-3 5 µm 50 x 3.0 mm IN5OD3$5QS IN5OD3$5QK ODS-3 5 µm 150 x 3.0 mm IN5OD3$15QS IN5OD3$15QK ODS-3 5 µm 250 x 3.0 mm IN5OD3$25QS IN5OD3$25QK ODS-3 5 µm 50 x 4.6 mm IN5OD3-5QS IN5OD3-5QK ODS-3 5 µm 100 x 4.6 mm IN5OD3-10QS IN5OD3-10QK ODS-3 5 µm 150 x 4.6 mm IN5OD3-15QS IN5OD3-15QK ODS-3 5 µm 250 x 4.6 mm IN5OD3-25QS IN5OD3-25QK Octyl C8-3 3 µm 50 x 2.0 mm IN3C83#5QS IN3C83#5QK100Å - 450 m2/g C8-3 3 µm 100 x 2.0 mm IN3C83#10QS IN3C83#10QK%C : 9 C8-3 3 µm 150 x 2.0 mm IN3C83#15QS IN3C83#15QKend-capped C8-3 3 µm 50 x 4.6 mm IN3C83-5QS IN3C83-5QK C8-3 3 µm 100 x 4.6 mm IN3C83-10QS IN3C83-10QK C8-3 3 µm 150 x 4.6 mm IN3C83-15QS IN3C83-15QK C8-3 5 µm 50 x 2.0 mm IN5C83#5QS IN5C83#5QK C8-3 5 µm 100 x 2.0 mm IN5C83#10QS IN5C83#10QK C8-3 5 µm 150 x 2.0 mm IN5C83#15QS IN5C83#15QK C8-3 5 µm 250 x 2.0 mm IN5C83#25QS IN5C83#25QK C8-3 5 µm 50 x 3.0 mm IN5C83$5QS IN5C83$5QK C8-3 5 µm 150 x 3.0 mm IN5C83$15QS IN5C83$15QK C8-3 5 µm 250 x 3.0 mm IN5C83$25QS IN5C83$25QK C8-3 5 µm 50 x 4.6 mm IN5C83-5QS IN5C83-5QK C8-3 5 µm 100 x 4.6 mm IN5C83-10QS IN5C83-10QK C8-3 5 µm 150 x 4.6 mm IN5C83-15QS IN5C83-15QK C8-3 5 µm 250 x 4.6 mm IN5C83-25QS IN5C83-25QK

Inertsil® 100 Å


Ultra pure silica 99,999 % Spherical Surface area : 450 m2/g pH stability : 1 < pH < 9

Aromatic Organic acid

Column : Inertsil ODS-80A, 5 µm, 150 x 4.6 mmEluant : CH3CN/0,1 % H3PO4 = 15/85Flow rate : 1.0 ml/minDetection : UV 254 nmCol. Temp. : 40°C

Sample :1. Gallic acid2. 3,4-Dihydroxy phenyl acetic acid3. p-Hydroxy benzoic acid4. Caffeic acid5. Vanillic acid6. Gentisic acid7. p-Coumaric acid8. Ferulic acid9. m-Coumaric acid10. o-Coumamric acid11. Salicylic acid (each 5ppm)


A.101

B.101



Cyano CN-3 3 µm 50 x 2.0 mm IN3CN3#5QS IN3CN3#5QK100Å - 450 m2/g CN-3 3 µm 100 x 2.0 mm IN3CN3#10QS IN3CN3#10QK%C : 14 CN-3 3 µm 150 x 2.0 mm IN3CN3#15QS IN3CN3#15QKnon end-capped CN-3 3 µm 50 x 4.6 mm IN3CN3-5QS IN3CN3-5QK CN-3 3 µm 100 x 4.6 mm IN3CN3-10QS IN3CN3-10QK CN-3 3 µm 150 x 4.6 mm IN3CN3-15QS IN3CN3-15QK CN-3 5 µm 50 x 2.0 mm IN5CN3#5QS IN5CN3#5QK CN-3 5 µm 100 x 2.0 mm IN5CN3#10QS IN5CN3#10QK CN-3 5 µm 150 x 2.0 mm IN5CN3#15QS IN5CN3#15QK CN-3 5 µm 250 x 2.0 mm IN5CN3#25QS IN5CN3#25QK CN-3 5 µm 50 x 3.0 mm IN5CN3$5QS IN5CN3$5QK CN-3 5 µm 150 x 3.0 mm IN5CN3$15QS IN5CN3$15QK CN-3 5 µm 250 x 3.0 mm IN5CN3$25QS IN5CN3$25QK CN-3 5 µm 50 x 4.6 mm IN5CN3-5QS IN5CN3-5QK CN-3 5 µm 150 x 4.6 mm IN5CN3-15QS IN5CN3-15QK CN-3 5 µm 100 x 4.6 mm IN5CN3-10QS IN5CN3-10QK CN-3 5 µm 250 x 4.6 mm IN5CN3-25QS IN5CN3-25QK Phenyl PH-3 3 µm 50 x 2.0 mm IN3PH3#5QS IN3PH3#5QK100Å - 450 m2/g PH-3 3 µm 100 x 2.0 mm IN3PH3#10QS IN3PH3#10QK%C : 9,5 PH-3 3 µm 150 x 2.0 mm IN3PH3#15QS IN3PH3#15QKend-capped PH-3 3 µm 50 x 4.6 mm IN3PH3-5QS IN3PH3-5QK PH-3 3 µm 100 x 4.6 mm IN3PH3-10QS IN3PH3-10QK PH-3 3 µm 150 x 4.6 mm IN3PH3-15QS IN3PH3-15QK PH-3 5 µm 100 x 2.0 mm IN5PH3#10QS IN5PH3#10QK PH-3 5 µm 100 x 4.6 mm IN5PH3-10QS IN5PH3-10QK PH-3 5 µm 150 x 2.0 mm IN5PH3#15QS IN5PH3#15QK PH-3 5 µm 150 x 3.0 mm IN5PH3$15QS IN5PH3$15QK PH-3 5 µm 150 x 4.6 mm IN5PH3-15QS IN5PH3-15QK PH-3 5 µm 250 x 2.0 mm IN5PH3#25QS IN5PH3#25QK PH-3 5 µm 250 x 3.0 mm IN5PH3$25QS IN5PH3$25QK PH-3 5 µm 250 x 4.6 mm IN5PH3-25QS IN5PH3-25QK PH-3 5 µm 50 x 2.0 mm IN5PH3#5QS IN5PH3#5QK PH-3 5 µm 50 x 3.0 mm IN5PH3$5QS IN5PH3$5QK PH-3 5 µm 50 x 4.6 mm IN5PH3-5QS IN5PH3-5QK

Inertsil® 100 Å

Melamine

Column : Inertsil Ph 5 µm, 150 x 4.6 mmEluant : 5mM IPCC-08 + 0.05 % - H3PO4 (pH 1,9)IPPC-08 : Sodium 1-OctanesulfonateFlow rate : 1.0 ml/minDetection : UV 195 nmCol. Temp. : 40°C

Sample :1. Cyanuric acid2. Ammelide3. Ammeline4. Melamine


Ultra pure silica 99,999 % Spherical Surface area : 450 m2/g pH stability : 1 < pH < 9

A.102

B.102



Octadecyl C18 3 µm 50 x 2.0 mm KR3C18#5QS KR3C18#5QK100Å - 330 m2/g C18 3 µm 100 x 2.0 mm KR3C18#10QS KR3C18#10QK%C : 20 C18 3 µm 150 x 2.0 mm KR3C18#15QS KR3C18#15QKend-capped C18 3 µm 50 x 3.0 mm KR3C18$5QS KR3C18$5QK3.5 µmol/m2 C18 3 µm 100 x 3.0 mm KR3C18$10QS KR3C18$10QK C18 3 µm 150 x 3.0 mm KR3C18$15QS KR3C18$15QK C18 3 µm 125 x 4.0 mm KR3C18*12QS KR3C18*12QK C18 3 µm 150 x 4.0 mm KR3C18*15QS KR3C18*15QK C18 3 µm 50 x 4.6 mm KR3C18-5QS KR3C18-5QK C18 3 µm 100 x 4.6 mm KR3C18-10QS KR3C18-10QK C18 3 µm 150 x 4.6 mm KR3C18-15QS KR3C18-15QK C18 5 µm 50 x 2.0 mm KR5C18#5QS KR5C18#5QK C18 5 µm 100 x 2.0 mm KR5C18#10QS KR5C18#10QK C18 5 µm 150 x 2.0 mm KR5C18#15QS KR5C18#15QK C18 5 µm 250 x 2.0 mm KR5C18#25QS KR5C18#25QK C18 5 µm 50 x 3.0 mm KR5C18$5QS KR5C18$5QK C18 5 µm 100 x 3.0 mm KR5C18$10QS KR5C18$10QK C18 5 µm 150 x 3.0 mm KR5C18$15QS KR5C18$15QK C18 5 µm 250 x 3.0 mm KR5C18$25QS KR5C18$25QK C18 5 µm 125 x 4.0 mm KR5C18*12QS KR5C18*12QK C18 5 µm 150 x 4.0 mm KR5C18*15QS KR5C18*15QK C18 5 µm 250 x 4.0 mm KR5C18*25QS KR5C18*25QK C18 5 µm 50 x 4.6 mm KR5C18-5QS KR5C18-5QK C18 5 µm 100 x 4.6 mm KR5C18-10QS KR5C18-10QK C18 5 µm 150 x 4.6 mm KR5C18-15QS KR5C18-15QK C18 5 µm 250 x 4.6 mm KR5C18-25QS KR5C18-25QK C18 10 µm 250 x 4.6 mm KR10C18-25QS KR10C18-25QK Octyl C8 5 µm 50 x 2.0 mm KR5C8#5QS KR5C8#5QK100Å - 330 m2/g C8 5 µm 100 x 2.0 mm KR5C8#10QS KR5C8#10QK%C : 12 C8 5 µm 150 x 2.0 mm KR5C8#15QS KR5C8#15QKend-capped C8 5 µm 250 x 2.0 mm KR5C8#25QS KR5C8#25QK3.7 µmol/m2 C8 5 µm 50 x 3.0 mm KR5C8$5QS KR5C8$5QK C8 5 µm 100 x 3.0 mm KR5C8$10QS KR5C8$10QK C8 5 µm 150 x 3.0 mm KR5C8$15QS KR5C8$15QK C8 5 µm 250 x 3.0 mm KR5C8$25QS KR5C8$25QK C8 5 µm 125 x 4.0 mm KR5C8*12QS KR5C8*12QK C8 5 µm 150 x 4.0 mm KR5C8*15QS KR5C8*15QK C8 5 µm 250 x 4.0 mm KR5C8*25QS KR5C8*25QK C8 5 µm 50 x 4.6 mm KR5C8-5QS KR5C8-5QK C8 5 µm 100 x 4.6 mm KR5C8-10QS KR5C8-10QK C8 5 µm 150 x 4.6 mm KR5C8-15QS KR5C8-15QK C8 5 µm 250 x 4.6 mm KR5C8-25QS KR5C8-25QK C8 10 µm 250 x 4.6 mm KR10C8-25QS KR10C8-25QK

Kromasil® 100 Å

QS QK

Silica manufacturer : EKA Chemicals

Standard silica type A Spherical Surface area : 340 m2/g pH stability : 1,5 < pH < 9,5


A.103

B.103



Butyl C4 5 µm 50 x 2.0 mm KR5C4#5QS KR5C4#5QK100Å - 330 m2/g C4 5 µm 100 x 2.0 mm KR5C4#10QS KR5C4#10QK%C : 8 C4 5 µm 150 x 2.0 mm KR5C4#15QS KR5C4#15QKend-capped C4 5 µm 250 x 2.0 mm KR5C4#25QS KR5C4#25QK3.8 µmol/m2 C4 5 µm 50 x 3.0 mm KR5C4$5QS KR5C4$5QK C4 5 µm 100 x 3.0 mm KR5C4$10QS KR5C4$10QK C4 5 µm 150 x 3.0 mm KR5C4$15QS KR5C4$15QK C4 5 µm 250 x 3.0 mm KR5C4$25QS KR5C4$25QK C4 5 µm 125 x 4.0 mm KR5C4*12QS KR5C4*12QK C4 5 µm 150 x 4.0 mm KR5C4*15QS KR5C4*15QK C4 5 µm 250 x 4.0 mm KR5C4*25QS KR5C4*25QK C4 5 µm 50 x 4.6 mm KR5C4-5QS KR5C4-5QK C4 5 µm 100 x 4.6 mm KR5C4-10QS KR5C4-10QK C4 5 µm 150 x 4.6 mm KR5C4-15QS KR5C4-15QK C4 5 µm 250 x 4.6 mm KR5C4-25QS KR5C4-25QK Methyl C1 5 µm 50 x 2.0 mm KR5C1#5QS KR5C1#5QK100Å - 330 m2/g C1 5 µm 100 x 2.0 mm KR5C1#10QS KR5C1#10QK%C : 5 C1 5 µm 150 x 2.0 mm KR5C1#15QS KR5C1#15QKend-capped C1 5 µm 250 x 2.0 mm KR5C1#25QS KR5C1#25QK4.2 µmol/m2 C1 5 µm 50 x 3.0 mm KR5C1$5QS KR5C1$5QK C1 5 µm 100 x 3.0 mm KR5C1$10QS KR5C1$10QK C1 5 µm 150 x 3.0 mm KR5C1$15QS KR5C1$15QK C1 5 µm 250 x 3.0 mm KR5C1$25QS KR5C1$25QK C1 5 µm 125 x 4.0 mm KR5C1*12QS KR5C1*12QK C1 5 µm 150 x 4.0 mm KR5C1*15QS KR5C1*15QK C1 5 µm 250 x 4.0 mm KR5C1*25QS KR5C1*25QK C1 5 µm 50 x 4.6 mm KR5C1-5QS KR5C1-5QK C1 5 µm 100 x 4.6 mm KR5C1-10QS KR5C1-10QK C1 5 µm 150 x 4.6 mm KR5C1-15QS KR5C1-15QK C1 5 µm 250 x 4.6 mm KR5C1-25QS KR5C1-25QK

Kromasil® 100 Å

QS QK


Standard silica type A Spherical Surface area : 340 m2/g pH stability : 1.5 < pH < 9.5

A.104

B.104



Cyano CN 5 µm 50 x 2.0 mm KR5CN#5QS KR5CN#5QK60Å - 530 m2/g CN 5 µm 100 x 2.0 mm KR5CN#10QS KR5CN#10QK%C : 12 CN 5 µm 150 x 2.0 mm KR5CN#15QS KR5CN#15QK%N : 2.3 CN 5 µm 250 x 2.0 mm KR5CN#25QS KR5CN#25QKend-capped CN 5 µm 50 x 3.0 mm KR5CN$5QS KR5CN$5QK3.8 µmol/m2 CN 5 µm 100 x 3.0 mm KR5CN$10QS KR5CN$10QK CN 5 µm 150 x 3.0 mm KR5CN$15QS KR5CN$15QK CN 5 µm 250 x 3.0 mm KR5CN$25QS KR5CN$25QK CN 5 µm 125 x 4.0 mm KR5CN*12QS KR5CN*12QK CN 5 µm 150 x 4.0 mm KR5CN*15QS KR5CN*15QK CN 5 µm 250 x 4.0 mm KR5CN*25QS KR5CN*25QK CN 5 µm 50 x 4.6 mm KR5CN-5QS KR5CN-5QK CN 5 µm 100 x 4.6 mm KR5CN-10QS KR5CN-10QK CN 5 µm 150 x 4.6 mm KR5CN-15QS KR5CN-15QK CN 5 µm 250 x 4.6 mm KR5CN-25QS KR5CN-25QK Amino NH2 5 µm 50 x 2.0 mm KR5NH2#5QS KR5NH2#5QK100Å - 330 m2/g NH2 5 µm 100 x 2.0 mm KR5NH2#10QS KR5NH2#10QK%N : 1.7 NH2 5 µm 150 x 2.0 mm KR5NH2#15QS KR5NH2#15QKend-capped NH2 5 µm 250 x 2.0 mm KR5NH2#25QS KR5NH2#25QK4.5 µmol/m2 NH2 5 µm 50 x 3.0 mm KR5NH2$5QS KR5NH2$5QK NH2 5 µm 100 x 3.0 mm KR5NH2$10QS KR5NH2$10QK NH2 5 µm 150 x 3.0 mm KR5NH2$15QS KR5NH2$15QK NH2 5 µm 250 x 3.0 mm KR5NH2$25QS KR5NH2$25QK NH2 5 µm 125 x 4.0 mm KR5NH2*12QS KR5NH2*12QK NH2 5 µm 150 x 4.0 mm KR5NH2*15QS KR5NH2*15QK NH2 5 µm 250 x 4.0 mm KR5NH2*25QS KR5NH2*25QK NH2 5 µm 50 x 4.6 mm KR5NH2-5QS KR5NH2-5QK NH2 5 µm 100 x 4.6 mm KR5NH2-10QS KR5NH2-10QK NH2 5 µm 150 x 4.6 mm KR5NH2-15QS KR5NH2-15QK NH2 5 µm 250 x 4.6 mm KR5NH2-25QS KR5NH2-25QK Silica SI 5 µm 50 x 2.0 mm KR5#5QS KR5#5QK100Å - 330 m2/g SI 5 µm 100 x 2.0 mm KR5#10QS KR5#10QK SI 5 µm 150 x 2.0 mm KR5#15QS KR5#15QK SI 5 µm 250 x 2.0 mm KR5#25QS KR5#25QK SI 5 µm 50 x 3.0 mm KR5$5QS KR5$5QK SI 5 µm 100 x 3.0 mm KR5$10QS KR5$10QK SI 5 µm 150 x 3.0 mm KR5$15QS KR5$15QK SI 5 µm 250 x 3.0 mm KR5$25QS KR5$25QK SI 5 µm 125 x 4.0 mm KR5*12QS KR5*12QK SI 5 µm 150 x 4.0 mm KR5*15QS KR5*15QK SI 5 µm 250 x 4.0 mm KR5*25QS KR5*25QK SI 5 µm 50 x 4.6 mm KR5-5QS KR5-5QK SI 5 µm 100 x 4.6 mm KR5-10QS KR5-10QK SI 5 µm 150 x 4.6 mm KR5-15QS KR5-15QK SI 5 µm 250 x 4.6 mm KR5-25QS KR5-25QK SI 10 µm 250 x 4.6 mm KR10-25QS KR10-25QK

Kromasil® 60 &100 Å

QS QK


Standard silica type A Spherical Surface area : 340 m2/g pH stability : 1.5 < pH < 9.5


A.105

B.105



Octadecyl C18 5 µm 125 x 4.0 mm L5C18*12QS L5C18*12QK100Å - 300 m2/g C18 5 µm 150 x 4.0 mm L5C18*15QS L5C18*15QK%C : 16.2 C18 5 µm 250 x 4.0 mm L5C18*25QS L5C18*25QKend-capped C18 10 µm 250 x 4.0 mm L10C18*25QS L10C18*25QK3 µmol/m2 C18 5 µm 150 x 4.6 mm L5C18-15QS L5C18-15QK C18 5 µm 250 x 4.6 mm L5C18-25QS L5C18-25QK C18 10 µm 250 x 4.6 mm L10C18-25QS L10C18-25QK Octyl C8 5 µm 125 x 4.0 mm L5C8*12QS L5C8*12QK100Å - 300 m2/g C8 5 µm 150 x 4.0 mm L5C8*15QS L5C8*15QK%C : 9.5 C8 5 µm 250 x 4.0 mm L5C8*25QS L5C8*25QKend-capped C8 5 µm 150 x 4.6 mm L5C8-15QS L5C8-15QK3.4 µmol/m2 C8 5 µm 250 x 4.6 mm L5C8-25QS L5C8-25QK Octyl RPB 5 µm 125 x 4.0 mm L5RPB*12QS L5RPB*12QK100Å - 300 m2/g RPB 5 µm 150 x 4.0 mm L5RPB*15QS L5RPB*15QK%C : 11.5 RPB 5 µm 250 x 4.0 mm L5RPB*25QS L5RPB*25QKend-capped RPB 5 µm 150 x 4.6 mm L5RPB-15QS L5RPB-15QK2.5 µmol/m2 RPB 5 µm 250 x 4.6 mm L5RPB-25QS L5RPB-25QK Cyano CN 5 µm 125 x 4.0 mm L5CN*12QS L5CN*12QK100Å - 300 m2/g CN 5 µm 150 x 4.0 mm L5CN*15QS L5CN*15QK%C : 6.1 CN 5 µm 250 x 4.0 mm L5CN*25QS L5CN*25QKend-capped CN 5 µm 150 x 4.6 mm L5CN-15QS L5CN-15QK3.82 µmol/m2 CN 5 µm 250 x 4.6 mm L5CN-25QS L5CN-25QK Diol OH 5 µm 125 x 4.0 mm L5OH*12QS L5OH*12QK100Å - 300 m2/g OH 5 µm 150 x 4.0 mm L5OH*15QS L5OH*15QK%C : 7.1 OH 5 µm 250 x 4.0 mm L5OH*25QS L5OH*25QK3.91 µmol/m2 OH 5 µm 150 x 4.6 mm L5OH-15QS L5OH-15QK OH 5 µm 250 x 4.6 mm L5OH-25QS L5OH-25QK Silica SI 5 µm 125 x 4.0 mm L5*12QS L5*12QK60Å - 500 m2/g SI 5 µm 150 x 4.0 mm L5*15QS L5*15QK SI 5 µm 250 x 4.0 mm L5*25QS L5*25QK SI 10 µm 250 x 4.0 mm L10*25QS L10*25QK SI 5 µm 150 x 4.6 mm L5-15QS L5-15QK

Lichrosorb® 60 &100 Å

Silica manufacturer : MERCK

Standard silica type A Surface area : 500 & 300 m2/g pH stability : 2 < pH < 7

Analysis of procyanidins of cone extracts I

Lichrospher RP18, 5µm (60 x 4.6 mm)Mobile phase : HCO2H2O (1.19) et (S’)CH2CNFlow rate : 2.5 ml/mnTemp. : 21°CPressure : 160 barsUV detector : 280 nm

A.106

B.106


Lichrospher® 60 &100 Å


Standard silica type A Spherical Surface area : 360 & 350 m2/g pH stability : 2 < pH < 7

QS QK


Octadecyl OD2 5 µm 125 x 4.0 mm K5OD2*12QS K5OD2*12QK100Å - 350 m2/g OD2 5 µm 150 x 4.0 mm K5OD2*15QS K5OD2*15QK%C : 21.6 OD2 5 µm 250 x 4.0 mm K5OD2*25QS K5OD2*25QKend-capped OD2 10 µm 250 x 4.0 mm K10OD2*25QS K10OD2*25QK4.09 µmol/m2 OD2 5 µm 100 x 4.6 mm K5OD2-10QS K5OD2-10QK OD2 5 µm 150 x 4.6 mm K5OD2-15QS K5OD2-15QK OD2 5 µm 250 x 4.6 mm K5OD2-25QS K5OD2-25QK OD2 10 µm 250 x 4.6 mm K10OD2-25QS K10OD2-25QK Octadecyl OD1 5 µm 125 x 4.0 mm K5OD1*12QS K5OD1*12QK100Å - 350 m2/g OD1 5 µm 150 x 4.0 mm K5OD1*15QS K5OD1*15QK%C : 21 OD1 5 µm 250 x 4.0 mm K5OD1*25QS K5OD1*25QKnon end-capped OD1 10 µm 250 x 4.0 mm K10OD1*25QS K10OD1*25QK3.61 µmol/m2 OD1 5 µm 100 x 4.6 mm K5OD1-10QS K5OD1-10QK OD1 5 µm 150 x 4.6 mm K5OD1-15QS K5OD1-15QK OD1 5 µm 250 x 4.6 mm K5OD1-25QS K10OD1-15QK OD1 10 µm 250 x 4.6 mm K10OD1-25QS K5OD1-25QK Octyl C8 5 µm 125 x 4.0 mm K5C8*12QS K5C8*12QK100Å - 350 m2/g C8 5 µm 150 x 4.0 mm K5C8*15QS K5C8*15QK%C : 12.5 C8 5 µm 250 x 4.0 mm K5C8*25QS K5C8*25QKnon end-capped C8 5 µm 100 x 4.6 mm K5C8-10QS K5C8-10QK4.04 µmol/m2 C8 5 µm 150 x 4.6 mm K5C8-15QS K5C8-15QK C8 5 µm 250 x 4.6 mm K5C8-25QS K5C8-25QK Octyl C8E 5 µm 125 x 4.0 mm K5C8E*12QS K5C8E*12QK100Å - 350 m2/g C8E 5 µm 150 x 4.0 mm K5C8E*15QS K5C8E*15QK%C : 13 C8E 5 µm 250 x 4.0 mm K5C8E*25QS K5C8E*25QKend-capped C8E 5 µm 100 x 4.6 mm K5C8E-10QS K5C8E-10QK4.44 µmol/m2 C8E 5 µm 150 x 4.6 mm K5C8E-15QS K5C8E-15QK C8E 5 µm 250 x 4.6 mm K5C8E-25QS K5C8E-25QK Octyl RPB 5 µm 125 x 4.0 mm K5RPB*12QS K5RPB*12QK60Å - 360 m2/g RPB 5 µm 150 x 4.0 mm K5RPB*15QS K5RPB*15QK%C : 11.5 RPB 5 µm 250 x 4.0 mm K5RPB*25QS K5RPB*25QKend-capped RPB 5 µm 100 x 4.6 mm K5RPB-10QS K5RPB-10QK3.55 µmol/m2 RPB 5 µm 150 x 4.6 mm K5RPB-15QS K5RPB-15QK RPB 5 µm 250 x 4.6 mm K5RPB-25QS K5RPB-25QK


A.107

B.107



Cyano CN 5 µm 125 x 4.0 mm K5CN*12QS K5CN*12QK100Å - 350 m2/g CN 5 µm 150 x 4.0 mm K5CN*15QS K5CN*15QK%C : 6,6 CN 5 µm 250 x 4.0 mm K5CN*25QS K5CN*25QK3.52 µmol/m2 CN 5 µm 100 x 4.6 mm K5CN-10QS K5CN-10QK CN 5 µm 150 x 4.6 mm K5CN-15QS K5CN-15QK CN 5 µm 250 x 4.6 mm K5CN-25QS K5CN-25QK Amino NH2 5 µm 125 x 4.0 mm K5NH2*12QS K5NH2*12QK100Å - 350 m2/g NH2 5 µm 150 x 4.0 mm K5NH2*15QS K5NH2*15QK%C : 4,6 NH2 5 µm 250 x 4.0 mm K5NH2*25QS K5NH2*25QK4.1 µmol/m2 NH2 5 µm 100 x 4.6 mm K5NH2-10QS K5NH2-10QK NH2 5 µm 150 x 4.6 mm K5NH2-15QS K5NH2-15QK NH2 5 µm 250 x 4.6 mm K5NH2-25QS K5NH2-25QK Diol OH 5 µm 125 x 4.0 mm K5OH*12QS K5OH*12QK100Å - 350 m2/g OH 5 µm 150 x 4.0 mm K5OH*15QS K5OH*15QK%C : 8 OH 5 µm 250 x 4.0 mm K5OH*25QS K5OH*25QK3.87 µmol/m2 OH 5 µm 100 x 4.6 mm K5OH-10QS K5OH-10QK OH 5 µm 150 x 4.6 mm K5OH-15QS K5OH-15QK OH 5 µm 250 x 4.6 mm K5OH-25QS K5OH-25QK Silica SI 5 µm 125 x 4.0 mm K5*12QS K5*12QK60Å - 700 m2/g SI 5 µm 150 x 4.0 mm K5*15QS K5*15QK SI 5 µm 250 x 4.0 mm K5*25QS K5*25QK SI 10 µm 250 x 4.0 mm K10*25QS K10*25QK SI 5 µm 100 x 4.6 mm K5-10QS K5-10QK SI 5 µm 150 x 4.6 mm K5-15QS K5-15QK SI 5 µm 250 x 4.6 mm K5-25QS K5-25QK SI 10 µm 250 x 4.6 mm K10-25QS K10-25QK

Lichrospher® 60 &100 Å

Column : Lichrospher Si 100 10 µmPressure : 124 bars (1800 psi)Flow rate : 300 ml/hourEluent : n-heptane Equipment : non commercial UV detector : 254nm

Sample :1. Benzene2. Diphenyl3. m-Terphenyl4. m-Quaterphenyl5. m-Quinquephenyl6. m-Sexiphenyl


Standard silica type A Spherical Surface area : 360 & 350 m2/g pH stability : 2 < pH < 7

A.108

B.108



Octadecyl C18 3 µm 150 x 2.0 mm N3C18#15QS N3C18#15QK100Å - 350 m2/g C18 3 µm 150 x 3.0 mm N3C18$15QS N3C18$15QK%C : 14 C18 3 µm 125 x 4.0 mm N3C18*12QS N3C18*12QKHMDS end-capping C18 3 µm 150 x 4.0 mm N3C18*15QS N3C18*15QK2.08 µmol/m2 C18 3 µm 100 x 4.6 mm N3C18-10QS N3C18-10QK C18 3 µm 150 x 4.6 mm N3C18-15QS N3C18-15QK C18 5 µm 150 x 2.0 mm N5C18#15QS N5C18#15QK C18 5 µm 250 x 2.0 mm N5C18#25QS N5C18#25QK C18 5 µm 150 x 3.0 mm N5C18$15QS N5C18$15QK C18 5 µm 250 x 3.0 mm N5C18$25QS N5C18$25QK C18 5 µm 125 x 4.0 mm N5C18*12QS N5C18*12QK C18 5 µm 150 x 4.0 mm N5C18*15QS N5C18*15QK C18 5 µm 250 x 4.0 mm N5C18*25QS N5C18*25QK C18 10 µm 250 x 4.0 mm N10C18*25QS N10C18*25QK C18 5 µm 100 x 4.6 mm N5C18-10QS N5C18-10QK C18 5 µm 150 x 4.6 mm N5C18-15QS N5C18-15QK C18 5 µm 250 x 4.6 mm N5C18-25QS N5C18-25QK C18 10 µm 250 x 4.6 mm N10C18-25QS N10C18-25QK Octyl C8 5 µm 150 x 2.0 mm N5C8#15QS N5C8#15QK100Å - 350 m2/g C8 5 µm 250 x 2.0 mm N5C8#25QS N5C8#25QK%C : 9 C8 5 µm 150 x 3.0 mm N5C8$15QS N5C8$15QKnon end-capped C8 5 µm 250 x 3.0 mm N5C8$25QS N5C8$25QK2.49 µmol/m2 C8 5 µm 125 x 4.0 mm N5C8*12QS N5C8*12QK C8 5 µm 150 x 4.0 mm N5C8*15QS N5C8*15QK C8 5 µm 250 x 4.0 mm N5C8*25QS N5C8*25QK C8 5 µm 100 x 4.6 mm N5C8-10QS N5C8-10QK C8 5 µm 150 x 4.6 mm N5C8-15QS N5C8-15QK C8 5 µm 250 x 4.6 mm N5C8-25QS N5C8-25QK Octyl C8E 5 µm 150 x 2.0 mm N5C8E#15QS N5C8E#15QK100Å - 350 m2/g C8E 5 µm 250 x 2.0 mm N5C8E#25QS N5C8E#25QK%C : 9 C8E 5 µm 150 x 3.0 mm N5C8E$15QS N5C8E$15QKHMDS end-capping C8E 5 µm 250 x 3.0 mm N5C8E$25QS N5C8E$25QK C8E 5 µm 125 x 4.0 mm N5C8E*12QS N5C8E*12QK C8E 5 µm 150 x 4.0 mm N5C8E*15QS N5C8E*15QK C8E 5 µm 250 x 4.0 mm N5C8E*25QS N5C8E*25QK C8E 5 µm 100 x 4.6 mm N5C8E-10QS N5C8E-10QK C8E 5 µm 150 x 4.6 mm N5C8E-15QS N5C8E-15QK C8E 5 µm 250 x 4.6 mm N5C8E-25QS N5C8E-25QK

Nucleosil®

Silica manufacturer : Macherey-Nagel

Standard silica type A Spherical Surface area : 350 m2/g pH stability : 2 < pH < 7

QS QK


A.109

B.109



Cyano CN 5 µm 150 x 2.0 mm N5CN#15QS N5CN#15QK100Å - 350 m2/g CN 5 µm 250 x 2.0 mm N5CN#25QS N5CN#25QK%C : 4 CN 5 µm 150 x 3.0 mm N5CN$15QS N5CN$15QKnon end-capped CN 5 µm 250 x 3.0 mm N5CN$25QS N5CN$25QK1.73 µmol/m2 CN 5 µm 125 x 4.0 mm N5CN*12QS N5CN*12QK CN 5 µm 150 x 4.0 mm N5CN*15QS N5CN*15QK CN 5 µm 250 x 4.0 mm N5CN*25QS N5CN*25QK CN 5 µm 100 x 4.6 mm N5CN-10QS N5CN-10QK CN 5 µm 150 x 4.6 mm N5CN-15QS N5CN-15QK CN 5 µm 250 x 4.6 mm N5CN-25QS N5CN-25QK Phenyl PH 5 µm 150 x 2.0 mm N5PH#15QS N5PH#15QK100Å - 350 m2/g PH 5 µm 250 x 2.0 mm N5PH#25QS N5PH#25QK%C : 8 PH 5 µm 150 x 3.0 mm N5PH$15QS N5PH$15QKnon end-capped PH 5 µm 250 x 3.0 mm N5PH$25QS N5PH$25QK1.96 µmol/m2 PH 5 µm 125 x 4.0 mm N5PH*12QS N5PH*12QK PH 5 µm 150 x 4.0 mm N5PH*15QS N5PH*15QK PH 5 µm 250 x 4.0 mm N5PH*25QS N5PH*25QK PH 7 µm 250 x 4.0 mm N7PH*25QS N7PH*25QK PH 5 µm 100 x 4.6 mm N5PH-10QS N5PH-10QK PH 5 µm 150 x 4.6 mm N5PH-15QS N5PH-15QK PH 5 µm 250 x 4.6 mm N5PH-25QS N5PH-25QK PH 7 µm 250 x 4.6 mm N7PH-25QS N7PH-25QK Diol OH 7 µm 250 x 4.0 mm N7OH*25QS N7OH*25QK100Å - 350 m2/g OH 7 µm 250 x 4.6 mm N7OH-25QS N7OH-25QK Amino NH2 5 µm 150 x 2.0 mm N5NH2#15QS N5NH2#15QK100Å - 350 m2/g NH2 5 µm 250 x 2.0 mm N5NH2#25QS N5NH2#25QK NH2 5 µm 150 x 3.0 mm N5NH2$15QS N5NH2$15QK NH2 5 µm 250 x 3.0 mm N5NH2$25QS N5NH2$25QK NH2 5 µm 125 x 4.0 mm N5NH2*12QS N5NH2*12QK NH2 5 µm 150 x 4.0 mm N5NH2*15QS N5NH2*15QK NH2 5 µm 250 x 4.0 mm N5NH2*25QS N5NH2*25QK NH2 5 µm 100 x 4.6 mm N5NH2-10QS N5NH2-10QK NH2 5 µm 150 x 4.6 mm N5NH2-15QS N5NH2-15QK NH2 5 µm 250 x 4.6 mm N5NH2-25QS N5NH2-25QK

Nucleosil®

QS QK



A.110

B.110


Nitro NO2 5 µm 150 x 2.0 mm N5NO2#15QS N5NO2#15QK100Å - 350 m2/g NO2 5 µm 250 x 2.0 mm N5NO2#25QS N5NO2#25QK NO2 5 µm 150 x 3.0 mm N5NO2$15QS N5NO2$15QK NO2 5 µm 250 x 3.0 mm N5NO2$25QS N5NO2$25QK NO2 5 µm 125 x 4.0 mm N5NO2*12QS N5NO2*12QK NO2 5 µm 150 x 4.0 mm N5NO2*15QS N5NO2*15QK NO2 5 µm 250 x 4.0 mm N5NO2*25QS N5NO2*25QK NO2 5 µm 100 x 4.6 mm N5NO2-10QS N5NO2-10QK NO2 5 µm 150 x 4.6 mm N5NO2-15QS N5NO2-15QK NO2 5 µm 250 x 4.6 mm N5NO2-25QS N5NO2-25QK Dimethylamino DMA 5 µm 250 x 2.0 mm N5DMA#25QS N5DMA#25QK100Å - 350 m2/g DMA 5 µm 250 x 3.0 mm N5DMA$25QS N5DMA$25QK DMA 5 µm 150 x 4.0 mm N5DMA*15QS N5DMA*15QK DMA 5 µm 250 x 4.0 mm N5DMA*25QS N5DMA*25QK DMA 5 µm 150 x 4.6 mm N5DMA-15QS N5DMA-15QK DMA 5 µm 250 x 4.6 mm N5DMA-25QS N5DMA-25QK Strong anionexchanger SB 5 µm 250 x 3.0 mm N5SB$25QS N5SB$25QK100Å - 350 m2/g SB 5 µm 250 x 4.0 mm N5SB*25QS N5SB*25QK1 meqv /g SB 5 µm 250 x 4.6 mm N5SB-25QS N5SB-25QK Strong cationexchanger SA 5 µm 250 x 3.0 mm N5SA$25QS N5SA$25QK100Å - 350 m2/g SA 5 µm 250 x 4.0 mm N5SA*25QS N5SA*25QK1 meqv /g SA 5 µm 250 x 4.6 mm N5SA-25QS N5SA-25QK Silica SI 5 µm 150 x 2.0 mm N5#15QS N5#15QK100Å - 350 m2/g SI 5 µm 250 x 2.0 mm N5#25QS N5#25QK SI 5 µm 150 x 3.0 mm N5$15QS N5$15QK SI 5 µm 250 x 3.0 mm N5$25QS N5$25QK SI 5 µm 125 x 4.0 mm N5*12QS N5*12QK SI 5 µm 150 x 4.0 mm N5*15QS N5*15QK SI 5 µm 250 x 4.0 mm N5*25QS N5*25QK SI 10 µm 250 x 4.0 mm N10*25QS N10*25QK SI 5 µm 100 x 4.6 mm N5-10QS N5-10QK SI 5 µm 150 x 4.6 mm N5-15QS N5-15QK SI 5 µm 250 x 4.6 mm N5-25QS N5-25QK SI 10 µm 250 x 4.6 mm N10-25QS N10-25QK

Nucleosil®

QS QK





A.111

B.111



Octadecyl OD3 5 µm 150 x 4.0 mm P5OD3*15QS P5OD3*15QK(polymérique) OD3 5 µm 250 x 4.0 mm P5OD3*25QS P5OD3*25QK85Å - 350 m2/g OD3 5 µm 150 x 4.6 mm P5OD3-15QS P5OD3-15QK%C : 10,5 OD3 5 µm 250 x 4.6 mm P5OD3-25QS P5OD3-25QKend-capped OD3 10 µm 150 x 4.0 mm P10OD3*15QS P10OD3*15QK1.45 µmol/m2 OD3 10 µm 250 x 4.0 mm P10OD3*25QS P10OD3*25QK OD3 10 µm 150 x 4.6 mm P10OD3-15QS P10OD3-15QK OD3 10 µm 250 x 4.6 mm P10OD3-25QS P10OD3-25QK Octadecyl OD2 5 µm 150 x 4.0 mm P5OD2*15QS P5OD2*15QK(polymérique) OD2 5 µm 250 x 4.0 mm P5OD2*25QS P5OD2*25QK85Å - 350 m2/g OD2 5 µm 150 x 4.6 mm P5OD2-15QS P5OD2-15QK%C : 16 OD2 5 µm 250 x 4.6 mm P5OD2-25QS P5OD2-25QKnon end-capped OD2 10 µm 150 x 4.0 mm P10OD2*15QS P10OD2*15QK2.43 µmol/m2 OD2 10 µm 250 x 4.0 mm P10OD2*25QS P10OD2*25QK OD2 10 µm 150 x 4.6 mm P10OD2-15QS P10OD2-15QK OD2 10 µm 250 x 4.6 mm P10OD2-25QS P10OD2-25QK Octadecyl OD1 10 µm 150 x 4.0 mm P10OD1*15QS P10OD1*15QK85Å - 350 m2/g OD1 10 µm 250 x 4.0 mm P10OD1*25QS P10OD1*25QK%C : 5 OD1 10 µm 150 x 4.6 mm P10OD1-15QS P10OD1-15QKend-capped OD1 10 µm 250 x 4.6 mm P10OD1-25QS P10OD1-25QK Octyl C8 5 µm 150 x 4.0 mm P5C8*15QS P5C8*15QK85Å - 350 m2/g C8 5 µm 250 x 4.0 mm P5C8*25QS P5C8*25QK%C : 8,5 C8 5 µm 150 x 4.6 mm P5C8-15QS P5C8-15QKend-capped C8 5 µm 250 x 4.6 mm P5C8-25QS P5C8-25QK2.33 µmol/m2 C8 10 µm 150 x 4.0 mm P10C8*15QS P10C8*15QK C8 10 µm 250 x 4.0 mm P10C8*25QS P10C8*25QK C8 10 µm 150 x 4.6 mm P10C8-15QS P10C8-15QK C8 10 µm 250 x 4.6 mm P10C8-25QS P10C8-25QK Amino - cyano PAC 5 µm 150 x 4.0 mm P5PAC*15QS P5PAC*15QK(2:1) PAC 5 µm 250 x 4.0 mm P5PAC*25QS P5PAC*25QK85Å - 350 m2/g PAC 5 µm 150 x 4.6 mm P5PAC-15QS P5PAC-15QK PAC 5 µm 250 x 4.6 mm P5PAC-25QS P5PAC-25QK PAC 10 µm 150 x 4.0 mm P10PAC*15QS P10PAC*15QK PAC 10 µm 250 x 4.0 mm P10PAC*25QS P10PAC*25QK PAC 10 µm 150 x 4.6 mm P10PAC-15QS P10PAC-15QK PAC 10 µm 250 x 4.6 mm P10PAC-25QS P10PAC-25QK

Partisil® 85 Å

Silica manufacturer : Whatman

Standard silica type A Irregular pH stability : 2 < pH < 7 Surface area : 350 m2/g

QS QK

A.112

B.112


Strong anion exchanger SAX 10 µm 150 x 4.0 mm P10SAX*15QS P10SAX*15QK SAX 10 µm 250 x 4.0 mm P10SAX*25QS P10SAX*25QK85Å - 350 m2/g SAX 10 µm 150 x 4.6 mm P10SAX-15QS P10SAX-15QK SAX 10 µm 250 x 4.6 mm P10SAX-25QS P10SAX-25QK Strong cation exchanger SCX 10 µm 150 x 4.0 mm P10SCX*15QS P10SCX*15QK SCX 10 µm 250 x 4.0 mm P10SCX*25QS P10SCX*25QK85Å - 350 m2/g SCX 10 µm 150 x 4.6 mm P10SCX-15QS P10SCX-15QK SCX 10 µm 250 x 4.6 mm P10SCX-25QS P10SCX-25QK Silica SI 5 µm 150 x 4.0 mm P5*15QS P5*15QK85Å - 350 m2/g SI 5 µm 250 x 4.0 mm P5*25QS P5*25QK SI 5 µm 150 x 4.6 mm P5-15QS P5-15QK SI 5 µm 250 x 4.6 mm P5-25QS P5-25QK SI 10 µm 150 x 4.0 mm P10*15QS P10*15QK SI 10 µm 250 x 4.0 mm P10*25QS P10*25QK SI 10 µm 150 x 4.6 mm P10-15QS P10-15QK SI 10 µm 250 x 4.6 mm P10-25QS P10-25QK

Partisil® 85 Å


Partisil-10 SAX - 4.6 mm x 25 cmMobile phase : 0.05MKH2PO4 pH 3.35Flow rate : 1.0 ml/minPressure : 700 psigDetection : UV@254nmInjection volume : 5.0 µl

Nucleotidesa : CMP (Cytidine-5’ monophosphate)b : AMP (Adenosine-5’ monophosphate)c : UMP (Cytidine-5’ monophosphate)d : GMP (Guanosine-5’ monophosphate)

Silica manufacturer : Whatman

Standard silica type A Irregular pH stability : 2 < pH < 7 Surface area : 350 m2/g


A.113

B.113



Octadecyl OD2 3 µm 150 x 2.0 mm SG3OD2#15QS SG3OD2#15QK80Å - 220 m2/g OD2 3 µm 150 x 3.0 mm SG3OD2$15QS SG3OD2$15QK%C : 11,8 OD2 3 µm 50 x 4.6 mm SG3OD2-5QS SG3OD2-5QKend-capped OD2 3 µm 100 x 4.6 mm SG3OD2-10QS SG3OD2-10QK OD2 3 µm 150 x 4.6 mm SG3OD2-15QS SG3OD2-15QK OD2 5 µm 150 x 2.0 mm SG5OD2#15QS SG5OD2#15QK OD2 5 µm 250 x 2.0 mm SG5OD2#25QS SG5OD2#25QK OD2 5 µm 150 x 3.0 mm SG5OD2$15QS SG5OD2$15QK OD2 5 µm 250 x 3.0 mm SG5OD2$25QS SG5OD2$25QK OD2 5 µm 125 x 4.0 mm SG5OD2*12QS SG5OD2*12QK OD2 5 µm 250 x 4.0 mm SG5OD2*25QS SG5OD2*25QK OD2 5 µm 50 x 4.6 mm SG5OD2-5QS SG5OD2-5QK OD2 5 µm 100 x 4.6 mm SG5OD2-10QS SG5OD2-10QK OD2 5 µm 150 x 4.6 mm SG5OD2-15QS SG5OD2-15QK OD2 5 µm 250 x 4.6 mm SG5OD2-25QS SG5OD2-25QK Octadecyl OD1 3 µm 150 x 2.0 mm SG3OD1#15QS SG3OD1#15QK80Å - 220 m2/g OD1 3 µm 150 x 3.0 mm SG3OD1$15QS SG3OD1$15QK%C : 6 OD1 3 µm 50 x 4.6 mm SG3OD1-5QS SG3OD1-5QKnon end-capped OD1 3 µm 100 x 4.6 mm SG3OD1-10QS SG3OD1-10QK OD1 3 µm 150 x 4.6 mm SG3OD1-15QS SG3OD1-15QK OD1 5 µm 150 x 2.0 mm SG5OD1#15QS SG5OD1#15QK OD1 5 µm 250 x 2.0 mm SG5OD1#25QS SG5OD1#25QK OD1 5 µm 150 x 3.0 mm SG5OD1$15QS SG5OD1$15QK OD1 5 µm 250 x 3.0 mm SG5OD1$25QS SG5OD1$25QK OD1 5 µm 125 x 4.0 mm SG5OD1*12QS SG5OD1*12QK OD1 5 µm 250 x 4.0 mm SG5OD1*25QS SG5OD1*25QK OD1 5 µm 50 x 4.6 mm SG5OD1-5QS SG5OD1-5QK OD1 5 µm 100 x 4.6 mm SG5OD1-10QS SG5OD1-10QK OD1 5 µm 150 x 4.6 mm SG5OD1-15QS SG5OD1-15QK OD1 5 µm 250 x 4.6 mm SG5OD1-25QS SG5OD1-25QK Octyl C8 5 µm 150 x 2.0 mm SG5C8#15QS SG5C8#15QK80Å - 220 m2/g C8 5 µm 250 x 2.0 mm SG5C8#25QS SG5C8#25QK%C : 6 C8 5 µm 150 x 3.0 mm SG5C8$15QS SG5C8$15QKend-capped C8 5 µm 250 x 3.0 mm SG5C8$25QS SG5C8$25QK C8 5 µm 125 x 4.0 mm SG5C8*12QS SG5C8*12QK C8 5 µm 250 x 4.0 mm SG5C8*25QS SG5C8*25QK C8 5 µm 50 x 4.6 mm SG5C8-5QS SG5C8-5QK C8 5 µm 100 x 4.6 mm SG5C8-10QS SG5C8-10QK C8 5 µm 150 x 4.6 mm SG5C8-15QS SG5C8-15QK C8 5 µm 250 x 4.6 mm SG5C8-25QS SG5C8-25QK

Siligel® 85 Å

QS QK

Silica manufacturer : Interchim

Standard silica type A Spherical pH stability : 2 < pH < 7 Surface area : 220 m2/g

A.114

B.114


Hexyl C6 5 µm 150 x 2.0 mm SG5C6#15QS SG5C6#15QK80Å - 220 m2/g C6 5 µm 250 x 2.0 mm SG5C6#25QS SG5C6#25QK%C : 4.8 C6 5 µm 150 x 3.0 mm SG5C6$15QS SG5C6$15QKend-capped C6 5 µm 250 x 3.0 mm SG5C6$25QS SG5C6$25QK C6 5 µm 125 x 4.0 mm SG5C6*12QS SG5C6*12QK C6 5 µm 250 x 4.0 mm SG5C6*25QS SG5C6*25QK C6 5 µm 50 x 4.6 mm SG5C6-5QS SG5C6-5QK C6 5 µm 100 x 4.6 mm SG5C6-10QS SG5C6-10QK C6 5 µm 150 x 4.6 mm SG5C6-15QS SG5C6-15QK C6 5 µm 250 x 4.6 mm SG5C6-25QS SG5C6-25QK Cyano CN 5 µm 150 x 2.0 mm SG5CN#15QS SG5CN#15QK80Å - 220 m2/g CN 5 µm 250 x 2.0 mm SG5CN#25QS SG5CN#25QK%C : 3 CN 5 µm 150 x 3.0 mm SG5CN$15QS SG5CN$15QKnon end-capped CN 5 µm 250 x 3.0 mm SG5CN$25QS SG5CN$25QK CN 5 µm 125 x 4.0 mm SG5CN*12QS SG5CN*12QK CN 5 µm 250 x 4.0 mm SG5CN*25QS SG5CN*25QK CN 5 µm 50 x 4.6 mm SG5CN-5QS SG5CN-5QK CN 5 µm 100 x 4.6 mm SG5CN-10QS SG5CN-10QK CN 5 µm 150 x 4.6 mm SG5CN-15QS SG5CN-15QK CN 5 µm 250 x 4.6 mm SG5CN-25QS SG5CN-25QK Phenyl PH 5 µm 150 x 2.0 mm SG5PH#15QS SG5PH#15QK80Å - 220 m2/g PH 5 µm 250 x 2.0 mm SG5PH#25QS SG5PH#25QK%C : 2.5 PH 5 µm 150 x 3.0 mm SG5PH$15QS SG5PH$15QKnon end-capped PH 5 µm 250 x 3.0 mm SG5PH$25QS SG5PH$25QK PH 5 µm 125 x 4.0 mm SG5PH*12QS SG5PH*12QK PH 5 µm 250 x 4.0 mm SG5PH*25QS SG5PH*25QK PH 5 µm 50 x 4.6 mm SG5PH-5QS SG5PH-5QK PH 5 µm 100 x 4.6 mm SG5PH-10QS SG5PH-10QK PH 5 µm 150 x 4.6 mm SG5PH-15QS SG5PH-15QK PH 5 µm 250 x 4.6 mm SG5PH-25QS SG5PH-25QK Amino NH2 5 µm 150 x 2.0 mm SG5NH2#15QS SG5NH2#15QK80Å - 220 m2/g NH2 5 µm 250 x 2.0 mm SG5NH2#25QS SG5NH2#25QK%C : 2 NH2 5 µm 150 x 3.0 mm SG5NH2$15QS SG5NH2$15QKnon end-capped NH2 5 µm 250 x 3.0 mm SG5NH2$25QS SG5NH2$25QK NH2 5 µm 125 x 4.0 mm SG5NH2*12QS SG5NH2*12QK NH2 5 µm 250 x 4.0 mm SG5NH2*25QS SG5NH2*25QK NH2 5 µm 50 x 4.6 mm SG5NH2-5QS SG5NH2-5QK NH2 5 µm 100 x 4.6 mm SG5NH2-10QS SG5NH2-10QK NH2 5 µm 150 x 4.6 mm SG5NH2-15QS SG5NH2-15QK NH2 5 µm 250 x 4.6 mm SG5NH2-25QS SG5NH2-25QK

Siligel® 85 Å

QS QK





A.115

B.115


Strong anion exchanger SAX 5 µm 250 x 4.6 mm SG5SAX-25QS SG5SAX-25QK80Å - 220 m2/g SAX 10 µm 250 x 4.6 mm SG10SAX-25QS SG10SAX-25QK Strong cation exchanger SCX 5 µm 250 x 4.6 mm SG5SCX-25QS SG5SCX-25QK80Å - 220 m2/g SCX 10 µm 250 x 4.6 mm SG10SCX-25QS SG10SCX-25QK Silica SI 5 µm 150 x 2.0 mm SG5#15QS SG5#15QK80Å - 220 m2/g SI 5 µm 250 x 2.0 mm SG5#25QS SG5#25QK SI 5 µm 150 x 3.0 mm SG5$15QS SG5$15QK SI 5 µm 250 x 3.0 mm SG5$25QS SG5$25QK SI 5 µm 125 x 4.0 mm SG5*12QS SG5*12QK SI 5 µm 250 x 4.0 mm SG5*25QS SG5*25QK SI 5 µm 50 x 4.6 mm SG5-5QS SG5-5QK SI 5 µm 100 x 4.6 mm SG5-10QS SG5-10QK SI 5 µm 150 x 4.6 mm SG5-15QS SG5-15QK SI 5 µm 250 x 4.6 mm SG5-25QS SG5-25QK


Siligel® 85 Å

QS QK



A.116

B.116

Siligel® 85 Å

QS QK





A.117

B.117



Octadecyl OD2 4 µm 125 x 4.0 mm SU4OD2*12QS SU4OD2*12QK100Å - 350 m2/g OD2 4 µm 250 x 4.0 mm SU4OD2*25QS SU4OD2*25QK%C : 21,6 OD2 4 µm 100 x 4.6 mm SU4OD2-10QS SU4OD2-10QKend-capped OD2 4 µm 150 x 4.6 mm SU4OD2-15QS SU4OD2-15QK4.09 µmol/m2 OD2 4 µm 250 x 4.6 mm SU4OD2-25QS SU4OD2-25QK Octadecyl OD1 4 µm 125 x 4.0 mm SU4OD1*12QS SU4OD1*12QK100Å - 350 m2/g OD1 4 µm 250 x 4.0 mm SU4OD1*25QS SU4OD1*25QK%C : 21 OD1 4 µm 100 x 4.6 mm SU4OD1-10QS SU4OD1-10QKnon end-capped OD1 4 µm 150 x 4.6 mm SU4OD1-15QS SU4OD1-15QK3.61 µmol/m2 OD1 4 µm 250 x 4.6 mm SU4OD1-25QS SU4OD1-25QK Octyl RPB 4 µm 125 x 4.0 mm SU4RPB*12QS SU4RPB*12QK100Å - 350 m2/g RPB 4 µm 250 x 4.0 mm SU4RPB*25QS SU4RPB*25QK%C : 11,5 RPB 4 µm 100 x 4.6 mm SU4RPB-10QS SU4RPB-10QKend-capped RPB 4 µm 150 x 4.6 mm SU4RPB-15QS SU4RPB-15QK3.55 µmol/m2 RPB 4 µm 250 x 4.6 mm SU4RPB-25QS SU4RPB-25QK Octyl C8 4 µm 125 x 4.0 mm SU4C8*12QS SU4C8*12QK100Å - 350 m2/g C8 4 µm 250 x 4.0 mm SU4C8*25QS SU4C8*25QK%C : 12,5 C8 4 µm 100 x 4.6 mm SU4C8-10QS SU4C8-10QKnon end-capped C8 4 µm 150 x 4.6 mm SU4C8-15QS SU4C8-15QK4.04 µmol/m2 C8 4 µm 250 x 4.6 mm SU4C8-25QS SU4C8-25QK Octyl C8E 4 µm 125 x 4.0 mm SU4C8E*12QS SU4C8E*12QK100Å - 350 m2/g C8E 4 µm 250 x 4.0 mm SU4C8E*25QS SU4C8E*25QK%C : 13 C8E 4 µm 100 x 4.6 mm SU4C8E-10QS SU4C8E-10QKend-capped C8E 4 µm 150 x 4.6 mm SU4C8E-15QS SU4C8E-15QK4.44 µmol/m2 C8E 4 µm 250 x 4.6 mm SU4C8E-25QS SU4C8E-25QK Silica SI 4 µm 125 x 4.0 mm SU4*12QS SU4*12QK100Å - 350 m2/g SI 4 µm 250 x 4.0 mm SU4*25QS SU4*25QK SI 4 µm 100 x 4.6 mm SU4-10QS SU4-10QK SI 4 µm 150 x 4.6 mm SU4-15QS SU4-15QK SI 4 µm 250 x 4.6 mm SU4-25QS SU4-25QK

Superspher® 100 Å

QS QK

Silica manufacturer : Merck


A.118

B.118


Yperspher® 120 Å


Spherical silica type A Spherical silica type A deactivated pH stability : 2 < pH < 7 Surface area : 180 m2/g

QS QK


Octadecyl C18 3 µm 150 x 2.0 mm YP3C18#15QS YP3C18#15QK120Å - 180 m2/g C18 3 µm 150 x 3.0 mm YP3C18$15QS YP3C18$15QK%C : 10 C18 3 µm 50 x 4.6 mm YP3C18-5QS YP3C18-5QKend-capped C18 3 µm 100 x 4.6 mm YP3C18-10QS YP3C18-10QK C18 3 µm 150 x 4.6 mm YP3C18-15QS YP3C18-15QK C18 5µm 150 x 2.0 mm YP5C18#15QS YP5C18#15QK C18 5µm 250 x 2.0 mm YP5C18#25QS YP5C18#25QK C18 5µm 150 x 3.0 mm YP5C18$15QS YP5C18$15QK C18 5µm 250 x 3.0 mm YP5C18$25QS YP5C18$25QK C18 5µm 125 x 4.0 mm YP5C18*12QS YP5C18*12QK C18 5µm 250 x 4.0 mm YP5C18*25QS YP5C18*25QK C18 5µm 50 x 4.6 mm YP5C18-5QS YP5C18-5QK C18 5µm 100 x 4.6 mm YP5C18-10QS YP5C18-10QK C18 5µm 150 x 4.6 mm YP5C18-15QS YP5C18-15QK C18 5µm 250 x 4.6 mm YP5C18-25QS YP5C18-25QK Octadecyl BDSC18 5µm 150 x 2.0 mm YP3BC18#15QS YP3BC18#15QK135Å - 180 m2/g BDSC18 5µm 150 x 3.0 mm YP3BC18$15QS YP3BC18$15QK deactivated BDSC18 5µm 50 x 4.6 mm YP3BC18-5QS YP3BC18-5QK%C : 11 BDSC18 5µm 100 x 4.6 mm YP3BC18-10QS YP3BC18-10QKend-capped BDSC18 5µm 150 x 4.6 mm YP3BC18-15QS YP3BC18-15QK BDSC18 5µm 150 x 2.0 mm YP5BC18#15QS YP5BC18#15QK BDSC18 5µm 250 x 2.0 mm YP5BC18#25QS YP5BC18#25QK BDSC18 5µm 150 x 3.0 mm YP5BC18$15QS YP5BC18$15QK BDSC18 5µm 250 x 3.0 mm YP5BC18$25QS YP5BC18$25QK BDSC18 5µm 125 x 4.0 mm YP5BC18*12QS YP5BC18*12QK BDSC18 5µm 250 x 4.0 mm YP5BC18*25QS YP5BC18*25QK BDSC18 5µm 50 x 4.6 mm YP5BC18-5QS YP5BC18-5QK BDSC18 5µm 100 x 4.6 mm YP5BC18-10QS YP5BC18-10QK BDSC18 5µm 150 x 4.6 mm YP5BC18-15QS YP5BC18-15QK BDSC18 5µm 250 x 4.6 mm YP5BC18-25QS YP5BC18-25QK Octyl C8 5µm 150 x 2.0 mm YP5C8#15QS YP5C8#15QK120Å - 180 m2/g C8 5µm 250 x 2.0 mm YP5C8#25QS YP5C8#25QK%C : 7 C8 5µm 150 x 3.0 mm YP5C8$15QS YP5C8$15QKend-capped C8 5µm 250 x 3.0 mm YP5C8$25QS YP5C8$25QK C8 5µm 125 x 4.0 mm YP5C8*12QS YP5C8*12QK C8 5µm 250 x 4.0 mm YP5C8*25QS YP5C8*25QK C8 5µm 50 x 4.6 mm YP5C8-5QS YP5C8-5QK C8 5µm 100 x 4.6 mm YP5C8-10QS YP5C8-10QK C8 5µm 150 x 4.6 mm YP5C8-15QS YP5C8-15QK C8 5µm 250 x 4.6 mm YP5C8-25QS YP5C8-25QK


A.119

B.119



Octyl BDSC8 5µm 150 x 2.0 mm YP5BC8#15QS YP5BC8#15QK135Å - 180 m2/g BDSC8 5µm 250 x 2.0 mm YP5BC8#25QS YP5BC8#25QK deactivated BDSC8 5µm 150 x 3.0 mm YP5BC8$15QS YP5BC8$15QK%C : 7 BDSC8 5µm 250 x 3.0 mm YP5BC8$25QS YP5BC8$25QKend-capped BDSC8 5µm 125 x 4.0 mm YP5BC8*12QS YP5BC8*12QK BDSC8 5µm 250 x 4.0 mm YP5BC8*25QS YP5BC8*25QK BDSC8 5µm 50 x 4.6 mm YP5BC8-5QS YP5BC8-5QK BDSC8 5µm 100 x 4.6 mm YP5BC8-10QS YP5BC8-10QK BDSC8 5µm 150 x 4.6 mm YP5BC8-15QS YP5BC8-15QK BDSC8 5µm 250 x 4.6 mm YP5BC8-25QS YP5BC8-25QK Cyano CN 5µm 150 x 2.0 mm YP5CN#15QS YP5CN#15QK120Å - 180 m2/g CN 5µm 250 x 2.0 mm YP5CN#25QS YP5CN#25QK%C : 4 CN 5µm 150 x 3.0 mm YP5CN$15QS YP5CN$15QKnon end-capped CN 5µm 250 x 3.0 mm YP5CN$25QS YP5CN$25QK CN 5µm 125 x 4.0 mm YP5CN*12QS YP5CN*12QK CN 5µm 250 x 4.0 mm YP5CN*25QS YP5CN*25QK CN 5µm 50 x 4.6 mm YP5CN-5QS YP5CN-5QK CN 5µm 100 x 4.6 mm YP5CN-10QS YP5CN-10QK CN 5µm 150 x 4.6 mm YP5CN-15QS YP5CN-15QK CN 5µm 250 x 4.6 mm YP5CN-25QS YP5CN-25QK Phenyl PH 5µm 150 x 2.0 mm YP5PH#15QS YP5PH#15QK120Å - 180 m2/g PH 5µm 250 x 2.0 mm YP5PH#25QS YP5PH#25QK%C : 5 PH 5µm 150 x 3.0 mm YP5PH$15QS YP5PH$15QKnon end-capped PH 5µm 250 x 3.0 mm YP5PH$25QS YP5PH$25QK PH 5µm 125 x 4.0 mm YP5PH*12QS YP5PH*12QK PH 5µm 250 x 4.0 mm YP5PH*25QS YP5PH*25QK PH 5µm 50 x 4.6 mm YP5PH-5QS YP5PH-5QK PH 5µm 100 x 4.6 mm YP5PH-10QS YP5PH-10QK PH 5µm 150 x 4.6 mm YP5PH-15QS YP5PH-15QK PH 5µm 250 x 4.6 mm YP5PH-25QS YP5PH-25QK

QS QK

Yperspher® 120 ÅSilica manufacturer : Interchim


A.120

B.120


Amino NH2 5µm 150 x 2.0 mm YP5NH2#15QS YP5NH2#15QK120Å - 180 m2/g NH2 5µm 250 x 2.0 mm YP5NH2#25QS YP5NH2#25QK%C : 2 NH2 5µm 150 x 3.0 mm YP5NH2$15QS YP5NH2$15QKnon end-capped NH2 5µm 250 x 3.0 mm YP5NH2$25QS YP5NH2$25QK NH2 5µm 125 x 4.0 mm YP5NH2*12QS YP5NH2*12QK NH2 5µm 250 x 4.0 mm YP5NH2*25QS YP5NH2*25QK NH2 5µm 50 x 4.6 mm YP5NH2-5QS YP5NH2-5QK NH2 5µm 100 x 4.6 mm YP5NH2-10QS YP5NH2-10QK NH2 5µm 150 x 4.6 mm YP5NH2-15QS YP5NH2-15QK NH2 5µm 250 x 4.6 mm YP5NH2-25QS YP5NH2-25QK Amino SI 5µm 150 x 2.0 mm YP5#15QS YP5#15QK120Å - 180 m2/g SI 5µm 250 x 2.0 mm YP5#25QS YP5#25QK SI 5µm 150 x 3.0 mm YP5$15QS YP5$15QK SI 5µm 250 x 3.0 mm YP5$25QS YP5$25QK SI 5µm 125 x 4.0 mm YP5*12QS YP5*12QK SI 5µm 250 x 4.0 mm YP5*25QS YP5*25QK SI 5µm 50 x 4.6 mm YP5-5QS YP5-5QK SI 5µm 100 x 4.6 mm YP5-10QS YP5-10QK SI 5µm 150 x 4.6 mm YP5-15QS YP5-15QK SI 5µm 250 x 4.6 mm YP5-25QS YP5-25QK


Yperspher® 120 Å

QS QK




A.121

B.121


Yperspher® 120 Å

QS QK



A.122

B.122



Octadecyl C18 5 µm 150 x 2.0 mm Z5C18#15QS Z5C18#15QK70Å - 330 m2/g C18 5 µm 250 x 2.0 mm Z5C18#25QS Z5C18#25QK%C : 20 C18 5 µm 150 x 3.0 mm Z5C18$15QS Z5C18$15QKend-capped C18 5 µm 250 x 3.0 mm Z5C18$25QS Z5C18$25QK3.47 µmol/m2 C18 5 µm 150 x 4.6 mm Z5C18-15QS Z5C18-15QK C18 5 µm 250 x 4.6 mm Z5C18-25QS Z5C18-25QK C18 7 µm 250 x 4.6 mm Z7C18-25QS Z7C18-25QK Octyl C8 5 µm 150 x 2.0 mm Z5C8#15QS Z5C8#15QK70Å - 330 m2/g C8 5 µm 250 x 2.0 mm Z5C8#25QS Z5C8#25QK%C : 10 C8 5 µm 150 x 3.0 mm Z5C8$15QS Z5C8$15QKend-capped C8 5 µm 250 x 3.0 mm Z5C8$25QS Z5C8$25QK2.99 µmol/m2 C8 5 µm 150 x 4.6 mm Z5C8-15QS Z5C8-15QK C8 5 µm 250 x 4.6 mm Z5C8-25QS Z5C8-25QK C8 7 µm 250 x 4.6 mm Z7C8-25QS Z7C8-25QK Methyl C1 5 µm 150 x 2.0 mm Z5C1#15QS Z5C1#15QK70Å - 330 m2/g C1 5 µm 250 x 2.0 mm Z5C1#25QS Z5C1#25QK%C : 5 C1 5 µm 150 x 3.0 mm Z5C1$15QS Z5C1$15QKnon end-capped C1 5 µm 250 x 3.0 mm Z5C1$25QS Z5C1$25QK4.79 µmol/m2 C1 5 µm 150 x 4.6 mm Z5C1-15QS Z5C1-15QK C1 5 µm 250 x 4.6 mm Z5C1-25QS Z5C1-25QK Phenyl PH 5 µm 150 x 2.0 mm Z5PH#15QS Z5PH#15QK70Å - 330 m2/g PH 5 µm 250 x 2.0 mm Z5PH#25QS Z5PH#25QK%C : 5 PH 5 µm 150 x 3.0 mm Z5PH$15QS Z5PH$15QKend-capped PH 5 µm 250 x 3.0 mm Z5PH$25QS Z5PH$25QK1.23 µmol/m2 PH 5 µm 150 x 4.6 mm Z5PH-15QS Z5PH-15QK PH 5 µm 250 x 4.6 mm Z5PH-25QS Z5PH-25QK Amino NH2 5 µm 150 x 2.0 mm Z5NH2#15QS Z5NH2#15QK70Å - 330 m2/g NH2 5 µm 250 x 2.0 mm Z5NH2#25QS Z5NH2#25QK%C : 4 NH2 5 µm 150 x 3.0 mm Z5NH2$15QS Z5NH2$15QKnon end-capped NH2 5 µm 250 x 3.0 mm Z5NH2$25QS Z5NH2$25QK2.21 µmol/m2 NH2 5 µm 150 x 4.6 mm Z5NH2-15QS Z5NH2-15QK NH2 5 µm 250 x 4.6 mm Z5NH2-25QS Z5NH2-25QK Silica SI 5 µm 150 x 2.0 mm Z5SI#15QS Z5SI#15QK70Å - 330 m2/g SI 5 µm 250 x 2.0 mm Z5SI#25QS Z5SI#25QK SI 5 µm 150 x 3.0 mm Z5SI$15QS Z5SI$15QK SI 5 µm 250 x 3.0 mm Z5SI$25QS Z5SI$25QK SI 5 µm 150 x 4.6 mm Z5SI-15QS Z5SI-15QK SI 5 µm 250 x 4.6 mm Z5SI-25QS Z5SI-25QK

Zorbax® 70 Å

Silica manufacturer : Agilent Technologies


QS QK


A.123

B.123


GP 5 µm polymeric columnsGP polymeric columns are packed with a porous styrene and divinyl benzene copolymer.This mechanically stable gel is hydrophobic in nature, in its native form (GP5RP) & stable under aggressive pH conditions - gels can achieve basic compound separation at high pH (> 8). Whilst this copolymer is less efficient than silica, good level of efficiences can, in part, be achieved through a tight pore and particle size distribution.

The modified polyamide bonding phase (GP5PA) is suitable for saccharides and polysaccharides.

Available in 5 µm for analytical applications.

Typical applications Dimension P/N

Reverse phase 150 x 4.6 mm GP5RP-15QS 250 x 4.6 mm GP5RP-25QS

Ion exchange, saccharides, polysaccharides 150 x 4.6 mm GP5PA-15QS 250 x 4.6 mm GP5PA-25QS

QS QK

Polymer Gel 100 Å

Manufacturer : Interchim

PSDVB ultra pure Spherical pH stability : 0 < pH < 14 Surface area : 400 m2/g

GP5RP-10QSSolvent : 55/37,2/7,8 ACN/H2O/MeOHpH 3,0 with 0.05 M Na2HPO4Flow rate : 2.0 ml/minTemp. : 25°CDetector : UV@254 nmInj. : 40 µl

GP5PA-25QSSolvent A : 85/15 ACN/H2O Solvent B : H2OGradient : 15 min Linear, 0 % B to 21 % BFlow rate : 1 ml/minTemp. : 25°CInj. : 100 µl of 1.0 % Conc.

Base Neutral acid Test Mix Corn Syrup

A.124

B.124

Analysis - HPLC - Interchim technologyInterchrom preparative columns

Preparative HPLCInterchim Preparative columns range from 4.6 to 50.8 mm i.d and are for the purification of samples ranging from 0.5 mg to 1 gram. Preparative HPLC places specific demands on the components that form a preparative column. Interchim's Modulo-cart preparative columns pay particular attention to both the component and collective manufacturing processes.

Column tubing & column packing The tube polishing value (Ra) has a fundamental importance in preparative chromatography. A primary reason for broadening peaks and low efficiency is the utilization of a poorer quality tubing. Molecules in the center of the mobile phase stream can move more rapidly than the molecules closer to the side due to friction against the tubing surface. The lower the Ra value, the smoother the surface is, and the less 'drag' the tubing will place upon a given separation. Modulo-cart preparative columns pay particular attention to this potential negative phenome-non. All columns have extremely smooth internal surfaces (typically 8 µ inch of Ra) to considerably reduce issues of drag and maintain column efficiency. Efficiency is also managed through Interchim's state-of-the art proprietary packing processes - Modulo-cart Prep withstand packing pressures up to 550 bars contributing strongly to a good bed stability and column life time.

Sample dispersionThe loading of sample onto a preparative column requires stringent management to establish quality separations. Column overloading results in a poor retention of pure fraction and therefore particular attention needs to be placed upon selecting the appropriate column dimension and the properties of the stationary phase. In addition, a careful control of the introduction of sample to the column is necessary to establish a homogeneous sample dispersion through the sorbent bead head. Sample typically enters a preparative column through a 1/16” fitting; poor sample loading will lead to overloa-ding certain areas of the stationary phase whilst other areas will be underloaded.E.g. For a 50 mm i.d column with a 500 µm i.d capillary fitting - sample introduced to the column (without any sample distributor) will only interact with 0.01% of the surface column head. As well as a dramatic loss in capacity there will also be a high potential for the column head to prematurely clog, rapidly reducing column life times. To prevent this problem Interchim's Modulo-cart Preparative columns are outfitted with a sample distributor. The sample distributor design maximizes the efficiency of sample volume dispersion and the sample mass introduced to the surface of the column head.


A.125

B.125


Stationary phaseUpti-prep® technology not only provides extreme mechanical stablity for all Uptisphere® stationary phases, stability is also assured for all particle sizes for a given stationary phase. Interchim’s proprietary Uptisphere® ( 320 m2/g) and Uptisphere® StrategyTM (425 m2/g) stationary phases guarantee perfect batch to batch reproducibility and feature high loading capacities in a range of different selectivities. The seamless retention of quality from analytical phases with sub 2, 3 and 5 µm silica particles to preparative 10 and 15 µm particles place Interchim’s Modulo-cart preparative range at the forefront of preparative HPLC.

Purification capacity for a 250 mm length column relative to internal diameter I.D. Purification capacity*

4.6 mm 0.5 to 10 mg 10.0 mm 2 to 50 mg 21.2 mm 20 to 200 mg 28.0 mm 50 to 400 mg 50.8 mm 100 mg to 1 g

*Capacity depends on the ratio : peak of interest Resolution (Rs) /impurities to purify

(Products and part number described later in the purification section)



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Description P/N Qty

Frit replacement tool CH347520 1

On-line filter0.5 µm T50270 102.0 µm R21281 10

C-clamps (Easy Modulo-Cart tighten /untightening) Column i.d. 1.0 & 2.0 mm T94280 13.0 - 4.0 - 4.6 mm R49870 1

Analysis - HPLC - Column protectionColumn & Guard cartridge

Column Protection - On-line filters and guard cartridges

HPLC column performance can suffer from sample impurities. A range of tools are available to protect the column and retain performance. The simple addition of a low porosity 0.5 or 2 µm on-line filter can protect from physical impurities whilst a guard cartridge is one of the best means to protect against chemical sample pollution. Guard cartridges are typically packed with the same phase as the HPLC column - they should be changed on a regular basis for optimal protection.

Description P/N Qty

Guard Cartridge holderDirect connection for Modulo-cart QS (10 mm) CH797540 1

1/16”- 1/16” standard CH980740 1

Guard cartridges 10 x 1,0 mm Q95961 310 x 1,0 mm Q95960 1010 x 2,0 mm CH980671 310 x 2,0 mm CH980670 1010 x 4,0 mm CH979521 310 x 4,0 mm CH979520 10

Caps/frits 2µm for 1.0 mm i.d CH980600 5for 2.0 mm i.d CH881940 5for 3.0 mm i.d CH881950 5for 3.9 mm i.d CH881960 5for 4.0 mm i.d CH881960 5for 4.6 mm i.d CH881970 5


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High performance guard column

Short length guard columns are packed with the stationary phase of your requirement, typically the same phase as the column (specify stationary phase when ordering). Even if the length of the guard column is relatively small, i.e. 20, 30 or 50 mm, it will perform as an analytical column, retention times are proportionally affected .

Analytical guard columnDirect analytical column fitting with no dead volume

Description Dimensions P/N Qty

Guard column 20 x 2.0 mm CHAK1110 1Replacement frits 2 µm. 2.0 mm i.d. CH098980 5Guard column 20 x 4.0 mm CHAK1120 1Replacement frits 2 µm. 4.0 mm i.d. CH099240 5

30 mm analytical guard cartridgeDescription P/N Qty

33 x 1.0 mm CAK113Q 133 x 2.1 mm CH86027Q 133 x 3.0 mm CH88845Q 133 x 4.0 mm CH89906Q 133 x 4.6 mm CH10912Q 1

10 mm preparative guard cartridgeDescription Dimensions P/N Qty

Guard holder 10 mm i.d. CH907780 1Guard cartridge 10 x 10 mm CH907770 1Guard holder 21.2 mm i.d. 923431 1Guard cartridge 10 x 21.2 mm 923421 1

30 and 50 mm preparative guard cartridgePreparative guard cartridges are typically packed with particle sizes > 10 µm (prices are relative to the type of stationary phase). Contact Interchim regarding frit replacement.

Description P/N Qty

33 x 7.8 mm CH73822Q 133 x 10.0 mm CH73825Q 133 x 21.2 mm CH73622Q 150 x 21.2 mm CH52379Q 150 x 28.0 mm CHAK107Q 1

Guard cartridge need to be used w/ QS connectors CH953820

Analysis - HPLC - Column protectionColumn & Guard cartridge

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Analysis - HPLCAqua Way™ Cation columns

This column is based on a thermo-responsive polymer synthesized and grafted onto 5 µm 120Ǻ silica beads. The surfaces of this grafted silica show charge properties at lower temperatures and, as the temperature in-creases, transform to uncharged surface properties. Selectivity is lead by controlling the column temperature. No gradient elution of high salt concentration is needed.

º Thermo-responsive bonding

Mechanism of the thermo-responsive beads :

Nucleotides

150 x 4.6 mm columnMobile phase : 66.7mM phosphate buffer (pH 7.0)Flow rate : 1.0 mL /minDetector : UV 254 mm

Samples :1. AMP2. ADP3. ATP

Description P/N

5 µm, 250 x 4.6 mm AWC0462505 µm, 150 x 4.6 mm AWC0461505 µm, 50 x 4.6 mm AWC046050


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Analysis - HPLCAqua Way™ Philic columns

This column is based on a thermo-responsive polymer synthesized and grafted onto 5 µm 120Ǻ aminopropyl silica. The surface of the grafted silica is hydrophobic at elevated temperatures and, as temperature decreases, transforms to hydrophilic surface properties. Selectivity and retention are lead by controlling the column tempe-rature. No acetonitrile required within the mobile phase.

º Thermo-responsive bonding

Small molecules on Aqua Way Philic

150mm x 4.6mm IDEluent : waterFlow rate : 1.0 mL /minDetector : UV 254 mm

Samples :1. Hydrocortisone2. Cortisone3. Prednisolone4. Prednisolone acetate5. Testosterone

Gradient effect through temperature control :

Description P/N

5 µm, 250 x 4.6 mm AWA0462505 µm, 150 x 4.6 mm AWA0461505 µm , 50 x 4.6 mm AWA046050

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Analysis - HPLCFluoroSep columns

FluoroSep is an ultrapure silica based media with hydrophobic fluorated silanes for reverse phase.It is compatible with all primary reverse phase eluents including ion pair reagents.

Two types of FluoroSep are available :

ºFluoroSep RP is adapted for the halogenated compound analysis. Columns facilitate the separation of aromtic compounds having isomers of position like pesticides and herbicides.

ºFluoroSep Octyl dedicated to the pharmaceutical compounds analysis, showing special selectivity in comparison with classical C8 sorbents.

Cephalosporin antibioticsFluoroSep-RPTM Phenyl 5 µ

60 Å, 25 cm x 4.6 mm Eluent : Acetonitrile : pH 4 acetate buffer 12:88Flow rate : 1.0 ml /minPressure : 103 barsDetector : UV @ 260 nm

Description FluoroSep phenyl FluoroSep octyl

Analytical columns w/5 µm particle size 250 x 4.6 mm 793410 545310150 x 4.6 mm 732030 732110100 x 4.6 mm 732130 CI097050 x 4.6 mm CI0890 CI0980

250 x 2.0 mm CI0900 CI0990150 x 2.0 mm CI0910 CI1000100 x 2.0 mm CI0920 CI101050 x 2.0 mm CI0930 CI1020

Analytical columns w/3 µm particle size 150 x 4.6 mm 73206050 x 4.6 mm CI0940

150 x 2.0 mm CI095050 x 2.0 mm CI0960


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Analysis - HPLCInertsil® columns - series

Inertsil® ODS-3 V columnsAll Inertsil® 5µ ODS-3 V columns are shipped with a silica batch certificate and an analysis certificate of the column. They guarantee that the column is manufactured according to "Good Laboratory Practice" and "Good Manufactoring Practice".

Description 1 column fromthe same batch

3 columns from the same batch

3 columns from three different batches

250 x 4.6 mm 5020-01802 5020-[4.6X250-1BATCH] 5020-[4.6X250-3BATCHES]150 x 4.6 mm 5020-01801 5020-[4.6X150-1BATCH] 5020-[4.6X150-3BATCHES]250 x 4.0 mm 5020-01812 5020-[4.0X250-1BATCH] 5020-[4.0X250-3BATCHES]150 x 4.0 mm 5020-01811 5020-[4.0X150-1BATCH] 5020-[4.0X150-3BATCHES]250 x 3.0 mm 5020-01822 5020-[3.0X250-1BATCH] 5020-[3.0X250-3BATCHES]150 x 3.0 mm 5020-01821 5020-[3.0X150-1BATCH] 5020-[3.0X150-3BATCHES]

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Analysis - HPLCInertsil® columns - series III

Inertsil® ODS-EP columns

Most reverse phases loose their retention capacity while the eluent contains a high amount of water.The inserted polar group of this bonding permits a special hydrophilic behaviour and then leads to reproducible analysis.

º100% water compatibleºAlternative selectivity to classical C18ºFiting to LC/MS analysis

[100% water eluent has no effect on the retention] Inertsil® ODS-EPAlkyl-C18

Internal diameter Length33 mm 50 mm 150 mm 250 mm

Analytical Columns0.2 mm 5020-01975-ODSEP-5U 5020-01976-ODSEP-5U0.3 mm 5020-01870-ODSEP-5U 5020-01871-ODSEP-5U0.5 mm 5020-01872-ODSEP-5U 5020-01873-ODSEP-5U1.0 mm 5020-18211 5020-18212 5020-18215 5020-182162.1 mm 5020-01975-ODSEP-5U 5020-02615 5020-026163.0 mm 5020-02625 5020-026264.0 mm 5020-02635 5020-026364.6 mm 5020-02642 5020-02645 5020-02646Preparative columns10 mm 5020-18252 5020-18256Guard column10 x 4.0 mm : 5020-08200-5U-ODS3


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Analysis - HPLCInertsil® columns - series III

Inertsil® ODS-P columnsThis silica sorbent modified with a polyfunctional bonding is dedicated to steric recognition separation. Molecules having different planar representation interact more or less with the bonding & are eluted successi-vely. This chemistry offers an alternative method for separation compared to a classical monofunctional C18.

ºSteric recognitonºEfficient PAH analysis

4.6 x 150mmEluent : Acetonitrile/Water (85:15)Flow rate : 1.0 mL /minTemperature : 40°CDetector : UV 254 nm

Internal diameter Length mm33 50 150 250

Analytical Column0.2 mm 5020-01975-ODSP-5U 5020-01976-ODSP-5U0.3 mm 5020-01870-ODSP-5U 5020-01871-ODSP-5U0.5 mm 5020-01872-ODSP-5U 5020-01873-ODSP-5U1.0 mm 5020-84711 5020-84712 5020-84715 5020-847162.1 mm 5020-04712 5020-04715 5020-047163.0 mm 5020-04725 5020-047264.0 mm 5020-04735 5020-047364.6 mm 5020-04742 5020-02001 5020-84715Preparative columns250 x 10 mm 5020-8475650 x 10 mm 5020-84752Guard column10 x 4.0mm : 5020-08200-5U-ODSP

Terphenyl isomer analysis showing the best ability to sepa-rate those compounds due to their spatial configuration. Results are compared to monofunctional ODS-2 & ODS-3.

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Analysis - HPLCInertsil® Sulfa C18 columns

Analysis of antibiotic drugs and drug residues in products of animal origin has become an important element of ensuring food safety.

Inertsil Sulfa C18 is suitable not only for the analyses of sulfa drugs but also for the scree-ning of synthetic anti-bacterial agents which are directed in the food safety investigate guideline.

ºAnti-bacterial agent analysis

Each column is tested with the following analytes and delivered with the relative chroma-togram.

Analyse of 15 sulfa reagents

System : GL-7400 HPLC systemColumn : Inertsil sulfa C18 (3µm, 150 x 4.6 mm I.D.)Eluent : A) CH3CN/CH3COOH=777.66 /3.15w/w B) CH3COOH/H O = 3.15:997 . w/w A/B = 0/100 - 33 min- 33/67 -3min- 90/10 -4min- 90/10Flow rate : 1.0 ml/minCol. Temp. : 40°CDetection : UV 270 nmInjection Vol. : 10 µL

1. Sulfanilamide2. Sulfacetamide3. Sulfadiazine4. Sulfathiazole5. Sulfapyridine6. Sulfacymidine8. Sulfamthoxypyridazine

9. Sulfamonomethoxine10. Sulfachlorpyridazine11. Sulfamethoxazole12. Sulfabenzamide13. Sulfadimethoxine14. Sulfaquinoxaline15. Sulfanitran

Description Sulfa C18

Analytical columns w/5 µm particle size250 x 4.6 mm 5020-07576150 x 4.6 mm 5020-07575250 x 3.0 mm 5020-07556150 x 3.0 mm 5020-07555150 x 3.0 mm 5020-07546150 x 3.0 mm 5020-07545

Analytical columns w/3 µm particle size150 x 4.6 mm 5020-07535100 x 4.6 mm 5020-07534150 x 3.0 mm 5020-07515100 x 2.1 mm 5020-07504


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Analysis - HPLCInertsil® ODS Sprint columns

Inertsil® ODS-Sprint is super base deactivated and optimally bonded to retain polar com-pounds without excessive retention of non-polar compounds. ODS-Sprint operates at low back pressure, even at high flow rates, showing outstanding longevity and reproducibility from column to column and batch to batch. To complement this new phase, we have created a zero-dead-volume column, hardware format, known as Sprint-HG hardware, further enhancing peak symmetry.

ºFast analysis of polar and non polar compounds

Description ODS Sprint

Analytical columns250 x 4.6 mm 5020-02746150 x 4.6 mm 5020-02745100 x 4.6 mm 5020-0274475 x 4.6 mm 5020-0274350 x 4.6 mm 5020-0274220 x 4.6 mm 5020-02741

250 x 2.1 mm 5020-02716150 x 2.1 mm 5020-02715100 x 2.1 mm 5020-0271475 x 2.1 mm 5020-0271350 x 2.1 mm 5020-0271220 x 2.1 mm 5020-02711

Guard column10 x 4.0 mm 5020-08200-5U-ODS-SP

System : GL7400 system HPLCEluent : CH3OH=80/20Flow rate : 1.0ml /minT° : 40°CDetector : 245nm

Samples : 1. Uracil2. Caffeine3. Phenol4. n-Butylbenzene5. o-Terphenyl6. n-Amylbenzene7. Triphenylene

Inertsil ODS-Sprint, 5u, 250 x 4.6mm

Inertsil ODS-3, 5u, 250 x 4.6mm

Hydrophobic compounds eluted in about half the time of Inertsil® ODS-3 while maintaining retention of polar compounds.

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Analysis - HPLCPrimesep™ 5 µm columns

Primesep™ columns feature double functionality of the bonding i.e : alkyl chain with anionic or cationic group, chelating group. This feature creates unique selectivities when using an appropriate mobile phase.

Columns operate in several acidic environments :

ºPrimesep A (ionized in all working pH) The most acidic sorbent. Strongly retains weak bases and amino compounds and presents a unique selectivity with neutral compounds.

ºPrimesep 100 (~ 50% of embedded acidic groups ionized at transition pH 1) The most universal column of the Primesep range. It can be used for the separation of basic, acidic, neutral or zwitterionic compounds. Acids are separated due to ion exclusion. Bases being controlled by cationic exchange.

ºPrimesep 200 (~ 50% of embedded acidic groups ionized at transition pH 2) Less acidic than Primesep 100, it is more suited to reverse phase mode. Selectivity is directly related to the use of less acidic mobile phases in order to favour basic compound elution.

Ion exchange and hydrophobic mechanism for separation of neurotransmitters on Primesep 200 column

Column : 150 x 3.2 mmEluent : H2O/meCN/TFA - 90/10/0.1Flow rate : 0.5 ml /minDetector : UV 210 nm

Bonding aspect under pH transition value of embedded acidic groups

Bonding aspect above pH transition value of embedded acidic groups

Description Primesep A Primesep 100 Primesep 200

Analytical columns250 x 4.6 mm A-46.250.0510 100-46.250.0510 200-46.250.0510150 x 4.6 mm A-46.150.0510 100-46.150.0510 200-46.150.051050 x 4.6 mm A-46.050.0510 100-46.050.0510 200-46.050.0510250 x 3.2 mm A-32.250.0510 100-32.250.0510 200-32.250.0510150 x 3.2 mm A-32.150.0510 100-32.150.0510 200-32.150.051050 x 3.2 mm A-32.050.0510 100-32.050.0510 200-32.050.0510250 x 2.1 mm A-21.250.0510 100-21.250.0510 200-21.250.0510150 x 2.1 mm A-21.150.0510 100-21.150.0510 200-21.150.051050 x 2.1 mm A-21.050.0510 100-21.050.0510 200-21.050.0510150 x 1.0 mm A-10.150.0510 100-10.150.0510 200-10.150.051050 x 1.0 mm A-10.050.0510 100-10.050.0510 200-10.050.0510

Preparative columns250 x 22 mm A-220.250.0510 100-220.250.0510 200-220.250.0510150 x 22 mm A-220.150.0510 100-220.150.0510 200-220.150.0510


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Analysis - HPLCPrimesep™ B 5 µm columns

All B-type columns provide at least two main interactions with analytes : the reverse-phase interaction and the anion-exchange interaction. Neutral analytes are retained by reverse phase interaction. The presence of the charged group in the alkyl chain provides additional selectivity uncommon for typical reverse-phase columns. Acidic analytes can be retained by both anion-exchange and reverse-phase mechanisms.

Columns are in three basic environments :

ºPrimesep B Primesep B is a strong basic column. The recommended pH range is from 1.5 to 4 created by the addition of trifluoroacetic, phosphoric, perchloric, or formic acid to the mobile phase.

ºPrimesep B2 Primesep B2 is a weak basic column. It also has carboxylic acidic functional group.When mobile phase pH is below 5, acidic groups are not ionized and B2 column surface becomes positively charged. This dual chemistry offers an extended pH range from 0.5 to 7, suitable for ammonium acetate and ammonium formate buffered mobile phases.

ºPrimesep B4 Primesep B4 is a reverse phase stationary phase with a short length modification that exhibits basic functionality. Separation of acidic compounds is via ion exchange and ion exclusion mechanisms facilitate the retention of hydrophobic base. This stationary phase is also relevant for acidic and basic surfactant analysis. pH stability 2 -to- 4.5

Both a cationic drug and chloride anion analyzed with single isocratic method

Primesep B : 150 x 3.2 mmFlow rate : 1.0 ml /minDetector : ELSD, UV 210 nmEluent : Water/MeCN/AmAc-40/60/0.2

Primesep B - Primesep B2Chemistry Schematic

Description Primesep B Primesep B2 Primesep B4Analytical columns250 x 4.6 mm B-46.250.0510 B2-46.250.0510 B4-46.250.0510150 x 4.6 mm B-46.150.0510 B2-46.150.0510 B4-46.150.051050 x 4.6 mm B-46.050.0510 B2-46.050.0510 B4-46.050.0510250 x 3.2 mm B-32.250.0510 B2-32.250.0510 B4-32.250.0510150 x 3.2 mm B-32.150.0510 B2-32.150.0510 B4-32.150.051050 x 3.2 mm B-32.050.0510 B2-32.050.0510 B4-32.050.0510250 x 2.1 mm B-21.250.0510 B2-21.250.0510 B4-21.250.0510150 x 2.1 mm B-21.150.0510 B2-21.150.0510 B4-21.150.051050 x 2.1 mm B-21.050.0510 B2-21.050.0510 B4-21.050.0510150 x 1.0 mm B-10.150.0510 B2-10.150.0510 B4-10.150.051050 x 1.0 mm B-10.050.0510 B2-10.050.0510 B4-10.050.0510

Semi preparative columns250 x 22 mm B-220.250.0510 B2-220.250.0510 B4-220.250.0510150 x 22 mm B-220.150.0510 B2-220.150.0510 B4-220.150.0510

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Analysis - HPLCPrimesep™ column comparison

Primesep™ columns differ in the degree they retain ionic compounds. Neutral compounds display similar retention on all Primesep columns. Primesep A, 100, 200 are cation exchange columns with different strengths of embedded functional groups. Primesep B and B2 are anion exchange columns. Primesep is a silica based material which is stable in all organic solvents and water at pH range from 1.0 to 7.5.

5µm, 150 x 4.6 mm columnFlow rate : 1,0 ml /minDetector : UV 210 nmEluent : Water/MeCN/TFA-60/40/0.1

Primesep A Primesep 100 Primesep 200

Primesep B Primesep B2 Common C18

Primesep columns comparison


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Analysis - HPLCPrimesep™ C 5 µm columns

Primesep™ C columns are the latest addition to the range which combine reverse-phase, cation exchange, and complex-formation properties in interaction with amines, sulfonium, phosphonium and metal ions.

This column has a unique unparallelled selectivity for separating complex mixtures.

The pH working range for these columns is from 1 to 7, but their complex formation and cation-exchange properties are substantially suppressed at a pH below 3 and therefore to facilitate the complex formation, the pH of the mobile phase should remain in the range of 3-7. The degree of complex formation can be adjusted by selecting the pH of the mobile phase.

ºComplex Interaction Schematic chemistry

Quaternary amines separated on Primesep C

Primesep C : 150 x 4.6 mm x 5 µmEluent : MeCN/H2O-15/85 TEA acetate 20 mM pH 5.0Flow rate : 1.0 ml /minDetector : ELSD, T° 35°C)Injection : 5 mcSample : 0.6 mg /ml each analyte

Description Primesep C

Analytical Columns250 x 4.6 mm C-46.250.0510150 x 4.6 mm C-46.150.051050 x 4.6 mm C-46.050.0510250 x 3.2 mm C-32.250.0510150 x 3.2 mm C-32.150.051050 x 3.2 mm C-32.050.0510250 x 2.1 mm C-21.250.0510150 x 2.1 mm C-21.150.051050 x 2.1 mm C-21.050.0510150 x 1.0 mm C-10.150.051050 x 1.0 mm C-10.050.0510

Semi preparative columns250 x 22 mm C-220.250.0510150 x 22 mm C-220.150.0510

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Analysis - HPLCPrimesep™ P 5 µm columns

Primesep™ P columns provide three unique interactions with analytes.

ºReverse phase interactionºp-p interactionºStrong cation exchange interaction

Depending on analyte properties, one, two, or all three interactions can be applied to the separation. This stationary phase facilitates the separation of structural isomers of aromatic compounds.Additional p-p interaction creates a difference in the bind state for structural isomers and often resolves critical pairs of compounds. The degree of p-p interaction can be adjusted by varying the amount of acetonitrile in the mobile phase.When methanol is used as an organic modifier, the highest degree of aromatic interaction can be achieved.

Description Primesep P

Analytical columns250 x 4.6 mm P-46.250.0510150 x 4.6 mm P-46.150.051050 x 4.6 mm P-46.050.0510250 x 3.2 mm P-32.250.0510150 x 3.2 mm P-32.150.051050 x 3.2 mm P-32.050.0510250 x 2.1 mm P-21.250.0510150 x 2.1 mm P-21.150.051050 x 2.1 mm P-21.050.0510150 x 1.0 mm P-10.150.051050 x 1.0 mm P-10.050.0510

Preparative columns250 x 22 mm P-220.250.0510150 x 22 mm P-220.150.0510

Chemistry Schematic


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Analysis - Carbohydrate analysisCARBOSep™ columns

CARBOSep™ columns employ a technique called ligand exchange chromatography for the separation of monosaccharides, disaccharides and oligosaccharides up to 15 glucose units long. Ligand exchange resins are highly sulfonated cation exchange resins that have group 1, 2 or transition series metals loaded on. This metal ion provides the positive charge that interacts with the negative charge on the sugar. Since the ionic charge is different for every sugar, separation of the sugars occurs.

CARBOSep™ columns are provided in a large variety of resin types and metals to provide selectivities that meet your separation needs.

Column featuresColumn Application Form Particle Size Mobile Phase Flow Rate Temperature

(typical) (recommended) (recommended)

CARBOSep CHO-411 Oligosaccharides up to DP 10. Corn syrup. molasses Sodium 20 µm Water 0.4 ml /min 75°CCARBOSep CHO-611 Oligosaccharides up to DP DP5 Sodium 10 µm Water 0.5 ml /min 90°CCARBOSep CHO-611-0H Mono and oligosaccharides w/PAD detection Sodium 10 µm Sodium hydroxide 0.5 ml /min 90°CCARBOSep CHO-620 Hight fructose corn syrup, mono-, ditrisaccharides and Calcium 10 µm Water 0.5 ml /min 90°C

sugars alcoholsCARBOSep CHO-682 Mono and disaccharides, sucrose, maltose, lactose Lead 7 µm Water 0.4 ml /min 80°CCARBOSep CHO-820 Simple sugars, sugars alcohols Calcium 8 µm Water 0.5 ml /min 90°C

CARBOSep COREGEL -42AG Oligosaccharides up to DP 11 Silver 20 µm Water 0.4 ml /min 75°CCARBOSep COREGEL 87 MM Mono, di, and trisaccharides. and sugars alcohols Cal/ sodium 8 µm Water 0.5 ml /min 85°CCARBOSep COREGEL 87C Mono and disaccharides Calcium 9 µm Water 0.6 ml /min 85°CCARBOSep COREGEL 87K Beet sugar, cane sugar, corn syrup, molasses Potassium 8 µm Water 0.6 ml /min 85°CCARBOSep COREGEL 87N Beet sugar, mono and oligosaccharides Sodium 8 µm Water 0.6 ml /min 85°CCARBOSep COREGEL 87P Pentose, hexose, monosaccharides, alcohols Lead 8 µm Water 0.8 ml /min 85°C

CARBOSep ION 300 Glucose and fructose in organic acid mixtures Hydrogen 8 µm Sulfuric acid 0.4 ml /min 70°CCARBOSep ION 310 Grape must analysis Hydrogen 8 µm Sulfuric acid 0.8 ml /min 50°CCARBOSep USP L19 USp L-19 specifications for separation of sorbitol Calcium 9 µm Water 0.2 ml /min 30°C

and mannitol

ICSep COREGEL 87H1 Fast analysis of organic acids, alcohols, sugar mixtures Hydrogen 9 µm Sulfuric acid 0.6 ml /min 85°CICSep COREGEL 87H3 Organic acids, alcohols.sugar mixtures Hydrogen 9 µm Sulfuric acid 0.6 ml /min 85°C

Mobile phase : 100% water Flow rate : 0.5 ml /min.Temperature : 90°C

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Analysis - Carbohydrate analysis

Selectivity chartCompound / retention (min)

CHO-620 CHO-611 CHO-682 Coregel 87H Coregel 87P Coregel 87N Coregel 87K Coregel 87C

Arabinose 10.64 11.08 23.95 12.08 16.32 12.64 14.72 13.92Arabitol 12.32 10.52 39.82 12.65 25.24 11.64 12.1 18.36Cellobiose 6.65 7.17 15.58 8.43 10.98 7.9 9.26 8.94Digitaxose 10.26 10.18 21.95 - 15.48 11.4 12.32 14.19Fructose 10.07 10.33 25.84 11.25 16.96 11.61 13.31 13.63Galactitol 13.05 10.23 52.43 11.8 31.6 11.15 11.61 20.46Galactose 9.58 10.22 22.32 11.12 15.16 11.44 13.36 13.82Glucose 8.72 9.53 19.14 10.57 13.38 10.72 12.55 11.17Lactitol 8.55 7.87 33.23 9.26 19.5 8.45 9.34 12.17Lactose 7.01 7.51 17.37 8.77 11.84 8.18 9.63 9.44Lactulose 7.57 7.85 20.7 9 13.24 8.48 10.08 10.17Maltitol 8.54 7.68 30.38 9 17.76 8.28 9.06 12.22Maltoheptose 4.66 4.6 11.74 6.96 8.52 5.84 6.61 7.28Maltohexose 4.78 4.87 12.24 7 8.8 5.94 6.74 7.38Maltopentose 5 5.08 13.08 7.1 9.34 6.11 7.02 7.53Maltotriose 5.91 6.22 15.17 7.72 10.54 6.98 8.16 8.28Maltose 6.89 7.37 16.61 8.57 11.54 8.08 9.48 9.17Maltotetrose 5.37 5.54 14.07 7.3 9.84 6.42 7.46 7.8Mannitol 11.84 9.9 40.03 11.66 24.98 10.81 11.42 17.81Mannose 9.79 10.27 25.5 11.13 16.76 11.57 13.74 12.76Melezitose 5.78 6.01 13.85 - 13.08 6.81 7.82 8.2Melibiose 6.99 7.46 17.63 8.56 12.02 8.19 9.72 9.36Myo-inositol 10.82 11.01 35.58 11.02 20.06 12.48 14.08 14.27Nitrate 4.5 4.2 10.3 6.85 8.4 5.7 6.4 7.3Raffinose 5.86 6.1 14.4 - 10.22 6.88 7.92 8.24Rhamnose 9.64 9.88 22.56 11.94 15.26 11.08 12.83 12.86Ribitol 10.94 10.13 30.72 12.44 20.44 11.26 11.84 15.55Sorbitol 13.64 10.38 56.56 11.77 33.4 11.32 11.86 21.34Sorbose 9.5 9.93 22.38 10.08 15.24 11.08 12.66 12.86Stachyose 5.28 5.39 13.41 - 9.58 6.33 7.28 7.77Sucrose 6.76 7.27 15.7 - 11.1 7.99 9.11 9.09Tagatose 11.53 10.29 - 11.15 20.8 11.36 12.82 16.46Trehalose 6.7 7.14 15.98 8.64 11.2 7.85 9.02 9.07Xylitol 13.93 11.01 51.15 12.82 31.1 12.16 12.64 21.3Xylose 9.56 10.34 20.64 11.32 14.42 11.77 13.69 12.32

Mobile Phase : 100% WaterFlow rate : 0.5 ml /minTemperature : 90°C


A.143

B.143


Coregel 87C (calcium form)

ºMonosaccharide and sugar alcohol analysis

Description P/N

Column 300 x 7.8 mm CHO-99-9860Guard column 50 x 7.8 mm CHO-99-356020mm guard cart kit (1 holder + 2 cartridges) CHO-99-2360Replacement guard cartridge 20 x 4 mm (2 units) CHO-99-1360

Coregel 87N (sodium form)

ºMonosaccharide and sugar alcohol rapid analysis

Description P/N

Column 300 x 7.8 mm CHO-99-1363Guard holder 20 mm CHO-99-1300Replacement guard cartridge 20 x 4 mm (2 units) CHO-99-1363

Coregel 87P (lead form)Description P/N

Column 300 x 7.8 mm CHO-99-9864Guard holder 20 mm CHO-99-1300Replacement guard cartridge 20 x 4 mm (2 units) CHO-99-1364

Coregel-87C

Column : 7.8 mm x 300Eluent : Distilled WaterFlow rate : 0.6 ml /minT° : 85°CPressure : 425 psiDetector : RI Range 18xInjection : 20µl

Samples :1. Raffinose2. Sucrose3. Lactulose4. Glucose5. Galactose6. Fructose7. Ribitol8. Mannitol9. Sorbitol

A.144

B.144


Coregel 87MM (calcium /sodium form)

Enhanced glucose, fructose and sugar alcohol analysis

Description P/N

Column 300 x 7.8 mm CHO-99-9865Guard holder 20 mm AXC-99-1300Replacement guard cartridge 20 x 4 mm (2 units) CHO-99-1365

Coregel 87K (potassium form)

Description P/N


Coregel 42AG (silver form)

Description P/N


Carbohydrate analysis on Coregel 87MM

Eluent : WaterFlow rate : 0.6 ml /minDetector : RI

Samples :1. Sucrose2. Glucose3. Fructose4. Arabinose

CARBOSep™ USP L19

Complies with USP L-19 specifications for the separation of sorbitol and mannitolSeparates a wide number of additional carbohydrates

Description P/NColumn 200 x 4.0 mm CHO-99-8453


A.145

B.145

High Fructose Corn Syrup on CHO-511

Analysis - Carbohydrate analysisOligosaccharides CARBOSep™ columns

CHO-611

ºSeparates by both ligand exchange and size exclusionºDesigned for the separation of oligosaccharides up to DP5ºReproducible separation of corn syrup

Description P/N

Column 300 x 6.5 mm CHO-99-9751

CHO-511

ºSeparates by both ligand exchange and size exclusionºDesigned for the separation of oligosaccharides up to DP6. Separate up to DP11 with two columns.ºImproved peak shapes over other oligosaccharide separation columnsºReproducible separation of corn syrup

Description P/N

Column 300 x 7.8 mm CHO-99-8500

CARBOSep™ CHO-411

ºSeparates by both ligand exchange and size exclusionºDesigned for the separation of oligosaccharides up to DP10ºReproducible separation of corn syrup

Description P/N

Column 300 x 7.8 mm CHO-99-9850

Sample Points Retention Time (min)

DP7+ 6.498DP7 7.877DP6 8.746DP5 9.658DP4 10.830DP3 12.384Maltose 14.700Glucose 18.749Fructose 20.246

A.146

B.146

Analysis - Organic acids analysisICSep™ columns

ICSep™ columns provide varying efficiencies and selectivities for the separation of weak acids by ion exclusion. Packings employed with ion exclusion are totally sulfonated polystyrene divinylbenzene copolymers. By sulfonating the polymer, the bead behaves as though it were a negatively charged sphere. Species that have a negative charge are repelled from from the media while uncharged species are allowed to enter the sphere and adsorb onto the beads. The mobile phases are usually low concentration acids such as sulfuric acid.

Selectivity chart for ion exclusion columnsCompound Coregel 87H at 85°C Coregel 64H at 65°C ION-300 at 65°C ORH-801 at 45°CAcetic acid 13.8 min 15.0 min 14.9 min 10.4 minAcetoacetic acid nd nd nd 10.2 minAconitic acid 8.6 min 9.8 min 10.7 min 7.2 minAcrylic acid 15.9 min 17.7 min 17.9 min 13.1 minAdipic acid 12.5 min 15.1 min 15.8 min 11.6 minButanol 32.9 min 35.1 min 25.2 min 18.4 minButyric acid 18.4 min 21.0 min 20.8 min 15.2 minCitraconic acid 10.1 min 11.0 min 11.5 min ndCitric acid 7.5 min 8.0 min 8.6 min 5.5 minEthanol 21.4 min 21.7 min 20.6 min 14.6 minFormic acid 12.9 min 13.8 min 13.9 min 9.6 minFumaric acid 11.5 min 13.4 min 14.7 min 10.0 min2-Furoic acid 22.1 min 26.9 min 29.0 min 22.0 minGlucoronic acid nd nd nd 5.3 minGlycolic acid 11.4 min 13.0 min 12.9 min 8.5 minGlycoxylic acid 9.2 min 9.7 min 10.3 min 6.5 minHydroxybutyric acid 12.8 min 14.0 min 14.1 min 9.5 minIsobutyric acid 17.3 min 19.6 min 19.5 min 14.0 minItaconic acid 11.1 min 12.8 min 13.4 min 9.1 minKeto-butyric acid nd nd 11.4 min 7.4 minKeto-glutaric 7.8 min 8.2 min nd 5.6 minKeto-valeric acid 11.7 min 12.6 min 13.1 min 8.6 minLactic acid 11.9 min 12.9 min 11.6 min 8.7 minMaleic acid 8.2 min 8.6 min 9.0 min 5.9 minMalic acid 8.8 min 9.6 min 10.3 min 6.6 minMalonic acid 9.3 min 10.0 min 10.7 min 6.9 minMethanol 18.7 min 19.0 min 18.7 min 12.9 minMethylglutaric acid 11.8 min 13.9 min 14.5 min 10.0 minMethylsuccinic acid 10.9 min 12.5 min 13.0 min 8.8 minOxalic acid 6.7 min 6.6 min nd 4.5 minPropanol 25.9 min 26.7 min 22.2 min 16.1 minPropionic acid 15.8 min 17.4 min 17.4 min 12.3 minPyruvic acid 9.2 min 9.5 min 9.9 min 6.3 minQuinic acid 9.4 min 10.3 min 11.4 min 6.9 minShikimic acid 10.5 min 11.8 min 12.9 min 8.2 minSuccinic acid 10.4 min 11.7 min 12.2 min 8.2 minTartaric acid 8.0 min 8.6 min 9.5 min 5.9 minFlow rate : 0.6 ml/minnd : no data


A.147

B.147

ICSep™ Coregel 87H (hydrogen form)

ICSep™ Ion 107H (hydrogen form)

º10% cross-linked polymer for efficient acid analysis

CARBOSep™ Coregel 64H (hydrogen form)

Description P/NColumn 300 x 7.8 mm ICE-99-986020 mm guard cart kit (1 holder and 2 cartridges) ICE-99-2360Replacement guard cartridge 20 x 4 mm (2 units) ICE-99-2370


Description P/NColumn 300 x 7.8 mm ICE-99-9861Column 100 x 7.8 mm ICE-99-586120 mm guard cart kit (1 holder and 2 cartridges) ICE-99-2361Replacement guard cartridge 20 x 4 mm (2 units) ICE-99-2371

Description P/NColumn 300 x 7.8 mm ICE-99-9866Guard holder 20 mm AXC-99-1300Replacement guard cartridge 20 x 4 mm (2 units) ICE-99-2366

ICSep™ Coregel USP L17 (hydrogen form)

ºComplies with USP L-17 specifications for the separation of citric, lactic, and acetic acidºSeparates a wide number of other organic acids

Description P/NColumn 240 x 4.1 mm ICE-99-8461

A.148

B.148


ICSep™ Ion 300

ºIdeal for Krebs cycle acid analysis


ICSep™ Ion 310

ºAcid and alcohol rapid analysis


ICSep™ WA1

ºAcid and sugar analysis in wine

Description P/NColumn 300 x 7.8 mm ICE-99-981020 mm guard cart kit (1 holder and 2 cartridges) ICE-99-3510Replacement guard cartridge 20 x 4 mm (2 units) ICE-99-1310

The following ICSep columns are specially dedicated for sugars and sugar-alcohols in fruit juices.Wine Analysis WA-1

Eluent : 0.0025 N Sulfuric AcidFlow rate : 6 ml /minT° : 45°CDetector : RIInjection : 20µl

Sample :1 Citric Acid (0,5 mg/ml)2 Tartaric Acid (2,0 mg/ml)3 Glucose (2,0 mg/ml)4 Malic Acid (1,0 mg/ml)5 Fructose (2,0 mg/ml)6 Succinic Acid (0,5 mg/ml)7 Lactic Acid (2,0 mg/ml)8 Glycerine (5,0 mg/ml)9 Acetic Acid (0,5 mg/ml)10 2,3-Butanediol (0,5 mg/ml)11 Isomer Impurity12 Ethanol (10,0 mg/ml)


A.149

B.149

Analysis - Ions analysisICSep™ columns

The resins used for anion and cation exchange chromatography in IC employ a functionalized, macroporous polystyrene divynil benzene polymer. Resins functionalized with quaternaly alkyl or alkynol ammonium groups are used with hydroxide or carbonate-based eluents for anion exchange. Resins functionalized with sulfonic acid or carboxylic acid groups are used with acidic eluents for cation exchange.IC columns have been designed to be compatible with the main part of Ion chromatography systems. They are an excellent alternative to columns which meet the EPA methods requirements.

ICSep™ AN300 (anion exchange)

ºDesigned for EPA 300 methodºPeek harware


Column AN 300 150 x 5.5 mm ANX-99-7613Guard cartridges /3u 10 x 3.0 mm ANX-99-0010Guard holder 10 mm ANX-99-0050

ICSep™ AN2 (anion exchange)

ºHigh capacity

ICSep™ CN2 (cation exchange)


Column AN 2 250 x 4.6 mm ANX-99-8515Guard cartridges /3u 10 x 3.0 mm ANX-99-0015Guard holder 10 mm ANX-99-0050


Column CN2 100 x 3.2 mm CTX-99-5250Column CN2 50 x 4.6 mm CTX-99-3550Guard cartridges / 2u 10 x 3.0 mm CTX-99-1350

Inorganic anions EPA 300.0 method

AN 300, PEEK : 150 x 5.5 mmEluent : 1.7 mM NaHCO3 1,8 mM Na2CO3Detector : Suppressed conductivitySample volume : 20 µlFlow rate : 2.0 ml /minT° : AmbientPressure : 500 psi

Samples :1. Fluorure (2 ppm)2. Chlorure (20 ppm)3. Nitrite (2 ppm)4. Bromure (2 ppm)5. Nitrate (10 ppm)6. Phosphate (2 ppm)7. Sulfate (60 ppm)

Transgenomic column

Application

ICSep AN300 F-, CI-, NO2-, Br-, NO3-, HPO4

2-, SO42-

By E.P.A. Method 300.0(a)ICSep AN1 F-, CI-, NO2

-, Br-, NO3-, HPO42-, SO4

2-,Low molecular weight, Organic acids in medium to high ionic strength matricesCr(III), Cr(VI) as CrO3-, CrO4

2-

ICSep ANSC Polyvalent Phosphates, Arsenates, Sulfite Selenate, Ar-senite, Selenite, F-, CI-, NO2

-, Br-, NO3-, HPO42-, SO4

2-,Low molecular weight, Organic acids

ICSep AN1SC F-, CI-, NO2-, Br-, NO3-, HPO4

2-, SO42-,

Low molecular weight, Organic acids in medium to high ionic strength matrices

ICSep AN2 Arsenate, Sulfite, Selenate, Arsenite, Selenite F-, CI-, NO2

-, Br-, NO3-, HPO42-, SO4

2-,Low molecular weight Organic acids

ICSep AN300B F-, CI-, NO2-, Br-, NO3-, HPO4

2-, SO42-, CIO2

-, CIO3-, BrO3

-

ICSep CN2 Li+, Na+, K+, Rb+, Cs+, Mg2+, Ca2+, NH4+, Cu2+, Ni2+, Zn2+, Co2+, Cd2+, Pb2+, Mn2+, Fe2+, Fe3+

A.150

B.150

Physiological fluid amino acid analysis with AMINOSep lithium AAA-99-6311

Column : lithiumFlow rate : 0.233 ml /minT° : 48-70-77 °CPressure : 655 psiDetector : UVInjection : 20µl

Analysis - Amino acids analysisAMINOSep™ columns

Ion exchange chromatography is a popular technique for amino acid analysis as retention time & quantification are both highly reproducible irrespective of the sample matrix. This unique matrix insensitivity is important when comparing results from different patients or batches of protein hydrolysate.

Amino acid columns are subjected to many different types of samples (blood, urine, growth media, animal feed, wine etc ..). Often they are introduced with minimum sample preparation. These columns use polystyrene /divynylbenzene co-polymers and are stable in temperature and in the pH range of 0 to 14.

AMINOSep™ lithium and sodium form (cation exchange)

ºAvailable for both physiological and protein hydrolysate amino acids


Sodium column for Gold Beckman system 200 x 4 mm AAA-99-6310Lithium column 100 x 4 mm AAA-99-6311Sodium column 120 x 4 mm AAA-99-6312Sodium column AminoSep AA-511 120 x 4.6 mm AAA-99-6554Sodium column AminoSep AA-511 150 x 4.6 mm AAA-99-7554Sodium column AminoSep AA-911 250 x 4.6 mm AAA-99-8553Guard cartridgesSodium guard cartridges /2 units 20 x 4 mm AAA-99-1312Lithium guard cartridges /2 units 20 x 4 mm AAA-99-1311Guard cartridges for AA511 /2 units 20 x 4 mm AAA-99-1354Guard cartridges for AA911 /2 units 20 x 4 mm AAA-99-1353Guard kit for AA-511(1 holder + 2 cartridges) AAA-99-2354Guard kit for AA-911(1 holder + 2 cartridges) AAA-99-2353Guard kit for sodium column (1 holder + 2 cartridges) AAA-99-2312Guard kit for lithium column (1 support + 2 cartridges) AAA-99-2311


A.151

B.151

Daicel chiral columns are the most widely used and referenced in the world. Due to their broad selectivity, good durability and high loading capacity. These stationary phases have become the leading chromatography tool for enantiomeric analysis and chiral separations.

Columns are available in two chemistries : coated and immobilized phases.

First generation columns are made with a spherical high quality silica support onto which the polymeric chiral selector is physically coated. Due to this characteristic, solvents should be carefully selected.

New generation columns consist of a silica backbone onto which the polymeric chiral selector has been immobilized. The immobilization of polysaccharide derivatives on the matrix facilitate universal solvent compatibility on this highly selective chiral phase.

Chiralpak® IAamylose tris (3,5 dimethylphenylcarbamate)

ºImmobilized chiral selectorºEnhanced robustness and durability ºLarger choice of solvents

Description CHIRALPAK® IA, 5 µm

Analytical columns 150 x 4.6 mm 80324250 x 4.6 mm 80325

Microbore150 x 2.1 mm 80394

Guard cartridge (x3)10 x 4.6 mm 80311

Analysis - ChiralDaicel columns

A.152

B.152

Chiralpak® IBcellulose tris (3,5 dimethylphenylcarbamate)


Chiralpak® IC


Description Chiralpak® IB, 5 µmAnalytical columns150 x 4.6 mm 81324250 x 4.6 mm 81325

Microbore150 x 2.1 mm 81394

Guard cartridge (x3)10 x 4.0 mm 81311

Description Chiralpak® IC, 5 µmAnalytical columns150 x 4.6 mm 83324250 x 4.6 mm 83325



A.153

B.153

Description Particle Size 150 x 2.1 mm 50 x 4.6 mm 150 x 4 mm 150 x 4.6 mm 250 x 4.6 mm Guard cart. 10 x 4 mm (3 u)

Chiralcel® CA-1 10025Chiralcel® OA 10 µm 11025Chiralcel® OB 10 µm 12025Chiralcel® OB-H 5 µm 12324 12325 12311Chiralcel® OC 10 µm 13025Chiralcel® OD 10 µm 14025Chiralcel® OD-H 5 µm 14394 14324 14325 14311Chiralcel® OD-R 10 µm 14625 14011Chiralcel® OD-RH 5 µm 14794 14724 14711Chiralcel® OF 10 µm 15025Chiralcel® OG 10 µm 16025Chiralcel® OJ 10 µm 17025Chiralcel® OJ-H 5 µm 17394 17324 17325 17311Chiralcel® OJ-RH 5 µm 17794 17724 17711Chiralcel® OK 10 µm 18022 18025Chiralpak® AD 10 µm 19094 19025Chiralpak® AD-H 5 µm 19394 19324 19325 19311Chiralpak®AD-RH 5 µm 19794 19724 19711Chiralpak® AS 10 µm 20094 20025Chiralpak® AS-H 5 µm 20394 20324 20325 20311Chiralpak® AS-RH 5 µm 20794 20724 20711Chiralpak® IA 5 µm 80394 80324 80325 80311Chiralpak® IB 5 µm 81394 81324 81325 81311Chiralpak® MA(+) 3 µm 21822Chiralpak® OP(+) 10 µm 22025Chiralpak® OT(+) 10 µm 23022 23025Chiralpak® QD-AX 5 µm 31394 31324 31311 (/u)Chiralpak® QN-AX 5 µm 32394 32324 32311 (/u)Chiralpak® WH 10 µm 25625Crownpak® CR(-) 5 µm 28714 27711 (/u)Crownpak® CR(+) 5 µm 27714

Other selectivities


A.154

B.154

Bonded PhasesAmines, hydrocarbons, pesticides,PCBs, phenols, sulfur compounds,

100% dimethylpolysiloxane Nonpolar a : -60 to 325/350b : -60 to 300/320c : -60 to 260280

UBP1, DB-1, BP-1, HP-1 CP-Sil5, Rtx-1, OV-1,007-1

Alkaoids, drugs, FAMEsHalogenated compounds,Semi-volatiles, pesticides

5% phenyl - 95 % dimethyl polysiloxane

Nonpolar a : -60 to 325/350b : -60 to 300/320c : -60 to 260/280

UB5P, DB-5, DB-5ms, DB-5ht,XTI-5, CP-Sil8CB, 007-2, OV-5,

Rtx-5, Rtx-5, BPX-5, BP-5, SPB-5

Aroclors, alcohols, pesticides, VOAs

6% cyanopropyl-phenyl94% dimethyl polysiloxane

Mid-polar a : -20 to 280/300b : -20 to 260/280

UB1301, DB-1301, DB-624,Rtx-624, Mtx-1301, CP-624,

RTX-1301Aroclors, Amines, pesticidespharmaceuticals

35% dimethyl polysiloxane Mid-polar a : 40 to 300/320 DB-35, RTX-35, SPB-35, AT-35

Volatile compounds Mid-polar a : 260/260 UB624, DB-624, OV624, ZB-624HP-VOC, CP-Select624CB

Pesticides, herbicides, TMSSugars, aroclors

14 % cyanopropyl-phenyl86 % dimethyl polysiloxane

Mid-polar a : -20 to 280/300b : -20 to 260/280

UB1701, DB-1701, Rtx-1701, CP-Sil 19 CB, OV1701

Drugs, glycols, pesticides,steroids

50% phenyls, 50% methyl-polysiloxane

Mid-polar a : 40 to 280/300b : 40 to 260/280

UB17, UB17P, DB-17, DB-17ht,CP-Sil24CB, BPX200, 007-17,

OV-17Aldehydes, pesticides,Herbicides, organo-chlorineOrgano-phosphorous

Polar a : -45 to 240/260b : -45 to 220/240

UB210, DB-210, Rtx-200

FAMEs, alditol acetatesNeutral sterols

50 % cyanoproyl-phenyl50 % methyl-polysiloxane

PolarPolar

a : 40 to 220/240b : 40 to 200/220

UB225, DB-225, SP-2330, OV-225, Rtx-225, BP-225, 007-225

Solvents, glycols, alcohols Polyethylene Polar a : 20 to 250/260b : 20 to 230/240

UBWAX, DB-WAX, Carbowax 20MRtx-WAX, ZB-WAX, HP-20M

Acids, alcohols, aldehydesAcrylates, ketones, nitriles

Polyethylene glycol-acid Polar a : 60 to 240/250b : 60 to 230/240

UBFFAP, DB-FFAP, Stabilwax DA007-FFAP, BP21,

Applications Composition Polarity Temp. Range Phases with Similar Polarity Isothermal/ Programmed

a : d.i. < 530 µm - b : d.i. : 530 µm - c : d.i. > 2 µm

Stationary phase selection guide

Analysis - GC Capillary ColumnsUptiBondTM GC capillary columns

UptiBondTM is Interchim's proprietary GC capillary column technology that has been developed around rigorous, innovative manufacturing processes from the highest quality raw material. Every step of column manufacturing is strictly controlled, establishing a new standard in GC chromatography. UptiBondTM exhibits enhanced column to column reproducibility and the utilization of Interchim's in-house chemistry resources establish major product benefits such as ultra low levels of column bleeding.


A.155

B.155


UB1P - [100% Dimethyl Polysiloxane]

Low bleed MS grade column Suitable upgrade from : DB-1, HP-1, Ultra-1, SPB-1, CP-Sil5CB, ZB-1, OV1 Excellent general purpose column Hydrocarbon, pesticides, phenols, amines, flavors and fragrances I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.25 325/350 UB1P1525250.25 30 0.25 325/350 UB1P3025250.25 60 0.25 325/350 UB1P602525 0.32 15 0.25 325/350 UB1P1532250.32 30 0.25 325/350 UB1P3032250.32 60 0.25 325/350 UB1P603225

UB5P - [5% phenyl - 95% Dimethyl Polysiloxane]

Low bleed MS grade column Suitable Upgrade from : DB-5, DB-5ms, HP-5, HP-5ms, SPB-5, VF-5ms, ZB-5, OV5, Rtx-5, Rtx-5ms,Rtx-5SilMS Excellent general purpose column Alkaloids, drugs, FAMEs, halogened compounds, aromatic compounds, pesticides

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.10 325/350 UB5P1525010.25 15 0.25 325/350 UB5P1525250.25 15 0.50 325/350 UB5P1525500.25 30 0.10 325/350 UB5P3025010.25 30 0.25 325/350 UB5P3025250.25 30 0.50 325/350 UB5P3025500.25 30 1.0 325/350 UB5P3025100.25 60 0.10 325/350 UB5P6025010.25 60 0.25 325/350 UB5P602525 0.32 15 0.10 325/350 UB5P1532010.32 15 0.25 325/350 UB5P1532250.32 15 0.50 325/350 UB5P1532050.32 30 0.10 325/350 UB5P3032010.32 30 0.25 325/350 UB5P3032250.32 30 0.50 325/350 UB5P3032050.32 60 0.10 325/350 UB5P6032010.32 60 0.25 325/350 UB5P603225

A.156

B.156

UB17P - [50% phenyl - 50% Dimethyl Polysiloxane]

Ideal for pesticide analysis Polar compounds, FAMEs, free phenols, chlorinated pesticides, basic drugs, phatalate esters Suitable upgrade from : DB-17, DB-17ms, Rtx-50, ZB-50, CP-Sil19 CB, BPX-50, 007-17

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.25 320/350 UB17P1525250.25 30 0.25 320/350 UB17P3025250.25 60 0.25 320/350 UB17P602525 0.32 15 0.25 320/350 UB17P1532250.32 30 0.25 320/350 UB17P3032250.32 60 0.25 320/350 UB17P603225



A.157

B.157


UB1301 - [6% Cyanopropylphenyl - 94% Dimethylpolysiloxane]

Ideal for pesticide analysis Polar compounds, FAMEs, free phenols, chlorinated pesticides, basic drugs, phatalate esters Suitable Upgrade from : DB-1301, Rtx-1301, CPSil1301 etc..

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.25 280/300 UB13011525250.25 15 0.5 280/300 UB13011525500.25 15 1 260/280 UB13011525100.25 30 0.25 280/300 UB13013025250.25 30 0.5 280/300 UB13013025050.25 30 1 260/280 UB13013025100.25 60 0.25 280/300 UB13016025250.25 60 0.5 280/300 UB13016025500.25 60 1 260/280 UB1301602510 0.32 15 0.25 280/300 UB13011532250.32 15 0.5 280/300 UB13011532050.32 15 1 260/280 UB13011532100.32 30 0.25 280/300 UB13013032250.32 30 0.5 280/300 UB13013032050.32 30 1 260/280 UB13013032100.32 60 0.25 280/300 UB13016032250.32 60 0.5 280/300 UB13016032050.32 60 1 260/280 UB1301603210 0.53 15 1 260/280 UB13011553100.53 30 1 260/280 UB1301305310

UB624

UB624 is tested with volatile halogenated compounds, making it an excellent choice for Method 624, 501, 503.1, 524.2, 601-603 and 8260. Suitable Upgrade from : DB-624, Rtx-624, CP-Select 624CB, SPB-624, BP-624, AT-624, ZB-624

Volatile organic compounds (VOCs), residual solvents, volatile aromatic and unsaturated hydrocarbons.

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 30 1.4 260/260 UB6243025140.25 60 1.4 260/260 UB624602514 0.32 30 1.8 260/260 UB6243032180.32 30 3 260/260 UB6243032300.32 60 1.8 260/260 UB624603218 0.53 30 3 260/260 UB6243053300.53 75 3 260/260 UB624755330

A.158

B.158

UB1701 - [14% Cyanopropylphenyl - 86% Methylpolysiloxane]

Suitable upgrade from : DB-1701, Rtx-1701, SPB-1701, CP-Sil19CB, OV-1701, 007-1701, AT-1701 Pesticide organochlorides, Sugars, TMS, Triazines, Herbicides, Drugs

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.25 280/300 UB17011525250.25 15 0.50 280/300 UB17011525050.25 15 1.0 260/280 UB17011525100.25 30 0.25 280/300 UB17013025250.25 30 0.50 280/300 UB17013025050.25 30 1.0 260/280 UB17013025100.25 60 0.25 280/300 UB17016025250.25 60 0.50 280/300 UB17016025050.25 60 1.0 260/280 UB1701602510 0.32 15 0.25 280/300 UB17011532250.32 15 0.50 280/300 UB17011532050.32 15 1.0 260/280 UB17011532100.32 30 0.25 280/300 UB17013032250.32 30 0.25 280/300 UB17013032250.32 30 0.50 280/300 UB17013032050.32 30 1.0 260/280 UB17013032100.32 60 0.25 280/300 UB17016032250.32 60 0.50 280/300 UB17016032050.32 60 1.0 260/280 UB1701603210 0.53 15 1.0 260/280 UB17011553100.53 30 1.0 260/280 UB1701305310



A.159

B.159


UB17P - [50% Diphenyl – 50% Dimethylpolysiloxane]

Ideal for pesticide analysis Polar compounds, FAMEs, free phenols, chlorinated pesticides, basic drugs, phatalate esters Suitable upgrade from : DB-17, DB-17ms, Rtx-50, ZB-50, CP-Sil19 CB, BPX-50, 007-17

I.D mm Length M Thickness µm Temp. Range (°C) P/N 0.25 15 0.25 320/340 UB171525250.25 30 0.25 320/340 UB173025250.25 60 0.25 320/340 UB17602525 0.32 30 0.25 320/340 UB173032250.32 60 0.25 320/340 UB17603225 0.53 15 1.0 300/320 UB171553100.53 30 1.0 300/320 UB17305310 UB210I.D mm Length M Thickness µm Temp. Range (°C) P/N0.25 30 0.25 240/260 UB210302525

UB225I.D mm Length M Thickness µm Temp. Range (°C) P/N0.25 30 0.25 240/260 UB225302525

A.160

B.160


UBWAX - [Polyethylene Glycol]

I.D mm Length M Thickness µm Temp. Range (°C) P/N0.25 15 0.25 250/260 UBWAX1525250.25 30 0.25 250/260 UBWAX3025250.25 30 0.50 250/260 UBWAX3025050.25 60 0.25 250/260 UBWAX6025250.25 60 0.50 250/260 UBWAX602505 0.32 15 0.25 250/260 UBWAX1532250.32 30 0.25 250/260 UBWAX3032250.32 30 0.50 250/260 UBWAX3032050.32 60 0.25 250/260 UBWAX6032250.32 60 0.50 250/260 UBWAX603205 0.53 15 1.0 230/240 UBWAX1553100.53 15 2.0 230/240 UBWAX1553200.53 30 1.0 230/240 UBWAX3053100.53 30 2.0 230/240 UBWAX305320

UBAmines

I.D mm Length M Thickness µm Temp. Range (°C) P/N0.32 15 265/300 UBAMIN15320.32 30 265/300 UBAMIN30320.32 60 265/275 UBAMIN6032

UBFFAP - [Polyethylene Glycol]

I.D mm Length M Thickness µm Temp. Range (°C) P/N0.25 15 0.25 230/240 UBFFAP1525250.25 30 0.25 230/240 UBFFAP3025250.25 30 0.50 230/240 UBFFAP3025050.25 60 0.25 230/240 UBFFAP6025250.25 60 0.50 230/240 UBFFAP6025050.32 30 0.25 230/240 UBFFAP3032250.32 60 0.25 230/240 UBFFAP6032250.53 15 1.0 220/230 UBFFAP1553100.53 30 1.0 220/230 UBFFAP305310


Interchim can supply packed columns made with any combination of phases

Premium grade stainless tubing (only manufactured for GC only)Including brass Swagelock fittingsDelivery 2 or 3 weeks

For order enquiries please indicate :1. Type of tubing required i.e SS or Glass2. Length and OD of the column3. Loading % of liquid phase4. Nature of liquid phase5. Solid support and particle size (mesh)6. Name and model of GC chromatograph

For GC glass columns please specify your column configuration according to the drawing highlighted above.

Order enquiry example

6' x 1/8" Stainless steel 10% OV1 Chromosorb WMP 80/100 for HP 6890

Length OD Tubing type Percentage Liquid phase Solid support Mesh size GC model

Standard SS Uptipacked

Available from stock 1.8 m x 1/8"

Description P/N

10% Apiezon L on Chromosorb W-HP 80/100 79398010% Carbowax 1500 on Chrom. W-HP 80/100 81652010% Carbowax 20 M on Chromosorb W-HP 80/100 24569110% Carbowax 20 M on Chromosorb W-AW 80/100 18463010% Carbowax 20 M-TPA on Chrom. W-AW 80/100 742060Chromosorb 101, 80/100 834260Chromosorb 102, 80/100 24963010% DEGS on Chromosorb W-AW 80/100 7872305% FFAP on Chromosorb W-AW 80/100 8358503% OV-1 on Chromosorb W-HP 80/100 765440

Description P/N

10% OV-1 on Chromosorb W-HP 80/100 8679803% OV-17 on Chromosorb W-HP 80/100 39309010% OV-17 on Chromosorb W-HP 80/100 2225403% OV-101 on Chromosorb W-HP 80/100 74380010% OV-101 on Chromosorb W-HP 80/100 8020703%OV-225 on Chromosorb W-HP 80/100 84844010% OV-225 on Chromosorb W-HP 80/100 8828703% SE-30 GC Grade on Chromosorb W-HP 80/100 388810Porapak Q, 80/100 520800Porapak N, 80/100 978770

A.161

B.161

Analysis - GC Capillary ColumnsUptipackedTM GC columns

A.162

B.162

Analysis - GC Capillary ColumnsUptiBondTM GC capillary columns - applications

UptiBondTM UB1P

Compound Rt (min)

1 2,3-Butanediol 4.159 et 4.293 2 n-Decane 9.0193 1-Octanol 10.2544 2,6-Dimethylphenol 10.8295 1-Nonanal 10.9206 n-Undecane 11.357

Compound Rt (min)

7 2-Ethylhexanoic acid 11.5998 2,6-Dimethylaniline 12.0789 Methyl decanoate 15.98410 Dicyclohexylamine 18.07411 Methyl undecanoate 18.07412 Methyl dodecanoate 20.058

UB1P303225 / N° Series : 1P-53542Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm/sec (FID)

UptiBondTM UB5P

UB1301303225 / N° Series : 1301-62915Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm /sec (FID)

UptiBondTM UB1301

Compound Rt (min)

1 2,3-Butanediol 6.096 et 6.249 2 n-Decane 8.9223 n-Undecane 11.2544 1-Octanol 11.627

Compound Rt (min)

5 1-Nonanal 12.1486 2,6-Dimethylphenol 13.1857 2,6-Dimethylaniline 14.0288 2-Ethylhexanoic acid 14.6099 Methyl decanoate 16.80210 Dicyclohexylamine 18.52111 Methyl undecanoate 18.86112 Methyl dodecanoate 20.816

Compound Rt (min)

1 2,3-Butanediol 4.742 et 4.901 2 n-Decane 9.3323 1-Octanol 10.9964 n-Undecane 11.724

Compound Rt (min)

5 1-Nonanal 11.8126 2,6-Dimethylphenol 11.9147 2-Ethylhexanoic acid 12.2038 2,6-Dimethylaniline 13.3149 Methyl decanoate 16.80510 Dicyclohexylamine 18.92611 Methyl undecanoate 18.92612 Methyl dodecanoate 20.938

UB5P303225 / N° Series : 5P-61700Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm/sec (FID)

Interchim columns are tested according Grob test process. Each compound of this test mixture measures, efficency, film thickness, acid & basic characteristics and column activity.


A.163

B.163

UptiBondTM UB624

Compound Rt (min)

1 2,3-Butanediol 10.245 et 10.4692 n-Decane 14.0903 n-Undecane 16.7624 1-Octanol 17.103

Compound Rt (min)

5 1-Nonanal 17.8476 2,6-Dimethylphenol 18.9737 2-Ethylhexanoic acid 19.3438 2,6-Dimethylaniline 20.1399 Methyl decanoate 22.77110 Methyl undecanoate 24.95611 Dicyclohexylamine 25.09012 Methyl dodecanoate 27.023

UB624303218 / N° Series : 624-62911Temp. : 40°C to 230°C at 6°C /minCarrier Gas : Helium, 20 cm /sec (FID)

UptiBondTM UB1701

Compound Rt (min)

1 2,3-Butanediol 7.842 et 8.0792 n-Decane 8.7403 n-Undecane 11.0814 1-Octanol 12.866

Compound Rt (min)

5 1-Nonanal 13.2386 2,6-Dimethylphenol 15.2007 2-Ethylhexanoic acid 15.6508 2,6-Dimethylaniline 15.9149 Methyl decanoate 17.60610 Dicyclohexylamine 19.18911 Methyl undecanoate 19.65512 Methyl dodecanoate 21.601

UB1701303225 / N° Series : 170162914Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm /sec (FID)

UptiBondTM UBWAX

Compound Rt (min)

1 n-Decane 4.5532 n-Undecane 5.9733 1-Nonanal 11.7714 2,3-Butanediol 14.765 et 15.477

Compound Rt (min)

5 1-Octanol 14.9996 Methyl decanoate 15.7027 Dicyclohexylamine 16.7108 Methyl undecanoate 17.6129 Methyl dodecanoate 19.44510 2,6-Dimethylaniline 20.40311 2,6-Dimethyphenol 21.44712 2-Ethylhexanoic acid 22.090

UBWAX303225 / N° Series : WAX-62918Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm /sec (FID)

UptiBondTM UBFFAP

Compound Rt (min)

1 n-Decane 4.5622 n-Undecane 5.9743 1-Nonanal 11.8464 2,3-Butanediol 14.905 et 15.617

Compound Rt (min)

5 1-Octanol 15.0626 Methyl decanoate 15.7457 Dicyclohexylamine 17.6508 Methyl undecanoate 17.6509 Methyl dodecanoate 19.47710 2,6-Dimethylaniline 20.59211 2,6-Dimethyphenol 21.60812 2-Ethylhexanoic acid 22.179

UBFFAP303225 / N° Series : FFAP-62916Temp. : 40°C to 190°C at 6°C /minCarrier Gas : Helium, 20 cm /sec (FID)


A.164

B.164

Fatty acid methyl ester

1 - 3.129 C8:0 2 - 3.623 C9:0 3 - 4.182 C10:0 4 - 4.789 C11:0 5 - 5.487 C12:0

UB5-Premium 0.25 µm, 30 m x 0.25 mmCarrier Gas : He, 25 cm /sec, constant flowOven : 130°C (hold 1 min)200°C at 20°C /min (hold 2 min)Injection : 1 µl, split 1:100, 270°CDetection : FID, 280°C

Alcohols

1 - 2.671 Methanol2 - 2.884 Ethanol3 - 3.025 Acetone4 - 3.106 Isopropanol5 - 4.040 Butanone6 - 7.071 3-Methylbutan-1-ol7 - 7.163 2-Methylbutan-1-ol8 - 7.816 Pentan-1-ol

UB1-Premium 0.25µm, 30 m x 0.25 mmCarrier Gas : He, 25 cm /sec, constant flowOven : 30°C (hold 4 min)100°C at 10°C /minInjection : 1 µl, split 1:100, 200°CDetection : FID, 200°C

Solvents

1 - 2.555 Acetone2 - 3.018 Butanone3 - 3.628 Benzene4 - 5.074 Pentanol5 - 6.847 Ethylbenzene6 - 7.039 p-Xylene7 - 7.430 Styrene8 - 7.480 Cyclohexanone

UB5-Premium 0.25µm, 30 m x 0.25 mmCarrier Gas : He, 25 cm /sec, constant flowOven : 30°C (hold 3 min)100°C at 10°C /min (hold 2 min)Injection : 1 µl,split 1:100, 250°CDetection : FID, 300°C



A.165

B.165

UB5-Premium 0.25 µm, 30 m x 0.25 mmCarrier Gas : He, 25 cm /sec, constant flowOven : 35°C (hold 7.5 min)200°C at 15°C /min (hold 5 min)Injection : 1 µl, split 1:100, 250°CDetection : FID, 250°C

Hydrocarbons 1 - 2.511 unknown 2 - 3.680 Hexane 3 - 4.812 Benzene 4 - 5.944 Heptane 5 - 9.721 Octane 6 - 11.183 Ethylbenzene 7 - 11.988 Nonane 8 - 13.562 Decane 9 - 14.802 Undecane 10 - 15.870 Dodecane 11 - 16.839 Tridecane

HAPs

1- Naphthalene2- 2-Methylnaphthalene3- 1-Methylnaphthalene4- Acenaphthylene5- Acenaphthene6- Biphenyl

7- Fluorene8- Phenanthrene9- Anthracene10- Fluoranthene11- Pyrene12- Chrysene13- Benzo[a]

anthracene14- Benzo[b]anthracene15- Benzo[k]anthracene17- Benzo[e]pyrene18- Benzo[e]

fluoranthene19- Dibenz[a,h]anthracene20- Benzo[g,h,i]perylene21- Indeno[1,2,3-cd]pyrene

UB17P 0.25 mm I.D. x 30 m df = 0.25 µmOven : 80°C (3 min hold) ; 5°C/min 340°C (5 min hold)Detection : MSSample Conc. : each 10 mg/L

Mixture

UBWAX 0.25 mm I.D. x 60 m df = 0.25 µmOven : 40°C (5 min hold) 4°C /min 230°CInjection : SplitDetection : FID

16- Chloroform17- i-Butanol18- Tetrahydrofuran19- Methyl cellosolve20- 1,1,1-Trichlo-roethane21- 1,2-Dichloroethane22- i-Propyl acetate23- Benzene24- n-Butanol25- Carbon tetra-chloride26- Trichloroethylene27- 1,4-Dioxane28- Ethyl cellosolve29- n-Propyl acetate30- i-Amyl alcohol31- Methyl i-butyl

ketone32- n-Amyl alcohol33- Toluene34- i-Butyl acetate35- N,N-Dimethylfor-mamide36- Methyl n-butyl ketone37- n-Butyl acetate38. Tetrachloroethy-lene39- Chlorobenzene40- Ethylbenzene41- m-Xylene42- p-Xylene43- i-Amyl Acetate44- Cyclohexanol45- Styrene

46- Cyclohexanone47- o-Xylene48- Cellosolve acetate49- Butyl cellosolve50- n-Amyl acetate51- 1,1,2,2-tetrachlo-roethane52- Methylcyclo-hexanol53- Methylcyclohexa-none54- PHenol55- o-Dichloroben-zene56- o-Cresol57- m-Cresol58- p-Cresol

1- Methanol2- Ethanol3- Acetone4- i-Propanol5- Ethyl ether6- Methyl acetate7- Dichloromethane8- Carbon disulfide9- n-Propanol10- trans-1,2-Dichlo-roethylene11- Methyl ethyl ketone12- Hexane13- 2-Butanol14- cis_1,1-Dichloroethy-lene15- Ethyl acetate


A.166

B.166

UB Amines 0.32 mm I.D. x 60 mOven : 50°C (hold 3 min) 10°C /min 220°C (5 min. Hold)Detection : FID 250°CInjecteur : 260°CSplit ratio : 1:50Samples : solvents & amines

1- Methanol2- Dimethylamine3- Ethanol4- Acetonitrile5- Acetone6- Iso-Propanol7- Acetic acid8- Diethylamine9- Ethylacetate10- Triethylamine11- Pyridine12- Dimethylformamide13- Toluene

Mixture

Xylene compounds

1- p-Xylene2- m-Xylene3- o-Xylene4- Styrene

UBFFAP 0.25 mm I.D. x 60 m df = 0.5 µmOven : 150°CInjection : 250°CDetection : FID 250°C

Fragrances

UB5-Premium 0.25 µm, 30 m x 0.25 mmCarrier Gas : He, 0.1 cm /sec, constant flowOven : 40°C ; 85°C at 100°C /min ; 180°C at 3°C /minInjection : 1 µl, split 1:100, 250°CDetection : FID, 250°C



A.167

B.167

Amino acids analysis

Amino acids standard

Mushroom tissue with nitrogen

Mushroom tissue with nitrogen

UB5-Premium 0.25µm, 30 m x 0.25 mmCarrier Gas : Helium 1.2 ml /minOven : 110°C for 3 min110°C - 260°C at 6°C /min260°C for 4 minInjection : 250°C1/10 µl injectionDetection : MSD

Sample preparation :- Lyophilization of mushrooms- Ethanol extraction- Dry evaporation- Dissolution in HCl- Dry evaporation- Derivatization with MTBSTFA/ DMF (5/1) 3 min 83°C


Analysis - Cromlab Analysis_TR.pdf · Steric selectivity, the ability to separate molecules which...

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