An Illuminized Normal template for Word 2007+  · Web view☐Vortex plate at 1600 rpm for 1...

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Infinium HTS Lab Tracking Form, Auto Protocol 1 of 14 1 Quantitate DNA (Optional) Run Information Date Time Operator Robot Information Robot ID WG#-DNA Plate Plate 1 ID Plate 2 ID Plate 3 ID Standard DNA Plate Plate ID QNT Plate Plate 1 ID Plate 2 ID Plate 3 ID Comments PN 15047410 Rev A

Transcript of An Illuminized Normal template for Word 2007+  · Web view☐Vortex plate at 1600 rpm for 1...

Page 1: An Illuminized Normal template for Word 2007+  · Web view☐Vortex plate at 1600 rpm for 1 minute. ☐Centrifuge plate to 280 × g at 22°C for 1 minute. ☐Centrifuge plate to

Infinium HTS Lab Tracking Form, Auto Protocol 1 of 11

1 Quantitate DNA (Optional)Run Information

Date

Time

Operator

Robot InformationRobot ID

WG#-DNA PlatePlate 1 IDPlate 2 IDPlate 3 ID

Standard DNA PlatePlate ID

QNT PlatePlate 1 IDPlate 2 IDPlate 3 ID

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 2 of 11

2 Amplify DNA (Pre-Amp)Run Information

Date

Time

Operator

Robot InformationRobot IDPlate Position on Robot Bed

Batch InformationBatch NumberNumber of Samples

PlatesWG#-DNA Plate IDMSA3 Plate ID

Reagent Lot Numbers and Bar CodesMA1MA2MSM0.1N NaOH

☐Vortex plate at 1600 rpm for 1 minute.

☐Centrifuge plate to 280 × g at 22°C for 1 minute.

☐Centrifuge plate to 280 × g at 22°C for 1 minute.

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 3 of 11

WG#-DNA Sample IDsColumns 1 - 4 of the Microtiter Plate

1 2 3 4

ABCDEFGH

Columns 5 - 8 of the Microtiter Plate

5 6 7 8

ABCDEFGH

Columns 9 - 12 of the Microtiter Plate

9 10 11 12

ABCDEFGH

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 4 of 11

3 Incubate DNA (Post-Amp)Incubate in Hyb Oven for 20-24 Hours at 37°C.

Start Time

Stop Time

4 Fragment DNA (Post-Amp)Run Information

Date

Time

Operator

Robot InformationRobot ID

Plate Position on Robot Bed

Reagent Lot Numbers and Bar CodesFMS

☐Centrifuge tubes to 280 × g.

☐Centrifuge plate to 50 × g for 1 minute.

☐Vortex plate at 1600 rpm for 1 minute.

☐Centrifuge plate to 50 × g for 1 minute.

Heat Block at 37°C for One HourStart Time

Stop Time

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 5 of 11

5 Precipitate DNA (Post-Amp)Run Information

Date

Time

Operator

Robot InformationRobot ID

Plate Position on Robot Bed

Reagent Lot Numbers and Bar CodesPM1

100% 2 Propanol

100% 2 Propanol Date Opened

☐Centrifuge plate to 280 × g for 1 minute.

☐Vortex plate at 1600 rpm for 1 minute.

Incubate at 37°C for 5 minutes.Start Time

Stop Time

☐Centrifuge plate to 50 × g for 1 minute.

Incubate at 4°C for 30 minutes.Start Time

Stop Time

☐Centrifuge plate to 3000 × g at 4°C for 20 minute.

Air Dry at 22°C for 1 HourStart Time

Stop Time

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 6 of 11

6 Resuspend DNA (Post-Amp)Run Information

Date

Time

Operator

Robot InformationRobot ID

Plate Position on Robot Bed

Reagent Lot Numbers and Bar CodesRA1

☐Vortex plate at 1800 rpm for 1 minute.

☐Centrifuge plate to 280 × g.

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 7 of 11

7 Hybridize DNA (Post-Amp)Run Information

Date

Time

Operator

Robot InformationRobot ID

Reagent Lot Numbers and Bar CodesPB2

Heat Block at 95°C for 20 minutesStart Time

Stop Time

☐MSA3 plate cool down for 30 minutes at room temperature

☐Centrifuge MSA3 plate to 280 × g

Robot InformationPlate Position on Robot Bed

BeadChip 1 Serial Number and Position

BeadChip 2 Serial Number and Position

BeadChip 3 Serial Number and Position

BeadChip 4 Serial Number and Position

☐Centrifuge MSA3 plate to 280 × g.

Hyb Oven Incubation at 48°C for 16 to 24 hoursStart Time

Stop Time

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 8 of 11

Track BeadChips 1-4 for the 24x1 HTS BeadChipAdjustable Spacer Multi-Channel Pipette

Sample Section Naming Diagram

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 9 of 11

8 Wash BeadChip (Post-Amp)Run Information

Date

Time

Operator

Reagent Lot Numbers and Bar CodesPB1

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 10 of 11

9 Extend and Stain (XStain) BeadChip (Post-Amp)Run Information

Date

Time

Operator

Robot InformationRobot ID

Reagent Lot Numbers and Bar CodesRA1

LX1

LX2

EML

XC3

SML

SML Temperature

ATM

PB1

XC4

Alconox Powder Detergent

EtOH

EtOH Date Opened

95% formamide/1mM EDTA

Dry in Desiccator > 675 mm Hg (0.9 bar) for 50-55 minutesStart Time

Stop Time

Comments

PN 15047410 Rev A

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Infinium HTS Lab Tracking Form, Auto Protocol 11 of 11

10 Image BeadChip (Post-Amp)Run Information

Operator

BeadChip 1Barcode Number

Scanner ID

Image Date

BeadChip 2Barcode Number

Scanner ID

Image Date

BeadChip 3Barcode Number

Scanner ID

Image Date

BeadChip 4Barcode Number

Scanner ID

Image Date

Comments

PN 15047410 Rev A


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An Illuminized Normal template for Word 2007+ Web view☐MSA3 plate cool down for 30 minutes at room temperature ☐Centrifuge MSA3 plate to 280 × g. Robot Information. Plate Position

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